Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0793700 | G7K3N7 | 93.023 | 172 | 10 | 2 | 1 | 171 | 1 | 171 | 1.04e-111 | 324 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0028610 | Msa0793700 | 0.802810 | 4.701982e-49 | -8.615850e-47 |
| Msa0662850 | Msa0793700 | 0.831000 | 2.139396e-55 | -8.615850e-47 |
| Msa0739570 | Msa0793700 | 0.817624 | 2.994501e-52 | -8.615850e-47 |
| Msa0782330 | Msa0793700 | 0.808842 | 2.538685e-50 | -8.615850e-47 |
| Msa0793700 | Msa0855490 | 0.808480 | 3.033854e-50 | -8.615850e-47 |
| Msa0793700 | Msa0860270 | 0.812407 | 4.306414e-51 | -8.615850e-47 |
| Msa0793700 | Msa1030280 | 0.800325 | 1.519620e-48 | -8.615850e-47 |
| Msa0793700 | Msa1049440 | 0.802764 | 4.805860e-49 | -8.615850e-47 |
| Msa0793700 | Msa1086060 | 0.812601 | 3.905703e-51 | -8.615850e-47 |
| Msa0793700 | Msa1422750 | 0.837994 | 3.741780e-57 | -8.615850e-47 |
| Msa0175900 | Msa0793700 | 0.810709 | 1.007368e-50 | -8.615850e-47 |
| Msa0249270 | Msa0793700 | 0.802479 | 5.502385e-49 | -8.615850e-47 |
| Msa0268730 | Msa0793700 | 0.809610 | 1.737503e-50 | -8.615850e-47 |
| Msa0306130 | Msa0793700 | 0.819150 | 1.350783e-52 | -8.615850e-47 |
| Msa0547050 | Msa0793700 | 0.802764 | 4.804319e-49 | -8.615850e-47 |
| Msa0582740 | Msa0793700 | 0.800399 | 1.468060e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0793700 | MtrunA17_Chr5g0394551 | 93.023 | 172 | 10 | 2 | 1 | 171 | 1 | 171 | 2.00e-115 | 324 |
| Msa0793700 | MtrunA17_Chr1g0205281 | 64.085 | 142 | 43 | 3 | 10 | 149 | 35 | 170 | 2.00e-57 | 178 |
| Msa0793700 | MtrunA17_Chr1g0185401 | 42.553 | 141 | 68 | 3 | 21 | 148 | 9 | 149 | 2.34e-27 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0793700 | AT3G22530.1 | 59.322 | 118 | 43 | 2 | 41 | 153 | 59 | 176 | 2.92e-40 | 135 |
| Msa0793700 | AT4G14830.1 | 63.793 | 116 | 38 | 3 | 41 | 152 | 28 | 143 | 1.15e-39 | 132 |
Find 39 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTGGAGGTTTCGTTTGC+CGG | 0.284682 | 5_3:-5589792 | Msa0793700:CDS |
| TCTTAGTTTGTTGTTGTTGT+TGG | 0.335493 | 5_3:+5590036 | None:intergenic |
| ATGATCTTCATCCTGATATC+TGG | 0.364083 | 5_3:-5589808 | Msa0793700:CDS |
| AAACGAAACCTCCAGATATC+AGG | 0.375554 | 5_3:+5589797 | None:intergenic |
| ACCTACCTGTTTCCGATTTG+TGG | 0.379340 | 5_3:-5589939 | Msa0793700:CDS |
| GAAACACTCTTGTCTGTGTC+TGG | 0.410833 | 5_3:-5590064 | Msa0793700:CDS |
| TTTGCCGGAATCTGTTGTTC+CGG | 0.426638 | 5_3:-5589777 | Msa0793700:CDS |
| CTCCGCCACAAATCGGAAAC+AGG | 0.434356 | 5_3:+5589934 | None:intergenic |
| AGCTCCGGAACAACAGATTC+CGG | 0.463331 | 5_3:+5589773 | None:intergenic |
| GAGACCATTCCTGAACCTAT+TGG | 0.472661 | 5_3:-5589689 | Msa0793700:CDS |
| GTCTCCCTCACGTGTTCAGC+AGG | 0.474728 | 5_3:-5590012 | Msa0793700:CDS |
| GTGTCACTAAGATTGTTGTA+AGG | 0.490134 | 5_3:-5589859 | Msa0793700:CDS |
| GAACCTATTGGTGGTGGTGG+TGG | 0.495438 | 5_3:-5589677 | Msa0793700:CDS |
| CCGACCGTAGCGGAAGTTGC+AGG | 0.500550 | 5_3:+5589987 | None:intergenic |
| CGTCTGTCTCCGCCACAAAT+CGG | 0.500659 | 5_3:+5589927 | None:intergenic |
| CCACCACCACCAATAGGTTC+AGG | 0.502881 | 5_3:+5589680 | None:intergenic |
| ACTCATCGTCACCGTGCCTA+AGG | 0.516775 | 5_3:-5589723 | Msa0793700:CDS |
| CCTGAACCTATTGGTGGTGG+TGG | 0.519897 | 5_3:-5589680 | Msa0793700:CDS |
| AACACAGCAGTAGCGAGCTC+CGG | 0.534860 | 5_3:+5589758 | None:intergenic |
| AGTGTTTCTGCTTGAGACAG+TGG | 0.538516 | 5_3:+5590079 | None:intergenic |
| GCTACTGCTGTGTTTGTCGA+TGG | 0.552059 | 5_3:-5589749 | Msa0793700:CDS |
| CACACGCTTCATATCCATCC+AGG | 0.559660 | 5_3:-5589881 | Msa0793700:CDS |
| TGTCACTAAGATTGTTGTAA+GGG | 0.575511 | 5_3:-5589858 | Msa0793700:CDS |
| ATCTTCATCCTGATATCTGG+AGG | 0.587374 | 5_3:-5589805 | Msa0793700:CDS |
| CAATCTTAGTGACACCTGGA+TGG | 0.590009 | 5_3:+5589867 | None:intergenic |
| GGATGCTGACGTGTCAGTTG+AGG | 0.599188 | 5_3:-5589966 | Msa0793700:CDS |
| ATTCCTGAACCTATTGGTGG+TGG | 0.599552 | 5_3:-5589683 | Msa0793700:CDS |
| CCTGCAACTTCCGCTACGGT+CGG | 0.606468 | 5_3:-5589987 | Msa0793700:CDS |
| AGACGCATCACTCGGACATG+TGG | 0.606866 | 5_3:-5589909 | Msa0793700:CDS |
| ACAACAATCTTAGTGACACC+TGG | 0.610797 | 5_3:+5589863 | None:intergenic |
| GTCTCATCGTCACAAGCCTT+AGG | 0.613900 | 5_3:+5589707 | None:intergenic |
| ACCATTCCTGAACCTATTGG+TGG | 0.619569 | 5_3:-5589686 | Msa0793700:CDS |
| AGGATCCTGCTGAACACGTG+AGG | 0.619719 | 5_3:+5590007 | None:intergenic |
| TACCTGTTTCCGATTTGTGG+CGG | 0.624557 | 5_3:-5589936 | Msa0793700:CDS |
| GGATCCTGCTGAACACGTGA+GGG | 0.626553 | 5_3:+5590008 | None:intergenic |
| GGATCCTGCAACTTCCGCTA+CGG | 0.628844 | 5_3:-5589991 | Msa0793700:CDS |
| ACCACCAATAGGTTCAGGAA+TGG | 0.647164 | 5_3:+5589685 | None:intergenic |
| ATCGTCACAAGCCTTAGGCA+CGG | 0.656268 | 5_3:+5589712 | None:intergenic |
| GCCACAAATCGGAAACAGGT+AGG | 0.667827 | 5_3:+5589938 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCAATGAGTTGCAAAAAA+TGG | - | chr5_3:5589665-5589684 | Msa0793700:CDS | 30.0% | |
| !! | TCTTAGTTTGTTGTTGTTGT+TGG | + | chr5_3:5589764-5589783 | None:intergenic | 30.0% |
| TGTCACTAAGATTGTTGTAA+GGG | - | chr5_3:5589939-5589958 | Msa0793700:CDS | 30.0% | |
| ! | GTGTCACTAAGATTGTTGTA+AGG | - | chr5_3:5589938-5589957 | Msa0793700:CDS | 35.0% |
| !! | TCACTCACTTCACTTTTCTT+TGG | - | chr5_3:5589966-5589985 | Msa0793700:CDS | 35.0% |
| ATGATCTTCATCCTGATATC+TGG | - | chr5_3:5589989-5590008 | Msa0793700:CDS | 35.0% | |
| ACAACAATCTTAGTGACACC+TGG | + | chr5_3:5589937-5589956 | None:intergenic | 40.0% | |
| ATCTTCATCCTGATATCTGG+AGG | - | chr5_3:5589992-5590011 | Msa0793700:CDS | 40.0% | |
| AAACGAAACCTCCAGATATC+AGG | + | chr5_3:5590003-5590022 | None:intergenic | 40.0% | |
| ! | AGTGTTTCTGCTTGAGACAG+TGG | + | chr5_3:5589721-5589740 | None:intergenic | 45.0% |
| GAAACACTCTTGTCTGTGTC+TGG | - | chr5_3:5589733-5589752 | Msa0793700:CDS | 45.0% | |
| ! | ACCTACCTGTTTCCGATTTG+TGG | - | chr5_3:5589858-5589877 | Msa0793700:CDS | 45.0% |
| ! | TACCTGTTTCCGATTTGTGG+CGG | - | chr5_3:5589861-5589880 | Msa0793700:CDS | 45.0% |
| CAATCTTAGTGACACCTGGA+TGG | + | chr5_3:5589933-5589952 | None:intergenic | 45.0% | |
| TATCTGGAGGTTTCGTTTGC+CGG | - | chr5_3:5590005-5590024 | Msa0793700:CDS | 45.0% | |
| TTTGCCGGAATCTGTTGTTC+CGG | - | chr5_3:5590020-5590039 | Msa0793700:CDS | 45.0% | |
| GAGACCATTCCTGAACCTAT+TGG | - | chr5_3:5590108-5590127 | Msa0793700:CDS | 45.0% | |
| ACCATTCCTGAACCTATTGG+TGG | - | chr5_3:5590111-5590130 | Msa0793700:CDS | 45.0% | |
| ACCACCAATAGGTTCAGGAA+TGG | + | chr5_3:5590115-5590134 | None:intergenic | 45.0% | |
| ATTCCTGAACCTATTGGTGG+TGG | - | chr5_3:5590114-5590133 | Msa0793700:CDS | 45.0% | |
| GCCACAAATCGGAAACAGGT+AGG | + | chr5_3:5589862-5589881 | None:intergenic | 50.0% | |
| CACACGCTTCATATCCATCC+AGG | - | chr5_3:5589916-5589935 | Msa0793700:CDS | 50.0% | |
| AGCTCCGGAACAACAGATTC+CGG | + | chr5_3:5590027-5590046 | None:intergenic | 50.0% | |
| GCTACTGCTGTGTTTGTCGA+TGG | - | chr5_3:5590048-5590067 | Msa0793700:CDS | 50.0% | |
| ATCGTCACAAGCCTTAGGCA+CGG | + | chr5_3:5590088-5590107 | None:intergenic | 50.0% | |
| GTCTCATCGTCACAAGCCTT+AGG | + | chr5_3:5590093-5590112 | None:intergenic | 50.0% | |
| GGATCCTGCTGAACACGTGA+GGG | + | chr5_3:5589792-5589811 | None:intergenic | 55.0% | |
| AGGATCCTGCTGAACACGTG+AGG | + | chr5_3:5589793-5589812 | None:intergenic | 55.0% | |
| GGATCCTGCAACTTCCGCTA+CGG | - | chr5_3:5589806-5589825 | Msa0793700:CDS | 55.0% | |
| GGATGCTGACGTGTCAGTTG+AGG | - | chr5_3:5589831-5589850 | Msa0793700:CDS | 55.0% | |
| CTCCGCCACAAATCGGAAAC+AGG | + | chr5_3:5589866-5589885 | None:intergenic | 55.0% | |
| CGTCTGTCTCCGCCACAAAT+CGG | + | chr5_3:5589873-5589892 | None:intergenic | 55.0% | |
| AGACGCATCACTCGGACATG+TGG | - | chr5_3:5589888-5589907 | Msa0793700:CDS | 55.0% | |
| AACACAGCAGTAGCGAGCTC+CGG | + | chr5_3:5590042-5590061 | None:intergenic | 55.0% | |
| ACTCATCGTCACCGTGCCTA+AGG | - | chr5_3:5590074-5590093 | Msa0793700:CDS | 55.0% | |
| CCACCACCACCAATAGGTTC+AGG | + | chr5_3:5590120-5590139 | None:intergenic | 55.0% | |
| !! | CCTGAACCTATTGGTGGTGG+TGG | - | chr5_3:5590117-5590136 | Msa0793700:CDS | 55.0% |
| !! | GAACCTATTGGTGGTGGTGG+TGG | - | chr5_3:5590120-5590139 | Msa0793700:CDS | 55.0% |
| GTCTCCCTCACGTGTTCAGC+AGG | - | chr5_3:5589785-5589804 | Msa0793700:CDS | 60.0% | |
| CCTGCAACTTCCGCTACGGT+CGG | - | chr5_3:5589810-5589829 | Msa0793700:CDS | 60.0% | |
| ACGTCAGCATCCGACCGTAG+CGG | + | chr5_3:5589823-5589842 | None:intergenic | 60.0% | |
| GCGGAGACAGACGCATCACT+CGG | - | chr5_3:5589880-5589899 | Msa0793700:CDS | 60.0% | |
| GCACCACCACCACCACCAAT+AGG | + | chr5_3:5590126-5590145 | None:intergenic | 60.0% | |
| ! | CCGACCGTAGCGGAAGTTGC+AGG | + | chr5_3:5589813-5589832 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 5589652 | 5590167 | 5589652 | ID=Msa0793700;Name=Msa0793700 |
| chr5_3 | mRNA | 5589652 | 5590167 | 5589652 | ID=Msa0793700-mRNA-1;Parent=Msa0793700;Name=Msa0793700-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|171 |
| chr5_3 | exon | 5589652 | 5590167 | 5589652 | ID=Msa0793700-mRNA-1:exon:2230;Parent=Msa0793700-mRNA-1 |
| chr5_3 | CDS | 5589652 | 5590167 | 5589652 | ID=Msa0793700-mRNA-1:cds;Parent=Msa0793700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
