AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686210 MtrunA17_Chr4g0035001 98.795 83 1 0 1 83 1 83 1.34e-57 177
Msa0686210 MtrunA17_Chr4g0035051 90.244 82 8 0 2 83 4 85 1.90e-52 162
Msa0686210 MtrunA17_Chr4g0035031 84.337 83 13 0 2 84 4 86 1.06e-48 155
Msa0686210 MtrunA17_Chr2g0278661 57.955 88 36 1 1 87 1 88 1.75e-33 118
Msa0686210 MtrunA17_Chr4g0050781 55.814 86 37 1 1 85 1 86 9.86e-31 110
Msa0686210 MtrunA17_Chr3g0116631 54.321 81 37 0 6 86 206 286 4.44e-27 102
Msa0686210 MtrunA17_Chr3g0116631 33.000 100 67 0 7 106 110 209 2.85e-12 63.2
Msa0686210 MtrunA17_Chr4g0061451 52.500 80 38 0 7 86 43 122 2.53e-25 97.8
Msa0686210 MtrunA17_Chr4g0058151 53.086 81 38 0 6 86 216 296 1.01e-24 97.1
Msa0686210 MtrunA17_Chr4g0058151 35.577 104 64 1 5 108 121 221 1.01e-14 70.1
Msa0686210 MtrunA17_Chr1g0210241 49.383 81 41 0 5 85 6 86 4.24e-23 92.0
Msa0686210 MtrunA17_Chr3g0086861 50.633 79 39 0 7 85 45 123 5.03e-23 94.0
Msa0686210 MtrunA17_Chr1g0181111 51.250 80 39 0 7 86 197 276 3.84e-21 87.0
Msa0686210 MtrunA17_Chr7g0219021 53.782 119 55 0 7 125 41 159 1.45e-20 85.1
Msa0686210 MtrunA17_Chr3g0119311 42.683 82 47 0 7 88 37 118 2.23e-19 79.0
Msa0686210 MtrunA17_Chr7g0266751 39.583 96 56 1 4 97 30 125 8.10e-19 77.8
Msa0686210 MtrunA17_Chr1g0168601 40.244 82 49 0 2 83 149 230 8.42e-18 77.4
Msa0686210 MtrunA17_Chr3g0121631 48.760 121 51 2 7 127 37 146 1.88e-17 74.3
Msa0686210 MtrunA17_Chr4g0017401 40.000 115 58 5 3 108 12 124 1.09e-16 74.7
Msa0686210 MtrunA17_Chr3g0127751 32.117 137 79 3 7 132 27 160 1.37e-16 74.3
Msa0686210 MtrunA17_Chr7g0219031 45.455 66 36 0 7 72 41 106 2.19e-16 70.9
Msa0686210 MtrunA17_Chr3g0101061 37.975 79 47 1 7 85 41 117 1.40e-15 73.2
Msa0686210 MtrunA17_Chr7g0276531 50.847 59 29 0 7 65 19 77 6.31e-15 70.1
Msa0686210 MtrunA17_Chr2g0290191 37.975 79 47 1 7 85 5 81 7.03e-15 70.9
Msa0686210 MtrunA17_Chr3g0144311 40.741 81 46 2 3 82 25 104 7.55e-15 70.1
Msa0686210 MtrunA17_Chr1g0186351 32.911 79 51 1 7 85 7 83 1.32e-14 70.1
Msa0686210 MtrunA17_Chr1g0186351 32.955 88 58 1 7 94 113 199 7.21e-13 65.1
Msa0686210 MtrunA17_Chr7g0263001 39.241 79 48 0 7 85 36 114 1.52e-14 66.6
Msa0686210 MtrunA17_Chr5g0447761 36.709 79 48 1 7 85 7 83 1.66e-14 70.1
Msa0686210 MtrunA17_Chr5g0447761 32.258 93 62 1 7 99 107 198 5.78e-12 62.8
Msa0686210 MtrunA17_Chr6g0481071 37.500 88 55 0 7 94 96 183 2.10e-14 67.4
Msa0686210 MtrunA17_Chr4g0018581 46.667 75 40 0 8 82 453 527 2.33e-14 69.7
Msa0686210 MtrunA17_Chr5g0426311 43.421 76 42 1 7 82 17 91 2.53e-14 68.6
Msa0686210 MtrunA17_Chr3g0120721 34.328 134 83 3 7 139 107 236 2.75e-14 68.9
Msa0686210 MtrunA17_Chr3g0120721 31.068 103 69 1 7 109 19 119 1.92e-13 66.6
Msa0686210 MtrunA17_Chr6g0449481 35.417 96 60 1 7 102 46 139 2.93e-14 68.9
Msa0686210 MtrunA17_Chr6g0449481 33.333 132 74 2 7 124 134 265 3.33e-11 60.5
Msa0686210 MtrunA17_Chr2g0327921 36.585 82 52 0 7 88 8 89 3.57e-14 64.3
Msa0686210 MtrunA17_Chr4g0042161 36.275 102 59 2 1 99 46 144 6.85e-14 65.1
Msa0686210 MtrunA17_Chr8g0346011 42.308 78 40 2 7 82 38 112 8.07e-14 67.4
Msa0686210 MtrunA17_Chr3g0110211 35.514 107 68 1 8 113 114 220 3.74e-13 65.9
Msa0686210 MtrunA17_Chr4g0062931 35.256 156 91 5 8 158 9 159 4.19e-13 65.9
Msa0686210 MtrunA17_Chr3g0088511 43.243 74 42 0 7 80 33 106 6.47e-13 62.4
Msa0686210 MtrunA17_Chr4g0031091 38.356 73 45 0 7 79 34 106 9.62e-13 61.2
Msa0686210 MtrunA17_Chr5g0402191 40.000 80 44 2 7 85 5 81 1.17e-12 64.7
Msa0686210 MtrunA17_Chr3g0135111 35.714 98 58 2 7 101 11 106 2.17e-12 63.5
Msa0686210 MtrunA17_Chr3g0135111 27.778 126 89 2 7 132 118 241 8.66e-11 59.3
Msa0686210 MtrunA17_Chr4g0065741 37.662 77 48 0 7 83 72 148 3.77e-12 60.8
Msa0686210 MtrunA17_Chr7g0267571 36.667 90 57 0 2 91 208 297 6.96e-12 62.0
Msa0686210 MtrunA17_Chr4g0050441 38.462 78 48 0 6 83 16 93 1.26e-11 61.2
Msa0686210 MtrunA17_Chr5g0429941 38.554 83 50 1 1 83 12 93 1.27e-11 60.8
Msa0686210 MtrunA17_Chr8g0360551 37.209 86 48 3 7 90 74 155 5.39e-11 59.7
Msa0686210 MtrunA17_Chr5g0419351 34.343 99 65 0 8 106 12 110 8.50e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686210 AT4G39260.3 74.725 91 23 0 1 91 1 91 3.02e-48 151
Msa0686210 AT2G21660.2 82.716 81 14 0 3 83 5 85 3.97e-46 148
Msa0686210 AT2G21660.1 82.716 81 14 0 3 83 5 85 6.64e-46 148
Msa0686210 AT4G39260.2 72.289 83 23 0 1 83 1 83 5.99e-43 139
Msa0686210 AT4G39260.1 72.289 83 23 0 1 83 1 83 2.19e-42 139
Msa0686210 AT4G39260.4 72.059 68 19 0 1 68 1 68 3.08e-34 116
Msa0686210 AT3G26420.1 35.321 218 80 4 3 159 4 221 8.15e-29 106
Msa0686210 AT5G61030.2 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0686210 AT5G61030.1 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0686210 AT3G23830.2 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0686210 AT3G23830.1 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0686210 AT5G50250.1 51.807 83 40 0 3 85 204 286 6.30e-25 97.4
Msa0686210 AT5G50250.1 40.000 90 54 0 5 94 112 201 1.01e-14 70.1
Msa0686210 AT4G13850.4 53.086 81 38 0 7 87 36 116 1.14e-24 92.8
Msa0686210 AT4G24770.1 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0686210 AT4G24770.1 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0686210 AT4G24770.2 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0686210 AT4G24770.2 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0686210 AT4G13850.1 52.525 99 47 0 7 105 36 134 3.75e-23 89.7
Msa0686210 AT5G04280.1 47.312 93 46 1 7 96 8 100 4.68e-23 93.2
Msa0686210 AT2G21690.1 49.438 89 40 1 2 85 3 91 9.31e-22 85.1
Msa0686210 AT2G37220.1 45.679 81 44 0 7 87 205 285 3.90e-21 87.4
Msa0686210 AT1G74230.1 57.647 85 35 1 7 91 35 118 4.56e-21 87.4
Msa0686210 AT3G53460.1 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0686210 AT3G53460.3 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0686210 AT3G53460.2 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0686210 AT3G53460.4 45.783 83 45 0 7 89 279 361 7.04e-21 87.8
Msa0686210 AT5G06210.1 45.556 90 49 0 4 93 32 121 1.07e-20 83.2
Msa0686210 AT1G60650.2 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0686210 AT1G60650.1 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0686210 AT4G13850.2 46.552 116 49 1 7 122 36 138 3.21e-19 79.7
Msa0686210 AT4G13850.3 44.628 121 45 1 7 127 36 134 2.15e-18 77.0
Msa0686210 AT5G47320.1 46.753 77 41 0 7 83 32 108 2.56e-17 75.9
Msa0686210 AT1G18630.1 52.083 96 46 0 7 102 37 132 2.77e-17 74.7
Msa0686210 AT1G22330.1 43.137 102 52 3 7 103 18 118 4.79e-17 76.6
Msa0686210 AT2G46780.1 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0686210 AT2G46780.2 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0686210 AT4G13860.1 44.595 74 41 0 7 80 4 77 2.19e-16 70.5
Msa0686210 AT4G14300.1 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0686210 AT4G14300.1 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0686210 AT4G14300.2 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0686210 AT4G14300.2 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0686210 AT3G13224.3 38.710 93 51 2 1 89 1 91 4.35e-16 74.3
Msa0686210 AT3G13224.3 32.323 99 63 1 7 105 101 195 1.87e-14 70.1
Msa0686210 AT3G07810.2 33.028 109 71 1 7 115 7 113 8.47e-16 73.9
Msa0686210 AT3G07810.1 33.028 109 71 1 7 115 7 113 9.69e-16 73.9
Msa0686210 AT2G37510.1 45.946 74 40 0 7 80 35 108 1.04e-15 70.1
Msa0686210 AT5G59865.1 44.304 79 44 0 9 87 29 107 1.33e-15 68.9
Msa0686210 AT3G13224.2 39.759 83 48 1 7 89 20 100 1.44e-15 73.2
Msa0686210 AT3G13224.2 34.568 81 53 0 7 87 110 190 1.12e-14 70.5
Msa0686210 AT1G78260.1 44.444 81 43 2 3 82 13 92 1.65e-15 72.4
Msa0686210 AT1G76460.1 44.737 76 41 1 7 82 25 99 2.12e-15 72.0
Msa0686210 AT3G08000.1 44.048 84 47 0 7 90 42 125 2.64e-15 69.3
Msa0686210 AT4G26650.1 39.241 79 46 1 7 85 16 92 2.78e-15 72.4
Msa0686210 AT4G26650.2 39.241 79 46 1 7 85 13 89 2.97e-15 72.4
Msa0686210 AT1G78260.2 44.444 81 43 2 3 82 13 92 3.03e-15 71.2
Msa0686210 AT1G22910.1 36.364 99 56 3 3 94 9 107 4.12e-15 70.9
Msa0686210 AT1G20880.2 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0686210 AT1G20880.3 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0686210 AT1G20880.1 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0686210 AT2G33410.1 32.558 86 56 1 7 92 7 90 5.83e-15 71.6
Msa0686210 AT2G33410.1 37.975 79 48 1 7 85 111 188 3.68e-12 63.5
Msa0686210 AT1G22910.2 36.364 99 56 3 3 94 9 107 6.11e-15 70.1
Msa0686210 AT5G55550.5 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0686210 AT5G55550.4 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0686210 AT5G55550.9 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0686210 AT5G55550.7 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0686210 AT5G55550.1 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0686210 AT1G60000.1 40.244 82 49 0 2 83 173 254 7.05e-15 70.1
Msa0686210 AT5G55550.2 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0686210 AT5G55550.3 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0686210 AT5G55550.6 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0686210 AT5G55550.11 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0686210 AT1G33470.3 37.500 104 58 3 1 98 1 103 1.53e-14 68.9
Msa0686210 AT1G33470.1 37.500 104 58 3 1 98 1 103 1.79e-14 68.9
Msa0686210 AT1G33470.2 37.500 104 58 3 1 98 1 103 1.80e-14 68.9
Msa0686210 AT5G19960.1 43.038 79 44 1 8 86 9 86 2.42e-14 69.7
Msa0686210 AT1G22910.3 36.364 99 56 3 3 94 9 107 4.40e-14 68.9
Msa0686210 AT3G13224.1 39.286 84 49 1 7 90 20 101 1.09e-13 66.6
Msa0686210 AT5G55550.8 42.254 71 39 1 7 77 7 75 1.88e-13 67.4
Msa0686210 AT5G51300.1 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0686210 AT5G51300.2 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0686210 AT5G51300.3 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0686210 AT5G55550.10 42.254 71 39 1 7 77 7 75 2.38e-13 67.0
Msa0686210 AT3G54770.3 42.105 76 43 1 7 82 18 92 2.76e-13 65.9
Msa0686210 AT3G46020.1 37.662 77 48 0 7 83 8 84 2.99e-13 62.8
Msa0686210 AT3G54770.1 42.105 76 43 1 7 82 18 92 2.99e-13 65.9
Msa0686210 AT5G47620.1 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0686210 AT5G47620.2 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0686210 AT5G47620.5 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0686210 AT5G40490.1 36.458 96 59 1 7 102 43 136 3.80e-13 66.2
Msa0686210 AT5G40490.1 32.292 96 63 1 7 100 131 226 1.14e-11 62.0
Msa0686210 AT1G17640.2 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0686210 AT1G17640.2 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0686210 AT1G17640.1 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0686210 AT1G17640.1 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0686210 AT1G17640.3 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0686210 AT1G17640.3 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0686210 AT3G52150.2 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0686210 AT3G52150.1 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0686210 AT2G27330.1 31.579 76 52 0 9 84 24 99 6.86e-13 62.0
Msa0686210 AT1G73530.1 31.818 88 60 0 7 94 78 165 9.92e-13 63.2
Msa0686210 AT1G01080.1 37.349 83 47 2 1 81 207 286 1.61e-12 63.9
Msa0686210 AT5G53720.1 41.250 80 46 1 3 82 6 84 1.63e-12 60.8
Msa0686210 AT1G01080.2 37.349 83 47 2 1 81 208 287 1.74e-12 63.9
Msa0686210 AT5G64200.2 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0686210 AT5G64200.1 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0686210 AT3G14100.1 39.744 78 47 0 5 82 143 220 4.53e-12 63.2
Msa0686210 AT5G47620.4 36.709 79 44 2 7 81 7 83 7.13e-12 62.8
Msa0686210 AT4G13860.2 45.763 59 32 0 22 80 2 60 1.12e-11 57.8
Msa0686210 AT1G54080.1 39.744 78 47 0 5 82 147 224 2.05e-11 61.2
Msa0686210 AT5G11412.1 42.169 83 44 3 2 82 47 127 2.56e-11 59.3
Msa0686210 AT4G36960.1 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0686210 AT4G36960.2 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0686210 AT5G04280.3 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0686210 AT5G04280.4 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0686210 AT5G04280.5 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0686210 AT5G04280.2 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0686210 AT5G40490.2 35.052 97 59 3 7 100 114 209 4.46e-11 60.5
Msa0686210 AT2G18510.1 30.081 123 85 1 8 129 114 236 5.95e-11 60.1
Msa0686210 AT5G54580.1 41.026 78 46 0 8 85 58 135 6.31e-11 57.8
Msa0686210 AT1G01080.3 40.000 55 33 0 1 55 207 261 9.93e-11 58.9

Find 89 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAGGTGATGGTGGTTATT+AGG 0.231697 4_4:+50532389 Msa0686210:CDS
GGAGGTGGTGGTGGTGGATA+TGG 0.236629 4_4:+50532216 Msa0686210:CDS
GGTGGAAGCGGTGGTGGATA+TGG 0.268521 4_4:+50532258 Msa0686210:CDS
GGTGGAGGAGGTGGTGGATA+TGG 0.274998 4_4:+50532279 Msa0686210:CDS
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4_4:+50532045 Msa0686210:CDS
ATCATTAACGATCGTGAAAC+TGG 0.286078 4_4:+50532027 Msa0686210:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.292757 4_4:+50532195 Msa0686210:CDS
CACTTAAAACACAGATAATA+TGG 0.313021 4_4:-50532587 None:intergenic
AGCAATGAACGGTCAGGATT+TGG 0.313573 4_4:+50532113 Msa0686210:CDS
TGCTTCAATGGCATCGTTCA+TGG 0.319166 4_4:-50532094 None:intergenic
GGAGGCGGTGGTGGAGGATA+TGG 0.330951 4_4:+50532345 Msa0686210:CDS
GGAGGAGGTGGTGGATATGG+TGG 0.343759 4_4:+50532282 Msa0686210:CDS
GGTGGATATGGAGGTGGTGG+TGG 0.355416 4_4:+50532207 Msa0686210:CDS
GGTGGATATGGTGGAGGAGG+TGG 0.362609 4_4:+50532270 Msa0686210:CDS
ATGATTGTTATTCTAGATGT+TGG 0.374897 4_4:+50532431 Msa0686210:three_prime_UTR
GGTGGAGAGCGTCGTGGATA+TGG 0.378333 4_4:+50532237 Msa0686210:CDS
CTGACCGTTCATTGCTTCAA+TGG 0.378604 4_4:-50532106 None:intergenic
GGCGGAGGAGGCCGTGGTGG+CGG 0.382681 4_4:+50532186 Msa0686210:CDS
GGATATGGTGGAGGAGGTGG+TGG 0.390425 4_4:+50532273 Msa0686210:CDS
GGCGGTGGTGGAGGATATGG+TGG 0.396144 4_4:+50532348 Msa0686210:CDS
TACAGCAGAGGAGGCGGTGG+TGG 0.397231 4_4:+50532336 Msa0686210:CDS
GGATATGGAGGTGGTGGTGG+TGG 0.398226 4_4:+50532210 Msa0686210:CDS
CGCTACCACGGCTCTGAGCT+TGG 0.401331 4_4:-50532158 None:intergenic
TGATTGTTATTCTAGATGTT+GGG 0.408580 4_4:+50532432 Msa0686210:three_prime_UTR
GGCGGTGGATATGGAGGTGG+TGG 0.414719 4_4:+50532204 Msa0686210:CDS
GGTGGTGACCGCCGTGGTGG+AGG 0.417792 4_4:+50532306 Msa0686210:CDS
GGTGGTGGTGGTGGATATGG+TGG 0.419545 4_4:+50532219 Msa0686210:CDS
GGAGGTGGTGGATATGGTGG+TGG 0.423953 4_4:+50532285 Msa0686210:CDS
GGTAGCGGTGGTGGCGGAGG+AGG 0.428094 4_4:+50532174 Msa0686210:CDS
GACCGCCGTGGTGGAGGTGG+TGG 0.428431 4_4:+50532312 Msa0686210:CDS
CGTGGTAGCGGTGGTGGCGG+AGG 0.433194 4_4:+50532171 Msa0686210:CDS
CAGAGCCGTGGTAGCGGTGG+TGG 0.439087 4_4:+50532165 Msa0686210:CDS
GGTGGTGGCGGAGGAGGCCG+TGG 0.439963 4_4:+50532180 Msa0686210:CDS
GGTGACCGCCGTGGTGGAGG+TGG 0.440014 4_4:+50532309 Msa0686210:CDS
AGCAGAGGAGGCGGTGGTGG+AGG 0.451847 4_4:+50532339 Msa0686210:CDS
GGATATGGTGGAAGCGGTGG+TGG 0.454383 4_4:+50532252 Msa0686210:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.459076 4_4:+50532189 Msa0686210:CDS
AGCCGTGGTAGCGGTGGTGG+CGG 0.473368 4_4:+50532168 Msa0686210:CDS
GGAAGCGGTGGTGGATATGG+TGG 0.474255 4_4:+50532261 Msa0686210:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.475042 4_4:+50531608 Msa0686210:CDS
GGTGGTGGAGGATATGGTGG+TGG 0.479646 4_4:+50532351 Msa0686210:CDS
GGTGGCGGAGGAGGCCGTGG+TGG 0.482575 4_4:+50532183 Msa0686210:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.485578 4_4:+50532288 Msa0686210:CDS
ACAAAACACAGGTAATACTT+TGG 0.498137 4_4:-50532660 None:intergenic
GAGAGAGGTTATAGAGGCAA+AGG 0.499481 4_4:-50531524 None:intergenic
GGTGGAGGATATGGTGGTGG+TGG 0.501369 4_4:+50532354 Msa0686210:CDS
GGATACAGCAGAGGAGGCGG+TGG 0.503411 4_4:+50532333 Msa0686210:CDS
CAGCCATTGAAAAGAGCAAA+GGG 0.505087 4_4:-50531570 None:intergenic
GGTGGATACAGCAGAGGAGG+CGG 0.513743 4_4:+50532330 Msa0686210:CDS
CATTGAAGCAATGAACGGTC+AGG 0.516837 4_4:+50532107 Msa0686210:CDS
CAAGACCTCCGACGAAGCAT+CGG 0.524569 4_4:-50531603 None:intergenic
GTTGAATACCGATGCTTCGT+CGG 0.525860 4_4:+50531595 Msa0686210:CDS
TACAGCAGAGGAGGAGGTGA+TGG 0.532656 4_4:+50532378 Msa0686210:CDS
CAAGCTCAGAGCCGTGGTAG+CGG 0.533686 4_4:+50532159 Msa0686210:CDS
CGGCGAGATCGTTGATTCCA+AGG 0.539852 4_4:+50531672 Msa0686210:CDS
AGCGGTGGTGGATATGGTGG+AGG 0.541831 4_4:+50532264 Msa0686210:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.543855 4_4:+50531652 Msa0686210:CDS
TTTCTCGAGAGCATCGTTGT+CGG 0.548634 4_4:-50531635 None:intergenic
CGTGAAACTGGACGATCAAG+AGG 0.551662 4_4:+50532039 Msa0686210:CDS
CGTGGTGGCGGTGGATATGG+AGG 0.555369 4_4:+50532198 Msa0686210:CDS
GGTGGATACAGCAGAGGAGG+AGG 0.557224 4_4:+50532372 Msa0686210:CDS
GGTGGTGGATATGGTGGAGG+AGG 0.560670 4_4:+50532267 Msa0686210:CDS
CGTCGTGGATATGGTGGAAG+CGG 0.562578 4_4:+50532246 Msa0686210:CDS
GGTGGTGGATACAGCAGAGG+AGG 0.562608 4_4:+50532327 Msa0686210:CDS
CGTGGATATGGTGGAAGCGG+TGG 0.562637 4_4:+50532249 Msa0686210:CDS
GGTGGTGGTGACCGCCGTGG+TGG 0.562802 4_4:+50532303 Msa0686210:CDS
CTCCATATCCACCGCCACCA+CGG 0.564840 4_4:-50532197 None:intergenic
GCTCAGAGCCGTGGTAGCGG+TGG 0.565666 4_4:+50532162 Msa0686210:CDS
GGTGGCGGTGGATATGGAGG+TGG 0.573835 4_4:+50532201 Msa0686210:CDS
GGTGGTGGATACAGCAGAGG+AGG 0.582601 4_4:+50532369 Msa0686210:CDS
CTCGAGAGCATCGTTGTCGG+TGG 0.585909 4_4:-50531632 None:intergenic
GAATACCGATGCTTCGTCGG+AGG 0.590495 4_4:+50531598 Msa0686210:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.591814 4_4:+50531609 Msa0686210:CDS
CTCCGCCACCACCGCTACCA+CGG 0.595147 4_4:-50532170 None:intergenic
AGCAGAGGAGGAGGTGATGG+TGG 0.597479 4_4:+50532381 Msa0686210:CDS
TCAGCCATTGAAAAGAGCAA+AGG 0.600296 4_4:-50531571 None:intergenic
ATGAACGGTCAGGATTTGGA+TGG 0.621464 4_4:+50532117 Msa0686210:CDS
GATCGTTATACACAAAACAC+AGG 0.633135 4_4:-50532671 None:intergenic
GGAGAGCGTCGTGGATATGG+TGG 0.640307 4_4:+50532240 Msa0686210:CDS
GTGAACCAAGCTCAGAGCCG+TGG 0.643330 4_4:+50532153 Msa0686210:CDS
GATCTCGCCGTATTGAGAGA+AGG 0.650761 4_4:-50531659 None:intergenic
GGTGGTGGTGGTGACCGCCG+TGG 0.665791 4_4:+50532300 Msa0686210:CDS
GGAGGTGGTGGATACAGCAG+AGG 0.669900 4_4:+50532324 Msa0686210:CDS
GGTGGTGGTGGATACAGCAG+AGG 0.670896 4_4:+50532366 Msa0686210:CDS
GATGCCATTGAAGCAATGAA+CGG 0.670960 4_4:+50532102 Msa0686210:CDS
AAAACTGAATTAAGGTTTGG+GGG 0.698108 4_4:-50531546 None:intergenic
TGTATCCACCACCTCCACCA+CGG 0.715403 4_4:-50532317 None:intergenic
GGATATGGTGGAGAGCGTCG+TGG 0.717480 4_4:+50532231 Msa0686210:CDS
ATCCACCACCTCCACCACGG+CGG 0.754503 4_4:-50532314 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_4:50532640-50532659 None:intergenic 10.0%
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_4:50532640-50532659 None:intergenic 10.0%
!!! TTTTTTTTCTTTTGATGTTT+TGG + chr4_4:50531967-50531986 Msa0686210:intron 15.0%
!!! TTTTTTTCTTTTGATGTTTT+GGG + chr4_4:50531968-50531987 Msa0686210:intron 15.0%
!!! TTTTTTTTCTTTTGATGTTT+TGG + chr4_4:50531967-50531986 Msa0686210:intron 15.0%
!!! TTTTTTTCTTTTGATGTTTT+GGG + chr4_4:50531968-50531987 Msa0686210:intron 15.0%
! AAAAACTGAATTAAGGTTTG+GGG - chr4_4:50531550-50531569 None:intergenic 25.0%
! GAAAAACTGAATTAAGGTTT+GGG - chr4_4:50531551-50531570 None:intergenic 25.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_4:50532431-50532450 Msa0686210:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_4:50532432-50532451 Msa0686210:three_prime_UTR 25.0%
! CACTTAAAACACAGATAATA+TGG - chr4_4:50532590-50532609 None:intergenic 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_4:50532636-50532655 None:intergenic 25.0%
! AAAAACTGAATTAAGGTTTG+GGG - chr4_4:50531550-50531569 None:intergenic 25.0%
! GAAAAACTGAATTAAGGTTT+GGG - chr4_4:50531551-50531570 None:intergenic 25.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_4:50532431-50532450 Msa0686210:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_4:50532432-50532451 Msa0686210:three_prime_UTR 25.0%
! CACTTAAAACACAGATAATA+TGG - chr4_4:50532590-50532609 None:intergenic 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_4:50532636-50532655 None:intergenic 25.0%
AAAACTGAATTAAGGTTTGG+GGG - chr4_4:50531549-50531568 None:intergenic 30.0%
GGAAAAACTGAATTAAGGTT+TGG - chr4_4:50531552-50531571 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+AGG - chr4_4:50531557-50531576 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_4:50531734-50531753 Msa0686210:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_4:50531910-50531929 Msa0686210:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_4:50531984-50532003 Msa0686210:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_4:50532474-50532493 Msa0686210:three_prime_UTR 30.0%
ACAAAACACAGGTAATACTT+TGG - chr4_4:50532663-50532682 None:intergenic 30.0%
AAAACTGAATTAAGGTTTGG+GGG - chr4_4:50531549-50531568 None:intergenic 30.0%
GGAAAAACTGAATTAAGGTT+TGG - chr4_4:50531552-50531571 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+AGG - chr4_4:50531557-50531576 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_4:50531734-50531753 Msa0686210:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_4:50531910-50531929 Msa0686210:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_4:50531984-50532003 Msa0686210:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_4:50532474-50532493 Msa0686210:three_prime_UTR 30.0%
ACAAAACACAGGTAATACTT+TGG - chr4_4:50532663-50532682 None:intergenic 30.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_4:50531567-50531586 Msa0686210:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_4:50531724-50531743 Msa0686210:intron 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_4:50531866-50531885 Msa0686210:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_4:50531884-50531903 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_4:50531921-50531940 Msa0686210:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_4:50531935-50531954 Msa0686210:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_4:50532027-50532046 Msa0686210:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_4:50532500-50532519 Msa0686210:three_prime_UTR 35.0%
GATCGTTATACACAAAACAC+AGG - chr4_4:50532674-50532693 None:intergenic 35.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_4:50531567-50531586 Msa0686210:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_4:50531724-50531743 Msa0686210:intron 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_4:50531866-50531885 Msa0686210:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_4:50531884-50531903 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_4:50531921-50531940 Msa0686210:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_4:50531935-50531954 Msa0686210:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_4:50532027-50532046 Msa0686210:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_4:50532500-50532519 Msa0686210:three_prime_UTR 35.0%
GATCGTTATACACAAAACAC+AGG - chr4_4:50532674-50532693 None:intergenic 35.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_4:50531573-50531592 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_4:50531574-50531593 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_4:50531652-50531671 Msa0686210:CDS 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_4:50531782-50531801 None:intergenic 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_4:50532102-50532121 Msa0686210:CDS 40.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_4:50531573-50531592 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_4:50531574-50531593 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_4:50531652-50531671 Msa0686210:CDS 40.0%
CAGAGTAACACAGAACAGAT+CGG - chr4_4:50531782-50531801 None:intergenic 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_4:50532102-50532121 Msa0686210:CDS 40.0%
GAGAGAGGTTATAGAGGCAA+AGG - chr4_4:50531527-50531546 None:intergenic 45.0%
AATTAAGGTTTGGGGGAGAG+AGG - chr4_4:50531542-50531561 None:intergenic 45.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_4:50531595-50531614 Msa0686210:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_4:50531638-50531657 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_4:50531763-50531782 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_4:50532045-50532064 Msa0686210:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_4:50532097-50532116 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_4:50532109-50532128 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_4:50532107-50532126 Msa0686210:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_4:50532113-50532132 Msa0686210:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_4:50532117-50532136 Msa0686210:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_4:50532389-50532408 Msa0686210:CDS 45.0%
GAGAGAGGTTATAGAGGCAA+AGG - chr4_4:50531527-50531546 None:intergenic 45.0%
AATTAAGGTTTGGGGGAGAG+AGG - chr4_4:50531542-50531561 None:intergenic 45.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_4:50531595-50531614 Msa0686210:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_4:50531638-50531657 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_4:50531763-50531782 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_4:50532045-50532064 Msa0686210:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_4:50532097-50532116 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_4:50532109-50532128 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_4:50532107-50532126 Msa0686210:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_4:50532113-50532132 Msa0686210:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_4:50532117-50532136 Msa0686210:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_4:50532389-50532408 Msa0686210:CDS 45.0%
TTGGGGGAGAGAGGTTATAG+AGG - chr4_4:50531533-50531552 None:intergenic 50.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_4:50531662-50531681 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_4:50531692-50531711 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_4:50531691-50531710 Msa0686210:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_4:50531699-50531718 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_4:50531697-50531716 Msa0686210:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_4:50532039-50532058 Msa0686210:CDS 50.0%
TTGGGGGAGAGAGGTTATAG+AGG - chr4_4:50531533-50531552 None:intergenic 50.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_4:50531662-50531681 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_4:50531692-50531711 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_4:50531691-50531710 Msa0686210:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_4:50531699-50531718 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_4:50531697-50531716 Msa0686210:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_4:50532039-50532058 Msa0686210:CDS 50.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_4:50531598-50531617 Msa0686210:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_4:50531606-50531625 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_4:50531609-50531628 Msa0686210:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_4:50531672-50531691 Msa0686210:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_4:50531702-50531721 Msa0686210:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_4:50532246-50532265 Msa0686210:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_4:50532320-50532339 None:intergenic 55.0%
TACAGCAGAGGAGGAGGTGA+TGG + chr4_4:50532378-50532397 Msa0686210:CDS 55.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_4:50531598-50531617 Msa0686210:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_4:50531606-50531625 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_4:50531609-50531628 Msa0686210:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_4:50531672-50531691 Msa0686210:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_4:50531702-50531721 Msa0686210:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_4:50532246-50532265 Msa0686210:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_4:50532320-50532339 None:intergenic 55.0%
TACAGCAGAGGAGGAGGTGA+TGG + chr4_4:50532378-50532397 Msa0686210:CDS 55.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_4:50531608-50531627 Msa0686210:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_4:50531635-50531654 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_4:50532153-50532172 Msa0686210:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_4:50532159-50532178 Msa0686210:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_4:50532200-50532219 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_4:50532207-50532226 Msa0686210:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_4:50532210-50532229 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_4:50532216-50532235 Msa0686210:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_4:50532219-50532238 Msa0686210:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_4:50532231-50532250 Msa0686210:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_4:50532237-50532256 Msa0686210:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_4:50532240-50532259 Msa0686210:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_4:50532249-50532268 Msa0686210:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_4:50532252-50532271 Msa0686210:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_4:50532258-50532277 Msa0686210:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_4:50532261-50532280 Msa0686210:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_4:50532264-50532283 Msa0686210:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_4:50532267-50532286 Msa0686210:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_4:50532270-50532289 Msa0686210:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_4:50532273-50532292 Msa0686210:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_4:50532279-50532298 Msa0686210:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_4:50532282-50532301 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_4:50532285-50532304 Msa0686210:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_4:50532288-50532307 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_4:50532324-50532343 Msa0686210:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_4:50532327-50532346 Msa0686210:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_4:50532330-50532349 Msa0686210:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_4:50532351-50532370 Msa0686210:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_4:50532354-50532373 Msa0686210:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+AGG + chr4_4:50532366-50532385 Msa0686210:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_4:50532327-50532346 Msa0686210:CDS 60.0%
GGTGGATACAGCAGAGGAGG+AGG + chr4_4:50532330-50532349 Msa0686210:CDS 60.0%
! AGCAGAGGAGGAGGTGATGG+TGG + chr4_4:50532381-50532400 Msa0686210:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_4:50531608-50531627 Msa0686210:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_4:50531635-50531654 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_4:50532153-50532172 Msa0686210:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_4:50532159-50532178 Msa0686210:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_4:50532200-50532219 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_4:50532207-50532226 Msa0686210:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_4:50532210-50532229 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_4:50532216-50532235 Msa0686210:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_4:50532219-50532238 Msa0686210:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_4:50532231-50532250 Msa0686210:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_4:50532237-50532256 Msa0686210:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_4:50532240-50532259 Msa0686210:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_4:50532249-50532268 Msa0686210:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_4:50532252-50532271 Msa0686210:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_4:50532258-50532277 Msa0686210:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_4:50532261-50532280 Msa0686210:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_4:50532264-50532283 Msa0686210:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_4:50532267-50532286 Msa0686210:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_4:50532270-50532289 Msa0686210:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_4:50532273-50532292 Msa0686210:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_4:50532279-50532298 Msa0686210:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_4:50532282-50532301 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_4:50532285-50532304 Msa0686210:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_4:50532288-50532307 Msa0686210:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_4:50532324-50532343 Msa0686210:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_4:50532327-50532346 Msa0686210:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_4:50532330-50532349 Msa0686210:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_4:50532351-50532370 Msa0686210:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_4:50532354-50532373 Msa0686210:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+AGG + chr4_4:50532366-50532385 Msa0686210:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_4:50532327-50532346 Msa0686210:CDS 60.0%
GGTGGATACAGCAGAGGAGG+AGG + chr4_4:50532330-50532349 Msa0686210:CDS 60.0%
! AGCAGAGGAGGAGGTGATGG+TGG + chr4_4:50532381-50532400 Msa0686210:CDS 60.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_4:50532161-50532180 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_4:50532198-50532217 Msa0686210:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_4:50532201-50532220 Msa0686210:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_4:50532204-50532223 Msa0686210:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_4:50532317-50532336 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_4:50532333-50532352 Msa0686210:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_4:50532336-50532355 Msa0686210:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_4:50532345-50532364 Msa0686210:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_4:50532348-50532367 Msa0686210:CDS 65.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_4:50532161-50532180 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_4:50532198-50532217 Msa0686210:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_4:50532201-50532220 Msa0686210:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_4:50532204-50532223 Msa0686210:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_4:50532317-50532336 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_4:50532333-50532352 Msa0686210:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_4:50532336-50532355 Msa0686210:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_4:50532345-50532364 Msa0686210:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_4:50532348-50532367 Msa0686210:CDS 65.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_4:50532162-50532181 Msa0686210:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_4:50532165-50532184 Msa0686210:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_4:50532168-50532187 Msa0686210:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_4:50532173-50532192 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_4:50532195-50532214 Msa0686210:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_4:50532339-50532358 Msa0686210:CDS 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_4:50532162-50532181 Msa0686210:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_4:50532165-50532184 Msa0686210:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_4:50532168-50532187 Msa0686210:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_4:50532173-50532192 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_4:50532195-50532214 Msa0686210:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_4:50532339-50532358 Msa0686210:CDS 70.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_4:50532171-50532190 Msa0686210:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_4:50532174-50532193 Msa0686210:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_4:50532300-50532319 Msa0686210:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_4:50532303-50532322 Msa0686210:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_4:50532306-50532325 Msa0686210:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_4:50532309-50532328 Msa0686210:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_4:50532312-50532331 Msa0686210:CDS 75.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_4:50532171-50532190 Msa0686210:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_4:50532174-50532193 Msa0686210:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_4:50532300-50532319 Msa0686210:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_4:50532303-50532322 Msa0686210:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_4:50532306-50532325 Msa0686210:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_4:50532309-50532328 Msa0686210:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_4:50532312-50532331 Msa0686210:CDS 75.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_4:50532180-50532199 Msa0686210:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_4:50532183-50532202 Msa0686210:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_4:50532186-50532205 Msa0686210:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_4:50532189-50532208 Msa0686210:CDS 80.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_4:50532180-50532199 Msa0686210:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_4:50532183-50532202 Msa0686210:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_4:50532186-50532205 Msa0686210:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_4:50532189-50532208 Msa0686210:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50531509 50532713 50531509 ID=Msa0686210;Name=Msa0686210
chr4_4 mRNA 50531509 50532713 50531509 ID=Msa0686210-mRNA-1;Parent=Msa0686210;Name=Msa0686210-mRNA-1;_AED=0.09;_eAED=0.09;_QI=77|1|1|1|1|1|2|303|163
chr4_4 exon 50531509 50531693 50531509 ID=Msa0686210-mRNA-1:exon:12946;Parent=Msa0686210-mRNA-1
chr4_4 exon 50532027 50532713 50532027 ID=Msa0686210-mRNA-1:exon:12947;Parent=Msa0686210-mRNA-1
chr4_4 five_prime_UTR 50531509 50531585 50531509 ID=Msa0686210-mRNA-1:five_prime_utr;Parent=Msa0686210-mRNA-1
chr4_4 CDS 50531586 50531693 50531586 ID=Msa0686210-mRNA-1:cds;Parent=Msa0686210-mRNA-1
chr4_4 CDS 50532027 50532410 50532027 ID=Msa0686210-mRNA-1:cds;Parent=Msa0686210-mRNA-1
chr4_4 three_prime_UTR 50532411 50532713 50532411 ID=Msa0686210-mRNA-1:three_prime_utr;Parent=Msa0686210-mRNA-1
Gene Sequence

>Msa0686210

ATGGCTGATGTTGAATACCGATGCTTCGTCGGAGGTCTTGCATGGGCCACCGACAACGATGCTCTCGAGAAAGCCTTCTCTCAATACGGCGAGATCGTTGATTCCAAGATCATTAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTTGTGACATTCGCTAACGAGAAATCCATGAACGATGCCATTGAAGCAATGAACGGTCAGGATTTGGATGGACGTAACATCACTGTGAACCAAGCTCAGAGCCGTGGTAGCGGTGGTGGCGGAGGAGGCCGTGGTGGCGGTGGATATGGAGGTGGTGGTGGTGGATATGGTGGAGAGCGTCGTGGATATGGTGGAAGCGGTGGTGGATATGGTGGAGGAGGTGGTGGATATGGTGGTGGTGGTGACCGCCGTGGTGGAGGTGGTGGATACAGCAGAGGAGGCGGTGGTGGAGGATATGGTGGTGGTGGATACAGCAGAGGAGGAGGTGATGGTGGTTATTAG
Protein sequence

>Msa0686210

MADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDLDGRNITVNQAQSRGSGGGGGGRGGGGYGGGGGGYGGERRGYGGSGGGYGGGGGGYGGGGDRRGGGGGYSRGGGGGGYGGGGYSRGGGDGGY*