AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0582740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582740 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0582740 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0582740 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582740 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0582740 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 4_2:+1186551 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 4_2:-1186654 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 4_2:+1187201 Msa0582740:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 4_2:+1187189 Msa0582740:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 4_2:-1187207 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 4_2:-1187194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr4_2:1186841-1186860 Msa0582740:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr4_2:1186847-1186866 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr4_2:1186998-1187017 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr4_2:1186839-1186858 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr4_2:1187093-1187112 Msa0582740:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr4_2:1186598-1186617 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr4_2:1186599-1186618 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr4_2:1186599-1186618 Msa0582740:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr4_2:1186885-1186904 Msa0582740:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr4_2:1186936-1186955 Msa0582740:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr4_2:1187027-1187046 Msa0582740:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr4_2:1186691-1186710 Msa0582740:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr4_2:1187168-1187187 Msa0582740:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr4_2:1186640-1186659 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr4_2:1186657-1186676 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr4_2:1186675-1186694 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr4_2:1186684-1186703 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr4_2:1186685-1186704 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr4_2:1186917-1186936 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr4_2:1186920-1186939 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr4_2:1186918-1186937 Msa0582740:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr4_2:1186926-1186945 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr4_2:1187070-1187089 Msa0582740:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr4_2:1186633-1186652 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr4_2:1187189-1187208 Msa0582740:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr4_2:1187197-1187216 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr4_2:1186914-1186933 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr4_2:1187201-1187220 Msa0582740:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr4_2:1186762-1186781 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr4_2:1187210-1187229 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 1186552 1187236 1186552 ID=Msa0582740;Name=Msa0582740
chr4_2 mRNA 1186552 1187236 1186552 ID=Msa0582740-mRNA-1;Parent=Msa0582740;Name=Msa0582740-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr4_2 exon 1186552 1186726 1186552 ID=Msa0582740-mRNA-1:exon:298;Parent=Msa0582740-mRNA-1
chr4_2 exon 1187118 1187236 1187118 ID=Msa0582740-mRNA-1:exon:299;Parent=Msa0582740-mRNA-1
chr4_2 CDS 1186552 1186726 1186552 ID=Msa0582740-mRNA-1:cds;Parent=Msa0582740-mRNA-1
chr4_2 CDS 1187118 1187236 1187118 ID=Msa0582740-mRNA-1:cds;Parent=Msa0582740-mRNA-1
Gene Sequence

>Msa0582740

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0582740

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*