AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0555890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555890 MtrunA17_Chr4g0042111 94.840 562 29 0 1 562 1 562 0.0 1122
Msa0555890 MtrunA17_Chr7g0236991 56.273 542 234 2 22 562 14 553 0.0 652
Msa0555890 MtrunA17_Chr7g0237001 56.273 542 234 2 22 562 14 553 0.0 651
Msa0555890 MtrunA17_Chr7g0237041 56.273 542 234 2 22 562 14 553 0.0 651
Msa0555890 MtrunA17_Chr7g0237081 56.273 542 234 2 22 562 14 553 0.0 651
Msa0555890 MtrunA17_Chr7g0237051 56.273 542 234 2 22 562 14 553 0.0 651
Msa0555890 MtrunA17_Chr3g0105071 55.914 558 242 3 7 562 2 557 0.0 647
Msa0555890 MtrunA17_Chr5g0425371 55.556 549 240 3 16 562 7 553 0.0 647
Msa0555890 MtrunA17_Chr4g0024691 56.727 550 235 2 14 562 14 561 0.0 645
Msa0555890 MtrunA17_Chr6g0451711 54.281 549 249 2 15 562 10 557 0.0 639
Msa0555890 MtrunA17_Chr5g0431571 55.941 547 237 3 18 562 5 549 0.0 639
Msa0555890 MtrunA17_Chr3g0082691 54.595 555 248 3 10 562 1 553 0.0 637
Msa0555890 MtrunA17_Chr6g0465911 54.399 557 248 3 7 562 2 553 0.0 635
Msa0555890 MtrunA17_Chr6g0466051 54.219 557 249 3 7 562 2 553 0.0 634
Msa0555890 MtrunA17_Chr5g0431651 54.098 549 249 3 16 562 5 552 0.0 628
Msa0555890 MtrunA17_Chr6g0465441 52.727 550 256 3 15 562 6 553 0.0 624
Msa0555890 MtrunA17_Chr7g0236961 57.551 490 208 0 73 562 9 498 0.0 611
Msa0555890 MtrunA17_Chr7g0237011 57.551 490 208 0 73 562 9 498 0.0 610
Msa0555890 MtrunA17_Chr6g0465851 54.808 520 232 2 44 562 1 518 0.0 602
Msa0555890 MtrunA17_Chr2g0319041 45.730 562 291 10 8 562 3 557 3.04e-176 509
Msa0555890 MtrunA17_Chr7g0236971 57.357 401 171 0 162 562 1 401 2.35e-174 498
Msa0555890 MtrunA17_Chr8g0336181 46.558 552 284 8 18 562 9 556 1.02e-172 500
Msa0555890 MtrunA17_Chr2g0319051 45.946 518 272 7 49 562 1 514 3.57e-165 479
Msa0555890 MtrunA17_Chr6g0465981 56.878 378 157 3 7 383 2 374 5.45e-154 446
Msa0555890 MtrunA17_Chr6g0465871 56.878 378 157 3 7 383 2 374 5.45e-154 446
Msa0555890 MtrunA17_Chr6g0466081 56.878 378 157 3 7 383 2 374 5.45e-154 446
Msa0555890 MtrunA17_Chr6g0466111 57.771 341 141 2 44 383 1 339 4.07e-141 411
Msa0555890 MtrunA17_Chr7g0236951 60.125 321 126 1 63 383 1 319 7.29e-141 410
Msa0555890 MtrunA17_Chr7g0237031 60.125 321 126 1 63 383 1 319 1.14e-140 409
Msa0555890 MtrunA17_Chr6g0465931 57.478 341 142 2 44 383 1 339 5.71e-140 409
Msa0555890 MtrunA17_Chr4g0008621 46.778 419 211 6 8 416 2 418 8.55e-135 399
Msa0555890 MtrunA17_Chr4g0023911 37.906 554 337 5 7 559 31 578 1.23e-124 378
Msa0555890 MtrunA17_Chr2g0310331 38.321 548 323 9 18 561 49 585 1.11e-116 358
Msa0555890 MtrunA17_Chr2g0319071 38.791 513 262 10 51 559 22 486 1.57e-114 349
Msa0555890 MtrunA17_Chr8g0357411 36.182 550 341 5 15 562 48 589 7.56e-112 345
Msa0555890 MtrunA17_Chr2g0323131 36.454 502 304 6 41 539 55 544 6.25e-109 337
Msa0555890 MtrunA17_Chr2g0323151 37.931 464 275 5 79 539 6 459 6.32e-105 324
Msa0555890 MtrunA17_Chr5g0445511 61.233 227 88 0 158 384 2 228 3.64e-101 305
Msa0555890 MtrunA17_Chr6g0466031 50.000 260 130 0 303 562 1 260 6.06e-91 280
Msa0555890 MtrunA17_Chr4g0048911 34.151 489 308 9 58 539 60 541 1.81e-89 286
Msa0555890 MtrunA17_Chr2g0310401 37.150 428 266 2 136 561 5 431 2.87e-89 281
Msa0555890 MtrunA17_Chr4g0048921 35.579 475 299 4 72 542 82 553 9.41e-89 285
Msa0555890 MtrunA17_Chr7g0237061 48.846 260 110 2 303 562 1 237 3.65e-82 256
Msa0555890 MtrunA17_Chr7g0237071 60.309 194 77 0 73 266 9 202 1.62e-77 244
Msa0555890 MtrunA17_Chr2g0280581 29.480 519 350 9 50 558 6 518 3.98e-77 253
Msa0555890 MtrunA17_Chr2g0319081 38.393 336 187 8 230 562 109 427 2.52e-73 240
Msa0555890 MtrunA17_Chr2g0319081 40.580 138 77 4 1 136 1 135 2.38e-21 97.1
Msa0555890 MtrunA17_Chr3g0108941 29.435 496 333 8 50 535 6 494 8.59e-73 241
Msa0555890 MtrunA17_Chr3g0108981 30.407 467 312 7 79 535 6 469 2.45e-72 239
Msa0555890 MtrunA17_Chr3g0108961 30.407 467 312 7 79 535 6 469 2.45e-72 239
Msa0555890 MtrunA17_Chr3g0109001 30.040 496 330 9 50 535 6 494 6.11e-72 239
Msa0555890 MtrunA17_Chr3g0105691 29.651 516 341 11 50 552 39 545 5.20e-71 237
Msa0555890 MtrunA17_Chr3g0109021 29.659 499 327 11 50 535 6 493 6.18e-70 234
Msa0555890 MtrunA17_Chr3g0109031 29.234 496 333 10 50 535 6 493 2.10e-69 232
Msa0555890 MtrunA17_Chr3g0109011 30.043 466 314 7 79 535 6 468 4.32e-69 231
Msa0555890 MtrunA17_Chr3g0099031 30.382 497 326 11 50 535 6 493 6.64e-69 231
Msa0555890 MtrunA17_Chr3g0108991 29.234 496 333 10 50 535 6 493 3.32e-68 229
Msa0555890 MtrunA17_Chr3g0108951 29.234 496 333 10 50 535 6 493 3.32e-68 229
Msa0555890 MtrunA17_Chr6g0466021 53.171 205 90 3 7 210 2 201 7.95e-68 218
Msa0555890 MtrunA17_Chr3g0108971 30.407 467 311 9 79 535 6 468 2.31e-67 226
Msa0555890 MtrunA17_Chr6g0477111 29.072 485 330 7 79 552 6 487 7.20e-67 225
Msa0555890 MtrunA17_Chr7g0237021 51.163 172 84 0 391 562 1 172 4.15e-59 194
Msa0555890 MtrunA17_Chr7g0236941 51.163 172 84 0 391 562 1 172 4.15e-59 194
Msa0555890 MtrunA17_Chr6g0477121 29.529 403 277 5 139 535 10 411 4.55e-58 199
Msa0555890 MtrunA17_Chr5g0445501 49.419 172 87 0 391 562 1 172 8.19e-58 191
Msa0555890 MtrunA17_Chr7g0217361 36.000 300 191 1 261 559 8 307 1.54e-56 192
Msa0555890 MtrunA17_Chr6g0465881 48.256 172 89 0 391 562 1 172 1.01e-54 182
Msa0555890 MtrunA17_Chr6g0465941 48.256 172 89 0 391 562 1 172 1.17e-54 182
Msa0555890 MtrunA17_Chr6g0466121 48.256 172 89 0 391 562 1 172 1.17e-54 182
Msa0555890 MtrunA17_Chr6g0465991 48.256 172 89 0 391 562 1 172 1.17e-54 182
Msa0555890 MtrunA17_Chr6g0466091 48.256 172 89 0 391 562 1 172 1.17e-54 182
Msa0555890 MtrunA17_Chr8g0357231 34.363 259 168 2 304 561 1 258 1.28e-46 164
Msa0555890 MtrunA17_Chr7g0236921 48.101 158 78 1 409 562 1 158 5.65e-46 159
Msa0555890 MtrunA17_Chr8g0357221 38.400 250 146 3 15 263 48 290 1.76e-43 156
Msa0555890 MtrunA17_Chr7g0236931 46.104 154 80 1 409 562 1 151 3.66e-42 148
Msa0555890 MtrunA17_Chr7g0233861 52.308 130 53 1 207 336 38 158 1.58e-41 147
Msa0555890 MtrunA17_Chr2g0310121 28.150 508 322 17 79 553 278 775 9.90e-39 151
Msa0555890 MtrunA17_Chr3g0101681 28.544 522 314 20 79 558 279 783 2.56e-37 147
Msa0555890 MtrunA17_Chr7g0236981 48.760 121 59 2 22 141 14 132 2.32e-33 124
Msa0555890 MtrunA17_Chr5g0445401 59.140 93 36 2 173 263 2 94 7.22e-28 107
Msa0555890 MtrunA17_Chr7g0218001 28.617 311 177 8 65 348 335 627 6.78e-23 103
Msa0555890 MtrunA17_Chr5g0445521 48.980 98 47 2 7 103 2 97 1.62e-22 92.4
Msa0555890 MtrunA17_Chr3g0082711 40.517 116 52 1 447 562 1 99 3.27e-22 91.7
Msa0555890 MtrunA17_Chr7g0217351 30.412 194 129 3 74 264 83 273 1.60e-21 95.1
Msa0555890 MtrunA17_Chr7g0259881 26.241 282 180 7 65 319 218 498 1.77e-21 98.6
Msa0555890 MtrunA17_Chr2g0281651 24.825 286 204 7 239 519 43 322 3.74e-17 84.0
Msa0555890 MtrunA17_Chr2g0310411 41.379 116 59 5 21 136 57 163 1.03e-16 78.2
Msa0555890 MtrunA17_Chr7g0218041 30.705 241 142 6 124 340 1 240 4.39e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555890 AT5G23960.2 42.701 548 308 4 17 562 2 545 1.33e-156 459
Msa0555890 AT5G23960.1 42.727 550 307 5 17 562 2 547 2.81e-155 456
Msa0555890 AT3G14490.1 40.143 558 323 6 11 562 49 601 1.04e-140 420
Msa0555890 AT1G70080.1 37.590 556 337 7 11 558 54 607 8.92e-130 392
Msa0555890 AT3G14540.1 36.563 547 338 4 21 562 59 601 4.10e-127 385
Msa0555890 AT3G14520.1 36.197 547 340 4 21 562 62 604 4.34e-127 385
Msa0555890 AT1G31950.1 35.846 544 340 4 24 562 67 606 1.64e-125 381
Msa0555890 AT1G31950.3 35.780 545 340 5 24 562 67 607 2.98e-124 378
Msa0555890 AT3G32030.1 37.011 562 338 8 11 562 49 604 4.49e-123 375
Msa0555890 AT5G44630.1 37.640 534 325 6 32 559 23 554 1.81e-119 364
Msa0555890 AT1G33750.1 34.813 563 360 4 2 562 46 603 8.38e-119 364
Msa0555890 AT3G29410.1 35.531 546 343 6 21 562 63 603 2.20e-118 363
Msa0555890 AT3G29410.2 35.259 502 318 4 63 562 2 498 8.29e-111 340
Msa0555890 AT4G20200.1 36.015 522 328 4 45 562 85 604 4.60e-108 336
Msa0555890 AT4G15870.1 33.993 556 345 8 10 558 54 594 1.45e-107 335
Msa0555890 AT4G16730.1 33.270 529 331 7 48 562 19 539 3.53e-107 332
Msa0555890 AT4G20210.1 31.915 564 374 5 3 562 43 600 2.78e-106 332
Msa0555890 AT4G13300.1 35.382 537 331 6 32 559 22 551 7.64e-105 326
Msa0555890 AT4G16730.2 33.333 495 317 4 79 562 1 493 1.65e-104 323
Msa0555890 AT3G14540.2 36.865 453 278 3 21 469 59 507 1.25e-102 319
Msa0555890 AT4G20230.2 34.023 532 344 6 32 558 84 613 8.77e-102 320
Msa0555890 AT4G20230.1 34.023 532 344 6 32 558 72 601 1.01e-101 320
Msa0555890 AT3G29190.1 33.581 539 344 6 32 562 69 601 1.29e-101 319
Msa0555890 AT4G13280.2 35.460 533 336 6 32 559 22 551 1.50e-101 318
Msa0555890 AT2G23230.2 34.307 548 351 8 6 546 39 584 5.44e-101 318
Msa0555890 AT2G23230.1 34.124 548 347 9 6 546 39 579 7.34e-99 312
Msa0555890 AT1G48800.1 33.092 553 361 6 12 558 53 602 1.05e-98 312
Msa0555890 AT1G31950.2 36.018 447 277 4 24 465 67 509 1.27e-98 309
Msa0555890 AT1G66020.1 33.710 531 346 4 32 558 66 594 5.14e-97 307
Msa0555890 AT4G16740.1 32.671 554 347 11 21 562 25 564 3.01e-96 304
Msa0555890 AT3G25820.1 31.325 581 361 12 5 562 29 594 4.13e-96 305
Msa0555890 AT3G25830.1 31.325 581 361 12 5 562 29 594 4.13e-96 305
Msa0555890 AT3G29110.1 32.772 534 353 5 29 558 62 593 1.31e-95 303
Msa0555890 AT2G24210.1 32.635 573 345 13 13 562 36 590 1.74e-95 303
Msa0555890 AT1G61680.1 33.811 488 309 6 58 539 77 556 2.26e-93 297
Msa0555890 AT1G61680.2 33.607 488 303 7 58 539 77 549 2.24e-91 291
Msa0555890 AT3G25810.1 32.095 592 345 16 5 562 29 597 3.05e-90 290
Msa0555890 AT4G13280.1 33.959 533 314 7 32 559 22 521 5.98e-90 286
Msa0555890 AT1G48820.1 32.240 549 311 11 21 558 62 560 4.89e-81 265
Msa0555890 AT5G48110.1 38.484 343 206 5 221 562 1 339 1.63e-78 251
Msa0555890 AT3G25820.2 33.741 409 246 7 5 399 29 426 1.12e-71 236
Msa0555890 AT4G16740.2 35.544 377 224 7 21 387 25 392 5.61e-70 231
Msa0555890 AT1G66020.2 36.103 349 217 4 32 376 66 412 1.21e-67 225
Msa0555890 AT3G25820.4 35.838 346 197 7 5 336 29 363 1.43e-61 208
Msa0555890 AT3G25820.3 35.838 346 197 7 5 336 29 363 1.43e-61 208
Msa0555890 AT4G16740.4 37.730 326 184 7 21 336 25 341 4.85e-61 206
Msa0555890 AT4G16740.3 37.730 326 184 7 21 336 25 341 4.85e-61 206
Msa0555890 AT1G79460.1 24.606 508 340 16 78 556 276 769 1.69e-29 124
Msa0555890 AT1G61120.1 22.649 521 365 14 65 559 273 781 8.34e-26 112
Msa0555890 AT4G02780.1 30.824 279 161 8 65 314 320 595 2.68e-24 108

Find 109 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAATTGAAATCTAATT+TGG 0.157846 4_1:+53904314 None:intergenic
AGATGAAGCATTGACAATTT+TGG 0.158357 4_1:+53903348 None:intergenic
GATGATATATATGATGTTTA+TGG 0.206526 4_1:-53903994 Msa0555890:CDS
CACAATAATCATGTTGAAAA+TGG 0.211729 4_1:-53905158 Msa0555890:CDS
GAGAAATCGGAGTTGGTATT+AGG 0.224064 4_1:+53906174 None:intergenic
ATTCAATTGCACTTGTCTTT+AGG 0.255161 4_1:-53905109 Msa0555890:CDS
CAAAGTCCCTTTACTCATAT+TGG 0.261866 4_1:+53902909 None:intergenic
CAAATTACCACCCCAACATT+TGG 0.286981 4_1:-53906140 Msa0555890:CDS
AAATTACCACCCCAACATTT+GGG 0.288674 4_1:-53906139 Msa0555890:CDS
CCAAGACATATTGAATAAAA+AGG 0.295172 4_1:+53906112 None:intergenic
GTCATTCTTTGTTGATCCTT+GGG 0.305154 4_1:+53902959 None:intergenic
AGAGGCTTTGGCACTTGAAT+TGG 0.307687 4_1:+53903053 None:intergenic
GATTGATTCAATCCAACGTT+TGG 0.313958 4_1:-53905231 Msa0555890:CDS
GACAAACTAGCCATTGAAAT+AGG 0.327953 4_1:-53902804 Msa0555890:three_prime_UTR
AGAAATCGGAGTTGGTATTA+GGG 0.328348 4_1:+53906175 None:intergenic
TGTCATTCTTTGTTGATCCT+TGG 0.335528 4_1:+53902958 None:intergenic
GAATAGTTGAAGCTTACTTT+TGG 0.351708 4_1:-53904098 Msa0555890:CDS
GCTCTTGGAATAGTAAATTC+TGG 0.365262 4_1:-53903454 Msa0555890:CDS
TTTGTGCTTGATTACCTCAT+TGG 0.372285 4_1:+53903294 None:intergenic
CACTTGTCTTTAGGTTACTA+AGG 0.384876 4_1:-53905100 Msa0555890:CDS
TCTTCTTATGCCTATTTCAA+TGG 0.389051 4_1:+53902794 None:intergenic
GCCATGTACTCTTCTATTGT+TGG 0.400220 4_1:+53903475 None:intergenic
TTTGGATGTTTATGAAGAAA+TGG 0.403778 4_1:-53903775 Msa0555890:CDS
ATTGATTCAATCCAACGTTT+GGG 0.408878 4_1:-53905230 Msa0555890:CDS
CTCAATACTCTGTTGGTAGA+AGG 0.414840 4_1:-53904053 Msa0555890:CDS
CAAGAACTTGCCTAGGCTAC+TGG 0.415662 4_1:-53904400 Msa0555890:CDS
GCATTACATTTCCACTTATG+AGG 0.423418 4_1:-53904373 Msa0555890:CDS
TCCATGTCCAATATGAGTAA+AGG 0.424065 4_1:-53902916 Msa0555890:exon
TCAATATGTCTTGGAACCTA+TGG 0.434054 4_1:-53906103 Msa0555890:intron
GCCACACATATGAGGATTCA+TGG 0.435188 4_1:-53904540 Msa0555890:CDS
TCAAATTAGCTTCCCTCAAA+GGG 0.439126 4_1:+53905252 None:intergenic
AACAACATCCTTCGTTGGAA+TGG 0.440069 4_1:-53903416 Msa0555890:CDS
CTCCGATTTCTCTAGACGTT+CGG 0.441215 4_1:-53906163 Msa0555890:CDS
CCATGTCCAATATGAGTAAA+GGG 0.444601 4_1:-53902915 Msa0555890:exon
CCCTTTACTCATATTGGACA+TGG 0.445169 4_1:+53902915 None:intergenic
TTAATTTCTGTAGCAAGAAA+AGG 0.447664 4_1:+53904447 None:intergenic
TGCCGAACGTCTAGAGAAAT+CGG 0.450185 4_1:+53906161 None:intergenic
ATAGAAGAGTACATGGCTCT+TGG 0.456178 4_1:-53903469 Msa0555890:CDS
ACTATTCTGTTGCGAGCAAA+AGG 0.456530 4_1:+53904114 None:intergenic
ACAACATCCTTCGTTGGAAT+GGG 0.463666 4_1:-53903415 Msa0555890:CDS
ATTGATGTGCTTTATAAAGA+TGG 0.464933 4_1:-53902999 Msa0555890:CDS
CATACTTTACTGAGGCTCAA+TGG 0.470064 4_1:-53903516 Msa0555890:CDS
AAAATGTCACAAACCCTTTG+AGG 0.477504 4_1:-53905265 Msa0555890:CDS
AAACAACATCAGAAAGAAGT+TGG 0.478105 4_1:-53904288 Msa0555890:CDS
TGTATGAAGCCACACATATG+AGG 0.482529 4_1:-53904548 Msa0555890:CDS
ATCAAATTAGCTTCCCTCAA+AGG 0.485801 4_1:+53905251 None:intergenic
ACTCGCATAAGGAGAGGCTT+TGG 0.486941 4_1:+53903041 None:intergenic
ATCTAAAATTGCTAGACTCA+TGG 0.490115 4_1:-53903329 Msa0555890:CDS
GAGCCTCAGTAAAGTATGCC+TGG 0.491242 4_1:+53903521 None:intergenic
CAAGACAAGTCACAAATGCT+TGG 0.491285 4_1:-53903103 Msa0555890:CDS
TGCACAATAGATGAAAAGAT+TGG 0.497980 4_1:-53903545 Msa0555890:intron
CAATGTCATGCTCAAAATGA+TGG 0.500528 4_1:+53905201 None:intergenic
AACTTCAAAGAAACACTTGT+TGG 0.503713 4_1:-53904594 Msa0555890:CDS
TTCTTCATAAACATCCAAAA+CGG 0.505524 4_1:+53903779 None:intergenic
AGAGGGATCTTCCTCATAAG+TGG 0.512911 4_1:+53904362 None:intergenic
TAATGCCTTGCCAGTAGCCT+AGG 0.520942 4_1:+53904390 None:intergenic
AAATGTCACAAACCCTTTGA+GGG 0.523712 4_1:-53905264 Msa0555890:CDS
ATTGAGTGGTCATCATTTCA+AGG 0.523890 4_1:+53904475 None:intergenic
TCGGAGTTGGTATTAGGGGT+TGG 0.524425 4_1:+53906180 None:intergenic
GAAATCGGAGTTGGTATTAG+GGG 0.527233 4_1:+53906176 None:intergenic
TTTGAGCCTCAATACTCTGT+TGG 0.527980 4_1:-53904060 Msa0555890:CDS
TAGCAAAATTTCATGTATTG+TGG 0.530446 4_1:+53903807 None:intergenic
ATCATCAATTACTGAGGCCA+AGG 0.530914 4_1:+53904011 None:intergenic
ACAAATTGAGAACTCGCATA+AGG 0.532358 4_1:+53903030 None:intergenic
AAGAAGTTCAAGAATGAGCA+AGG 0.540053 4_1:-53904618 Msa0555890:CDS
ATCACAACAACATCCTTCGT+TGG 0.542322 4_1:-53903421 Msa0555890:CDS
ATAGATGAAAAGATTGGTCC+AGG 0.544707 4_1:-53903539 Msa0555890:intron
ACAAGTCACAAATGCTTGGA+AGG 0.546592 4_1:-53903099 Msa0555890:CDS
AATGACCAAGGTGATATCCT+TGG 0.547258 4_1:-53904028 Msa0555890:CDS
TTTGAGCAAGAGAGAGGGCA+TGG 0.551451 4_1:-53903194 Msa0555890:CDS
AGACAAGTGCAATTGAATGA+AGG 0.552478 4_1:+53905114 None:intergenic
TAGGTGGGATATAAGTTGCA+TGG 0.561499 4_1:-53903838 Msa0555890:CDS
AGCAAAGTTGCATCATGAGA+GGG 0.572891 4_1:+53904345 None:intergenic
AATTAGTTTGAGCAAGAGAG+AGG 0.573171 4_1:-53903200 Msa0555890:intron
GGATGATATTGTTTCCAATG+AGG 0.573519 4_1:-53903308 Msa0555890:intron
AACACTTGTTGGTGATGTTG+AGG 0.576884 4_1:-53904583 Msa0555890:CDS
ACACTTGTTGGTGATGTTGA+GGG 0.577847 4_1:-53904582 Msa0555890:CDS
TGAAGAAATGGAGCAAGAGA+TGG 0.580004 4_1:-53903763 Msa0555890:CDS
CACTCTTCAAGAACTTGCCT+AGG 0.581377 4_1:-53904407 Msa0555890:CDS
ATTACCACCCCAACATTTGG+GGG 0.583067 4_1:-53906137 Msa0555890:CDS
AATTACCACCCCAACATTTG+GGG 0.585116 4_1:-53906138 Msa0555890:CDS
ACCAACAATAGAAGAGTACA+TGG 0.590550 4_1:-53903476 Msa0555890:CDS
TCCATGAATCCTCATATGTG+TGG 0.591257 4_1:+53904539 None:intergenic
ATTAGTTTGAGCAAGAGAGA+GGG 0.599571 4_1:-53903199 Msa0555890:intron
CTTCTACCAACAGAGTATTG+AGG 0.601314 4_1:+53904054 None:intergenic
TAGCAAAGTTGCATCATGAG+AGG 0.612571 4_1:+53904344 None:intergenic
GGGATGCATTGCTACAGAAG+AGG 0.614970 4_1:-53903395 Msa0555890:CDS
GAGCAAGAGATGGTCAAGGA+AGG 0.615004 4_1:-53903753 Msa0555890:CDS
CTAGGCAAGTTCTTGAAGAG+TGG 0.622912 4_1:+53904408 None:intergenic
ACGTCTAGAGAAATCGGAGT+TGG 0.629425 4_1:+53906167 None:intergenic
AATGCATCCCATTCCAACGA+AGG 0.632549 4_1:+53903408 None:intergenic
GATGGTATAATCCCAAACGT+TGG 0.633632 4_1:+53905219 None:intergenic
ACATATGAGGATTCATGGAG+AGG 0.637251 4_1:-53904535 Msa0555890:CDS
TTACCACCCCAACATTTGGG+GGG 0.640939 4_1:-53906136 Msa0555890:CDS
TTTAGGTTACTAAGGCAACA+AGG 0.641189 4_1:-53905092 Msa0555890:CDS
AAATTAATCATAGCTTAAAG+CGG 0.641455 4_1:-53904431 Msa0555890:CDS
GGTCATCATTTCAAGGTGTG+AGG 0.643197 4_1:+53904482 None:intergenic
TGAGGCCAAGGATATCACCT+TGG 0.643385 4_1:+53904023 None:intergenic
GATTGCTATACTAACTCCCA+AGG 0.653661 4_1:-53902975 Msa0555890:CDS
AAGAAAAGGACTCAATTGAG+TGG 0.658799 4_1:+53904461 None:intergenic
AATGGAGCAAGAGATGGTCA+AGG 0.660378 4_1:-53903757 Msa0555890:CDS
TTGAGAACTCGCATAAGGAG+AGG 0.660485 4_1:+53903035 None:intergenic
ACTTGCCTAGGCTACTGGCA+AGG 0.666735 4_1:-53904395 Msa0555890:CDS
CATGTAGCACTCAATAGCAG+AGG 0.671786 4_1:+53903169 None:intergenic
ATATATATCATCAATTACTG+AGG 0.671981 4_1:+53904005 None:intergenic
GGTCCAGGCATACTTTACTG+AGG 0.678982 4_1:-53903524 Msa0555890:CDS
TGCTTGGAAGGATATCAATG+AGG 0.688978 4_1:-53903087 Msa0555890:CDS
TGGTAGAAGGATAATGACCA+AGG 0.706697 4_1:-53904040 Msa0555890:CDS
GAGTGCTACATGAATGAACA+TGG 0.715393 4_1:-53903158 Msa0555890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAAAATCACATAATT+TGG + chr4_1:53904157-53904176 None:intergenic 10.0%
!!! TGATTTTTTAAAATTATTAG+TGG - chr4_1:53904164-53904183 Msa0555890:intron 10.0%
!! ACAAAATTAAAATGATTAAA+TGG - chr4_1:53904299-53904318 Msa0555890:CDS 10.0%
!!! AATATTGAACTATTTTTTTA+TGG - chr4_1:53905095-53905114 Msa0555890:CDS 10.0%
!! TTTAATTCAAATTAATTAAG+AGG + chr4_1:53905132-53905151 None:intergenic 10.0%
!!! TTAATTTGAATTAAAATTCT+AGG - chr4_1:53905137-53905156 Msa0555890:CDS 10.0%
!!! AAATTTTACTCTTACTTTAA+TGG - chr4_1:53903082-53903101 Msa0555890:CDS 15.0%
!!! AATTTTACTCTTACTTTAAT+GGG - chr4_1:53903083-53903102 Msa0555890:CDS 15.0%
!! AATGCTATTAAAATTAAGTA+GGG + chr4_1:53903570-53903589 None:intergenic 15.0%
!! AAATGCTATTAAAATTAAGT+AGG + chr4_1:53903569-53903588 None:intergenic 15.0%
!!! AATTTTGCAATAATTTTTTG+CGG + chr4_1:53903314-53903333 None:intergenic 15.0%
!! AGATAAAAAGTAATAAAATC+TGG + chr4_1:53903349-53903368 None:intergenic 15.0%
!! TTCAAAAATTTAAGAGTTTA+AGG - chr4_1:53903480-53903499 Msa0555890:CDS 15.0%
!! AAATGCTATTAAAATTAAGT+AGG - chr4_1:53903569-53903588 Msa0555890:intron 15.0%
!! AATGCTATTAAAATTAAGTA+GGG - chr4_1:53903570-53903589 Msa0555890:intron 15.0%
!!! TTTGTTTTCTTTAATGAATT+AGG - chr4_1:53904824-53904843 Msa0555890:intron 15.0%
!!! AAGAATTTTTACTTCATTTT+TGG + chr4_1:53905290-53905309 None:intergenic 15.0%
!! TAGGTTATATATATTACCAT+AGG + chr4_1:53902910-53902929 None:intergenic 20.0%
!!! AGTAAAATTTTGTGACAATT+GGG + chr4_1:53903072-53903091 None:intergenic 20.0%
!! AAACAAATTTGAATTGAGTA+GGG + chr4_1:53903219-53903238 None:intergenic 20.0%
!! TAAACAAATTTGAATTGAGT+AGG + chr4_1:53903220-53903239 None:intergenic 20.0%
!! AAATTAATCATAGCTTAAAG+CGG - chr4_1:53904563-53904582 Msa0555890:CDS 20.0%
!! AAGCAAATTGAAATCTAATT+TGG + chr4_1:53904683-53904702 None:intergenic 20.0%
!! ATATATATCATCAATTACTG+AGG + chr4_1:53904992-53905011 None:intergenic 20.0%
!! GATGATATATATGATGTTTA+TGG - chr4_1:53905000-53905019 Msa0555890:intron 20.0%
!! AATATTGAGATGTGAAAATT+TGG + chr4_1:53905081-53905100 None:intergenic 20.0%
!! ATTTGAATTAAAATTCTAGG+TGG - chr4_1:53905140-53905159 Msa0555890:CDS 20.0%
!! TTTGAATTAAAATTCTAGGT+GGG - chr4_1:53905141-53905160 Msa0555890:CDS 20.0%
!!! CATATTCTTGTAAATTGTTT+TGG - chr4_1:53905420-53905439 Msa0555890:intron 20.0%
!!! CTATGAAGTTTTCAATTATT+GGG - chr4_1:53906142-53906161 Msa0555890:CDS 20.0%
! CCAAGACATATTGAATAAAA+AGG + chr4_1:53902885-53902904 None:intergenic 25.0%
!! CCTTTTTATTCAATATGTCT+TGG - chr4_1:53902882-53902901 Msa0555890:three_prime_UTR 25.0%
!! GTAAAATTTTGTGACAATTG+GGG + chr4_1:53903071-53903090 None:intergenic 25.0%
!! GAGTAAAATTTTGTGACAAT+TGG + chr4_1:53903073-53903092 None:intergenic 25.0%
! AGTGCAGAAAAACAAAATTA+GGG + chr4_1:53903186-53903205 None:intergenic 25.0%
!!! TAATTTTGTTTTTCTGCACT+GGG - chr4_1:53903186-53903205 Msa0555890:CDS 25.0%
!!! TAATCAAACTTTGTTTTAGC+AGG - chr4_1:53903508-53903527 Msa0555890:CDS 25.0%
!!! AATCAAACTTTGTTTTAGCA+GGG - chr4_1:53903509-53903528 Msa0555890:CDS 25.0%
! CACAATAATCATGTTGAAAA+TGG - chr4_1:53903836-53903855 Msa0555890:CDS 25.0%
! AAATCTAATACAAGTAGACA+AGG + chr4_1:53903955-53903974 None:intergenic 25.0%
! CTTAACCATATATTATCATG+TGG - chr4_1:53904338-53904357 Msa0555890:CDS 25.0%
! TTAATTTCTGTAGCAAGAAA+AGG + chr4_1:53904550-53904569 None:intergenic 25.0%
!! GTTTTCTTTAATGAATTAGG+TGG - chr4_1:53904827-53904846 Msa0555890:intron 25.0%
! TAGCAAAATTTCATGTATTG+TGG + chr4_1:53905190-53905209 None:intergenic 25.0%
! TTCTTCATAAACATCCAAAA+CGG + chr4_1:53905218-53905237 None:intergenic 25.0%
! TTTGGATGTTTATGAAGAAA+TGG - chr4_1:53905219-53905238 Msa0555890:CDS 25.0%
! ATTGATGTGCTTTATAAAGA+TGG - chr4_1:53905995-53906014 Msa0555890:intron 25.0%
!! GCTATGAAGTTTTCAATTAT+TGG - chr4_1:53906141-53906160 Msa0555890:CDS 25.0%
TTCAGTGTAATTACAAGTGT+CGG + chr4_1:53903006-53903025 None:intergenic 30.0%
!! TACAAGGTCGCTATTTTATA+GGG + chr4_1:53903601-53903620 None:intergenic 30.0%
!! TTACAAGGTCGCTATTTTAT+AGG + chr4_1:53903600-53903619 None:intergenic 30.0%
CAGTGCAGAAAAACAAAATT+AGG + chr4_1:53903187-53903206 None:intergenic 30.0%
!!! CTAATTTTGTTTTTCTGCAC+TGG - chr4_1:53903185-53903204 Msa0555890:CDS 30.0%
CAAGATTAGAACACTATGAA+AGG + chr4_1:53903446-53903465 None:intergenic 30.0%
!! TTACAAGGTCGCTATTTTAT+AGG - chr4_1:53903600-53903619 Msa0555890:intron 30.0%
!! TACAAGGTCGCTATTTTATA+GGG - chr4_1:53903601-53903620 Msa0555890:intron 30.0%
AATTCCTGCACATTAAAGTA+GGG + chr4_1:53903644-53903663 None:intergenic 30.0%
AAATTCCTGCACATTAAAGT+AGG + chr4_1:53903645-53903664 None:intergenic 30.0%
TAGTGCTAAAAGAAAAAGTG+AGG - chr4_1:53903687-53903706 Msa0555890:intron 30.0%
! GGTTTGTGACATTTTTGTTA+GGG + chr4_1:53903724-53903743 None:intergenic 30.0%
! ATTGATTCAATCCAACGTTT+GGG - chr4_1:53903764-53903783 Msa0555890:CDS 30.0%
ATTCAATTGCACTTGTCTTT+AGG - chr4_1:53903885-53903904 Msa0555890:intron 30.0%
GTTAGTGTCTGTATCATATT+CGG - chr4_1:53904196-53904215 Msa0555890:intron 30.0%
TCAATATATGCTATGAAGCA+CGG + chr4_1:53904237-53904256 None:intergenic 30.0%
TAGCATATATTGATCTCCAT+CGG - chr4_1:53904244-53904263 Msa0555890:intron 30.0%
GAGAACCACATGATAATATA+TGG + chr4_1:53904346-53904365 None:intergenic 30.0%
AACTTCAAAGAAACACTTGT+TGG - chr4_1:53904400-53904419 Msa0555890:CDS 30.0%
AAACAACATCAGAAAGAAGT+TGG - chr4_1:53904706-53904725 Msa0555890:intron 30.0%
TTCTTTAATGAATTAGGTGG+TGG - chr4_1:53904830-53904849 Msa0555890:intron 30.0%
! GAATAGTTGAAGCTTACTTT+TGG - chr4_1:53904896-53904915 Msa0555890:intron 30.0%
!! TGAAGCTTACTTTTGGATAT+TGG - chr4_1:53904903-53904922 Msa0555890:intron 30.0%
!! GAAGCTTACTTTTGGATATT+GGG - chr4_1:53904904-53904923 Msa0555890:intron 30.0%
! TTTTCACTCAAGCAATTGAA+AGG - chr4_1:53905043-53905062 Msa0555890:intron 30.0%
!! ATTTTGCTATAAAGCCGTTT+TGG - chr4_1:53905201-53905220 Msa0555890:CDS 30.0%
TGCACAATAGATGAAAAGAT+TGG - chr4_1:53905449-53905468 Msa0555890:intron 30.0%
!!! ATTGACAATTTTGGGGTTAT+TGG + chr4_1:53905640-53905659 None:intergenic 30.0%
!!! ATGAAGCATTGACAATTTTG+GGG + chr4_1:53905647-53905666 None:intergenic 30.0%
!!! GATGAAGCATTGACAATTTT+GGG + chr4_1:53905648-53905667 None:intergenic 30.0%
!!! AGATGAAGCATTGACAATTT+TGG + chr4_1:53905649-53905668 None:intergenic 30.0%
! ATCTAAAATTGCTAGACTCA+TGG - chr4_1:53905665-53905684 Msa0555890:intron 30.0%
TCTTCTTATGCCTATTTCAA+TGG + chr4_1:53906203-53906222 None:intergenic 30.0%
!! AGAAATCGGAGTTGGTATTA+GGG + chr4_1:53902822-53902841 None:intergenic 35.0%
AAATTACCACCCCAACATTT+GGG - chr4_1:53902855-53902874 Msa0555890:three_prime_UTR 35.0%
TCAATATGTCTTGGAACCTA+TGG - chr4_1:53902891-53902910 Msa0555890:three_prime_UTR 35.0%
AAGTAGGGACTAAACTTACA+AGG + chr4_1:53903585-53903604 None:intergenic 35.0%
AAGTAGGGACTAAACTTACA+AGG - chr4_1:53903585-53903604 Msa0555890:intron 35.0%
TTAACCCTACTTTAATGTGC+AGG - chr4_1:53903637-53903656 Msa0555890:intron 35.0%
! GGGTTTGTGACATTTTTGTT+AGG + chr4_1:53903725-53903744 None:intergenic 35.0%
AAAATGTCACAAACCCTTTG+AGG - chr4_1:53903729-53903748 Msa0555890:CDS 35.0%
AAATGTCACAAACCCTTTGA+GGG - chr4_1:53903730-53903749 Msa0555890:CDS 35.0%
! TCAAATTAGCTTCCCTCAAA+GGG + chr4_1:53903745-53903764 None:intergenic 35.0%
! ATCAAATTAGCTTCCCTCAA+AGG + chr4_1:53903746-53903765 None:intergenic 35.0%
! GATTGATTCAATCCAACGTT+TGG - chr4_1:53903763-53903782 Msa0555890:CDS 35.0%
CAATGTCATGCTCAAAATGA+TGG + chr4_1:53903796-53903815 None:intergenic 35.0%
! CATCATTTTGAGCATGACAT+TGG - chr4_1:53903794-53903813 Msa0555890:CDS 35.0%
AGACAAGTGCAATTGAATGA+AGG + chr4_1:53903883-53903902 None:intergenic 35.0%
! CACTTGTCTTTAGGTTACTA+AGG - chr4_1:53903894-53903913 Msa0555890:intron 35.0%
! TTTAGGTTACTAAGGCAACA+AGG - chr4_1:53903902-53903921 Msa0555890:intron 35.0%
! ACAAGGATATCACATTTTGC+CGG - chr4_1:53903919-53903938 Msa0555890:intron 35.0%
TAATACAAGTAGACAAGGTG+GGG + chr4_1:53903950-53903969 None:intergenic 35.0%
CTAATACAAGTAGACAAGGT+GGG + chr4_1:53903951-53903970 None:intergenic 35.0%
TCTAATACAAGTAGACAAGG+TGG + chr4_1:53903952-53903971 None:intergenic 35.0%
! TACTCTTTTTAGATGTGTCC+TGG - chr4_1:53904079-53904098 Msa0555890:CDS 35.0%
!! ATCTCCATCGGTTTAATAAG+AGG - chr4_1:53904256-53904275 Msa0555890:intron 35.0%
GAAACCTCTTATTAAACCGA+TGG + chr4_1:53904263-53904282 None:intergenic 35.0%
AAGAAGTTCAAGAATGAGCA+AGG - chr4_1:53904376-53904395 Msa0555890:CDS 35.0%
ATTGAGTGGTCATCATTTCA+AGG + chr4_1:53904522-53904541 None:intergenic 35.0%
AAGAAAAGGACTCAATTGAG+TGG + chr4_1:53904536-53904555 None:intergenic 35.0%
GCATTACATTTCCACTTATG+AGG - chr4_1:53904621-53904640 Msa0555890:CDS 35.0%
! ATAGATGAAAAGATTGGTCC+AGG - chr4_1:53905455-53905474 Msa0555890:intron 35.0%
! ACCAACAATAGAAGAGTACA+TGG - chr4_1:53905518-53905537 Msa0555890:intron 35.0%
GCTCTTGGAATAGTAAATTC+TGG - chr4_1:53905540-53905559 Msa0555890:intron 35.0%
!! CTACAGAAGAGGTTTTTCAA+TGG - chr4_1:53905610-53905629 Msa0555890:intron 35.0%
GGATGATATTGTTTCCAATG+AGG - chr4_1:53905686-53905705 Msa0555890:intron 35.0%
TTTGTGCTTGATTACCTCAT+TGG + chr4_1:53905703-53905722 None:intergenic 35.0%
! AATTAGTTTGAGCAAGAGAG+AGG - chr4_1:53905794-53905813 Msa0555890:intron 35.0%
! ATTAGTTTGAGCAAGAGAGA+GGG - chr4_1:53905795-53905814 Msa0555890:intron 35.0%
ACAAATTGAGAACTCGCATA+AGG + chr4_1:53905967-53905986 None:intergenic 35.0%
GTCATTCTTTGTTGATCCTT+GGG + chr4_1:53906038-53906057 None:intergenic 35.0%
TGTCATTCTTTGTTGATCCT+TGG + chr4_1:53906039-53906058 None:intergenic 35.0%
TCCATGTCCAATATGAGTAA+AGG - chr4_1:53906078-53906097 Msa0555890:intron 35.0%
CCATGTCCAATATGAGTAAA+GGG - chr4_1:53906079-53906098 Msa0555890:intron 35.0%
CAAAGTCCCTTTACTCATAT+TGG + chr4_1:53906088-53906107 None:intergenic 35.0%
GACAAACTAGCCATTGAAAT+AGG - chr4_1:53906190-53906209 Msa0555890:CDS 35.0%
!! GAAATCGGAGTTGGTATTAG+GGG + chr4_1:53902821-53902840 None:intergenic 40.0%
!! GAGAAATCGGAGTTGGTATT+AGG + chr4_1:53902823-53902842 None:intergenic 40.0%
CAAATTACCACCCCAACATT+TGG - chr4_1:53902854-53902873 Msa0555890:three_prime_UTR 40.0%
AATTACCACCCCAACATTTG+GGG - chr4_1:53902856-53902875 Msa0555890:three_prime_UTR 40.0%
GATGGTATAATCCCAAACGT+TGG + chr4_1:53903778-53903797 None:intergenic 40.0%
! CAAGGATATCACATTTTGCC+GGG - chr4_1:53903920-53903939 Msa0555890:intron 40.0%
GAGTAGTGTTCGTGTTTCAT+AGG + chr4_1:53904064-53904083 None:intergenic 40.0%
ATGAAGCACGGATACAAACA+TGG + chr4_1:53904225-53904244 None:intergenic 40.0%
!! AACACTTGTTGGTGATGTTG+AGG - chr4_1:53904411-53904430 Msa0555890:CDS 40.0%
!! ACACTTGTTGGTGATGTTGA+GGG - chr4_1:53904412-53904431 Msa0555890:CDS 40.0%
TGTATGAAGCCACACATATG+AGG - chr4_1:53904446-53904465 Msa0555890:CDS 40.0%
TCCATGAATCCTCATATGTG+TGG + chr4_1:53904458-53904477 None:intergenic 40.0%
ACATATGAGGATTCATGGAG+AGG - chr4_1:53904459-53904478 Msa0555890:CDS 40.0%
AGCAAAGTTGCATCATGAGA+GGG + chr4_1:53904652-53904671 None:intergenic 40.0%
TAGCAAAGTTGCATCATGAG+AGG + chr4_1:53904653-53904672 None:intergenic 40.0%
ACTATTCTGTTGCGAGCAAA+AGG + chr4_1:53904883-53904902 None:intergenic 40.0%
TTTGAGCCTCAATACTCTGT+TGG - chr4_1:53904934-53904953 Msa0555890:intron 40.0%
!! CTTCTACCAACAGAGTATTG+AGG + chr4_1:53904943-53904962 None:intergenic 40.0%
! CTCAATACTCTGTTGGTAGA+AGG - chr4_1:53904941-53904960 Msa0555890:intron 40.0%
TGGTAGAAGGATAATGACCA+AGG - chr4_1:53904954-53904973 Msa0555890:intron 40.0%
AATGACCAAGGTGATATCCT+TGG - chr4_1:53904966-53904985 Msa0555890:intron 40.0%
ATCATCAATTACTGAGGCCA+AGG + chr4_1:53904986-53905005 None:intergenic 40.0%
TAGGTGGGATATAAGTTGCA+TGG - chr4_1:53905156-53905175 Msa0555890:CDS 40.0%
TGAAGAAATGGAGCAAGAGA+TGG - chr4_1:53905231-53905250 Msa0555890:CDS 40.0%
CATACTTTACTGAGGCTCAA+TGG - chr4_1:53905478-53905497 Msa0555890:intron 40.0%
GCCATGTACTCTTCTATTGT+TGG + chr4_1:53905522-53905541 None:intergenic 40.0%
! ATAGAAGAGTACATGGCTCT+TGG - chr4_1:53905525-53905544 Msa0555890:intron 40.0%
ATCACAACAACATCCTTCGT+TGG - chr4_1:53905573-53905592 Msa0555890:intron 40.0%
AACAACATCCTTCGTTGGAA+TGG - chr4_1:53905578-53905597 Msa0555890:intron 40.0%
ACAACATCCTTCGTTGGAAT+GGG - chr4_1:53905579-53905598 Msa0555890:intron 40.0%
GAGTGCTACATGAATGAACA+TGG - chr4_1:53905836-53905855 Msa0555890:intron 40.0%
CAAGACAAGTCACAAATGCT+TGG - chr4_1:53905891-53905910 Msa0555890:intron 40.0%
ACAAGTCACAAATGCTTGGA+AGG - chr4_1:53905895-53905914 Msa0555890:intron 40.0%
TGCTTGGAAGGATATCAATG+AGG - chr4_1:53905907-53905926 Msa0555890:intron 40.0%
GATTGCTATACTAACTCCCA+AGG - chr4_1:53906019-53906038 Msa0555890:intron 40.0%
CCCTTTACTCATATTGGACA+TGG + chr4_1:53906082-53906101 None:intergenic 40.0%
ACGTCTAGAGAAATCGGAGT+TGG + chr4_1:53902830-53902849 None:intergenic 45.0%
CTCCGATTTCTCTAGACGTT+CGG - chr4_1:53902831-53902850 Msa0555890:three_prime_UTR 45.0%
TGCCGAACGTCTAGAGAAAT+CGG + chr4_1:53902836-53902855 None:intergenic 45.0%
ATTACCACCCCAACATTTGG+GGG - chr4_1:53902857-53902876 Msa0555890:three_prime_UTR 45.0%
AAAAGGTCCCCCCAAATGTT+GGG + chr4_1:53902868-53902887 None:intergenic 45.0%
AAAAAGGTCCCCCCAAATGT+TGG + chr4_1:53902869-53902888 None:intergenic 45.0%
TATGAAGCACAGACACTCCT+CGG - chr4_1:53902928-53902947 Msa0555890:CDS 45.0%
GCCACACATATGAGGATTCA+TGG - chr4_1:53904454-53904473 Msa0555890:CDS 45.0%
GGTCATCATTTCAAGGTGTG+AGG + chr4_1:53904515-53904534 None:intergenic 45.0%
CTAGGCAAGTTCTTGAAGAG+TGG + chr4_1:53904589-53904608 None:intergenic 45.0%
CACTCTTCAAGAACTTGCCT+AGG - chr4_1:53904587-53904606 Msa0555890:CDS 45.0%
AGAGGGATCTTCCTCATAAG+TGG + chr4_1:53904635-53904654 None:intergenic 45.0%
! GTGTCTGAAACGTCTCGAAT+AGG + chr4_1:53904782-53904801 None:intergenic 45.0%
AATGGAGCAAGAGATGGTCA+AGG - chr4_1:53905237-53905256 Msa0555890:CDS 45.0%
AATGCATCCCATTCCAACGA+AGG + chr4_1:53905589-53905608 None:intergenic 45.0%
CATGTAGCACTCAATAGCAG+AGG + chr4_1:53905828-53905847 None:intergenic 45.0%
! AGAGGCTTTGGCACTTGAAT+TGG + chr4_1:53905944-53905963 None:intergenic 45.0%
TTGAGAACTCGCATAAGGAG+AGG + chr4_1:53905962-53905981 None:intergenic 45.0%
!!! TATAAGTAAATTTTTTTTAT+AGG - chr4_1:53903540-53903559 Msa0555890:intron 5.0%
!!! ATAAGTAAATTTTTTTTATA+GGG - chr4_1:53903541-53903560 Msa0555890:intron 5.0%
!! ATAAAAAAATAAAAAAAACA+AGG + chr4_1:53905326-53905345 None:intergenic 5.0%
!! AAAATATAAATTTAAAAGAT+AGG + chr4_1:53905743-53905762 None:intergenic 5.0%
!! TCGGAGTTGGTATTAGGGGT+TGG + chr4_1:53902817-53902836 None:intergenic 50.0%
TTACCACCCCAACATTTGGG+GGG - chr4_1:53902858-53902877 Msa0555890:three_prime_UTR 50.0%
AAAGGTCCCCCCAAATGTTG+GGG + chr4_1:53902867-53902886 None:intergenic 50.0%
!! GAGGAGTGTCTGTGCTTCAT+AGG + chr4_1:53902929-53902948 None:intergenic 50.0%
!! TACAAGTGTCGGTGTCGTGT+TGG + chr4_1:53902995-53903014 None:intergenic 50.0%
TAGACAAGGTGGGGTTTACC+CGG + chr4_1:53903941-53903960 None:intergenic 50.0%
ATCATGTGTCAGAGTCGCGT+CGG + chr4_1:53904130-53904149 None:intergenic 50.0%
CAAGAACTTGCCTAGGCTAC+TGG - chr4_1:53904594-53904613 Msa0555890:CDS 50.0%
TAATGCCTTGCCAGTAGCCT+AGG + chr4_1:53904607-53904626 None:intergenic 50.0%
TGAGGCCAAGGATATCACCT+TGG + chr4_1:53904974-53904993 None:intergenic 50.0%
GAGCAAGAGATGGTCAAGGA+AGG - chr4_1:53905241-53905260 Msa0555890:CDS 50.0%
GGTCCAGGCATACTTTACTG+AGG - chr4_1:53905470-53905489 Msa0555890:intron 50.0%
GAGCCTCAGTAAAGTATGCC+TGG + chr4_1:53905476-53905495 None:intergenic 50.0%
GGGATGCATTGCTACAGAAG+AGG - chr4_1:53905599-53905618 Msa0555890:intron 50.0%
TTTGAGCAAGAGAGAGGGCA+TGG - chr4_1:53905800-53905819 Msa0555890:intron 50.0%
ACTCGCATAAGGAGAGGCTT+TGG + chr4_1:53905956-53905975 None:intergenic 50.0%
GCACAGACACTCCTCGGATT+AGG - chr4_1:53902934-53902953 Msa0555890:CDS 55.0%
!! TGTCGGTGTCGTGTTGGTGT+CGG + chr4_1:53902989-53903008 None:intergenic 55.0%
AACGACACGTGTTCGACACC+AGG + chr4_1:53904100-53904119 None:intergenic 55.0%
ACTTGCCTAGGCTACTGGCA+AGG - chr4_1:53904599-53904618 Msa0555890:CDS 55.0%
! TCCTCGGATTAGGCGTGTCC+CGG - chr4_1:53902944-53902963 Msa0555890:CDS 60.0%
GATTAGGCGTGTCCCGGTGT+CGG - chr4_1:53902950-53902969 Msa0555890:CDS 60.0%
ACACGACACGTGTCCGACAC+CGG + chr4_1:53902966-53902985 None:intergenic 60.0%
! TGTCAGAGTCGCGTCGGTGT+TGG + chr4_1:53904124-53904143 None:intergenic 60.0%
GGTCCCCCCAAATGTTGGGG+TGG + chr4_1:53902864-53902883 None:intergenic 65.0%
ACCGGGACACGCCTAATCCG+AGG + chr4_1:53902948-53902967 None:intergenic 65.0%
CACGACACGTGTCCGACACC+GGG + chr4_1:53902965-53902984 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 53902781 53906235 53902781 ID=Msa0555890;Name=Msa0555890
chr4_1 mRNA 53902781 53906235 53902781 ID=Msa0555890-mRNA-1;Parent=Msa0555890;Name=Msa0555890-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.66|0.57|1|1|1|7|142|562
chr4_1 exon 53902781 53903216 53902781 ID=Msa0555890-mRNA-1:exon:15804;Parent=Msa0555890-mRNA-1
chr4_1 exon 53903309 53903557 53903309 ID=Msa0555890-mRNA-1:exon:15803;Parent=Msa0555890-mRNA-1
chr4_1 exon 53903719 53903857 53903719 ID=Msa0555890-mRNA-1:exon:15802;Parent=Msa0555890-mRNA-1
chr4_1 exon 53903952 53904170 53903952 ID=Msa0555890-mRNA-1:exon:15801;Parent=Msa0555890-mRNA-1
chr4_1 exon 53904276 53904648 53904276 ID=Msa0555890-mRNA-1:exon:15800;Parent=Msa0555890-mRNA-1
chr4_1 exon 53905075 53905357 53905075 ID=Msa0555890-mRNA-1:exon:15799;Parent=Msa0555890-mRNA-1
chr4_1 exon 53906104 53906235 53906104 ID=Msa0555890-mRNA-1:exon:15798;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53906104 53906235 53906104 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53905075 53905357 53905075 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53904276 53904648 53904276 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53903952 53904170 53903952 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53903719 53903857 53903719 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53903309 53903557 53903309 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 CDS 53902923 53903216 53902923 ID=Msa0555890-mRNA-1:cds;Parent=Msa0555890-mRNA-1
chr4_1 three_prime_UTR 53902781 53902922 53902781 ID=Msa0555890-mRNA-1:three_prime_utr;Parent=Msa0555890-mRNA-1
Gene Sequence

>Msa0555890

ATGTCGGTTTCAGTAACATCATCACTAGTTGCTCCAACCCCTAATACCAACTCCGATTTCTCTAGACGTTCGGCAAATTACCACCCCAACATTTGGGGGGACCTTTTTATTCAATATGTCTTGGAACCTATGGAATTTGATGAAATAATGAAGCAGATTATAGTGCTAAAAGAAAAAGTGAGGCAGATGCTAGTCCCTAACAAAAATGTCACAAACCCTTTGAGGGAAGCTAATTTGATTGATTCAATCCAACGTTTGGGATTATACCATCATTTTGAGCATGACATTGGAGAATTATTGCGACATATTCACAATAATCATGTTGAAAATGGAACAATAACTCTTAACGAAGACCTTCATTCAATTGCACTTGTCTTTAGGTTACTAAGGCAACAAGGATATCACATTTTGCCGGATGCTTTTAAGAAGTTCAAGAATGAGCAAGGAAACTTCAAAGAAACACTTGTTGGTGATGTTGAGGGAATGCTAAGTTTGTATGAAGCCACACATATGAGGATTCATGGAGAGGATATATTAGATGAAGCACTTTCTTTCACTTCCTCACACCTTGAAATGATGACCACTCAATTGAGTCCTTTTCTTGCTACAGAAATTAATCATAGCTTAAAGCGGCCACTCTTCAAGAACTTGCCTAGGCTACTGGCAAGGCATTACATTTCCACTTATGAGGAAGATCCCTCTCATGATGCAACTTTGCTATTACTTGCCAAATTAGATTTCAATTTGCTTCAAAAACAACATCAGAAAGAAGTTGGCGATATTTCAAAGTGGTGGAAAGATTTAGATTTTGCAACAAAACTACCTTTTGCTCGCAACAGAATAGTTGAAGCTTACTTTTGGATATTGGGAGTGTATTTTGAGCCTCAATACTCTGTTGGTAGAAGGATAATGACCAAGGTGATATCCTTGGCCTCAGTAATTGATGATATATATGATGTTTATGGTACAATAGAAGAGCTACAACTTTTCACTCAAGCAATTGAAAGGTGGGATATAAGTTGCATGGATTTTCTACCACAATACATGAAATTTTGCTATAAAGCCGTTTTGGATGTTTATGAAGAAATGGAGCAAGAGATGGTCAAGGAAGGCAGAGCATTTTGTGTAACATATGCCAAAAATGAAATGAAAAGATTGGTCCAGGCATACTTTACTGAGGCTCAATGGTTCAGTAGAAAATATATACCAACAATAGAAGAGTACATGGCTCTTGGAATAGTAAATTCTGGTTACTATCTAATCACAACAACATCCTTCGTTGGAATGGGATGCATTGCTACAGAAGAGGTTTTTCAATGGTTAACCAATAACCCCAAAATTGTCAATGCTTCATCTAAAATTGCTAGACTCATGGATGATATTGTTTCCAATGAGTTTGAGCAAGAGAGAGGGCATGGTGCCTCTGCTATTGAGTGCTACATGAATGAACATGGTGTGTCTAGAGAAGATGCAGTTAATGAACTTTCAAGACAAGTCACAAATGCTTGGAAGGATATCAATGAGGAACTTCTTGATCCAATTCAAGTGCCAAAGCCTCTCCTTATGCGAGTTCTCAATTTGTCACGTGTCATTGATGTGCTTTATAAAGATGGAGATTGCTATACTAACTCCCAAGGATCAACAAAGAATGACATAATATCTATCTTGTTGAATCCATGTCCAATATGA
Protein sequence

>Msa0555890

MSVSVTSSLVAPTPNTNSDFSRRSANYHPNIWGDLFIQYVLEPMEFDEIMKQIIVLKEKVRQMLVPNKNVTNPLREANLIDSIQRLGLYHHFEHDIGELLRHIHNNHVENGTITLNEDLHSIALVFRLLRQQGYHILPDAFKKFKNEQGNFKETLVGDVEGMLSLYEATHMRIHGEDILDEALSFTSSHLEMMTTQLSPFLATEINHSLKRPLFKNLPRLLARHYISTYEEDPSHDATLLLLAKLDFNLLQKQHQKEVGDISKWWKDLDFATKLPFARNRIVEAYFWILGVYFEPQYSVGRRIMTKVISLASVIDDIYDVYGTIEELQLFTQAIERWDISCMDFLPQYMKFCYKAVLDVYEEMEQEMVKEGRAFCVTYAKNEMKRLVQAYFTEAQWFSRKYIPTIEEYMALGIVNSGYYLITTTSFVGMGCIATEEVFQWLTNNPKIVNASSKIARLMDDIVSNEFEQERGHGASAIECYMNEHGVSREDAVNELSRQVTNAWKDINEELLDPIQVPKPLLMRVLNLSRVIDVLYKDGDCYTNSQGSTKNDIISILLNPCPI*