AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550120 MtrunA17_Chr4g0035001 98.795 83 1 0 1 83 1 83 1.34e-57 177
Msa0550120 MtrunA17_Chr4g0035051 90.244 82 8 0 2 83 4 85 1.90e-52 162
Msa0550120 MtrunA17_Chr4g0035031 84.337 83 13 0 2 84 4 86 1.06e-48 155
Msa0550120 MtrunA17_Chr2g0278661 57.955 88 36 1 1 87 1 88 1.75e-33 118
Msa0550120 MtrunA17_Chr4g0050781 55.814 86 37 1 1 85 1 86 9.86e-31 110
Msa0550120 MtrunA17_Chr3g0116631 54.321 81 37 0 6 86 206 286 4.44e-27 102
Msa0550120 MtrunA17_Chr3g0116631 33.000 100 67 0 7 106 110 209 2.85e-12 63.2
Msa0550120 MtrunA17_Chr4g0061451 52.500 80 38 0 7 86 43 122 2.53e-25 97.8
Msa0550120 MtrunA17_Chr4g0058151 53.086 81 38 0 6 86 216 296 1.01e-24 97.1
Msa0550120 MtrunA17_Chr4g0058151 35.577 104 64 1 5 108 121 221 1.01e-14 70.1
Msa0550120 MtrunA17_Chr1g0210241 49.383 81 41 0 5 85 6 86 4.24e-23 92.0
Msa0550120 MtrunA17_Chr3g0086861 50.633 79 39 0 7 85 45 123 5.03e-23 94.0
Msa0550120 MtrunA17_Chr1g0181111 51.250 80 39 0 7 86 197 276 3.84e-21 87.0
Msa0550120 MtrunA17_Chr7g0219021 53.782 119 55 0 7 125 41 159 1.45e-20 85.1
Msa0550120 MtrunA17_Chr3g0119311 42.683 82 47 0 7 88 37 118 2.23e-19 79.0
Msa0550120 MtrunA17_Chr7g0266751 39.583 96 56 1 4 97 30 125 8.10e-19 77.8
Msa0550120 MtrunA17_Chr1g0168601 40.244 82 49 0 2 83 149 230 8.42e-18 77.4
Msa0550120 MtrunA17_Chr3g0121631 48.760 121 51 2 7 127 37 146 1.88e-17 74.3
Msa0550120 MtrunA17_Chr4g0017401 40.000 115 58 5 3 108 12 124 1.09e-16 74.7
Msa0550120 MtrunA17_Chr3g0127751 32.117 137 79 3 7 132 27 160 1.37e-16 74.3
Msa0550120 MtrunA17_Chr7g0219031 45.455 66 36 0 7 72 41 106 2.19e-16 70.9
Msa0550120 MtrunA17_Chr3g0101061 37.975 79 47 1 7 85 41 117 1.40e-15 73.2
Msa0550120 MtrunA17_Chr7g0276531 50.847 59 29 0 7 65 19 77 6.31e-15 70.1
Msa0550120 MtrunA17_Chr2g0290191 37.975 79 47 1 7 85 5 81 7.03e-15 70.9
Msa0550120 MtrunA17_Chr3g0144311 40.741 81 46 2 3 82 25 104 7.55e-15 70.1
Msa0550120 MtrunA17_Chr1g0186351 32.911 79 51 1 7 85 7 83 1.32e-14 70.1
Msa0550120 MtrunA17_Chr1g0186351 32.955 88 58 1 7 94 113 199 7.21e-13 65.1
Msa0550120 MtrunA17_Chr7g0263001 39.241 79 48 0 7 85 36 114 1.52e-14 66.6
Msa0550120 MtrunA17_Chr5g0447761 36.709 79 48 1 7 85 7 83 1.66e-14 70.1
Msa0550120 MtrunA17_Chr5g0447761 32.258 93 62 1 7 99 107 198 5.78e-12 62.8
Msa0550120 MtrunA17_Chr6g0481071 37.500 88 55 0 7 94 96 183 2.10e-14 67.4
Msa0550120 MtrunA17_Chr4g0018581 46.667 75 40 0 8 82 453 527 2.33e-14 69.7
Msa0550120 MtrunA17_Chr5g0426311 43.421 76 42 1 7 82 17 91 2.53e-14 68.6
Msa0550120 MtrunA17_Chr3g0120721 34.328 134 83 3 7 139 107 236 2.75e-14 68.9
Msa0550120 MtrunA17_Chr3g0120721 31.068 103 69 1 7 109 19 119 1.92e-13 66.6
Msa0550120 MtrunA17_Chr6g0449481 35.417 96 60 1 7 102 46 139 2.93e-14 68.9
Msa0550120 MtrunA17_Chr6g0449481 33.333 132 74 2 7 124 134 265 3.33e-11 60.5
Msa0550120 MtrunA17_Chr2g0327921 36.585 82 52 0 7 88 8 89 3.57e-14 64.3
Msa0550120 MtrunA17_Chr4g0042161 36.275 102 59 2 1 99 46 144 6.85e-14 65.1
Msa0550120 MtrunA17_Chr8g0346011 42.308 78 40 2 7 82 38 112 8.07e-14 67.4
Msa0550120 MtrunA17_Chr3g0110211 35.514 107 68 1 8 113 114 220 3.74e-13 65.9
Msa0550120 MtrunA17_Chr4g0062931 35.256 156 91 5 8 158 9 159 4.19e-13 65.9
Msa0550120 MtrunA17_Chr3g0088511 43.243 74 42 0 7 80 33 106 6.47e-13 62.4
Msa0550120 MtrunA17_Chr4g0031091 38.356 73 45 0 7 79 34 106 9.62e-13 61.2
Msa0550120 MtrunA17_Chr5g0402191 40.000 80 44 2 7 85 5 81 1.17e-12 64.7
Msa0550120 MtrunA17_Chr3g0135111 35.714 98 58 2 7 101 11 106 2.17e-12 63.5
Msa0550120 MtrunA17_Chr3g0135111 27.778 126 89 2 7 132 118 241 8.66e-11 59.3
Msa0550120 MtrunA17_Chr4g0065741 37.662 77 48 0 7 83 72 148 3.77e-12 60.8
Msa0550120 MtrunA17_Chr7g0267571 36.667 90 57 0 2 91 208 297 6.96e-12 62.0
Msa0550120 MtrunA17_Chr4g0050441 38.462 78 48 0 6 83 16 93 1.26e-11 61.2
Msa0550120 MtrunA17_Chr5g0429941 38.554 83 50 1 1 83 12 93 1.27e-11 60.8
Msa0550120 MtrunA17_Chr8g0360551 37.209 86 48 3 7 90 74 155 5.39e-11 59.7
Msa0550120 MtrunA17_Chr5g0419351 34.343 99 65 0 8 106 12 110 8.50e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550120 AT4G39260.3 74.725 91 23 0 1 91 1 91 3.02e-48 151
Msa0550120 AT2G21660.2 82.716 81 14 0 3 83 5 85 3.97e-46 148
Msa0550120 AT2G21660.1 82.716 81 14 0 3 83 5 85 6.64e-46 148
Msa0550120 AT4G39260.2 72.289 83 23 0 1 83 1 83 5.99e-43 139
Msa0550120 AT4G39260.1 72.289 83 23 0 1 83 1 83 2.19e-42 139
Msa0550120 AT4G39260.4 72.059 68 19 0 1 68 1 68 3.08e-34 116
Msa0550120 AT3G26420.1 35.321 218 80 4 3 159 4 221 8.15e-29 106
Msa0550120 AT5G61030.2 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0550120 AT5G61030.1 53.165 79 37 0 7 85 41 119 4.19e-26 101
Msa0550120 AT3G23830.2 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0550120 AT3G23830.1 53.191 94 44 0 7 100 36 129 5.37e-26 96.7
Msa0550120 AT5G50250.1 51.807 83 40 0 3 85 204 286 6.30e-25 97.4
Msa0550120 AT5G50250.1 40.000 90 54 0 5 94 112 201 1.01e-14 70.1
Msa0550120 AT4G13850.4 53.086 81 38 0 7 87 36 116 1.14e-24 92.8
Msa0550120 AT4G24770.1 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0550120 AT4G24770.1 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0550120 AT4G24770.2 50.000 82 41 0 6 87 244 325 1.31e-24 97.4
Msa0550120 AT4G24770.2 42.222 90 52 0 5 94 149 238 1.21e-15 73.2
Msa0550120 AT4G13850.1 52.525 99 47 0 7 105 36 134 3.75e-23 89.7
Msa0550120 AT5G04280.1 47.312 93 46 1 7 96 8 100 4.68e-23 93.2
Msa0550120 AT2G21690.1 49.438 89 40 1 2 85 3 91 9.31e-22 85.1
Msa0550120 AT2G37220.1 45.679 81 44 0 7 87 205 285 3.90e-21 87.4
Msa0550120 AT1G74230.1 57.647 85 35 1 7 91 35 118 4.56e-21 87.4
Msa0550120 AT3G53460.1 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0550120 AT3G53460.3 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0550120 AT3G53460.2 45.783 83 45 0 7 89 258 340 4.82e-21 88.2
Msa0550120 AT3G53460.4 45.783 83 45 0 7 89 279 361 7.04e-21 87.8
Msa0550120 AT5G06210.1 45.556 90 49 0 4 93 32 121 1.07e-20 83.2
Msa0550120 AT1G60650.2 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0550120 AT1G60650.1 41.121 107 62 1 4 109 10 116 1.12e-19 83.6
Msa0550120 AT4G13850.2 46.552 116 49 1 7 122 36 138 3.21e-19 79.7
Msa0550120 AT4G13850.3 44.628 121 45 1 7 127 36 134 2.15e-18 77.0
Msa0550120 AT5G47320.1 46.753 77 41 0 7 83 32 108 2.56e-17 75.9
Msa0550120 AT1G18630.1 52.083 96 46 0 7 102 37 132 2.77e-17 74.7
Msa0550120 AT1G22330.1 43.137 102 52 3 7 103 18 118 4.79e-17 76.6
Msa0550120 AT2G46780.1 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0550120 AT2G46780.2 46.053 76 40 1 7 82 23 97 7.97e-17 76.3
Msa0550120 AT4G13860.1 44.595 74 41 0 7 80 4 77 2.19e-16 70.5
Msa0550120 AT4G14300.1 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0550120 AT4G14300.1 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0550120 AT4G14300.2 37.975 79 47 1 7 85 7 83 3.55e-16 75.1
Msa0550120 AT4G14300.2 34.177 79 51 1 7 85 111 188 7.97e-11 59.7
Msa0550120 AT3G13224.3 38.710 93 51 2 1 89 1 91 4.35e-16 74.3
Msa0550120 AT3G13224.3 32.323 99 63 1 7 105 101 195 1.87e-14 70.1
Msa0550120 AT3G07810.2 33.028 109 71 1 7 115 7 113 8.47e-16 73.9
Msa0550120 AT3G07810.1 33.028 109 71 1 7 115 7 113 9.69e-16 73.9
Msa0550120 AT2G37510.1 45.946 74 40 0 7 80 35 108 1.04e-15 70.1
Msa0550120 AT5G59865.1 44.304 79 44 0 9 87 29 107 1.33e-15 68.9
Msa0550120 AT3G13224.2 39.759 83 48 1 7 89 20 100 1.44e-15 73.2
Msa0550120 AT3G13224.2 34.568 81 53 0 7 87 110 190 1.12e-14 70.5
Msa0550120 AT1G78260.1 44.444 81 43 2 3 82 13 92 1.65e-15 72.4
Msa0550120 AT1G76460.1 44.737 76 41 1 7 82 25 99 2.12e-15 72.0
Msa0550120 AT3G08000.1 44.048 84 47 0 7 90 42 125 2.64e-15 69.3
Msa0550120 AT4G26650.1 39.241 79 46 1 7 85 16 92 2.78e-15 72.4
Msa0550120 AT4G26650.2 39.241 79 46 1 7 85 13 89 2.97e-15 72.4
Msa0550120 AT1G78260.2 44.444 81 43 2 3 82 13 92 3.03e-15 71.2
Msa0550120 AT1G22910.1 36.364 99 56 3 3 94 9 107 4.12e-15 70.9
Msa0550120 AT1G20880.2 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0550120 AT1G20880.3 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0550120 AT1G20880.1 42.105 76 43 1 7 82 25 99 4.99e-15 70.9
Msa0550120 AT2G33410.1 32.558 86 56 1 7 92 7 90 5.83e-15 71.6
Msa0550120 AT2G33410.1 37.975 79 48 1 7 85 111 188 3.68e-12 63.5
Msa0550120 AT1G22910.2 36.364 99 56 3 3 94 9 107 6.11e-15 70.1
Msa0550120 AT5G55550.5 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0550120 AT5G55550.4 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0550120 AT5G55550.9 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0550120 AT5G55550.7 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0550120 AT5G55550.1 40.506 79 45 1 7 85 7 83 6.22e-15 71.6
Msa0550120 AT1G60000.1 40.244 82 49 0 2 83 173 254 7.05e-15 70.1
Msa0550120 AT5G55550.2 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0550120 AT5G55550.3 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0550120 AT5G55550.6 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0550120 AT5G55550.11 40.506 79 45 1 7 85 7 83 9.18e-15 71.2
Msa0550120 AT1G33470.3 37.500 104 58 3 1 98 1 103 1.53e-14 68.9
Msa0550120 AT1G33470.1 37.500 104 58 3 1 98 1 103 1.79e-14 68.9
Msa0550120 AT1G33470.2 37.500 104 58 3 1 98 1 103 1.80e-14 68.9
Msa0550120 AT5G19960.1 43.038 79 44 1 8 86 9 86 2.42e-14 69.7
Msa0550120 AT1G22910.3 36.364 99 56 3 3 94 9 107 4.40e-14 68.9
Msa0550120 AT3G13224.1 39.286 84 49 1 7 90 20 101 1.09e-13 66.6
Msa0550120 AT5G55550.8 42.254 71 39 1 7 77 7 75 1.88e-13 67.4
Msa0550120 AT5G51300.1 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0550120 AT5G51300.2 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0550120 AT5G51300.3 42.667 75 43 0 5 79 479 553 1.93e-13 67.4
Msa0550120 AT5G55550.10 42.254 71 39 1 7 77 7 75 2.38e-13 67.0
Msa0550120 AT3G54770.3 42.105 76 43 1 7 82 18 92 2.76e-13 65.9
Msa0550120 AT3G46020.1 37.662 77 48 0 7 83 8 84 2.99e-13 62.8
Msa0550120 AT3G54770.1 42.105 76 43 1 7 82 18 92 2.99e-13 65.9
Msa0550120 AT5G47620.1 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0550120 AT5G47620.2 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0550120 AT5G47620.5 35.443 79 49 1 7 85 7 83 3.65e-13 66.6
Msa0550120 AT5G40490.1 36.458 96 59 1 7 102 43 136 3.80e-13 66.2
Msa0550120 AT5G40490.1 32.292 96 63 1 7 100 131 226 1.14e-11 62.0
Msa0550120 AT1G17640.2 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0550120 AT1G17640.2 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0550120 AT1G17640.1 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0550120 AT1G17640.1 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0550120 AT1G17640.3 34.783 92 60 0 7 98 158 249 4.07e-13 66.2
Msa0550120 AT1G17640.3 38.750 80 47 1 7 86 67 144 1.17e-12 64.7
Msa0550120 AT3G52150.2 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0550120 AT3G52150.1 40.741 81 48 0 2 82 173 253 6.28e-13 64.7
Msa0550120 AT2G27330.1 31.579 76 52 0 9 84 24 99 6.86e-13 62.0
Msa0550120 AT1G73530.1 31.818 88 60 0 7 94 78 165 9.92e-13 63.2
Msa0550120 AT1G01080.1 37.349 83 47 2 1 81 207 286 1.61e-12 63.9
Msa0550120 AT5G53720.1 41.250 80 46 1 3 82 6 84 1.63e-12 60.8
Msa0550120 AT1G01080.2 37.349 83 47 2 1 81 208 287 1.74e-12 63.9
Msa0550120 AT5G64200.2 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0550120 AT5G64200.1 39.744 78 47 0 6 83 16 93 3.81e-12 63.2
Msa0550120 AT3G14100.1 39.744 78 47 0 5 82 143 220 4.53e-12 63.2
Msa0550120 AT5G47620.4 36.709 79 44 2 7 81 7 83 7.13e-12 62.8
Msa0550120 AT4G13860.2 45.763 59 32 0 22 80 2 60 1.12e-11 57.8
Msa0550120 AT1G54080.1 39.744 78 47 0 5 82 147 224 2.05e-11 61.2
Msa0550120 AT5G11412.1 42.169 83 44 3 2 82 47 127 2.56e-11 59.3
Msa0550120 AT4G36960.1 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0550120 AT4G36960.2 39.759 83 46 2 4 85 1 80 3.52e-11 60.8
Msa0550120 AT5G04280.3 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0550120 AT5G04280.4 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0550120 AT5G04280.5 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0550120 AT5G04280.2 46.774 62 30 1 38 96 1 62 3.58e-11 60.1
Msa0550120 AT5G40490.2 35.052 97 59 3 7 100 114 209 4.46e-11 60.5
Msa0550120 AT2G18510.1 30.081 123 85 1 8 129 114 236 5.95e-11 60.1
Msa0550120 AT5G54580.1 41.026 78 46 0 8 85 58 135 6.31e-11 57.8
Msa0550120 AT1G01080.3 40.000 55 33 0 1 55 207 261 9.93e-11 58.9

Find 91 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGTGGTGGTGGTGGATA+TGG 0.236629 4_1:+45532374 Msa0550120:CDS
AGGAGGTGATGGTGGTTATT+AGG 0.260943 4_1:+45532547 Msa0550120:CDS
GGTGGAAGCGGTGGTGGATA+TGG 0.268521 4_1:+45532416 Msa0550120:CDS
GGTGGAGGAGGTGGTGGATA+TGG 0.274998 4_1:+45532437 Msa0550120:CDS
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4_1:+45532203 Msa0550120:CDS
ATCATTAACGATCGTGAAAC+TGG 0.286078 4_1:+45532185 Msa0550120:CDS
GGCCGTGGTGGCGGTGGATA+TGG 0.292757 4_1:+45532353 Msa0550120:CDS
CACTTAAAACACAGATAATA+TGG 0.313021 4_1:-45532746 None:intergenic
AGCAATGAACGGTCAGGATT+TGG 0.313573 4_1:+45532271 Msa0550120:CDS
TGCTTCAATGGCATCGTTCA+TGG 0.319166 4_1:-45532252 None:intergenic
GGAGGCGGTGGTGGAGGATA+TGG 0.330951 4_1:+45532503 Msa0550120:CDS
GGAGGAGGTGGTGGATATGG+TGG 0.343759 4_1:+45532440 Msa0550120:CDS
GGTGGATATGGAGGTGGTGG+TGG 0.355416 4_1:+45532365 Msa0550120:CDS
GGTGGATATGGTGGAGGAGG+TGG 0.362609 4_1:+45532428 Msa0550120:CDS
ATGATTGTTATTCTAGATGT+TGG 0.374897 4_1:+45532589 Msa0550120:three_prime_UTR
GGTGGAGAGCGTCGTGGATA+TGG 0.378333 4_1:+45532395 Msa0550120:CDS
CTGACCGTTCATTGCTTCAA+TGG 0.378604 4_1:-45532264 None:intergenic
GGCGGAGGAGGCCGTGGTGG+CGG 0.382681 4_1:+45532344 Msa0550120:CDS
GGATATGGTGGAGGAGGTGG+TGG 0.390425 4_1:+45532431 Msa0550120:CDS
GGCGGTGGTGGAGGATATGG+TGG 0.396144 4_1:+45532506 Msa0550120:CDS
TACAGCAGAGGAGGCGGTGG+TGG 0.397231 4_1:+45532494 Msa0550120:CDS
GGATATGGAGGTGGTGGTGG+TGG 0.398226 4_1:+45532368 Msa0550120:CDS
CGCTACCACGGCTCTGAGCT+TGG 0.401331 4_1:-45532316 None:intergenic
TTAGGGTTTGGGAGAGAGAG+AGG 0.402475 4_1:-45531696 None:intergenic
TGATTGTTATTCTAGATGTT+GGG 0.408580 4_1:+45532590 Msa0550120:three_prime_UTR
GGCGGTGGATATGGAGGTGG+TGG 0.414719 4_1:+45532362 Msa0550120:CDS
GGTGGTGACCGCCGTGGTGG+AGG 0.417792 4_1:+45532464 Msa0550120:CDS
GGTGGTGGTGGTGGATATGG+TGG 0.419545 4_1:+45532377 Msa0550120:CDS
GGGAGAGAGAGAGGTTATAG+AGG 0.421172 4_1:-45531687 None:intergenic
GGAGGTGGTGGATATGGTGG+TGG 0.423953 4_1:+45532443 Msa0550120:CDS
GGTAGCGGTGGTGGCGGAGG+AGG 0.428094 4_1:+45532332 Msa0550120:CDS
GACCGCCGTGGTGGAGGTGG+TGG 0.428431 4_1:+45532470 Msa0550120:CDS
CGTGGTAGCGGTGGTGGCGG+AGG 0.433194 4_1:+45532329 Msa0550120:CDS
CAGAGCCGTGGTAGCGGTGG+TGG 0.439087 4_1:+45532323 Msa0550120:CDS
GGTGGTGGCGGAGGAGGCCG+TGG 0.439963 4_1:+45532338 Msa0550120:CDS
GGTGACCGCCGTGGTGGAGG+TGG 0.440014 4_1:+45532467 Msa0550120:CDS
AGCAGAGGAGGCGGTGGTGG+AGG 0.451847 4_1:+45532497 Msa0550120:CDS
GGATATGGTGGAAGCGGTGG+TGG 0.454383 4_1:+45532410 Msa0550120:CDS
GGTGGTGGATACAGCAGGGG+AGG 0.458420 4_1:+45532527 Msa0550120:CDS
GGAGGAGGCCGTGGTGGCGG+TGG 0.459076 4_1:+45532347 Msa0550120:CDS
AGCCGTGGTAGCGGTGGTGG+CGG 0.473368 4_1:+45532326 Msa0550120:CDS
GGAAGCGGTGGTGGATATGG+TGG 0.474255 4_1:+45532419 Msa0550120:CDS
GCTTCGTCGGAGGTCTTGCA+TGG 0.475042 4_1:+45531767 Msa0550120:CDS
GGTGGTGGAGGATATGGTGG+TGG 0.479646 4_1:+45532509 Msa0550120:CDS
GGTGGCGGAGGAGGCCGTGG+TGG 0.482575 4_1:+45532341 Msa0550120:CDS
GGTGGTGGATATGGTGGTGG+TGG 0.485578 4_1:+45532446 Msa0550120:CDS
GGTGGATACAGCAGGGGAGG+AGG 0.492965 4_1:+45532530 Msa0550120:CDS
ACAAAACACAGGTAATACTT+TGG 0.498137 4_1:-45532819 None:intergenic
ATGGTGGTGGTGGATACAGC+AGG 0.498899 4_1:+45532522 Msa0550120:CDS
GGTGGAGGATATGGTGGTGG+TGG 0.501369 4_1:+45532512 Msa0550120:CDS
GGATACAGCAGAGGAGGCGG+TGG 0.503411 4_1:+45532491 Msa0550120:CDS
CAGCCATTGAAAAGAGCAAA+GGG 0.505087 4_1:-45531729 None:intergenic
TACAGCAGGGGAGGAGGTGA+TGG 0.509384 4_1:+45532536 Msa0550120:CDS
GGTGGATACAGCAGAGGAGG+CGG 0.513743 4_1:+45532488 Msa0550120:CDS
CATTGAAGCAATGAACGGTC+AGG 0.516837 4_1:+45532265 Msa0550120:CDS
CAAGACCTCCGACGAAGCAT+CGG 0.524569 4_1:-45531762 None:intergenic
GTTGAATACCGATGCTTCGT+CGG 0.525860 4_1:+45531754 Msa0550120:CDS
CAAGCTCAGAGCCGTGGTAG+CGG 0.533686 4_1:+45532317 Msa0550120:CDS
CGGCGAGATCGTTGATTCCA+AGG 0.539852 4_1:+45531831 Msa0550120:CDS
AGCGGTGGTGGATATGGTGG+AGG 0.541831 4_1:+45532422 Msa0550120:CDS
GAGAAAGCCTTCTCTCAATA+CGG 0.543855 4_1:+45531811 Msa0550120:CDS
TTTCTCGAGAGCATCGTTGT+CGG 0.548634 4_1:-45531794 None:intergenic
CGTGAAACTGGACGATCAAG+AGG 0.551662 4_1:+45532197 Msa0550120:CDS
CGTGGTGGCGGTGGATATGG+AGG 0.555369 4_1:+45532356 Msa0550120:CDS
GGTGGTGGATATGGTGGAGG+AGG 0.560670 4_1:+45532425 Msa0550120:CDS
CGTCGTGGATATGGTGGAAG+CGG 0.562578 4_1:+45532404 Msa0550120:CDS
GGTGGTGGATACAGCAGAGG+AGG 0.562608 4_1:+45532485 Msa0550120:CDS
CGTGGATATGGTGGAAGCGG+TGG 0.562637 4_1:+45532407 Msa0550120:CDS
GGTGGTGGTGACCGCCGTGG+TGG 0.562802 4_1:+45532461 Msa0550120:CDS
CTCCATATCCACCGCCACCA+CGG 0.564840 4_1:-45532355 None:intergenic
GCTCAGAGCCGTGGTAGCGG+TGG 0.565666 4_1:+45532320 Msa0550120:CDS
AGCAGGGGAGGAGGTGATGG+TGG 0.566269 4_1:+45532539 Msa0550120:CDS
GGTGGCGGTGGATATGGAGG+TGG 0.573835 4_1:+45532359 Msa0550120:CDS
CTCGAGAGCATCGTTGTCGG+TGG 0.585909 4_1:-45531791 None:intergenic
GAATACCGATGCTTCGTCGG+AGG 0.590495 4_1:+45531757 Msa0550120:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.591814 4_1:+45531768 Msa0550120:CDS
CTCCGCCACCACCGCTACCA+CGG 0.595147 4_1:-45532328 None:intergenic
TCAGCCATTGAAAAGAGCAA+AGG 0.600296 4_1:-45531730 None:intergenic
TGGTGGTGGTGGATACAGCA+GGG 0.618235 4_1:+45532523 Msa0550120:CDS
ATGAACGGTCAGGATTTGGA+TGG 0.621464 4_1:+45532275 Msa0550120:CDS
GATCGTTATACACAAAACAC+AGG 0.633135 4_1:-45532830 None:intergenic
GGAGAGCGTCGTGGATATGG+TGG 0.640307 4_1:+45532398 Msa0550120:CDS
GTGAACCAAGCTCAGAGCCG+TGG 0.643330 4_1:+45532311 Msa0550120:CDS
GATCTCGCCGTATTGAGAGA+AGG 0.650761 4_1:-45531818 None:intergenic
GGTGGTGGTGGTGACCGCCG+TGG 0.665791 4_1:+45532458 Msa0550120:CDS
GGAGGTGGTGGATACAGCAG+AGG 0.669900 4_1:+45532482 Msa0550120:CDS
GATGCCATTGAAGCAATGAA+CGG 0.670960 4_1:+45532260 Msa0550120:CDS
GGTGGTGGTGGATACAGCAG+GGG 0.693203 4_1:+45532524 Msa0550120:CDS
TGTATCCACCACCTCCACCA+CGG 0.715403 4_1:-45532475 None:intergenic
GGATATGGTGGAGAGCGTCG+TGG 0.717480 4_1:+45532389 Msa0550120:CDS
ATCCACCACCTCCACCACGG+CGG 0.754503 4_1:-45532472 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_1:45532799-45532818 None:intergenic 10.0%
!!! TTAAGTTTATTTTAACTAAT+TGG - chr4_1:45532799-45532818 None:intergenic 10.0%
!!! TTTTTTTTCTTTTGATGTTT+TGG + chr4_1:45532125-45532144 Msa0550120:intron 15.0%
!!! TTTTTTTCTTTTGATGTTTT+GGG + chr4_1:45532126-45532145 Msa0550120:intron 15.0%
!!! TTTTTTTTCTTTTGATGTTT+TGG + chr4_1:45532125-45532144 Msa0550120:intron 15.0%
!!! TTTTTTTCTTTTGATGTTTT+GGG + chr4_1:45532126-45532145 Msa0550120:intron 15.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_1:45532589-45532608 Msa0550120:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_1:45532590-45532609 Msa0550120:three_prime_UTR 25.0%
! CACTTAAAACACAGATAATA+TGG - chr4_1:45532749-45532768 None:intergenic 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_1:45532795-45532814 None:intergenic 25.0%
!! ATGATTGTTATTCTAGATGT+TGG + chr4_1:45532589-45532608 Msa0550120:three_prime_UTR 25.0%
!! TGATTGTTATTCTAGATGTT+GGG + chr4_1:45532590-45532609 Msa0550120:three_prime_UTR 25.0%
! CACTTAAAACACAGATAATA+TGG - chr4_1:45532749-45532768 None:intergenic 25.0%
!!! GTTTATTTTAACTAATTGGC+AGG - chr4_1:45532795-45532814 None:intergenic 25.0%
GAAAAACTGAATTAGGGTTT+GGG - chr4_1:45531710-45531729 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+GGG - chr4_1:45531716-45531735 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_1:45531893-45531912 Msa0550120:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_1:45532067-45532086 Msa0550120:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_1:45532142-45532161 Msa0550120:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_1:45532632-45532651 Msa0550120:three_prime_UTR 30.0%
ACAAAACACAGGTAATACTT+TGG - chr4_1:45532822-45532841 None:intergenic 30.0%
GAAAAACTGAATTAGGGTTT+GGG - chr4_1:45531710-45531729 None:intergenic 30.0%
CAAAGGGAAAAACTGAATTA+GGG - chr4_1:45531716-45531735 None:intergenic 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG + chr4_1:45531893-45531912 Msa0550120:intron 30.0%
AACAAAAGTTGAAGATCGAT+CGG + chr4_1:45532067-45532086 Msa0550120:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG + chr4_1:45532142-45532161 Msa0550120:intron 30.0%
! TTTTTGTCACTGTTCTGTTA+TGG + chr4_1:45532632-45532651 Msa0550120:three_prime_UTR 30.0%
ACAAAACACAGGTAATACTT+TGG - chr4_1:45532822-45532841 None:intergenic 30.0%
GGAAAAACTGAATTAGGGTT+TGG - chr4_1:45531711-45531730 None:intergenic 35.0%
GCAAAGGGAAAAACTGAATT+AGG - chr4_1:45531717-45531736 None:intergenic 35.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_1:45531726-45531745 Msa0550120:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_1:45531883-45531902 Msa0550120:intron 35.0%
AACAGTAACACAGAACAGAT+CGG - chr4_1:45531941-45531960 None:intergenic 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_1:45532023-45532042 Msa0550120:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_1:45532041-45532060 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_1:45532078-45532097 Msa0550120:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_1:45532092-45532111 Msa0550120:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_1:45532185-45532204 Msa0550120:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_1:45532658-45532677 Msa0550120:three_prime_UTR 35.0%
GATCGTTATACACAAAACAC+AGG - chr4_1:45532833-45532852 None:intergenic 35.0%
GGAAAAACTGAATTAGGGTT+TGG - chr4_1:45531711-45531730 None:intergenic 35.0%
GCAAAGGGAAAAACTGAATT+AGG - chr4_1:45531717-45531736 None:intergenic 35.0%
! TTTCCCTTTGCTCTTTTCAA+TGG + chr4_1:45531726-45531745 Msa0550120:exon 35.0%
!!! GATCTGACACGATTTTGATT+CGG + chr4_1:45531883-45531902 Msa0550120:intron 35.0%
AACAGTAACACAGAACAGAT+CGG - chr4_1:45531941-45531960 None:intergenic 35.0%
TATTACTCACTCACACCAAT+AGG + chr4_1:45532023-45532042 Msa0550120:intron 35.0%
AAGAAGATGAACGTACCTAT+TGG - chr4_1:45532041-45532060 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG + chr4_1:45532078-45532097 Msa0550120:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG + chr4_1:45532092-45532111 Msa0550120:intron 35.0%
ATCATTAACGATCGTGAAAC+TGG + chr4_1:45532185-45532204 Msa0550120:CDS 35.0%
!! CGTTTTGATGTGTCTCTTAT+TGG + chr4_1:45532658-45532677 Msa0550120:three_prime_UTR 35.0%
GATCGTTATACACAAAACAC+AGG - chr4_1:45532833-45532852 None:intergenic 35.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_1:45531732-45531751 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_1:45531733-45531752 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_1:45531811-45531830 Msa0550120:CDS 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_1:45532260-45532279 Msa0550120:CDS 40.0%
CAGCCATTGAAAAGAGCAAA+GGG - chr4_1:45531732-45531751 None:intergenic 40.0%
TCAGCCATTGAAAAGAGCAA+AGG - chr4_1:45531733-45531752 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG + chr4_1:45531811-45531830 Msa0550120:CDS 40.0%
GATGCCATTGAAGCAATGAA+CGG + chr4_1:45532260-45532279 Msa0550120:CDS 40.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_1:45531754-45531773 Msa0550120:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_1:45531797-45531816 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_1:45531922-45531941 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_1:45532203-45532222 Msa0550120:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_1:45532255-45532274 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_1:45532267-45532286 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_1:45532265-45532284 Msa0550120:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_1:45532271-45532290 Msa0550120:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_1:45532275-45532294 Msa0550120:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_1:45532547-45532566 Msa0550120:CDS 45.0%
GTTGAATACCGATGCTTCGT+CGG + chr4_1:45531754-45531773 Msa0550120:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG - chr4_1:45531797-45531816 None:intergenic 45.0%
TCGGAGATCACAGATCAGAA+AGG - chr4_1:45531922-45531941 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG + chr4_1:45532203-45532222 Msa0550120:CDS 45.0%
TGCTTCAATGGCATCGTTCA+TGG - chr4_1:45532255-45532274 None:intergenic 45.0%
CTGACCGTTCATTGCTTCAA+TGG - chr4_1:45532267-45532286 None:intergenic 45.0%
CATTGAAGCAATGAACGGTC+AGG + chr4_1:45532265-45532284 Msa0550120:CDS 45.0%
AGCAATGAACGGTCAGGATT+TGG + chr4_1:45532271-45532290 Msa0550120:CDS 45.0%
ATGAACGGTCAGGATTTGGA+TGG + chr4_1:45532275-45532294 Msa0550120:CDS 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG + chr4_1:45532547-45532566 Msa0550120:CDS 45.0%
GGGAGAGAGAGAGGTTATAG+AGG - chr4_1:45531690-45531709 None:intergenic 50.0%
TTAGGGTTTGGGAGAGAGAG+AGG - chr4_1:45531699-45531718 None:intergenic 50.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_1:45531821-45531840 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_1:45531851-45531870 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_1:45531850-45531869 Msa0550120:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_1:45531858-45531877 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_1:45531856-45531875 Msa0550120:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_1:45532197-45532216 Msa0550120:CDS 50.0%
GGGAGAGAGAGAGGTTATAG+AGG - chr4_1:45531690-45531709 None:intergenic 50.0%
TTAGGGTTTGGGAGAGAGAG+AGG - chr4_1:45531699-45531718 None:intergenic 50.0%
GATCTCGCCGTATTGAGAGA+AGG - chr4_1:45531821-45531840 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG - chr4_1:45531851-45531870 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG + chr4_1:45531850-45531869 Msa0550120:intron 50.0%
CGAAGCCTGCAAGCAACAAA+CGG - chr4_1:45531858-45531877 None:intergenic 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG + chr4_1:45531856-45531875 Msa0550120:intron 50.0%
CGTGAAACTGGACGATCAAG+AGG + chr4_1:45532197-45532216 Msa0550120:CDS 50.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_1:45531757-45531776 Msa0550120:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_1:45531765-45531784 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_1:45531768-45531787 Msa0550120:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_1:45531831-45531850 Msa0550120:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_1:45531861-45531880 Msa0550120:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_1:45532404-45532423 Msa0550120:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_1:45532478-45532497 None:intergenic 55.0%
!! ATGGTGGTGGTGGATACAGC+AGG + chr4_1:45532522-45532541 Msa0550120:CDS 55.0%
!! TGGTGGTGGTGGATACAGCA+GGG + chr4_1:45532523-45532542 Msa0550120:CDS 55.0%
GAATACCGATGCTTCGTCGG+AGG + chr4_1:45531757-45531776 Msa0550120:CDS 55.0%
CAAGACCTCCGACGAAGCAT+CGG - chr4_1:45531765-45531784 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG + chr4_1:45531768-45531787 Msa0550120:CDS 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG + chr4_1:45531831-45531850 Msa0550120:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG + chr4_1:45531861-45531880 Msa0550120:intron 55.0%
! CGTCGTGGATATGGTGGAAG+CGG + chr4_1:45532404-45532423 Msa0550120:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG - chr4_1:45532478-45532497 None:intergenic 55.0%
!! ATGGTGGTGGTGGATACAGC+AGG + chr4_1:45532522-45532541 Msa0550120:CDS 55.0%
!! TGGTGGTGGTGGATACAGCA+GGG + chr4_1:45532523-45532542 Msa0550120:CDS 55.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_1:45531767-45531786 Msa0550120:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_1:45531794-45531813 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_1:45532311-45532330 Msa0550120:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_1:45532317-45532336 Msa0550120:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_1:45532358-45532377 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_1:45532365-45532384 Msa0550120:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_1:45532368-45532387 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_1:45532374-45532393 Msa0550120:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_1:45532377-45532396 Msa0550120:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_1:45532389-45532408 Msa0550120:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_1:45532395-45532414 Msa0550120:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_1:45532398-45532417 Msa0550120:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_1:45532407-45532426 Msa0550120:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_1:45532410-45532429 Msa0550120:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_1:45532416-45532435 Msa0550120:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_1:45532419-45532438 Msa0550120:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_1:45532422-45532441 Msa0550120:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_1:45532425-45532444 Msa0550120:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_1:45532428-45532447 Msa0550120:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_1:45532431-45532450 Msa0550120:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_1:45532437-45532456 Msa0550120:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_1:45532440-45532459 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_1:45532443-45532462 Msa0550120:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_1:45532446-45532465 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_1:45532482-45532501 Msa0550120:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_1:45532485-45532504 Msa0550120:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_1:45532488-45532507 Msa0550120:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_1:45532509-45532528 Msa0550120:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_1:45532512-45532531 Msa0550120:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+GGG + chr4_1:45532524-45532543 Msa0550120:CDS 60.0%
TACAGCAGGGGAGGAGGTGA+TGG + chr4_1:45532536-45532555 Msa0550120:CDS 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG + chr4_1:45531767-45531786 Msa0550120:CDS 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG - chr4_1:45531794-45531813 None:intergenic 60.0%
GTGAACCAAGCTCAGAGCCG+TGG + chr4_1:45532311-45532330 Msa0550120:CDS 60.0%
CAAGCTCAGAGCCGTGGTAG+CGG + chr4_1:45532317-45532336 Msa0550120:CDS 60.0%
CTCCATATCCACCGCCACCA+CGG - chr4_1:45532358-45532377 None:intergenic 60.0%
! GGTGGATATGGAGGTGGTGG+TGG + chr4_1:45532365-45532384 Msa0550120:CDS 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG + chr4_1:45532368-45532387 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG + chr4_1:45532374-45532393 Msa0550120:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG + chr4_1:45532377-45532396 Msa0550120:CDS 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG + chr4_1:45532389-45532408 Msa0550120:CDS 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG + chr4_1:45532395-45532414 Msa0550120:CDS 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG + chr4_1:45532398-45532417 Msa0550120:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG + chr4_1:45532407-45532426 Msa0550120:CDS 60.0%
! GGATATGGTGGAAGCGGTGG+TGG + chr4_1:45532410-45532429 Msa0550120:CDS 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG + chr4_1:45532416-45532435 Msa0550120:CDS 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG + chr4_1:45532419-45532438 Msa0550120:CDS 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG + chr4_1:45532422-45532441 Msa0550120:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr4_1:45532425-45532444 Msa0550120:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+TGG + chr4_1:45532428-45532447 Msa0550120:CDS 60.0%
! GGATATGGTGGAGGAGGTGG+TGG + chr4_1:45532431-45532450 Msa0550120:CDS 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG + chr4_1:45532437-45532456 Msa0550120:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr4_1:45532440-45532459 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG + chr4_1:45532443-45532462 Msa0550120:CDS 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG + chr4_1:45532446-45532465 Msa0550120:CDS 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG + chr4_1:45532482-45532501 Msa0550120:CDS 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG + chr4_1:45532485-45532504 Msa0550120:CDS 60.0%
GGTGGATACAGCAGAGGAGG+CGG + chr4_1:45532488-45532507 Msa0550120:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG + chr4_1:45532509-45532528 Msa0550120:CDS 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG + chr4_1:45532512-45532531 Msa0550120:CDS 60.0%
!! GGTGGTGGTGGATACAGCAG+GGG + chr4_1:45532524-45532543 Msa0550120:CDS 60.0%
TACAGCAGGGGAGGAGGTGA+TGG + chr4_1:45532536-45532555 Msa0550120:CDS 60.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_1:45532319-45532338 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_1:45532356-45532375 Msa0550120:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_1:45532359-45532378 Msa0550120:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_1:45532362-45532381 Msa0550120:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_1:45532475-45532494 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_1:45532491-45532510 Msa0550120:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_1:45532494-45532513 Msa0550120:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_1:45532503-45532522 Msa0550120:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_1:45532506-45532525 Msa0550120:CDS 65.0%
!! GGTGGTGGATACAGCAGGGG+AGG + chr4_1:45532527-45532546 Msa0550120:CDS 65.0%
GGTGGATACAGCAGGGGAGG+AGG + chr4_1:45532530-45532549 Msa0550120:CDS 65.0%
! AGCAGGGGAGGAGGTGATGG+TGG + chr4_1:45532539-45532558 Msa0550120:CDS 65.0%
!! CGCTACCACGGCTCTGAGCT+TGG - chr4_1:45532319-45532338 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG + chr4_1:45532356-45532375 Msa0550120:CDS 65.0%
! GGTGGCGGTGGATATGGAGG+TGG + chr4_1:45532359-45532378 Msa0550120:CDS 65.0%
GGCGGTGGATATGGAGGTGG+TGG + chr4_1:45532362-45532381 Msa0550120:CDS 65.0%
ATCCACCACCTCCACCACGG+CGG - chr4_1:45532475-45532494 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG + chr4_1:45532491-45532510 Msa0550120:CDS 65.0%
TACAGCAGAGGAGGCGGTGG+TGG + chr4_1:45532494-45532513 Msa0550120:CDS 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG + chr4_1:45532503-45532522 Msa0550120:CDS 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG + chr4_1:45532506-45532525 Msa0550120:CDS 65.0%
!! GGTGGTGGATACAGCAGGGG+AGG + chr4_1:45532527-45532546 Msa0550120:CDS 65.0%
GGTGGATACAGCAGGGGAGG+AGG + chr4_1:45532530-45532549 Msa0550120:CDS 65.0%
! AGCAGGGGAGGAGGTGATGG+TGG + chr4_1:45532539-45532558 Msa0550120:CDS 65.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_1:45532320-45532339 Msa0550120:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_1:45532323-45532342 Msa0550120:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_1:45532326-45532345 Msa0550120:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_1:45532331-45532350 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_1:45532353-45532372 Msa0550120:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_1:45532497-45532516 Msa0550120:CDS 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG + chr4_1:45532320-45532339 Msa0550120:CDS 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG + chr4_1:45532323-45532342 Msa0550120:CDS 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG + chr4_1:45532326-45532345 Msa0550120:CDS 70.0%
CTCCGCCACCACCGCTACCA+CGG - chr4_1:45532331-45532350 None:intergenic 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG + chr4_1:45532353-45532372 Msa0550120:CDS 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG + chr4_1:45532497-45532516 Msa0550120:CDS 70.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_1:45532329-45532348 Msa0550120:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_1:45532332-45532351 Msa0550120:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_1:45532458-45532477 Msa0550120:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_1:45532461-45532480 Msa0550120:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_1:45532464-45532483 Msa0550120:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_1:45532467-45532486 Msa0550120:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_1:45532470-45532489 Msa0550120:CDS 75.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG + chr4_1:45532329-45532348 Msa0550120:CDS 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG + chr4_1:45532332-45532351 Msa0550120:CDS 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG + chr4_1:45532458-45532477 Msa0550120:CDS 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG + chr4_1:45532461-45532480 Msa0550120:CDS 75.0%
GGTGGTGACCGCCGTGGTGG+AGG + chr4_1:45532464-45532483 Msa0550120:CDS 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG + chr4_1:45532467-45532486 Msa0550120:CDS 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG + chr4_1:45532470-45532489 Msa0550120:CDS 75.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_1:45532338-45532357 Msa0550120:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_1:45532341-45532360 Msa0550120:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_1:45532344-45532363 Msa0550120:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_1:45532347-45532366 Msa0550120:CDS 80.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG + chr4_1:45532338-45532357 Msa0550120:CDS 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG + chr4_1:45532341-45532360 Msa0550120:CDS 80.0%
! GGCGGAGGAGGCCGTGGTGG+CGG + chr4_1:45532344-45532363 Msa0550120:CDS 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG + chr4_1:45532347-45532366 Msa0550120:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45531666 45532872 45531666 ID=Msa0550120;Name=Msa0550120
chr4_1 mRNA 45531666 45532872 45531666 ID=Msa0550120-mRNA-1;Parent=Msa0550120;Name=Msa0550120-mRNA-1;_AED=0.09;_eAED=0.09;_QI=79|1|1|1|1|1|2|304|163
chr4_1 exon 45531666 45531852 45531666 ID=Msa0550120-mRNA-1:exon:11994;Parent=Msa0550120-mRNA-1
chr4_1 exon 45532185 45532872 45532185 ID=Msa0550120-mRNA-1:exon:11995;Parent=Msa0550120-mRNA-1
chr4_1 five_prime_UTR 45531666 45531744 45531666 ID=Msa0550120-mRNA-1:five_prime_utr;Parent=Msa0550120-mRNA-1
chr4_1 CDS 45531745 45531852 45531745 ID=Msa0550120-mRNA-1:cds;Parent=Msa0550120-mRNA-1
chr4_1 CDS 45532185 45532568 45532185 ID=Msa0550120-mRNA-1:cds;Parent=Msa0550120-mRNA-1
chr4_1 three_prime_UTR 45532569 45532872 45532569 ID=Msa0550120-mRNA-1:three_prime_utr;Parent=Msa0550120-mRNA-1
Gene Sequence

>Msa0550120

ATGGCTGATGTTGAATACCGATGCTTCGTCGGAGGTCTTGCATGGGCCACCGACAACGATGCTCTCGAGAAAGCCTTCTCTCAATACGGCGAGATCGTTGATTCCAAGATCATTAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTTGTGACATTCGCTAACGAGAAATCCATGAACGATGCCATTGAAGCAATGAACGGTCAGGATTTGGATGGACGTAACATCACTGTGAACCAAGCTCAGAGCCGTGGTAGCGGTGGTGGCGGAGGAGGCCGTGGTGGCGGTGGATATGGAGGTGGTGGTGGTGGATATGGTGGAGAGCGTCGTGGATATGGTGGAAGCGGTGGTGGATATGGTGGAGGAGGTGGTGGATATGGTGGTGGTGGTGACCGCCGTGGTGGAGGTGGTGGATACAGCAGAGGAGGCGGTGGTGGAGGATATGGTGGTGGTGGATACAGCAGGGGAGGAGGTGATGGTGGTTATTAG
Protein sequence

>Msa0550120

MADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDLDGRNITVNQAQSRGSGGGGGGRGGGGYGGGGGGYGGERRGYGGSGGGYGGGGGGYGGGGDRRGGGGGYSRGGGGGGYGGGGYSRGGGDGGY*