AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1171640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171640 MtrunA17_Chr3g0112121 97.941 437 8 1 1 437 1 436 0.0 885
Msa1171640 MtrunA17_Chr5g0435631 89.318 440 40 4 1 437 1 436 0.0 813
Msa1171640 MtrunA17_Chr4g0009431 83.105 438 72 2 1 437 1 437 0.0 756
Msa1171640 MtrunA17_Chr2g0334101 45.721 409 198 13 25 420 17 414 7.30e-111 332
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171640 AT2G39730.2 80.137 438 83 2 1 437 1 435 0.0 730
Msa1171640 AT2G39730.3 80.137 438 83 2 1 437 1 435 0.0 729
Msa1171640 AT2G39730.1 80.137 438 83 2 1 437 1 435 0.0 729
Msa1171640 AT1G73110.2 54.636 302 130 6 125 420 31 331 3.50e-114 338
Msa1171640 AT1G73110.1 54.636 302 130 6 125 420 128 428 1.13e-112 338

Find 134 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATGTTCATCATATAAT+TGG 0.129837 8_1:-1468172 None:intergenic
GTACAATGTTTAATTAAATT+TGG 0.183260 8_1:+1468065 Msa1171640:three_prime_UTR
GAAGTGAGGAAGTGGGTTTC+TGG 0.222183 8_1:+1467526 Msa1171640:CDS
CCAATTGGCAGACAAGTATT+TGG 0.242423 8_1:+1467690 Msa1171640:CDS
TGATAGGTTCCTCTCATTCT+TGG 0.246953 8_1:+1466203 Msa1171640:intron
TTCCTCTCATTCTTGGAGTC+TGG 0.255291 8_1:+1466210 Msa1171640:CDS
AACATTCTCAATGTGTTATT+AGG 0.258499 8_1:+1467961 Msa1171640:three_prime_UTR
TTCCTCTTGTGATGTCTTGT+TGG 0.298884 8_1:-1465800 None:intergenic
TCCTCTCATTCTTGGAGTCT+GGG 0.312740 8_1:+1466211 Msa1171640:CDS
TCATCATATAATTGGGATAA+TGG 0.322524 8_1:-1468164 None:intergenic
TCTAGTTTCTCCAATGATCT+TGG 0.338522 8_1:+1467764 Msa1171640:exon
TGCTAATCAAGATGCTATTA+AGG 0.353818 8_1:+1467732 Msa1171640:CDS
AACTCACTTGATTCCCATCT+TGG 0.355485 8_1:-1466279 None:intergenic
GTGAAGCCTCTGACATTATC+AGG 0.368067 8_1:+1467006 Msa1171640:CDS
GGAGTTGGTATTGAAACAAT+TGG 0.369327 8_1:+1467547 Msa1171640:CDS
GCTGCTTCAGTTTCAACTGT+TGG 0.393971 8_1:+1464702 Msa1171640:CDS
ATCTTTGGCTGCTCAAACAC+TGG 0.394520 8_1:-1467601 None:intergenic
AAGATTGTTGACACCTTCCC+TGG 0.404593 8_1:+1467368 Msa1171640:CDS
CTAGAAAAGCTATTGGAGTA+TGG 0.406206 8_1:+1467628 Msa1171640:CDS
TGAGTGTGGCATTCACCATT+TGG 0.411461 8_1:-1467115 None:intergenic
CGGAGACAGATGGAGGGGTT+TGG 0.414870 8_1:+1465758 Msa1171640:CDS
GTACAACTTTGACAACACTA+TGG 0.415644 8_1:+1466008 Msa1171640:CDS
GATGACACTAGAAAAGCTAT+TGG 0.417148 8_1:+1467621 Msa1171640:CDS
CACATGTTCATCATATAATT+GGG 0.419749 8_1:-1468171 None:intergenic
TTGAATGGCTCAGTAGTATC+TGG 0.424879 8_1:+1465600 Msa1171640:CDS
CCAATACGATCTTCTCTAGT+AGG 0.432006 8_1:-1467293 None:intergenic
AGGAAGTGGGTTTCTGGAGT+TGG 0.432162 8_1:+1467532 Msa1171640:CDS
GCTATTATTGCAGATTTCTT+CGG 0.434056 8_1:+1467475 Msa1171640:intron
CCAAATACTTGTCTGCCAAT+TGG 0.435805 8_1:-1467690 None:intergenic
GTTTCTAATTCAGCTTTCTT+CGG 0.438269 8_1:+1465630 Msa1171640:CDS
GAATGTGAAGAGAGTCCAAT+TGG 0.441295 8_1:+1467675 Msa1171640:CDS
CTCTCCAGCACTCATCATGA+TGG 0.446603 8_1:-1466931 None:intergenic
TAATAAAATTATCTCAAACT+TGG 0.449697 8_1:-1467999 None:intergenic
TTCCAGCATCTTGTGGTGCT+TGG 0.450580 8_1:-1465845 None:intergenic
AATTTACATACCTTGATGTT+GGG 0.454354 8_1:-1466102 None:intergenic
GGGTAGGGTCAAGAAATTCT+TGG 0.455278 8_1:-1466082 None:intergenic
AAGTGTTGAGAAATCATTAC+CGG 0.455328 8_1:-1467222 None:intergenic
TTACAAACCTCCAAGATCAT+TGG 0.458612 8_1:-1467774 None:intergenic
GTCGTATGGAGAAGTTCTAC+TGG 0.461112 8_1:+1467267 Msa1171640:CDS
AAATTTACATACCTTGATGT+TGG 0.464290 8_1:-1466103 None:intergenic
AACTTTGACAACACTATGGA+TGG 0.466779 8_1:+1466012 Msa1171640:CDS
CCAGGGAGTTGGACATTGGT+GGG 0.468336 8_1:-1467155 None:intergenic
ATCATTACCGGTGACAATGA+TGG 0.469176 8_1:-1467210 None:intergenic
GCCATGGATACTGCACTGAT+TGG 0.470784 8_1:-1464681 None:intergenic
TGAGCTTGTCTTTGCCAAGA+TGG 0.475008 8_1:+1466265 Msa1171640:CDS
AGTAGTTGCATCGAAGTTTG+AGG 0.476019 8_1:-1467913 None:intergenic
TCTTCCTTGTTGTACATACC+AGG 0.482898 8_1:-1467173 None:intergenic
TCTTATGAGTACATTAGTAC+TGG 0.483378 8_1:+1465888 Msa1171640:CDS
TTCCAAGCACCACAAGATGC+TGG 0.484718 8_1:+1465843 Msa1171640:CDS
CAGATTTCTTCGGTGCATTG+AGG 0.486126 8_1:+1467485 Msa1171640:intron
CATCACAAGAGGAAAGGGTT+TGG 0.488621 8_1:+1465809 Msa1171640:CDS
AGTTTCTCCAATGATCTTGG+AGG 0.492672 8_1:+1467767 Msa1171640:exon
CGATGCAGGAGCAGGTCGTA+TGG 0.496017 8_1:+1467064 Msa1171640:CDS
TTCATCAACGATCTCGATGC+AGG 0.496807 8_1:+1467050 Msa1171640:CDS
TGATAATGTCAGAGGCTTCA+CGG 0.497210 8_1:-1467004 None:intergenic
AGATTTCTTCGGTGCATTGA+GGG 0.497710 8_1:+1467486 Msa1171640:intron
AACGATCTCGATGCAGGAGC+AGG 0.497933 8_1:+1467056 Msa1171640:CDS
TTGGTCATCTGAAATATCAT+AGG 0.498519 8_1:-1465781 None:intergenic
GATCGTATTGGTGTCTGCAT+TGG 0.499271 8_1:+1467305 Msa1171640:CDS
AGCCCCATCATGATGAGTGC+TGG 0.499726 8_1:+1466927 Msa1171640:intron
TCCTTTCCTGATAATGTCAG+AGG 0.502457 8_1:-1467012 None:intergenic
GAGCTTGAAAGTGGAAATGC+AGG 0.508166 8_1:+1466951 Msa1171640:CDS
GAGCTTGTCTTTGCCAAGAT+GGG 0.509304 8_1:+1466266 Msa1171640:CDS
TCTAATAGGTAGAAAGAGAA+AGG 0.515383 8_1:-1468090 None:intergenic
TCATTACCGGTGACAATGAT+GGG 0.516193 8_1:-1467209 None:intergenic
TCTCATCCGTGATGGTCGTA+TGG 0.520006 8_1:+1467253 Msa1171640:CDS
TCTCCAGCACTCATCATGAT+GGG 0.521950 8_1:-1466930 None:intergenic
AGATAGGTATACCGATGGAT+TGG 0.522987 8_1:-1467391 None:intergenic
CAAGACATCACAAGAGGAAA+GGG 0.523453 8_1:+1465804 Msa1171640:CDS
ACATACCTTGATGTTGGGTA+GGG 0.523802 8_1:-1466097 None:intergenic
CCAAACACAAAAGTTTCCTC+AGG 0.524555 8_1:+1465684 Msa1171640:CDS
TCCAATCAGTGCAGTATCCA+TGG 0.525087 8_1:+1464680 Msa1171640:exon
TGGCAAAGACAAGCTCAGCT+TGG 0.525244 8_1:-1466259 None:intergenic
TACATACCTTGATGTTGGGT+AGG 0.527306 8_1:-1466098 None:intergenic
GCCTCTGACATTATCAGGAA+AGG 0.527871 8_1:+1467011 Msa1171640:CDS
TATTTGGAAGGTGCTGCTCT+TGG 0.530394 8_1:+1467706 Msa1171640:CDS
ATACACTGTCAACAACCAAA+TGG 0.531515 8_1:+1467100 Msa1171640:CDS
ATTCTCAATGTGTTATTAGG+TGG 0.537623 8_1:+1467964 Msa1171640:three_prime_UTR
CCTCTCATTCTTGGAGTCTG+GGG 0.539615 8_1:+1466212 Msa1171640:CDS
ACCAGGGAGTTGGACATTGG+TGG 0.539763 8_1:-1467156 None:intergenic
CTTGGAGTCTGGGGAGGCAA+AGG 0.540709 8_1:+1466221 Msa1171640:CDS
CTCATTCTTGGAGTCTGGGG+AGG 0.540987 8_1:+1466215 Msa1171640:CDS
ACGTTGCAGATGAGCTTGAA+TGG 0.542906 8_1:+1465585 Msa1171640:intron
GGTATACCGATGGATTGGCC+AGG 0.543252 8_1:-1467386 None:intergenic
ACCTTCCCTGGCCAATCCAT+CGG 0.545441 8_1:+1467380 Msa1171640:CDS
TGGAGTTGCACAAAAGAGAA+AGG 0.548043 8_1:-1464661 None:intergenic
ACCCCTCCATCTGTCTCCGT+CGG 0.548680 8_1:-1465754 None:intergenic
CTTGACCCTACCCAACATCA+AGG 0.551721 8_1:+1466092 Msa1171640:CDS
GTCTGGGGAGGCAAAGGACA+AGG 0.552637 8_1:+1466227 Msa1171640:CDS
CCTACTAGAGAAGATCGTAT+TGG 0.554115 8_1:+1467293 Msa1171640:CDS
AGTTGAAACTGAAGCAGCCA+TGG 0.554794 8_1:-1464697 None:intergenic
TGTTGTACATACCAGGGAGT+TGG 0.556562 8_1:-1467166 None:intergenic
TCAATGTGTTATTAGGTGGA+AGG 0.557881 8_1:+1467968 Msa1171640:three_prime_UTR
CTTCCTTGTTGTACATACCA+GGG 0.559055 8_1:-1467172 None:intergenic
AAAGCTGAATTAGAAACTGT+TGG 0.560874 8_1:-1465624 None:intergenic
ACCGATGGATTGGCCAGGGA+AGG 0.562925 8_1:-1467381 None:intergenic
ACGATGATGAAGTGAGGAAG+TGG 0.566849 8_1:+1467518 Msa1171640:CDS
ACAAGCTTGTCCATAAAAGC+AGG 0.567155 8_1:-1466048 None:intergenic
CCCACCAATGTCCAACTCCC+TGG 0.567626 8_1:+1467155 Msa1171640:CDS
ACAAGACATCACAAGAGGAA+AGG 0.569311 8_1:+1465803 Msa1171640:CDS
CATCTTTAGCGATATTGTCA+TGG 0.579614 8_1:-1467337 None:intergenic
GATGCAGGAGCAGGTCGTAT+GGG 0.579936 8_1:+1467065 Msa1171640:CDS
ATCAGCAATGTTCATGAGTG+TGG 0.586551 8_1:-1467129 None:intergenic
TTGGCAGACAAGTATTTGGA+AGG 0.590350 8_1:+1467694 Msa1171640:CDS
CGTGTGCCCATCATTGTCAC+CGG 0.591897 8_1:+1467203 Msa1171640:CDS
CATACCAGGGAGTTGGACAT+TGG 0.598285 8_1:-1467159 None:intergenic
GACCAACAAGACATCACAAG+AGG 0.598306 8_1:+1465798 Msa1171640:CDS
GCAGGAGCAGGTCGTATGGG+TGG 0.598399 8_1:+1467068 Msa1171640:CDS
TATGCTCCTCTCATCCGTGA+TGG 0.599927 8_1:+1467245 Msa1171640:CDS
AGTGCTGGAGAGCTTGAAAG+TGG 0.600135 8_1:+1466942 Msa1171640:CDS
AACAGACCGACGGAGACAGA+TGG 0.601295 8_1:+1465748 Msa1171640:CDS
CCCCAGACTCCAAGAATGAG+AGG 0.602433 8_1:-1466212 None:intergenic
AATCAAGATGCTATTAAGGA+GGG 0.604002 8_1:+1467736 Msa1171640:CDS
GACCGACGGAGACAGATGGA+GGG 0.605937 8_1:+1465752 Msa1171640:CDS
TTTGGCTGCTCAAACACTGG+TGG 0.609469 8_1:-1467598 None:intergenic
CTCCAGCACTCATCATGATG+GGG 0.610568 8_1:-1466929 None:intergenic
TAATCAAGATGCTATTAAGG+AGG 0.611873 8_1:+1467735 Msa1171640:CDS
ACCGACGGAGACAGATGGAG+GGG 0.612461 8_1:+1465753 Msa1171640:CDS
AAAGAAGCTTGTGAACTCAA+AGG 0.613555 8_1:+1467570 Msa1171640:CDS
CGATGATGAAGTGAGGAAGT+GGG 0.616122 8_1:+1467519 Msa1171640:CDS
AGACCGACGGAGACAGATGG+AGG 0.622548 8_1:+1465751 Msa1171640:CDS
AAGCTTGTGAACTCAAAGGA+AGG 0.624377 8_1:+1467574 Msa1171640:CDS
GTATACCGATGGATTGGCCA+GGG 0.625385 8_1:-1467385 None:intergenic
TAATGAGTTCCAGCATCTTG+TGG 0.630506 8_1:-1465852 None:intergenic
GCAACTACTAGAAGTTGTGA+TGG 0.634634 8_1:+1467927 Msa1171640:three_prime_UTR
CAGGGAGTTGGACATTGGTG+GGG 0.635431 8_1:-1467154 None:intergenic
GAGTGTACGATGATGAAGTG+AGG 0.639625 8_1:+1467512 Msa1171640:CDS
TCGTATGGAGAAGTTCTACT+GGG 0.642236 8_1:+1467268 Msa1171640:CDS
ATACGACCATCACGGATGAG+AGG 0.646877 8_1:-1467251 None:intergenic
GTTGTTGACAGTGTATTGTG+TGG 0.647224 8_1:-1467093 None:intergenic
ACTTCTCCATACGACCATCA+CGG 0.660717 8_1:-1467259 None:intergenic
CTTTAGCGATATTGTCATGG+CGG 0.675976 8_1:-1467334 None:intergenic
GATGACAGTAAACAGACCGA+CGG 0.702756 8_1:+1465738 Msa1171640:CDS
ACTCCCTGGTATGTACAACA+AGG 0.721701 8_1:+1467169 Msa1171640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGTTAAAAGTGATATAT+AGG + chr8_1:1465349-1465368 Msa1171640:intron 15.0%
!!! ACACATTTTATTAATATGTA+TGG + chr8_1:1465934-1465953 Msa1171640:intron 15.0%
!!! ATTTTCATTTTTGTTTTGAT+AGG + chr8_1:1466187-1466206 Msa1171640:intron 15.0%
!! CATAATAGTAACATTAATTA+AGG - chr8_1:1467809-1467828 None:intergenic 15.0%
!! ATTAATGTTACTATTATGTT+TGG + chr8_1:1467811-1467830 Msa1171640:intron 15.0%
!! TAATAAAATTATCTCAAACT+TGG - chr8_1:1468002-1468021 None:intergenic 15.0%
!! GTACAATGTTTAATTAAATT+TGG + chr8_1:1468065-1468084 Msa1171640:three_prime_UTR 15.0%
!! AAAGAAAGAAAAAAAAACAA+GGG - chr8_1:1468118-1468137 None:intergenic 15.0%
!! AAAATTTAAGTCACATGATT+TGG - chr8_1:1464840-1464859 None:intergenic 20.0%
!! TCTAATATGGTATCTATAAA+TGG - chr8_1:1464984-1465003 None:intergenic 20.0%
!! TTTATAGATACCATATTAGA+AGG + chr8_1:1464984-1465003 Msa1171640:intron 20.0%
!! TTATAGATACCATATTAGAA+GGG + chr8_1:1464985-1465004 Msa1171640:intron 20.0%
!! CTTTACATGATTTCTATATA+CGG - chr8_1:1465334-1465353 None:intergenic 20.0%
!! ATAGATAAGTAAGTAAAAAG+AGG + chr8_1:1465508-1465527 Msa1171640:intron 20.0%
!! TAGATAAGTAAGTAAAAAGA+GGG + chr8_1:1465509-1465528 Msa1171640:intron 20.0%
!! TTTATACATGAAATCTCATA+AGG - chr8_1:1466421-1466440 None:intergenic 20.0%
!!! TTTGGTTCAAATAAAAAACT+TGG + chr8_1:1467829-1467848 Msa1171640:intron 20.0%
!! GAAAGAAAGAAAAAAAAACA+AGG - chr8_1:1468119-1468138 None:intergenic 20.0%
! AACAAATGAAAGTAACATAC+TGG - chr8_1:1464741-1464760 None:intergenic 25.0%
! TATATAGTTCACATTCACAT+AGG + chr8_1:1464790-1464809 Msa1171640:intron 25.0%
! TGATACTTAGCTGATATATA+GGG + chr8_1:1464896-1464915 Msa1171640:intron 25.0%
! TATAGGAGTAATAGATTTCA+AGG + chr8_1:1465366-1465385 Msa1171640:intron 25.0%
! AATATCGTGTAAAAATCTAG+TGG - chr8_1:1465460-1465479 None:intergenic 25.0%
! AGATAAGTAAGTAAAAAGAG+GGG + chr8_1:1465510-1465529 Msa1171640:intron 25.0%
! AATTTACATACCTTGATGTT+GGG - chr8_1:1466105-1466124 None:intergenic 25.0%
! AAATTTACATACCTTGATGT+TGG - chr8_1:1466106-1466125 None:intergenic 25.0%
! TAAATTGTCTCTACTATCAT+AGG + chr8_1:1466437-1466456 Msa1171640:intron 25.0%
! AAATTGTCTCTACTATCATA+GGG + chr8_1:1466438-1466457 Msa1171640:intron 25.0%
! ACTTACGACTATAAACTATT+TGG + chr8_1:1466487-1466506 Msa1171640:intron 25.0%
! ATAAGTTCTTTCAACCATTA+CGG + chr8_1:1466566-1466585 Msa1171640:intron 25.0%
! TTATCTATTGACATATACAC+TGG + chr8_1:1466615-1466634 Msa1171640:intron 25.0%
! ACATTAAATAGACACAAGTT+AGG - chr8_1:1466893-1466912 None:intergenic 25.0%
! AAATAGACAGATAGATAGAT+AGG - chr8_1:1467410-1467429 None:intergenic 25.0%
! ATAATAGCTCATAAATGAGA+CGG - chr8_1:1467463-1467482 None:intergenic 25.0%
! AAACTTGGACTAAAAAATCT+TGG + chr8_1:1467844-1467863 Msa1171640:intron 25.0%
! AACTTGGACTAAAAAATCTT+GGG + chr8_1:1467845-1467864 Msa1171640:intron 25.0%
!! AACATTCTCAATGTGTTATT+AGG + chr8_1:1467961-1467980 Msa1171640:three_prime_UTR 25.0%
!!! AAAAAACAAGGGTATCTAAT+AGG - chr8_1:1468107-1468126 None:intergenic 25.0%
! TCATCATATAATTGGGATAA+TGG - chr8_1:1468167-1468186 None:intergenic 25.0%
! CACATGTTCATCATATAATT+GGG - chr8_1:1468174-1468193 None:intergenic 25.0%
! TCACATGTTCATCATATAAT+TGG - chr8_1:1468175-1468194 None:intergenic 25.0%
!!! TTTCTTTTCTCTTGTTTGAT+TGG + chr8_1:1468229-1468248 Msa1171640:three_prime_UTR 25.0%
ATATCAGCTAAGTATCACAT+AGG - chr8_1:1464893-1464912 None:intergenic 30.0%
GTGATACTTAGCTGATATAT+AGG + chr8_1:1464895-1464914 Msa1171640:intron 30.0%
AACTAATCACCCTTCTAATA+TGG - chr8_1:1464997-1465016 None:intergenic 30.0%
ATATTCAAGACCGTTTGAAT+CGG + chr8_1:1465061-1465080 Msa1171640:intron 30.0%
! CTTAAATAAGCCGATTCAAA+CGG - chr8_1:1465074-1465093 None:intergenic 30.0%
ATAAGCTCTCTCAAATACTT+AGG - chr8_1:1465109-1465128 None:intergenic 30.0%
TTTCTATATACGGATCTGTT+TGG - chr8_1:1465324-1465343 None:intergenic 30.0%
AGTAATAGATTTCAAGGAGT+TGG + chr8_1:1465372-1465391 Msa1171640:intron 30.0%
GATAAGTAAGTAAAAAGAGG+GGG + chr8_1:1465511-1465530 Msa1171640:intron 30.0%
AAAGCTGAATTAGAAACTGT+TGG - chr8_1:1465627-1465646 None:intergenic 30.0%
GTTTCTAATTCAGCTTTCTT+CGG + chr8_1:1465630-1465649 Msa1171640:CDS 30.0%
! TTGGTCATCTGAAATATCAT+AGG - chr8_1:1465784-1465803 None:intergenic 30.0%
TCTTATGAGTACATTAGTAC+TGG + chr8_1:1465888-1465907 Msa1171640:CDS 30.0%
! TCATCTAAAACTTGTGATGA+TGG + chr8_1:1465977-1465996 Msa1171640:intron 30.0%
TGTAGGATTTCAAAGTTATG+AGG - chr8_1:1466380-1466399 None:intergenic 30.0%
CTTGTTTAGTAATCACTTGT+AGG - chr8_1:1466397-1466416 None:intergenic 30.0%
AATTGTCTCTACTATCATAG+GGG + chr8_1:1466439-1466458 Msa1171640:intron 30.0%
ATATGTCCATAAGCTGTATT+CGG - chr8_1:1466532-1466551 None:intergenic 30.0%
TAGCGTATAAAAGCAGTTTA+TGG + chr8_1:1466711-1466730 Msa1171640:intron 30.0%
ATGAAATGAGCACTTGAATT+AGG - chr8_1:1466833-1466852 None:intergenic 30.0%
GATAATGTAACATGCTTCTT+TGG - chr8_1:1466865-1466884 None:intergenic 30.0%
!! AAGTGTTGAGAAATCATTAC+CGG - chr8_1:1467225-1467244 None:intergenic 30.0%
GCTATTATTGCAGATTTCTT+CGG + chr8_1:1467475-1467494 Msa1171640:intron 30.0%
TGCTAATCAAGATGCTATTA+AGG + chr8_1:1467732-1467751 Msa1171640:CDS 30.0%
TAATCAAGATGCTATTAAGG+AGG + chr8_1:1467735-1467754 Msa1171640:CDS 30.0%
AATCAAGATGCTATTAAGGA+GGG + chr8_1:1467736-1467755 Msa1171640:CDS 30.0%
! ATTCTCAATGTGTTATTAGG+TGG + chr8_1:1467964-1467983 Msa1171640:three_prime_UTR 30.0%
TCTAATAGGTAGAAAGAGAA+AGG - chr8_1:1468093-1468112 None:intergenic 30.0%
! TTTTGTGTTTGGAAGTCTTG+CGG - chr8_1:1465676-1465695 None:intergenic 35.0%
!! AAAAACTTTGAAGCTTCCTG+AGG - chr8_1:1465703-1465722 None:intergenic 35.0%
!!! ACTTGTGATGATGGTGTTTT+AGG + chr8_1:1465986-1466005 Msa1171640:intron 35.0%
GTACAACTTTGACAACACTA+TGG + chr8_1:1466008-1466027 Msa1171640:CDS 35.0%
AACTTTGACAACACTATGGA+TGG + chr8_1:1466012-1466031 Msa1171640:CDS 35.0%
!! TTACATTGCTCCTGCTTTTA+TGG + chr8_1:1466038-1466057 Msa1171640:CDS 35.0%
TGCTATGACTTTAGCAGAAT+TGG + chr8_1:1466150-1466169 Msa1171640:intron 35.0%
GCTATGACTTTAGCAGAATT+GGG + chr8_1:1466151-1466170 Msa1171640:intron 35.0%
AATTAGCCGAATACAGCTTA+TGG + chr8_1:1466523-1466542 Msa1171640:intron 35.0%
TAATCGAAACATGTCCGTAA+TGG - chr8_1:1466583-1466602 None:intergenic 35.0%
ATACACTGGTGAGACTATTT+AGG + chr8_1:1466629-1466648 Msa1171640:intron 35.0%
!! ACTGCTTTTATACGCTATCT+TGG - chr8_1:1466708-1466727 None:intergenic 35.0%
CTGTAAGCTCTTGATAAACA+TGG + chr8_1:1466807-1466826 Msa1171640:intron 35.0%
ATACACTGTCAACAACCAAA+TGG + chr8_1:1467100-1467119 Msa1171640:CDS 35.0%
CATCTTTAGCGATATTGTCA+TGG - chr8_1:1467340-1467359 None:intergenic 35.0%
TAGATAGATAGGTATACCGA+TGG - chr8_1:1467399-1467418 None:intergenic 35.0%
!! GGAGTTGGTATTGAAACAAT+TGG + chr8_1:1467547-1467566 Msa1171640:CDS 35.0%
AAAGAAGCTTGTGAACTCAA+AGG + chr8_1:1467570-1467589 Msa1171640:CDS 35.0%
!! AGCTTTTCTAGTGTCATCTT+TGG - chr8_1:1467619-1467638 None:intergenic 35.0%
GATGACACTAGAAAAGCTAT+TGG + chr8_1:1467621-1467640 Msa1171640:CDS 35.0%
CTAGAAAAGCTATTGGAGTA+TGG + chr8_1:1467628-1467647 Msa1171640:CDS 35.0%
! TCTAGTTTCTCCAATGATCT+TGG + chr8_1:1467764-1467783 Msa1171640:exon 35.0%
TTACAAACCTCCAAGATCAT+TGG - chr8_1:1467777-1467796 None:intergenic 35.0%
! TCAATGTGTTATTAGGTGGA+AGG + chr8_1:1467968-1467987 Msa1171640:three_prime_UTR 35.0%
TGGAGTTGCACAAAAGAGAA+AGG - chr8_1:1464664-1464683 None:intergenic 40.0%
! ATTTCAAGGAGTTGGTCTAG+TGG + chr8_1:1465380-1465399 Msa1171640:intron 40.0%
AAGTAAGTAAAAAGAGGGGG+AGG + chr8_1:1465514-1465533 Msa1171640:intron 40.0%
TTGAATGGCTCAGTAGTATC+TGG + chr8_1:1465600-1465619 Msa1171640:CDS 40.0%
! CCTGAGGAAACTTTTGTGTT+TGG - chr8_1:1465687-1465706 None:intergenic 40.0%
CCAAACACAAAAGTTTCCTC+AGG + chr8_1:1465684-1465703 Msa1171640:CDS 40.0%
TTCCTCTTGTGATGTCTTGT+TGG - chr8_1:1465803-1465822 None:intergenic 40.0%
ACAAGACATCACAAGAGGAA+AGG + chr8_1:1465803-1465822 Msa1171640:CDS 40.0%
CAAGACATCACAAGAGGAAA+GGG + chr8_1:1465804-1465823 Msa1171640:CDS 40.0%
TAATGAGTTCCAGCATCTTG+TGG - chr8_1:1465855-1465874 None:intergenic 40.0%
ACAAGCTTGTCCATAAAAGC+AGG - chr8_1:1466051-1466070 None:intergenic 40.0%
ACATACCTTGATGTTGGGTA+GGG - chr8_1:1466100-1466119 None:intergenic 40.0%
TACATACCTTGATGTTGGGT+AGG - chr8_1:1466101-1466120 None:intergenic 40.0%
TGATAGGTTCCTCTCATTCT+TGG + chr8_1:1466203-1466222 Msa1171640:intron 40.0%
AACTCACTTGATTCCCATCT+TGG - chr8_1:1466282-1466301 None:intergenic 40.0%
AAGCATGTTGTGCATGGAAT+TGG - chr8_1:1466326-1466345 None:intergenic 40.0%
! ACTAGAAAGCATGTTGTGCA+TGG - chr8_1:1466332-1466351 None:intergenic 40.0%
! CTCTGTCTGATCAATTTTGC+TGG - chr8_1:1466981-1467000 None:intergenic 40.0%
! TGATAATGTCAGAGGCTTCA+CGG - chr8_1:1467007-1467026 None:intergenic 40.0%
! TCCTTTCCTGATAATGTCAG+AGG - chr8_1:1467015-1467034 None:intergenic 40.0%
GTTGTTGACAGTGTATTGTG+TGG - chr8_1:1467096-1467115 None:intergenic 40.0%
ATCAGCAATGTTCATGAGTG+TGG - chr8_1:1467132-1467151 None:intergenic 40.0%
! CTTCCTTGTTGTACATACCA+GGG - chr8_1:1467175-1467194 None:intergenic 40.0%
! TCTTCCTTGTTGTACATACC+AGG - chr8_1:1467176-1467195 None:intergenic 40.0%
TCATTACCGGTGACAATGAT+GGG - chr8_1:1467212-1467231 None:intergenic 40.0%
ATCATTACCGGTGACAATGA+TGG - chr8_1:1467213-1467232 None:intergenic 40.0%
TCGTATGGAGAAGTTCTACT+GGG + chr8_1:1467268-1467287 Msa1171640:CDS 40.0%
CCAATACGATCTTCTCTAGT+AGG - chr8_1:1467296-1467315 None:intergenic 40.0%
CCTACTAGAGAAGATCGTAT+TGG + chr8_1:1467293-1467312 Msa1171640:CDS 40.0%
CTTTAGCGATATTGTCATGG+CGG - chr8_1:1467337-1467356 None:intergenic 40.0%
AGATAGGTATACCGATGGAT+TGG - chr8_1:1467394-1467413 None:intergenic 40.0%
! AGATTTCTTCGGTGCATTGA+GGG + chr8_1:1467486-1467505 Msa1171640:intron 40.0%
AAGCTTGTGAACTCAAAGGA+AGG + chr8_1:1467574-1467593 Msa1171640:CDS 40.0%
GAATGTGAAGAGAGTCCAAT+TGG + chr8_1:1467675-1467694 Msa1171640:CDS 40.0%
CCAAATACTTGTCTGCCAAT+TGG - chr8_1:1467693-1467712 None:intergenic 40.0%
CCAATTGGCAGACAAGTATT+TGG + chr8_1:1467690-1467709 Msa1171640:CDS 40.0%
TTGGCAGACAAGTATTTGGA+AGG + chr8_1:1467694-1467713 Msa1171640:CDS 40.0%
AGTTTCTCCAATGATCTTGG+AGG + chr8_1:1467767-1467786 Msa1171640:exon 40.0%
AGTAGTTGCATCGAAGTTTG+AGG - chr8_1:1467916-1467935 None:intergenic 40.0%
GCAACTACTAGAAGTTGTGA+TGG + chr8_1:1467927-1467946 Msa1171640:three_prime_UTR 40.0%
TCCAATCAGTGCAGTATCCA+TGG + chr8_1:1464680-1464699 Msa1171640:exon 45.0%
AGTTGAAACTGAAGCAGCCA+TGG - chr8_1:1464700-1464719 None:intergenic 45.0%
GCTGCTTCAGTTTCAACTGT+TGG + chr8_1:1464702-1464721 Msa1171640:CDS 45.0%
ACGTTGCAGATGAGCTTGAA+TGG + chr8_1:1465585-1465604 Msa1171640:intron 45.0%
GATGACAGTAAACAGACCGA+CGG + chr8_1:1465738-1465757 Msa1171640:CDS 45.0%
GACCAACAAGACATCACAAG+AGG + chr8_1:1465798-1465817 Msa1171640:CDS 45.0%
CATCACAAGAGGAAAGGGTT+TGG + chr8_1:1465809-1465828 Msa1171640:CDS 45.0%
GGGTAGGGTCAAGAAATTCT+TGG - chr8_1:1466085-1466104 None:intergenic 45.0%
TTCCTCTCATTCTTGGAGTC+TGG + chr8_1:1466210-1466229 Msa1171640:CDS 45.0%
TCCTCTCATTCTTGGAGTCT+GGG + chr8_1:1466211-1466230 Msa1171640:CDS 45.0%
TGAGCTTGTCTTTGCCAAGA+TGG + chr8_1:1466265-1466284 Msa1171640:CDS 45.0%
GAGCTTGTCTTTGCCAAGAT+GGG + chr8_1:1466266-1466285 Msa1171640:CDS 45.0%
! CGCTATCTTGGAGTGCTTAT+TGG - chr8_1:1466696-1466715 None:intergenic 45.0%
TCTCCAGCACTCATCATGAT+GGG - chr8_1:1466933-1466952 None:intergenic 45.0%
GAGCTTGAAAGTGGAAATGC+AGG + chr8_1:1466951-1466970 Msa1171640:CDS 45.0%
GTGAAGCCTCTGACATTATC+AGG + chr8_1:1467006-1467025 Msa1171640:CDS 45.0%
GCCTCTGACATTATCAGGAA+AGG + chr8_1:1467011-1467030 Msa1171640:CDS 45.0%
TTCATCAACGATCTCGATGC+AGG + chr8_1:1467050-1467069 Msa1171640:CDS 45.0%
TGAGTGTGGCATTCACCATT+TGG - chr8_1:1467118-1467137 None:intergenic 45.0%
TGTTGTACATACCAGGGAGT+TGG - chr8_1:1467169-1467188 None:intergenic 45.0%
ACTCCCTGGTATGTACAACA+AGG + chr8_1:1467169-1467188 Msa1171640:CDS 45.0%
ACTTCTCCATACGACCATCA+CGG - chr8_1:1467262-1467281 None:intergenic 45.0%
GTCGTATGGAGAAGTTCTAC+TGG + chr8_1:1467267-1467286 Msa1171640:CDS 45.0%
! GATCGTATTGGTGTCTGCAT+TGG + chr8_1:1467305-1467324 Msa1171640:CDS 45.0%
AAGATTGTTGACACCTTCCC+TGG + chr8_1:1467368-1467387 Msa1171640:CDS 45.0%
CTGACAAACAGACAGACAGA+CGG - chr8_1:1467439-1467458 None:intergenic 45.0%
! CAGATTTCTTCGGTGCATTG+AGG + chr8_1:1467485-1467504 Msa1171640:intron 45.0%
GAGTGTACGATGATGAAGTG+AGG + chr8_1:1467512-1467531 Msa1171640:CDS 45.0%
ACGATGATGAAGTGAGGAAG+TGG + chr8_1:1467518-1467537 Msa1171640:CDS 45.0%
CGATGATGAAGTGAGGAAGT+GGG + chr8_1:1467519-1467538 Msa1171640:CDS 45.0%
ATCTTTGGCTGCTCAAACAC+TGG - chr8_1:1467604-1467623 None:intergenic 45.0%
!! TATTTGGAAGGTGCTGCTCT+TGG + chr8_1:1467706-1467725 Msa1171640:CDS 45.0%
!! ATCTTGGGTTTGTTGTGTGC+AGG + chr8_1:1467860-1467879 Msa1171640:intron 45.0%
!!! TTTTATTATATTTTTCTTTT+TGG + chr8_1:1468014-1468033 Msa1171640:three_prime_UTR 5.0%
! GCCATGGATACTGCACTGAT+TGG - chr8_1:1464684-1464703 None:intergenic 50.0%
TTCCAAGCACCACAAGATGC+TGG + chr8_1:1465843-1465862 Msa1171640:CDS 50.0%
TTCCAGCATCTTGTGGTGCT+TGG - chr8_1:1465848-1465867 None:intergenic 50.0%
CTTGACCCTACCCAACATCA+AGG + chr8_1:1466092-1466111 Msa1171640:CDS 50.0%
CCTCTCATTCTTGGAGTCTG+GGG + chr8_1:1466212-1466231 Msa1171640:CDS 50.0%
TGGCAAAGACAAGCTCAGCT+TGG - chr8_1:1466262-1466281 None:intergenic 50.0%
CTCCAGCACTCATCATGATG+GGG - chr8_1:1466932-1466951 None:intergenic 50.0%
CTCTCCAGCACTCATCATGA+TGG - chr8_1:1466934-1466953 None:intergenic 50.0%
! AGTGCTGGAGAGCTTGAAAG+TGG + chr8_1:1466942-1466961 Msa1171640:CDS 50.0%
CATACCAGGGAGTTGGACAT+TGG - chr8_1:1467162-1467181 None:intergenic 50.0%
TATGCTCCTCTCATCCGTGA+TGG + chr8_1:1467245-1467264 Msa1171640:CDS 50.0%
ATACGACCATCACGGATGAG+AGG - chr8_1:1467254-1467273 None:intergenic 50.0%
TCTCATCCGTGATGGTCGTA+TGG + chr8_1:1467253-1467272 Msa1171640:CDS 50.0%
!! GTATACCGATGGATTGGCCA+GGG - chr8_1:1467388-1467407 None:intergenic 50.0%
! GAAGTGAGGAAGTGGGTTTC+TGG + chr8_1:1467526-1467545 Msa1171640:CDS 50.0%
!! AGGAAGTGGGTTTCTGGAGT+TGG + chr8_1:1467532-1467551 Msa1171640:CDS 50.0%
TTTGGCTGCTCAAACACTGG+TGG - chr8_1:1467601-1467620 None:intergenic 50.0%
AACAGACCGACGGAGACAGA+TGG + chr8_1:1465748-1465767 Msa1171640:CDS 55.0%
CCCCAGACTCCAAGAATGAG+AGG - chr8_1:1466215-1466234 None:intergenic 55.0%
CTCATTCTTGGAGTCTGGGG+AGG + chr8_1:1466215-1466234 Msa1171640:CDS 55.0%
AGCCCCATCATGATGAGTGC+TGG + chr8_1:1466927-1466946 Msa1171640:intron 55.0%
AACGATCTCGATGCAGGAGC+AGG + chr8_1:1467056-1467075 Msa1171640:CDS 55.0%
GATGCAGGAGCAGGTCGTAT+GGG + chr8_1:1467065-1467084 Msa1171640:CDS 55.0%
CAGGGAGTTGGACATTGGTG+GGG - chr8_1:1467157-1467176 None:intergenic 55.0%
CCAGGGAGTTGGACATTGGT+GGG - chr8_1:1467158-1467177 None:intergenic 55.0%
ACCAGGGAGTTGGACATTGG+TGG - chr8_1:1467159-1467178 None:intergenic 55.0%
CGTGTGCCCATCATTGTCAC+CGG + chr8_1:1467203-1467222 Msa1171640:CDS 55.0%
ACCTTCCCTGGCCAATCCAT+CGG + chr8_1:1467380-1467399 Msa1171640:CDS 55.0%
!! GGTATACCGATGGATTGGCC+AGG - chr8_1:1467389-1467408 None:intergenic 55.0%
AGACCGACGGAGACAGATGG+AGG + chr8_1:1465751-1465770 Msa1171640:CDS 60.0%
GACCGACGGAGACAGATGGA+GGG + chr8_1:1465752-1465771 Msa1171640:CDS 60.0%
ACCGACGGAGACAGATGGAG+GGG + chr8_1:1465753-1465772 Msa1171640:CDS 60.0%
ACCCCTCCATCTGTCTCCGT+CGG - chr8_1:1465757-1465776 None:intergenic 60.0%
CGGAGACAGATGGAGGGGTT+TGG + chr8_1:1465758-1465777 Msa1171640:CDS 60.0%
CTTGGAGTCTGGGGAGGCAA+AGG + chr8_1:1466221-1466240 Msa1171640:CDS 60.0%
GTCTGGGGAGGCAAAGGACA+AGG + chr8_1:1466227-1466246 Msa1171640:CDS 60.0%
CGATGCAGGAGCAGGTCGTA+TGG + chr8_1:1467064-1467083 Msa1171640:CDS 60.0%
CCCACCAATGTCCAACTCCC+TGG + chr8_1:1467155-1467174 Msa1171640:CDS 60.0%
!! ACCGATGGATTGGCCAGGGA+AGG - chr8_1:1467384-1467403 None:intergenic 60.0%
GCAGGAGCAGGTCGTATGGG+TGG + chr8_1:1467068-1467087 Msa1171640:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 1464631 1468297 1464631 ID=Msa1171640;Name=Msa1171640
chr8_1 mRNA 1464631 1468297 1464631 ID=Msa1171640-mRNA-1;Parent=Msa1171640;Name=Msa1171640-mRNA-1;_AED=0.02;_eAED=0.02;_QI=68|1|1|1|0.83|0.71|7|436|437
chr8_1 exon 1464631 1464740 1464631 ID=Msa1171640-mRNA-1:exon:430;Parent=Msa1171640-mRNA-1
chr8_1 exon 1465594 1465919 1465594 ID=Msa1171640-mRNA-1:exon:431;Parent=Msa1171640-mRNA-1
chr8_1 exon 1466008 1466113 1466008 ID=Msa1171640-mRNA-1:exon:432;Parent=Msa1171640-mRNA-1
chr8_1 exon 1466209 1466294 1466209 ID=Msa1171640-mRNA-1:exon:433;Parent=Msa1171640-mRNA-1
chr8_1 exon 1466929 1467401 1466929 ID=Msa1171640-mRNA-1:exon:434;Parent=Msa1171640-mRNA-1
chr8_1 exon 1467488 1467788 1467488 ID=Msa1171640-mRNA-1:exon:435;Parent=Msa1171640-mRNA-1
chr8_1 exon 1467882 1468297 1467882 ID=Msa1171640-mRNA-1:exon:436;Parent=Msa1171640-mRNA-1
chr8_1 five_prime_UTR 1464631 1464698 1464631 ID=Msa1171640-mRNA-1:five_prime_utr;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1464699 1464740 1464699 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1465594 1465919 1465594 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1466008 1466113 1466008 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1466209 1466294 1466209 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1466929 1467401 1466929 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 CDS 1467488 1467768 1467488 ID=Msa1171640-mRNA-1:cds;Parent=Msa1171640-mRNA-1
chr8_1 three_prime_UTR 1467769 1467788 1467769 ID=Msa1171640-mRNA-1:three_prime_utr;Parent=Msa1171640-mRNA-1
chr8_1 three_prime_UTR 1467882 1468297 1467882 ID=Msa1171640-mRNA-1:three_prime_utr;Parent=Msa1171640-mRNA-1
Gene Sequence

>Msa1171640

ATGGCTGCTTCAGTTTCAACTGTTGGAGCTGTCAAAACTCCAATGAGCTTGAATGGCTCAGTAGTATCTGGACCAACAGTTTCTAATTCAGCTTTCTTCGGAACAAGCTTGAAGAAGCTTACCGCAAGACTTCCAAACACAAAAGTTTCCTCAGGAAGCTTCAAAGTTTTTGCTGCTAAAGAGATCGATGACAGTAAACAGACCGACGGAGACAGATGGAGGGGTTTGGCCTATGATATTTCAGATGACCAACAAGACATCACAAGAGGAAAGGGTTTGGTTGATTCAGTTTTCCAAGCACCACAAGATGCTGGAACTCATTATGCAGTCATGAGTTCTTATGAGTACATTAGTACTGGACTTAAACAGTACAACTTTGACAACACTATGGATGGATTTTACATTGCTCCTGCTTTTATGGACAAGCTTGTTGTTCACATCACCAAGAATTTCTTGACCCTACCCAACATCAAGGTTCCTCTCATTCTTGGAGTCTGGGGAGGCAAAGGACAAGGAAAATCTTTCCAAGCTGAGCTTGTCTTTGCCAAGATGGGAATCAACCCCATCATGATGAGTGCTGGAGAGCTTGAAAGTGGAAATGCAGGAGAGCCAGCAAAATTGATCAGACAGAGATACCGTGAAGCCTCTGACATTATCAGGAAAGGAAAGATGTGTTGTCTGTTCATCAACGATCTCGATGCAGGAGCAGGTCGTATGGGTGGAACCACACAATACACTGTCAACAACCAAATGGTGAATGCCACACTCATGAACATTGCTGATAACCCCACCAATGTCCAACTCCCTGGTATGTACAACAAGGAAGACAATGCTCGTGTGCCCATCATTGTCACCGGTAATGATTTCTCAACACTTTATGCTCCTCTCATCCGTGATGGTCGTATGGAGAAGTTCTACTGGGCTCCTACTAGAGAAGATCGTATTGGTGTCTGCATTGGAATTTTCCGCCATGACAATATCGCTAAAGATGACATTGTCAAGATTGTTGACACCTTCCCTGGCCAATCCATCGATTTCTTCGGTGCATTGAGGGCTAGAGTGTACGATGATGAAGTGAGGAAGTGGGTTTCTGGAGTTGGTATTGAAACAATTGGAAAGAAGCTTGTGAACTCAAAGGAAGGACCACCAGTGTTTGAGCAGCCAAAGATGACACTAGAAAAGCTATTGGAGTATGGTAACATGCTTGTCTCAGAACAAGAGAATGTGAAGAGAGTCCAATTGGCAGACAAGTATTTGGAAGGTGCTGCTCTTGGTGATGCTAATCAAGATGCTATTAAGGAGGGAAAATTCTAG
Protein sequence

>Msa1171640

MAASVSTVGAVKTPMSLNGSVVSGPTVSNSAFFGTSLKKLTARLPNTKVSSGSFKVFAAKEIDDSKQTDGDRWRGLAYDISDDQQDITRGKGLVDSVFQAPQDAGTHYAVMSSYEYISTGLKQYNFDNTMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGVWGGKGQGKSFQAELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREASDIIRKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCIGIFRHDNIAKDDIVKIVDTFPGQSIDFFGALRARVYDDEVRKWVSGVGIETIGKKLVNSKEGPPVFEQPKMTLEKLLEYGNMLVSEQENVKRVQLADKYLEGAALGDANQDAIKEGKF*