AlfalfaGEDB | Msa0794840

M. sativa cultivar zhongmu-4 / Msa0794840

KEGG: K10571
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360
PFAM: PF00349.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0794840	sp\|Q9ZNU6\|DET1_SOLLC	74.610	449	85	4	226	671	25	447	0.0	690

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0794840	A0A072UDA3	95.556	450	4	2	226	672	28	464	0.0	886

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0119320	Msa0794840	0.839272	1.749939e-57	-8.615850e-47
Msa0138210	Msa0794840	0.805986	1.024316e-49	-8.615850e-47
Msa0194720	Msa0794840	0.807637	4.586542e-50	-8.615850e-47
Msa0227230	Msa0794840	0.805361	1.385418e-49	-8.615850e-47
Msa0229940	Msa0794840	0.827331	1.659660e-54	-8.615850e-47
Msa0277420	Msa0794840	0.809112	2.222955e-50	-8.615850e-47
Msa0301810	Msa0794840	0.800012	1.759479e-48	-8.615850e-47
Msa0388650	Msa0794840	0.808138	3.588891e-50	-8.615850e-47
Msa0417730	Msa0794840	0.801238	9.896430e-49	-8.615850e-47
Msa0442310	Msa0794840	0.812493	4.123151e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0794840	MtrunA17_Chr5g0396271	95.111	450	6	2	226	672	28	464	0.0	884
Msa0794840	MtrunA17_Chr5g0396421	98.238	227	4	0	1	227	1	227	1.94e-157	463
Msa0794840	MtrunA17_Chr8g0389821	45.847	301	140	6	27	309	27	322	4.73e-74	247
Msa0794840	MtrunA17_Chr8g0340741	56.769	229	96	2	1	227	1	228	1.01e-70	238
Msa0794840	MtrunA17_Chr6g0487071	53.807	197	89	1	33	227	31	227	1.94e-65	224
Msa0794840	MtrunA17_Chr1g0156571	50.495	202	98	1	28	227	30	231	1.20e-54	194

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0794840	AT4G10180.1	68.113	461	122	6	223	672	22	468	0.0	619
Msa0794840	AT1G50460.2	67.157	204	66	1	25	227	25	228	1.22e-87	279
Msa0794840	AT1G50460.1	67.157	204	66	1	25	227	25	228	2.70e-86	280
Msa0794840	AT3G20040.1	61.572	229	86	1	1	227	1	229	9.88e-84	273
Msa0794840	AT4G29130.1	59.389	229	91	1	1	227	1	229	1.89e-76	253
Msa0794840	AT2G19860.1	59.825	229	90	1	1	227	1	229	3.69e-76	253
Msa0794840	AT1G47840.2	51.832	191	89	1	40	227	43	233	9.62e-55	192
Msa0794840	AT1G47840.1	51.832	191	89	1	40	227	43	233	1.15e-53	192
Msa0794840	AT1G50460.3	62.500	136	50	1	93	227	17	152	1.31e-46	171
Msa0794840	AT2G19860.2	54.622	119	52	1	111	227	2	120	4.80e-29	120

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Find 160 sgRNAs with CRISPR-Local

Find 526 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTGTTCCGAAGTCTT+TGG	0.299663	5_3:-6760615	None:intergenic
AAAAGAGACTGGTCAAAGTA+AGG	0.490331	5_3:-6762029	None:intergenic
AAACAAATGTCTGTTTCCTC+GGG	0.521874	5_3:+6756318	Msa0794840:CDS
AAACAGACATTTGTTTCATA+GGG	0.475582	5_3:-6756311	None:intergenic
AAAGAAAGAAGGGGCGGCTC+CGG	0.461070	5_3:-6754203	None:intergenic
AAATCACGTGCAGCCTAATA+TGG	0.236617	5_3:+6759400	Msa0794840:CDS
AACTCCGGTGAAACGGGAAA+CGG	0.486811	5_3:-6754287	None:intergenic
AAGAGTGGTAGTGGGAGTGA+CGG	0.625333	5_3:+6754477	Msa0794840:CDS
AAGATGGGAAGAGTGGTAGT+GGG	0.588287	5_3:+6754469	Msa0794840:exon
AAGGCAAGTGGTTGATGCTA+TGG	0.522670	5_3:+6754636	Msa0794840:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGAGAGAAAAAGAAAGAA+GGG	-	chr5_3:6754216-6754235	None:intergenic	30.0%
TGATTCGATTTAACAAACTC+CGG	-	chr5_3:6754305-6754324	None:intergenic	30.0%
GTTAGGAAGATTATCAACAT+AGG	-	chr5_3:6754722-6754741	None:intergenic	30.0%
AAAAAAAGTGATTACCCGTT+AGG	-	chr5_3:6754739-6754758	None:intergenic	30.0%
TGGAAAATGTACTTTGGATT+TGG	+	chr5_3:6755109-6755128	Msa0794840:intron	30.0%
TTGATAGATTGTGTTTGGAT+TGG	+	chr5_3:6755225-6755244	Msa0794840:intron	30.0%
TGATTGATAGATTGTGTGTT+TGG	+	chr5_3:6755570-6755589	Msa0794840:intron	30.0%
AGTCTATGACAGCTATTTAT+TGG	+	chr5_3:6755635-6755654	Msa0794840:intron	30.0%
GTCTATGACAGCTATTTATT+GGG	+	chr5_3:6755636-6755655	Msa0794840:intron	30.0%
TCCTTATCTTTAGCAAGATT+TGG	+	chr5_3:6755829-6755848	Msa0794840:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	6754203	6762085	6754203	ID=Msa0794840;Name=Msa0794840
chr5_3	mRNA	6754203	6762085	6754203	ID=Msa0794840-mRNA-1;Parent=Msa0794840;Name=Msa0794840-mRNA-1;_AED=0.25;_eAED=0.25;_QI=269\|0.81\|0.66\|1\|1\|1\|12\|0\|673
chr5_3	exon	6754203	6754743	6754203	ID=Msa0794840-mRNA-1:exon:2731;Parent=Msa0794840-mRNA-1
chr5_3	exon	6755953	6756103	6755953	ID=Msa0794840-mRNA-1:exon:2732;Parent=Msa0794840-mRNA-1
chr5_3	exon	6756207	6756383	6756207	ID=Msa0794840-mRNA-1:exon:2733;Parent=Msa0794840-mRNA-1
chr5_3	exon	6756544	6756618	6756544	ID=Msa0794840-mRNA-1:exon:2734;Parent=Msa0794840-mRNA-1
chr5_3	exon	6758445	6758872	6758445	ID=Msa0794840-mRNA-1:exon:2735;Parent=Msa0794840-mRNA-1
chr5_3	exon	6758973	6759201	6758973	ID=Msa0794840-mRNA-1:exon:2736;Parent=Msa0794840-mRNA-1
chr5_3	exon	6759344	6759508	6759344	ID=Msa0794840-mRNA-1:exon:2737;Parent=Msa0794840-mRNA-1
chr5_3	exon	6760610	6760678	6760610	ID=Msa0794840-mRNA-1:exon:2738;Parent=Msa0794840-mRNA-1
chr5_3	exon	6760819	6760875	6760819	ID=Msa0794840-mRNA-1:exon:2739;Parent=Msa0794840-mRNA-1
chr5_3	exon	6761455	6761574	6761455	ID=Msa0794840-mRNA-1:exon:2740;Parent=Msa0794840-mRNA-1
chr5_3	exon	6761674	6761838	6761674	ID=Msa0794840-mRNA-1:exon:2741;Parent=Msa0794840-mRNA-1
chr5_3	exon	6761972	6762085	6761972	ID=Msa0794840-mRNA-1:exon:2742;Parent=Msa0794840-mRNA-1
chr5_3	five_prime_UTR	6754203	6754471	6754203	ID=Msa0794840-mRNA-1:five_prime_utr;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6754472	6754743	6754472	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6755953	6756103	6755953	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6756207	6756383	6756207	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6756544	6756618	6756544	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6758445	6758872	6758445	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6758973	6759201	6758973	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6759344	6759508	6759344	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6760610	6760678	6760610	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6760819	6760875	6760819	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6761455	6761574	6761455	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6761674	6761838	6761674	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1
chr5_3	CDS	6761972	6762085	6761972	ID=Msa0794840-mRNA-1:cds;Parent=Msa0794840-mRNA-1

Gene Sequence

>Msa0794840

ATGGGAAGAGTGGTAGTGGGAGTGACGGCGACGGTGGCGGTGGCGGCGTGTGCGGTGGCGGCGGTTATTGTCGCTAGAAGAGTGAAGAGTAGAAGAAAGTGGAAGAAAGTAGCGAATGTGTTGAAAGAGTTTGAAGAAGGTTGTGATACGAGTGTTGAAAGGTTAAGGCAAGTGGTTGATGCTATGGCGGTGGAGATGCACGCCGGTTTAGCTTCTGAAGGTGGTTCTAAGCTCAAAATGCTTCTTACCTATGTTGATAATCTTCCTAACGGTACTGAGAAAGGACCATATTATGCGCTGCATCTTGGGGGAACAAATTTTAGGGTAACGCGGGTTCATTTAAGTGGTCAACCATCTCCCGTCCTTGAGCATGAAGTAGAGCGACAACCTATTCCCCCACATCTAATGACTGGCACAAGCAAGGATCTCTTTGATTTTATCGCATCATCGTTAAAAGAATTCATTGCTAAAGAAGATGGTTCCAATACTTCACAGGACAGAAGGGAACTTGGCTTTACATTCTCCTTCCCTATGAAACAAATGTCTGTTTCCTCGGGCATTTTAATCAAGTGGACAAAAGGGTTTTCCATTGTAGATATGGTTGGAAGAGATGTTGCAGCATGTTTGCAGGAAGCATTTGCACGAAATGGCCTAGATGTGCATGTAGCTGCCTTGGTTCATCATGCTCGGCGATTCTATGAAAATTTGGTGCCAAGTTACACAATATATGAGGTTGAGTGCCCAGACCATTCATTTCGTAAATTCACAGAGGATGGTCAATACCTTATAAGTTTCAGCAGAAACCATCAAGAGTTGATTGTTTATAGGCCTAGATGGCTTTCTTTTTCATGCAATGATGAAGATTGTGATCAACATGATTTGCCGTCAAAAGCAAGAAGATTCGACAGTTTTTTCACTCAATTATATTGTGTACCACTTGCTTCTTGCAATGAACTCATATGTAAAGATTTCTTTCTTTACATGGAGGGTAATCAATTTGGACTGTTTGCCACTTCTACGGCCCAAATTCATGATGCACCTGCTGTTGGAGGAGCTGTCCAGGGTGTTCCTTCAATAGAAAAGATAACTTTTCACCTTTTGAGAATGGAAGATGGAGAGATCCTGGATAAGAAAGTCTTCAGTAATGACTTTGTTAACTTGACCCATAATATGGGTGTCTTCTTGTATGATGATCTATTGGCAATTGTATCACTTCGGTATCAAACTATACACATTCTTCAAATTAGGGATTCTGGTAACCTTGTTGACGTTCGTGCTATTGGGGAATTCTGTCGTGAAGACGACGAACTTTTTCTCAATTCAAATGCTCAGGGCATGTCATTGTCTGACAAAAATAAACAGCAGCAGGTACCAGAGAACAATGTAGAAAATCACGTGCAGCCTAATATGGGGAATTCTTTTTTAAGTGGTATAAAGCAGCGATTGCTTTCATTCATATTCCAGGGAATATGGAATGAAGAAACAGATGATACCTTGAGGATCCAAAGACTTCGGAACAAGTTTTACTTCCACTTTCAAGATTACGTTGATCTGATTATTTGGAAGGTGCAATTCTTGGACCGACACCACTTGCTAATTAAGTTTGGCAGCGTAGATGGAGGGTATGACAGACGTATTGATATTCATGTTTTGAAGCCTCAGGTATCAAGAAATGCTGATCACCATCCAGCTTTTGTTGCTGTATATAACATGGCTACAACTGAAATTGTATCTTTTTATCAGAATTCAGCAGACGAGCTTTATATGTTGTTTGAGCAGTTTGGTGACCACTTTCATGTAACGTCAAGGAATTCAATGTATATGAATTTCGTATCATCTCATTCAAATAATATTCATGCACTAGAACAGCTACGGAGCATAAAGGATAAGGCAAGCAATTCTTCACAGTTTGTGAAGAAGATGCTTGCCTCTTTGCCTTTTAGCTGTCAGTCACAGAGTCCTTCTCCTTACTTTGACCAGTCTCTTTTCCGGTTTGATGATAAGGTTTACTCCACATACTAG

Protein sequence

>Msa0794840

MGRVVVGVTATVAVAACAVAAVIVARRVKSRRKWKKVANVLKEFEEGCDTSVERLRQVVDAMAVEMHAGLASEGGSKLKMLLTYVDNLPNGTEKGPYYALHLGGTNFRVTRVHLSGQPSPVLEHEVERQPIPPHLMTGTSKDLFDFIASSLKEFIAKEDGSNTSQDRRELGFTFSFPMKQMSVSSGILIKWTKGFSIVDMVGRDVAACLQEAFARNGLDVHVAALVHHARRFYENLVPSYTIYEVECPDHSFRKFTEDGQYLISFSRNHQELIVYRPRWLSFSCNDEDCDQHDLPSKARRFDSFFTQLYCVPLASCNELICKDFFLYMEGNQFGLFATSTAQIHDAPAVGGAVQGVPSIEKITFHLLRMEDGEILDKKVFSNDFVNLTHNMGVFLYDDLLAIVSLRYQTIHILQIRDSGNLVDVRAIGEFCREDDELFLNSNAQGMSLSDKNKQQQVPENNVENHVQPNMGNSFLSGIKQRLLSFIFQGIWNEETDDTLRIQRLRNKFYFHFQDYVDLIIWKVQFLDRHHLLIKFGSVDGGYDRRIDIHVLKPQVSRNADHHPAFVAVYNMATTEIVSFYQNSADELYMLFEQFGDHFHVTSRNSMYMNFVSSHSNNIHALEQLRSIKDKASNSSQFVKKMLASLPFSCQSQSPSPYFDQSLFRFDDKVYSTY*