AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421520 MtrunA17_Chr7g0262831 98.919 185 2 0 1 185 1 185 1.02e-129 369
Msa1421520 MtrunA17_Chr7g0262841 98.400 125 2 0 242 366 8 132 1.01e-85 270
Msa1421520 MtrunA17_Chr2g0327991 42.286 350 193 4 14 356 50 397 3.48e-70 234
Msa1421520 MtrunA17_Chr7g0244781 95.370 108 5 0 61 168 17 124 5.47e-66 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421520 AT3G09450.1 40.113 354 195 6 11 359 2 343 6.54e-64 217
Msa1421520 AT2G28780.1 37.607 351 206 8 14 356 10 355 7.13e-59 204

Find 68 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTAATGTAACCCTTTAT+AGG 0.227954 tig0025135:+3246 None:intergenic
CGTCAACAAGTGTGTCGATT+TGG 0.272339 tig0025135:+3014 None:intergenic
ATATAGCCCTCAACCTATAA+AGG 0.287495 tig0025135:-3259 Msa1421520:CDS
TTATAGGTTGAGGGCTATAT+AGG 0.300994 tig0025135:+3262 None:intergenic
AGAAGAGAAACTGCAAGATT+TGG 0.304292 tig0025135:-301 Msa1421520:CDS
TGGAGCTATAGCTTCTGTTC+TGG 0.352610 tig0025135:-2881 Msa1421520:CDS
CATACAGTTGCCGAAGTTAT+CGG 0.375339 tig0025135:+3080 None:intergenic
TCCTGTTGTGGAGAGGTACT+TGG 0.379008 tig0025135:+541 None:intergenic
GTCAACAAGTGTGTCGATTT+GGG 0.380204 tig0025135:+3015 None:intergenic
CCAGTGTAGTTGTGATACTA+TGG 0.386508 tig0025135:+507 None:intergenic
ACCTTAGCATGCAGCATAGT+TGG 0.394869 tig0025135:-3294 Msa1421520:CDS
CTGTATGGCTGCGCTGGCAG+TGG 0.396194 tig0025135:-3064 Msa1421520:CDS
TATAGGTTGAGGGCTATATA+GGG 0.405330 tig0025135:+3263 None:intergenic
ATGTAACCCTTTATAGGTTG+AGG 0.416760 tig0025135:+3252 None:intergenic
GTGACGCCGTTAGAGGTTGT+TGG 0.421688 tig0025135:-3167 Msa1421520:CDS
ACCACCTTCTTTAAAATGTC+AGG 0.424833 tig0025135:-3384 Msa1421520:exon
ACACTTGGTGACGCCGTTAG+AGG 0.424878 tig0025135:-3174 Msa1421520:CDS
ATACAGTTGCCGAAGTTATC+GGG 0.436138 tig0025135:+3081 None:intergenic
CGACACACTTGTTGACGAAA+AGG 0.437706 tig0025135:-3008 Msa1421520:CDS
GCCAAGTACCTCTCCACAAC+AGG 0.438296 tig0025135:-542 Msa1421520:CDS
TGTAACCCTTTATAGGTTGA+GGG 0.455045 tig0025135:+3253 None:intergenic
CGATAACTTCGGCAACTGTA+TGG 0.455133 tig0025135:-3079 Msa1421520:CDS
TGCTGCCCCTGCCCTATCCT+CGG 0.456066 tig0025135:-2852 Msa1421520:CDS
TTGACGAAAAGGATTGCATA+TGG 0.458789 tig0025135:-2997 Msa1421520:CDS
GTGGCTTGTTCCACTGCACT+TGG 0.469029 tig0025135:-2901 Msa1421520:CDS
TATAGCCCTCAACCTATAAA+GGG 0.471608 tig0025135:-3258 Msa1421520:CDS
CGGCAACTGTATGGCTGCGC+TGG 0.476765 tig0025135:-3070 Msa1421520:CDS
ATGTATTCTCAATGTATAGT+TGG 0.481404 tig0025135:+639 None:intergenic
GAGATTAAATTGCAATATAG+AGG 0.485208 tig0025135:-612 Msa1421520:CDS
GATTTGGAAATACCAATAAG+AGG 0.489960 tig0025135:-285 Msa1421520:CDS
TGAGTTGTCTGAGGCTGAGA+TGG 0.496938 tig0025135:+589 None:intergenic
AGTGGCTACCGGTGCATTTG+TGG 0.499348 tig0025135:-3046 Msa1421520:CDS
ATGTAAGCAAACCGAGGATA+GGG 0.500818 tig0025135:+2841 None:intergenic
TATGAGAATTGTTGTGACAT+AGG 0.502472 tig0025135:+3212 None:intergenic
ACAAACAGGATGGCATGCAT+TGG 0.502802 tig0025135:-368 Msa1421520:intron
TCCTCGGTTTGCTTACATTG+AGG 0.507319 tig0025135:-2836 Msa1421520:intron
CAAACAGGATGGCATGCATT+GGG 0.519234 tig0025135:-367 Msa1421520:intron
AATGTAAGCAAACCGAGGAT+AGG 0.523686 tig0025135:+2840 None:intergenic
TCAGACAACTCAACTGCTGT+TGG 0.529606 tig0025135:-578 Msa1421520:CDS
TCCTGTTGGTGTTATTGATG+AGG 0.529816 tig0025135:-229 Msa1421520:CDS
ATAGGGCAGGGGCAGCAACA+TGG 0.541183 tig0025135:+2858 None:intergenic
GAGAAGCTTGGCTCCTGTTG+TGG 0.542438 tig0025135:+529 None:intergenic
TGTGATACTATGGAGAAGCT+TGG 0.546804 tig0025135:+517 None:intergenic
GAGTTGTTTCGAACTCGTCT+TGG 0.547127 tig0025135:-3333 Msa1421520:CDS
ACCAACTATGCTGCATGCTA+AGG 0.552023 tig0025135:+3293 None:intergenic
AAGCAAACCGAGGATAGGGC+AGG 0.561371 tig0025135:+2845 None:intergenic
GTTATACATGGTGCACAAGA+AGG 0.578194 tig0025135:-2946 Msa1421520:CDS
GTTGTGACATAGGAAATAGA+TGG 0.579686 tig0025135:+3222 None:intergenic
ATTCTCATAGTGTTGTCTGA+TGG 0.580392 tig0025135:-3198 Msa1421520:CDS
AACATGCCAACAACCTCTAA+CGG 0.582906 tig0025135:+3161 None:intergenic
AGCAAACCGAGGATAGGGCA+GGG 0.590590 tig0025135:+2846 None:intergenic
GCAAACCGAGGATAGGGCAG+GGG 0.590989 tig0025135:+2847 None:intergenic
ATTTGGAAATACCAATAAGA+GGG 0.595637 tig0025135:-284 Msa1421520:CDS
CAGCGCCACCACAAATGCAC+CGG 0.599257 tig0025135:+3038 None:intergenic
AGCTATAGCTCCAAGTGCAG+TGG 0.600339 tig0025135:+2891 None:intergenic
GGAAATACCAATAAGAGGGA+TGG 0.600471 tig0025135:-280 Msa1421520:CDS
GTTATACGACCCGATAACTT+CGG 0.617939 tig0025135:-3090 Msa1421520:CDS
CCATAGTATCACAACTACAC+TGG 0.621214 tig0025135:-507 Msa1421520:intron
TATGTCAGCACAGTTATACA+TGG 0.621877 tig0025135:-2958 Msa1421520:CDS
GGCTACCGGTGCATTTGTGG+TGG 0.628149 tig0025135:-3043 Msa1421520:CDS
GGCTACGCATTCAATTCATG+TGG 0.631946 tig0025135:-2920 Msa1421520:CDS
GCTTGGCTCCTGTTGTGGAG+AGG 0.637405 tig0025135:+534 None:intergenic
ACATGGTGCACAAGAAGGAG+TGG 0.641976 tig0025135:-2941 Msa1421520:CDS
GTTATTGATGAGGAGCTAAG+AGG 0.643274 tig0025135:-219 Msa1421520:CDS
ATGGACATTGCTCTATCGAG+TGG 0.643627 tig0025135:-261 Msa1421520:CDS
AACAGCAGTTGAGTTGTCTG+AGG 0.659969 tig0025135:+580 None:intergenic
GTGTTGTCTGATGGAACACT+TGG 0.689623 tig0025135:-3189 Msa1421520:CDS
ACCTCAATGTAAGCAAACCG+AGG 0.693462 tig0025135:+2835 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACACAAAAAAAAAAAAAAA+AGG + tig0025135:1016-1035 None:intergenic 10.0%
!!! AAATAATACCTTTAAAAAAA+AGG - tig0025135:1425-1444 Msa1421520:intron 10.0%
!!! AATAATACCTTTAAAAAAAA+GGG - tig0025135:1426-1445 Msa1421520:intron 10.0%
!!! TATTTTACAAAAATTATTGA+AGG + tig0025135:1622-1641 None:intergenic 10.0%
!!! ATTCATTTTAATGATTAAAT+TGG + tig0025135:1654-1673 None:intergenic 10.0%
!! TATATTTAAAAACTTAAATC+TGG - tig0025135:1754-1773 Msa1421520:intron 10.0%
!!! TTTTTTTTTTTTAAACAAAC+AGG - tig0025135:3031-3050 Msa1421520:CDS 10.0%
!!! TTTTTTTTTTTTTGTTGAAG+TGG - tig0025135:667-686 Msa1421520:intron 15.0%
!!! AAAAAAAAGTTTTTTTGAAC+AGG + tig0025135:820-839 None:intergenic 15.0%
!! ATTGAATAAAAATGATCTTA+TGG + tig0025135:942-961 None:intergenic 15.0%
!!! ATAATACCTTTAAAAAAAAG+GGG - tig0025135:1427-1446 Msa1421520:intron 15.0%
!!! TAGATTTAATTTGAGAAAAT+AGG - tig0025135:2263-2282 Msa1421520:intron 15.0%
!!! TTTTTATTTATATTAGTGAC+CGG - tig0025135:2488-2507 Msa1421520:intron 15.0%
!! AATTTAATGTAACCCTTTAT+AGG + tig0025135:170-189 None:intergenic 20.0%
!!! AATCACAATTTAGAATAAAC+AGG - tig0025135:712-731 Msa1421520:intron 20.0%
!! TTAAAATGAGAAGCTATTTA+CGG - tig0025135:908-927 Msa1421520:intron 20.0%
!!! ATTATTGCTATTGTTATGAT+CGG - tig0025135:1294-1313 Msa1421520:intron 20.0%
!!! TAATACCTTTAAAAAAAAGG+GGG - tig0025135:1428-1447 Msa1421520:intron 20.0%
!!! AAAAACTTAAATCTGGAATT+TGG - tig0025135:1761-1780 Msa1421520:intron 20.0%
!!! TTAGTTGGATTGATTTTAAT+CGG + tig0025135:1969-1988 None:intergenic 20.0%
!!! AAAATCTTTGTTTTTTTGAC+CGG - tig0025135:2047-2066 Msa1421520:intron 20.0%
!!! TAAGATTTTTTCTTACTTTC+CGG + tig0025135:2069-2088 None:intergenic 20.0%
!! CAATGCCTAATATAATAATT+TGG + tig0025135:2109-2128 None:intergenic 20.0%
!! AATTGAATGTATCTAAAAAG+TGG - tig0025135:2464-2483 Msa1421520:intron 20.0%
!! TAAATGACATAACACATTAT+TGG + tig0025135:2630-2649 None:intergenic 20.0%
!!! AAAGTGTGTTTTTAATATCT+TGG + tig0025135:2720-2739 None:intergenic 20.0%
!!! TTTTTTTTAAACAAACAGGA+TGG - tig0025135:3035-3054 Msa1421520:CDS 20.0%
!! AGTATAAAAAGAATGATGTT+GGG + tig0025135:3361-3380 None:intergenic 20.0%
! AGAAAAAATCATAACCTGAA+TGG + tig0025135:288-307 None:intergenic 25.0%
! TTTATGACACTTTCAAAATG+TGG - tig0025135:1402-1421 Msa1421520:intron 25.0%
!!! TTATTCCCCCTTTTTTTTAA+AGG + tig0025135:1436-1455 None:intergenic 25.0%
! AGGGGGAATAATAATATATT+TGG - tig0025135:1445-1464 Msa1421520:intron 25.0%
! AATTATGTCAATTCAAACGT+TGG + tig0025135:1714-1733 None:intergenic 25.0%
! GAATTATTATGCTAAGTGAT+GGG + tig0025135:1919-1938 None:intergenic 25.0%
! AGAAACAAATTGACATTAGT+TGG + tig0025135:1984-2003 None:intergenic 25.0%
! TTACTTGCATCAAATTATAC+AGG + tig0025135:2025-2044 None:intergenic 25.0%
! CGCAACCAAATTATTATATT+AGG - tig0025135:2101-2120 Msa1421520:intron 25.0%
! AACTTCTGTAATACTATATG+TGG + tig0025135:2184-2203 None:intergenic 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG + tig0025135:2379-2398 None:intergenic 25.0%
!! AAATAGCTTTGCATGTTTAA+TGG + tig0025135:2596-2615 None:intergenic 25.0%
! ATGTATTCTCAATGTATAGT+TGG + tig0025135:2777-2796 None:intergenic 25.0%
! GAGATTAAATTGCAATATAG+AGG - tig0025135:2801-2820 Msa1421520:intron 25.0%
! ATTTGGAAATACCAATAAGA+GGG - tig0025135:3129-3148 Msa1421520:CDS 25.0%
! CAGTATAAAAAGAATGATGT+TGG + tig0025135:3362-3381 None:intergenic 25.0%
!! TGTTCCTGACATTTTAAAGA+AGG + tig0025135:36-55 None:intergenic 30.0%
CAACAACAATTGCAAAAACA+AGG - tig0025135:54-73 Msa1421520:CDS 30.0%
TATGAGAATTGTTGTGACAT+AGG + tig0025135:204-223 None:intergenic 30.0%
! TTTTTGTTGAAGTGGATACA+TGG - tig0025135:675-694 Msa1421520:intron 30.0%
GAAGCAAATTAAGTGATCTT+AGG - tig0025135:766-785 Msa1421520:intron 30.0%
! GACTATCAGTTATTTCGATT+GGG + tig0025135:1248-1267 None:intergenic 30.0%
! TGACTATCAGTTATTTCGAT+TGG + tig0025135:1249-1268 None:intergenic 30.0%
TAATATAGTTCCAAAACTCC+AGG - tig0025135:1470-1489 Msa1421520:intron 30.0%
! TCAATCATAAAGAGGTTGAT+TGG + tig0025135:1510-1529 None:intergenic 30.0%
TTCTCTTCTCAATCATAAAG+AGG + tig0025135:1518-1537 None:intergenic 30.0%
! ACTTAAATCTGGAATTTGGA+TGG - tig0025135:1765-1784 Msa1421520:intron 30.0%
GTCTTGTGAAAATGCTTTAT+TGG + tig0025135:1820-1839 None:intergenic 30.0%
TGCAAATATACACAAACACT+TGG + tig0025135:1869-1888 None:intergenic 30.0%
CGAATTATTATGCTAAGTGA+TGG + tig0025135:1920-1939 None:intergenic 30.0%
ATATCATATCATTGAAGTGG+GGG + tig0025135:2338-2357 None:intergenic 30.0%
CATATCATATCATTGAAGTG+GGG + tig0025135:2339-2358 None:intergenic 30.0%
CCATATCATATCATTGAAGT+GGG + tig0025135:2340-2359 None:intergenic 30.0%
TCCATATCATATCATTGAAG+TGG + tig0025135:2341-2360 None:intergenic 30.0%
TTATTTATATTAGTGACCGG+AGG - tig0025135:2491-2510 Msa1421520:intron 30.0%
TATTTATATTAGTGACCGGA+GGG - tig0025135:2492-2511 Msa1421520:intron 30.0%
CAACTTGAATGCAATGATTT+AGG + tig0025135:2946-2965 None:intergenic 30.0%
GAGTTCGAAATTATTGTTTG+AGG + tig0025135:3077-3096 None:intergenic 30.0%
GATTTGGAAATACCAATAAG+AGG - tig0025135:3128-3147 Msa1421520:CDS 30.0%
! TTGTTTCATTTCTTGAGTTG+TGG + tig0025135:3297-3316 None:intergenic 30.0%
TTGAAATAGACAAGTCATCT+CGG + tig0025135:3397-3416 None:intergenic 30.0%
!! TTAAAGAAGGTGGTGGTTTT+AGG + tig0025135:23-42 None:intergenic 35.0%
!! TGACATTTTAAAGAAGGTGG+TGG + tig0025135:30-49 None:intergenic 35.0%
!! ACCACCTTCTTTAAAATGTC+AGG - tig0025135:29-48 Msa1421520:CDS 35.0%
!! TCCTGACATTTTAAAGAAGG+TGG + tig0025135:33-52 None:intergenic 35.0%
! TATAGGTTGAGGGCTATATA+GGG + tig0025135:153-172 None:intergenic 35.0%
! TTATAGGTTGAGGGCTATAT+AGG + tig0025135:154-173 None:intergenic 35.0%
ATATAGCCCTCAACCTATAA+AGG - tig0025135:154-173 Msa1421520:CDS 35.0%
TATAGCCCTCAACCTATAAA+GGG - tig0025135:155-174 Msa1421520:CDS 35.0%
! TGTAACCCTTTATAGGTTGA+GGG + tig0025135:163-182 None:intergenic 35.0%
ATGTAACCCTTTATAGGTTG+AGG + tig0025135:164-183 None:intergenic 35.0%
GTTGTGACATAGGAAATAGA+TGG + tig0025135:194-213 None:intergenic 35.0%
ATTCTCATAGTGTTGTCTGA+TGG - tig0025135:215-234 Msa1421520:CDS 35.0%
! AGGTTGTTGGCATGTTTTAT+TGG - tig0025135:259-278 Msa1421520:CDS 35.0%
!! TGTTTTATTGGCAACCATTC+AGG - tig0025135:271-290 Msa1421520:CDS 35.0%
TTGACGAAAAGGATTGCATA+TGG - tig0025135:416-435 Msa1421520:intron 35.0%
TATGTCAGCACAGTTATACA+TGG - tig0025135:455-474 Msa1421520:intron 35.0%
! TCAGTTATTTCGATTGGGTA+TGG + tig0025135:1243-1262 None:intergenic 35.0%
TGCATGAAGAGAAAGACATT+TGG - tig0025135:1885-1904 Msa1421520:intron 35.0%
CCCACTTCAATGATATGATA+TGG - tig0025135:2337-2356 Msa1421520:intron 35.0%
TGGAAAAAAAATACACCCTC+CGG - tig0025135:2357-2376 Msa1421520:intron 35.0%
!!! TTGCACATTGTCTTGTTTTC+AGG - tig0025135:2738-2757 Msa1421520:intron 35.0%
TTAGTTTATTCGCAACTGCT+AGG + tig0025135:2998-3017 None:intergenic 35.0%
AATCTTGCAGTTTCTCTTCT+GGG + tig0025135:3112-3131 None:intergenic 35.0%
AAATCTTGCAGTTTCTCTTC+TGG + tig0025135:3113-3132 None:intergenic 35.0%
AGAAGAGAAACTGCAAGATT+TGG - tig0025135:3112-3131 Msa1421520:CDS 35.0%
!!! TAGCTTGCTGGTCTAATTTT+TGG + tig0025135:3250-3269 None:intergenic 35.0%
!! GAACAAAACATTTAGCTTGC+TGG + tig0025135:3262-3281 None:intergenic 35.0%
! GATTTTTGTAGGCTATGGAT+GGG + tig0025135:3337-3356 None:intergenic 35.0%
! AGATTTTTGTAGGCTATGGA+TGG + tig0025135:3338-3357 None:intergenic 35.0%
! TGATGTTGGGAGATTTTTGT+AGG + tig0025135:3348-3367 None:intergenic 35.0%
AACATGCCAACAACCTCTAA+CGG + tig0025135:255-274 None:intergenic 40.0%
GTTATACGACCCGATAACTT+CGG - tig0025135:323-342 Msa1421520:CDS 40.0%
ATACAGTTGCCGAAGTTATC+GGG + tig0025135:335-354 None:intergenic 40.0%
CATACAGTTGCCGAAGTTAT+CGG + tig0025135:336-355 None:intergenic 40.0%
!! GTCAACAAGTGTGTCGATTT+GGG + tig0025135:401-420 None:intergenic 40.0%
GTTATACATGGTGCACAAGA+AGG - tig0025135:467-486 Msa1421520:intron 40.0%
ATGTAAGCAAACCGAGGATA+GGG + tig0025135:575-594 None:intergenic 40.0%
AATGTAAGCAAACCGAGGAT+AGG + tig0025135:576-595 None:intergenic 40.0%
TTGTTGAAGTGGATACATGG+AGG - tig0025135:678-697 Msa1421520:intron 40.0%
GCTCATAGTTACCAACATGT+AGG + tig0025135:1221-1240 None:intergenic 40.0%
CAAAACTCCAGGAATCGATT+GGG - tig0025135:1481-1500 Msa1421520:intron 40.0%
AAAACTCCAGGAATCGATTG+GGG - tig0025135:1482-1501 Msa1421520:intron 40.0%
GTCACTATGCATTTGTCACT+AGG - tig0025135:1575-1594 Msa1421520:intron 40.0%
GTTACGCAGACATATGAGTT+TGG + tig0025135:1798-1817 None:intergenic 40.0%
ACAAACACTTGGAGACAGAT+TGG + tig0025135:1858-1877 None:intergenic 40.0%
TGCTTATATTAGTGACCGGA+GGG + tig0025135:2375-2394 None:intergenic 40.0%
TTGCTTATATTAGTGACCGG+AGG + tig0025135:2376-2395 None:intergenic 40.0%
TAAGAATCAACTTCACCCTC+CGG + tig0025135:2510-2529 None:intergenic 40.0%
!!! CATTGTCTTGTTTTCAGGCA+AGG - tig0025135:2743-2762 Msa1421520:intron 40.0%
TGTGATACTATGGAGAAGCT+TGG + tig0025135:2899-2918 None:intergenic 40.0%
CCAGTGTAGTTGTGATACTA+TGG + tig0025135:2909-2928 None:intergenic 40.0%
CCATAGTATCACAACTACAC+TGG - tig0025135:2906-2925 Msa1421520:CDS 40.0%
GGAAATACCAATAAGAGGGA+TGG - tig0025135:3133-3152 Msa1421520:CDS 40.0%
!! TCCTGTTGGTGTTATTGATG+AGG - tig0025135:3184-3203 Msa1421520:CDS 40.0%
TCCTCATCAATAACACCAAC+AGG + tig0025135:3188-3207 None:intergenic 40.0%
GTTATTGATGAGGAGCTAAG+AGG - tig0025135:3194-3213 Msa1421520:CDS 40.0%
TGAGTTGTGGTTGCATCAAA+AGG + tig0025135:3284-3303 None:intergenic 40.0%
! TGGGAGATTTTTGTAGGCTA+TGG + tig0025135:3342-3361 None:intergenic 40.0%
! GAGTTGTTTCGAACTCGTCT+TGG - tig0025135:80-99 Msa1421520:CDS 45.0%
ACCTTAGCATGCAGCATAGT+TGG - tig0025135:119-138 Msa1421520:CDS 45.0%
ACCAACTATGCTGCATGCTA+AGG + tig0025135:123-142 None:intergenic 45.0%
GTGTTGTCTGATGGAACACT+TGG - tig0025135:224-243 Msa1421520:CDS 45.0%
CGATAACTTCGGCAACTGTA+TGG - tig0025135:334-353 Msa1421520:CDS 45.0%
!! CGTCAACAAGTGTGTCGATT+TGG + tig0025135:402-421 None:intergenic 45.0%
! CGACACACTTGTTGACGAAA+AGG - tig0025135:405-424 Msa1421520:intron 45.0%
GGCTACGCATTCAATTCATG+TGG - tig0025135:493-512 Msa1421520:intron 45.0%
TGGAGCTATAGCTTCTGTTC+TGG - tig0025135:532-551 Msa1421520:CDS 45.0%
! TCCTCGGTTTGCTTACATTG+AGG - tig0025135:577-596 Msa1421520:CDS 45.0%
ACCTCAATGTAAGCAAACCG+AGG + tig0025135:581-600 None:intergenic 45.0%
AAACGACTCTGCCTACATGT+TGG - tig0025135:1207-1226 Msa1421520:intron 45.0%
!! CCAATCGATTCCTGGAGTTT+TGG + tig0025135:1483-1502 None:intergenic 45.0%
CCAAAACTCCAGGAATCGAT+TGG - tig0025135:1480-1499 Msa1421520:intron 45.0%
GCAGACATATGAGTTTGGAC+TGG + tig0025135:1793-1812 None:intergenic 45.0%
! AACAGCAGTTGAGTTGTCTG+AGG + tig0025135:2836-2855 None:intergenic 45.0%
TCAGACAACTCAACTGCTGT+TGG - tig0025135:2835-2854 Msa1421520:intron 45.0%
ACAAACAGGATGGCATGCAT+TGG - tig0025135:3045-3064 Msa1421520:CDS 45.0%
CAAACAGGATGGCATGCATT+GGG - tig0025135:3046-3065 Msa1421520:CDS 45.0%
GCAATGTCCATCCCTCTTAT+TGG + tig0025135:3143-3162 None:intergenic 45.0%
ATGGACATTGCTCTATCGAG+TGG - tig0025135:3152-3171 Msa1421520:CDS 45.0%
! AGTGGCATGTCTTTTCCTGT+TGG - tig0025135:3170-3189 Msa1421520:CDS 45.0%
ACATGGTGCACAAGAAGGAG+TGG - tig0025135:472-491 Msa1421520:intron 50.0%
AGCTATAGCTCCAAGTGCAG+TGG + tig0025135:525-544 None:intergenic 50.0%
! TTGGATCCCCAATCGATTCC+TGG + tig0025135:1491-1510 None:intergenic 50.0%
! CATTGAAGTGGGGGTTAGTG+AGG + tig0025135:2329-2348 None:intergenic 50.0%
! TGAGTTGTCTGAGGCTGAGA+TGG + tig0025135:2827-2846 None:intergenic 50.0%
!! TCCTGTTGTGGAGAGGTACT+TGG + tig0025135:2875-2894 None:intergenic 50.0%
!! ACACTTGGTGACGCCGTTAG+AGG - tig0025135:239-258 Msa1421520:CDS 55.0%
GTGACGCCGTTAGAGGTTGT+TGG - tig0025135:246-265 Msa1421520:CDS 55.0%
AGTGGCTACCGGTGCATTTG+TGG - tig0025135:367-386 Msa1421520:intron 55.0%
!! GTGGCTTGTTCCACTGCACT+TGG - tig0025135:512-531 Msa1421520:CDS 55.0%
AGCAAACCGAGGATAGGGCA+GGG + tig0025135:570-589 None:intergenic 55.0%
AAGCAAACCGAGGATAGGGC+AGG + tig0025135:571-590 None:intergenic 55.0%
GCCAAGTACCTCTCCACAAC+AGG - tig0025135:2871-2890 Msa1421520:CDS 55.0%
GAGAAGCTTGGCTCCTGTTG+TGG + tig0025135:2887-2906 None:intergenic 55.0%
GGCTACCGGTGCATTTGTGG+TGG - tig0025135:370-389 Msa1421520:intron 60.0%
CAGCGCCACCACAAATGCAC+CGG + tig0025135:378-397 None:intergenic 60.0%
ATAGGGCAGGGGCAGCAACA+TGG + tig0025135:558-577 None:intergenic 60.0%
GCAAACCGAGGATAGGGCAG+GGG + tig0025135:569-588 None:intergenic 60.0%
GCTTGGCTCCTGTTGTGGAG+AGG + tig0025135:2882-2901 None:intergenic 60.0%
CGGCAACTGTATGGCTGCGC+TGG - tig0025135:343-362 Msa1421520:CDS 65.0%
! CTGTATGGCTGCGCTGGCAG+TGG - tig0025135:349-368 Msa1421520:CDS 65.0%
TGCTGCCCCTGCCCTATCCT+CGG - tig0025135:561-580 Msa1421520:CDS 65.0%
! GCTGCGCTGGCAGTGGCTAC+CGG - tig0025135:356-375 Msa1421520:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0025135 gene 8 3427 8 ID=Msa1421520;Name=Msa1421520
tig0025135 mRNA 8 3427 8 ID=Msa1421520-mRNA-1;Parent=Msa1421520;Name=Msa1421520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=36|1|0.66|1|1|1|3|0|366
tig0025135 exon 8 382 8 ID=Msa1421520-mRNA-1:exon:2171;Parent=Msa1421520-mRNA-1
tig0025135 exon 508 675 508 ID=Msa1421520-mRNA-1:exon:2170;Parent=Msa1421520-mRNA-1
tig0025135 exon 2837 3427 2837 ID=Msa1421520-mRNA-1:exon:2169;Parent=Msa1421520-mRNA-1
tig0025135 five_prime_UTR 3392 3427 3392 ID=Msa1421520-mRNA-1:five_prime_utr;Parent=Msa1421520-mRNA-1
tig0025135 CDS 2837 3391 2837 ID=Msa1421520-mRNA-1:cds;Parent=Msa1421520-mRNA-1
tig0025135 CDS 508 675 508 ID=Msa1421520-mRNA-1:cds;Parent=Msa1421520-mRNA-1
tig0025135 CDS 8 382 8 ID=Msa1421520-mRNA-1:cds;Parent=Msa1421520-mRNA-1
Gene Sequence

>Msa1421520

ATGTCAGGAACAACAACAATTGCAAAAACAAGGACAGAGTTGTTTCGAACTCGTCTTGGTTCTGCTTTAAGAACAACCTTAGCATGCAGCATAGTTGGTTGCACTGCCCTATATAGCCCTCAACCTATAAAGGGTTACATTAAATTTCCATCTATTTCCTATGTCACAACAATTCTCATAGTGTTGTCTGATGGAACACTTGGTGACGCCGTTAGAGGTTGTTGGCATGTTTTATTGGCAACCATTCAGGTTATGATTTTTTCTCTACTTAGCTTGCAAGTTATACGACCCGATAACTTCGGCAACTGTATGGCTGCGCTGGCAGTGGCTACCGGTGCATTTGTGGTGGCGCTGCCCAAATCGACACACTTGTTGACGAAAAGGATTGCATATGGACAGCTTGTGATAGTATATGTCAGCACAGTTATACATGGTGCACAAGAAGGAGTGGCTACGCATTCAATTCATGTGGCTTGTTCCACTGCACTTGGAGCTATAGCTTCTGTTCTGGCCATGTTGCTGCCCCTGCCCTATCCTCGGTTTGCTTACATTGAGGCAAGGAAGTTCAACCAACTATACATTGAGAATACATCTGAGAGATTAAATTGCAATATAGAGGCCATCTCAGCCTCAGACAACTCAACTGCTGTTGGTTTTATAACTAAAGCCAAGTACCTCTCCACAACAGGAGCCAAGCTTCTCCATAGTATCACAACTACACTGGATGGCATGCATTGGGAGAGACCTCAAACAATAATTTCGAACTCTTGTTGCATTGACCCAGAAGAGAAACTGCAAGATTTGGAAATACCAATAAGAGGGATGGACATTGCTCTATCGAGTGGCATGTCTTTTCCTGTTGGTGTTATTGATGAGGAGCTAAGAGGTGTTCTGCTCAATTGCAGAGAACAAATCAGCCAAAAATTAGACCAGCAAGCTAAATGTTTTGTTCCTTTTGATGCAACCACAACTCAAGAAATGAAACAAGACATTTTCAACAAAAACCCATCCATAGCCTACAAAAATCTCCCAACATCATTCTTTTTATACTGTGTGCAACTTCTCCGAGATGACTTGTCTATTTCAAAGAAAACTGAC
Protein sequence

>Msa1421520

MSGTTTIAKTRTELFRTRLGSALRTTLACSIVGCTALYSPQPIKGYIKFPSISYVTTILIVLSDGTLGDAVRGCWHVLLATIQVMIFSLLSLQVIRPDNFGNCMAALAVATGAFVVALPKSTHLLTKRIAYGQLVIVYVSTVIHGAQEGVATHSIHVACSTALGAIASVLAMLLPLPYPRFAYIEARKFNQLYIENTSERLNCNIEAISASDNSTAVGFITKAKYLSTTGAKLLHSITTTLDGMHWERPQTIISNSCCIDPEEKLQDLEIPIRGMDIALSSGMSFPVGVIDEELRGVLLNCREQISQKLDQQAKCFVPFDATTTQEMKQDIFNKNPSIAYKNLPTSFFLYCVQLLRDDLSISKKTD