AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086060 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086060 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa1086060 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086060 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa1086060 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 7_2:+87110879 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 7_2:-87110982 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 7_2:+87111529 Msa1086060:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 7_2:+87111517 Msa1086060:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 7_2:-87111535 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 7_2:-87111522 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr7_2:87111169-87111188 Msa1086060:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr7_2:87111175-87111194 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr7_2:87111326-87111345 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr7_2:87111167-87111186 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr7_2:87111421-87111440 Msa1086060:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr7_2:87110926-87110945 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr7_2:87110927-87110946 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr7_2:87110927-87110946 Msa1086060:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr7_2:87111213-87111232 Msa1086060:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr7_2:87111264-87111283 Msa1086060:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr7_2:87111355-87111374 Msa1086060:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr7_2:87111019-87111038 Msa1086060:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr7_2:87111496-87111515 Msa1086060:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr7_2:87110968-87110987 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr7_2:87110985-87111004 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr7_2:87111003-87111022 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr7_2:87111012-87111031 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr7_2:87111013-87111032 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr7_2:87111245-87111264 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr7_2:87111248-87111267 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr7_2:87111246-87111265 Msa1086060:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr7_2:87111254-87111273 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr7_2:87111398-87111417 Msa1086060:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr7_2:87110961-87110980 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr7_2:87111517-87111536 Msa1086060:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr7_2:87111525-87111544 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr7_2:87111242-87111261 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr7_2:87111529-87111548 Msa1086060:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr7_2:87111090-87111109 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr7_2:87111538-87111557 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87110880 87111564 87110880 ID=Msa1086060;Name=Msa1086060
chr7_2 mRNA 87110880 87111564 87110880 ID=Msa1086060-mRNA-1;Parent=Msa1086060;Name=Msa1086060-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr7_2 exon 87110880 87111054 87110880 ID=Msa1086060-mRNA-1:exon:23706;Parent=Msa1086060-mRNA-1
chr7_2 exon 87111446 87111564 87111446 ID=Msa1086060-mRNA-1:exon:23707;Parent=Msa1086060-mRNA-1
chr7_2 CDS 87110880 87111054 87110880 ID=Msa1086060-mRNA-1:cds;Parent=Msa1086060-mRNA-1
chr7_2 CDS 87111446 87111564 87111446 ID=Msa1086060-mRNA-1:cds;Parent=Msa1086060-mRNA-1
Gene Sequence

>Msa1086060

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa1086060

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*