AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0609470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609470 MtrunA17_Chr4g0042111 94.484 562 31 0 1 562 1 562 0.0 1119
Msa0609470 MtrunA17_Chr7g0236991 55.904 542 236 2 22 562 14 553 0.0 647
Msa0609470 MtrunA17_Chr7g0237001 55.904 542 236 2 22 562 14 553 0.0 646
Msa0609470 MtrunA17_Chr7g0237041 55.904 542 236 2 22 562 14 553 0.0 646
Msa0609470 MtrunA17_Chr7g0237081 55.904 542 236 2 22 562 14 553 0.0 646
Msa0609470 MtrunA17_Chr7g0237051 55.904 542 236 2 22 562 14 553 0.0 646
Msa0609470 MtrunA17_Chr5g0425371 55.191 549 242 3 16 562 7 553 0.0 643
Msa0609470 MtrunA17_Chr3g0105071 55.556 558 244 3 7 562 2 557 0.0 642
Msa0609470 MtrunA17_Chr4g0024691 56.364 550 237 2 14 562 14 561 0.0 641
Msa0609470 MtrunA17_Chr6g0451711 54.098 549 250 2 15 562 10 557 0.0 637
Msa0609470 MtrunA17_Chr5g0431571 55.576 547 239 3 18 562 5 549 0.0 634
Msa0609470 MtrunA17_Chr6g0465911 54.219 557 249 3 7 562 2 553 0.0 634
Msa0609470 MtrunA17_Chr3g0082691 54.234 555 250 3 10 562 1 553 0.0 633
Msa0609470 MtrunA17_Chr6g0466051 54.039 557 250 3 7 562 2 553 0.0 631
Msa0609470 MtrunA17_Chr5g0431651 53.734 549 251 3 16 562 5 552 0.0 624
Msa0609470 MtrunA17_Chr6g0465441 52.364 550 258 3 15 562 6 553 0.0 619
Msa0609470 MtrunA17_Chr7g0236961 57.347 490 209 0 73 562 9 498 0.0 607
Msa0609470 MtrunA17_Chr7g0237011 57.347 490 209 0 73 562 9 498 0.0 607
Msa0609470 MtrunA17_Chr6g0465851 54.615 520 233 2 44 562 1 518 0.0 600
Msa0609470 MtrunA17_Chr2g0319041 45.552 562 292 10 8 562 3 557 1.75e-175 507
Msa0609470 MtrunA17_Chr7g0236971 57.107 401 172 0 162 562 1 401 2.79e-173 496
Msa0609470 MtrunA17_Chr8g0336181 46.739 552 283 8 18 562 9 556 1.20e-172 500
Msa0609470 MtrunA17_Chr2g0319051 45.946 518 272 7 49 562 1 514 2.14e-165 480
Msa0609470 MtrunA17_Chr6g0465981 56.614 378 158 3 7 383 2 374 2.82e-153 444
Msa0609470 MtrunA17_Chr6g0465871 56.614 378 158 3 7 383 2 374 2.82e-153 444
Msa0609470 MtrunA17_Chr6g0466081 56.614 378 158 3 7 383 2 374 2.82e-153 444
Msa0609470 MtrunA17_Chr6g0466111 57.478 341 142 2 44 383 1 339 2.17e-140 409
Msa0609470 MtrunA17_Chr7g0236951 59.813 321 127 1 63 383 1 319 1.02e-139 407
Msa0609470 MtrunA17_Chr7g0237031 59.813 321 127 1 63 383 1 319 2.21e-139 406
Msa0609470 MtrunA17_Chr6g0465931 57.185 341 143 2 44 383 1 339 3.11e-139 407
Msa0609470 MtrunA17_Chr4g0008621 46.539 419 212 6 8 416 2 418 6.21e-134 397
Msa0609470 MtrunA17_Chr4g0023911 37.906 554 337 5 7 559 31 578 4.56e-125 379
Msa0609470 MtrunA17_Chr2g0310331 38.321 548 323 9 18 561 49 585 2.25e-116 357
Msa0609470 MtrunA17_Chr2g0319071 39.181 513 260 11 51 559 22 486 5.58e-115 350
Msa0609470 MtrunA17_Chr8g0357411 36.182 550 341 5 15 562 48 589 1.74e-111 344
Msa0609470 MtrunA17_Chr2g0323131 36.653 502 303 6 41 539 55 544 1.52e-109 338
Msa0609470 MtrunA17_Chr2g0323151 37.931 464 275 5 79 539 6 459 2.30e-105 325
Msa0609470 MtrunA17_Chr5g0445511 60.793 227 89 0 158 384 2 228 6.03e-100 302
Msa0609470 MtrunA17_Chr6g0466031 50.000 260 130 0 303 562 1 260 5.56e-91 280
Msa0609470 MtrunA17_Chr4g0048911 34.151 489 308 9 58 539 60 541 7.03e-90 288
Msa0609470 MtrunA17_Chr2g0310401 37.150 428 266 2 136 561 5 431 1.25e-89 283
Msa0609470 MtrunA17_Chr4g0048921 35.368 475 300 4 72 542 82 553 9.58e-88 282
Msa0609470 MtrunA17_Chr7g0237061 48.846 260 110 2 303 562 1 237 3.57e-82 256
Msa0609470 MtrunA17_Chr2g0280581 29.480 519 350 9 50 558 6 518 5.81e-77 252
Msa0609470 MtrunA17_Chr7g0237071 59.794 194 78 0 73 266 9 202 1.83e-76 241
Msa0609470 MtrunA17_Chr2g0319081 38.393 336 187 8 230 562 109 427 2.52e-73 240
Msa0609470 MtrunA17_Chr2g0319081 40.580 138 77 4 1 136 1 135 2.05e-21 97.1
Msa0609470 MtrunA17_Chr3g0108941 29.435 496 333 8 50 535 6 494 9.35e-73 241
Msa0609470 MtrunA17_Chr3g0108981 30.407 467 312 7 79 535 6 469 2.69e-72 239
Msa0609470 MtrunA17_Chr3g0108961 30.407 467 312 7 79 535 6 469 2.69e-72 239
Msa0609470 MtrunA17_Chr3g0109001 30.040 496 330 9 50 535 6 494 1.07e-71 238
Msa0609470 MtrunA17_Chr3g0105691 29.848 526 341 13 44 552 31 545 7.19e-71 237
Msa0609470 MtrunA17_Chr3g0109021 29.659 499 327 11 50 535 6 493 1.43e-69 233
Msa0609470 MtrunA17_Chr3g0109031 29.234 496 333 10 50 535 6 493 5.16e-69 231
Msa0609470 MtrunA17_Chr3g0099031 30.382 497 326 11 50 535 6 493 9.18e-69 230
Msa0609470 MtrunA17_Chr3g0109011 30.043 466 314 7 79 535 6 468 1.10e-68 229
Msa0609470 MtrunA17_Chr3g0108991 29.459 499 328 11 50 535 6 493 8.49e-68 228
Msa0609470 MtrunA17_Chr3g0108951 29.459 499 328 11 50 535 6 493 8.49e-68 228
Msa0609470 MtrunA17_Chr6g0466021 52.683 205 91 3 7 210 2 201 3.23e-67 216
Msa0609470 MtrunA17_Chr3g0108971 30.638 470 306 10 79 535 6 468 5.55e-67 225
Msa0609470 MtrunA17_Chr6g0477111 29.072 485 330 7 79 552 6 487 7.75e-67 225
Msa0609470 MtrunA17_Chr7g0237021 51.163 172 84 0 391 562 1 172 4.81e-59 194
Msa0609470 MtrunA17_Chr7g0236941 51.163 172 84 0 391 562 1 172 4.81e-59 194
Msa0609470 MtrunA17_Chr6g0477121 29.529 403 277 5 139 535 10 411 5.96e-58 199
Msa0609470 MtrunA17_Chr5g0445501 49.419 172 87 0 391 562 1 172 8.45e-58 191
Msa0609470 MtrunA17_Chr7g0217361 36.000 300 191 1 261 559 8 307 1.76e-56 192
Msa0609470 MtrunA17_Chr6g0465881 48.256 172 89 0 391 562 1 172 9.19e-55 182
Msa0609470 MtrunA17_Chr6g0465941 48.256 172 89 0 391 562 1 172 1.05e-54 182
Msa0609470 MtrunA17_Chr6g0466121 48.256 172 89 0 391 562 1 172 1.05e-54 182
Msa0609470 MtrunA17_Chr6g0465991 48.256 172 89 0 391 562 1 172 1.05e-54 182
Msa0609470 MtrunA17_Chr6g0466091 48.256 172 89 0 391 562 1 172 1.05e-54 182
Msa0609470 MtrunA17_Chr8g0357231 34.363 259 168 2 304 561 1 258 1.27e-46 164
Msa0609470 MtrunA17_Chr7g0236921 48.101 158 78 1 409 562 1 158 5.20e-46 159
Msa0609470 MtrunA17_Chr8g0357221 38.000 250 147 3 15 263 48 290 3.02e-43 155
Msa0609470 MtrunA17_Chr7g0236931 46.104 154 80 1 409 562 1 151 3.27e-42 148
Msa0609470 MtrunA17_Chr7g0233861 50.370 135 58 1 202 336 33 158 3.14e-41 147
Msa0609470 MtrunA17_Chr2g0310121 27.756 508 324 17 79 553 278 775 2.46e-37 147
Msa0609470 MtrunA17_Chr3g0101681 28.161 522 316 20 79 558 279 783 9.58e-36 142
Msa0609470 MtrunA17_Chr7g0236981 47.934 121 60 2 22 141 14 132 6.52e-33 122
Msa0609470 MtrunA17_Chr5g0445401 58.065 93 37 2 173 263 2 94 7.66e-27 104
Msa0609470 MtrunA17_Chr3g0082711 40.517 116 52 1 447 562 1 99 3.57e-22 91.3
Msa0609470 MtrunA17_Chr5g0445521 47.959 98 48 2 7 103 2 97 4.06e-22 91.7
Msa0609470 MtrunA17_Chr7g0217351 30.412 194 129 3 74 264 83 273 4.31e-22 97.1
Msa0609470 MtrunA17_Chr7g0218001 28.296 311 178 8 65 348 335 627 9.19e-22 99.8
Msa0609470 MtrunA17_Chr7g0259881 26.241 282 180 7 65 319 218 498 3.90e-21 97.8
Msa0609470 MtrunA17_Chr2g0281651 24.825 286 204 7 239 519 43 322 3.61e-17 84.0
Msa0609470 MtrunA17_Chr2g0310411 42.241 116 58 5 21 136 57 163 5.43e-17 79.0
Msa0609470 MtrunA17_Chr7g0218041 30.290 241 143 6 124 340 1 240 1.06e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609470 AT5G23960.2 42.883 548 307 4 17 562 2 545 9.54e-157 459
Msa0609470 AT5G23960.1 42.909 550 306 5 17 562 2 547 9.51e-156 457
Msa0609470 AT3G14490.1 39.964 558 324 6 11 562 49 601 1.39e-139 417
Msa0609470 AT1G70080.1 37.410 556 338 7 11 558 54 607 8.75e-129 390
Msa0609470 AT3G14540.1 36.380 547 339 4 21 562 59 601 6.66e-126 382
Msa0609470 AT3G14520.1 36.015 547 341 4 21 562 62 604 1.10e-125 382
Msa0609470 AT1G31950.1 35.662 544 341 4 24 562 67 606 2.77e-124 378
Msa0609470 AT1G31950.3 35.596 545 341 5 24 562 67 607 5.15e-123 375
Msa0609470 AT3G32030.1 36.833 562 339 8 11 562 49 604 6.32e-122 372
Msa0609470 AT1G33750.1 34.991 563 359 4 2 562 46 603 2.39e-119 365
Msa0609470 AT5G44630.1 37.453 534 326 6 32 559 23 554 3.16e-118 361
Msa0609470 AT3G29410.1 35.531 546 343 6 21 562 63 603 4.15e-118 362
Msa0609470 AT3G29410.2 35.259 502 318 4 63 562 2 498 2.53e-110 338
Msa0609470 AT4G20200.1 35.824 522 329 4 45 562 85 604 1.18e-107 335
Msa0609470 AT4G15870.1 33.813 556 346 8 10 558 54 594 1.03e-106 332
Msa0609470 AT4G16730.1 32.510 526 339 5 48 562 19 539 2.83e-106 330
Msa0609470 AT4G20210.1 31.915 564 374 5 3 562 43 600 3.27e-106 331
Msa0609470 AT4G16730.2 33.131 495 318 4 79 562 1 493 9.46e-104 322
Msa0609470 AT4G13300.1 35.196 537 332 6 32 559 22 551 1.61e-103 323
Msa0609470 AT3G14540.2 36.645 453 279 3 21 469 59 507 2.31e-101 316
Msa0609470 AT4G20230.1 33.835 532 345 6 32 558 72 601 2.61e-101 319
Msa0609470 AT4G20230.2 33.835 532 345 6 32 558 84 613 6.16e-101 318
Msa0609470 AT3G29190.1 33.395 539 345 6 32 562 69 601 6.19e-101 318
Msa0609470 AT4G13280.2 35.272 533 337 6 32 559 22 551 2.31e-100 315
Msa0609470 AT2G23230.2 34.124 548 352 8 6 546 39 584 5.04e-100 315
Msa0609470 AT2G23230.1 33.942 548 348 9 6 546 39 579 5.26e-98 310
Msa0609470 AT1G48800.1 32.911 553 362 6 12 558 53 602 6.76e-98 310
Msa0609470 AT1G31950.2 35.794 447 278 4 24 465 67 509 2.59e-97 306
Msa0609470 AT4G16740.1 32.671 554 347 11 21 562 25 564 3.89e-97 306
Msa0609470 AT3G25820.1 31.325 581 361 12 5 562 29 594 1.90e-96 306
Msa0609470 AT3G25830.1 31.325 581 361 12 5 562 29 594 1.90e-96 306
Msa0609470 AT1G66020.1 33.522 531 347 4 32 558 66 594 8.71e-96 304
Msa0609470 AT2G24210.1 32.635 573 345 13 13 562 36 590 1.25e-95 303
Msa0609470 AT3G29110.1 32.584 534 354 5 29 558 62 593 2.49e-94 300
Msa0609470 AT1G61680.1 33.607 488 310 6 58 539 77 556 2.19e-92 294
Msa0609470 AT3G25810.1 32.253 586 352 16 5 562 29 597 1.89e-90 290
Msa0609470 AT1G61680.2 33.402 488 304 7 58 539 77 549 2.47e-90 289
Msa0609470 AT4G13280.1 33.771 533 315 7 32 559 22 521 8.81e-89 284
Msa0609470 AT1G48820.1 32.058 549 312 11 21 558 62 560 5.29e-80 262
Msa0609470 AT5G48110.1 38.484 343 206 5 221 562 1 339 2.42e-78 250
Msa0609470 AT3G25820.2 33.741 409 246 7 5 399 29 426 5.18e-72 237
Msa0609470 AT4G16740.2 35.544 377 224 7 21 387 25 392 6.79e-71 233
Msa0609470 AT1G66020.2 35.817 349 218 4 32 376 66 412 2.32e-66 222
Msa0609470 AT3G25820.4 35.838 346 197 7 5 336 29 363 6.07e-62 209
Msa0609470 AT3G25820.3 35.838 346 197 7 5 336 29 363 6.07e-62 209
Msa0609470 AT4G16740.4 37.730 326 184 7 21 336 25 341 1.11e-61 208
Msa0609470 AT4G16740.3 37.730 326 184 7 21 336 25 341 1.11e-61 208
Msa0609470 AT1G79460.1 24.213 508 342 16 78 556 276 769 1.53e-28 121
Msa0609470 AT1G61120.1 22.649 521 365 14 65 559 273 781 3.39e-26 114
Msa0609470 AT4G02780.1 30.466 279 162 8 65 314 320 595 1.10e-23 106

Find 109 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAATTGAAATCTAATT+TGG 0.157846 4_2:-56834529 None:intergenic
AGATGAAGCATTGACAATTT+TGG 0.158357 4_2:-56835492 None:intergenic
GATGATATATATGATGTTTA+TGG 0.206526 4_2:+56834846 Msa0609470:CDS
CACAATAATCATGTTGAAAA+TGG 0.211729 4_2:+56833685 Msa0609470:CDS
GAGAAATCGGAGTTGGTATT+AGG 0.224064 4_2:-56833219 None:intergenic
CAAAGTCCCTTTACTCATAT+TGG 0.261866 4_2:-56835929 None:intergenic
CAAATTACCACCCCAACATT+TGG 0.286981 4_2:+56833253 Msa0609470:CDS
AAATTACCACCCCAACATTT+GGG 0.288674 4_2:+56833254 Msa0609470:CDS
CCAAGACATATTGAATAAAA+AGG 0.295172 4_2:-56833281 None:intergenic
GTCATTCTTTGTTGATCCTT+GGG 0.305154 4_2:-56835879 None:intergenic
AGAGGCTTTGGCACTTGAAT+TGG 0.307687 4_2:-56835785 None:intergenic
GATTGATTCAATCCAACGTT+TGG 0.313958 4_2:+56833612 Msa0609470:CDS
TCAATATGTCTTGGAACCTT+TGG 0.327628 4_2:+56833290 Msa0609470:CDS
GACAAACTAGCCATTGAAAT+AGG 0.327953 4_2:+56836034 Msa0609470:three_prime_UTR
AGAAATCGGAGTTGGTATTA+GGG 0.328348 4_2:-56833218 None:intergenic
TGTCATTCTTTGTTGATCCT+TGG 0.335528 4_2:-56835880 None:intergenic
GAATAGTTGAAGCTTACTTT+TGG 0.351708 4_2:+56834742 Msa0609470:CDS
GCTCTTGGAATAGTAAATTC+TGG 0.365262 4_2:+56835386 Msa0609470:CDS
TTTGTGCTTGATTACCTCAT+TGG 0.372285 4_2:-56835546 None:intergenic
ATTCAATTGCACTTGTCTTC+AGG 0.387495 4_2:+56833734 Msa0609470:CDS
TCTTCTTATGCCTATTTCAA+TGG 0.389051 4_2:-56836044 None:intergenic
CACTTGTCTTCAGGTTACTA+AGG 0.392261 4_2:+56833743 Msa0609470:CDS
TTTGGATGTTTATGAAGAAA+TGG 0.403778 4_2:+56835065 Msa0609470:CDS
ATTGATTCAATCCAACGTTT+GGG 0.408878 4_2:+56833613 Msa0609470:CDS
CAAGAAATTGCCTAGGCTAC+TGG 0.414212 4_2:+56834443 Msa0609470:CDS
CTCAATACTCTGTTGGTAGA+AGG 0.414840 4_2:+56834787 Msa0609470:CDS
GCATTACATTTCCACTTATG+AGG 0.423418 4_2:+56834470 Msa0609470:CDS
TCCATGTCCAATATGAGTAA+AGG 0.424065 4_2:+56835922 Msa0609470:exon
GCCACACATATGAGGATTCA+TGG 0.435188 4_2:+56834303 Msa0609470:CDS
TCAAATTAGCTTCCCTCAAA+GGG 0.439126 4_2:-56833591 None:intergenic
AACAACATCCTTCGTTGGAA+TGG 0.440069 4_2:+56835424 Msa0609470:CDS
CTCCGATTTCTCTAGACGTT+CGG 0.441215 4_2:+56833230 Msa0609470:CDS
CCATGTCCAATATGAGTAAA+GGG 0.444601 4_2:+56835923 Msa0609470:exon
CCCTTTACTCATATTGGACA+TGG 0.445169 4_2:-56835923 None:intergenic
TGCCGAACGTCTAGAGAAAT+CGG 0.450185 4_2:-56833232 None:intergenic
ACTATTCTGTTGCGAGCAAA+AGG 0.456530 4_2:-56834726 None:intergenic
GCCATGTACTCCTCTATTGT+TGG 0.460245 4_2:-56835365 None:intergenic
ACAACATCCTTCGTTGGAAT+GGG 0.463666 4_2:+56835425 Msa0609470:CDS
ATTGATGTGCTTTATAAAGA+TGG 0.464933 4_2:+56835839 Msa0609470:CDS
ATAGAGGAGTACATGGCTCT+TGG 0.467532 4_2:+56835371 Msa0609470:CDS
CATACTTTACTGAGGCTCAA+TGG 0.470064 4_2:+56835324 Msa0609470:CDS
AAAATGTCACAAACCCTTTG+AGG 0.477504 4_2:+56833578 Msa0609470:CDS
AAACAACATCAGAAAGAAGT+TGG 0.478105 4_2:+56834555 Msa0609470:CDS
TGTATGAAGCCACACATATG+AGG 0.482529 4_2:+56834295 Msa0609470:CDS
ATCAAATTAGCTTCCCTCAA+AGG 0.485801 4_2:-56833592 None:intergenic
ACTCGCATAAGGAGAGGCTT+TGG 0.486941 4_2:-56835797 None:intergenic
ATCTAAAATTGCTAGACTCA+TGG 0.490115 4_2:+56835511 Msa0609470:CDS
GAGCCTCAGTAAAGTATGCC+TGG 0.491242 4_2:-56835319 None:intergenic
CAAGACAAGTCACAAATGCT+TGG 0.491285 4_2:+56835735 Msa0609470:CDS
TGCACAATAGATGAAAAGAT+TGG 0.497980 4_2:+56835295 Msa0609470:intron
CAATGTCATGCTCAAAATGA+TGG 0.500528 4_2:-56833642 None:intergenic
AACTTCAAAGAAACACTTGT+TGG 0.503713 4_2:+56834249 Msa0609470:CDS
TTCTTCATAAACATCCAAAA+CGG 0.505524 4_2:-56835061 None:intergenic
ATTGAGTGGTCATAATTTCA+AGG 0.509130 4_2:-56834368 None:intergenic
AGAGGGATCTTCCTCATAAG+TGG 0.512911 4_2:-56834481 None:intergenic
TAATGCCTTGCCAGTAGCCT+AGG 0.520942 4_2:-56834453 None:intergenic
AAAATATATACCAACAATAG+AGG 0.522821 4_2:+56835355 Msa0609470:CDS
AAATGTCACAAACCCTTTGA+GGG 0.523712 4_2:+56833579 Msa0609470:CDS
TCGGAGTTGGTATTAGGGGT+TGG 0.524425 4_2:-56833213 None:intergenic
GAAATCGGAGTTGGTATTAG+GGG 0.527233 4_2:-56833217 None:intergenic
TTTGAGCCTCAATACTCTGT+TGG 0.527980 4_2:+56834780 Msa0609470:CDS
TAGCAAAATTTCATGTATTG+TGG 0.530446 4_2:-56835033 None:intergenic
ATCATCAATTACTGAGGCCA+AGG 0.530914 4_2:-56834829 None:intergenic
ACAAATTGAGAACTCGCATA+AGG 0.532358 4_2:-56835808 None:intergenic
AAGAAGTTCAAGAATGAGCA+AGG 0.540053 4_2:+56834225 Msa0609470:CDS
ATCACAACAACATCCTTCGT+TGG 0.542322 4_2:+56835419 Msa0609470:CDS
ATAGATGAAAAGATTGGTCC+AGG 0.544707 4_2:+56835301 Msa0609470:intron
ACAAGTCACAAATGCTTGGA+AGG 0.546592 4_2:+56835739 Msa0609470:CDS
AATGACCAAGGTGATATCCT+TGG 0.547258 4_2:+56834812 Msa0609470:CDS
TTTGAGCAAGAGAGAGGGCA+TGG 0.551451 4_2:+56835644 Msa0609470:CDS
TAGGTGGGATATAAGTTGCA+TGG 0.561499 4_2:+56835002 Msa0609470:intron
AGCAAAGTTGCATCATGAGA+GGG 0.572891 4_2:-56834498 None:intergenic
AATTAGTTTGAGCAAGAGAG+AGG 0.573171 4_2:+56835638 Msa0609470:intron
GGATGATATTGTTTCCAATG+AGG 0.573519 4_2:+56835532 Msa0609470:CDS
AGACAAGTGCAATTGAATGA+AGG 0.576123 4_2:-56833729 None:intergenic
AACACTTGTTGGTGATGTTG+AGG 0.576884 4_2:+56834260 Msa0609470:CDS
ACACTTGTTGGTGATGTTGA+GGG 0.577847 4_2:+56834261 Msa0609470:CDS
TGAAGAAATGGAGCAAGAGA+TGG 0.580004 4_2:+56835077 Msa0609470:CDS
ACCAACAATAGAGGAGTACA+TGG 0.580785 4_2:+56835364 Msa0609470:CDS
ATTACCACCCCAACATTTGG+GGG 0.583067 4_2:+56833256 Msa0609470:CDS
AATTACCACCCCAACATTTG+GGG 0.585116 4_2:+56833255 Msa0609470:CDS
TCCATGAATCCTCATATGTG+TGG 0.591257 4_2:-56834304 None:intergenic
CTAGGCAATTTCTTGAAGAG+TGG 0.592350 4_2:-56834435 None:intergenic
ATTAGTTTGAGCAAGAGAGA+GGG 0.599571 4_2:+56835639 Msa0609470:intron
CTTCTACCAACAGAGTATTG+AGG 0.601314 4_2:-56834786 None:intergenic
TAGCAAAGTTGCATCATGAG+AGG 0.612571 4_2:-56834499 None:intergenic
CACTCTTCAAGAAATTGCCT+AGG 0.614274 4_2:+56834436 Msa0609470:CDS
GGGATGCATTGCTACAGAAG+AGG 0.614970 4_2:+56835445 Msa0609470:CDS
GAGCAAGAGATGGTCAAGGA+AGG 0.615004 4_2:+56835087 Msa0609470:CDS
ACGTCTAGAGAAATCGGAGT+TGG 0.629425 4_2:-56833226 None:intergenic
AATGCATCCCATTCCAACGA+AGG 0.632549 4_2:-56835432 None:intergenic
GATGGTATAATCCCAAACGT+TGG 0.633632 4_2:-56833624 None:intergenic
GGTCATAATTTCAAGGTGTG+AGG 0.634454 4_2:-56834361 None:intergenic
ACATATGAGGATTCATGGAG+AGG 0.637251 4_2:+56834308 Msa0609470:CDS
TTACCACCCCAACATTTGGG+GGG 0.640939 4_2:+56833257 Msa0609470:CDS
AAATTAATCATAGCTTAAAG+CGG 0.641455 4_2:+56834412 Msa0609470:CDS
TGAGGCCAAGGATATCACCT+TGG 0.643385 4_2:-56834817 None:intergenic
TTCAGGTTACTAAGGCAACA+AGG 0.644143 4_2:+56833751 Msa0609470:CDS
GATTGCTATACTAACTCCCA+AGG 0.653661 4_2:+56835863 Msa0609470:CDS
AAGAAAAGGACTCAATTGAG+TGG 0.658799 4_2:-56834382 None:intergenic
AATGGAGCAAGAGATGGTCA+AGG 0.660378 4_2:+56835083 Msa0609470:CDS
TTGAGAACTCGCATAAGGAG+AGG 0.660485 4_2:-56835803 None:intergenic
CATGTAGCACTCAATAGCAG+AGG 0.671786 4_2:-56835669 None:intergenic
ATATATATCATCAATTACTG+AGG 0.671981 4_2:-56834835 None:intergenic
AATTGCCTAGGCTACTGGCA+AGG 0.675740 4_2:+56834448 Msa0609470:CDS
GGTCCAGGCATACTTTACTG+AGG 0.678982 4_2:+56835316 Msa0609470:CDS
TGCTTGGAAGGATATCAATG+AGG 0.688978 4_2:+56835751 Msa0609470:CDS
TGGTAGAAGGATAATGACCA+AGG 0.706697 4_2:+56834800 Msa0609470:CDS
GAGTGCTACATGAATGAACA+TGG 0.715393 4_2:+56835680 Msa0609470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAAAATCACATAATT+CGG - chr4_2:56834006-56834025 None:intergenic 10.0%
!!! TGATTTTTTAAAATTATTAG+TGG + chr4_2:56834013-56834032 Msa0609470:intron 10.0%
!! ACAAAATTAAAATGATTAAA+TGG + chr4_2:56834148-56834167 Msa0609470:intron 10.0%
!!! AATATTGAACTATTTTTTTA+TGG + chr4_2:56834941-56834960 Msa0609470:intron 10.0%
!! TTTAATTCAAATTAATTAAG+AGG - chr4_2:56834978-56834997 None:intergenic 10.0%
!!! TTAATTTGAATTAAAATTCT+AGG + chr4_2:56834983-56835002 Msa0609470:intron 10.0%
!!! TTTGTTTTCTTTAATGAATT+AGG + chr4_2:56834670-56834689 Msa0609470:intron 15.0%
!!! AAGAATTTTTACTTCATTTT+TGG - chr4_2:56835136-56835155 None:intergenic 15.0%
!! TAGGTTATATATATTACCAA+AGG - chr4_2:56833309-56833328 None:intergenic 20.0%
!!! AGTAAAATTTTGTGACAATT+GGG - chr4_2:56833471-56833490 None:intergenic 20.0%
!!! TTAATTTTTGTAGCAAGAAA+AGG - chr4_2:56834399-56834418 None:intergenic 20.0%
!! AAATTAATCATAGCTTAAAG+CGG + chr4_2:56834412-56834431 Msa0609470:CDS 20.0%
!! AAGCAAATTGAAATCTAATT+TGG - chr4_2:56834532-56834551 None:intergenic 20.0%
!! ATATATATCATCAATTACTG+AGG - chr4_2:56834838-56834857 None:intergenic 20.0%
!! GATGATATATATGATGTTTA+TGG + chr4_2:56834846-56834865 Msa0609470:CDS 20.0%
!! AATATTGAGATGTGAAAATT+TGG - chr4_2:56834927-56834946 None:intergenic 20.0%
!! ATTTGAATTAAAATTCTAGG+TGG + chr4_2:56834986-56835005 Msa0609470:intron 20.0%
!! TTTGAATTAAAATTCTAGGT+GGG + chr4_2:56834987-56835006 Msa0609470:intron 20.0%
!!! CATATTCTTGTAAATTGTTT+TGG + chr4_2:56835266-56835285 Msa0609470:intron 20.0%
!! AAAATATATACCAACAATAG+AGG + chr4_2:56835355-56835374 Msa0609470:CDS 20.0%
!!! CTATGAAGTTTTCAATTATT+GGG + chr4_2:56835986-56836005 Msa0609470:three_prime_UTR 20.0%
! CCAAGACATATTGAATAAAA+AGG - chr4_2:56833284-56833303 None:intergenic 25.0%
!! CCTTTTTATTCAATATGTCT+TGG + chr4_2:56833281-56833300 Msa0609470:CDS 25.0%
!! GTAAAATTTTGTGACAATTG+GGG - chr4_2:56833470-56833489 None:intergenic 25.0%
!! GAGTAAAATTTTGTGACAAT+TGG - chr4_2:56833472-56833491 None:intergenic 25.0%
! CACAATAATCATGTTGAAAA+TGG + chr4_2:56833685-56833704 Msa0609470:CDS 25.0%
! AAATCTAATACAAGTAGACA+AGG - chr4_2:56833804-56833823 None:intergenic 25.0%
! CTTAACCATATATTATCATG+TGG + chr4_2:56834187-56834206 Msa0609470:intron 25.0%
!! GTTTTCTTTAATGAATTAGG+TGG + chr4_2:56834673-56834692 Msa0609470:intron 25.0%
! TAGCAAAATTTCATGTATTG+TGG - chr4_2:56835036-56835055 None:intergenic 25.0%
! TTCTTCATAAACATCCAAAA+CGG - chr4_2:56835064-56835083 None:intergenic 25.0%
! TTTGGATGTTTATGAAGAAA+TGG + chr4_2:56835065-56835084 Msa0609470:CDS 25.0%
! ATTGATGTGCTTTATAAAGA+TGG + chr4_2:56835839-56835858 Msa0609470:CDS 25.0%
!! GCTATGAAGTTTTCAATTAT+TGG + chr4_2:56835985-56836004 Msa0609470:three_prime_UTR 25.0%
TTCAGTGTAATTACAAGTGT+CGG - chr4_2:56833405-56833424 None:intergenic 30.0%
TTACTCTTACTTTAATGTGC+AGG + chr4_2:56833486-56833505 Msa0609470:intron 30.0%
TAGTGCTAAAAGAAAAAGTG+AGG + chr4_2:56833536-56833555 Msa0609470:CDS 30.0%
! GGTTTGTGACATTTTTGTTA+GGG - chr4_2:56833573-56833592 None:intergenic 30.0%
! ATTGATTCAATCCAACGTTT+GGG + chr4_2:56833613-56833632 Msa0609470:CDS 30.0%
GTTAGTGTCTGTATCATATT+CGG + chr4_2:56834045-56834064 Msa0609470:intron 30.0%
TCAATATATGCTATGAAGCA+CGG - chr4_2:56834086-56834105 None:intergenic 30.0%
TAGCATATATTGATCTCCAT+CGG + chr4_2:56834093-56834112 Msa0609470:intron 30.0%
GAGAACCACATGATAATATA+TGG - chr4_2:56834195-56834214 None:intergenic 30.0%
AACTTCAAAGAAACACTTGT+TGG + chr4_2:56834249-56834268 Msa0609470:CDS 30.0%
ATTGAGTGGTCATAATTTCA+AGG - chr4_2:56834371-56834390 None:intergenic 30.0%
AAACAACATCAGAAAGAAGT+TGG + chr4_2:56834555-56834574 Msa0609470:CDS 30.0%
TTCTTTAATGAATTAGGTGG+TGG + chr4_2:56834676-56834695 Msa0609470:intron 30.0%
! GAATAGTTGAAGCTTACTTT+TGG + chr4_2:56834742-56834761 Msa0609470:CDS 30.0%
!! TGAAGCTTACTTTTGGATAT+TGG + chr4_2:56834749-56834768 Msa0609470:CDS 30.0%
!! GAAGCTTACTTTTGGATATT+GGG + chr4_2:56834750-56834769 Msa0609470:CDS 30.0%
! TTTTCACTCAAGCAATTGAA+AGG + chr4_2:56834889-56834908 Msa0609470:CDS 30.0%
!! ATTTTGCTATAAAGCCGTTT+TGG + chr4_2:56835047-56835066 Msa0609470:CDS 30.0%
TGCACAATAGATGAAAAGAT+TGG + chr4_2:56835295-56835314 Msa0609470:intron 30.0%
!!! ATTGACAATTTTGGGGTTAT+TGG - chr4_2:56835486-56835505 None:intergenic 30.0%
!!! ATGAAGCATTGACAATTTTG+GGG - chr4_2:56835493-56835512 None:intergenic 30.0%
!!! GATGAAGCATTGACAATTTT+GGG - chr4_2:56835494-56835513 None:intergenic 30.0%
!!! AGATGAAGCATTGACAATTT+TGG - chr4_2:56835495-56835514 None:intergenic 30.0%
! ATCTAAAATTGCTAGACTCA+TGG + chr4_2:56835511-56835530 Msa0609470:CDS 30.0%
TCTTCTTATGCCTATTTCAA+TGG - chr4_2:56836047-56836066 None:intergenic 30.0%
!! AGAAATCGGAGTTGGTATTA+GGG - chr4_2:56833221-56833240 None:intergenic 35.0%
AAATTACCACCCCAACATTT+GGG + chr4_2:56833254-56833273 Msa0609470:CDS 35.0%
TCAATATGTCTTGGAACCTT+TGG + chr4_2:56833290-56833309 Msa0609470:CDS 35.0%
! GGGTTTGTGACATTTTTGTT+AGG - chr4_2:56833574-56833593 None:intergenic 35.0%
AAAATGTCACAAACCCTTTG+AGG + chr4_2:56833578-56833597 Msa0609470:CDS 35.0%
AAATGTCACAAACCCTTTGA+GGG + chr4_2:56833579-56833598 Msa0609470:CDS 35.0%
! TCAAATTAGCTTCCCTCAAA+GGG - chr4_2:56833594-56833613 None:intergenic 35.0%
! ATCAAATTAGCTTCCCTCAA+AGG - chr4_2:56833595-56833614 None:intergenic 35.0%
! GATTGATTCAATCCAACGTT+TGG + chr4_2:56833612-56833631 Msa0609470:CDS 35.0%
CAATGTCATGCTCAAAATGA+TGG - chr4_2:56833645-56833664 None:intergenic 35.0%
! CATCATTTTGAGCATGACAT+TGG + chr4_2:56833643-56833662 Msa0609470:CDS 35.0%
AGACAAGTGCAATTGAATGA+AGG - chr4_2:56833732-56833751 None:intergenic 35.0%
ATTCAATTGCACTTGTCTTC+AGG + chr4_2:56833734-56833753 Msa0609470:CDS 35.0%
! ACAAGGATATCACATTTTGC+CGG + chr4_2:56833768-56833787 Msa0609470:CDS 35.0%
TAATACAAGTAGACAAGGTG+GGG - chr4_2:56833799-56833818 None:intergenic 35.0%
CTAATACAAGTAGACAAGGT+GGG - chr4_2:56833800-56833819 None:intergenic 35.0%
TCTAATACAAGTAGACAAGG+TGG - chr4_2:56833801-56833820 None:intergenic 35.0%
! TACTCTTTTTAGATGTGTCC+TGG + chr4_2:56833928-56833947 Msa0609470:intron 35.0%
!! ATCTCCATCGGTTTAATAAG+AGG + chr4_2:56834105-56834124 Msa0609470:intron 35.0%
GAAACCTCTTATTAAACCGA+TGG - chr4_2:56834112-56834131 None:intergenic 35.0%
AAGAAGTTCAAGAATGAGCA+AGG + chr4_2:56834225-56834244 Msa0609470:CDS 35.0%
AAGAAAAGGACTCAATTGAG+TGG - chr4_2:56834385-56834404 None:intergenic 35.0%
GCATTACATTTCCACTTATG+AGG + chr4_2:56834470-56834489 Msa0609470:CDS 35.0%
! ATAGATGAAAAGATTGGTCC+AGG + chr4_2:56835301-56835320 Msa0609470:intron 35.0%
GCTCTTGGAATAGTAAATTC+TGG + chr4_2:56835386-56835405 Msa0609470:CDS 35.0%
!! CTACAGAAGAGGTTTTTCAA+TGG + chr4_2:56835456-56835475 Msa0609470:CDS 35.0%
GGATGATATTGTTTCCAATG+AGG + chr4_2:56835532-56835551 Msa0609470:CDS 35.0%
TTTGTGCTTGATTACCTCAT+TGG - chr4_2:56835549-56835568 None:intergenic 35.0%
! AATTAGTTTGAGCAAGAGAG+AGG + chr4_2:56835638-56835657 Msa0609470:intron 35.0%
! ATTAGTTTGAGCAAGAGAGA+GGG + chr4_2:56835639-56835658 Msa0609470:intron 35.0%
ACAAATTGAGAACTCGCATA+AGG - chr4_2:56835811-56835830 None:intergenic 35.0%
GTCATTCTTTGTTGATCCTT+GGG - chr4_2:56835882-56835901 None:intergenic 35.0%
TGTCATTCTTTGTTGATCCT+TGG - chr4_2:56835883-56835902 None:intergenic 35.0%
TCCATGTCCAATATGAGTAA+AGG + chr4_2:56835922-56835941 Msa0609470:exon 35.0%
CCATGTCCAATATGAGTAAA+GGG + chr4_2:56835923-56835942 Msa0609470:exon 35.0%
CAAAGTCCCTTTACTCATAT+TGG - chr4_2:56835932-56835951 None:intergenic 35.0%
GACAAACTAGCCATTGAAAT+AGG + chr4_2:56836034-56836053 Msa0609470:three_prime_UTR 35.0%
!! GAAATCGGAGTTGGTATTAG+GGG - chr4_2:56833220-56833239 None:intergenic 40.0%
!! GAGAAATCGGAGTTGGTATT+AGG - chr4_2:56833222-56833241 None:intergenic 40.0%
CAAATTACCACCCCAACATT+TGG + chr4_2:56833253-56833272 Msa0609470:CDS 40.0%
AATTACCACCCCAACATTTG+GGG + chr4_2:56833255-56833274 Msa0609470:CDS 40.0%
GATGGTATAATCCCAAACGT+TGG - chr4_2:56833627-56833646 None:intergenic 40.0%
! CACTTGTCTTCAGGTTACTA+AGG + chr4_2:56833743-56833762 Msa0609470:CDS 40.0%
! TTCAGGTTACTAAGGCAACA+AGG + chr4_2:56833751-56833770 Msa0609470:CDS 40.0%
! CAAGGATATCACATTTTGCC+GGG + chr4_2:56833769-56833788 Msa0609470:CDS 40.0%
GAGTAGTGTTCGTGTTTCAT+AGG - chr4_2:56833913-56833932 None:intergenic 40.0%
!! AACACTTGTTGGTGATGTTG+AGG + chr4_2:56834260-56834279 Msa0609470:CDS 40.0%
!! ACACTTGTTGGTGATGTTGA+GGG + chr4_2:56834261-56834280 Msa0609470:CDS 40.0%
TGTATGAAGCCACACATATG+AGG + chr4_2:56834295-56834314 Msa0609470:CDS 40.0%
TCCATGAATCCTCATATGTG+TGG - chr4_2:56834307-56834326 None:intergenic 40.0%
ACATATGAGGATTCATGGAG+AGG + chr4_2:56834308-56834327 Msa0609470:CDS 40.0%
GGTCATAATTTCAAGGTGTG+AGG - chr4_2:56834364-56834383 None:intergenic 40.0%
CTAGGCAATTTCTTGAAGAG+TGG - chr4_2:56834438-56834457 None:intergenic 40.0%
CACTCTTCAAGAAATTGCCT+AGG + chr4_2:56834436-56834455 Msa0609470:CDS 40.0%
AGCAAAGTTGCATCATGAGA+GGG - chr4_2:56834501-56834520 None:intergenic 40.0%
TAGCAAAGTTGCATCATGAG+AGG - chr4_2:56834502-56834521 None:intergenic 40.0%
ACTATTCTGTTGCGAGCAAA+AGG - chr4_2:56834729-56834748 None:intergenic 40.0%
TTTGAGCCTCAATACTCTGT+TGG + chr4_2:56834780-56834799 Msa0609470:CDS 40.0%
!! CTTCTACCAACAGAGTATTG+AGG - chr4_2:56834789-56834808 None:intergenic 40.0%
! CTCAATACTCTGTTGGTAGA+AGG + chr4_2:56834787-56834806 Msa0609470:CDS 40.0%
TGGTAGAAGGATAATGACCA+AGG + chr4_2:56834800-56834819 Msa0609470:CDS 40.0%
AATGACCAAGGTGATATCCT+TGG + chr4_2:56834812-56834831 Msa0609470:CDS 40.0%
ATCATCAATTACTGAGGCCA+AGG - chr4_2:56834832-56834851 None:intergenic 40.0%
TAGGTGGGATATAAGTTGCA+TGG + chr4_2:56835002-56835021 Msa0609470:intron 40.0%
TGAAGAAATGGAGCAAGAGA+TGG + chr4_2:56835077-56835096 Msa0609470:CDS 40.0%
CATACTTTACTGAGGCTCAA+TGG + chr4_2:56835324-56835343 Msa0609470:CDS 40.0%
! ACCAACAATAGAGGAGTACA+TGG + chr4_2:56835364-56835383 Msa0609470:CDS 40.0%
ATCACAACAACATCCTTCGT+TGG + chr4_2:56835419-56835438 Msa0609470:CDS 40.0%
AACAACATCCTTCGTTGGAA+TGG + chr4_2:56835424-56835443 Msa0609470:CDS 40.0%
ACAACATCCTTCGTTGGAAT+GGG + chr4_2:56835425-56835444 Msa0609470:CDS 40.0%
GAGTGCTACATGAATGAACA+TGG + chr4_2:56835680-56835699 Msa0609470:CDS 40.0%
CAAGACAAGTCACAAATGCT+TGG + chr4_2:56835735-56835754 Msa0609470:CDS 40.0%
ACAAGTCACAAATGCTTGGA+AGG + chr4_2:56835739-56835758 Msa0609470:CDS 40.0%
TGCTTGGAAGGATATCAATG+AGG + chr4_2:56835751-56835770 Msa0609470:CDS 40.0%
GATTGCTATACTAACTCCCA+AGG + chr4_2:56835863-56835882 Msa0609470:CDS 40.0%
CCCTTTACTCATATTGGACA+TGG - chr4_2:56835926-56835945 None:intergenic 40.0%
ACGTCTAGAGAAATCGGAGT+TGG - chr4_2:56833229-56833248 None:intergenic 45.0%
CTCCGATTTCTCTAGACGTT+CGG + chr4_2:56833230-56833249 Msa0609470:CDS 45.0%
TGCCGAACGTCTAGAGAAAT+CGG - chr4_2:56833235-56833254 None:intergenic 45.0%
ATTACCACCCCAACATTTGG+GGG + chr4_2:56833256-56833275 Msa0609470:CDS 45.0%
AAAAGGTCCCCCCAAATGTT+GGG - chr4_2:56833267-56833286 None:intergenic 45.0%
AAAAAGGTCCCCCCAAATGT+TGG - chr4_2:56833268-56833287 None:intergenic 45.0%
TATGAAGCACAGACACTCCT+CGG + chr4_2:56833327-56833346 Msa0609470:intron 45.0%
ATGAAGCACGGATACAGACA+TGG - chr4_2:56834074-56834093 None:intergenic 45.0%
GCCACACATATGAGGATTCA+TGG + chr4_2:56834303-56834322 Msa0609470:CDS 45.0%
CAAGAAATTGCCTAGGCTAC+TGG + chr4_2:56834443-56834462 Msa0609470:CDS 45.0%
AGAGGGATCTTCCTCATAAG+TGG - chr4_2:56834484-56834503 None:intergenic 45.0%
! GTGTCTGAAACGTCTCGAAT+AGG - chr4_2:56834628-56834647 None:intergenic 45.0%
AATGGAGCAAGAGATGGTCA+AGG + chr4_2:56835083-56835102 Msa0609470:CDS 45.0%
GCCATGTACTCCTCTATTGT+TGG - chr4_2:56835368-56835387 None:intergenic 45.0%
! ATAGAGGAGTACATGGCTCT+TGG + chr4_2:56835371-56835390 Msa0609470:CDS 45.0%
AATGCATCCCATTCCAACGA+AGG - chr4_2:56835435-56835454 None:intergenic 45.0%
CATGTAGCACTCAATAGCAG+AGG - chr4_2:56835672-56835691 None:intergenic 45.0%
! AGAGGCTTTGGCACTTGAAT+TGG - chr4_2:56835788-56835807 None:intergenic 45.0%
TTGAGAACTCGCATAAGGAG+AGG - chr4_2:56835806-56835825 None:intergenic 45.0%
!! ATAAAAAAAATAAAAAAACA+AGG - chr4_2:56835172-56835191 None:intergenic 5.0%
!! AAAATATAAATTTAAAAGAT+AGG - chr4_2:56835589-56835608 None:intergenic 5.0%
!! TCGGAGTTGGTATTAGGGGT+TGG - chr4_2:56833216-56833235 None:intergenic 50.0%
TTACCACCCCAACATTTGGG+GGG + chr4_2:56833257-56833276 Msa0609470:CDS 50.0%
AAAGGTCCCCCCAAATGTTG+GGG - chr4_2:56833266-56833285 None:intergenic 50.0%
!! GAGGAGTGTCTGTGCTTCAT+AGG - chr4_2:56833328-56833347 None:intergenic 50.0%
!! TACAAGTGTCGGTGTCGTGT+TGG - chr4_2:56833394-56833413 None:intergenic 50.0%
TAGACAAGGTGGGGTTTACC+CGG - chr4_2:56833790-56833809 None:intergenic 50.0%
ATCATGTGTCAGAGTCGCGT+CGG - chr4_2:56833979-56833998 None:intergenic 50.0%
AATTGCCTAGGCTACTGGCA+AGG + chr4_2:56834448-56834467 Msa0609470:CDS 50.0%
TAATGCCTTGCCAGTAGCCT+AGG - chr4_2:56834456-56834475 None:intergenic 50.0%
TGAGGCCAAGGATATCACCT+TGG - chr4_2:56834820-56834839 None:intergenic 50.0%
GAGCAAGAGATGGTCAAGGA+AGG + chr4_2:56835087-56835106 Msa0609470:CDS 50.0%
GGTCCAGGCATACTTTACTG+AGG + chr4_2:56835316-56835335 Msa0609470:CDS 50.0%
GAGCCTCAGTAAAGTATGCC+TGG - chr4_2:56835322-56835341 None:intergenic 50.0%
GGGATGCATTGCTACAGAAG+AGG + chr4_2:56835445-56835464 Msa0609470:CDS 50.0%
TTTGAGCAAGAGAGAGGGCA+TGG + chr4_2:56835644-56835663 Msa0609470:CDS 50.0%
ACTCGCATAAGGAGAGGCTT+TGG - chr4_2:56835800-56835819 None:intergenic 50.0%
GCACAGACACTCCTCGGATT+AGG + chr4_2:56833333-56833352 Msa0609470:intron 55.0%
!! TGTCGGTGTCGTGTTGGTGT+CGG - chr4_2:56833388-56833407 None:intergenic 55.0%
AACGACACGTGTTCGACACC+AGG - chr4_2:56833949-56833968 None:intergenic 55.0%
! TCCTCGGATTAGGCGTGTCC+CGG + chr4_2:56833343-56833362 Msa0609470:intron 60.0%
GATTAGGCGTGTCCCGGTGT+CGG + chr4_2:56833349-56833368 Msa0609470:intron 60.0%
ACACGACACGTGTCCGACAC+CGG - chr4_2:56833365-56833384 None:intergenic 60.0%
! TGTCAGAGTCGCGTCGGTGT+TGG - chr4_2:56833973-56833992 None:intergenic 60.0%
GGTCCCCCCAAATGTTGGGG+TGG - chr4_2:56833263-56833282 None:intergenic 65.0%
ACCGGGACACGCCTAATCCG+AGG - chr4_2:56833347-56833366 None:intergenic 65.0%
CACGACACGTGTCCGACACC+GGG - chr4_2:56833364-56833383 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 56833180 56836079 56833180 ID=Msa0609470;Name=Msa0609470
chr4_2 mRNA 56833180 56836079 56833180 ID=Msa0609470-mRNA-1;Parent=Msa0609470;Name=Msa0609470-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.66|0.57|1|1|1|7|142|562
chr4_2 exon 56833180 56833311 56833180 ID=Msa0609470-mRNA-1:exon:14722;Parent=Msa0609470-mRNA-1
chr4_2 exon 56833508 56833790 56833508 ID=Msa0609470-mRNA-1:exon:14723;Parent=Msa0609470-mRNA-1
chr4_2 exon 56834217 56834589 56834217 ID=Msa0609470-mRNA-1:exon:14724;Parent=Msa0609470-mRNA-1
chr4_2 exon 56834692 56834910 56834692 ID=Msa0609470-mRNA-1:exon:14725;Parent=Msa0609470-mRNA-1
chr4_2 exon 56835005 56835143 56835005 ID=Msa0609470-mRNA-1:exon:14726;Parent=Msa0609470-mRNA-1
chr4_2 exon 56835305 56835553 56835305 ID=Msa0609470-mRNA-1:exon:14727;Parent=Msa0609470-mRNA-1
chr4_2 exon 56835644 56836079 56835644 ID=Msa0609470-mRNA-1:exon:14728;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56833180 56833311 56833180 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56833508 56833790 56833508 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56834217 56834589 56834217 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56834692 56834910 56834692 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56835005 56835143 56835005 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56835305 56835553 56835305 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 CDS 56835644 56835937 56835644 ID=Msa0609470-mRNA-1:cds;Parent=Msa0609470-mRNA-1
chr4_2 three_prime_UTR 56835938 56836079 56835938 ID=Msa0609470-mRNA-1:three_prime_utr;Parent=Msa0609470-mRNA-1
Gene Sequence

>Msa0609470

ATGTCGGTTTCAGTAACATCATCACTAGTTGCTCCAACCCCTAATACCAACTCCGATTTCTCTAGACGTTCGGCAAATTACCACCCCAACATTTGGGGGGACCTTTTTATTCAATATGTCTTGGAACCTTTGGAATTTGATGAAATAATGAAGCAGATTATAGTGCTAAAAGAAAAAGTGAGGCAGATGCTAGTCCCTAACAAAAATGTCACAAACCCTTTGAGGGAAGCTAATTTGATTGATTCAATCCAACGTTTGGGATTATACCATCATTTTGAGCATGACATTGGAGAATTATTGCGACATATTCACAATAATCATGTTGAAAATGGAACAATAACTCTTAACGAAGACCTTCATTCAATTGCACTTGTCTTCAGGTTACTAAGGCAACAAGGATATCACATTTTGCCGGATGCTTTTAAGAAGTTCAAGAATGAGCAAGGAAACTTCAAAGAAACACTTGTTGGTGATGTTGAGGGAATGCTAAGTTTGTATGAAGCCACACATATGAGGATTCATGGAGAGGATATATTAGATGAAGCACTTTCTTTCACTTCCTCACACCTTGAAATTATGACCACTCAATTGAGTCCTTTTCTTGCTACAAAAATTAATCATAGCTTAAAGCGGCCACTCTTCAAGAAATTGCCTAGGCTACTGGCAAGGCATTACATTTCCACTTATGAGGAAGATCCCTCTCATGATGCAACTTTGCTATTACTTGCCAAATTAGATTTCAATTTGCTTCAAAAACAACATCAGAAAGAAGTTGGCGATATTTCAAAGTGGTGGAAAGATTTAGATTTTGCAACAAAACTACCTTTTGCTCGCAACAGAATAGTTGAAGCTTACTTTTGGATATTGGGAGTGTATTTTGAGCCTCAATACTCTGTTGGTAGAAGGATAATGACCAAGGTGATATCCTTGGCCTCAGTAATTGATGATATATATGATGTTTATGGTACAATAGAAGAGCTACAACTTTTCACTCAAGCAATTGAAAGGTGGGATATAAGTTGCATGGATTTTCTACCACAATACATGAAATTTTGCTATAAAGCCGTTTTGGATGTTTATGAAGAAATGGAGCAAGAGATGGTCAAGGAAGGCAGAGCATTTTGTGTAACATATGCCAAAAATGAAATGAAAAGATTGGTCCAGGCATACTTTACTGAGGCTCAATGGTTCAGTAGAAAATATATACCAACAATAGAGGAGTACATGGCTCTTGGAATAGTAAATTCTGGTTACTATCTAATCACAACAACATCCTTCGTTGGAATGGGATGCATTGCTACAGAAGAGGTTTTTCAATGGTTAACCAATAACCCCAAAATTGTCAATGCTTCATCTAAAATTGCTAGACTCATGGATGATATTGTTTCCAATGAGTTTGAGCAAGAGAGAGGGCATGGTGCCTCTGCTATTGAGTGCTACATGAATGAACATGGTGTGTCTAGAGAAGATGCAGTTAATGAACTTTCAAGACAAGTCACAAATGCTTGGAAGGATATCAATGAGGAACTTCTTGATCCAATTCAAGTGCCAAAGCCTCTCCTTATGCGAGTTCTCAATTTGTCACGTGTCATTGATGTGCTTTATAAAGATGGAGATTGCTATACTAACTCCCAAGGATCAACAAAGAATGACATAATATCTATCTTGTTGAATCCATGTCCAATATGA
Protein sequence

>Msa0609470

MSVSVTSSLVAPTPNTNSDFSRRSANYHPNIWGDLFIQYVLEPLEFDEIMKQIIVLKEKVRQMLVPNKNVTNPLREANLIDSIQRLGLYHHFEHDIGELLRHIHNNHVENGTITLNEDLHSIALVFRLLRQQGYHILPDAFKKFKNEQGNFKETLVGDVEGMLSLYEATHMRIHGEDILDEALSFTSSHLEIMTTQLSPFLATKINHSLKRPLFKKLPRLLARHYISTYEEDPSHDATLLLLAKLDFNLLQKQHQKEVGDISKWWKDLDFATKLPFARNRIVEAYFWILGVYFEPQYSVGRRIMTKVISLASVIDDIYDVYGTIEELQLFTQAIERWDISCMDFLPQYMKFCYKAVLDVYEEMEQEMVKEGRAFCVTYAKNEMKRLVQAYFTEAQWFSRKYIPTIEEYMALGIVNSGYYLITTTSFVGMGCIATEEVFQWLTNNPKIVNASSKIARLMDDIVSNEFEQERGHGASAIECYMNEHGVSREDAVNELSRQVTNAWKDINEELLDPIQVPKPLLMRVLNLSRVIDVLYKDGDCYTNSQGSTKNDIISILLNPCPI*