AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0752240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAGCAATGCCAAGGAAAT+TGG 0.216340 5_2:-5852042 Msa0752240:CDS
CCATCACTAGAATTATAAAT+TGG 0.248930 5_2:-5851743 Msa0752240:three_prime_UTR
CAAGGAAATTGGCTTCATTA+AGG 0.318093 5_2:-5852031 Msa0752240:intron
TTGATCGACTGGAGATGATA+TGG 0.329016 5_2:-5851565 Msa0752240:three_prime_UTR
TCATGGGTCACTCCAACGTT+TGG 0.416385 5_2:-5852752 Msa0752240:CDS
GAACCAGGACCATAGGTCTT+GGG 0.430692 5_2:+5852720 None:intergenic
CTTAATGAAGCCAATTTCCT+TGG 0.435363 5_2:+5852032 None:intergenic
AAACATATTTACTTCCTACT+TGG 0.442515 5_2:-5851791 Msa0752240:three_prime_UTR
ACCCATGATGATTTCTTCCT+CGG 0.445681 5_2:+5852767 None:intergenic
AGAACCAGGACCATAGGTCT+TGG 0.460388 5_2:+5852719 None:intergenic
ATTTACTTCCTACTTGGTTG+TGG 0.464265 5_2:-5851785 Msa0752240:three_prime_UTR
CCTGGTTCTCGCACATGGTA+TGG 0.483551 5_2:-5852705 Msa0752240:intron
TAGAATTATAAATTGGATGT+TGG 0.488440 5_2:-5851736 Msa0752240:three_prime_UTR
CCATACCATGTGCGAGAACC+AGG 0.488541 5_2:+5852705 None:intergenic
TTCTAGTTAAAAGCTTAGAA+AGG 0.488648 5_2:+5851665 None:intergenic
CACCCCAAGACCTATGGTCC+TGG 0.491604 5_2:-5852723 Msa0752240:CDS
TTCAATAACTAAAATCATCC+TGG 0.492527 5_2:+5851881 None:intergenic
AGTACGGACTCATGTGTTGC+AGG 0.493102 5_2:-5852074 Msa0752240:CDS
CCAATTTATAATTCTAGTGA+TGG 0.495729 5_2:+5851743 None:intergenic
AACTCTCACCCCAAGACCTA+TGG 0.505813 5_2:-5852729 Msa0752240:CDS
CCGAGCTGCAAAACGATGCC+AGG 0.511058 5_2:-5851899 Msa0752240:CDS
ACAGGTTCAAACTCAACTAC+AGG 0.533549 5_2:+5853627 None:intergenic
GATGTTGGCATCTTTGTGCA+TGG 0.537643 5_2:-5851721 Msa0752240:three_prime_UTR
ATGATTTCTTCCTCGGCGGT+AGG 0.561108 5_2:+5852774 None:intergenic
ATGGTCCTGGTTCTCGCACA+TGG 0.572066 5_2:-5852710 Msa0752240:CDS
CATGGATTGATCAGGAAGTA+CGG 0.572937 5_2:-5852090 Msa0752240:intron
TCAAACTCAACTACAGGATG+AGG 0.576270 5_2:+5853633 None:intergenic
CGCCGAGGAAGAAATCATCA+TGG 0.584765 5_2:-5852769 Msa0752240:CDS
TGTGCGAGAACCAGGACCAT+AGG 0.585453 5_2:+5852713 None:intergenic
CAATTTCCTTGGCATTGCTG+TGG 0.593734 5_2:+5852043 None:intergenic
CAACAACTCCACAACCAAGT+AGG 0.602664 5_2:+5851777 None:intergenic
AACCAGGACCATAGGTCTTG+GGG 0.607026 5_2:+5852721 None:intergenic
GTGCTTCCACAGCAATGCCA+AGG 0.621677 5_2:-5852049 Msa0752240:CDS
GCCGAGGAAGAAATCATCAT+GGG 0.630683 5_2:-5852768 Msa0752240:CDS
GGGGTGAGAGTTCCAAACGT+TGG 0.647246 5_2:+5852740 None:intergenic
CATGATGATTTCTTCCTCGG+CGG 0.651824 5_2:+5852770 None:intergenic
TCTCCAGTCGATCAAAACCG+TGG 0.689532 5_2:+5851573 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAAAAGATGATATTT+AGG - chr5_2:5852279-5852298 Msa0752240:intron 10.0%
!! AAATAAAAAGATGATATTTA+GGG - chr5_2:5852280-5852299 Msa0752240:intron 10.0%
!!! ATTTTTTTAATCTTTACAAA+TGG + chr5_2:5853585-5853604 None:intergenic 10.0%
!! TTTGTAAAGATTAAAAAAAT+AGG - chr5_2:5853585-5853604 Msa0752240:intron 10.0%
!! TTGTAAAGATTAAAAAAATA+GGG - chr5_2:5853586-5853605 Msa0752240:intron 10.0%
!! TAAATAAAAAGATGATATTT+AGG - chr5_2:5852279-5852298 Msa0752240:intron 10.0%
!! AAATAAAAAGATGATATTTA+GGG - chr5_2:5852280-5852299 Msa0752240:intron 10.0%
!!! ATTTTTTTAATCTTTACAAA+TGG + chr5_2:5853585-5853604 None:intergenic 10.0%
!! TTTGTAAAGATTAAAAAAAT+AGG - chr5_2:5853585-5853604 Msa0752240:intron 10.0%
!! TTGTAAAGATTAAAAAAATA+GGG - chr5_2:5853586-5853605 Msa0752240:intron 10.0%
!!! TTTTCAAAAGAAATGAATTT+GGG + chr5_2:5851991-5852010 None:intergenic 15.0%
!!! TTTTTCAAAAGAAATGAATT+TGG + chr5_2:5851992-5852011 None:intergenic 15.0%
!!! TTTTTTTTGGTAATATTACA+AGG - chr5_2:5852089-5852108 Msa0752240:intron 15.0%
!! AGTGTTAAAAAAAAAATGAA+AGG - chr5_2:5852247-5852266 Msa0752240:intron 15.0%
!!! TTTTCAAAAGAAATGAATTT+GGG + chr5_2:5851991-5852010 None:intergenic 15.0%
!!! TTTTTCAAAAGAAATGAATT+TGG + chr5_2:5851992-5852011 None:intergenic 15.0%
!!! TTTTTTTTGGTAATATTACA+AGG - chr5_2:5852089-5852108 Msa0752240:intron 15.0%
!! AGTGTTAAAAAAAAAATGAA+AGG - chr5_2:5852247-5852266 Msa0752240:intron 15.0%
!! AAACCAAACAAAAAATTATG+GGG + chr5_2:5851709-5851728 None:intergenic 20.0%
!! CAAACCAAACAAAAAATTAT+GGG + chr5_2:5851710-5851729 None:intergenic 20.0%
!! TCAAACCAAACAAAAAATTA+TGG + chr5_2:5851711-5851730 None:intergenic 20.0%
!! AACTATTATTATATCTCTCA+AGG - chr5_2:5851766-5851785 Msa0752240:three_prime_UTR 20.0%
!! ACTATTATTATATCTCTCAA+GGG - chr5_2:5851767-5851786 Msa0752240:three_prime_UTR 20.0%
!!! TTCATTTCTTTTGAAAAACA+AGG - chr5_2:5851994-5852013 Msa0752240:intron 20.0%
!!! TCATTTCTTTTGAAAAACAA+GGG - chr5_2:5851995-5852014 Msa0752240:intron 20.0%
!! AATTGACTGTTAGATTTATA+AGG - chr5_2:5852058-5852077 Msa0752240:CDS 20.0%
!!! TAAGGTTGTTCATTTTTTTT+TGG - chr5_2:5852076-5852095 Msa0752240:CDS 20.0%
!!! TCCTACTTTGAAAATAAAAT+GGG - chr5_2:5852795-5852814 Msa0752240:intron 20.0%
!! GTAAAATGATGTGAAAATTA+AGG + chr5_2:5852830-5852849 None:intergenic 20.0%
!! AAACGTTAAAAACAAAGAAT+GGG + chr5_2:5852871-5852890 None:intergenic 20.0%
!! TAGAATTATAAATTGGATGT+TGG - chr5_2:5853453-5853472 Msa0752240:intron 20.0%
!! AAACCAAACAAAAAATTATG+GGG + chr5_2:5851709-5851728 None:intergenic 20.0%
!! CAAACCAAACAAAAAATTAT+GGG + chr5_2:5851710-5851729 None:intergenic 20.0%
!! TCAAACCAAACAAAAAATTA+TGG + chr5_2:5851711-5851730 None:intergenic 20.0%
!! AACTATTATTATATCTCTCA+AGG - chr5_2:5851766-5851785 Msa0752240:three_prime_UTR 20.0%
!! ACTATTATTATATCTCTCAA+GGG - chr5_2:5851767-5851786 Msa0752240:three_prime_UTR 20.0%
!!! TTCATTTCTTTTGAAAAACA+AGG - chr5_2:5851994-5852013 Msa0752240:intron 20.0%
!!! TCATTTCTTTTGAAAAACAA+GGG - chr5_2:5851995-5852014 Msa0752240:intron 20.0%
!! AATTGACTGTTAGATTTATA+AGG - chr5_2:5852058-5852077 Msa0752240:CDS 20.0%
!!! TAAGGTTGTTCATTTTTTTT+TGG - chr5_2:5852076-5852095 Msa0752240:CDS 20.0%
!!! TCCTACTTTGAAAATAAAAT+GGG - chr5_2:5852795-5852814 Msa0752240:intron 20.0%
!! GTAAAATGATGTGAAAATTA+AGG + chr5_2:5852830-5852849 None:intergenic 20.0%
!! AAACGTTAAAAACAAAGAAT+GGG + chr5_2:5852871-5852890 None:intergenic 20.0%
!! TAGAATTATAAATTGGATGT+TGG - chr5_2:5853453-5853472 Msa0752240:intron 20.0%
! CTATTATTATATCTCTCAAG+GGG - chr5_2:5851768-5851787 Msa0752240:three_prime_UTR 25.0%
!!! CATTTCTTTTGAAAAACAAG+GGG - chr5_2:5851996-5852015 Msa0752240:intron 25.0%
! CAACTTACTGAAACAAATTT+CGG - chr5_2:5852643-5852662 Msa0752240:intron 25.0%
!! GTCCTACTTTGAAAATAAAA+TGG - chr5_2:5852794-5852813 Msa0752240:intron 25.0%
!!! TCCCATTTTATTTTCAAAGT+AGG + chr5_2:5852799-5852818 None:intergenic 25.0%
! CAAACGTTAAAAACAAAGAA+TGG + chr5_2:5852872-5852891 None:intergenic 25.0%
!!! TTGTTTTTAACGTTTGTACT+TGG - chr5_2:5852876-5852895 Msa0752240:intron 25.0%
! TAGTTATGATATACTAACAC+TGG + chr5_2:5852938-5852957 None:intergenic 25.0%
! TTCAATAACTAAAATCATCC+TGG + chr5_2:5853311-5853330 None:intergenic 25.0%
! AAACATATTTACTTCCTACT+TGG - chr5_2:5853398-5853417 Msa0752240:intron 25.0%
! CCAATTTATAATTCTAGTGA+TGG + chr5_2:5853449-5853468 None:intergenic 25.0%
! CCATCACTAGAATTATAAAT+TGG - chr5_2:5853446-5853465 Msa0752240:intron 25.0%
!! TTCTAGTTAAAAGCTTAGAA+AGG + chr5_2:5853527-5853546 None:intergenic 25.0%
!! ATGATATGGAACTATTTTGT+TGG - chr5_2:5853638-5853657 Msa0752240:CDS 25.0%
! CTATTATTATATCTCTCAAG+GGG - chr5_2:5851768-5851787 Msa0752240:three_prime_UTR 25.0%
!!! CATTTCTTTTGAAAAACAAG+GGG - chr5_2:5851996-5852015 Msa0752240:intron 25.0%
! CAACTTACTGAAACAAATTT+CGG - chr5_2:5852643-5852662 Msa0752240:intron 25.0%
!! GTCCTACTTTGAAAATAAAA+TGG - chr5_2:5852794-5852813 Msa0752240:intron 25.0%
!!! TCCCATTTTATTTTCAAAGT+AGG + chr5_2:5852799-5852818 None:intergenic 25.0%
! CAAACGTTAAAAACAAAGAA+TGG + chr5_2:5852872-5852891 None:intergenic 25.0%
!!! TTGTTTTTAACGTTTGTACT+TGG - chr5_2:5852876-5852895 Msa0752240:intron 25.0%
! TAGTTATGATATACTAACAC+TGG + chr5_2:5852938-5852957 None:intergenic 25.0%
! TTCAATAACTAAAATCATCC+TGG + chr5_2:5853311-5853330 None:intergenic 25.0%
! AAACATATTTACTTCCTACT+TGG - chr5_2:5853398-5853417 Msa0752240:intron 25.0%
! CCAATTTATAATTCTAGTGA+TGG + chr5_2:5853449-5853468 None:intergenic 25.0%
! CCATCACTAGAATTATAAAT+TGG - chr5_2:5853446-5853465 Msa0752240:intron 25.0%
!! TTCTAGTTAAAAGCTTAGAA+AGG + chr5_2:5853527-5853546 None:intergenic 25.0%
!! ATGATATGGAACTATTTTGT+TGG - chr5_2:5853638-5853657 Msa0752240:CDS 25.0%
!!! TCACCCCATAATTTTTTGTT+TGG - chr5_2:5851703-5851722 Msa0752240:three_prime_UTR 30.0%
!!! TTTTTTCTTCTCGAGTACAT+TGG - chr5_2:5851734-5851753 Msa0752240:three_prime_UTR 30.0%
!! TAAAACAAAGTGCTGTGAAA+TGG + chr5_2:5851799-5851818 None:intergenic 30.0%
ATAAAGTAAAAGACAACGAG+AGG + chr5_2:5851846-5851865 None:intergenic 30.0%
TGTAGACGATTATGTTAAGT+TGG - chr5_2:5851961-5851980 Msa0752240:intron 30.0%
TTTGAAAAACAAGGGGTTAT+AGG - chr5_2:5852003-5852022 Msa0752240:intron 30.0%
ATAGGTGAATTATTCTGTCT+TGG - chr5_2:5852021-5852040 Msa0752240:intron 30.0%
!! TATTTAGGGTTGAGTTTTGA+TGG - chr5_2:5852294-5852313 Msa0752240:intron 30.0%
GAACACAGTAATCAGAAATA+CGG + chr5_2:5852580-5852599 None:intergenic 30.0%
TGATGAATGACAGTAATCTA+TGG + chr5_2:5852620-5852639 None:intergenic 30.0%
! CGAAATTTGTTTCAGTAAGT+TGG + chr5_2:5852645-5852664 None:intergenic 30.0%
! CATTAAGGTGAATATTTTGC+TGG - chr5_2:5853173-5853192 Msa0752240:intron 30.0%
! TGTCTGTTTTATGATGTGAA+TGG - chr5_2:5853491-5853510 Msa0752240:intron 30.0%
!!! TCACCCCATAATTTTTTGTT+TGG - chr5_2:5851703-5851722 Msa0752240:three_prime_UTR 30.0%
!!! TTTTTTCTTCTCGAGTACAT+TGG - chr5_2:5851734-5851753 Msa0752240:three_prime_UTR 30.0%
!! TAAAACAAAGTGCTGTGAAA+TGG + chr5_2:5851799-5851818 None:intergenic 30.0%
ATAAAGTAAAAGACAACGAG+AGG + chr5_2:5851846-5851865 None:intergenic 30.0%
TGTAGACGATTATGTTAAGT+TGG - chr5_2:5851961-5851980 Msa0752240:intron 30.0%
TTTGAAAAACAAGGGGTTAT+AGG - chr5_2:5852003-5852022 Msa0752240:intron 30.0%
ATAGGTGAATTATTCTGTCT+TGG - chr5_2:5852021-5852040 Msa0752240:intron 30.0%
!! TATTTAGGGTTGAGTTTTGA+TGG - chr5_2:5852294-5852313 Msa0752240:intron 30.0%
GAACACAGTAATCAGAAATA+CGG + chr5_2:5852580-5852599 None:intergenic 30.0%
TGATGAATGACAGTAATCTA+TGG + chr5_2:5852620-5852639 None:intergenic 30.0%
! CGAAATTTGTTTCAGTAAGT+TGG + chr5_2:5852645-5852664 None:intergenic 30.0%
! CATTAAGGTGAATATTTTGC+TGG - chr5_2:5853173-5853192 Msa0752240:intron 30.0%
! TGTCTGTTTTATGATGTGAA+TGG - chr5_2:5853491-5853510 Msa0752240:intron 30.0%
!! AGACGATTATGTTAAGTTGG+TGG - chr5_2:5851964-5851983 Msa0752240:intron 35.0%
GGATCTGAATGAATTAGGAT+CGG + chr5_2:5852683-5852702 None:intergenic 35.0%
GAGTTGGATCTGAATGAATT+AGG + chr5_2:5852688-5852707 None:intergenic 35.0%
! ATTGGTTTCAAGAGTTGAGT+TGG + chr5_2:5852704-5852723 None:intergenic 35.0%
AAGCACATCGAAAAATGCAT+TGG + chr5_2:5852722-5852741 None:intergenic 35.0%
CTTAATGAAGCCAATTTCCT+TGG + chr5_2:5853160-5853179 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG - chr5_2:5853158-5853177 Msa0752240:intron 35.0%
ATTTACTTCCTACTTGGTTG+TGG - chr5_2:5853404-5853423 Msa0752240:intron 35.0%
AAAAATAGGGAGTTCTTCCA+CGG - chr5_2:5853599-5853618 Msa0752240:intron 35.0%
!! AGACGATTATGTTAAGTTGG+TGG - chr5_2:5851964-5851983 Msa0752240:intron 35.0%
GGATCTGAATGAATTAGGAT+CGG + chr5_2:5852683-5852702 None:intergenic 35.0%
GAGTTGGATCTGAATGAATT+AGG + chr5_2:5852688-5852707 None:intergenic 35.0%
! ATTGGTTTCAAGAGTTGAGT+TGG + chr5_2:5852704-5852723 None:intergenic 35.0%
AAGCACATCGAAAAATGCAT+TGG + chr5_2:5852722-5852741 None:intergenic 35.0%
CTTAATGAAGCCAATTTCCT+TGG + chr5_2:5853160-5853179 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG - chr5_2:5853158-5853177 Msa0752240:intron 35.0%
ATTTACTTCCTACTTGGTTG+TGG - chr5_2:5853404-5853423 Msa0752240:intron 35.0%
AAAAATAGGGAGTTCTTCCA+CGG - chr5_2:5853599-5853618 Msa0752240:intron 35.0%
TCAAACTCAACTACAGGATG+AGG + chr5_2:5851559-5851578 None:intergenic 40.0%
ACAGGTTCAAACTCAACTAC+AGG + chr5_2:5851565-5851584 None:intergenic 40.0%
TGAGTTTGAACCTGTGTATC+AGG - chr5_2:5851570-5851589 Msa0752240:three_prime_UTR 40.0%
TAATCATGCTCCTGATACAC+AGG + chr5_2:5851583-5851602 None:intergenic 40.0%
! TGTGTATCAGGAGCATGATT+AGG - chr5_2:5851582-5851601 Msa0752240:three_prime_UTR 40.0%
GTGAACACAAACACATCTCA+TGG + chr5_2:5851687-5851706 None:intergenic 40.0%
TGAGCTTAGCTCAGTTTGAA+AGG + chr5_2:5852153-5852172 None:intergenic 40.0%
ACCCATGATGATTTCTTCCT+CGG + chr5_2:5852425-5852444 None:intergenic 40.0%
TCGAAGAATCTTACAAGGGT+TGG + chr5_2:5852556-5852575 None:intergenic 40.0%
CGGATCGAAGAATCTTACAA+GGG + chr5_2:5852560-5852579 None:intergenic 40.0%
ACGGATCGAAGAATCTTACA+AGG + chr5_2:5852561-5852580 None:intergenic 40.0%
! TCGATGTGCTTGTACTTTTC+TGG - chr5_2:5852731-5852750 Msa0752240:CDS 40.0%
! GAAACTCTCATGGATTGATC+AGG - chr5_2:5853091-5853110 Msa0752240:intron 40.0%
! CATGGATTGATCAGGAAGTA+CGG - chr5_2:5853099-5853118 Msa0752240:intron 40.0%
CATCACTTTCCACTTCTTTC+CGG - chr5_2:5853236-5853255 Msa0752240:intron 40.0%
TTGATCGACTGGAGATGATA+TGG - chr5_2:5853624-5853643 Msa0752240:intron 40.0%
TCAAACTCAACTACAGGATG+AGG + chr5_2:5851559-5851578 None:intergenic 40.0%
ACAGGTTCAAACTCAACTAC+AGG + chr5_2:5851565-5851584 None:intergenic 40.0%
TGAGTTTGAACCTGTGTATC+AGG - chr5_2:5851570-5851589 Msa0752240:three_prime_UTR 40.0%
TAATCATGCTCCTGATACAC+AGG + chr5_2:5851583-5851602 None:intergenic 40.0%
! TGTGTATCAGGAGCATGATT+AGG - chr5_2:5851582-5851601 Msa0752240:three_prime_UTR 40.0%
GTGAACACAAACACATCTCA+TGG + chr5_2:5851687-5851706 None:intergenic 40.0%
TGAGCTTAGCTCAGTTTGAA+AGG + chr5_2:5852153-5852172 None:intergenic 40.0%
ACCCATGATGATTTCTTCCT+CGG + chr5_2:5852425-5852444 None:intergenic 40.0%
TCGAAGAATCTTACAAGGGT+TGG + chr5_2:5852556-5852575 None:intergenic 40.0%
CGGATCGAAGAATCTTACAA+GGG + chr5_2:5852560-5852579 None:intergenic 40.0%
ACGGATCGAAGAATCTTACA+AGG + chr5_2:5852561-5852580 None:intergenic 40.0%
! TCGATGTGCTTGTACTTTTC+TGG - chr5_2:5852731-5852750 Msa0752240:CDS 40.0%
! GAAACTCTCATGGATTGATC+AGG - chr5_2:5853091-5853110 Msa0752240:intron 40.0%
! CATGGATTGATCAGGAAGTA+CGG - chr5_2:5853099-5853118 Msa0752240:intron 40.0%
CATCACTTTCCACTTCTTTC+CGG - chr5_2:5853236-5853255 Msa0752240:intron 40.0%
TTGATCGACTGGAGATGATA+TGG - chr5_2:5853624-5853643 Msa0752240:intron 40.0%
AGGTTGTTATGCTCACGATG+AGG - chr5_2:5851602-5851621 Msa0752240:three_prime_UTR 45.0%
GAGTTGGATTCGTATCCTAG+TGG - chr5_2:5851660-5851679 Msa0752240:three_prime_UTR 45.0%
AACACATCTCATGGACCACT+AGG + chr5_2:5851678-5851697 None:intergenic 45.0%
CATGATGATTTCTTCCTCGG+CGG + chr5_2:5852422-5852441 None:intergenic 45.0%
GCCGAGGAAGAAATCATCAT+GGG - chr5_2:5852421-5852440 Msa0752240:intron 45.0%
CATGAGAGTTTCCACAGACA+CGG + chr5_2:5853083-5853102 None:intergenic 45.0%
CAATTTCCTTGGCATTGCTG+TGG + chr5_2:5853149-5853168 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG - chr5_2:5853147-5853166 Msa0752240:intron 45.0%
TTTCAGCGATACTGCAACAC+CGG + chr5_2:5853258-5853277 None:intergenic 45.0%
CAACAACTCCACAACCAAGT+AGG + chr5_2:5853415-5853434 None:intergenic 45.0%
GATGTTGGCATCTTTGTGCA+TGG - chr5_2:5853468-5853487 Msa0752240:intron 45.0%
AGGTTGTTATGCTCACGATG+AGG - chr5_2:5851602-5851621 Msa0752240:three_prime_UTR 45.0%
GAGTTGGATTCGTATCCTAG+TGG - chr5_2:5851660-5851679 Msa0752240:three_prime_UTR 45.0%
AACACATCTCATGGACCACT+AGG + chr5_2:5851678-5851697 None:intergenic 45.0%
CATGATGATTTCTTCCTCGG+CGG + chr5_2:5852422-5852441 None:intergenic 45.0%
GCCGAGGAAGAAATCATCAT+GGG - chr5_2:5852421-5852440 Msa0752240:intron 45.0%
CATGAGAGTTTCCACAGACA+CGG + chr5_2:5853083-5853102 None:intergenic 45.0%
CAATTTCCTTGGCATTGCTG+TGG + chr5_2:5853149-5853168 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG - chr5_2:5853147-5853166 Msa0752240:intron 45.0%
TTTCAGCGATACTGCAACAC+CGG + chr5_2:5853258-5853277 None:intergenic 45.0%
CAACAACTCCACAACCAAGT+AGG + chr5_2:5853415-5853434 None:intergenic 45.0%
GATGTTGGCATCTTTGTGCA+TGG - chr5_2:5853468-5853487 Msa0752240:intron 45.0%
!! TTTAAAAAAAAAAAAATCAA+AGG - chr5_2:5852219-5852238 Msa0752240:intron 5.0%
!! TTTAAAAAAAAAAAAATCAA+AGG - chr5_2:5852219-5852238 Msa0752240:intron 5.0%
GGATACGAATCCAACTCGAC+TGG + chr5_2:5851657-5851676 None:intergenic 50.0%
ATGATTTCTTCCTCGGCGGT+AGG + chr5_2:5852418-5852437 None:intergenic 50.0%
CGCCGAGGAAGAAATCATCA+TGG - chr5_2:5852420-5852439 Msa0752240:intron 50.0%
TCATGGGTCACTCCAACGTT+TGG - chr5_2:5852437-5852456 Msa0752240:intron 50.0%
AACTCTCACCCCAAGACCTA+TGG - chr5_2:5852460-5852479 Msa0752240:intron 50.0%
AACCAGGACCATAGGTCTTG+GGG + chr5_2:5852471-5852490 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG + chr5_2:5852472-5852491 None:intergenic 50.0%
AGAACCAGGACCATAGGTCT+TGG + chr5_2:5852473-5852492 None:intergenic 50.0%
ACAGACACGGCTACAAAACG+CGG + chr5_2:5853070-5853089 None:intergenic 50.0%
CGTGTCTGTGGAAACTCTCA+TGG - chr5_2:5853081-5853100 Msa0752240:intron 50.0%
AGTACGGACTCATGTGTTGC+AGG - chr5_2:5853115-5853134 Msa0752240:intron 50.0%
ACTGCAACACCGGAAAGAAG+TGG + chr5_2:5853248-5853267 None:intergenic 50.0%
!! CTTCCACGGTTTTGATCGAC+TGG - chr5_2:5853613-5853632 Msa0752240:intron 50.0%
TCTCCAGTCGATCAAAACCG+TGG + chr5_2:5853619-5853638 None:intergenic 50.0%
GGATACGAATCCAACTCGAC+TGG + chr5_2:5851657-5851676 None:intergenic 50.0%
ATGATTTCTTCCTCGGCGGT+AGG + chr5_2:5852418-5852437 None:intergenic 50.0%
CGCCGAGGAAGAAATCATCA+TGG - chr5_2:5852420-5852439 Msa0752240:intron 50.0%
TCATGGGTCACTCCAACGTT+TGG - chr5_2:5852437-5852456 Msa0752240:intron 50.0%
AACTCTCACCCCAAGACCTA+TGG - chr5_2:5852460-5852479 Msa0752240:intron 50.0%
AACCAGGACCATAGGTCTTG+GGG + chr5_2:5852471-5852490 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG + chr5_2:5852472-5852491 None:intergenic 50.0%
AGAACCAGGACCATAGGTCT+TGG + chr5_2:5852473-5852492 None:intergenic 50.0%
ACAGACACGGCTACAAAACG+CGG + chr5_2:5853070-5853089 None:intergenic 50.0%
CGTGTCTGTGGAAACTCTCA+TGG - chr5_2:5853081-5853100 Msa0752240:intron 50.0%
AGTACGGACTCATGTGTTGC+AGG - chr5_2:5853115-5853134 Msa0752240:intron 50.0%
ACTGCAACACCGGAAAGAAG+TGG + chr5_2:5853248-5853267 None:intergenic 50.0%
!! CTTCCACGGTTTTGATCGAC+TGG - chr5_2:5853613-5853632 Msa0752240:intron 50.0%
TCTCCAGTCGATCAAAACCG+TGG + chr5_2:5853619-5853638 None:intergenic 50.0%
TCCTTCTCCTCCTCAGCATC+CGG + chr5_2:5851633-5851652 None:intergenic 55.0%
GGGGTGAGAGTTCCAAACGT+TGG + chr5_2:5852452-5852471 None:intergenic 55.0%
TGTGCGAGAACCAGGACCAT+AGG + chr5_2:5852479-5852498 None:intergenic 55.0%
! ATGGTCCTGGTTCTCGCACA+TGG - chr5_2:5852479-5852498 Msa0752240:intron 55.0%
CCATACCATGTGCGAGAACC+AGG + chr5_2:5852487-5852506 None:intergenic 55.0%
! CCTGGTTCTCGCACATGGTA+TGG - chr5_2:5852484-5852503 Msa0752240:intron 55.0%
! GCGTTTTGTAGCCGTGTCTG+TGG - chr5_2:5853069-5853088 Msa0752240:intron 55.0%
GTGCTTCCACAGCAATGCCA+AGG - chr5_2:5853140-5853159 Msa0752240:intron 55.0%
! GCATCGTTTTGCAGCTCGGA+AGG + chr5_2:5853289-5853308 None:intergenic 55.0%
! CCTGGCATCGTTTTGCAGCT+CGG + chr5_2:5853293-5853312 None:intergenic 55.0%
TCCTTCTCCTCCTCAGCATC+CGG + chr5_2:5851633-5851652 None:intergenic 55.0%
GGGGTGAGAGTTCCAAACGT+TGG + chr5_2:5852452-5852471 None:intergenic 55.0%
TGTGCGAGAACCAGGACCAT+AGG + chr5_2:5852479-5852498 None:intergenic 55.0%
! ATGGTCCTGGTTCTCGCACA+TGG - chr5_2:5852479-5852498 Msa0752240:intron 55.0%
CCATACCATGTGCGAGAACC+AGG + chr5_2:5852487-5852506 None:intergenic 55.0%
! CCTGGTTCTCGCACATGGTA+TGG - chr5_2:5852484-5852503 Msa0752240:intron 55.0%
! GCGTTTTGTAGCCGTGTCTG+TGG - chr5_2:5853069-5853088 Msa0752240:intron 55.0%
GTGCTTCCACAGCAATGCCA+AGG - chr5_2:5853140-5853159 Msa0752240:intron 55.0%
! GCATCGTTTTGCAGCTCGGA+AGG + chr5_2:5853289-5853308 None:intergenic 55.0%
! CCTGGCATCGTTTTGCAGCT+CGG + chr5_2:5853293-5853312 None:intergenic 55.0%
TGCTCACGATGAGGTCCACC+CGG - chr5_2:5851611-5851630 Msa0752240:three_prime_UTR 60.0%
CCTTCTCCTCCTCAGCATCC+GGG + chr5_2:5851632-5851651 None:intergenic 60.0%
GGAGAAGGAGCCAGTCGAGT+TGG - chr5_2:5851644-5851663 Msa0752240:three_prime_UTR 60.0%
TGCTGCTGCTGCTGCTGCTA+GGG - chr5_2:5852379-5852398 Msa0752240:intron 60.0%
CACCCCAAGACCTATGGTCC+TGG - chr5_2:5852466-5852485 Msa0752240:intron 60.0%
CCGAGCTGCAAAACGATGCC+AGG - chr5_2:5853290-5853309 Msa0752240:intron 60.0%
TGCTCACGATGAGGTCCACC+CGG - chr5_2:5851611-5851630 Msa0752240:three_prime_UTR 60.0%
CCTTCTCCTCCTCAGCATCC+GGG + chr5_2:5851632-5851651 None:intergenic 60.0%
GGAGAAGGAGCCAGTCGAGT+TGG - chr5_2:5851644-5851663 Msa0752240:three_prime_UTR 60.0%
TGCTGCTGCTGCTGCTGCTA+GGG - chr5_2:5852379-5852398 Msa0752240:intron 60.0%
CACCCCAAGACCTATGGTCC+TGG - chr5_2:5852466-5852485 Msa0752240:intron 60.0%
CCGAGCTGCAAAACGATGCC+AGG - chr5_2:5853290-5853309 Msa0752240:intron 60.0%
TGAGGTCCACCCGGATGCTG+AGG - chr5_2:5851620-5851639 Msa0752240:three_prime_UTR 65.0%
TCTCCTCCTCAGCATCCGGG+TGG + chr5_2:5851629-5851648 None:intergenic 65.0%
CCCGGATGCTGAGGAGGAGA+AGG - chr5_2:5851629-5851648 Msa0752240:three_prime_UTR 65.0%
CTGCTGCTGCTGCTGCTGCT+AGG - chr5_2:5852378-5852397 Msa0752240:intron 65.0%
TGAGGTCCACCCGGATGCTG+AGG - chr5_2:5851620-5851639 Msa0752240:three_prime_UTR 65.0%
TCTCCTCCTCAGCATCCGGG+TGG + chr5_2:5851629-5851648 None:intergenic 65.0%
CCCGGATGCTGAGGAGGAGA+AGG - chr5_2:5851629-5851648 Msa0752240:three_prime_UTR 65.0%
CTGCTGCTGCTGCTGCTGCT+AGG - chr5_2:5852378-5852397 Msa0752240:intron 65.0%
GGTCCACCCGGATGCTGAGG+AGG - chr5_2:5851623-5851642 Msa0752240:three_prime_UTR 70.0%
GAGATCGCTGCCTACCGCCG+AGG - chr5_2:5852405-5852424 Msa0752240:intron 70.0%
GGTCCACCCGGATGCTGAGG+AGG - chr5_2:5851623-5851642 Msa0752240:three_prime_UTR 70.0%
GAGATCGCTGCCTACCGCCG+AGG - chr5_2:5852405-5852424 Msa0752240:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 5851532 5853679 5851532 ID=Msa0752240;Name=Msa0752240
chr5_2 mRNA 5851532 5853679 5851532 ID=Msa0752240-mRNA-1;Parent=Msa0752240;Name=Msa0752240-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.33|0.25|0.75|1|1|4|331|101
chr5_2 exon 5851532 5851944 5851532 ID=Msa0752240-mRNA-1:exon:2455;Parent=Msa0752240-mRNA-1
chr5_2 exon 5852032 5852098 5852032 ID=Msa0752240-mRNA-1:exon:2454;Parent=Msa0752240-mRNA-1
chr5_2 exon 5852706 5852811 5852706 ID=Msa0752240-mRNA-1:exon:2453;Parent=Msa0752240-mRNA-1
chr5_2 exon 5853629 5853679 5853629 ID=Msa0752240-mRNA-1:exon:2452;Parent=Msa0752240-mRNA-1
chr5_2 CDS 5853629 5853679 5853629 ID=Msa0752240-mRNA-1:cds;Parent=Msa0752240-mRNA-1
chr5_2 CDS 5852706 5852811 5852706 ID=Msa0752240-mRNA-1:cds;Parent=Msa0752240-mRNA-1
chr5_2 CDS 5852032 5852098 5852032 ID=Msa0752240-mRNA-1:cds;Parent=Msa0752240-mRNA-1
chr5_2 CDS 5851863 5851944 5851863 ID=Msa0752240-mRNA-1:cds;Parent=Msa0752240-mRNA-1
chr5_2 three_prime_UTR 5851532 5851862 5851532 ID=Msa0752240-mRNA-1:three_prime_utr;Parent=Msa0752240-mRNA-1
Gene Sequence

>Msa0752240

ATGAGTGATACTCATGTGCATGAGCCTCATCCTGTAGTTGAGTTTGAACCTGGTTTGAGATCGCTGCCTACCGCCGAGGAAGAAATCATCATGGGTCACTCCAACGTTTGGAACTCTCACCCCAAGACCTATGGTCCTGGTTCTCGCACATGGTATGGAAGTACGGACTCATGTGTTGCAGGCAGTGCTTCCACAGCAATGCCAAGGAAATTGGCTTCATTAAGTATCGCTGAAAAGTTGAGTCCTTCCGAGCTGCAAAACGATGCCAGGATGATTTTAGTTATTGAAGAACTTATAATTTTGTAG
Protein sequence

>Msa0752240

MSDTHVHEPHPVVEFEPGLRSLPTAEEEIIMGHSNVWNSHPKTYGPGSRTWYGSTDSCVAGSASTAMPRKLASLSIAEKLSPSELQNDARMILVIEELIIL*