AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0745970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0745970 MtrunA17_Chr5g0441161 97.309 223 5 1 1 222 1 223 4.26e-155 429
Msa0745970 MtrunA17_Chr4g0008681 63.636 231 75 3 1 222 1 231 1.17e-75 228
Msa0745970 MtrunA17_Chr2g0305121 63.203 231 76 4 1 222 1 231 2.22e-73 223
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0745970 AT5G12110.1 70.175 228 62 3 1 222 1 228 8.50e-101 292
Msa0745970 AT5G19510.1 74.107 224 56 2 1 222 1 224 3.75e-90 265
Msa0745970 AT2G18110.1 57.642 229 85 4 5 222 4 231 2.14e-71 217
Msa0745970 AT1G30230.1 59.211 228 83 4 5 222 4 231 1.22e-68 210
Msa0745970 AT1G30230.2 59.211 228 83 4 5 222 4 231 2.58e-68 211

Find 98 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCACCATAACTGCTTTAT+TGG 0.144642 5_1:-67944428 None:intergenic
GAGAGTTGTGTGTATGATTT+AGG 0.162453 5_1:+67941226 Msa0745970:five_prime_UTR
TAGCAAGGGCTCATAATTTA+AGG 0.165402 5_1:-67944615 None:intergenic
AATCGTCATATTGGCGTTCT+TGG 0.231833 5_1:+67944556 Msa0745970:three_prime_UTR
CTCTGGACATACTCGTTAAT+TGG 0.274565 5_1:-67944027 None:intergenic
GTCGTTTCTTCTTTCATGTT+TGG 0.283824 5_1:+67944158 Msa0745970:three_prime_UTR
TCTAATCAGCCGCTTGAAAA+TGG 0.306365 5_1:-67944095 None:intergenic
CAAAGTGTATGGATATGTTT+TGG 0.310672 5_1:+67941815 Msa0745970:CDS
TTAGCAGGTGCTCCTGCAGC+TGG 0.312184 5_1:-67942084 None:intergenic
TTACTCTCTTTCTTCTTTGC+GGG 0.314961 5_1:-67943541 None:intergenic
CTGAGAAGGTAACAGCCATT+AGG 0.352405 5_1:-67941365 None:intergenic
AGAGAGATGGAAATATTTAA+TGG 0.353217 5_1:+67944223 Msa0745970:three_prime_UTR
TGATAAATCAAACGGGGATA+TGG 0.353507 5_1:-67944698 None:intergenic
GTTGGAGTTCATGGTAATAA+TGG 0.353706 5_1:+67944490 Msa0745970:three_prime_UTR
CTGAGGCACAATCGTCATAT+TGG 0.364569 5_1:+67944547 Msa0745970:three_prime_UTR
TTGTGTATCGATTCAATAAA+CGG 0.373231 5_1:-67944342 None:intergenic
TGAGAAGGTAACAGCCATTA+GGG 0.375051 5_1:-67941364 None:intergenic
GATGATGATCTTGACCTCTT+TGG 0.382817 5_1:+67943463 Msa0745970:CDS
AGAGTTGTGTGTATGATTTA+GGG 0.383270 5_1:+67941227 Msa0745970:five_prime_UTR
ACATGCTCCCCAGGTTAAAC+CGG 0.384492 5_1:-67943781 None:intergenic
TCCCTCAATGATTTCCTCTC+TGG 0.387083 5_1:+67941414 Msa0745970:CDS
TTGAAATTCCCGGTTTAACC+TGG 0.398982 5_1:+67943772 Msa0745970:CDS
CTTACTCTCTTTCTTCTTTG+CGG 0.404225 5_1:-67943542 None:intergenic
AAACGACAGAGTGGAGGAGA+AGG 0.405632 5_1:-67944142 None:intergenic
AGCAAGGGCTCATAATTTAA+GGG 0.409284 5_1:-67944614 None:intergenic
GTTCGCAGCGTTGAAATTCC+CGG 0.412225 5_1:+67943762 Msa0745970:CDS
TGAAAGTTGAGAGTGAGTTT+TGG 0.416192 5_1:-67941289 None:intergenic
CGATTCATCGCAAACCCTAA+TGG 0.420045 5_1:+67941350 Msa0745970:exon
CCATTGTGTCACCAAATGAT+TGG 0.425796 5_1:+67944582 Msa0745970:three_prime_UTR
AAAATTTAAATAGAAGCATA+TGG 0.427426 5_1:-67944302 None:intergenic
CCATGCCAATAAAGCAGTTA+TGG 0.428995 5_1:+67944423 Msa0745970:three_prime_UTR
CAATATGACGATTGTGCCTC+AGG 0.437306 5_1:-67944546 None:intergenic
GGAAAACCATGCCAATCATT+TGG 0.439116 5_1:-67944593 None:intergenic
AAGGATGATATCAAAGTGTA+TGG 0.446962 5_1:+67941804 Msa0745970:CDS
TATACTGCTGGAATCATTCA+AGG 0.447457 5_1:+67944639 Msa0745970:three_prime_UTR
TAAGAAGGCAGCAGAGGAAA+GGG 0.453012 5_1:+67943504 Msa0745970:CDS
AGATACAAGATCGTCAATAA+TGG 0.472820 5_1:-67943968 None:intergenic
CAAACACCATGCACATTTGT+GGG 0.474792 5_1:+67944264 Msa0745970:three_prime_UTR
ATTGGCATGGCATATGCAAA+AGG 0.478361 5_1:-67944410 None:intergenic
TCAAACACCATGCACATTTG+TGG 0.479585 5_1:+67944263 Msa0745970:three_prime_UTR
CATTAGGGTTTGCGATGAAT+CGG 0.480889 5_1:-67941349 None:intergenic
AGTGGAAGTGCTCCAGCTGC+AGG 0.481359 5_1:+67942072 Msa0745970:CDS
TGCATTTGTTACAGCCAAGC+TGG 0.491896 5_1:+67943903 Msa0745970:intron
AAGCTGGTTGCTGTTGGATA+CGG 0.492710 5_1:+67943919 Msa0745970:CDS
TCCAGAGAGGAAATCATTGA+GGG 0.494384 5_1:-67941415 None:intergenic
AGCCCTTGCTAGTATACTGC+TGG 0.500730 5_1:+67944627 Msa0745970:three_prime_UTR
TGAGACAGACATGAAGAAGC+TGG 0.511507 5_1:+67943731 Msa0745970:CDS
TGAAACAGAGGAGGATAAGA+AGG 0.514826 5_1:+67943489 Msa0745970:CDS
ATAAGAAGGCAGCAGAGGAA+AGG 0.514986 5_1:+67943503 Msa0745970:CDS
TCATCATCATCTTCAGCAGC+AGG 0.517729 5_1:-67943448 None:intergenic
GCTGATTAGACTGTTATTTG+CGG 0.518900 5_1:+67944108 Msa0745970:three_prime_UTR
CCATAACTGCTTTATTGGCA+TGG 0.526932 5_1:-67944423 None:intergenic
ATGCGACAATGTCACAGCTC+TGG 0.527719 5_1:-67944044 None:intergenic
ATGTCTGTCTCATCATCCCA+TGG 0.532053 5_1:-67943720 None:intergenic
ATTGTATGGAGTGAGAGAGA+TGG 0.536485 5_1:+67944210 Msa0745970:three_prime_UTR
TTCTGCTTGATGTTAAGCCA+TGG 0.537433 5_1:+67943703 Msa0745970:CDS
TCTCTTCACATCTTGCTGCA+AGG 0.539260 5_1:+67941886 Msa0745970:CDS
TGAAATTCCCGGTTTAACCT+GGG 0.541002 5_1:+67943773 Msa0745970:CDS
TGGCTTAACATCAAGCAGAA+TGG 0.546092 5_1:-67943700 None:intergenic
AGACATGAAGAAGCTGGAAG+AGG 0.547223 5_1:+67943737 Msa0745970:CDS
TTCCAGAGAGGAAATCATTG+AGG 0.548339 5_1:-67941416 None:intergenic
TCATGTTTGGTGTGTTTCAG+TGG 0.549318 5_1:+67944171 Msa0745970:three_prime_UTR
GAGATACATACAGCTTTAGC+AGG 0.553332 5_1:-67942099 None:intergenic
CTGTGAGTCTTTCTTCAATG+AGG 0.560347 5_1:-67943999 None:intergenic
ATCGTACCAACCAGCAACAT+TGG 0.561284 5_1:-67941859 None:intergenic
AACGACAGAGTGGAGGAGAA+GGG 0.565824 5_1:-67944141 None:intergenic
CTCAGATCTTCACACAGAAG+AGG 0.570425 5_1:+67941383 Msa0745970:CDS
ACATTGGCAAAAGCATCACT+TGG 0.572710 5_1:-67941843 None:intergenic
GGAGGATAAGAAGGCAGCAG+AGG 0.581848 5_1:+67943498 Msa0745970:CDS
CCAATCATTTGGTGACACAA+TGG 0.582811 5_1:-67944582 None:intergenic
TCAGATCTTCACACAGAAGA+GGG 0.583876 5_1:+67941384 Msa0745970:CDS
GCTCAAGGTGTGAAATTCAG+TGG 0.585505 5_1:+67942054 Msa0745970:CDS
AGTTTCCCTGGAAAAGCTCA+AGG 0.588553 5_1:+67942039 Msa0745970:intron
ATTCCAGCAGTATACTAGCA+AGG 0.592946 5_1:-67944630 None:intergenic
TCTGCTTGATGTTAAGCCAT+GGG 0.593895 5_1:+67943704 Msa0745970:CDS
CGGGGATATGGTGCTATCCA+CGG 0.593943 5_1:-67944686 None:intergenic
TGTTTGGTGTGTTTCAGTGG+TGG 0.597058 5_1:+67944174 Msa0745970:three_prime_UTR
CATGCTCCCCAGGTTAAACC+GGG 0.597168 5_1:-67943780 None:intergenic
TAGGGTTTGCGATGAATCGG+TGG 0.599404 5_1:-67941346 None:intergenic
ACAGCCAAGCTGGTTGCTGT+TGG 0.600555 5_1:+67943913 Msa0745970:intron
TTCTGTGTGAAGATCTGAGA+AGG 0.604138 5_1:-67941379 None:intergenic
GAAATTCCCGGTTTAACCTG+GGG 0.605534 5_1:+67943774 Msa0745970:CDS
TTCCAGCAGTATACTAGCAA+GGG 0.614350 5_1:-67944629 None:intergenic
CCTCTGTTTCATCACCAAAG+AGG 0.617705 5_1:-67943477 None:intergenic
GAAGGCAGCAGAGGAAAGGG+AGG 0.617890 5_1:+67943507 Msa0745970:CDS
GGTTTGCGATGAATCGGTGG+CGG 0.621249 5_1:-67941343 None:intergenic
GACAGAGTGGAGGAGAAGGG+AGG 0.622905 5_1:-67944138 None:intergenic
GTATCCAACAGCAACCAGCT+TGG 0.624167 5_1:-67943917 None:intergenic
GTGTCACCAAATGATTGGCA+TGG 0.627955 5_1:+67944587 Msa0745970:three_prime_UTR
ATGGTAATAATGGTTACATG+TGG 0.633867 5_1:+67944500 Msa0745970:three_prime_UTR
ATTCAGGGATCAATTGACAA+AGG 0.634362 5_1:+67941785 Msa0745970:intron
CCTCTTTGGTGATGAAACAG+AGG 0.645851 5_1:+67943477 Msa0745970:CDS
TGCCAATAAAGCAGTTATGG+TGG 0.646400 5_1:+67944426 Msa0745970:three_prime_UTR
GTAAAGTTGCGATCATGCCG+TGG 0.658532 5_1:+67944669 Msa0745970:three_prime_UTR
AAGAAGAAACGACAGAGTGG+AGG 0.662799 5_1:-67944148 None:intergenic
TGAAAGAAGAAACGACAGAG+TGG 0.667900 5_1:-67944151 None:intergenic
CTTTGGTGATGAAACAGAGG+AGG 0.675786 5_1:+67943480 Msa0745970:CDS
GCTAATCCCACAAATGTGCA+TGG 0.688751 5_1:-67944270 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAGAAAAAAAAAATAATAT+TGG + chr5_1:67941204-67941223 Msa0745970:five_prime_UTR 10.0%
!! AATATAAAGATCAATAATTA+AGG - chr5_1:67941938-67941957 None:intergenic 10.0%
!! TTTATATTGAAATATGAATT+TGG + chr5_1:67941950-67941969 Msa0745970:intron 10.0%
!! AATTTCAATAATAAAATTGT+TGG - chr5_1:67942929-67942948 None:intergenic 10.0%
!!! TTCTATTTTATTGTTTTGAA+TGG + chr5_1:67941718-67941737 Msa0745970:intron 15.0%
!!! TCTATTTTATTGTTTTGAAT+GGG + chr5_1:67941719-67941738 Msa0745970:intron 15.0%
!!! AAAATTTAAGTTTGAGTTTT+TGG + chr5_1:67944072-67944091 Msa0745970:exon 15.0%
!!! AAAATTTAAATAGAAGCATA+TGG - chr5_1:67944305-67944324 None:intergenic 15.0%
!!! AAATATATTTTTTTCCAGAG+AGG - chr5_1:67941431-67941450 None:intergenic 20.0%
!! TCTGGAAAAAAATATATTTC+TGG + chr5_1:67941432-67941451 Msa0745970:CDS 20.0%
!! CTGGAAAAAAATATATTTCT+GGG + chr5_1:67941433-67941452 Msa0745970:CDS 20.0%
!! TAAAAAAAGTAGAAATTGAG+AGG - chr5_1:67941471-67941490 None:intergenic 20.0%
!!! TTTTTATTGTTTCTGAGATT+TGG + chr5_1:67941515-67941534 Msa0745970:intron 20.0%
!!! TTTTATTGTTTCTGAGATTT+GGG + chr5_1:67941516-67941535 Msa0745970:intron 20.0%
!! ATTCGAATCAATAAAATAGA+AGG - chr5_1:67941574-67941593 None:intergenic 20.0%
!!! TTTTTTACTGTTTTGAGATT+TGG + chr5_1:67941610-67941629 Msa0745970:intron 20.0%
!!! TTTTTACTGTTTTGAGATTT+GGG + chr5_1:67941611-67941630 Msa0745970:intron 20.0%
!!! CTATTTTATTGTTTTGAATG+GGG + chr5_1:67941720-67941739 Msa0745970:intron 20.0%
!! ACACGAAAAATTAAAATTGA+GGG - chr5_1:67942124-67942143 None:intergenic 20.0%
!! AACACGAAAAATTAAAATTG+AGG - chr5_1:67942125-67942144 None:intergenic 20.0%
!! GTTATTGTCAATTGTAAATA+TGG + chr5_1:67942599-67942618 Msa0745970:intron 20.0%
!!! GAGTCTTTGATTTTATTTAT+TGG + chr5_1:67942719-67942738 Msa0745970:intron 20.0%
!! AAAATTACAAAATGTCTTCT+AGG - chr5_1:67942764-67942783 None:intergenic 20.0%
!!! GAAATAAAAATAGAGTACTT+TGG - chr5_1:67942876-67942895 None:intergenic 20.0%
!! AATCATTGCATGTTTATTAT+AGG + chr5_1:67943049-67943068 Msa0745970:intron 20.0%
!! AAAAATTTATAACGACTAGA+AGG - chr5_1:67943572-67943591 None:intergenic 20.0%
!!! TCATTAAGTGATATTTTTAC+AGG + chr5_1:67943666-67943685 Msa0745970:intron 20.0%
!!! GGTTTTTCAAATTCTATGTT+GGG + chr5_1:67941537-67941556 Msa0745970:intron 25.0%
!! TTGATTCGAATTGAAAAGAT+TGG + chr5_1:67941583-67941602 Msa0745970:intron 25.0%
!!! TTTTTACTGTTTCTGAGATT+TGG + chr5_1:67941660-67941679 Msa0745970:intron 25.0%
!!! TTTTACTGTTTCTGAGATTT+GGG + chr5_1:67941661-67941680 Msa0745970:intron 25.0%
!!! TTTTTACTGTTTCTGAGATT+TGG + chr5_1:67941660-67941679 Msa0745970:intron 25.0%
!!! TTTTACTGTTTCTGAGATTT+GGG + chr5_1:67941661-67941680 Msa0745970:intron 25.0%
!! ATTTGGGTTATTGAAATTCA+GGG + chr5_1:67941770-67941789 Msa0745970:intron 25.0%
!!! CATTTTTCAGTAATATACTG+TGG + chr5_1:67942230-67942249 Msa0745970:intron 25.0%
!! CATCCAACATAATTTGAAAT+CGG + chr5_1:67942300-67942319 Msa0745970:intron 25.0%
!! CTTTCTGAAAATAAATGACT+AGG - chr5_1:67942434-67942453 None:intergenic 25.0%
! ATAAATAATCCACACGAAAA+AGG + chr5_1:67942805-67942824 Msa0745970:intron 25.0%
!!! TGGTTTTTTAGAGTAGTATT+AGG + chr5_1:67942975-67942994 Msa0745970:intron 25.0%
!!! GGTTTTTTAGAGTAGTATTA+GGG + chr5_1:67942976-67942995 Msa0745970:intron 25.0%
! CATGTTTATTATAGGCAATT+AGG + chr5_1:67943057-67943076 Msa0745970:intron 25.0%
!! TTTTGACATGACATACTATA+AGG + chr5_1:67943171-67943190 Msa0745970:intron 25.0%
! CTACTAACTAATTTGATCAT+TGG + chr5_1:67943405-67943424 Msa0745970:intron 25.0%
! ATCATTGGAATTTATTTGTC+AGG + chr5_1:67943420-67943439 Msa0745970:intron 25.0%
!!! TTAAGTGATATTTTTACAGG+TGG + chr5_1:67943669-67943688 Msa0745970:intron 25.0%
!!! GTTTTGTTACAAGAATTGTA+TGG + chr5_1:67944196-67944215 Msa0745970:three_prime_UTR 25.0%
! AGAGAGATGGAAATATTTAA+TGG + chr5_1:67944223-67944242 Msa0745970:three_prime_UTR 25.0%
!!! TTGTGTATCGATTCAATAAA+CGG - chr5_1:67944345-67944364 None:intergenic 25.0%
!! TGATTTTCATAAATATCCTG+AGG + chr5_1:67944530-67944549 Msa0745970:three_prime_UTR 25.0%
AGAGTTGTGTGTATGATTTA+GGG + chr5_1:67941227-67941246 Msa0745970:five_prime_UTR 30.0%
! TAGAAATTGAGAGGATTTTG+GGG - chr5_1:67941462-67941481 None:intergenic 30.0%
! GTAGAAATTGAGAGGATTTT+GGG - chr5_1:67941463-67941482 None:intergenic 30.0%
! AGTAGAAATTGAGAGGATTT+TGG - chr5_1:67941464-67941483 None:intergenic 30.0%
!! GGGTTTTTCAAATTCTATGT+TGG + chr5_1:67941536-67941555 Msa0745970:intron 30.0%
! GATTTGGGTTATTGAAATTC+AGG + chr5_1:67941769-67941788 Msa0745970:intron 30.0%
AAGGATGATATCAAAGTGTA+TGG + chr5_1:67941804-67941823 Msa0745970:CDS 30.0%
! CAAAGTGTATGGATATGTTT+TGG + chr5_1:67941815-67941834 Msa0745970:CDS 30.0%
!!! TTTTGTATGATTAGTTTCCC+TGG + chr5_1:67942027-67942046 Msa0745970:intron 30.0%
TGTATTTCTTAGAGTAACAC+TGG + chr5_1:67942204-67942223 Msa0745970:intron 30.0%
GTATTTCTTAGAGTAACACT+GGG + chr5_1:67942205-67942224 Msa0745970:intron 30.0%
!! ATGCCGATTTCAAATTATGT+TGG - chr5_1:67942306-67942325 None:intergenic 30.0%
GAAAAGCTTACAGGTTTAAA+GGG - chr5_1:67942376-67942395 None:intergenic 30.0%
TGAAAAGCTTACAGGTTTAA+AGG - chr5_1:67942377-67942396 None:intergenic 30.0%
ACTTAAGATGAAAAGCTTAC+AGG - chr5_1:67942385-67942404 None:intergenic 30.0%
!! TTGTTTTTCACTTTTGAGCA+TGG - chr5_1:67942648-67942667 None:intergenic 30.0%
AGAATACAAAACACTAGAGT+TGG - chr5_1:67942683-67942702 None:intergenic 30.0%
!!! AAGCATAGTTTTGAGAAAAG+TGG + chr5_1:67943623-67943642 Msa0745970:intron 30.0%
AGATACAAGATCGTCAATAA+TGG - chr5_1:67943971-67943990 None:intergenic 30.0%
GAACTCCAACAACAAAAAAT+CGG - chr5_1:67944480-67944499 None:intergenic 30.0%
!!! TTTTTTGTTGTTGGAGTTCA+TGG + chr5_1:67944481-67944500 Msa0745970:three_prime_UTR 30.0%
ATGGTAATAATGGTTACATG+TGG + chr5_1:67944500-67944519 Msa0745970:three_prime_UTR 30.0%
GAGAGTTGTGTGTATGATTT+AGG + chr5_1:67941226-67941245 Msa0745970:five_prime_UTR 35.0%
!!! GAGTTTTGGGCTTCAATAAT+TGG - chr5_1:67941278-67941297 None:intergenic 35.0%
! GAAAGTTGAGAGTGAGTTTT+GGG - chr5_1:67941291-67941310 None:intergenic 35.0%
! TGAAAGTTGAGAGTGAGTTT+TGG - chr5_1:67941292-67941311 None:intergenic 35.0%
!! GGGTTTTTCAAATTCTATGC+TGG + chr5_1:67941631-67941650 Msa0745970:intron 35.0%
ATTCAGGGATCAATTGACAA+AGG + chr5_1:67941785-67941804 Msa0745970:intron 35.0%
TTATGTCACGTAGAGTTGTA+TGG + chr5_1:67942168-67942187 Msa0745970:intron 35.0%
!! AAGTATTGTCCTTTTTCGTG+TGG - chr5_1:67942817-67942836 None:intergenic 35.0%
GCAATGATTAAAAACATGCG+TGG - chr5_1:67943038-67943057 None:intergenic 35.0%
GACATGACATACTATAAGGA+TGG + chr5_1:67943175-67943194 Msa0745970:intron 35.0%
ACTTGGTAAACATCTCTATC+AGG - chr5_1:67943228-67943247 None:intergenic 35.0%
TAGCTAACAACTATGTCACT+TGG - chr5_1:67943245-67943264 None:intergenic 35.0%
GACATAGTTGTTAGCTAAGT+TGG + chr5_1:67943248-67943267 Msa0745970:intron 35.0%
!! GCTAAGTTGGTAACTATGAA+AGG + chr5_1:67943261-67943280 Msa0745970:intron 35.0%
CCTAAACTTCACAACAACTT+GGG - chr5_1:67943347-67943366 None:intergenic 35.0%
ACCTAAACTTCACAACAACT+TGG - chr5_1:67943348-67943367 None:intergenic 35.0%
AGTTGTATTGAAGTCACTGA+AGG + chr5_1:67943380-67943399 Msa0745970:intron 35.0%
TTACTCTCTTTCTTCTTTGC+GGG - chr5_1:67943544-67943563 None:intergenic 35.0%
CTTACTCTCTTTCTTCTTTG+CGG - chr5_1:67943545-67943564 None:intergenic 35.0%
AGAAAAGTGGTCATTGAGTT+TGG + chr5_1:67943636-67943655 Msa0745970:intron 35.0%
CAATTCCTATGTGACTGATT+TGG + chr5_1:67943875-67943894 Msa0745970:intron 35.0%
!!! AGTTTTTGGCCATTTTCAAG+CGG + chr5_1:67944086-67944105 Msa0745970:three_prime_UTR 35.0%
! GCTGATTAGACTGTTATTTG+CGG + chr5_1:67944108-67944127 Msa0745970:three_prime_UTR 35.0%
GTCGTTTCTTCTTTCATGTT+TGG + chr5_1:67944158-67944177 Msa0745970:three_prime_UTR 35.0%
GTTGGAGTTCATGGTAATAA+TGG + chr5_1:67944490-67944509 Msa0745970:three_prime_UTR 35.0%
AGCAAGGGCTCATAATTTAA+GGG - chr5_1:67944617-67944636 None:intergenic 35.0%
TAGCAAGGGCTCATAATTTA+AGG - chr5_1:67944618-67944637 None:intergenic 35.0%
TATACTGCTGGAATCATTCA+AGG + chr5_1:67944639-67944658 Msa0745970:three_prime_UTR 35.0%
CATTAGGGTTTGCGATGAAT+CGG - chr5_1:67941352-67941371 None:intergenic 40.0%
TGAGAAGGTAACAGCCATTA+GGG - chr5_1:67941367-67941386 None:intergenic 40.0%
TTCTGTGTGAAGATCTGAGA+AGG - chr5_1:67941382-67941401 None:intergenic 40.0%
TCAGATCTTCACACAGAAGA+GGG + chr5_1:67941384-67941403 Msa0745970:CDS 40.0%
TCCAGAGAGGAAATCATTGA+GGG - chr5_1:67941418-67941437 None:intergenic 40.0%
TTCCAGAGAGGAAATCATTG+AGG - chr5_1:67941419-67941438 None:intergenic 40.0%
!! ACATTGGCAAAAGCATCACT+TGG - chr5_1:67941846-67941865 None:intergenic 40.0%
! GAGATACATACAGCTTTAGC+AGG - chr5_1:67942102-67942121 None:intergenic 40.0%
TTTCAGCCAAGATAGCAACA+TGG + chr5_1:67942951-67942970 Msa0745970:intron 40.0%
AACCATCCATGTTGCTATCT+TGG - chr5_1:67942960-67942979 None:intergenic 40.0%
TGCGTGGATCTCATTAAGAA+AGG - chr5_1:67943022-67943041 None:intergenic 40.0%
CTATCAGGTTCAACTAACAC+AGG - chr5_1:67943213-67943232 None:intergenic 40.0%
CCCAAGTTGTTGTGAAGTTT+AGG + chr5_1:67943344-67943363 Msa0745970:intron 40.0%
GATGATGATCTTGACCTCTT+TGG + chr5_1:67943463-67943482 Msa0745970:CDS 40.0%
TGAAACAGAGGAGGATAAGA+AGG + chr5_1:67943489-67943508 Msa0745970:CDS 40.0%
TGGCTTAACATCAAGCAGAA+TGG - chr5_1:67943703-67943722 None:intergenic 40.0%
TTCTGCTTGATGTTAAGCCA+TGG + chr5_1:67943703-67943722 Msa0745970:CDS 40.0%
TCTGCTTGATGTTAAGCCAT+GGG + chr5_1:67943704-67943723 Msa0745970:CDS 40.0%
TTGAAATTCCCGGTTTAACC+TGG + chr5_1:67943772-67943791 Msa0745970:CDS 40.0%
TGAAATTCCCGGTTTAACCT+GGG + chr5_1:67943773-67943792 Msa0745970:CDS 40.0%
! TTTTAACATACATGCTCCCC+AGG - chr5_1:67943793-67943812 None:intergenic 40.0%
ACAAGCCAAATCAGTCACAT+AGG - chr5_1:67943883-67943902 None:intergenic 40.0%
CTGTGAGTCTTTCTTCAATG+AGG - chr5_1:67944002-67944021 None:intergenic 40.0%
CTCTGGACATACTCGTTAAT+TGG - chr5_1:67944030-67944049 None:intergenic 40.0%
TCTAATCAGCCGCTTGAAAA+TGG - chr5_1:67944098-67944117 None:intergenic 40.0%
TGAAAGAAGAAACGACAGAG+TGG - chr5_1:67944154-67944173 None:intergenic 40.0%
!! TCATGTTTGGTGTGTTTCAG+TGG + chr5_1:67944171-67944190 Msa0745970:three_prime_UTR 40.0%
ATTGTATGGAGTGAGAGAGA+TGG + chr5_1:67944210-67944229 Msa0745970:three_prime_UTR 40.0%
TCAAACACCATGCACATTTG+TGG + chr5_1:67944263-67944282 Msa0745970:three_prime_UTR 40.0%
CAAACACCATGCACATTTGT+GGG + chr5_1:67944264-67944283 Msa0745970:three_prime_UTR 40.0%
ATTGGCATGGCATATGCAAA+AGG - chr5_1:67944413-67944432 None:intergenic 40.0%
! CCATAACTGCTTTATTGGCA+TGG - chr5_1:67944426-67944445 None:intergenic 40.0%
CCATGCCAATAAAGCAGTTA+TGG + chr5_1:67944423-67944442 Msa0745970:three_prime_UTR 40.0%
TGCCAATAAAGCAGTTATGG+TGG + chr5_1:67944426-67944445 Msa0745970:three_prime_UTR 40.0%
GACCACCATAACTGCTTTAT+TGG - chr5_1:67944431-67944450 None:intergenic 40.0%
ACAACAAAAAATCGGTGGTG+TGG - chr5_1:67944472-67944491 None:intergenic 40.0%
!!! CACCACCGATTTTTTGTTGT+TGG + chr5_1:67944472-67944491 Msa0745970:three_prime_UTR 40.0%
CTCCAACAACAAAAAATCGG+TGG - chr5_1:67944477-67944496 None:intergenic 40.0%
AATCGTCATATTGGCGTTCT+TGG + chr5_1:67944556-67944575 Msa0745970:three_prime_UTR 40.0%
! CCAATCATTTGGTGACACAA+TGG - chr5_1:67944585-67944604 None:intergenic 40.0%
CCATTGTGTCACCAAATGAT+TGG + chr5_1:67944582-67944601 Msa0745970:three_prime_UTR 40.0%
GGAAAACCATGCCAATCATT+TGG - chr5_1:67944596-67944615 None:intergenic 40.0%
TTCCAGCAGTATACTAGCAA+GGG - chr5_1:67944632-67944651 None:intergenic 40.0%
ATTCCAGCAGTATACTAGCA+AGG - chr5_1:67944633-67944652 None:intergenic 40.0%
CGATTCATCGCAAACCCTAA+TGG + chr5_1:67941350-67941369 Msa0745970:exon 45.0%
CTGAGAAGGTAACAGCCATT+AGG - chr5_1:67941368-67941387 None:intergenic 45.0%
CTCAGATCTTCACACAGAAG+AGG + chr5_1:67941383-67941402 Msa0745970:CDS 45.0%
TCCCTCAATGATTTCCTCTC+TGG + chr5_1:67941414-67941433 Msa0745970:CDS 45.0%
! GATGCTTTTGCCAATGTTGC+TGG + chr5_1:67941849-67941868 Msa0745970:CDS 45.0%
! CTTTTGCCAATGTTGCTGGT+TGG + chr5_1:67941853-67941872 Msa0745970:CDS 45.0%
ATCGTACCAACCAGCAACAT+TGG - chr5_1:67941862-67941881 None:intergenic 45.0%
TCTCTTCACATCTTGCTGCA+AGG + chr5_1:67941886-67941905 Msa0745970:CDS 45.0%
AGTTTCCCTGGAAAAGCTCA+AGG + chr5_1:67942039-67942058 Msa0745970:intron 45.0%
! TCACACCTTGAGCTTTTCCA+GGG - chr5_1:67942047-67942066 None:intergenic 45.0%
! TTCACACCTTGAGCTTTTCC+AGG - chr5_1:67942048-67942067 None:intergenic 45.0%
GCTCAAGGTGTGAAATTCAG+TGG + chr5_1:67942054-67942073 Msa0745970:CDS 45.0%
AGCCAAGATAGCAACATGGA+TGG + chr5_1:67942955-67942974 Msa0745970:intron 45.0%
AGAAAGGAGCTCTACAGTAC+TGG - chr5_1:67943006-67943025 None:intergenic 45.0%
TCATCATCATCTTCAGCAGC+AGG - chr5_1:67943451-67943470 None:intergenic 45.0%
CCTCTGTTTCATCACCAAAG+AGG - chr5_1:67943480-67943499 None:intergenic 45.0%
!! CCTCTTTGGTGATGAAACAG+AGG + chr5_1:67943477-67943496 Msa0745970:CDS 45.0%
!! CTTTGGTGATGAAACAGAGG+AGG + chr5_1:67943480-67943499 Msa0745970:CDS 45.0%
ATAAGAAGGCAGCAGAGGAA+AGG + chr5_1:67943503-67943522 Msa0745970:CDS 45.0%
TAAGAAGGCAGCAGAGGAAA+GGG + chr5_1:67943504-67943523 Msa0745970:CDS 45.0%
ATGTCTGTCTCATCATCCCA+TGG - chr5_1:67943723-67943742 None:intergenic 45.0%
TGAGACAGACATGAAGAAGC+TGG + chr5_1:67943731-67943750 Msa0745970:CDS 45.0%
AGACATGAAGAAGCTGGAAG+AGG + chr5_1:67943737-67943756 Msa0745970:CDS 45.0%
GAAATTCCCGGTTTAACCTG+GGG + chr5_1:67943774-67943793 Msa0745970:CDS 45.0%
! TGCATTTGTTACAGCCAAGC+TGG + chr5_1:67943903-67943922 Msa0745970:intron 45.0%
! AAGCTGGTTGCTGTTGGATA+CGG + chr5_1:67943919-67943938 Msa0745970:CDS 45.0%
AAGAAGAAACGACAGAGTGG+AGG - chr5_1:67944151-67944170 None:intergenic 45.0%
!! TGTTTGGTGTGTTTCAGTGG+TGG + chr5_1:67944174-67944193 Msa0745970:three_prime_UTR 45.0%
GCTAATCCCACAAATGTGCA+TGG - chr5_1:67944273-67944292 None:intergenic 45.0%
CAATATGACGATTGTGCCTC+AGG - chr5_1:67944549-67944568 None:intergenic 45.0%
CTGAGGCACAATCGTCATAT+TGG + chr5_1:67944547-67944566 Msa0745970:three_prime_UTR 45.0%
GTGTCACCAAATGATTGGCA+TGG + chr5_1:67944587-67944606 Msa0745970:three_prime_UTR 45.0%
!!! GATTTTTTTTTTTTTATAAA+AGG - chr5_1:67943856-67943875 None:intergenic 5.0%
TAGGGTTTGCGATGAATCGG+TGG - chr5_1:67941349-67941368 None:intergenic 50.0%
! GTTCGCAGCGTTGAAATTCC+CGG + chr5_1:67943762-67943781 Msa0745970:CDS 50.0%
ACATGCTCCCCAGGTTAAAC+CGG - chr5_1:67943784-67943803 None:intergenic 50.0%
GTATCCAACAGCAACCAGCT+TGG - chr5_1:67943920-67943939 None:intergenic 50.0%
ATGCGACAATGTCACAGCTC+TGG - chr5_1:67944047-67944066 None:intergenic 50.0%
AACGACAGAGTGGAGGAGAA+GGG - chr5_1:67944144-67944163 None:intergenic 50.0%
AAACGACAGAGTGGAGGAGA+AGG - chr5_1:67944145-67944164 None:intergenic 50.0%
AGCCCTTGCTAGTATACTGC+TGG + chr5_1:67944627-67944646 Msa0745970:three_prime_UTR 50.0%
GTAAAGTTGCGATCATGCCG+TGG + chr5_1:67944669-67944688 Msa0745970:three_prime_UTR 50.0%
GGTTTGCGATGAATCGGTGG+CGG - chr5_1:67941346-67941365 None:intergenic 55.0%
GGAGGATAAGAAGGCAGCAG+AGG + chr5_1:67943498-67943517 Msa0745970:CDS 55.0%
CATGCTCCCCAGGTTAAACC+GGG - chr5_1:67943783-67943802 None:intergenic 55.0%
ACAGCCAAGCTGGTTGCTGT+TGG + chr5_1:67943913-67943932 Msa0745970:intron 55.0%
!! AGTGGAAGTGCTCCAGCTGC+AGG + chr5_1:67942072-67942091 Msa0745970:CDS 60.0%
TTAGCAGGTGCTCCTGCAGC+TGG - chr5_1:67942087-67942106 None:intergenic 60.0%
GAAGGCAGCAGAGGAAAGGG+AGG + chr5_1:67943507-67943526 Msa0745970:CDS 60.0%
GACAGAGTGGAGGAGAAGGG+AGG - chr5_1:67944141-67944160 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 67941191 67944707 67941191 ID=Msa0745970;Name=Msa0745970
chr5_1 mRNA 67941191 67944707 67941191 ID=Msa0745970-mRNA-1;Parent=Msa0745970;Name=Msa0745970-mRNA-1;_AED=0.01;_eAED=0.01;_QI=178|1|1|1|1|1|6|627|222
chr5_1 exon 67941191 67941454 67941191 ID=Msa0745970-mRNA-1:exon:20239;Parent=Msa0745970-mRNA-1
chr5_1 exon 67941791 67941907 67941791 ID=Msa0745970-mRNA-1:exon:20240;Parent=Msa0745970-mRNA-1
chr5_1 exon 67942041 67942110 67942041 ID=Msa0745970-mRNA-1:exon:20241;Parent=Msa0745970-mRNA-1
chr5_1 exon 67943442 67943559 67943442 ID=Msa0745970-mRNA-1:exon:20242;Parent=Msa0745970-mRNA-1
chr5_1 exon 67943688 67943801 67943688 ID=Msa0745970-mRNA-1:exon:20243;Parent=Msa0745970-mRNA-1
chr5_1 exon 67943917 67944707 67943917 ID=Msa0745970-mRNA-1:exon:20244;Parent=Msa0745970-mRNA-1
chr5_1 five_prime_UTR 67941191 67941368 67941191 ID=Msa0745970-mRNA-1:five_prime_utr;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67941369 67941454 67941369 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67941791 67941907 67941791 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67942041 67942110 67942041 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67943442 67943559 67943442 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67943688 67943801 67943688 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 CDS 67943917 67944080 67943917 ID=Msa0745970-mRNA-1:cds;Parent=Msa0745970-mRNA-1
chr5_1 three_prime_UTR 67944081 67944707 67944081 ID=Msa0745970-mRNA-1:three_prime_utr;Parent=Msa0745970-mRNA-1
Gene Sequence

>Msa0745970

ATGGCTGTTACCTTCTCAGATCTTCACACAGAAGAGGGTCTCAAATCCCTCAATGATTTCCTCTCTGGAAAAAAATATATTTCTGGGGATCAATTGACAAAGGATGATATCAAAGTGTATGGATATGTTTTGGAGAAACCAAGTGATGCTTTTGCCAATGTTGCTGGTTGGTACGATGTTGTCTCTTCACATCTTGCTGCAAGTTTCCCTGGAAAAGCTCAAGGTGTGAAATTCAGTGGAAGTGCTCCAGCTGCAGGAGCACCTGCTAAAGCTGCTGCACCTGCTGCTGAAGATGATGATGATCTTGACCTCTTTGGTGATGAAACAGAGGAGGATAAGAAGGCAGCAGAGGAAAGGGAGGCATCTAAAAAGCCCGCAAAGAAGAAAGAGAGTGGCAAGTCTTCCATTCTGCTTGATGTTAAGCCATGGGATGATGAGACAGACATGAAGAAGCTGGAAGAGGCTGTTCGCAGCGTTGAAATTCCCGGTTTAACCTGGGGAGCATCCAAGCTGGTTGCTGTTGGATACGGAATCAAGAAGCTGCAGATCATGTTGACCATTATTGACGATCTTGTATCTGTAGACGACCTCATTGAAGAAAGACTCACAGTTGAGCCAATTAACGAGTATGTCCAGAGCTGTGACATTGTCGCATTCAACAAAATTTAA
Protein sequence

>Msa0745970

MAVTFSDLHTEEGLKSLNDFLSGKKYISGDQLTKDDIKVYGYVLEKPSDAFANVAGWYDVVSSHLAASFPGKAQGVKFSGSAPAAGAPAKAAAPAAEDDDDLDLFGDETEEDKKAAEEREASKKPAKKKESGKSSILLDVKPWDDETDMKKLEEAVRSVEIPGLTWGASKLVAVGYGIKKLQIMLTIIDDLVSVDDLIEERLTVEPINEYVQSCDIVAFNKI*