AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0724140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724140 MtrunA17_Chr5g0415191 96.203 316 10 2 1 316 1 314 0.0 617
Msa0724140 MtrunA17_Chr4g0055931 48.780 205 65 5 3 188 1 184 4.36e-56 184
Msa0724140 MtrunA17_Chr2g0331031 48.558 208 60 6 3 183 1 188 1.57e-52 176
Msa0724140 MtrunA17_Chr4g0059701 55.721 201 55 7 24 209 8 189 4.90e-49 165
Msa0724140 MtrunA17_Chr6g0488471 46.893 177 68 5 3 168 1 162 3.36e-45 155
Msa0724140 MtrunA17_Chr8g0389241 41.429 210 80 8 5 188 4 196 6.17e-39 138
Msa0724140 MtrunA17_Chr3g0092621 74.118 85 19 2 103 185 84 167 4.11e-36 128
Msa0724140 MtrunA17_Chr3g0104071 68.966 87 25 1 92 176 134 220 4.84e-34 127
Msa0724140 MtrunA17_Chr4g0062741 72.603 73 20 0 97 169 132 204 2.51e-33 124
Msa0724140 MtrunA17_Chr1g0162081 71.233 73 21 0 97 169 116 188 1.32e-32 122
Msa0724140 MtrunA17_Chr5g0429521 70.513 78 23 0 97 174 126 203 1.67e-32 122
Msa0724140 MtrunA17_Chr3g0135671 40.449 178 75 5 1 167 1 158 3.27e-32 119
Msa0724140 MtrunA17_Chr7g0241761 67.949 78 25 0 97 174 134 211 3.86e-31 119
Msa0724140 MtrunA17_Chr1g0210471 68.421 76 24 0 94 169 115 190 7.81e-31 116
Msa0724140 MtrunA17_Chr5g0436501 50.847 118 53 2 90 205 112 226 8.29e-30 114
Msa0724140 MtrunA17_Chr6g0452791 58.537 82 24 1 103 184 80 151 4.06e-25 99.4
Msa0724140 MtrunA17_Chr5g0440731 40.385 104 56 2 97 194 99 202 4.24e-20 88.6
Msa0724140 MtrunA17_Chr5g0409841 43.243 111 49 4 74 170 51 161 9.09e-20 86.3
Msa0724140 MtrunA17_Chr5g0440741 35.032 157 90 6 105 253 102 254 1.52e-19 87.0
Msa0724140 MtrunA17_Chr5g0409831 65.455 55 19 0 96 150 70 124 1.63e-18 82.8
Msa0724140 MtrunA17_Chr8g0365011 47.436 78 27 2 105 168 94 171 6.84e-16 76.6
Msa0724140 MtrunA17_Chr8g0364731 61.017 59 21 1 112 168 10 68 1.09e-15 73.9
Msa0724140 MtrunA17_Chr5g0432921 39.423 104 48 3 80 169 78 180 4.65e-13 67.4
Msa0724140 MtrunA17_Chr1g0192061 48.571 70 32 2 105 171 63 131 8.66e-13 65.9
Msa0724140 MtrunA17_Chr5g0432941 49.254 67 31 2 105 169 115 180 3.16e-12 65.1
Msa0724140 MtrunA17_Chr3g0113121 45.833 72 32 1 105 169 97 168 6.41e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724140 AT1G70000.1 49.235 327 87 12 3 316 1 261 1.27e-74 230
Msa0724140 AT1G70000.2 49.235 327 87 12 3 316 1 261 1.27e-74 230
Msa0724140 AT5G47390.1 52.973 185 52 3 3 172 1 165 1.40e-56 187
Msa0724140 AT5G61620.1 46.154 182 82 5 1 174 6 179 8.22e-47 160
Msa0724140 AT3G16350.1 42.795 229 71 5 3 183 1 217 2.70e-44 155
Msa0724140 AT1G74840.2 53.608 194 54 5 20 197 15 188 6.27e-44 150
Msa0724140 AT1G74840.1 56.180 178 44 4 20 183 15 172 1.38e-43 150
Msa0724140 AT1G19000.2 52.551 196 51 6 20 193 13 188 3.17e-43 150
Msa0724140 AT1G19000.1 52.551 196 51 6 20 193 13 188 3.17e-43 150
Msa0724140 AT5G56840.1 46.328 177 83 5 3 177 1 167 3.68e-42 146
Msa0724140 AT5G58900.1 64.773 88 29 1 93 178 129 216 1.83e-33 125
Msa0724140 AT1G49010.1 73.973 73 19 0 97 169 128 200 3.44e-33 124
Msa0724140 AT5G08520.1 69.863 73 22 0 97 169 112 184 7.72e-32 120
Msa0724140 AT2G38090.1 69.333 75 23 0 98 172 136 210 9.21e-32 120
Msa0724140 AT5G04760.1 73.611 72 19 0 98 169 94 165 3.01e-31 117
Msa0724140 AT5G01200.1 67.123 73 24 0 97 169 140 212 1.40e-29 114
Msa0724140 AT3G11280.2 60.976 82 32 0 92 173 116 197 1.51e-28 111
Msa0724140 AT3G11280.1 60.976 82 32 0 92 173 116 197 1.51e-28 111
Msa0724140 AT5G05790.2 59.756 82 33 0 92 173 120 201 1.07e-27 109
Msa0724140 AT5G05790.1 59.756 82 33 0 92 173 120 201 1.07e-27 109
Msa0724140 AT5G23650.1 56.579 76 33 0 93 168 110 185 5.17e-22 95.1
Msa0724140 AT4G09450.1 51.685 89 39 2 84 168 68 156 1.27e-21 90.9
Msa0724140 AT3G10580.2 57.143 70 28 1 99 168 91 158 1.27e-21 92.4
Msa0724140 AT3G10580.1 57.143 70 28 1 99 168 91 158 2.19e-21 92.4
Msa0724140 AT3G10590.1 50.617 81 37 2 93 171 99 178 7.39e-19 83.6

Find 168 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTAATATGATTAAGAATT+TGG 0.086398 5_1:+22407463 Msa0724140:three_prime_UTR
TCTCTACTTAATTAATTTAT+TGG 0.135934 5_1:-22407362 None:intergenic
GAGAGATGATTTGCGAGATT+TGG 0.170674 5_1:-22405204 None:intergenic
TTAAATTGAGAAAGATAAAA+TGG 0.218744 5_1:+22407571 Msa0724140:three_prime_UTR
ATAGATTGGTAGAGAGTAAT+TGG 0.231815 5_1:+22407873 Msa0724140:three_prime_UTR
ATGAGAGAGAACAAGATAAT+TGG 0.238850 5_1:+22408440 Msa0724140:three_prime_UTR
GTGGTGGTGGTGGAGGTGTT+GGG 0.249956 5_1:-22405518 None:intergenic
GGTGGTGGTGGTGGAGGTGT+TGG 0.254614 5_1:-22405519 None:intergenic
GATTCTTGTTTCTATTTGTC+TGG 0.267237 5_1:+22407632 Msa0724140:three_prime_UTR
TGATTTATTTATACACTTGA+TGG 0.273249 5_1:+22408013 Msa0724140:three_prime_UTR
CTAAATTTCAAATATTAGTT+TGG 0.284780 5_1:+22407731 Msa0724140:three_prime_UTR
GAGTCAGATGACTAGAAATA+AGG 0.289583 5_1:-22408285 None:intergenic
ATCTCCGGCGGCGGCGGTTC+TGG 0.294270 5_1:-22405933 None:intergenic
GGGTACTGTTGGTCTCTTAT+TGG 0.298397 5_1:+22407685 Msa0724140:three_prime_UTR
GACGAATGGCTGCTACTTTC+CGG 0.303324 5_1:-22407161 None:intergenic
ATGAATCATGAGAATGATTA+TGG 0.312226 5_1:+22407442 Msa0724140:three_prime_UTR
AGCATTGGTGTCGGCCTAAT+TGG 0.315506 5_1:-22407017 None:intergenic
AGAGATGATTTGCGAGATTT+GGG 0.316990 5_1:-22405203 None:intergenic
CTGACCTCGTTTGCGTTCTC+TGG 0.320215 5_1:-22405678 None:intergenic
TGGTGGTGGTGGAGGTGTTG+GGG 0.324638 5_1:-22405517 None:intergenic
GCATATCCATCTTCACATTA+TGG 0.334586 5_1:+22406915 Msa0724140:CDS
ATTTGCGAGATTTGGGTGAT+AGG 0.341860 5_1:-22405196 None:intergenic
GGCAGTCGATCCTACTTAAC+TGG 0.346798 5_1:-22408166 None:intergenic
TGGTGGTGGAGGTGTTGGGG+TGG 0.360044 5_1:-22405514 None:intergenic
GATGGAACCTTCAACTATAA+TGG 0.360343 5_1:+22406836 Msa0724140:CDS
AAACTAGACCTATGTGGGTT+TGG 0.360671 5_1:-22408362 None:intergenic
CTAGAAACTTTGTGAAAACT+AGG 0.361627 5_1:+22405857 Msa0724140:CDS
GATGTCATCGGATGCATAAC+CGG 0.365215 5_1:-22405636 None:intergenic
ATTGTTATTGGTGGTGGTGG+TGG 0.365994 5_1:-22405534 None:intergenic
AGGTGATTAGCCGAATTAAT+TGG 0.370332 5_1:+22407517 Msa0724140:three_prime_UTR
AAAGAGTGGCCAAGCCAATT+AGG 0.378686 5_1:+22407003 Msa0724140:CDS
CTCATTACCGGCAATGTTAC+CGG 0.380041 5_1:-22406975 None:intergenic
TATTTCTCAAAATTCTCTCT+TGG 0.382024 5_1:+22407952 Msa0724140:three_prime_UTR
ATTCTTGTTTCTATTTGTCT+GGG 0.384174 5_1:+22407633 Msa0724140:three_prime_UTR
TCACATTATGGCGGCATCCC+TGG 0.390624 5_1:+22406927 Msa0724140:CDS
AACTAGACCTATGTGGGTTT+GGG 0.393060 5_1:-22408361 None:intergenic
GACTGGATCTCCGGCGGCGG+CGG 0.396452 5_1:-22405939 None:intergenic
ACTGTAACGTCGTTTGATTC+AGG 0.400440 5_1:-22408187 None:intergenic
GAGTGTGACCTTGAAGTGAT+AGG 0.402284 5_1:+22407657 Msa0724140:three_prime_UTR
TTGTAAATTGAGGTTAAAGT+TGG 0.402565 5_1:+22407310 Msa0724140:three_prime_UTR
ACTCTTTCAGCACTCATTAC+CGG 0.403021 5_1:-22406987 None:intergenic
GTCATTGAAGCTGCAACCTT+CGG 0.407621 5_1:+22407118 Msa0724140:CDS
GTGGTTGTTCATATTGAGAA+AGG 0.407804 5_1:-22405586 None:intergenic
AGGATTGTTATTGGTGGTGG+TGG 0.412317 5_1:-22405537 None:intergenic
GTTCTTGCTGGGGTTGCAAC+AGG 0.415423 5_1:+22405807 Msa0724140:CDS
TGTTGCTTGAAAATTTAGTT+TGG 0.417854 5_1:+22407262 Msa0724140:exon
AGTCAGATGACTAGAAATAA+GGG 0.419536 5_1:-22408284 None:intergenic
AGAACACAAGTTGTTCTTGC+TGG 0.422978 5_1:+22405795 Msa0724140:CDS
ATAATATAAGTAGGAGGACT+AGG 0.428357 5_1:+22407392 Msa0724140:three_prime_UTR
TAGATTGGTAGAGAGTAATT+GGG 0.428647 5_1:+22407874 Msa0724140:three_prime_UTR
AAAAGGAAGAAGATAATGTT+AGG 0.428858 5_1:+22407488 Msa0724140:three_prime_UTR
TCATCTTCCATTATAGTTGA+AGG 0.430302 5_1:-22406843 None:intergenic
TCTTGGTCCGGTAACATTGC+CGG 0.432456 5_1:+22406968 Msa0724140:CDS
CCCATTGCCCTATCACTTCA+AGG 0.434219 5_1:-22407665 None:intergenic
GATGCATAACCGGCATCGGC+GGG 0.438494 5_1:-22405626 None:intergenic
GTTGGGGTGGTTGTTTCCTC+CGG 0.439255 5_1:-22405501 None:intergenic
AAGATAATGTTAGGTTATGT+AGG 0.445982 5_1:+22407497 Msa0724140:three_prime_UTR
TAAATTGAGAAAGATAAAAT+GGG 0.445990 5_1:+22407572 Msa0724140:three_prime_UTR
CACATTATGGCGGCATCCCT+GGG 0.451781 5_1:+22406928 Msa0724140:CDS
GAACACAAGTTGTTCTTGCT+GGG 0.453529 5_1:+22405796 Msa0724140:CDS
AACAGGTTGGTAAAGGTGAT+TGG 0.456244 5_1:+22405824 Msa0724140:CDS
TTGGTCTATTTCTTGCAACT+TGG 0.457256 5_1:+22407704 Msa0724140:three_prime_UTR
ATGGCTGCAGGGAAGTATAA+TGG 0.461919 5_1:+22407215 Msa0724140:CDS
TCTAGATTATGTTCCTTACA+TGG 0.465854 5_1:+22405318 Msa0724140:five_prime_UTR
TGCTGAAATCAATCCATGTA+AGG 0.468342 5_1:-22405331 None:intergenic
AGATTGGTAGAGAGTAATTG+GGG 0.474479 5_1:+22407875 Msa0724140:three_prime_UTR
CCCATCGGAAAAGGTGTCCC+AGG 0.480895 5_1:-22406945 None:intergenic
GAGGATAATATAATATAAGT+AGG 0.483430 5_1:+22407383 Msa0724140:three_prime_UTR
GAAAGGATTGTTATTGGTGG+TGG 0.486611 5_1:-22405540 None:intergenic
CAAATATTAGTTTGGACAAG+AGG 0.487817 5_1:+22407739 Msa0724140:three_prime_UTR
AGAACTTAAGATAAATGAGT+TGG 0.488158 5_1:-22408234 None:intergenic
TTCTAATAATATGAGAGAGT+TGG 0.500580 5_1:-22408414 None:intergenic
TCTGAAAGGATTGTTATTGG+TGG 0.500851 5_1:-22405543 None:intergenic
AGATTAGCCATCTTAGAAGA+AGG 0.500963 5_1:-22407044 None:intergenic
TGGTTGATGAAGGTGTTCCT+TGG 0.504873 5_1:+22405764 Msa0724140:intron
GATGACATCGTTCACGCCTC+TGG 0.506341 5_1:+22405650 Msa0724140:CDS
AGCTTCAATGACAATGGAAA+AGG 0.506440 5_1:-22407107 None:intergenic
CCTTGAAGTGATAGGGCAAT+GGG 0.508448 5_1:+22407665 Msa0724140:three_prime_UTR
GTTGACTGGTTTGTAAATTG+AGG 0.512290 5_1:+22407300 Msa0724140:three_prime_UTR
CGTTGCATGTTCTCGAGTTG+TGG 0.513462 5_1:-22405433 None:intergenic
TTCTTGTTTCTATTTGTCTG+GGG 0.514391 5_1:+22407634 Msa0724140:three_prime_UTR
TGTGAAGATGGATATGCGGC+GGG 0.518568 5_1:-22406909 None:intergenic
GGACCAAGACCCATCGGAAA+AGG 0.520312 5_1:-22406954 None:intergenic
GCTGCCTCCTTCTTCTAAGA+TGG 0.520621 5_1:+22407037 Msa0724140:CDS
TAGCAGCCATTCGTCGTCAT+CGG 0.522877 5_1:+22407169 Msa0724140:CDS
TGGTGTCGGCCTAATTGGCT+TGG 0.524161 5_1:-22407012 None:intergenic
GGTGGTAATATCAAAGAGAC+TGG 0.526759 5_1:-22405956 None:intergenic
TTAGAAGAAGGAGGCAGCAT+TGG 0.530338 5_1:-22407032 None:intergenic
AGAACAAGATAATTGGAAGA+AGG 0.530636 5_1:+22408447 Msa0724140:three_prime_UTR
TTGCTGGGGTTGCAACAGGT+TGG 0.532980 5_1:+22405811 Msa0724140:CDS
TAAATAAATCAAGTAATGGA+TGG 0.533519 5_1:-22408001 None:intergenic
TAATATAAGTAGGAGGACTA+GGG 0.533602 5_1:+22407393 Msa0724140:three_prime_UTR
ATCGGATGCATAACCGGCAT+CGG 0.537724 5_1:-22405630 None:intergenic
TCTTGTTTCTATTTGTCTGG+GGG 0.537804 5_1:+22407635 Msa0724140:three_prime_UTR
ATAACCGGCATCGGCGGGGT+TGG 0.538106 5_1:-22405621 None:intergenic
GATTCCAACCCCGCCGATGC+CGG 0.539445 5_1:+22405617 Msa0724140:CDS
GGGTTGCAACAGGTTGGTAA+AGG 0.541687 5_1:+22405817 Msa0724140:CDS
AGAGACTGGATCTCCGGCGG+CGG 0.543258 5_1:-22405942 None:intergenic
TGTATAAATAAATCAAGTAA+TGG 0.543614 5_1:-22408005 None:intergenic
TTGTGTTCTTCCTCTGTCCA+AGG 0.544021 5_1:-22405781 None:intergenic
GGGTTGGAATCTTGTGGAGG+TGG 0.544666 5_1:-22405605 None:intergenic
GCAGGGAAGTATAATGGTGG+GGG 0.547007 5_1:+22407221 Msa0724140:CDS
ATAACCAGAACCGCCGCCGC+CGG 0.548822 5_1:+22405929 Msa0724140:CDS
TATCAAAGAGACTGGATCTC+CGG 0.548838 5_1:-22405948 None:intergenic
CTTTGATATTACCACCGATA+CGG 0.550867 5_1:+22405963 Msa0724140:CDS
TTGTTCATATTGAGAAAGGT+TGG 0.551197 5_1:-22405582 None:intergenic
TTACATACCCAAACCCACAT+AGG 0.555703 5_1:+22408354 Msa0724140:three_prime_UTR
TGATTAGCCGAATTAATTGG+TGG 0.556070 5_1:+22407520 Msa0724140:three_prime_UTR
GATAGGGCAATGGGTACTGT+TGG 0.557520 5_1:+22407674 Msa0724140:three_prime_UTR
ATGCCGCCATAATGTGAAGA+TGG 0.557994 5_1:-22406921 None:intergenic
TAAATCAAGTAATGGATGGA+AGG 0.558633 5_1:-22407997 None:intergenic
AGTGTGACCTTGAAGTGATA+GGG 0.559552 5_1:+22407658 Msa0724140:three_prime_UTR
ACTCTCTACCAATCTATGTA+CGG 0.561929 5_1:-22407867 None:intergenic
GCTGCAACCTTCGGTTCCAT+CGG 0.562274 5_1:+22407127 Msa0724140:CDS
TTGAGAAAGATAAAATGGGT+TGG 0.562383 5_1:+22407576 Msa0724140:three_prime_UTR
ATTATACTTCCCTGCAGCCA+TGG 0.565515 5_1:-22407213 None:intergenic
TCCGGTGAATGATCTTCCGA+TGG 0.572768 5_1:-22407143 None:intergenic
AAGAACAAATATTACCGTAT+CGG 0.577972 5_1:-22405977 None:intergenic
CTGCAGGGAAGTATAATGGT+GGG 0.579931 5_1:+22407219 Msa0724140:CDS
TGCAGGGAAGTATAATGGTG+GGG 0.579949 5_1:+22407220 Msa0724140:CDS
GGCGGCGGTTCTGGTTATGA+CGG 0.580334 5_1:-22405924 None:intergenic
GATAGAAACTAGACCTATGT+GGG 0.581693 5_1:-22408367 None:intergenic
GCATGACTTGCAACCTGAGT+TGG 0.584887 5_1:-22405883 None:intergenic
TGAAGATGGATATGCGGCGG+GGG 0.585757 5_1:-22406907 None:intergenic
TGACAATGGAAAAGGCTCGA+TGG 0.586187 5_1:-22407099 None:intergenic
TGATCTTCCGATGGAACCGA+AGG 0.587262 5_1:-22407134 None:intergenic
TGCTCACAGTGCGGCAACAA+CGG 0.587587 5_1:+22405410 Msa0724140:CDS
TGATAGAAACTAGACCTATG+TGG 0.588500 5_1:-22408368 None:intergenic
GATAATATAATATAAGTAGG+AGG 0.590162 5_1:+22407386 Msa0724140:three_prime_UTR
AACAAATATTACCGTATCGG+TGG 0.590836 5_1:-22405974 None:intergenic
TCGGCGGGGTTGGAATCTTG+TGG 0.592119 5_1:-22405611 None:intergenic
GAAAACTAGGACACCAACTC+AGG 0.594289 5_1:+22405870 Msa0724140:CDS
ACCTTGAAGTGATAGGGCAA+TGG 0.597131 5_1:+22407664 Msa0724140:three_prime_UTR
GGATGCATAACCGGCATCGG+CGG 0.606496 5_1:-22405627 None:intergenic
ATAAGTAGGAGGACTAGGGA+GGG 0.607813 5_1:+22407397 Msa0724140:three_prime_UTR
AATAAATTAATTAAGTAGAG+AGG 0.609464 5_1:+22407364 Msa0724140:three_prime_UTR
GGTTGCAGCTTCAATGACAA+TGG 0.610541 5_1:-22407113 None:intergenic
AGAGGCGTGAACGATGTCAT+CGG 0.614412 5_1:-22405648 None:intergenic
AACACAAGTTGTTCTTGCTG+GGG 0.614508 5_1:+22405797 Msa0724140:CDS
GCGTTCTCTGGATCGACCAG+AGG 0.617091 5_1:-22405666 None:intergenic
ATGAAGCCGATGACGACGAA+TGG 0.619919 5_1:-22407175 None:intergenic
TGAAGGTGTTCCTTGGACAG+AGG 0.622183 5_1:+22405771 Msa0724140:intron
GAAGGAGGCAGCATTGGTGT+CGG 0.622641 5_1:-22407026 None:intergenic
TCGAGAACATGCAACGACGG+TGG 0.622712 5_1:+22405440 Msa0724140:CDS
GGTTCTGGTTATGACGGCGG+AGG 0.625551 5_1:-22405918 None:intergenic
ATGTGAAGATGGATATGCGG+CGG 0.625832 5_1:-22406910 None:intergenic
GGACTAGGGAGGGACTAGAG+AGG 0.625911 5_1:+22407407 Msa0724140:three_prime_UTR
TCCATCGGAAGATCATTCAC+CGG 0.635009 5_1:+22407142 Msa0724140:CDS
GTTACCGGACCAAGACCCAT+CGG 0.636658 5_1:-22406960 None:intergenic
TTTGGTGTTCGACTCACCGG+AGG 0.638984 5_1:+22405485 Msa0724140:CDS
TATAAGTAGGAGGACTAGGG+AGG 0.640458 5_1:+22407396 Msa0724140:three_prime_UTR
CCATCGGAAAAGGTGTCCCA+GGG 0.645708 5_1:-22406944 None:intergenic
AACTCGAGAACATGCAACGA+CGG 0.649079 5_1:+22405437 Msa0724140:CDS
GCTGCAGGGAAGTATAATGG+TGG 0.654189 5_1:+22407218 Msa0724140:CDS
GGTAATGAGTGCTGAAAGAG+TGG 0.656043 5_1:+22406989 Msa0724140:CDS
CGATCCAGAGAACGCAAACG+AGG 0.663587 5_1:+22405674 Msa0724140:CDS
GTTGGTAAAGGTGATTGGAG+AGG 0.666734 5_1:+22405829 Msa0724140:CDS
TTAGCCATCTTAGAAGAAGG+AGG 0.666938 5_1:-22407041 None:intergenic
ATGCATAACCGGCATCGGCG+GGG 0.667435 5_1:-22405625 None:intergenic
GGCGGTTCTGGTTATGACGG+CGG 0.676927 5_1:-22405921 None:intergenic
CAAAGAGACTGGATCTCCGG+CGG 0.680570 5_1:-22405945 None:intergenic
GCGGGGTTGGAATCTTGTGG+AGG 0.686429 5_1:-22405608 None:intergenic
TCTCGCACGTGCTCACAGTG+CGG 0.694033 5_1:+22405401 Msa0724140:CDS
GATTGGTAGAGAGTAATTGG+GGG 0.694797 5_1:+22407876 Msa0724140:three_prime_UTR
TATCCATCTTCACATTATGG+CGG 0.696782 5_1:+22406918 Msa0724140:CDS
TCAATTTCAGCAAGAAACAG+TGG 0.701868 5_1:+22406866 Msa0724140:CDS
ATAATGTGAAGATGGATATG+CGG 0.713042 5_1:-22406913 None:intergenic
GTGAAGATGGATATGCGGCG+GGG 0.716036 5_1:-22406908 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTAATGTTATTTGTTT+TGG + chr5_1:22405744-22405763 Msa0724140:intron 10.0%
!! GAAATTGTTAAAAATATAAA+AGG - chr5_1:22406027-22406046 None:intergenic 10.0%
!! TAAAATCAAATTATTAGAAA+AGG - chr5_1:22406049-22406068 None:intergenic 10.0%
!! AATTATACGAAATATATTTA+AGG - chr5_1:22406103-22406122 None:intergenic 10.0%
!!! TTTATGCTTTTTTAATTTAA+TGG + chr5_1:22407770-22407789 Msa0724140:three_prime_UTR 10.0%
!!! AACCTTTTTTAATTAAAATT+AGG + chr5_1:22407821-22407840 Msa0724140:three_prime_UTR 10.0%
!! TAATATATCATGATTACAAT+TGG - chr5_1:22406355-22406374 None:intergenic 15.0%
!!! GTTTTCTATTTTTTTTTCTT+GGG - chr5_1:22407343-22407362 None:intergenic 15.0%
!! TCTCTACTTAATTAATTTAT+TGG - chr5_1:22407365-22407384 None:intergenic 15.0%
!! AATAAATTAATTAAGTAGAG+AGG + chr5_1:22407364-22407383 Msa0724140:three_prime_UTR 15.0%
!! TTAAATTGAGAAAGATAAAA+TGG + chr5_1:22407571-22407590 Msa0724140:three_prime_UTR 15.0%
!! TAAATTGAGAAAGATAAAAT+GGG + chr5_1:22407572-22407591 Msa0724140:three_prime_UTR 15.0%
!!! ATATTCTATTCTATTTTGTA+TGG + chr5_1:22407600-22407619 Msa0724140:three_prime_UTR 15.0%
!! CTAAATTTCAAATATTAGTT+TGG + chr5_1:22407731-22407750 Msa0724140:three_prime_UTR 15.0%
!!! CTCCTAATTTTAATTAAAAA+AGG - chr5_1:22407826-22407845 None:intergenic 15.0%
!!! TGTTTTTATTCTTCTAATAA+AGG + chr5_1:22407909-22407928 Msa0724140:three_prime_UTR 15.0%
!! TGTATAAATAAATCAAGTAA+TGG - chr5_1:22408008-22408027 None:intergenic 15.0%
!! ATTTGAAAAAAAAGAGAGAA+GGG - chr5_1:22405275-22405294 None:intergenic 20.0%
!!! TTATTTCGTTTTACTATAAG+AGG + chr5_1:22406591-22406610 Msa0724140:intron 20.0%
!!! GGTTTTCTATTTTTTTTTCT+TGG - chr5_1:22407344-22407363 None:intergenic 20.0%
!! GAGGATAATATAATATAAGT+AGG + chr5_1:22407383-22407402 Msa0724140:three_prime_UTR 20.0%
!! GATAATATAATATAAGTAGG+AGG + chr5_1:22407386-22407405 Msa0724140:three_prime_UTR 20.0%
!! GGTTAATATGATTAAGAATT+TGG + chr5_1:22407463-22407482 Msa0724140:three_prime_UTR 20.0%
!! TGATTAAGAATTTGGAAAAA+AGG + chr5_1:22407471-22407490 Msa0724140:three_prime_UTR 20.0%
!!! AATTTTGAGAAATAGTAGAT+TGG - chr5_1:22407946-22407965 None:intergenic 20.0%
!!! TAAATAAATCAAGTAATGGA+TGG - chr5_1:22408004-22408023 None:intergenic 20.0%
!! TGATTTATTTATACACTTGA+TGG + chr5_1:22408013-22408032 Msa0724140:three_prime_UTR 20.0%
!! TCTGTAAGAAAAATATATGA+CGG - chr5_1:22408098-22408117 None:intergenic 20.0%
!!! ATTTTTTTTTCCAGTTAAGT+AGG + chr5_1:22408156-22408175 Msa0724140:three_prime_UTR 20.0%
! GATTTGAAAAAAAAGAGAGA+AGG - chr5_1:22405276-22405295 None:intergenic 25.0%
!!! GAAAAAAGCATCATGATTTT+TGG + chr5_1:22405467-22405486 Msa0724140:CDS 25.0%
!!! TTTTCTGAAAGGATTGTTAT+TGG - chr5_1:22405549-22405568 None:intergenic 25.0%
!!! TTATTTGTTTTGGTTGATGA+AGG + chr5_1:22405754-22405773 Msa0724140:intron 25.0%
! AAGAACAAATATTACCGTAT+CGG - chr5_1:22405980-22405999 None:intergenic 25.0%
! ATGAGCTTATGTTAATTGAT+AGG - chr5_1:22406627-22406646 None:intergenic 25.0%
! TATCAATTAACATAAGCTCA+TGG + chr5_1:22406626-22406645 Msa0724140:intron 25.0%
!! TCATTAATGTAATTTTGCCT+TGG + chr5_1:22406797-22406816 Msa0724140:intron 25.0%
!!! TGCCTTGGATTATTTATTTT+AGG + chr5_1:22406812-22406831 Msa0724140:intron 25.0%
! CACCTAAAATAAATAATCCA+AGG - chr5_1:22406817-22406836 None:intergenic 25.0%
!!! GGATTATTTATTTTAGGTGA+TGG + chr5_1:22406818-22406837 Msa0724140:intron 25.0%
!! TGTTGCTTGAAAATTTAGTT+TGG + chr5_1:22407262-22407281 Msa0724140:exon 25.0%
! TTGTAAATTGAGGTTAAAGT+TGG + chr5_1:22407310-22407329 Msa0724140:three_prime_UTR 25.0%
! ATGAATCATGAGAATGATTA+TGG + chr5_1:22407442-22407461 Msa0724140:three_prime_UTR 25.0%
! AAAAGGAAGAAGATAATGTT+AGG + chr5_1:22407488-22407507 Msa0724140:three_prime_UTR 25.0%
! AAGATAATGTTAGGTTATGT+AGG + chr5_1:22407497-22407516 Msa0724140:three_prime_UTR 25.0%
!!! ATTCTTGTTTCTATTTGTCT+GGG + chr5_1:22407633-22407652 Msa0724140:three_prime_UTR 25.0%
! TATTTCTCAAAATTCTCTCT+TGG + chr5_1:22407952-22407971 Msa0724140:three_prime_UTR 25.0%
! CTGGAAAAAAAAATACTTGA+TGG - chr5_1:22408150-22408169 None:intergenic 25.0%
! AGAACTTAAGATAAATGAGT+TGG - chr5_1:22408237-22408256 None:intergenic 25.0%
! TTCTAATAATATGAGAGAGT+TGG - chr5_1:22408417-22408436 None:intergenic 25.0%
TCTAGATTATGTTCCTTACA+TGG + chr5_1:22405318-22405337 Msa0724140:five_prime_UTR 30.0%
TTGTTCATATTGAGAAAGGT+TGG - chr5_1:22405585-22405604 None:intergenic 30.0%
!! CAAACAAAAGTTGATGTATC+TGG - chr5_1:22405712-22405731 None:intergenic 30.0%
CTAGAAACTTTGTGAAAACT+AGG + chr5_1:22405857-22405876 Msa0724140:CDS 30.0%
TGTCCGGAGATAATATATTT+GGG - chr5_1:22406685-22406704 None:intergenic 30.0%
ATGTCCGGAGATAATATATT+TGG - chr5_1:22406686-22406705 None:intergenic 30.0%
TCATCTTCCATTATAGTTGA+AGG - chr5_1:22406846-22406865 None:intergenic 30.0%
ATAATGTGAAGATGGATATG+CGG - chr5_1:22406916-22406935 None:intergenic 30.0%
!!! AGTTTGGTCAAGTTTTTTCT+TGG + chr5_1:22407278-22407297 Msa0724140:three_prime_UTR 30.0%
ATAATATAAGTAGGAGGACT+AGG + chr5_1:22407392-22407411 Msa0724140:three_prime_UTR 30.0%
TAATATAAGTAGGAGGACTA+GGG + chr5_1:22407393-22407412 Msa0724140:three_prime_UTR 30.0%
CCAAAAACCACCAATTAATT+CGG - chr5_1:22407530-22407549 None:intergenic 30.0%
TTGAGAAAGATAAAATGGGT+TGG + chr5_1:22407576-22407595 Msa0724140:three_prime_UTR 30.0%
! ATTCTATTTTGTATGGCTGT+TGG + chr5_1:22407607-22407626 Msa0724140:three_prime_UTR 30.0%
! TTCTATTTTGTATGGCTGTT+GGG + chr5_1:22407608-22407627 Msa0724140:three_prime_UTR 30.0%
!! GATTCTTGTTTCTATTTGTC+TGG + chr5_1:22407632-22407651 Msa0724140:three_prime_UTR 30.0%
!! TTCTTGTTTCTATTTGTCTG+GGG + chr5_1:22407634-22407653 Msa0724140:three_prime_UTR 30.0%
! CAAATATTAGTTTGGACAAG+AGG + chr5_1:22407739-22407758 Msa0724140:three_prime_UTR 30.0%
!!! GATTTTTTCCGTACATAGAT+TGG + chr5_1:22407859-22407878 Msa0724140:three_prime_UTR 30.0%
ATAGATTGGTAGAGAGTAAT+TGG + chr5_1:22407873-22407892 Msa0724140:three_prime_UTR 30.0%
TAGATTGGTAGAGAGTAATT+GGG + chr5_1:22407874-22407893 Msa0724140:three_prime_UTR 30.0%
!! TAAATCAAGTAATGGATGGA+AGG - chr5_1:22408000-22408019 None:intergenic 30.0%
AGTCAGATGACTAGAAATAA+GGG - chr5_1:22408287-22408306 None:intergenic 30.0%
ATGAGAGAGAACAAGATAAT+TGG + chr5_1:22408440-22408459 Msa0724140:three_prime_UTR 30.0%
AGAACAAGATAATTGGAAGA+AGG + chr5_1:22408447-22408466 Msa0724140:three_prime_UTR 30.0%
!!! AGAAGGCAAAGTTTTAGTTA+AGG + chr5_1:22408464-22408483 Msa0724140:three_prime_UTR 30.0%
! AGAGATGATTTGCGAGATTT+GGG - chr5_1:22405206-22405225 None:intergenic 35.0%
TGAAAAAAAAGAGAGAAGGG+TGG - chr5_1:22405272-22405291 None:intergenic 35.0%
TGCTGAAATCAATCCATGTA+AGG - chr5_1:22405334-22405353 None:intergenic 35.0%
! TCTGAAAGGATTGTTATTGG+TGG - chr5_1:22405546-22405565 None:intergenic 35.0%
!!! ATGCTAGCACTTTTTCTGAA+AGG - chr5_1:22405560-22405579 None:intergenic 35.0%
GTGGTTGTTCATATTGAGAA+AGG - chr5_1:22405589-22405608 None:intergenic 35.0%
CTTTGATATTACCACCGATA+CGG + chr5_1:22405963-22405982 Msa0724140:CDS 35.0%
AACAAATATTACCGTATCGG+TGG - chr5_1:22405977-22405996 None:intergenic 35.0%
! GATAATATATTTGGGACGGA+GGG - chr5_1:22406677-22406696 None:intergenic 35.0%
! AGATAATATATTTGGGACGG+AGG - chr5_1:22406678-22406697 None:intergenic 35.0%
CGGAGATAATATATTTGGGA+CGG - chr5_1:22406681-22406700 None:intergenic 35.0%
CGTCCCAAATATATTATCTC+CGG + chr5_1:22406679-22406698 Msa0724140:intron 35.0%
CGTTCGCAAAAATATATGTC+CGG - chr5_1:22406701-22406720 None:intergenic 35.0%
GATGGAACCTTCAACTATAA+TGG + chr5_1:22406836-22406855 Msa0724140:CDS 35.0%
TCAATTTCAGCAAGAAACAG+TGG + chr5_1:22406866-22406885 Msa0724140:CDS 35.0%
GCATATCCATCTTCACATTA+TGG + chr5_1:22406915-22406934 Msa0724140:CDS 35.0%
TATCCATCTTCACATTATGG+CGG + chr5_1:22406918-22406937 Msa0724140:CDS 35.0%
! AGATTAGCCATCTTAGAAGA+AGG - chr5_1:22407047-22407066 None:intergenic 35.0%
AGCTTCAATGACAATGGAAA+AGG - chr5_1:22407110-22407129 None:intergenic 35.0%
!!! ATCATCGCCTTTTAAAACCA+TGG + chr5_1:22407196-22407215 Msa0724140:CDS 35.0%
!!! CAAGTTTTTTCTTGGTTGAC+TGG + chr5_1:22407286-22407305 Msa0724140:three_prime_UTR 35.0%
!! GTTGACTGGTTTGTAAATTG+AGG + chr5_1:22407300-22407319 Msa0724140:three_prime_UTR 35.0%
! AGGTGATTAGCCGAATTAAT+TGG + chr5_1:22407517-22407536 Msa0724140:three_prime_UTR 35.0%
! TGATTAGCCGAATTAATTGG+TGG + chr5_1:22407520-22407539 Msa0724140:three_prime_UTR 35.0%
!! CCGAATTAATTGGTGGTTTT+TGG + chr5_1:22407527-22407546 Msa0724140:three_prime_UTR 35.0%
!!! TGGTTTTTGGTGTACATGTT+TGG + chr5_1:22407540-22407559 Msa0724140:three_prime_UTR 35.0%
! TCTATTTTGTATGGCTGTTG+GGG + chr5_1:22407609-22407628 Msa0724140:three_prime_UTR 35.0%
!! TCTTGTTTCTATTTGTCTGG+GGG + chr5_1:22407635-22407654 Msa0724140:three_prime_UTR 35.0%
! TTGGTCTATTTCTTGCAACT+TGG + chr5_1:22407704-22407723 Msa0724140:three_prime_UTR 35.0%
ACTCTCTACCAATCTATGTA+CGG - chr5_1:22407870-22407889 None:intergenic 35.0%
AGATTGGTAGAGAGTAATTG+GGG + chr5_1:22407875-22407894 Msa0724140:three_prime_UTR 35.0%
! TCACTTTCTTTTGACGAACA+TGG + chr5_1:22408041-22408060 Msa0724140:three_prime_UTR 35.0%
GAGTCAGATGACTAGAAATA+AGG - chr5_1:22408288-22408307 None:intergenic 35.0%
GATAGAAACTAGACCTATGT+GGG - chr5_1:22408370-22408389 None:intergenic 35.0%
TGATAGAAACTAGACCTATG+TGG - chr5_1:22408371-22408390 None:intergenic 35.0%
ATTTGCGAGATTTGGGTGAT+AGG - chr5_1:22405199-22405218 None:intergenic 40.0%
! GAGAGATGATTTGCGAGATT+TGG - chr5_1:22405207-22405226 None:intergenic 40.0%
!!! ATTTTTGGTGTTCGACTCAC+CGG + chr5_1:22405482-22405501 Msa0724140:CDS 40.0%
! GAAAGGATTGTTATTGGTGG+TGG - chr5_1:22405543-22405562 None:intergenic 40.0%
AGAACACAAGTTGTTCTTGC+TGG + chr5_1:22405795-22405814 Msa0724140:CDS 40.0%
GAACACAAGTTGTTCTTGCT+GGG + chr5_1:22405796-22405815 Msa0724140:CDS 40.0%
AACACAAGTTGTTCTTGCTG+GGG + chr5_1:22405797-22405816 Msa0724140:CDS 40.0%
!! AACAGGTTGGTAAAGGTGAT+TGG + chr5_1:22405824-22405843 Msa0724140:CDS 40.0%
TATCAAAGAGACTGGATCTC+CGG - chr5_1:22405951-22405970 None:intergenic 40.0%
GGTGGTAATATCAAAGAGAC+TGG - chr5_1:22405959-22405978 None:intergenic 40.0%
ACTCTTTCAGCACTCATTAC+CGG - chr5_1:22406990-22407009 None:intergenic 40.0%
TTAGCCATCTTAGAAGAAGG+AGG - chr5_1:22407044-22407063 None:intergenic 40.0%
!!! CTTTTAAAACCATGGCTGCA+GGG + chr5_1:22407204-22407223 Msa0724140:CDS 40.0%
! CTATTTTGTATGGCTGTTGG+GGG + chr5_1:22407610-22407629 Msa0724140:three_prime_UTR 40.0%
AGTGTGACCTTGAAGTGATA+GGG + chr5_1:22407658-22407677 Msa0724140:three_prime_UTR 40.0%
GATTGGTAGAGAGTAATTGG+GGG + chr5_1:22407876-22407895 Msa0724140:three_prime_UTR 40.0%
!! AAGGTCACATTTTGAGGGTT+GGG - chr5_1:22407981-22408000 None:intergenic 40.0%
! GATGGAAGGTCACATTTTGA+GGG - chr5_1:22407986-22408005 None:intergenic 40.0%
ACTGTAACGTCGTTTGATTC+AGG - chr5_1:22408190-22408209 None:intergenic 40.0%
TTACATACCCAAACCCACAT+AGG + chr5_1:22408354-22408373 Msa0724140:three_prime_UTR 40.0%
AACTAGACCTATGTGGGTTT+GGG - chr5_1:22408364-22408383 None:intergenic 40.0%
AAACTAGACCTATGTGGGTT+TGG - chr5_1:22408365-22408384 None:intergenic 40.0%
AACTCGAGAACATGCAACGA+CGG + chr5_1:22405437-22405456 Msa0724140:CDS 45.0%
!! ATTGTTATTGGTGGTGGTGG+TGG - chr5_1:22405537-22405556 None:intergenic 45.0%
!! AGGATTGTTATTGGTGGTGG+TGG - chr5_1:22405540-22405559 None:intergenic 45.0%
GATGTCATCGGATGCATAAC+CGG - chr5_1:22405639-22405658 None:intergenic 45.0%
!! TGGTTGATGAAGGTGTTCCT+TGG + chr5_1:22405764-22405783 Msa0724140:intron 45.0%
TTGTGTTCTTCCTCTGTCCA+AGG - chr5_1:22405784-22405803 None:intergenic 45.0%
! GTTGGTAAAGGTGATTGGAG+AGG + chr5_1:22405829-22405848 Msa0724140:CDS 45.0%
GAAAACTAGGACACCAACTC+AGG + chr5_1:22405870-22405889 Msa0724140:CDS 45.0%
ATGTGAAGATGGATATGCGG+CGG - chr5_1:22406913-22406932 None:intergenic 45.0%
ATGCCGCCATAATGTGAAGA+TGG - chr5_1:22406924-22406943 None:intergenic 45.0%
CTCATTACCGGCAATGTTAC+CGG - chr5_1:22406978-22406997 None:intergenic 45.0%
!! GGTAATGAGTGCTGAAAGAG+TGG + chr5_1:22406989-22407008 Msa0724140:CDS 45.0%
AAAGAGTGGCCAAGCCAATT+AGG + chr5_1:22407003-22407022 Msa0724140:CDS 45.0%
TTAGAAGAAGGAGGCAGCAT+TGG - chr5_1:22407035-22407054 None:intergenic 45.0%
TGACAATGGAAAAGGCTCGA+TGG - chr5_1:22407102-22407121 None:intergenic 45.0%
GGTTGCAGCTTCAATGACAA+TGG - chr5_1:22407116-22407135 None:intergenic 45.0%
GTCATTGAAGCTGCAACCTT+CGG + chr5_1:22407118-22407137 Msa0724140:CDS 45.0%
TCCATCGGAAGATCATTCAC+CGG + chr5_1:22407142-22407161 Msa0724140:CDS 45.0%
!!! CCTGCAGCCATGGTTTTAAA+AGG - chr5_1:22407206-22407225 None:intergenic 45.0%
!!! CCTTTTAAAACCATGGCTGC+AGG + chr5_1:22407203-22407222 Msa0724140:CDS 45.0%
ATTATACTTCCCTGCAGCCA+TGG - chr5_1:22407216-22407235 None:intergenic 45.0%
! ATGGCTGCAGGGAAGTATAA+TGG + chr5_1:22407215-22407234 Msa0724140:CDS 45.0%
! CTGCAGGGAAGTATAATGGT+GGG + chr5_1:22407219-22407238 Msa0724140:CDS 45.0%
! TGCAGGGAAGTATAATGGTG+GGG + chr5_1:22407220-22407239 Msa0724140:CDS 45.0%
TATAAGTAGGAGGACTAGGG+AGG + chr5_1:22407396-22407415 Msa0724140:three_prime_UTR 45.0%
ATAAGTAGGAGGACTAGGGA+GGG + chr5_1:22407397-22407416 Msa0724140:three_prime_UTR 45.0%
GAGTGTGACCTTGAAGTGAT+AGG + chr5_1:22407657-22407676 Msa0724140:three_prime_UTR 45.0%
ACCTTGAAGTGATAGGGCAA+TGG + chr5_1:22407664-22407683 Msa0724140:three_prime_UTR 45.0%
CCTTGAAGTGATAGGGCAAT+GGG + chr5_1:22407665-22407684 Msa0724140:three_prime_UTR 45.0%
! GGGTACTGTTGGTCTCTTAT+TGG + chr5_1:22407685-22407704 Msa0724140:three_prime_UTR 45.0%
!! GAAGGTCACATTTTGAGGGT+TGG - chr5_1:22407982-22408001 None:intergenic 45.0%
! GGATGGAAGGTCACATTTTG+AGG - chr5_1:22407987-22408006 None:intergenic 45.0%
! CGTTGCATGTTCTCGAGTTG+TGG - chr5_1:22405436-22405455 None:intergenic 50.0%
ATCGGATGCATAACCGGCAT+CGG - chr5_1:22405633-22405652 None:intergenic 50.0%
! AGAGGCGTGAACGATGTCAT+CGG - chr5_1:22405651-22405670 None:intergenic 50.0%
!! TGAAGGTGTTCCTTGGACAG+AGG + chr5_1:22405771-22405790 Msa0724140:intron 50.0%
!! GGGTTGCAACAGGTTGGTAA+AGG + chr5_1:22405817-22405836 Msa0724140:CDS 50.0%
! GCATGACTTGCAACCTGAGT+TGG - chr5_1:22405886-22405905 None:intergenic 50.0%
TGTGAAGATGGATATGCGGC+GGG - chr5_1:22406912-22406931 None:intergenic 50.0%
! ACACCTTTTCCGATGGGTCT+TGG + chr5_1:22406951-22406970 Msa0724140:CDS 50.0%
! TTTTCCGATGGGTCTTGGTC+CGG + chr5_1:22406956-22406975 Msa0724140:CDS 50.0%
TCTTGGTCCGGTAACATTGC+CGG + chr5_1:22406968-22406987 Msa0724140:CDS 50.0%
!! AGCATTGGTGTCGGCCTAAT+TGG - chr5_1:22407020-22407039 None:intergenic 50.0%
! GCTGCCTCCTTCTTCTAAGA+TGG + chr5_1:22407037-22407056 Msa0724140:CDS 50.0%
TGATCTTCCGATGGAACCGA+AGG - chr5_1:22407137-22407156 None:intergenic 50.0%
TCCGGTGAATGATCTTCCGA+TGG - chr5_1:22407146-22407165 None:intergenic 50.0%
!! GACGAATGGCTGCTACTTTC+CGG - chr5_1:22407164-22407183 None:intergenic 50.0%
TAGCAGCCATTCGTCGTCAT+CGG + chr5_1:22407169-22407188 Msa0724140:CDS 50.0%
ATGAAGCCGATGACGACGAA+TGG - chr5_1:22407178-22407197 None:intergenic 50.0%
! GCTGCAGGGAAGTATAATGG+TGG + chr5_1:22407218-22407237 Msa0724140:CDS 50.0%
! GCAGGGAAGTATAATGGTGG+GGG + chr5_1:22407221-22407240 Msa0724140:CDS 50.0%
! GGAAGTATAATGGTGGGGGT+GGG + chr5_1:22407225-22407244 Msa0724140:CDS 50.0%
! GAAGTATAATGGTGGGGGTG+GGG + chr5_1:22407226-22407245 Msa0724140:CDS 50.0%
CCCATTGCCCTATCACTTCA+AGG - chr5_1:22407668-22407687 None:intergenic 50.0%
! GATAGGGCAATGGGTACTGT+TGG + chr5_1:22407674-22407693 Msa0724140:three_prime_UTR 50.0%
TGGTAGAGAGTAATTGGGGG+AGG + chr5_1:22407879-22407898 Msa0724140:three_prime_UTR 50.0%
TAGAGAGTAATTGGGGGAGG+TGG + chr5_1:22407882-22407901 Msa0724140:three_prime_UTR 50.0%
GGCAGTCGATCCTACTTAAC+TGG - chr5_1:22408169-22408188 None:intergenic 50.0%
TGCTCACAGTGCGGCAACAA+CGG + chr5_1:22405410-22405429 Msa0724140:CDS 55.0%
TCGAGAACATGCAACGACGG+TGG + chr5_1:22405440-22405459 Msa0724140:CDS 55.0%
!! TTTGGTGTTCGACTCACCGG+AGG + chr5_1:22405485-22405504 Msa0724140:CDS 55.0%
! GTTGGGGTGGTTGTTTCCTC+CGG - chr5_1:22405504-22405523 None:intergenic 55.0%
!! GTTATTGGTGGTGGTGGTGG+TGG - chr5_1:22405534-22405553 None:intergenic 55.0%
GGGTTGGAATCTTGTGGAGG+TGG - chr5_1:22405608-22405627 None:intergenic 55.0%
GATGACATCGTTCACGCCTC+TGG + chr5_1:22405650-22405669 Msa0724140:CDS 55.0%
CGATCCAGAGAACGCAAACG+AGG + chr5_1:22405674-22405693 Msa0724140:CDS 55.0%
CTGACCTCGTTTGCGTTCTC+TGG - chr5_1:22405681-22405700 None:intergenic 55.0%
GTTCTTGCTGGGGTTGCAAC+AGG + chr5_1:22405807-22405826 Msa0724140:CDS 55.0%
TTGCTGGGGTTGCAACAGGT+TGG + chr5_1:22405811-22405830 Msa0724140:CDS 55.0%
CAAAGAGACTGGATCTCCGG+CGG - chr5_1:22405948-22405967 None:intergenic 55.0%
TGAAGATGGATATGCGGCGG+GGG - chr5_1:22406910-22406929 None:intergenic 55.0%
GTGAAGATGGATATGCGGCG+GGG - chr5_1:22406911-22406930 None:intergenic 55.0%
TCACATTATGGCGGCATCCC+TGG + chr5_1:22406927-22406946 Msa0724140:CDS 55.0%
CACATTATGGCGGCATCCCT+GGG + chr5_1:22406928-22406947 Msa0724140:CDS 55.0%
!! CCATCGGAAAAGGTGTCCCA+GGG - chr5_1:22406947-22406966 None:intergenic 55.0%
! CCTGGGACACCTTTTCCGAT+GGG + chr5_1:22406945-22406964 Msa0724140:CDS 55.0%
GGACCAAGACCCATCGGAAA+AGG - chr5_1:22406957-22406976 None:intergenic 55.0%
GTTACCGGACCAAGACCCAT+CGG - chr5_1:22406963-22406982 None:intergenic 55.0%
!! TGGTGTCGGCCTAATTGGCT+TGG - chr5_1:22407015-22407034 None:intergenic 55.0%
!! GAAGGAGGCAGCATTGGTGT+CGG - chr5_1:22407029-22407048 None:intergenic 55.0%
GCTGCAACCTTCGGTTCCAT+CGG + chr5_1:22407127-22407146 Msa0724140:CDS 55.0%
! GGGAAGTATAATGGTGGGGG+TGG + chr5_1:22407224-22407243 Msa0724140:CDS 55.0%
TCTCGCACGTGCTCACAGTG+CGG + chr5_1:22405401-22405420 Msa0724140:CDS 60.0%
!! TGGTGGTGGTGGAGGTGTTG+GGG - chr5_1:22405520-22405539 None:intergenic 60.0%
!! GTGGTGGTGGTGGAGGTGTT+GGG - chr5_1:22405521-22405540 None:intergenic 60.0%
!! ATTGGTGGTGGTGGTGGTGG+TGG - chr5_1:22405531-22405550 None:intergenic 60.0%
GCGGGGTTGGAATCTTGTGG+AGG - chr5_1:22405611-22405630 None:intergenic 60.0%
TCGGCGGGGTTGGAATCTTG+TGG - chr5_1:22405614-22405633 None:intergenic 60.0%
!! ATGCATAACCGGCATCGGCG+GGG - chr5_1:22405628-22405647 None:intergenic 60.0%
!! GATGCATAACCGGCATCGGC+GGG - chr5_1:22405629-22405648 None:intergenic 60.0%
! GGATGCATAACCGGCATCGG+CGG - chr5_1:22405630-22405649 None:intergenic 60.0%
GCGTTCTCTGGATCGACCAG+AGG - chr5_1:22405669-22405688 None:intergenic 60.0%
! GGTTCTGGTTATGACGGCGG+AGG - chr5_1:22405921-22405940 None:intergenic 60.0%
! GGCGGTTCTGGTTATGACGG+CGG - chr5_1:22405924-22405943 None:intergenic 60.0%
! GGCGGCGGTTCTGGTTATGA+CGG - chr5_1:22405927-22405946 None:intergenic 60.0%
! CCCTGGGACACCTTTTCCGA+TGG + chr5_1:22406944-22406963 Msa0724140:CDS 60.0%
!! CCCATCGGAAAAGGTGTCCC+AGG - chr5_1:22406948-22406967 None:intergenic 60.0%
GGACTAGGGAGGGACTAGAG+AGG + chr5_1:22407407-22407426 Msa0724140:three_prime_UTR 60.0%
!! TGGTGGTGGAGGTGTTGGGG+TGG - chr5_1:22405517-22405536 None:intergenic 65.0%
!! GGTGGTGGTGGTGGAGGTGT+TGG - chr5_1:22405522-22405541 None:intergenic 65.0%
GATTCCAACCCCGCCGATGC+CGG + chr5_1:22405617-22405636 Msa0724140:CDS 65.0%
!! ATAACCGGCATCGGCGGGGT+TGG - chr5_1:22405624-22405643 None:intergenic 65.0%
ATAACCAGAACCGCCGCCGC+CGG + chr5_1:22405929-22405948 Msa0724140:CDS 65.0%
AGAGACTGGATCTCCGGCGG+CGG - chr5_1:22405945-22405964 None:intergenic 65.0%
GATGGATATGCGGCGGGGGT+TGG - chr5_1:22406906-22406925 None:intergenic 65.0%
!! GGTGGTGGTGGTGGTGGTGG+AGG - chr5_1:22405528-22405547 None:intergenic 70.0%
!! TATGCGGCGGGGGTTGGTGG+TGG - chr5_1:22406900-22406919 None:intergenic 70.0%
GGATATGCGGCGGGGGTTGG+TGG - chr5_1:22406903-22406922 None:intergenic 70.0%
ATCTCCGGCGGCGGCGGTTC+TGG - chr5_1:22405936-22405955 None:intergenic 75.0%
GACTGGATCTCCGGCGGCGG+CGG - chr5_1:22405942-22405961 None:intergenic 75.0%
!! GCGGGGGTTGGTGGTGGCAG+TGG - chr5_1:22406894-22406913 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 22405176 22408491 22405176 ID=Msa0724140;Name=Msa0724140
chr5_1 mRNA 22405176 22408491 22405176 ID=Msa0724140-mRNA-1;Parent=Msa0724140;Name=Msa0724140-mRNA-1;_AED=0.01;_eAED=0.01;_QI=216|1|1|1|1|1|3|1220|316
chr5_1 exon 22405176 22405695 22405176 ID=Msa0724140-mRNA-1:exon:8450;Parent=Msa0724140-mRNA-1
chr5_1 exon 22405776 22405984 22405776 ID=Msa0724140-mRNA-1:exon:8451;Parent=Msa0724140-mRNA-1
chr5_1 exon 22406834 22408491 22406834 ID=Msa0724140-mRNA-1:exon:8452;Parent=Msa0724140-mRNA-1
chr5_1 five_prime_UTR 22405176 22405391 22405176 ID=Msa0724140-mRNA-1:five_prime_utr;Parent=Msa0724140-mRNA-1
chr5_1 CDS 22405392 22405695 22405392 ID=Msa0724140-mRNA-1:cds;Parent=Msa0724140-mRNA-1
chr5_1 CDS 22405776 22405984 22405776 ID=Msa0724140-mRNA-1:cds;Parent=Msa0724140-mRNA-1
chr5_1 CDS 22406834 22407271 22406834 ID=Msa0724140-mRNA-1:cds;Parent=Msa0724140-mRNA-1
chr5_1 three_prime_UTR 22407272 22408491 22407272 ID=Msa0724140-mRNA-1:three_prime_utr;Parent=Msa0724140-mRNA-1
Gene Sequence

>Msa0724140

ATGTCTTTGTCTCGCACGTGCTCACAGTGCGGCAACAACGGCCACAACTCGAGAACATGCAACGACGGTGGAGAAGAAAAAAGCATCATGATTTTTGGTGTTCGACTCACCGGAGGAAACAACCACCCCAACACCTCCACCACCACCACCACCACCAATAACAATCCTTTCAGAAAAAGTGCTAGCATGACCAACCTTTCTCAATATGAACAACCACCTCCACAAGATTCCAACCCCGCCGATGCCGGTTATGCATCCGATGACATCGTTCACGCCTCTGGTCGATCCAGAGAACGCAAACGAGGTGTTCCTTGGACAGAGGAAGAACACAAGTTGTTCTTGCTGGGGTTGCAACAGGTTGGTAAAGGTGATTGGAGAGGAATTTCTAGAAACTTTGTGAAAACTAGGACACCAACTCAGGTTGCAAGTCATGCTCAGAAGTATTTCCTCCGCCGTCATAACCAGAACCGCCGCCGCCGGAGATCCAGTCTCTTTGATATTACCACCGATACGGTGATGGAACCTTCAACTATAATGGAAGATGATCAATTTCAGCAAGAAACAGTGGCGCCACTGCCACCACCAACCCCCGCCGCATATCCATCTTCACATTATGGCGGCATCCCTGGGACACCTTTTCCGATGGGTCTTGGTCCGGTAACATTGCCGGTAATGAGTGCTGAAAGAGTGGCCAAGCCAATTAGGCCGACACCAATGCTGCCTCCTTCTTCTAAGATGGCTAATCTGAACTTGAAAGACAAAGCTGCTTCTTCTTCTTCCATCGAGCCTTTTCCATTGTCATTGAAGCTGCAACCTTCGGTTCCATCGGAAGATCATTCACCGGAAAGTAGCAGCCATTCGTCGTCATCGGCTTCATCATCGCCTTTTAAAACCATGGCTGCAGGGAAGTATAATGGTGGGGGTGGGGATAGCATTATCAGTGTTGCTTGA
Protein sequence

>Msa0724140

MSLSRTCSQCGNNGHNSRTCNDGGEEKSIMIFGVRLTGGNNHPNTSTTTTTTNNNPFRKSASMTNLSQYEQPPPQDSNPADAGYASDDIVHASGRSRERKRGVPWTEEEHKLFLLGLQQVGKGDWRGISRNFVKTRTPTQVASHAQKYFLRRHNQNRRRRRSSLFDITTDTVMEPSTIMEDDQFQQETVAPLPPPTPAAYPSSHYGGIPGTPFPMGLGPVTLPVMSAERVAKPIRPTPMLPPSSKMANLNLKDKAASSSSIEPFPLSLKLQPSVPSEDHSPESSSHSSSSASSSPFKTMAAGKYNGGGGDSIISVA*