AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0108750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108750 MtrunA17_Chr1g0161301 99.639 277 1 0 1 277 1 277 0.0 558
Msa0108750 MtrunA17_Chr8g0391761 98.182 165 3 0 113 277 20 184 7.19e-117 333
Msa0108750 MtrunA17_Chr4g0047041 32.727 165 94 4 89 244 147 303 1.82e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108750 AT3G57490.1 92.308 221 17 0 43 263 41 261 2.40e-152 426
Msa0108750 AT2G41840.1 90.308 227 19 1 42 268 49 272 4.85e-151 424
Msa0108750 AT1G58684.1 87.712 236 26 1 43 275 49 284 2.79e-150 422
Msa0108750 AT1G59359.1 87.712 236 26 1 43 275 49 284 2.79e-150 422
Msa0108750 AT1G58983.1 87.712 236 26 1 43 275 49 284 2.79e-150 422
Msa0108750 AT1G58380.1 87.342 237 27 1 42 275 48 284 3.51e-150 421
Msa0108750 AT2G33800.1 29.530 149 97 2 89 237 147 287 2.37e-14 72.0

Find 106 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCTCTCTCTGCATTTCT+AGG 0.161553 1_3:-20381180 Msa0108750:five_prime_UTR
GTTTCTTGACACCTGAATTC+TGG 0.269557 1_3:-20378950 Msa0108750:CDS
GCAAACTGCAAAACCTTCTT+AGG 0.278482 1_3:+20380514 None:intergenic
AACGAAATTGCCAAGGGTTT+TGG 0.290170 1_3:+20380453 None:intergenic
TAATGTATGTTAAGCATATT+AGG 0.297570 1_3:-20378797 Msa0108750:three_prime_UTR
GCCGGATTCGGAAGAGGTTT+CGG 0.325361 1_3:-20381072 Msa0108750:CDS
GTATCTTTCTTTATTGCGTT+TGG 0.327562 1_3:-20378714 Msa0108750:three_prime_UTR
GACTGTCTGATGAAGACTTA+TGG 0.329475 1_3:-20378972 Msa0108750:CDS
CCGGGTTCGTTGACCGGCTC+TGG 0.332475 1_3:+20380795 None:intergenic
CGAGTCTCCTTCCAGAATTC+AGG 0.345000 1_3:+20378939 None:intergenic
GATGATTTGGTGTTCTTTGA+TGG 0.347373 1_3:+20380882 None:intergenic
TCCGAAACCTCTTCCGAATC+CGG 0.356237 1_3:+20381071 None:intergenic
GTTCCTGCACCGAGAGGTTC+TGG 0.361388 1_3:-20380556 Msa0108750:CDS
CGACTAAGGTGTCGATGATT+TGG 0.372123 1_3:+20380869 None:intergenic
CCAACATATCAGTGTACTCT+TGG 0.379970 1_3:+20378898 None:intergenic
TTATCCAATAAAAGCAAAAC+TGG 0.380330 1_3:+20378817 None:intergenic
CACAACAAAGGCCTTGATCT+TGG 0.385331 1_3:-20378868 Msa0108750:CDS
TTCTGGTATTGTTGCTGCTA+GGG 0.392766 1_3:-20380539 Msa0108750:CDS
ACTGTGCCGTGTAAGGTTAC+TGG 0.397057 1_3:-20380607 Msa0108750:CDS
AAGATCAAGGCCTTTGTTGT+GGG 0.399949 1_3:+20378870 None:intergenic
AGACGGCCGAGCTTTGTTAC+TGG 0.409258 1_3:+20380958 None:intergenic
AACCCTTGGCAATTTCGTTA+AGG 0.410713 1_3:-20380449 Msa0108750:intron
CAAGATCAAGGCCTTTGTTG+TGG 0.418524 1_3:+20378869 None:intergenic
TGCTGCTAGGGTTCCTAAGA+AGG 0.419749 1_3:-20380527 Msa0108750:CDS
TTCAAAGCCTTTGTTGTTGT+GGG 0.422150 1_3:-20380772 Msa0108750:CDS
TTTGAACCGGGTTCGTTGAC+CGG 0.422499 1_3:+20380789 None:intergenic
CAACAACAAAGGCTTTGAAC+CGG 0.423689 1_3:+20380776 None:intergenic
TTCCTGTGAGAAGAGGTTAC+TGG 0.425027 1_3:-20380654 Msa0108750:CDS
TACCTCTCTCTGCATTTCTA+GGG 0.425343 1_3:-20381179 Msa0108750:five_prime_UTR
GGAGGTCGCCGCAGAGGCGC+TGG 0.430160 1_3:-20381009 Msa0108750:CDS
GCTCCTCGGTCTGCCATTGT+TGG 0.432890 1_3:+20381108 None:intergenic
ACCGGAGACAGAGCCGGATT+CGG 0.440482 1_3:-20381084 Msa0108750:CDS
TCCGAATCCGGCTCTGTCTC+CGG 0.446226 1_3:+20381083 None:intergenic
CTCTGTCTCCGGTAGCTCCT+CGG 0.448842 1_3:+20381094 None:intergenic
GCTCTGGTTTGTTTCTGAAC+CGG 0.455324 1_3:+20380811 None:intergenic
ATAAATCAAGTAGAAAACTA+TGG 0.455426 1_3:+20378636 None:intergenic
GTTAGGTGTGAAGTGTAGTA+AGG 0.467876 1_3:-20380728 Msa0108750:CDS
GACGGCCGAGCTTTGTTACT+GGG 0.470512 1_3:+20380959 None:intergenic
GTTCTGGTATTGTTGCTGCT+AGG 0.471297 1_3:-20380540 Msa0108750:CDS
CTTGATCTTGGAGGAGGAAA+GGG 0.473493 1_3:-20378856 Msa0108750:CDS
TCCTGTGAGAAGAGGTTACT+GGG 0.474658 1_3:-20380653 Msa0108750:CDS
GCCACATACTGTGCCGTGTA+AGG 0.481615 1_3:-20380614 Msa0108750:CDS
TTTCAGCGTGGGACCGACTA+AGG 0.481861 1_3:+20380855 None:intergenic
GTTCAAAGCCTTTGTTGTTG+TGG 0.489519 1_3:-20380773 Msa0108750:CDS
GACAGAGCCGGATTCGGAAG+AGG 0.501973 1_3:-20381078 Msa0108750:CDS
CTCGGCCGTCTCGTCAAAGA+AGG 0.518865 1_3:-20380946 Msa0108750:CDS
AACAACAAAGGCTTTGAACC+GGG 0.520283 1_3:+20380777 None:intergenic
ATCACTTCGTCTTTCAGCGT+GGG 0.523052 1_3:+20380844 None:intergenic
CCTTTCCTCCTCCAAGATCA+AGG 0.523177 1_3:+20378857 None:intergenic
AGAGGCGGAGATCGTGGCCG+TGG 0.527803 1_3:-20381030 Msa0108750:CDS
ACCTTACACGGCACAGTATG+TGG 0.528460 1_3:+20380613 None:intergenic
TGGTTCTGTTACTGTTCGTA+TGG 0.533362 1_3:-20380578 Msa0108750:CDS
CCAAGAGTACACTGATATGT+TGG 0.536690 1_3:-20378898 Msa0108750:CDS
CGTGGTCGAGGTGGCAGAGG+CGG 0.538891 1_3:-20381045 Msa0108750:CDS
CACTTTCCAGTAACCTTACA+CGG 0.538940 1_3:+20380601 None:intergenic
ATACCAGAACCTCTCGGTGC+AGG 0.541543 1_3:+20380553 None:intergenic
ATGATTTGGTGTTCTTTGAT+GGG 0.541728 1_3:+20380883 None:intergenic
ATGGCAGACCGAGGAGCTAC+CGG 0.543216 1_3:-20381102 Msa0108750:CDS
AGGCGCTGGCCGTCGTGAAG+AGG 0.545371 1_3:-20380995 Msa0108750:CDS
CACTCTCTCTCAACCAACAA+TGG 0.546279 1_3:-20381121 Msa0108750:exon
CGCCTCTGCGGCGACCTCCA+CGG 0.546686 1_3:+20381013 None:intergenic
GGTTTCGGAGATCGTGGTCG+AGG 0.546990 1_3:-20381057 Msa0108750:CDS
CTTGACACCTGAATTCTGGA+AGG 0.547375 1_3:-20378946 Msa0108750:CDS
CCTTGATCTTGGAGGAGGAA+AGG 0.547470 1_3:-20378857 Msa0108750:CDS
AACAAAGGCCTTGATCTTGG+AGG 0.548080 1_3:-20378865 Msa0108750:CDS
CATCACTTCGTCTTTCAGCG+TGG 0.548494 1_3:+20380843 None:intergenic
GGTTGAGAGAGAGTGAGAGA+AGG 0.549893 1_3:+20381129 None:intergenic
GATCGTGGTCGAGGTGGCAG+AGG 0.555874 1_3:-20381048 Msa0108750:CDS
ATATCAGTGTACTCTTGGAA+TGG 0.556129 1_3:+20378903 None:intergenic
CCAGAGCCGGTCAACGAACC+CGG 0.560802 1_3:-20380795 Msa0108750:CDS
AGGGGATCTACCAAAACCCT+TGG 0.565634 1_3:-20380463 Msa0108750:CDS
GGAGCTACCGGAGACAGAGC+CGG 0.566711 1_3:-20381090 Msa0108750:CDS
AAAGGCCTTGATCTTGGAGG+AGG 0.570185 1_3:-20378862 Msa0108750:CDS
CCCCAGTAACCTCTTCTCAC+AGG 0.570541 1_3:+20380652 None:intergenic
AACAATGGTCATGTTGGGTT+AGG 0.571470 1_3:-20380745 Msa0108750:CDS
GAAGTTGCTACTGCAATTCG+CGG 0.571598 1_3:-20380706 Msa0108750:CDS
TGGGTCCCAGTAACAAAGCT+CGG 0.574303 1_3:-20380964 Msa0108750:CDS
GTGACAACAATGGTCATGTT+GGG 0.588102 1_3:-20380750 Msa0108750:CDS
GGTGACAACAATGGTCATGT+TGG 0.588840 1_3:-20380751 Msa0108750:CDS
GGTGGCAGAGGCGGAGATCG+TGG 0.589603 1_3:-20381036 Msa0108750:CDS
GGCCGTGGAGGTCGCCGCAG+AGG 0.598782 1_3:-20381015 Msa0108750:CDS
GTTCTTTGATGGGAAGAGAG+TGG 0.601081 1_3:+20380893 None:intergenic
AGACCTTAACGAAATTGCCA+AGG 0.604222 1_3:+20380446 None:intergenic
GTTGTCACCCACAACAACAA+AGG 0.604712 1_3:+20380765 None:intergenic
GGTAGATTTGTTCTAAGCTG+CGG 0.604842 1_3:+20380917 None:intergenic
CTTTGATGGGAAGAGAGTGG+AGG 0.605151 1_3:+20380896 None:intergenic
CGAAGTGATGAAAATCATGC+CGG 0.605193 1_3:-20380830 Msa0108750:CDS
GTTCAGAAACAAACCAGAGC+CGG 0.607188 1_3:-20380808 Msa0108750:CDS
GTTGGCAAAGCCCACAACAA+AGG 0.608366 1_3:-20378880 Msa0108750:CDS
GACCTTAACGAAATTGCCAA+GGG 0.610712 1_3:+20380447 None:intergenic
CACGACGGCCAGCGCCTCTG+CGG 0.615990 1_3:+20381001 None:intergenic
GGAAGAGGTTTCGGAGATCG+TGG 0.617372 1_3:-20381063 Msa0108750:CDS
GTTGTTGTGGGTGACAACAA+TGG 0.621205 1_3:-20380760 Msa0108750:CDS
TTCGGAGATCGTGGTCGAGG+TGG 0.626828 1_3:-20381054 Msa0108750:CDS
AGTGTGATTCCTGTGAGAAG+AGG 0.634613 1_3:-20380661 Msa0108750:CDS
AGAGTGAGAGAAGGAGAATG+CGG 0.636811 1_3:+20381138 None:intergenic
AACCCTAGAAATGCAGAGAG+AGG 0.638699 1_3:+20381177 None:intergenic
TGTAAGGTTACTGGAAAGTG+TGG 0.643088 1_3:-20380598 Msa0108750:CDS
GCAACAATACCAGAACCTCT+CGG 0.646729 1_3:+20380547 None:intergenic
CAAATCATCGACACCTTAGT+CGG 0.654265 1_3:-20380868 Msa0108750:CDS
CGTATGGTTCCTGCACCGAG+AGG 0.664454 1_3:-20380562 Msa0108750:CDS
GGCGGAGATCGTGGCCGTGG+AGG 0.667002 1_3:-20381027 Msa0108750:CDS
GATCTTGGAGGAGGAAAGGG+CGG 0.671501 1_3:-20378853 Msa0108750:CDS
CTGTGAGAAGAGGTTACTGG+GGG 0.685323 1_3:-20380651 Msa0108750:CDS
CAACCAACAATGGCAGACCG+AGG 0.687866 1_3:-20381111 Msa0108750:exon
CCTGTGAGAAGAGGTTACTG+GGG 0.696718 1_3:-20380652 Msa0108750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAAATTAAAATATGA+AGG + chr1_3:20379420-20379439 None:intergenic 10.0%
!! AATAAATGAATATATAAAAC+AGG + chr1_3:20379579-20379598 None:intergenic 10.0%
!!! TATTTCAATATTTTTTTTGA+AGG - chr1_3:20380261-20380280 Msa0108750:intron 10.0%
!!! TATATAATTGAATGAATTTT+TGG - chr1_3:20381156-20381175 Msa0108750:five_prime_UTR 10.0%
!! AATTAATCATTTGCAAAATT+TGG - chr1_3:20379874-20379893 Msa0108750:intron 15.0%
!!! ATTGATTTGAAAATAAATGA+TGG - chr1_3:20380235-20380254 Msa0108750:intron 15.0%
!! TTCAATTGTATATTATAAAC+AGG - chr1_3:20380533-20380552 Msa0108750:CDS 15.0%
!! TCAATTGTATATTATAAACA+GGG - chr1_3:20380534-20380553 Msa0108750:CDS 15.0%
!!! TTTATTTGAGTTGTATAATG+TGG - chr1_3:20379593-20379612 Msa0108750:intron 20.0%
!! AATAGATAGATAAAAACAAG+TGG + chr1_3:20380046-20380065 None:intergenic 20.0%
!! TCTTATTGTGAGTAAAATTT+AGG - chr1_3:20380190-20380209 Msa0108750:intron 20.0%
!!! ATCTTTAATTTGTTCATCAA+TGG - chr1_3:20380336-20380355 Msa0108750:intron 20.0%
!!! ATCCATTTAAAATGTAAAGT+GGG + chr1_3:20380370-20380389 None:intergenic 20.0%
!!! AATCCATTTAAAATGTAAAG+TGG + chr1_3:20380371-20380390 None:intergenic 20.0%
!!! TCATAGAATTTAACTTGAAA+TGG - chr1_3:20380413-20380432 Msa0108750:intron 20.0%
!! CAATTGTATATTATAAACAG+GGG - chr1_3:20380535-20380554 Msa0108750:CDS 20.0%
!! ATTTCAAAATATCATTCCAA+GGG + chr1_3:20380561-20380580 None:intergenic 20.0%
!! AATTTCAAAATATCATTCCA+AGG + chr1_3:20380562-20380581 None:intergenic 20.0%
!! CATTTGTTTGTATAATCAAA+TGG - chr1_3:20380613-20380632 Msa0108750:CDS 20.0%
!! CAACTAATAAAGTTTATATG+CGG + chr1_3:20380656-20380675 None:intergenic 20.0%
!!! TTAATGCTATTTATTTTTGC+AGG - chr1_3:20380825-20380844 Msa0108750:CDS 20.0%
!! TAATGTATGTTAAGCATATT+AGG - chr1_3:20381031-20381050 Msa0108750:CDS 20.0%
!!! ATGATGTTTTTACTTCTTCA+AGG - chr1_3:20379345-20379364 Msa0108750:intron 25.0%
!!! TGATGTTTTTACTTCTTCAA+GGG - chr1_3:20379346-20379365 Msa0108750:intron 25.0%
! GTAAATAGAAGCTAGATAAA+TGG - chr1_3:20380081-20380100 Msa0108750:intron 25.0%
!! TACCCACTTTACATTTTAAA+TGG - chr1_3:20380365-20380384 Msa0108750:intron 25.0%
! ATTATAATCAACAGACTTGT+TGG - chr1_3:20380470-20380489 Msa0108750:CDS 25.0%
!! TTATAATCAACAGACTTGTT+GGG - chr1_3:20380471-20380490 Msa0108750:CDS 25.0%
! TTTAATGTTCTTAACCACAA+GGG + chr1_3:20380589-20380608 None:intergenic 25.0%
!! TTTTAATGTTCTTAACCACA+AGG + chr1_3:20380590-20380609 None:intergenic 25.0%
!!! TTTACCAGTTTTGCTTTTAT+TGG - chr1_3:20381007-20381026 Msa0108750:CDS 25.0%
! TTATCCAATAAAAGCAAAAC+TGG + chr1_3:20381014-20381033 None:intergenic 25.0%
!! ATGATTTGGTGTTCTTTGAT+GGG + chr1_3:20378948-20378967 None:intergenic 30.0%
!! GATGTTTTTACTTCTTCAAG+GGG - chr1_3:20379347-20379366 Msa0108750:intron 30.0%
GATAAGTTGTTTATCCAAAC+AGG + chr1_3:20379620-20379639 None:intergenic 30.0%
!! GCTGTTTTCATAAGCTATAT+TGG - chr1_3:20379685-20379704 Msa0108750:intron 30.0%
AAGCTATATTGGAGAACTTA+TGG - chr1_3:20379696-20379715 Msa0108750:intron 30.0%
CTATCAATTTACGAGATAGA+TGG - chr1_3:20379956-20379975 Msa0108750:intron 30.0%
!! TAGTGACTTCGCTATTTTAA+AGG + chr1_3:20380006-20380025 None:intergenic 30.0%
TAAAATAGCGAAGTCACTAA+TGG - chr1_3:20380007-20380026 Msa0108750:intron 30.0%
AAAATAGCGAAGTCACTAAT+GGG - chr1_3:20380008-20380027 Msa0108750:intron 30.0%
AAAAACAAGTGGAAGATTAG+AGG + chr1_3:20380035-20380054 None:intergenic 30.0%
ATCTATCTATTTCCCTTGAA+TGG - chr1_3:20380055-20380074 Msa0108750:intron 30.0%
TCTATTTACTGTCCATTCAA+GGG + chr1_3:20380070-20380089 None:intergenic 30.0%
TTCTATTTACTGTCCATTCA+AGG + chr1_3:20380071-20380090 None:intergenic 30.0%
!!! ATATTTTTTTTGAAGGAGCC+TGG - chr1_3:20380268-20380287 Msa0108750:intron 30.0%
CCAATCATATTGAAATATCC+AGG + chr1_3:20380289-20380308 None:intergenic 30.0%
CCTGGATATTTCAATATGAT+TGG - chr1_3:20380286-20380305 Msa0108750:intron 30.0%
!! TTTGTGTTGGTTTGTTTCAT+TGG - chr1_3:20380706-20380725 Msa0108750:CDS 30.0%
!! GTTTCATTGGTTTATAGTTC+TGG - chr1_3:20380719-20380738 Msa0108750:CDS 30.0%
!!! ATTCTTTTTTGTTGCTACTG+TGG - chr1_3:20381067-20381086 Msa0108750:CDS 30.0%
! GTGGTTTACTTTCTTTTTGA+AGG - chr1_3:20381086-20381105 Msa0108750:CDS 30.0%
GTATCTTTCTTTATTGCGTT+TGG - chr1_3:20381114-20381133 Msa0108750:exon 30.0%
! ATTTTTCTTCCTCTTCACGA+CGG + chr1_3:20378845-20378864 None:intergenic 35.0%
TCGTGAAGAGGAAGAAAAAT+GGG - chr1_3:20378845-20378864 Msa0108750:CDS 35.0%
!! GATGATTTGGTGTTCTTTGA+TGG + chr1_3:20378949-20378968 None:intergenic 35.0%
!! TTCAAAGCCTTTGTTGTTGT+GGG - chr1_3:20379056-20379075 Msa0108750:intron 35.0%
TTGTATAATGTGGACCTGTT+TGG - chr1_3:20379603-20379622 Msa0108750:intron 35.0%
TGGTGTGATTATATGTAGCT+AGG - chr1_3:20379894-20379913 Msa0108750:intron 35.0%
GATAGATGGACATAAGTGAT+AGG - chr1_3:20379970-20379989 Msa0108750:intron 35.0%
GACATAAGTGATAGGTTAGA+GGG - chr1_3:20379978-20379997 Msa0108750:intron 35.0%
! CATCATTTGAAAGCATGTCT+AGG + chr1_3:20380123-20380142 None:intergenic 35.0%
CATGCTTTCAAATGATGTGT+TGG - chr1_3:20380126-20380145 Msa0108750:intron 35.0%
!! ATGATGTGTTGGTGCTAAAT+AGG - chr1_3:20380137-20380156 Msa0108750:intron 35.0%
TATGTGTGTACAAACTTGAG+TGG - chr1_3:20380163-20380182 Msa0108750:intron 35.0%
!!! TTTTGAAATTGACTCCCTTG+TGG - chr1_3:20380572-20380591 Msa0108750:CDS 35.0%
CAAACACGAGTAACAAATTC+TGG + chr1_3:20380777-20380796 None:intergenic 35.0%
ATATCAGTGTACTCTTGGAA+TGG + chr1_3:20380928-20380947 None:intergenic 35.0%
GTCGTGAAGAGGAAGAAAAA+TGG - chr1_3:20378844-20378863 Msa0108750:exon 40.0%
! TTTTGCCTTCTTTGACGAGA+CGG + chr1_3:20378890-20378909 None:intergenic 40.0%
! GGTAGATTTGTTCTAAGCTG+CGG + chr1_3:20378914-20378933 None:intergenic 40.0%
CAAATCATCGACACCTTAGT+CGG - chr1_3:20378960-20378979 Msa0108750:CDS 40.0%
CGAAGTGATGAAAATCATGC+CGG - chr1_3:20378998-20379017 Msa0108750:intron 40.0%
!! AACAACAAAGGCTTTGAACC+GGG + chr1_3:20379054-20379073 None:intergenic 40.0%
!! CAACAACAAAGGCTTTGAAC+CGG + chr1_3:20379055-20379074 None:intergenic 40.0%
!! GTTCAAAGCCTTTGTTGTTG+TGG - chr1_3:20379055-20379074 Msa0108750:intron 40.0%
GTGACAACAATGGTCATGTT+GGG - chr1_3:20379078-20379097 Msa0108750:intron 40.0%
AACAATGGTCATGTTGGGTT+AGG - chr1_3:20379083-20379102 Msa0108750:intron 40.0%
GTTAGGTGTGAAGTGTAGTA+AGG - chr1_3:20379100-20379119 Msa0108750:intron 40.0%
GTTACTGGGGGAATAAGATT+GGG - chr1_3:20379189-20379208 Msa0108750:intron 40.0%
CACTTTCCAGTAACCTTACA+CGG + chr1_3:20379230-20379249 None:intergenic 40.0%
TGTAAGGTTACTGGAAAGTG+TGG - chr1_3:20379230-20379249 Msa0108750:intron 40.0%
! TGGTTCTGTTACTGTTCGTA+TGG - chr1_3:20379250-20379269 Msa0108750:intron 40.0%
TTCTGGTATTGTTGCTGCTA+GGG - chr1_3:20379289-20379308 Msa0108750:intron 40.0%
GCAAACTGCAAAACCTTCTT+AGG + chr1_3:20379317-20379336 None:intergenic 40.0%
! AAGAAGGTTTTGCAGTTTGC+TGG - chr1_3:20379317-20379336 Msa0108750:intron 40.0%
! AACGAAATTGCCAAGGGTTT+TGG + chr1_3:20379378-20379397 None:intergenic 40.0%
AACCCTTGGCAATTTCGTTA+AGG - chr1_3:20379379-20379398 Msa0108750:intron 40.0%
GACCTTAACGAAATTGCCAA+GGG + chr1_3:20379384-20379403 None:intergenic 40.0%
AGACCTTAACGAAATTGCCA+AGG + chr1_3:20379385-20379404 None:intergenic 40.0%
CATACAGGGTCTATTGTGAT+TGG + chr1_3:20379824-20379843 None:intergenic 40.0%
GGTTAGTGACAGTGTTAGTT+TGG - chr1_3:20379915-20379934 Msa0108750:intron 40.0%
GGACATAAGTGATAGGTTAG+AGG - chr1_3:20379977-20379996 Msa0108750:intron 40.0%
ATATTATAAACAGGGGCCCT+TGG - chr1_3:20380542-20380561 Msa0108750:CDS 40.0%
GTCATATATTGAGACAGTGC+TGG - chr1_3:20380675-20380694 Msa0108750:CDS 40.0%
GCATACATAGATTCGATTGC+AGG + chr1_3:20380755-20380774 None:intergenic 40.0%
GACTGTCTGATGAAGACTTA+TGG - chr1_3:20380856-20380875 Msa0108750:CDS 40.0%
GTTTCTTGACACCTGAATTC+TGG - chr1_3:20380878-20380897 Msa0108750:CDS 40.0%
CCAACATATCAGTGTACTCT+TGG + chr1_3:20380933-20380952 None:intergenic 40.0%
! CCAAGAGTACACTGATATGT+TGG - chr1_3:20380930-20380949 Msa0108750:CDS 40.0%
! AAGATCAAGGCCTTTGTTGT+GGG + chr1_3:20380961-20380980 None:intergenic 40.0%
AACCCTAGAAATGCAGAGAG+AGG + chr1_3:20378654-20378673 None:intergenic 45.0%
AGAGTGAGAGAAGGAGAATG+CGG + chr1_3:20378693-20378712 None:intergenic 45.0%
CACTCTCTCTCAACCAACAA+TGG - chr1_3:20378707-20378726 Msa0108750:three_prime_UTR 45.0%
GTTCTTTGATGGGAAGAGAG+TGG + chr1_3:20378938-20378957 None:intergenic 45.0%
!! CGACTAAGGTGTCGATGATT+TGG + chr1_3:20378962-20378981 None:intergenic 45.0%
ATCACTTCGTCTTTCAGCGT+GGG + chr1_3:20378987-20379006 None:intergenic 45.0%
!! GCTCTGGTTTGTTTCTGAAC+CGG + chr1_3:20379020-20379039 None:intergenic 45.0%
GTTCAGAAACAAACCAGAGC+CGG - chr1_3:20379020-20379039 Msa0108750:intron 45.0%
GTTGTCACCCACAACAACAA+AGG + chr1_3:20379066-20379085 None:intergenic 45.0%
GTTGTTGTGGGTGACAACAA+TGG - chr1_3:20379068-20379087 Msa0108750:intron 45.0%
GGTGACAACAATGGTCATGT+TGG - chr1_3:20379077-20379096 Msa0108750:intron 45.0%
GAAGTTGCTACTGCAATTCG+CGG - chr1_3:20379122-20379141 Msa0108750:intron 45.0%
AGTGTGATTCCTGTGAGAAG+AGG - chr1_3:20379167-20379186 Msa0108750:intron 45.0%
TTCCTGTGAGAAGAGGTTAC+TGG - chr1_3:20379174-20379193 Msa0108750:intron 45.0%
TCCTGTGAGAAGAGGTTACT+GGG - chr1_3:20379175-20379194 Msa0108750:intron 45.0%
GGTTACTGGGGGAATAAGAT+TGG - chr1_3:20379188-20379207 Msa0108750:intron 45.0%
GCAACAATACCAGAACCTCT+CGG + chr1_3:20379284-20379303 None:intergenic 45.0%
GTTCTGGTATTGTTGCTGCT+AGG - chr1_3:20379288-20379307 Msa0108750:intron 45.0%
TTCGCGCTTCTTAGCATACA+GGG + chr1_3:20379838-20379857 None:intergenic 45.0%
! GCTGGATGAGTAGTTTGTGT+TGG - chr1_3:20380693-20380712 Msa0108750:CDS 45.0%
CTTGACACCTGAATTCTGGA+AGG - chr1_3:20380882-20380901 Msa0108750:CDS 45.0%
CAAGATCAAGGCCTTTGTTG+TGG + chr1_3:20380962-20380981 None:intergenic 45.0%
CACAACAAAGGCCTTGATCT+TGG - chr1_3:20380960-20380979 Msa0108750:CDS 45.0%
AACAAAGGCCTTGATCTTGG+AGG - chr1_3:20380963-20380982 Msa0108750:CDS 45.0%
CTTGATCTTGGAGGAGGAAA+GGG - chr1_3:20380972-20380991 Msa0108750:CDS 45.0%
GGTTGAGAGAGAGTGAGAGA+AGG + chr1_3:20378702-20378721 None:intergenic 50.0%
TCCGAAACCTCTTCCGAATC+CGG + chr1_3:20378760-20378779 None:intergenic 50.0%
TGGGTCCCAGTAACAAAGCT+CGG - chr1_3:20378864-20378883 Msa0108750:CDS 50.0%
CTTTGATGGGAAGAGAGTGG+AGG + chr1_3:20378935-20378954 None:intergenic 50.0%
CATCACTTCGTCTTTCAGCG+TGG + chr1_3:20378988-20379007 None:intergenic 50.0%
TTTGAACCGGGTTCGTTGAC+CGG + chr1_3:20379042-20379061 None:intergenic 50.0%
CCTGTGAGAAGAGGTTACTG+GGG - chr1_3:20379176-20379195 Msa0108750:intron 50.0%
CTGTGAGAAGAGGTTACTGG+GGG - chr1_3:20379177-20379196 Msa0108750:intron 50.0%
ACCTTACACGGCACAGTATG+TGG + chr1_3:20379218-20379237 None:intergenic 50.0%
ACTGTGCCGTGTAAGGTTAC+TGG - chr1_3:20379221-20379240 Msa0108750:intron 50.0%
TGCTGCTAGGGTTCCTAAGA+AGG - chr1_3:20379301-20379320 Msa0108750:intron 50.0%
AGGGGATCTACCAAAACCCT+TGG - chr1_3:20379365-20379384 Msa0108750:intron 50.0%
GTTCGCGCTTCTTAGCATAC+AGG + chr1_3:20379839-20379858 None:intergenic 50.0%
! ATGCTAAGAAGCGCGAACAC+AGG - chr1_3:20379841-20379860 Msa0108750:intron 50.0%
CGAGTCTCCTTCCAGAATTC+AGG + chr1_3:20380892-20380911 None:intergenic 50.0%
GTTGGCAAAGCCCACAACAA+AGG - chr1_3:20380948-20380967 Msa0108750:CDS 50.0%
AAAGGCCTTGATCTTGGAGG+AGG - chr1_3:20380966-20380985 Msa0108750:CDS 50.0%
CCTTTCCTCCTCCAAGATCA+AGG + chr1_3:20380974-20380993 None:intergenic 50.0%
CCTTGATCTTGGAGGAGGAA+AGG - chr1_3:20380971-20380990 Msa0108750:CDS 50.0%
CAACCAACAATGGCAGACCG+AGG - chr1_3:20378717-20378736 Msa0108750:three_prime_UTR 55.0%
GCCGGATTCGGAAGAGGTTT+CGG - chr1_3:20378756-20378775 Msa0108750:three_prime_UTR 55.0%
GGAAGAGGTTTCGGAGATCG+TGG - chr1_3:20378765-20378784 Msa0108750:three_prime_UTR 55.0%
!! GACGGCCGAGCTTTGTTACT+GGG + chr1_3:20378872-20378891 None:intergenic 55.0%
!! AGACGGCCGAGCTTTGTTAC+TGG + chr1_3:20378873-20378892 None:intergenic 55.0%
TTTCAGCGTGGGACCGACTA+AGG + chr1_3:20378976-20378995 None:intergenic 55.0%
CCCCAGTAACCTCTTCTCAC+AGG + chr1_3:20379179-20379198 None:intergenic 55.0%
GCCACATACTGTGCCGTGTA+AGG - chr1_3:20379214-20379233 Msa0108750:intron 55.0%
! ATACCAGAACCTCTCGGTGC+AGG + chr1_3:20379278-20379297 None:intergenic 55.0%
GATCTTGGAGGAGGAAAGGG+CGG - chr1_3:20380975-20380994 Msa0108750:CDS 55.0%
GCTCCTCGGTCTGCCATTGT+TGG + chr1_3:20378723-20378742 None:intergenic 60.0%
ATGGCAGACCGAGGAGCTAC+CGG - chr1_3:20378726-20378745 Msa0108750:three_prime_UTR 60.0%
CTCTGTCTCCGGTAGCTCCT+CGG + chr1_3:20378737-20378756 None:intergenic 60.0%
ACCGGAGACAGAGCCGGATT+CGG - chr1_3:20378744-20378763 Msa0108750:three_prime_UTR 60.0%
TCCGAATCCGGCTCTGTCTC+CGG + chr1_3:20378748-20378767 None:intergenic 60.0%
GACAGAGCCGGATTCGGAAG+AGG - chr1_3:20378750-20378769 Msa0108750:three_prime_UTR 60.0%
GGTTTCGGAGATCGTGGTCG+AGG - chr1_3:20378771-20378790 Msa0108750:three_prime_UTR 60.0%
TTCGGAGATCGTGGTCGAGG+TGG - chr1_3:20378774-20378793 Msa0108750:three_prime_UTR 60.0%
CTCGGCCGTCTCGTCAAAGA+AGG - chr1_3:20378882-20378901 Msa0108750:CDS 60.0%
CGTATGGTTCCTGCACCGAG+AGG - chr1_3:20379266-20379285 Msa0108750:intron 60.0%
GTTCCTGCACCGAGAGGTTC+TGG - chr1_3:20379272-20379291 Msa0108750:intron 60.0%
GGAGCTACCGGAGACAGAGC+CGG - chr1_3:20378738-20378757 Msa0108750:three_prime_UTR 65.0%
GATCGTGGTCGAGGTGGCAG+AGG - chr1_3:20378780-20378799 Msa0108750:three_prime_UTR 65.0%
CCAGAGCCGGTCAACGAACC+CGG - chr1_3:20379033-20379052 Msa0108750:intron 65.0%
CGTGGTCGAGGTGGCAGAGG+CGG - chr1_3:20378783-20378802 Msa0108750:three_prime_UTR 70.0%
GGTGGCAGAGGCGGAGATCG+TGG - chr1_3:20378792-20378811 Msa0108750:three_prime_UTR 70.0%
AGAGGCGGAGATCGTGGCCG+TGG - chr1_3:20378798-20378817 Msa0108750:three_prime_UTR 70.0%
!! AGGCGCTGGCCGTCGTGAAG+AGG - chr1_3:20378833-20378852 Msa0108750:exon 70.0%
! CCGGGTTCGTTGACCGGCTC+TGG + chr1_3:20379036-20379055 None:intergenic 70.0%
GGCGGAGATCGTGGCCGTGG+AGG - chr1_3:20378801-20378820 Msa0108750:three_prime_UTR 75.0%
CGCCTCTGCGGCGACCTCCA+CGG + chr1_3:20378818-20378837 None:intergenic 75.0%
CACGACGGCCAGCGCCTCTG+CGG + chr1_3:20378830-20378849 None:intergenic 75.0%
! GGCCGTGGAGGTCGCCGCAG+AGG - chr1_3:20378813-20378832 Msa0108750:three_prime_UTR 80.0%
!! GGAGGTCGCCGCAGAGGCGC+TGG - chr1_3:20378819-20378838 Msa0108750:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 20378650 20381200 20378650 ID=Msa0108750;Name=Msa0108750
chr1_3 mRNA 20378650 20381200 20378650 ID=Msa0108750-mRNA-1;Parent=Msa0108750;Name=Msa0108750-mRNA-1;_AED=0.01;_eAED=0.01;_QI=76|1|1|1|1|1|2|195|277
chr1_3 exon 20378650 20379003 20378650 ID=Msa0108750-mRNA-1:exon:7397;Parent=Msa0108750-mRNA-1
chr1_3 exon 20380450 20381200 20380450 ID=Msa0108750-mRNA-1:exon:7396;Parent=Msa0108750-mRNA-1
chr1_3 five_prime_UTR 20381125 20381200 20381125 ID=Msa0108750-mRNA-1:five_prime_utr;Parent=Msa0108750-mRNA-1
chr1_3 CDS 20380450 20381124 20380450 ID=Msa0108750-mRNA-1:cds;Parent=Msa0108750-mRNA-1
chr1_3 CDS 20378845 20379003 20378845 ID=Msa0108750-mRNA-1:cds;Parent=Msa0108750-mRNA-1
chr1_3 three_prime_UTR 20378650 20378844 20378650 ID=Msa0108750-mRNA-1:three_prime_utr;Parent=Msa0108750-mRNA-1
Gene Sequence

>Msa0108750

ATGGCAGACCGAGGAGCTACCGGAGACAGAGCCGGATTCGGAAGAGGTTTCGGAGATCGTGGTCGAGGTGGCAGAGGCGGAGATCGTGGCCGTGGAGGTCGCCGCAGAGGCGCTGGCCGTCGTGAAGAGGAAGAAAAATGGGTCCCAGTAACAAAGCTCGGCCGTCTCGTCAAAGAAGGCAAAATCCGCAGCTTAGAACAAATCTACCTCCACTCTCTTCCCATCAAAGAACACCAAATCATCGACACCTTAGTCGGTCCCACGCTGAAAGACGAAGTGATGAAAATCATGCCGGTTCAGAAACAAACCAGAGCCGGTCAACGAACCCGGTTCAAAGCCTTTGTTGTTGTGGGTGACAACAATGGTCATGTTGGGTTAGGTGTGAAGTGTAGTAAGGAAGTTGCTACTGCAATTCGCGGTGCGATTATTCTTGCGAAATTGAGTGTGATTCCTGTGAGAAGAGGTTACTGGGGGAATAAGATTGGGAAGCCACATACTGTGCCGTGTAAGGTTACTGGAAAGTGTGGTTCTGTTACTGTTCGTATGGTTCCTGCACCGAGAGGTTCTGGTATTGTTGCTGCTAGGGTTCCTAAGAAGGTTTTGCAGTTTGCTGGAATTGATGATGTTTTTACTTCTTCAAGGGGATCTACCAAAACCCTTGGCAATTTCGTTAAGGCTACATTTGACTGTCTGATGAAGACTTATGGTTTCTTGACACCTGAATTCTGGAAGGAGACTCGCTTCAGTAAATCTCCATTCCAAGAGTACACTGATATGTTGGCAAAGCCCACAACAAAGGCCTTGATCTTGGAGGAGGAAAGGGCGGAAGCTTAA
Protein sequence

>Msa0108750

MADRGATGDRAGFGRGFGDRGRGGRGGDRGRGGRRRGAGRREEEEKWVPVTKLGRLVKEGKIRSLEQIYLHSLPIKEHQIIDTLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAFVVVGDNNGHVGLGVKCSKEVATAIRGAIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGKCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATFDCLMKTYGFLTPEFWKETRFSKSPFQEYTDMLAKPTTKALILEEERAEA*