AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1432730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432730 MtrunA17_Chr7g0273731 90.948 464 23 1 1 464 84 528 0.0 879
Msa1432730 MtrunA17_Chr7g0273791 80.388 464 72 1 1 464 1 445 0.0 788
Msa1432730 MtrunA17_Chr7g0273781 78.448 464 81 1 1 464 34 478 0.0 776
Msa1432730 MtrunA17_Chr5g0433641 74.624 465 98 2 1 464 1 446 0.0 729
Msa1432730 MtrunA17_Chr7g0273761 74.409 465 98 3 2 464 374 819 0.0 724
Msa1432730 MtrunA17_Chr7g0273761 74.000 300 77 1 166 464 32 331 8.49e-162 478
Msa1432730 MtrunA17_Chr7g0273721 73.118 465 104 3 2 464 3 448 0.0 718
Msa1432730 MtrunA17_Chr1g0165671 56.332 458 173 6 6 460 23 456 0.0 533
Msa1432730 MtrunA17_Chr1g0165661 52.079 457 196 5 6 460 5 440 9.91e-170 485
Msa1432730 MtrunA17_Chr1g0191081 53.879 464 190 5 3 464 11 452 3.15e-169 483
Msa1432730 MtrunA17_Chr1g0165681 52.183 458 194 6 6 460 5 440 9.83e-169 483
Msa1432730 MtrunA17_Chr1g0165711 47.495 459 176 7 6 463 5 399 5.22e-142 413
Msa1432730 MtrunA17_Chr7g0273741 82.993 147 25 0 1 147 1 147 1.61e-84 256
Msa1432730 MtrunA17_Chr6g0471381 54.435 248 105 5 217 460 6 249 4.59e-84 259
Msa1432730 MtrunA17_Chr7g0273701 72.500 160 44 0 305 464 1 160 7.96e-82 250
Msa1432730 MtrunA17_Chr3g0098961 76.871 147 34 0 1 147 4 150 7.06e-78 240
Msa1432730 MtrunA17_Chr3g0132001 32.143 448 266 10 10 452 14 428 1.45e-68 225
Msa1432730 MtrunA17_Chr8g0386141 32.353 408 242 10 8 409 5 384 9.29e-68 223
Msa1432730 MtrunA17_Chr7g0224391 31.514 403 246 7 13 411 19 395 1.57e-65 217
Msa1432730 MtrunA17_Chr6g0471971 47.257 237 99 5 181 414 1 214 1.84e-65 210
Msa1432730 MtrunA17_Chr6g0471371 63.087 149 55 0 6 154 5 153 3.05e-65 207
Msa1432730 MtrunA17_Chr1g0165741 63.380 142 52 0 6 147 5 146 2.06e-62 199
Msa1432730 MtrunA17_Chr8g0353421 62.992 127 47 0 3 129 2 128 1.24e-57 186
Msa1432730 MtrunA17_Chr3g0141851 30.583 412 238 13 18 415 20 397 2.80e-51 180
Msa1432730 MtrunA17_Chr2g0306321 29.158 463 285 15 8 456 24 457 2.62e-48 172
Msa1432730 MtrunA17_Chr7g0273691 73.196 97 25 1 185 280 1 97 4.04e-46 155
Msa1432730 MtrunA17_Chr4g0027071 27.682 466 281 15 17 457 19 453 6.87e-43 157
Msa1432730 MtrunA17_Chr6g0471891 44.444 198 81 5 251 445 1 172 1.71e-42 150
Msa1432730 MtrunA17_Chr2g0334001 27.511 458 288 13 6 452 1 425 2.51e-42 155
Msa1432730 MtrunA17_Chr4g0001861 27.945 433 250 12 42 452 34 426 1.55e-41 153
Msa1432730 MtrunA17_Chr3g0113821 27.802 464 285 14 9 455 4 434 1.95e-39 147
Msa1432730 MtrunA17_Chr8g0371821 26.407 462 292 14 6 456 1 425 7.11e-38 143
Msa1432730 MtrunA17_Chr7g0270771 27.796 313 175 7 6 310 1 270 1.01e-37 139
Msa1432730 MtrunA17_Chr2g0283361 27.477 444 271 13 31 453 25 438 1.15e-32 129
Msa1432730 MtrunA17_Chr7g0253501 26.931 479 273 20 17 454 9 451 6.53e-32 127
Msa1432730 MtrunA17_Chr7g0253531 26.752 471 268 19 17 447 9 442 1.43e-31 126
Msa1432730 MtrunA17_Chr4g0072041 25.446 448 273 14 36 454 39 454 8.97e-31 124
Msa1432730 MtrunA17_Chr3g0087401 25.000 428 261 12 18 413 10 409 1.77e-30 122
Msa1432730 MtrunA17_Chr3g0106691 27.488 422 264 13 4 408 52 448 2.80e-29 120
Msa1432730 MtrunA17_Chr7g0273681 92.308 52 4 0 92 143 1 52 3.49e-29 108
Msa1432730 MtrunA17_Chr6g0470591 25.822 426 261 16 60 459 66 462 1.14e-27 115
Msa1432730 MtrunA17_Chr1g0165721 57.292 96 40 1 366 460 1 96 1.17e-27 106
Msa1432730 MtrunA17_Chr4g0054081 24.000 450 278 14 36 454 34 450 1.84e-27 114
Msa1432730 MtrunA17_Chr7g0253541 25.407 492 275 22 18 460 12 460 2.11e-27 114
Msa1432730 MtrunA17_Chr2g0311401 28.000 300 197 8 166 454 6 297 2.42e-27 111
Msa1432730 MtrunA17_Chr7g0252731 26.014 419 252 14 3 409 8 380 1.32e-26 111
Msa1432730 MtrunA17_Chr1g0165731 42.202 109 61 2 181 288 1 108 5.01e-25 99.4
Msa1432730 MtrunA17_Chr5g0442691 23.093 472 294 16 8 444 31 468 5.60e-25 107
Msa1432730 MtrunA17_Chr5g0442701 23.517 472 292 16 8 444 8 445 3.21e-24 105
Msa1432730 MtrunA17_Chr3g0106711 31.081 222 122 6 4 217 6 204 1.46e-23 99.0
Msa1432730 MtrunA17_Chr3g0106661 26.195 481 284 17 17 459 17 464 4.49e-23 101
Msa1432730 MtrunA17_Chr3g0106721 26.462 359 220 10 17 358 17 348 1.50e-22 100
Msa1432730 MtrunA17_Chr5g0442721 22.912 419 258 12 62 447 64 450 2.71e-22 99.4
Msa1432730 MtrunA17_Chr8g0354371 22.796 465 272 17 24 459 31 437 2.09e-20 93.6
Msa1432730 MtrunA17_Chr3g0095731 23.009 452 280 16 43 460 43 460 8.01e-20 91.7
Msa1432730 MtrunA17_Chr7g0235531 23.246 456 299 15 17 454 11 433 2.24e-19 90.1
Msa1432730 MtrunA17_Chr3g0095721 23.239 426 264 13 65 460 72 464 4.37e-19 89.7
Msa1432730 MtrunA17_Chr7g0273671 88.372 43 5 0 1 43 1 43 6.78e-19 80.1
Msa1432730 MtrunA17_Chr3g0123061 22.101 457 283 18 16 457 11 409 8.20e-19 88.2
Msa1432730 MtrunA17_Chr4g0004691 28.108 185 108 3 20 204 14 173 1.98e-18 83.2
Msa1432730 MtrunA17_Chr3g0134091 26.940 464 260 22 24 458 30 443 2.19e-18 87.4
Msa1432730 MtrunA17_Chr3g0134061 26.464 427 250 19 37 438 42 429 6.29e-18 85.9
Msa1432730 MtrunA17_Chr3g0134121 26.444 450 263 22 25 449 31 437 9.29e-18 85.5
Msa1432730 MtrunA17_Chr3g0106761 25.613 367 227 13 60 408 9 347 1.13e-17 84.7
Msa1432730 MtrunA17_Chr4g0010331 23.438 448 282 17 24 455 25 427 4.01e-17 83.6
Msa1432730 MtrunA17_Chr6g0471981 64.706 51 18 0 101 151 1 51 4.40e-17 75.5
Msa1432730 MtrunA17_Chr3g0106741 29.297 256 164 9 166 408 127 378 1.66e-16 81.6
Msa1432730 MtrunA17_Chr8g0376911 22.222 486 264 21 16 457 9 424 2.56e-16 80.9
Msa1432730 MtrunA17_Chr4g0010361 25.721 416 245 20 24 416 25 399 5.25e-16 80.1
Msa1432730 MtrunA17_Chr4g0010311 24.508 457 272 20 24 455 25 433 5.37e-16 80.1
Msa1432730 MtrunA17_Chr8g0374641 22.010 418 257 15 24 418 30 401 9.18e-16 79.3
Msa1432730 MtrunA17_Chr1g0199561 22.146 438 279 15 16 427 9 410 2.00e-15 78.2
Msa1432730 MtrunA17_Chr8g0376901 23.256 473 274 19 16 456 9 424 6.13e-15 76.6
Msa1432730 MtrunA17_Chr3g0134071 25.110 454 263 21 37 460 44 450 1.36e-14 75.9
Msa1432730 MtrunA17_Chr5g0414431 55.357 56 20 1 52 107 2 52 4.24e-14 66.6
Msa1432730 MtrunA17_Chr7g0224131 22.819 447 289 15 24 455 25 430 4.57e-14 73.9
Msa1432730 MtrunA17_Chr4g0053111 21.861 462 282 17 24 455 28 440 8.63e-14 73.2
Msa1432730 MtrunA17_Chr4g0010351 25.392 319 189 15 24 328 25 308 4.17e-13 70.9
Msa1432730 MtrunA17_Chr4g0053081 21.459 466 282 18 24 460 33 443 5.47e-13 70.9
Msa1432730 MtrunA17_Chr3g0106631 29.286 140 94 3 167 302 2 140 1.59e-11 62.8
Msa1432730 MtrunA17_Chr2g0311411 31.532 111 72 3 6 116 1 107 7.55e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432730 AT5G17540.1 47.210 466 217 9 3 463 2 443 1.25e-141 414
Msa1432730 AT3G03480.1 45.376 465 224 9 2 461 11 450 9.63e-136 399
Msa1432730 AT3G48720.1 32.353 408 246 10 8 411 7 388 1.42e-69 228
Msa1432730 AT5G63560.1 33.907 407 233 10 10 411 9 384 6.36e-65 215
Msa1432730 AT5G41040.1 31.863 408 246 8 10 411 33 414 9.92e-65 216
Msa1432730 AT5G41040.2 31.863 408 246 8 10 411 17 398 1.37e-64 215
Msa1432730 AT1G27620.1 30.751 413 243 12 13 415 12 391 5.46e-52 182
Msa1432730 AT1G03390.1 30.222 450 270 15 8 444 23 441 1.26e-47 171
Msa1432730 AT5G48930.1 27.451 459 282 14 17 459 10 433 1.66e-42 156
Msa1432730 AT2G19070.1 28.698 453 261 15 36 458 29 449 2.51e-41 153
Msa1432730 AT2G40230.1 29.268 410 246 12 17 412 12 391 5.82e-39 146
Msa1432730 AT3G62160.1 26.316 418 250 12 6 409 1 374 3.46e-37 141
Msa1432730 AT1G28680.1 26.697 442 286 11 31 459 27 443 2.61e-35 137
Msa1432730 AT5G57840.1 27.101 476 284 15 10 461 3 439 5.35e-35 135
Msa1432730 AT1G28680.2 28.653 349 217 9 31 372 27 350 3.25e-32 127
Msa1432730 AT1G65450.1 26.526 426 265 13 3 410 8 403 3.85e-31 125
Msa1432730 AT5G07080.1 25.527 427 267 11 3 408 7 403 1.38e-30 123
Msa1432730 AT3G47170.1 24.280 486 306 17 8 460 10 466 1.42e-28 118
Msa1432730 AT2G25150.1 27.934 426 253 17 5 408 7 400 3.35e-27 114
Msa1432730 AT2G23510.1 26.066 422 267 16 2 408 7 398 2.05e-25 108
Msa1432730 AT1G32910.1 24.103 390 236 12 63 428 63 416 3.49e-21 96.3
Msa1432730 AT3G50280.1 25.537 419 247 17 24 418 28 405 2.79e-20 93.6
Msa1432730 AT1G78990.1 22.779 439 280 12 43 460 45 445 1.90e-19 90.9
Msa1432730 AT3G26040.1 23.333 420 253 16 16 411 9 383 4.74e-19 89.7
Msa1432730 AT4G31910.1 24.454 458 286 18 26 454 27 453 2.48e-18 87.8
Msa1432730 AT1G24430.1 22.989 435 262 15 44 460 41 420 2.58e-18 87.4
Msa1432730 AT5G07850.1 25.732 478 278 20 14 459 17 449 1.37e-17 85.5
Msa1432730 AT5G16410.1 22.554 368 228 12 65 411 91 422 3.26e-17 84.3
Msa1432730 AT5G07860.1 24.678 466 290 18 14 458 17 442 5.71e-17 83.6
Msa1432730 AT3G50270.1 23.227 409 257 13 36 418 33 410 4.80e-16 80.5
Msa1432730 AT5G47980.1 24.784 464 277 21 14 452 9 425 3.63e-15 77.8
Msa1432730 AT1G24420.2 22.500 400 211 12 76 441 76 410 2.50e-14 75.1
Msa1432730 AT1G24420.1 22.500 400 211 12 76 441 76 410 2.50e-14 75.1
Msa1432730 AT5G07870.1 23.517 472 304 19 14 459 17 457 1.83e-12 69.7
Msa1432730 AT1G65450.3 24.068 295 199 9 179 460 3 285 1.83e-12 68.2
Msa1432730 AT5G47950.1 24.382 283 168 9 16 294 12 252 1.24e-11 67.0

Find 135 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATAAAGATTGATGTATT+TGG 0.213067 tig0027169:+10515 None:intergenic
GCATGTCTAAGAACTTTAAT+AGG 0.218647 tig0027169:+13520 None:intergenic
AGCAGATCTTATGGTTATTA+AGG 0.242062 tig0027169:-10963 Msa1432730:CDS
AAGTTCAAAGAAGTGGATTT+TGG 0.248604 tig0027169:-10884 Msa1432730:CDS
ATGTAACACTTGATCAATTT+GGG 0.256344 tig0027169:-13378 Msa1432730:CDS
ACTTGACAAGATTTAGTTAC+AGG 0.266265 tig0027169:+14039 None:intergenic
ATCCCACATAAGAATGTAAA+AGG 0.288527 tig0027169:-10791 Msa1432730:CDS
GATGTAACACTTGATCAATT+TGG 0.298925 tig0027169:-13379 Msa1432730:CDS
TGCTTGGCAACGACACAATA+AGG 0.312592 tig0027169:-13861 Msa1432730:five_prime_UTR
AAACTGGCACATGAAATTTA+AGG 0.316301 tig0027169:+13926 None:intergenic
ATCCATTTGCAGGAAGGATT+AGG 0.320371 tig0027169:-13471 Msa1432730:CDS
ATGAATATTATTGGGAAAAC+TGG 0.323391 tig0027169:+13910 None:intergenic
CCATTCAACCTGTATGGAAT+AGG 0.323735 tig0027169:-11444 Msa1432730:CDS
GCATTCTGTAGCAGATCTTA+TGG 0.324794 tig0027169:-10972 Msa1432730:CDS
TCCATTTGCAGGAAGGATTA+GGG 0.331515 tig0027169:-13470 Msa1432730:CDS
TCGCCACTTGTTGGATATTA+CGG 0.334686 tig0027169:-11112 Msa1432730:CDS
TTTGGGTGATGAATATTATT+GGG 0.337019 tig0027169:+13902 None:intergenic
CATAGCTGCGCTTCGTCTCT+TGG 0.337255 tig0027169:-11284 Msa1432730:CDS
AAGCTTTGTGGAAACTCATT+AGG 0.345233 tig0027169:-11049 Msa1432730:CDS
AACTAGGAACCATTTGCTTT+AGG 0.348486 tig0027169:+10674 None:intergenic
CCCGCGGTGGTGACAGCTGC+CGG 0.349688 tig0027169:+11073 None:intergenic
GCAGCTGGTGGCACCAGCTC+TGG 0.355758 tig0027169:+13658 None:intergenic
GTGTATTACTATCCATTTGC+AGG 0.355829 tig0027169:-13481 Msa1432730:CDS
CACAAAAGCATTGCAGTTAG+AGG 0.365161 tig0027169:-11197 Msa1432730:CDS
ATTCTTATGTGGGATGATAT+AGG 0.365851 tig0027169:+10799 None:intergenic
GGATGGGGTGAGGCGGTGTA+TGG 0.375232 tig0027169:-10863 Msa1432730:CDS
GGGTGCTGGGCGTAAGTTGA+TGG 0.381264 tig0027169:-13446 Msa1432730:CDS
CATGAATGCCTGGGCAGAAA+TGG 0.382624 tig0027169:-11488 Msa1432730:CDS
CTCCGCACTGTGAACAATAA+TGG 0.390252 tig0027169:+13688 None:intergenic
GGATCACAAATAATTATTTA+CGG 0.390810 tig0027169:+10461 None:intergenic
CATGTCTAAGAACTTTAATA+GGG 0.395837 tig0027169:+13521 None:intergenic
CATTCAACCTGTATGGAATA+GGG 0.408225 tig0027169:-11443 Msa1432730:CDS
TATTCATGAACACGTGGAAT+AGG 0.410021 tig0027169:+13977 None:intergenic
TCCCTAATCCTTCCTGCAAA+TGG 0.413944 tig0027169:+13469 None:intergenic
GTTCTTTGAAGCATGGGAAT+GGG 0.414516 tig0027169:+13341 None:intergenic
AACCTTCCATTCAACCTGTA+TGG 0.427456 tig0027169:-11450 Msa1432730:CDS
GGAAGGATTAGGGAGGGTGC+TGG 0.433324 tig0027169:-13460 Msa1432730:CDS
CGTAGTATCTTCTTCCTTGA+CGG 0.435537 tig0027169:+11345 None:intergenic
TGAAACTATTCATGAATGCC+TGG 0.438374 tig0027169:-11498 Msa1432730:CDS
TCCCTTCACCACACCGGTCA+AGG 0.439345 tig0027169:-12004 Msa1432730:CDS
GAACTTCTATACGATGTTCC+AGG 0.439564 tig0027169:-13322 Msa1432730:CDS
TTTACCTGTATGAGTCGAAT+GGG 0.448569 tig0027169:+13277 None:intergenic
CAGCTCCATCGCTCATTGTA+TGG 0.451440 tig0027169:+11524 None:intergenic
CAAAATTAACCTAAAGCAAA+TGG 0.452519 tig0027169:-10683 Msa1432730:exon
CCTATTCCATACAGGTTGAA+TGG 0.453015 tig0027169:+11444 None:intergenic
GTTTATATCACATGCTTACA+TGG 0.453078 tig0027169:-13830 Msa1432730:five_prime_UTR
ACCACCGCGGGGAAGCTTTG+TGG 0.458585 tig0027169:-11061 Msa1432730:CDS
CTGGTGGCACCAGCTCTGGT+TGG 0.466144 tig0027169:+13662 None:intergenic
TGACGATAGATAAACATCAT+AGG 0.470860 tig0027169:+13568 None:intergenic
AAGAATGTAAAAGGAGAAGA+AGG 0.472789 tig0027169:-10782 Msa1432730:CDS
GCCCTTGACCGGTGTGGTGA+AGG 0.473028 tig0027169:+12002 None:intergenic
TCCTCGAGCCATTTCTGCCC+AGG 0.474546 tig0027169:+11480 None:intergenic
AGAATCTCCCTATTCCATAC+AGG 0.480630 tig0027169:+11436 None:intergenic
TCAATAATTAGTTCTGATCC+TGG 0.483822 tig0027169:+13304 None:intergenic
TTCCATACAGGTTGAATGGA+AGG 0.484244 tig0027169:+11448 None:intergenic
GTCAACCATTCGCCACTTGT+TGG 0.484423 tig0027169:-11121 Msa1432730:CDS
AGTTCTTTGAAGCATGGGAA+TGG 0.487665 tig0027169:+13340 None:intergenic
ATATAGGCTGCTCCAGGAAA+AGG 0.487690 tig0027169:+10815 None:intergenic
TGGGGTGAGGCGGTGTATGG+TGG 0.491453 tig0027169:-10860 Msa1432730:CDS
GCGGAGGAGCCAACCAGAGC+TGG 0.493190 tig0027169:-13671 Msa1432730:CDS
CCGCCCCATTCGACTCATAC+AGG 0.494546 tig0027169:-13281 Msa1432730:intron
AAATATGTTCGTATTCATGA+TGG 0.495591 tig0027169:+11380 None:intergenic
CCCTTGACCGGTGTGGTGAA+GGG 0.498111 tig0027169:+12003 None:intergenic
CCGGCAGCTGTCACCACCGC+GGG 0.498263 tig0027169:-11073 Msa1432730:CDS
ATGGAATAGGGAGATTCTAA+TGG 0.500842 tig0027169:-11431 Msa1432730:CDS
ATACAGGAATTGAAAAGATA+AGG 0.505757 tig0027169:+13806 None:intergenic
TCGTATAAAGTTCAAAGAAG+TGG 0.508593 tig0027169:-10891 Msa1432730:CDS
TAGTTTAACTTCACGAGGAG+TGG 0.511679 tig0027169:+13633 None:intergenic
TATAGAAGTTCTTTGAAGCA+TGG 0.512144 tig0027169:+13334 None:intergenic
GAAGGATTAGGGAGGGTGCT+GGG 0.520089 tig0027169:-13459 Msa1432730:CDS
TTCTTTGAAGCATGGGAATG+GGG 0.526135 tig0027169:+13342 None:intergenic
ATGATAAACATGACAAAACT+AGG 0.530951 tig0027169:+10658 None:intergenic
CAAGGGCCTCATCGTAAGAA+TGG 0.533526 tig0027169:-11986 Msa1432730:CDS
CCGCGGTGGTGACAGCTGCC+GGG 0.534362 tig0027169:+11074 None:intergenic
ATAGAAGTTCTTTGAAGCAT+GGG 0.535113 tig0027169:+13335 None:intergenic
CCCTTCACCACACCGGTCAA+GGG 0.541833 tig0027169:-12003 Msa1432730:CDS
AAACCTCTTCATATCTTCAA+AGG 0.555864 tig0027169:+10736 None:intergenic
ATGGTGGATTGTACTGGTGA+GGG 0.557820 tig0027169:-13427 Msa1432730:CDS
GAAACTATTCATGAATGCCT+GGG 0.560588 tig0027169:-11497 Msa1432730:CDS
ATTACTATCCATTTGCAGGA+AGG 0.563934 tig0027169:-13477 Msa1432730:CDS
GGACAGAAGTCTAACTTGAA+AGG 0.564133 tig0027169:+13998 None:intergenic
ATCTTATGGTTATTAAGGAA+CGG 0.564453 tig0027169:-10958 Msa1432730:CDS
ATTAGGTTATGCTGTGGAGT+TGG 0.566011 tig0027169:-11032 Msa1432730:CDS
AGAAGAAAGATTGATGAACC+AGG 0.568559 tig0027169:+11320 None:intergenic
GGTTGCATGTAATGTGTGGT+GGG 0.570054 tig0027169:+11401 None:intergenic
GATGGTGGATTGTACTGGTG+AGG 0.570539 tig0027169:-13428 Msa1432730:CDS
TCACGAGGAGTGGAAGCAGC+TGG 0.571211 tig0027169:+13643 None:intergenic
AACTTGAAAGGATTTAGTAT+CGG 0.571421 tig0027169:+14010 None:intergenic
GGTGTTTATTTACAACTACA+AGG 0.572054 tig0027169:-10937 Msa1432730:CDS
TCCACAAAGCTTCCCCGCGG+TGG 0.581894 tig0027169:+11060 None:intergenic
CTTACGATGAGGCCCTTGAC+CGG 0.583352 tig0027169:+11991 None:intergenic
AAGTTGATGGTGGATTGTAC+TGG 0.588782 tig0027169:-13433 Msa1432730:CDS
TGTCCTTTGAAGATATGAAG+AGG 0.589462 tig0027169:-10739 Msa1432730:CDS
TGGTTGCATGTAATGTGTGG+TGG 0.590063 tig0027169:+11400 None:intergenic
AAAGAGTTAGATGAGATGCT+TGG 0.590438 tig0027169:-10710 Msa1432730:CDS
GGGATGATATAGGCTGCTCC+AGG 0.591783 tig0027169:+10809 None:intergenic
GGAAGAAGATACTACGACCC+TGG 0.596612 tig0027169:-11338 Msa1432730:CDS
GCATGAAACCACCGCACTTG+AGG 0.601841 tig0027169:+11563 None:intergenic
TTGAACCATACAATGAGCGA+TGG 0.603418 tig0027169:-11529 Msa1432730:CDS
GTACAATGACGAAGGTGAGA+TGG 0.604232 tig0027169:+11259 None:intergenic
TGCTGGGCGTAAGTTGATGG+TGG 0.605454 tig0027169:-13443 Msa1432730:CDS
CATACAATGAGCGATGGAGC+TGG 0.610758 tig0027169:-11523 Msa1432730:CDS
TCTTTGAAGCATGGGAATGG+GGG 0.613320 tig0027169:+13343 None:intergenic
AATATCCAACAAGTGGCGAA+TGG 0.616842 tig0027169:+11116 None:intergenic
GAAGAAAGATTGATGAACCA+GGG 0.619057 tig0027169:+11321 None:intergenic
ATCCATGTAAAATAAAGTTG+TGG 0.619116 tig0027169:-10442 Msa1432730:three_prime_UTR
TTACCGTAATATCCAACAAG+TGG 0.632459 tig0027169:+11109 None:intergenic
ATTAAAAGCTGAAGTTACAG+AGG 0.635766 tig0027169:-11002 Msa1432730:CDS
TGTAACACTTGATCAATTTG+GGG 0.637489 tig0027169:-13377 Msa1432730:CDS
TCTGACATAGATGATCAAGA+AGG 0.638298 tig0027169:-13607 Msa1432730:CDS
CGAGGAGTGGAAGCAGCTGG+TGG 0.639794 tig0027169:+13646 None:intergenic
TTGACCGGTGTGGTGAAGGG+AGG 0.647402 tig0027169:+12006 None:intergenic
GTTGAACCATTCTTACGATG+AGG 0.647528 tig0027169:+11980 None:intergenic
CATTATTGTTCACAGTGCGG+AGG 0.651919 tig0027169:-13687 Msa1432730:CDS
GCCTGGGCAGAAATGGCTCG+AGG 0.654434 tig0027169:-11481 Msa1432730:CDS
GCATCGTCAATGCACGCTCG+AGG 0.662333 tig0027169:-11150 Msa1432730:CDS
TCTACCTCCCTTCACCACAC+CGG 0.662546 tig0027169:-12010 Msa1432730:CDS
TTTGCAGGAAGGATTAGGGA+GGG 0.663911 tig0027169:-13466 Msa1432730:CDS
AAACTCATTAGGTTATGCTG+TGG 0.666130 tig0027169:-11038 Msa1432730:CDS
GATAATAGTTTAACTTCACG+AGG 0.669478 tig0027169:+13628 None:intergenic
TTACCTGTATGAGTCGAATG+GGG 0.671022 tig0027169:+13278 None:intergenic
CACCATTATTGTTCACAGTG+CGG 0.673411 tig0027169:-13690 Msa1432730:CDS
ATTTGCAGGAAGGATTAGGG+AGG 0.677174 tig0027169:-13467 Msa1432730:CDS
TGATGGTTGCATGTAATGTG+TGG 0.678806 tig0027169:+11397 None:intergenic
ATTACGTATTCATGAACACG+TGG 0.680316 tig0027169:+13971 None:intergenic
GTGACACGCCTCAAGTGCGG+TGG 0.681359 tig0027169:-11571 Msa1432730:CDS
CCTGTATGAGTCGAATGGGG+CGG 0.682735 tig0027169:+13281 None:intergenic
TAGGTGACACGCCTCAAGTG+CGG 0.682839 tig0027169:-11574 Msa1432730:CDS
TCGTAAGAATGGTTCAACGA+GGG 0.694699 tig0027169:-11975 Msa1432730:intron
CGAATGTCGTACAATGACGA+AGG 0.701737 tig0027169:+11251 None:intergenic
GATGAGGCCCTTGACCGGTG+TGG 0.732876 tig0027169:+11996 None:intergenic
GAATTGTGTACGTAAACCTG+TGG 0.747356 tig0027169:+13772 None:intergenic
CGGCAGCTGTCACCACCGCG+GGG 0.749713 tig0027169:-11072 Msa1432730:CDS
ATCGTAAGAATGGTTCAACG+AGG 0.760120 tig0027169:-11976 Msa1432730:CDS
GTTTCCACAAAGCTTCCCCG+CGG 0.764073 tig0027169:+11057 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATAAATTAAAAAA+TGG - tig0027169:12039-12058 Msa1432730:intron 0.0%
!!! ACATTTTAAGATATTATATT+TGG + tig0027169:11836-11855 None:intergenic 10.0%
!! ACTATATATAAAAAACATAA+GGG + tig0027169:12805-12824 None:intergenic 10.0%
!! TACTATATATAAAAAACATA+AGG + tig0027169:12806-12825 None:intergenic 10.0%
!!! AATCGCATTTTTTATTTTTT+GGG + tig0027169:10570-10589 None:intergenic 15.0%
!!! AAATCGCATTTTTTATTTTT+TGG + tig0027169:10571-10590 None:intergenic 15.0%
!!! TTTTTTTTATAATATGTTCC+AGG - tig0027169:11270-11289 Msa1432730:CDS 15.0%
!! TAAACCCTATTTAAAAAAAT+TGG - tig0027169:11808-11827 Msa1432730:intron 15.0%
!! ATGTATAAGTGATTTAAATT+TGG - tig0027169:11852-11871 Msa1432730:intron 15.0%
!! TGTATAAGTGATTTAAATTT+GGG - tig0027169:11853-11872 Msa1432730:intron 15.0%
!! AAAAGTTATGTAAATTAACA+TGG - tig0027169:11923-11942 Msa1432730:intron 15.0%
!! AAAGTTATGTAAATTAACAT+GGG - tig0027169:11924-11943 Msa1432730:intron 15.0%
!!! CTTTTTTTATTTTAGATTAG+AGG + tig0027169:12299-12318 None:intergenic 15.0%
!!! CTCTAATCTAAAATAAAAAA+AGG - tig0027169:12297-12316 Msa1432730:intron 15.0%
!! TAATACATGTAAATTATGAA+CGG - tig0027169:12574-12593 Msa1432730:intron 15.0%
!!! TATTATATTTGCTATATTGT+TGG + tig0027169:12701-12720 None:intergenic 15.0%
!! TATAACATATATCTTGATAT+TGG - tig0027169:12842-12861 Msa1432730:intron 15.0%
!!! TAAACTTTTACTTAAATTTG+AGG - tig0027169:13961-13980 Msa1432730:five_prime_UTR 15.0%
!!! ATTGGAATATACGATTTTTT+TGG + tig0027169:11589-11608 None:intergenic 20.0%
!! ATTCCATTTCAAAAAATTTG+TGG + tig0027169:11760-11779 None:intergenic 20.0%
!!! GGATCCAATTTTTTTAAATA+GGG + tig0027169:11815-11834 None:intergenic 20.0%
!!! TGGATCCAATTTTTTTAAAT+AGG + tig0027169:11816-11835 None:intergenic 20.0%
!! GTATAAGTGATTTAAATTTG+GGG - tig0027169:11854-11873 Msa1432730:intron 20.0%
!!! GTTTATGCAATTTTTTTTAG+AGG - tig0027169:12100-12119 Msa1432730:intron 20.0%
!!! ATTTTTTTTAGAGGATGTTT+AGG - tig0027169:12109-12128 Msa1432730:intron 20.0%
!!! TGATTTTCATGTAAAAAAGA+CGG - tig0027169:12514-12533 Msa1432730:intron 20.0%
!!! AAATTTGTTCTGTTTATAAC+TGG - tig0027169:12766-12785 Msa1432730:intron 20.0%
!!! TAAAAAACATAAGGGATTTT+GGG + tig0027169:12797-12816 None:intergenic 20.0%
!!! ATAAAAAACATAAGGGATTT+TGG + tig0027169:12798-12817 None:intergenic 20.0%
!! TATTGGATTCAAAATATGAT+AGG - tig0027169:12859-12878 Msa1432730:intron 20.0%
!! TCTATAAAGATTGATGTATT+TGG + tig0027169:13940-13959 None:intergenic 20.0%
!! GGATCACAAATAATTATTTA+CGG + tig0027169:13994-14013 None:intergenic 20.0%
! AACTTGAAAGGATTTAGTAT+CGG + tig0027169:10445-10464 None:intergenic 25.0%
! ATGAATATTATTGGGAAAAC+TGG + tig0027169:10545-10564 None:intergenic 25.0%
! TTTGGGTGATGAATATTATT+GGG + tig0027169:10553-10572 None:intergenic 25.0%
!!! TTTTGGGTGATGAATATTAT+TGG + tig0027169:10554-10573 None:intergenic 25.0%
!! ATAAAAAATGCGATTTTGCT+TGG - tig0027169:10575-10594 Msa1432730:three_prime_UTR 25.0%
! ATACAGGAATTGAAAAGATA+AGG + tig0027169:10649-10668 None:intergenic 25.0%
!!! TTTCAATTCCTGTATTTTTG+TGG - tig0027169:10654-10673 Msa1432730:three_prime_UTR 25.0%
! CATGTCTAAGAACTTTAATA+GGG + tig0027169:10934-10953 None:intergenic 25.0%
! ATGTAACACTTGATCAATTT+GGG - tig0027169:11074-11093 Msa1432730:CDS 25.0%
! AGTGAGTATGTCAAATTTAT+TGG + tig0027169:11249-11268 None:intergenic 25.0%
! CATCTGTAACAACATAAAAT+TGG + tig0027169:11607-11626 None:intergenic 25.0%
!! CAAAATCACATCAATTTTCT+AGG - tig0027169:12178-12197 Msa1432730:intron 25.0%
! TATTAGCGCAATAAAAAGAA+CGG + tig0027169:12559-12578 None:intergenic 25.0%
! AAATATGTTCGTATTCATGA+TGG + tig0027169:13075-13094 None:intergenic 25.0%
!! ATCTTATCACTTCATGTTTT+TGG - tig0027169:13224-13243 Msa1432730:intron 25.0%
! ATCTTATGGTTATTAAGGAA+CGG - tig0027169:13494-13513 Msa1432730:CDS 25.0%
! CAAAATTAACCTAAAGCAAA+TGG - tig0027169:13769-13788 Msa1432730:five_prime_UTR 25.0%
! ATGATAAACATGACAAAACT+AGG + tig0027169:13797-13816 None:intergenic 25.0%
! ATCCATGTAAAATAAAGTTG+TGG - tig0027169:14010-14029 Msa1432730:five_prime_UTR 25.0%
ACTTGACAAGATTTAGTTAC+AGG + tig0027169:10416-10435 None:intergenic 30.0%
AAACTGGCACATGAAATTTA+AGG + tig0027169:10529-10548 None:intergenic 30.0%
GTTTATATCACATGCTTACA+TGG - tig0027169:10622-10641 Msa1432730:three_prime_UTR 30.0%
! GATAATAGTTTAACTTCACG+AGG + tig0027169:10827-10846 None:intergenic 30.0%
TGACGATAGATAAACATCAT+AGG + tig0027169:10887-10906 None:intergenic 30.0%
!!! GGGTTTTTTTCTTTCATTGA+CGG + tig0027169:10914-10933 None:intergenic 30.0%
GCATGTCTAAGAACTTTAAT+AGG + tig0027169:10935-10954 None:intergenic 30.0%
GATGTAACACTTGATCAATT+TGG - tig0027169:11073-11092 Msa1432730:CDS 30.0%
TGTAACACTTGATCAATTTG+GGG - tig0027169:11075-11094 Msa1432730:CDS 30.0%
!! ATAGAAGTTCTTTGAAGCAT+GGG + tig0027169:11120-11139 None:intergenic 30.0%
!! TATAGAAGTTCTTTGAAGCA+TGG + tig0027169:11121-11140 None:intergenic 30.0%
!! TCAATAATTAGTTCTGATCC+TGG + tig0027169:11151-11170 None:intergenic 30.0%
TATGTAATTAGTTCAGATCC+TGG + tig0027169:11291-11310 None:intergenic 30.0%
TTTATGTTGTTACAGATGCA+TGG - tig0027169:11609-11628 Msa1432730:intron 30.0%
! GTCATCTTTTGTTAAAGGTT+TGG + tig0027169:11645-11664 None:intergenic 30.0%
! TAGAGGTCATCTTTTGTTAA+AGG + tig0027169:11650-11669 None:intergenic 30.0%
ATCTTTAAGCAACTCTTTAG+AGG + tig0027169:11667-11686 None:intergenic 30.0%
!!! GCTCCACAAATTTTTTGAAA+TGG - tig0027169:11754-11773 Msa1432730:intron 30.0%
GTCATCGTAAAAGAAAAACA+TGG + tig0027169:12677-12696 None:intergenic 30.0%
ATTAAAAGCTGAAGTTACAG+AGG - tig0027169:13450-13469 Msa1432730:CDS 30.0%
AGCAGATCTTATGGTTATTA+AGG - tig0027169:13489-13508 Msa1432730:CDS 30.0%
GGTGTTTATTTACAACTACA+AGG - tig0027169:13515-13534 Msa1432730:CDS 30.0%
TCGTATAAAGTTCAAAGAAG+TGG - tig0027169:13561-13580 Msa1432730:CDS 30.0%
!! AAGTTCAAAGAAGTGGATTT+TGG - tig0027169:13568-13587 Msa1432730:CDS 30.0%
ATTCTTATGTGGGATGATAT+AGG + tig0027169:13656-13675 None:intergenic 30.0%
ATCCCACATAAGAATGTAAA+AGG - tig0027169:13661-13680 Msa1432730:CDS 30.0%
! CTCCTTTTACATTCTTATGT+GGG + tig0027169:13666-13685 None:intergenic 30.0%
! TCTCCTTTTACATTCTTATG+TGG + tig0027169:13667-13686 None:intergenic 30.0%
AAGAATGTAAAAGGAGAAGA+AGG - tig0027169:13670-13689 Msa1432730:CDS 30.0%
AAACCTCTTCATATCTTCAA+AGG + tig0027169:13719-13738 None:intergenic 30.0%
!! CTTTTACTTAAATTTGAGGC+TGG - tig0027169:13965-13984 Msa1432730:five_prime_UTR 30.0%
!!! GTCCACAACTTTATTTTACA+TGG + tig0027169:14015-14034 None:intergenic 30.0%
TATTCATGAACACGTGGAAT+AGG + tig0027169:10478-10497 None:intergenic 35.0%
ATTACGTATTCATGAACACG+TGG + tig0027169:10484-10503 None:intergenic 35.0%
TCTGACATAGATGATCAAGA+AGG - tig0027169:10845-10864 Msa1432730:CDS 35.0%
GTGTATTACTATCCATTTGC+AGG - tig0027169:10971-10990 Msa1432730:CDS 35.0%
ATTACTATCCATTTGCAGGA+AGG - tig0027169:10975-10994 Msa1432730:CDS 35.0%
TTTACCTGTATGAGTCGAAT+GGG + tig0027169:11178-11197 None:intergenic 35.0%
! TTTTACCTGTATGAGTCGAA+TGG + tig0027169:11179-11198 None:intergenic 35.0%
GAATGCGATAATTGAAACGT+AGG + tig0027169:11227-11246 None:intergenic 35.0%
ACATGAGAAATCAACATGCA+TGG - tig0027169:12241-12260 Msa1432730:intron 35.0%
TGAAACTATTCATGAATGCC+TGG - tig0027169:12954-12973 Msa1432730:intron 35.0%
GAAACTATTCATGAATGCCT+GGG - tig0027169:12955-12974 Msa1432730:intron 35.0%
CATTCAACCTGTATGGAATA+GGG - tig0027169:13009-13028 Msa1432730:intron 35.0%
ATGGAATAGGGAGATTCTAA+TGG - tig0027169:13021-13040 Msa1432730:intron 35.0%
! TTTTTCGTCAAATACCGTCA+AGG - tig0027169:13093-13112 Msa1432730:intron 35.0%
GAAGAAAGATTGATGAACCA+GGG + tig0027169:13134-13153 None:intergenic 35.0%
AGAAGAAAGATTGATGAACC+AGG + tig0027169:13135-13154 None:intergenic 35.0%
TTACCGTAATATCCAACAAG+TGG + tig0027169:13346-13365 None:intergenic 35.0%
! AAGCTTTGTGGAAACTCATT+AGG - tig0027169:13403-13422 Msa1432730:CDS 35.0%
AAACTCATTAGGTTATGCTG+TGG - tig0027169:13414-13433 Msa1432730:CDS 35.0%
!! TCAAAGAAGTGGATTTTGGA+TGG - tig0027169:13572-13591 Msa1432730:CDS 35.0%
!! CAAAGAAGTGGATTTTGGAT+GGG - tig0027169:13573-13592 Msa1432730:CDS 35.0%
!! AAAGAAGTGGATTTTGGATG+GGG - tig0027169:13574-13593 Msa1432730:CDS 35.0%
! TGTCCTTTGAAGATATGAAG+AGG - tig0027169:13713-13732 Msa1432730:exon 35.0%
AAAGAGTTAGATGAGATGCT+TGG - tig0027169:13742-13761 Msa1432730:five_prime_UTR 35.0%
AACTAGGAACCATTTGCTTT+AGG + tig0027169:13781-13800 None:intergenic 35.0%
GGACAGAAGTCTAACTTGAA+AGG + tig0027169:10457-10476 None:intergenic 40.0%
TGTGGTGACCACAAAAATAC+AGG + tig0027169:10665-10684 None:intergenic 40.0%
!! TGTATTTTTGTGGTCACCAC+AGG - tig0027169:10664-10683 Msa1432730:three_prime_UTR 40.0%
GAATTGTGTACGTAAACCTG+TGG + tig0027169:10683-10702 None:intergenic 40.0%
CACCATTATTGTTCACAGTG+CGG - tig0027169:10762-10781 Msa1432730:CDS 40.0%
! TAGTTTAACTTCACGAGGAG+TGG + tig0027169:10822-10841 None:intergenic 40.0%
ATCCATTTGCAGGAAGGATT+AGG - tig0027169:10981-11000 Msa1432730:CDS 40.0%
TCCATTTGCAGGAAGGATTA+GGG - tig0027169:10982-11001 Msa1432730:CDS 40.0%
!! AAGTTGATGGTGGATTGTAC+TGG - tig0027169:11019-11038 Msa1432730:CDS 40.0%
!! TTCTTTGAAGCATGGGAATG+GGG + tig0027169:11113-11132 None:intergenic 40.0%
!! GTTCTTTGAAGCATGGGAAT+GGG + tig0027169:11114-11133 None:intergenic 40.0%
!! AGTTCTTTGAAGCATGGGAA+TGG + tig0027169:11115-11134 None:intergenic 40.0%
GAACTTCTATACGATGTTCC+AGG - tig0027169:11130-11149 Msa1432730:CDS 40.0%
TTACCTGTATGAGTCGAATG+GGG + tig0027169:11177-11196 None:intergenic 40.0%
! ATGAGTGTACGCCTTTTAAG+TGG - tig0027169:11709-11728 Msa1432730:intron 40.0%
GTTGAACCATTCTTACGATG+AGG + tig0027169:12475-12494 None:intergenic 40.0%
ATCGTAAGAATGGTTCAACG+AGG - tig0027169:12476-12495 Msa1432730:intron 40.0%
TCGTAAGAATGGTTCAACGA+GGG - tig0027169:12477-12496 Msa1432730:intron 40.0%
!! TCAACGAGGGTATTCAGATA+AGG - tig0027169:12490-12509 Msa1432730:intron 40.0%
TTGAACCATACAATGAGCGA+TGG - tig0027169:12923-12942 Msa1432730:intron 40.0%
AACCTTCCATTCAACCTGTA+TGG - tig0027169:13002-13021 Msa1432730:intron 40.0%
! TTCCATACAGGTTGAATGGA+AGG + tig0027169:13007-13026 None:intergenic 40.0%
! CCTATTCCATACAGGTTGAA+TGG + tig0027169:13011-13030 None:intergenic 40.0%
CCATTCAACCTGTATGGAAT+AGG - tig0027169:13008-13027 Msa1432730:intron 40.0%
AGAATCTCCCTATTCCATAC+AGG + tig0027169:13019-13038 None:intergenic 40.0%
TGATGGTTGCATGTAATGTG+TGG + tig0027169:13058-13077 None:intergenic 40.0%
CGTAGTATCTTCTTCCTTGA+CGG + tig0027169:13110-13129 None:intergenic 40.0%
! CTAACTGCAATGCTTTTGTG+CGG + tig0027169:13255-13274 None:intergenic 40.0%
!! CACAAAAGCATTGCAGTTAG+AGG - tig0027169:13255-13274 Msa1432730:intron 40.0%
AATATCCAACAAGTGGCGAA+TGG + tig0027169:13339-13358 None:intergenic 40.0%
! TCGCCACTTGTTGGATATTA+CGG - tig0027169:13340-13359 Msa1432730:CDS 40.0%
!! CGGTAATTGTTTTGCTTACC+CGG - tig0027169:13360-13379 Msa1432730:CDS 40.0%
ATTAGGTTATGCTGTGGAGT+TGG - tig0027169:13420-13439 Msa1432730:CDS 40.0%
GCATTCTGTAGCAGATCTTA+TGG - tig0027169:13480-13499 Msa1432730:CDS 40.0%
TGCTTGGCAACGACACAATA+AGG - tig0027169:10591-10610 Msa1432730:three_prime_UTR 45.0%
CTCCGCACTGTGAACAATAA+TGG + tig0027169:10767-10786 None:intergenic 45.0%
CATTATTGTTCACAGTGCGG+AGG - tig0027169:10765-10784 Msa1432730:CDS 45.0%
TCCCTAATCCTTCCTGCAAA+TGG + tig0027169:10986-11005 None:intergenic 45.0%
ATTTGCAGGAAGGATTAGGG+AGG - tig0027169:10985-11004 Msa1432730:CDS 45.0%
TTTGCAGGAAGGATTAGGGA+GGG - tig0027169:10986-11005 Msa1432730:CDS 45.0%
!! ATGGTGGATTGTACTGGTGA+GGG - tig0027169:11025-11044 Msa1432730:CDS 45.0%
!! TCTTTGAAGCATGGGAATGG+GGG + tig0027169:11112-11131 None:intergenic 45.0%
!! AGTGTACGCCTTTTAAGTGG+CGG - tig0027169:11712-11731 Msa1432730:intron 45.0%
GGTTGCATGTAATGTGTGGT+GGG + tig0027169:13054-13073 None:intergenic 45.0%
TGGTTGCATGTAATGTGTGG+TGG + tig0027169:13055-13074 None:intergenic 45.0%
GTACAATGACGAAGGTGAGA+TGG + tig0027169:13196-13215 None:intergenic 45.0%
CGAATGTCGTACAATGACGA+AGG + tig0027169:13204-13223 None:intergenic 45.0%
! TATGGTGGAGCTGCAAAAAG+TGG - tig0027169:13607-13626 Msa1432730:CDS 45.0%
! ATGGTGGAGCTGCAAAAAGT+GGG - tig0027169:13608-13627 Msa1432730:CDS 45.0%
ATATAGGCTGCTCCAGGAAA+AGG + tig0027169:13640-13659 None:intergenic 45.0%
!!! TTTTATATTTATTATATTGT+TGG + tig0027169:11476-11495 None:intergenic 5.0%
!! GATGGTGGATTGTACTGGTG+AGG - tig0027169:11024-11043 Msa1432730:CDS 50.0%
CAAGGGCCTCATCGTAAGAA+TGG - tig0027169:12466-12485 Msa1432730:intron 50.0%
CAGCTCCATCGCTCATTGTA+TGG + tig0027169:12931-12950 None:intergenic 50.0%
CATACAATGAGCGATGGAGC+TGG - tig0027169:12929-12948 Msa1432730:intron 50.0%
CATGAATGCCTGGGCAGAAA+TGG - tig0027169:12964-12983 Msa1432730:intron 50.0%
GGAAGAAGATACTACGACCC+TGG - tig0027169:13114-13133 Msa1432730:intron 50.0%
GTCAACCATTCGCCACTTGT+TGG - tig0027169:13331-13350 Msa1432730:CDS 50.0%
!! AGTGGATTTTGGATGGGGTG+AGG - tig0027169:13579-13598 Msa1432730:CDS 50.0%
TGGTGGAGCTGCAAAAAGTG+GGG - tig0027169:13609-13628 Msa1432730:CDS 50.0%
!! GAAGGATTAGGGAGGGTGCT+GGG - tig0027169:10993-11012 Msa1432730:CDS 55.0%
!! TGCTGGGCGTAAGTTGATGG+TGG - tig0027169:11009-11028 Msa1432730:CDS 55.0%
CCTGTATGAGTCGAATGGGG+CGG + tig0027169:11174-11193 None:intergenic 55.0%
GTGTGGCTCCGCCACTTAAA+AGG + tig0027169:11723-11742 None:intergenic 55.0%
CGGAGCCACACACAACATGA+GGG - tig0027169:11732-11751 Msa1432730:intron 55.0%
TGGAGCCCTCATGTTGTGTG+TGG + tig0027169:11740-11759 None:intergenic 55.0%
TCTACCTCCCTTCACCACAC+CGG - tig0027169:12442-12461 Msa1432730:intron 55.0%
CTTACGATGAGGCCCTTGAC+CGG + tig0027169:12464-12483 None:intergenic 55.0%
TAGGTGACACGCCTCAAGTG+CGG - tig0027169:12878-12897 Msa1432730:intron 55.0%
GCATGAAACCACCGCACTTG+AGG + tig0027169:12892-12911 None:intergenic 55.0%
CATAGCTGCGCTTCGTCTCT+TGG - tig0027169:13168-13187 Msa1432730:intron 55.0%
GTTTCCACAAAGCTTCCCCG+CGG + tig0027169:13398-13417 None:intergenic 55.0%
! GGATTTTGGATGGGGTGAGG+CGG - tig0027169:13582-13601 Msa1432730:CDS 55.0%
GAGCTGCAAAAAGTGGGGCT+GGG - tig0027169:13614-13633 Msa1432730:CDS 55.0%
GGGATGATATAGGCTGCTCC+AGG + tig0027169:13646-13665 None:intergenic 55.0%
TCACGAGGAGTGGAAGCAGC+TGG + tig0027169:10812-10831 None:intergenic 60.0%
! GGAAGGATTAGGGAGGGTGC+TGG - tig0027169:10992-11011 Msa1432730:CDS 60.0%
!! GGGTGCTGGGCGTAAGTTGA+TGG - tig0027169:11006-11025 Msa1432730:CDS 60.0%
CCGCCCCATTCGACTCATAC+AGG - tig0027169:11171-11190 Msa1432730:CDS 60.0%
GCGGAGCCACACACAACATG+AGG - tig0027169:11731-11750 Msa1432730:intron 60.0%
TTGACCGGTGTGGTGAAGGG+AGG + tig0027169:12449-12468 None:intergenic 60.0%
TCCCTTCACCACACCGGTCA+AGG - tig0027169:12448-12467 Msa1432730:intron 60.0%
CCCTTGACCGGTGTGGTGAA+GGG + tig0027169:12452-12471 None:intergenic 60.0%
CCCTTCACCACACCGGTCAA+GGG - tig0027169:12449-12468 Msa1432730:intron 60.0%
TCCTCGAGCCATTTCTGCCC+AGG + tig0027169:12975-12994 None:intergenic 60.0%
GCATCGTCAATGCACGCTCG+AGG - tig0027169:13302-13321 Msa1432730:CDS 60.0%
GGAGCTGCAAAAAGTGGGGC+TGG - tig0027169:13613-13632 Msa1432730:CDS 60.0%
! CTGGTGGCACCAGCTCTGGT+TGG + tig0027169:10793-10812 None:intergenic 65.0%
CGAGGAGTGGAAGCAGCTGG+TGG + tig0027169:10809-10828 None:intergenic 65.0%
GCCCTTGACCGGTGTGGTGA+AGG + tig0027169:12453-12472 None:intergenic 65.0%
GATGAGGCCCTTGACCGGTG+TGG + tig0027169:12459-12478 None:intergenic 65.0%
GTGACACGCCTCAAGTGCGG+TGG - tig0027169:12881-12900 Msa1432730:intron 65.0%
GCCTGGGCAGAAATGGCTCG+AGG - tig0027169:12971-12990 Msa1432730:intron 65.0%
ACCACCGCGGGGAAGCTTTG+TGG - tig0027169:13391-13410 Msa1432730:CDS 65.0%
TCCACAAAGCTTCCCCGCGG+TGG + tig0027169:13395-13414 None:intergenic 65.0%
GGATGGGGTGAGGCGGTGTA+TGG - tig0027169:13589-13608 Msa1432730:CDS 65.0%
TGGGGTGAGGCGGTGTATGG+TGG - tig0027169:13592-13611 Msa1432730:CDS 65.0%
! AGTGGGGCTGGGCCTTTTCC+TGG - tig0027169:13625-13644 Msa1432730:CDS 65.0%
GCGGAGGAGCCAACCAGAGC+TGG - tig0027169:10781-10800 Msa1432730:CDS 70.0%
GCAGCTGGTGGCACCAGCTC+TGG + tig0027169:10797-10816 None:intergenic 70.0%
CCGCGGTGGTGACAGCTGCC+GGG + tig0027169:13381-13400 None:intergenic 75.0%
CCCGGCAGCTGTCACCACCG+CGG - tig0027169:13378-13397 Msa1432730:CDS 75.0%
CCCGCGGTGGTGACAGCTGC+CGG + tig0027169:13382-13401 None:intergenic 75.0%
CCGGCAGCTGTCACCACCGC+GGG - tig0027169:13379-13398 Msa1432730:CDS 75.0%
CGGCAGCTGTCACCACCGCG+GGG - tig0027169:13380-13399 Msa1432730:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0027169 gene 10407 14067 10407 ID=Msa1432730;Name=Msa1432730
tig0027169 mRNA 10407 14067 10407 ID=Msa1432730-mRNA-1;Parent=Msa1432730;Name=Msa1432730-mRNA-1;_AED=0.04;_eAED=0.04;_QI=345|0|0.66|0.66|1|1|3|290|464
tig0027169 exon 10407 11593 10407 ID=Msa1432730-mRNA-1:exon:8254;Parent=Msa1432730-mRNA-1
tig0027169 exon 11976 12032 11976 ID=Msa1432730-mRNA-1:exon:8253;Parent=Msa1432730-mRNA-1
tig0027169 exon 13282 14067 13282 ID=Msa1432730-mRNA-1:exon:8252;Parent=Msa1432730-mRNA-1
tig0027169 five_prime_UTR 13723 14067 13723 ID=Msa1432730-mRNA-1:five_prime_utr;Parent=Msa1432730-mRNA-1
tig0027169 CDS 13282 13722 13282 ID=Msa1432730-mRNA-1:cds;Parent=Msa1432730-mRNA-1
tig0027169 CDS 11976 12032 11976 ID=Msa1432730-mRNA-1:cds;Parent=Msa1432730-mRNA-1
tig0027169 CDS 10697 11593 10697 ID=Msa1432730-mRNA-1:cds;Parent=Msa1432730-mRNA-1
tig0027169 three_prime_UTR 10407 10696 10407 ID=Msa1432730-mRNA-1:three_prime_utr;Parent=Msa1432730-mRNA-1
Gene Sequence

>Msa1432730

ATGACTTCAGCACCATTATTGTTCACAGTGCGGAGGAGCCAACCAGAGCTGGTGCCACCAGCTGCTTCCACTCCTCGTGAAGTTAAACTATTATCTGACATAGATGATCAAGAAGGCTTGCGTTTCAATATTCCTATGATGTTTATCTATCGTCATGAACCGTCAATGAAAGAAAAAAACCCTATTAAAGTTCTTAGACATGCACTCTCACAAGCACTTGTGTATTACTATCCATTTGCAGGAAGGATTAGGGAGGGTGCTGGGCGTAAGTTGATGGTGGATTGTACTGGTGAGGGTGTCATGTTCATTGAAGCTGAAGCTGATGTAACACTTGATCAATTTGGGGACGCTTTGCATCCCCCATTCCCATGCTTCAAAGAACTTCTATACGATGTTCCAGGATCAGAACTAATTATTGACCGCCCCATTCGACTCATACAGTCTACCTCCCTTCACCACACCGGTCAAGGGCCTCATCGTAAGAATGGTTCAACGAGGGTGACACGCCTCAAGTGCGGTGGTTTCATGCTAGTCGTGTATTTGAACCATACAATGAGCGATGGAGCTGGTTTGAAACTATTCATGAATGCCTGGGCAGAAATGGCTCGAGGAGCACATAAACCTTCCATTCAACCTGTATGGAATAGGGAGATTCTAATGGCAAGAGACCCACCACACATTACATGCAACCATCATGAATACGAACATATTTTTTCGTCAAATACCGTCAAGGAAGAAGATACTACGACCCTGGTTCATCAATCTTTCTTCTTTAGAACTTCTGACATAGCTGCGCTTCGTCTCTTGGTTCCATCTCACCTTCGTCATTGTACGACATTCGATCTTATCACTTCATGTTTTTGGTGCTGCCGCACAAAAGCATTGCAGTTAGAGGCAGATGAAGAGATACGAATGATGTGCATCGTCAATGCACGCTCGAGGTTTAGCGTCAACCATTCGCCACTTGTTGGATATTACGGTAATTGTTTTGCTTACCCGGCAGCTGTCACCACCGCGGGGAAGCTTTGTGGAAACTCATTAGGTTATGCTGTGGAGTTGGTGAGAAAATTAAAAGCTGAAGTTACAGAGGAATACATGCATTCTGTAGCAGATCTTATGGTTATTAAGGAACGGTGTTTATTTACAACTACAAGGTCTTGTGTTGTTTCAGATGTGACTCGTATAAAGTTCAAAGAAGTGGATTTTGGATGGGGTGAGGCGGTGTATGGTGGAGCTGCAAAAAGTGGGGCTGGGCCTTTTCCTGGAGCAGCCTATATCATCCCACATAAGAATGTAAAAGGAGAAGAAGGTTTCATATTACTAGTTTGTTTGTCCTTTGAAGATATGAAGAGGTTTGCTAAAGAGTTAGATGAGATGCTTGGTAAACAAAATTAA
Protein sequence

>Msa1432730

MTSAPLLFTVRRSQPELVPPAASTPREVKLLSDIDDQEGLRFNIPMMFIYRHEPSMKEKNPIKVLRHALSQALVYYYPFAGRIREGAGRKLMVDCTGEGVMFIEAEADVTLDQFGDALHPPFPCFKELLYDVPGSELIIDRPIRLIQSTSLHHTGQGPHRKNGSTRVTRLKCGGFMLVVYLNHTMSDGAGLKLFMNAWAEMARGAHKPSIQPVWNREILMARDPPHITCNHHEYEHIFSSNTVKEEDTTTLVHQSFFFRTSDIAALRLLVPSHLRHCTTFDLITSCFWCCRTKALQLEADEEIRMMCIVNARSRFSVNHSPLVGYYGNCFAYPAAVTTAGKLCGNSLGYAVELVRKLKAEVTEEYMHSVADLMVIKERCLFTTTRSCVVSDVTRIKFKEVDFGWGEAVYGGAAKSGAGPFPGAAYIIPHKNVKGEEGFILLVCLSFEDMKRFAKELDEMLGKQN*