AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268730 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268730 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0268730 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268730 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0268730 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 2_2:+70380713 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 2_2:-70380816 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 2_2:+70381364 Msa0268730:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 2_2:+70381352 Msa0268730:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 2_2:-70381370 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 2_2:-70381357 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr2_2:70381005-70381024 Msa0268730:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr2_2:70381011-70381030 None:intergenic 10.0%
!! AATTTCAAAAAAAAAAACTT+AGG - chr2_2:70381162-70381181 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr2_2:70381003-70381022 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr2_2:70381256-70381275 Msa0268730:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr2_2:70380760-70380779 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr2_2:70380761-70380780 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr2_2:70380761-70380780 Msa0268730:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr2_2:70381049-70381068 Msa0268730:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr2_2:70381100-70381119 Msa0268730:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr2_2:70381190-70381209 Msa0268730:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr2_2:70380853-70380872 Msa0268730:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr2_2:70381331-70381350 Msa0268730:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr2_2:70380802-70380821 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr2_2:70380819-70380838 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr2_2:70380837-70380856 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr2_2:70380846-70380865 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr2_2:70380847-70380866 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr2_2:70381081-70381100 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr2_2:70381084-70381103 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr2_2:70381082-70381101 Msa0268730:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr2_2:70381090-70381109 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr2_2:70381233-70381252 Msa0268730:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr2_2:70380795-70380814 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr2_2:70381352-70381371 Msa0268730:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr2_2:70381360-70381379 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr2_2:70381078-70381097 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr2_2:70381364-70381383 Msa0268730:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr2_2:70380924-70380943 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr2_2:70381373-70381392 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70380714 70381399 70380714 ID=Msa0268730;Name=Msa0268730
chr2_2 mRNA 70380714 70381399 70380714 ID=Msa0268730-mRNA-1;Parent=Msa0268730;Name=Msa0268730-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr2_2 exon 70380714 70380888 70380714 ID=Msa0268730-mRNA-1:exon:22606;Parent=Msa0268730-mRNA-1
chr2_2 exon 70381281 70381399 70381281 ID=Msa0268730-mRNA-1:exon:22607;Parent=Msa0268730-mRNA-1
chr2_2 CDS 70380714 70380888 70380714 ID=Msa0268730-mRNA-1:cds;Parent=Msa0268730-mRNA-1
chr2_2 CDS 70381281 70381399 70381281 ID=Msa0268730-mRNA-1:cds;Parent=Msa0268730-mRNA-1
Gene Sequence

>Msa0268730

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0268730

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*