AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0227230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227230 MtrunA17_Chr2g0325391 93.638 613 37 1 1 613 1 611 0.0 1147
Msa0227230 MtrunA17_Chr2g0325411 90.777 618 43 3 1 606 1 616 0.0 1114
Msa0227230 MtrunA17_Chr4g0040981 66.017 462 147 7 136 595 497 950 0.0 585
Msa0227230 MtrunA17_Chr4g0049471 44.954 327 127 9 254 549 83 387 1.59e-73 244
Msa0227230 MtrunA17_Chr4g0049491 45.367 313 128 8 265 549 128 425 8.96e-70 236
Msa0227230 MtrunA17_Chr3g0116311 39.313 262 118 6 297 549 207 436 1.89e-45 172
Msa0227230 MtrunA17_Chr7g0230041 37.931 261 136 3 294 553 180 415 5.84e-45 171
Msa0227230 MtrunA17_Chr2g0285151 56.190 105 46 0 300 404 83 187 1.22e-34 138
Msa0227230 MtrunA17_Chr8g0369041 29.268 246 155 4 293 535 90 319 6.54e-29 118
Msa0227230 MtrunA17_Chr6g0462121 31.120 241 137 5 303 535 88 307 8.30e-28 115
Msa0227230 MtrunA17_Chr7g0230031 59.494 79 32 0 294 372 195 273 3.27e-25 110
Msa0227230 MtrunA17_Chr2g0296681 30.131 229 132 6 311 535 94 298 5.43e-18 85.9
Msa0227230 MtrunA17_Chr2g0277091 38.298 94 57 1 303 395 202 295 2.93e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227230 AT1G06230.2 59.483 464 167 8 144 594 272 727 2.84e-163 486
Msa0227230 AT1G06230.4 59.483 464 167 8 144 594 272 727 2.84e-163 486
Msa0227230 AT1G06230.1 59.483 464 167 8 144 594 272 727 2.84e-163 486
Msa0227230 AT1G06230.3 59.483 464 167 8 144 594 272 727 2.84e-163 486
Msa0227230 AT1G73150.2 50.316 316 129 6 263 559 86 392 1.46e-84 272
Msa0227230 AT1G73150.1 50.316 316 129 6 263 559 86 392 2.55e-84 272
Msa0227230 AT1G17790.1 44.762 315 137 7 287 574 122 426 5.56e-72 241
Msa0227230 AT3G27260.4 40.323 248 116 6 296 537 176 397 5.23e-43 165
Msa0227230 AT3G27260.3 40.323 248 116 6 296 537 176 397 1.15e-42 165
Msa0227230 AT3G27260.1 40.486 247 115 6 297 537 177 397 1.21e-42 165
Msa0227230 AT3G27260.2 40.323 248 116 6 296 537 127 348 1.36e-42 164
Msa0227230 AT5G14270.2 37.066 259 135 5 293 550 134 365 7.24e-42 162
Msa0227230 AT5G14270.3 37.066 259 135 5 293 550 134 365 7.53e-42 161
Msa0227230 AT5G14270.1 37.066 259 135 5 293 550 134 365 7.53e-42 161
Msa0227230 AT3G01770.2 38.559 236 115 5 297 531 130 336 1.25e-39 152
Msa0227230 AT3G01770.1 38.559 236 115 5 297 531 130 336 4.88e-39 152
Msa0227230 AT5G63320.3 31.818 330 183 8 221 544 95 388 3.96e-36 142
Msa0227230 AT5G63320.2 31.818 330 183 8 221 544 95 388 3.96e-36 142
Msa0227230 AT5G63320.1 35.409 257 135 6 293 544 158 388 1.36e-35 144
Msa0227230 AT5G65630.1 62.000 100 38 0 292 391 165 264 1.13e-33 136
Msa0227230 AT5G65630.1 54.717 106 47 1 444 549 374 478 1.24e-27 118
Msa0227230 AT2G34900.2 30.522 249 155 3 297 544 1 232 3.73e-32 126
Msa0227230 AT5G10550.1 59.000 100 41 0 292 391 170 269 4.57e-32 131
Msa0227230 AT5G10550.1 57.292 96 41 0 454 549 378 473 1.56e-26 115
Msa0227230 AT2G34900.1 30.522 249 155 3 297 544 111 342 6.88e-32 128
Msa0227230 AT5G46550.1 47.107 121 57 2 300 414 73 192 2.08e-28 119
Msa0227230 AT3G52280.1 27.459 244 158 4 297 537 95 322 2.95e-24 105
Msa0227230 AT3G52280.2 27.686 242 156 4 297 535 112 337 3.58e-24 105

Find 202 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAGGGAAAATAAGAATT+AGG 0.148252 2_1:-78007988 Msa0227230:five_prime_UTR
TCATCGTCTGGGTCTGATTC+AGG 0.166031 2_1:-78005777 Msa0227230:CDS
TACATCTACGTATTGGAATT+TGG 0.179690 2_1:-78005518 Msa0227230:three_prime_UTR
ACATGAACATCTTGCCCTTT+AGG 0.234304 2_1:+78006835 None:intergenic
ATGAGGTATGGCTTGGATTA+TGG 0.238032 2_1:-78006742 Msa0227230:CDS
AAACCCTAGATTTGAATTCT+AGG 0.247154 2_1:-78008038 Msa0227230:intron
GGAGAAATGGGACATGGATT+TGG 0.255723 2_1:-78007105 Msa0227230:CDS
CTAACACCATTACACTTAAC+TGG 0.281372 2_1:+78007286 None:intergenic
CGGTGAGGTCGTTGGTGATT+AGG 0.282820 2_1:-78007461 Msa0227230:CDS
CTCCGCCAGTTGTGAATTCT+AGG 0.296493 2_1:-78007707 Msa0227230:CDS
CGAGTCAAGCTCAAGGGATT+TGG 0.299517 2_1:+78006613 None:intergenic
GTTGTTCCTCATACTCATTC+TGG 0.307616 2_1:-78007834 Msa0227230:CDS
GATCGTTATAGGGTTGATAA+TGG 0.315135 2_1:-78007381 Msa0227230:CDS
GTGAGGGAAAATAAGAATTA+GGG 0.317479 2_1:-78007987 Msa0227230:five_prime_UTR
TTAAGAGGTTCATGTGTTAC+AGG 0.325552 2_1:-78005491 Msa0227230:three_prime_UTR
GAGAAATGGGACATGGATTT+GGG 0.327055 2_1:-78007104 Msa0227230:CDS
GTGTGGATGCAGAGACTCTT+TGG 0.328188 2_1:-78006390 Msa0227230:CDS
AGCAGCTCAAGCAGTGATTC+TGG 0.334206 2_1:-78005947 Msa0227230:CDS
CAACTATAACTGGTGGTTCT+TGG 0.335527 2_1:+78006166 None:intergenic
AAATTCGAGAGTCGATATAA+TGG 0.335951 2_1:-78005989 Msa0227230:CDS
TCAACAGTTGGATAGTGTTT+TGG 0.336808 2_1:-78007751 Msa0227230:CDS
CGCAAGCAAGAATTCCGAAT+TGG 0.349984 2_1:+78007194 None:intergenic
TTCAATTTCATCATCATGTT+GGG 0.352389 2_1:+78006426 None:intergenic
TGAGGTATGGCTTGGATTAT+GGG 0.352869 2_1:-78006741 Msa0227230:CDS
ATGTGTCTGTGGATCGTTAT+AGG 0.361266 2_1:-78007392 Msa0227230:CDS
GTGTTCCGAGAGAGGCTAAT+AGG 0.362707 2_1:-78007317 Msa0227230:CDS
AGTGATAGCTCCTCATCGTC+TGG 0.364047 2_1:-78005789 Msa0227230:CDS
CTCGGGGCATCAACTATAAC+TGG 0.373580 2_1:+78006156 None:intergenic
AGCGCAGCTTTGTCAGAATT+AGG 0.388451 2_1:-78005382 Msa0227230:three_prime_UTR
CATCTCACATCCGATGGACT+TGG 0.390431 2_1:-78006956 Msa0227230:CDS
GACTTCTTGTTACTCTCTGC+AGG 0.391383 2_1:+78007156 None:intergenic
GAAGCATGGCGTTGGAGAAA+TGG 0.393841 2_1:-78007118 Msa0227230:CDS
GGTTCCGGAGGATGGGAATT+CGG 0.396211 2_1:-78007730 Msa0227230:CDS
TACTGCTTACTATGCCAGTT+AGG 0.398204 2_1:-78005543 Msa0227230:three_prime_UTR
ATTGTAATTTGACTCTATAA+TGG 0.399524 2_1:+78006771 None:intergenic
AAACCTGCTTGTCTTTCTCT+CGG 0.407774 2_1:+78006138 None:intergenic
AACCTGCTTGTCTTTCTCTC+GGG 0.408916 2_1:+78006139 None:intergenic
GCCTCTTGACATGAGGAATC+TGG 0.411079 2_1:-78006662 Msa0227230:CDS
AGAGGATAACGTTAATTCCT+TGG 0.411103 2_1:-78007859 Msa0227230:CDS
CAACGGATGGCTGGGAGGTA+TGG 0.413417 2_1:-78007426 Msa0227230:CDS
TTAGGTTTACATCTACGTAT+TGG 0.416254 2_1:-78005525 Msa0227230:three_prime_UTR
TTATCAGGATCGTTTGCCTT+CGG 0.416620 2_1:+78006562 None:intergenic
TACGTAGATGTAAACCTAAC+TGG 0.417763 2_1:+78005529 None:intergenic
ACATCCTCCGCAAACTCCTT+TGG 0.418265 2_1:+78006892 None:intergenic
TTGGAATTTGGATATTTAAG+AGG 0.420791 2_1:-78005506 Msa0227230:three_prime_UTR
ACCGCCACCGTCACTGTTGA+TGG 0.421112 2_1:-78007804 Msa0227230:CDS
TCCCCATCCTTGCCCGTGCA+AGG 0.421675 2_1:-78006014 Msa0227230:CDS
CCCCTTGCACGGGCAAGGAT+GGG 0.422567 2_1:+78006012 None:intergenic
GAAGAGAAACAACGGATGGC+TGG 0.423094 2_1:-78007435 Msa0227230:CDS
GGTGAAACGGCACGCGTTTG+AGG 0.423664 2_1:+78008300 None:intergenic
GAATAGAACTGGTTCGCCTT+AGG 0.428319 2_1:+78007216 None:intergenic
CCCCATCCTTGCCCGTGCAA+GGG 0.431460 2_1:-78006013 Msa0227230:CDS
CTTCAATTTCATCATCATGT+TGG 0.431836 2_1:+78006425 None:intergenic
CCCTTGCACGGGCAAGGATG+GGG 0.435188 2_1:+78006013 None:intergenic
ATTCCGAATTGGAATAGAAC+TGG 0.439085 2_1:+78007205 None:intergenic
CAGCATTATTCGAGGAAAGA+TGG 0.443395 2_1:-78006795 Msa0227230:CDS
CTAGAAAGACTCCTGCTTTG+CGG 0.445924 2_1:-78006690 Msa0227230:CDS
GAGGAGATGTGATGATAAGA+AGG 0.445935 2_1:-78007574 Msa0227230:CDS
TCCCCTTGCACGGGCAAGGA+TGG 0.450565 2_1:+78006011 None:intergenic
GGATAACGGGAATAATGTTT+CGG 0.452791 2_1:-78007775 Msa0227230:CDS
GATCATTAAGAAACGAAATT+CGG 0.453860 2_1:-78006455 Msa0227230:CDS
CATCGTCTGGGTCTGATTCA+GGG 0.454571 2_1:-78005776 Msa0227230:CDS
ATCGTTATAGGGTTGATAAT+GGG 0.456646 2_1:-78007380 Msa0227230:CDS
CTCTCGAATTTCCCCTTGCA+CGG 0.458366 2_1:+78006001 None:intergenic
CACTCTGAGGTAGCTTCTGC+AGG 0.459504 2_1:-78007339 Msa0227230:CDS
AAGCATGGCGTTGGAGAAAT+GGG 0.460416 2_1:-78007117 Msa0227230:CDS
AGAGGCGGTGGCCGCAAAGC+AGG 0.462424 2_1:+78006679 None:intergenic
AACCCTAGATTTGAATTCTA+GGG 0.466617 2_1:-78008037 Msa0227230:intron
TAGTCTTGATGTTGTTCAGC+TGG 0.468758 2_1:-78007628 Msa0227230:CDS
ATTTGTTAGAACCAGCTTTG+AGG 0.470294 2_1:-78007593 Msa0227230:CDS
TACACGCCAGTTAAGTGTAA+TGG 0.471994 2_1:-78007292 Msa0227230:CDS
TTGATGGGACGAAGGATAAC+GGG 0.473241 2_1:-78007788 Msa0227230:CDS
CCATGACATATAATCCTAAA+GGG 0.476230 2_1:-78006849 Msa0227230:CDS
AATTCGAGAGTCGATATAAT+GGG 0.476726 2_1:-78005988 Msa0227230:CDS
CCCTTTAGGATTATATGTCA+TGG 0.477104 2_1:+78006849 None:intergenic
AGTCGAGGCTCAGCAAGAAT+TGG 0.481219 2_1:-78006924 Msa0227230:CDS
AGGAGATGTGATGATAAGAA+GGG 0.481966 2_1:-78007573 Msa0227230:CDS
CCAACGACCTCACCGTTTCA+AGG 0.483405 2_1:+78007469 None:intergenic
ACGGTCGAAGCAAGAGATGC+GGG 0.484337 2_1:-78007517 Msa0227230:CDS
GAACCAGTTCTATTCCAATT+CGG 0.484886 2_1:-78007208 Msa0227230:CDS
GCCATGACATATAATCCTAA+AGG 0.486072 2_1:-78006850 Msa0227230:CDS
TAATGTTTCGGTTCAACAGT+TGG 0.486868 2_1:-78007763 Msa0227230:CDS
TGTGGATGCAGAGACTCTTT+GGG 0.487754 2_1:-78006389 Msa0227230:CDS
GTCTCTGTGGAGCGGTGAAA+CGG 0.488084 2_1:+78008287 None:intergenic
GGGATTAAAAGGAAGCTTGA+GGG 0.488906 2_1:-78007495 Msa0227230:CDS
CCAGTGGATGTTGAACGTCT+TGG 0.491052 2_1:-78007000 Msa0227230:CDS
TCTAGCTTGAATAGCAAGTT+CGG 0.494718 2_1:+78006309 None:intergenic
TGTGTCTGTGGATCGTTATA+GGG 0.499515 2_1:-78007391 Msa0227230:CDS
TTAAGAATGGACTGGCTTCA+CGG 0.501932 2_1:-78007536 Msa0227230:CDS
CTCTCTCTAGAAACGGTGTA+TGG 0.502013 2_1:+78008265 None:intergenic
AATTAGTGAAGGTGTTGAGA+AGG 0.503461 2_1:-78007250 Msa0227230:CDS
TTGGAGAATGGTGTTAAGAA+TGG 0.503549 2_1:-78007549 Msa0227230:CDS
AATTTCCCCTTGCACGGGCA+AGG 0.506581 2_1:+78006007 None:intergenic
GTGAAACGGCACGCGTTTGA+GGG 0.507750 2_1:+78008301 None:intergenic
CTTCTGCTGGGCAATTCGCT+CGG 0.507968 2_1:+78006279 None:intergenic
TCATGAGATGAGGTATGGCT+TGG 0.513576 2_1:-78006749 Msa0227230:CDS
GTCAAGCTCAAGGGATTTGG+AGG 0.514668 2_1:+78006616 None:intergenic
TCCAACCTAGAATTCACAAC+TGG 0.516769 2_1:+78007702 None:intergenic
CATGGAAAGAAGCATGGCGT+TGG 0.520162 2_1:-78007126 Msa0227230:CDS
CCCATCCTTGCCCGTGCAAG+GGG 0.520316 2_1:-78006012 Msa0227230:CDS
ATCAACAGTGACGGTGGCGG+TGG 0.521030 2_1:+78007806 None:intergenic
GTTGATGGGACGAAGGATAA+CGG 0.523599 2_1:-78007789 Msa0227230:CDS
GCCAGTTGTGAATTCTAGGT+TGG 0.525168 2_1:-78007703 Msa0227230:CDS
GTCGAGTTTGAATCGTAGGC+AGG 0.526047 2_1:-78007661 Msa0227230:CDS
AGCATTATTCGAGGAAAGAT+GGG 0.527838 2_1:-78006794 Msa0227230:CDS
AGTGTAATGGTGTTAGAGAA+TGG 0.529484 2_1:-78007279 Msa0227230:CDS
TGATGAAACACAAGCACGGT+TGG 0.531315 2_1:-78007035 Msa0227230:CDS
TACAATCATGAGATGAGGTA+TGG 0.535160 2_1:-78006754 Msa0227230:CDS
AAGAGAAACAACGGATGGCT+GGG 0.537737 2_1:-78007434 Msa0227230:CDS
GCTCAAGGGATTTGGAGGGT+TGG 0.539474 2_1:+78006621 None:intergenic
GATAAGAAGGGGTTGGAGAA+TGG 0.540938 2_1:-78007561 Msa0227230:CDS
CAAATCACCAAAGGAGTTTG+CGG 0.541456 2_1:-78006899 Msa0227230:CDS
TCTCGAATTTCCCCTTGCAC+GGG 0.542840 2_1:+78006002 None:intergenic
ACCTGCTTGTCTTTCTCTCG+GGG 0.543462 2_1:+78006140 None:intergenic
GATGAAACACAAGCACGGTT+GGG 0.544423 2_1:-78007034 Msa0227230:CDS
AGGGCAAGATGTTCATGTAA+TGG 0.545167 2_1:-78006830 Msa0227230:CDS
CCAAGACGTTCAACATCCAC+TGG 0.547510 2_1:+78007000 None:intergenic
ATTCGTCGAGTTTGAATCGT+AGG 0.549038 2_1:-78007665 Msa0227230:CDS
TCATCACATCTCCTCAAAGC+TGG 0.550575 2_1:+78007582 None:intergenic
GTCTTTCTAGACAGAGGTGA+AGG 0.552966 2_1:+78006703 None:intergenic
GATGTTGTTCAGCTGGAAGA+TGG 0.557828 2_1:-78007621 Msa0227230:CDS
GAGCTTTCTCTCTCTAGAAA+CGG 0.558141 2_1:+78008258 None:intergenic
TTCCTCATGTCAAGAGGCGG+TGG 0.558440 2_1:+78006667 None:intergenic
ATCTCACATCCGATGGACTT+GGG 0.558638 2_1:-78006955 Msa0227230:CDS
GCTTGAGGGCGACCTTGAAA+CGG 0.560792 2_1:-78007481 Msa0227230:CDS
TATAGGGTTGATAATGGGAG+TGG 0.560910 2_1:-78007375 Msa0227230:CDS
TATTATCATCTCACATCCGA+TGG 0.564977 2_1:-78006962 Msa0227230:CDS
CGGATGGCTGGGAGGTATGG+TGG 0.565095 2_1:-78007423 Msa0227230:CDS
GAGATACAGAGATACTCGGA+GGG 0.565135 2_1:-78007915 Msa0227230:CDS
AAAGTTTGAGCAAATACAAG+AGG 0.565822 2_1:-78006336 Msa0227230:CDS
GTAGCACCAGAATGAGTATG+AGG 0.566136 2_1:+78007828 None:intergenic
GCCCCGAGAGAAAGACAAGC+AGG 0.567318 2_1:-78006141 Msa0227230:intron
GTAAACCTATTAGCCTCTCT+CGG 0.567513 2_1:+78007312 None:intergenic
CCGCCACCGTCACTGTTGAT+GGG 0.567667 2_1:-78007803 Msa0227230:CDS
GGAGCCGAATTCCCATCCTC+CGG 0.571712 2_1:+78007726 None:intergenic
ATGTGATGATAAGAAGGGGT+TGG 0.572103 2_1:-78007568 Msa0227230:CDS
ACATGATGATGAAATTGAAG+TGG 0.573976 2_1:-78006422 Msa0227230:CDS
CGAGCGAATTGCCCAGCAGA+AGG 0.576938 2_1:-78006278 Msa0227230:intron
GGGGACTGTAGCTGAAGATA+AGG 0.578014 2_1:-78007946 Msa0227230:CDS
TTGACATGAGGAATCTGGAT+AGG 0.578459 2_1:-78006657 Msa0227230:CDS
GTGATAGCTCCTCATCGTCT+GGG 0.578733 2_1:-78005788 Msa0227230:CDS
TTCTGCAGGTGTTCCGAGAG+AGG 0.582364 2_1:-78007325 Msa0227230:CDS
CCCATCAACAGTGACGGTGG+CGG 0.584798 2_1:+78007803 None:intergenic
CCTTGAAACGGTGAGGTCGT+TGG 0.585920 2_1:-78007469 Msa0227230:CDS
TGTGAATTCTAGGTTGGACG+TGG 0.587021 2_1:-78007697 Msa0227230:CDS
AGCTGAACGAGTCAAGCTCA+AGG 0.587721 2_1:+78006606 None:intergenic
TGAAGTGGACATTGATAGTG+TGG 0.589368 2_1:-78006407 Msa0227230:CDS
AGATGTTCATGTAATGGCGG+AGG 0.593716 2_1:-78006824 Msa0227230:CDS
GGTGAAGGTGCAGATATAGC+GGG 0.603194 2_1:+78006718 None:intergenic
GAAGGAGAAGAGAACACCTA+AGG 0.603536 2_1:-78007232 Msa0227230:CDS
AGGTGAAGGTGCAGATATAG+CGG 0.604052 2_1:+78006717 None:intergenic
GATTCTGGCTCTTCATCAAG+TGG 0.609326 2_1:-78005932 Msa0227230:intron
AACCTAGAATTCACAACTGG+CGG 0.610102 2_1:+78007705 None:intergenic
TAGAGAGATACAGAGATACT+CGG 0.610318 2_1:-78007919 Msa0227230:CDS
GGCGTTGGAGAAATGGGACA+TGG 0.610803 2_1:-78007111 Msa0227230:CDS
GGAGCTATCAGCATTATTCG+AGG 0.611908 2_1:-78006803 Msa0227230:CDS
CACGGTCGAAGCAAGAGATG+CGG 0.611992 2_1:-78007518 Msa0227230:CDS
TTCACAGTACCCAAGTCCAT+CGG 0.615883 2_1:+78006946 None:intergenic
GGTCGAAGCAAGAGATGCGG+GGG 0.619895 2_1:-78007515 Msa0227230:CDS
AATGGTCATGGAATTAGTGA+AGG 0.620816 2_1:-78007261 Msa0227230:CDS
AGTATGAGGAACAACTTCCA+AGG 0.625080 2_1:+78007842 None:intergenic
CAAATTACAATCATGAGATG+AGG 0.627329 2_1:-78006759 Msa0227230:CDS
GCTGAACGAGTCAAGCTCAA+GGG 0.627872 2_1:+78006607 None:intergenic
TCCAGATTCCTCATGTCAAG+AGG 0.627985 2_1:+78006661 None:intergenic
TTTGAGCAAATACAAGAGGA+AGG 0.629083 2_1:-78006332 Msa0227230:CDS
TTGGGTGTTTAATACTCCAG+TGG 0.632618 2_1:-78007016 Msa0227230:CDS
GGCCACCGCCTCTTGACATG+AGG 0.633195 2_1:-78006669 Msa0227230:CDS
GCAGGAGTCTTTCTAGACAG+AGG 0.633710 2_1:+78006697 None:intergenic
AAATCACATGGAAAGAAGCA+TGG 0.633912 2_1:-78007132 Msa0227230:CDS
CGGTCGAAGCAAGAGATGCG+GGG 0.634842 2_1:-78007516 Msa0227230:CDS
ATGGTGTTAGAGAATGGTCA+TGG 0.636665 2_1:-78007273 Msa0227230:CDS
TGGTGGTTTGAATGTGTCTG+TGG 0.636743 2_1:-78007403 Msa0227230:CDS
GTGAACCTAAATACCTCCCA+AGG 0.637851 2_1:-78005748 Msa0227230:exon
AACAAGAAGTCAAAATCACA+TGG 0.638541 2_1:-78007144 Msa0227230:CDS
AGCTAAGCACGCTCACTCTG+AGG 0.643578 2_1:-78007352 Msa0227230:CDS
TTCTTGTTACTCTCTGCAGG+CGG 0.647277 2_1:+78007159 None:intergenic
TCAAGCTCAAGGGATTTGGA+GGG 0.651277 2_1:+78006617 None:intergenic
CTAGATCTGATACAGAGTGA+AGG 0.652453 2_1:-78008190 Msa0227230:intron
CGTCCCATCAACAGTGACGG+TGG 0.653161 2_1:+78007800 None:intergenic
CTTCGTCCCATCAACAGTGA+CGG 0.654668 2_1:+78007797 None:intergenic
TGAATCAGACCCAGACGATG+AGG 0.655171 2_1:+78005779 None:intergenic
ATGGCTGGGAGGTATGGTGG+TGG 0.655927 2_1:-78007420 Msa0227230:CDS
GAAACGGTGTATGGTCTCTG+TGG 0.663908 2_1:+78008274 None:intergenic
GGGGATTAAAAGGAAGCTTG+AGG 0.665812 2_1:-78007496 Msa0227230:CDS
GAATGGTGTTAAGAATGGAC+TGG 0.666740 2_1:-78007544 Msa0227230:CDS
GCAAGATGTTCATGTAATGG+CGG 0.668019 2_1:-78006827 Msa0227230:CDS
CGTCACTGTTGATGGGACGA+AGG 0.671055 2_1:-78007796 Msa0227230:CDS
ATCACCAAAGGAGTTTGCGG+AGG 0.671626 2_1:-78006896 Msa0227230:CDS
AGATTCCTCATGTCAAGAGG+CGG 0.676274 2_1:+78006664 None:intergenic
TGTGTTATCTTCAAGATGTG+AGG 0.679433 2_1:-78005613 Msa0227230:three_prime_UTR
GATTGAAGAGAAACAACGGA+TGG 0.679746 2_1:-78007439 Msa0227230:CDS
GGGGCATCAACTATAACTGG+TGG 0.680239 2_1:+78006159 None:intergenic
GAATTGGTACAAATCACCAA+AGG 0.690101 2_1:-78006908 Msa0227230:CDS
ACTTGGGTACTGTGAAGTCG+AGG 0.692988 2_1:-78006939 Msa0227230:CDS
AGAAACAACGGATGGCTGGG+AGG 0.697772 2_1:-78007431 Msa0227230:CDS
AGGGCGACCTTGAAACGGTG+AGG 0.698598 2_1:-78007476 Msa0227230:CDS
AAGTTGATGAAACACAAGCA+CGG 0.699310 2_1:-78007039 Msa0227230:CDS
AGAGATACAGAGATACTCGG+AGG 0.700043 2_1:-78007916 Msa0227230:CDS
TTAGGATTGAAGAGAAACAA+CGG 0.702409 2_1:-78007443 Msa0227230:CDS
AGATACAGAGATACTCGGAG+GGG 0.714927 2_1:-78007914 Msa0227230:CDS
GGAGATGTGATGATAAGAAG+GGG 0.717368 2_1:-78007572 Msa0227230:CDS
GGTGTATGGTCTCTGTGGAG+CGG 0.730914 2_1:+78008279 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTGCAATTGAT+CGG - chr2_1:78005538-78005557 Msa0227230:three_prime_UTR 15.0%
!! AAGATGAATCAGAAAAAATT+AGG + chr2_1:78005572-78005591 None:intergenic 20.0%
!! AAAATTGAACAAATTAAACC+CGG + chr2_1:78005597-78005616 None:intergenic 20.0%
!! AAAAAATTTCAAAGTTTCAG+AGG - chr2_1:78005787-78005806 Msa0227230:CDS 20.0%
!! ATTGTAATTTGACTCTATAA+TGG + chr2_1:78006896-78006915 None:intergenic 20.0%
!!! GGGTTTAATTTGTTCAATTT+TGG - chr2_1:78005596-78005615 Msa0227230:three_prime_UTR 25.0%
! GATCATTAAGAAACGAAATT+CGG - chr2_1:78007209-78007228 Msa0227230:CDS 25.0%
! TTCAATTTCATCATCATGTT+GGG + chr2_1:78007241-78007260 None:intergenic 25.0%
! CCTTGTATAACCTAAATTAT+GGG + chr2_1:78007789-78007808 None:intergenic 25.0%
! ACCTTGTATAACCTAAATTA+TGG + chr2_1:78007790-78007809 None:intergenic 25.0%
! TTTGAATAAAAGTAAAAGCC+AGG + chr2_1:78007816-78007835 None:intergenic 25.0%
! CATATAACGAGATAAAAACT+TGG + chr2_1:78007956-78007975 None:intergenic 25.0%
!!! TGAATGCATTTCTATTTTTC+TGG - chr2_1:78008075-78008094 Msa0227230:intron 25.0%
! TTGGAATTTGGATATTTAAG+AGG - chr2_1:78008158-78008177 Msa0227230:intron 25.0%
!! AGATCAACTTTTATGCTTTT+AGG - chr2_1:78008207-78008226 Msa0227230:five_prime_UTR 25.0%
!! GATCAACTTTTATGCTTTTA+GGG - chr2_1:78008208-78008227 Msa0227230:five_prime_UTR 25.0%
! TTTTTCTGATTCATCTTCAC+CGG - chr2_1:78005575-78005594 Msa0227230:three_prime_UTR 30.0%
AAACCCTAGATTTGAATTCT+AGG - chr2_1:78005626-78005645 Msa0227230:three_prime_UTR 30.0%
AACCCTAGATTTGAATTCTA+GGG - chr2_1:78005627-78005646 Msa0227230:three_prime_UTR 30.0%
AACCCTAGAATTCAAATCTA+GGG + chr2_1:78005632-78005651 None:intergenic 30.0%
AAACCCTAGAATTCAAATCT+AGG + chr2_1:78005633-78005652 None:intergenic 30.0%
!!! TTTGAATTCTAGGGTTTTCT+AGG - chr2_1:78005636-78005655 Msa0227230:three_prime_UTR 30.0%
!!! TTGAATTCTAGGGTTTTCTA+GGG - chr2_1:78005637-78005656 Msa0227230:three_prime_UTR 30.0%
GTGAGGGAAAATAAGAATTA+GGG - chr2_1:78005677-78005696 Msa0227230:three_prime_UTR 30.0%
!!! GAATTAGGGTTTTGAATGAA+TGG - chr2_1:78005691-78005710 Msa0227230:three_prime_UTR 30.0%
TTAGGATTGAAGAGAAACAA+CGG - chr2_1:78006221-78006240 Msa0227230:intron 30.0%
!! ATCGTTATAGGGTTGATAAT+GGG - chr2_1:78006284-78006303 Msa0227230:CDS 30.0%
AACAAGAAGTCAAAATCACA+TGG - chr2_1:78006520-78006539 Msa0227230:CDS 30.0%
CCATGACATATAATCCTAAA+GGG - chr2_1:78006815-78006834 Msa0227230:CDS 30.0%
CAAATTACAATCATGAGATG+AGG - chr2_1:78006905-78006924 Msa0227230:CDS 30.0%
! TATGTCATGTCTCTTTTATC+AGG + chr2_1:78007120-78007139 None:intergenic 30.0%
!!! ACAATAGCATCTAGTTTTTC+AGG + chr2_1:78007186-78007205 None:intergenic 30.0%
CTTCAATTTCATCATCATGT+TGG + chr2_1:78007242-78007261 None:intergenic 30.0%
! ACATGATGATGAAATTGAAG+TGG - chr2_1:78007242-78007261 Msa0227230:CDS 30.0%
! GCTCAAACTTTTCTTATAGT+TGG + chr2_1:78007319-78007338 None:intergenic 30.0%
AAAGTTTGAGCAAATACAAG+AGG - chr2_1:78007328-78007347 Msa0227230:CDS 30.0%
TTAATATCCAGTGTCTTCTT+TGG - chr2_1:78007430-78007449 Msa0227230:CDS 30.0%
AAATTCGAGAGTCGATATAA+TGG - chr2_1:78007675-78007694 Msa0227230:CDS 30.0%
AATTCGAGAGTCGATATAAT+GGG - chr2_1:78007676-78007695 Msa0227230:CDS 30.0%
AGATATCTGTCCCATAATTT+AGG - chr2_1:78007776-78007795 Msa0227230:CDS 30.0%
! CCCATAATTTAGGTTATACA+AGG - chr2_1:78007786-78007805 Msa0227230:CDS 30.0%
! TTTTCTGCATCAAATTGTCT+GGG - chr2_1:78007997-78008016 Msa0227230:five_prime_UTR 30.0%
!!! GCTTTCTTTTGATTTCATAG+AGG - chr2_1:78008022-78008041 Msa0227230:five_prime_UTR 30.0%
TTAGGTTTACATCTACGTAT+TGG - chr2_1:78008139-78008158 Msa0227230:intron 30.0%
TACATCTACGTATTGGAATT+TGG - chr2_1:78008146-78008165 Msa0227230:intron 30.0%
! TTTTCTGATTCATCTTCACC+GGG - chr2_1:78005576-78005595 Msa0227230:three_prime_UTR 35.0%
!!! TAGGGTTTGGCTTTGATTTT+TGG - chr2_1:78005655-78005674 Msa0227230:three_prime_UTR 35.0%
!!! TTTGGCTTTGATTTTTGGTG+AGG - chr2_1:78005660-78005679 Msa0227230:three_prime_UTR 35.0%
!!! TTGGCTTTGATTTTTGGTGA+GGG - chr2_1:78005661-78005680 Msa0227230:three_prime_UTR 35.0%
GGTGAGGGAAAATAAGAATT+AGG - chr2_1:78005676-78005695 Msa0227230:three_prime_UTR 35.0%
!!! GGGTTTTGAATGAATGGATT+CGG - chr2_1:78005697-78005716 Msa0227230:three_prime_UTR 35.0%
!!! GGTTTTGAATGAATGGATTC+GGG - chr2_1:78005698-78005717 Msa0227230:three_prime_UTR 35.0%
!! GTTTTGAATGAATGGATTCG+GGG - chr2_1:78005699-78005718 Msa0227230:three_prime_UTR 35.0%
TAGAGAGATACAGAGATACT+CGG - chr2_1:78005745-78005764 Msa0227230:three_prime_UTR 35.0%
AGAGGATAACGTTAATTCCT+TGG - chr2_1:78005805-78005824 Msa0227230:intron 35.0%
!! GGATAACGGGAATAATGTTT+CGG - chr2_1:78005889-78005908 Msa0227230:intron 35.0%
TAATGTTTCGGTTCAACAGT+TGG - chr2_1:78005901-78005920 Msa0227230:intron 35.0%
! TCAACAGTTGGATAGTGTTT+TGG - chr2_1:78005913-78005932 Msa0227230:intron 35.0%
! ATTTGTTAGAACCAGCTTTG+AGG - chr2_1:78006071-78006090 Msa0227230:intron 35.0%
AGGAGATGTGATGATAAGAA+GGG - chr2_1:78006091-78006110 Msa0227230:intron 35.0%
! TTGGAGAATGGTGTTAAGAA+TGG - chr2_1:78006115-78006134 Msa0227230:intron 35.0%
!! GATCGTTATAGGGTTGATAA+TGG - chr2_1:78006283-78006302 Msa0227230:CDS 35.0%
CTAACACCATTACACTTAAC+TGG + chr2_1:78006381-78006400 None:intergenic 35.0%
! AGTGTAATGGTGTTAGAGAA+TGG - chr2_1:78006385-78006404 Msa0227230:CDS 35.0%
AATGGTCATGGAATTAGTGA+AGG - chr2_1:78006403-78006422 Msa0227230:CDS 35.0%
!! AATTAGTGAAGGTGTTGAGA+AGG - chr2_1:78006414-78006433 Msa0227230:CDS 35.0%
GAACCAGTTCTATTCCAATT+CGG - chr2_1:78006456-78006475 Msa0227230:CDS 35.0%
ATTCCGAATTGGAATAGAAC+TGG + chr2_1:78006462-78006481 None:intergenic 35.0%
AAATCACATGGAAAGAAGCA+TGG - chr2_1:78006532-78006551 Msa0227230:CDS 35.0%
!! AAGTTGATGAAACACAAGCA+CGG - chr2_1:78006625-78006644 Msa0227230:CDS 35.0%
TATTATCATCTCACATCCGA+TGG - chr2_1:78006702-78006721 Msa0227230:CDS 35.0%
GAATTGGTACAAATCACCAA+AGG - chr2_1:78006756-78006775 Msa0227230:CDS 35.0%
GCCATGACATATAATCCTAA+AGG - chr2_1:78006814-78006833 Msa0227230:CDS 35.0%
!! CCCTTTAGGATTATATGTCA+TGG + chr2_1:78006818-78006837 None:intergenic 35.0%
AGCATTATTCGAGGAAAGAT+GGG - chr2_1:78006870-78006889 Msa0227230:CDS 35.0%
TACAATCATGAGATGAGGTA+TGG - chr2_1:78006910-78006929 Msa0227230:CDS 35.0%
!!! ATAGCATCTAGTTTTTCAGG+TGG + chr2_1:78007183-78007202 None:intergenic 35.0%
TTTGAGCAAATACAAGAGGA+AGG - chr2_1:78007332-78007351 Msa0227230:CDS 35.0%
! TCTAGCTTGAATAGCAAGTT+CGG + chr2_1:78007358-78007377 None:intergenic 35.0%
! TGTCAATTTTACCTTCTGCT+GGG + chr2_1:78007400-78007419 None:intergenic 35.0%
! TTGTCAATTTTACCTTCTGC+TGG + chr2_1:78007401-78007420 None:intergenic 35.0%
TTATTTGCCAAAGAAGACAC+TGG + chr2_1:78007440-78007459 None:intergenic 35.0%
CAACAATAATAGTCCACAAG+AGG + chr2_1:78007554-78007573 None:intergenic 35.0%
!!! TTTTACCTTGGGAGGTATTT+AGG + chr2_1:78007924-78007943 None:intergenic 35.0%
! GGAGTTTGTGATTTTACCTT+GGG + chr2_1:78007935-78007954 None:intergenic 35.0%
! TGGAGTTTGTGATTTTACCT+TGG + chr2_1:78007936-78007955 None:intergenic 35.0%
! GTTTTCTGCATCAAATTGTC+TGG - chr2_1:78007996-78008015 Msa0227230:five_prime_UTR 35.0%
TGTGTTATCTTCAAGATGTG+AGG - chr2_1:78008051-78008070 Msa0227230:intron 35.0%
!! TTCTATTTTTCTGGTGCACA+AGG - chr2_1:78008084-78008103 Msa0227230:intron 35.0%
TACGTAGATGTAAACCTAAC+TGG + chr2_1:78008138-78008157 None:intergenic 35.0%
TTAAGAGGTTCATGTGTTAC+AGG - chr2_1:78008173-78008192 Msa0227230:intron 35.0%
! GAGCTTTCTCTCTCTAGAAA+CGG + chr2_1:78005409-78005428 None:intergenic 40.0%
CTAGATCTGATACAGAGTGA+AGG - chr2_1:78005474-78005493 Msa0227230:three_prime_UTR 40.0%
!!! TTCTAGGGTTTTCTAGGGTT+TGG - chr2_1:78005642-78005661 Msa0227230:three_prime_UTR 40.0%
AGTATGAGGAACAACTTCCA+AGG + chr2_1:78005825-78005844 None:intergenic 40.0%
GTTGTTCCTCATACTCATTC+TGG - chr2_1:78005830-78005849 Msa0227230:intron 40.0%
!!! GTTGGATAGTGTTTTGGTTC+CGG - chr2_1:78005919-78005938 Msa0227230:intron 40.0%
AACCTAGAATTCACAACTGG+CGG + chr2_1:78005962-78005981 None:intergenic 40.0%
TCCAACCTAGAATTCACAAC+TGG + chr2_1:78005965-78005984 None:intergenic 40.0%
ATTCGTCGAGTTTGAATCGT+AGG - chr2_1:78005999-78006018 Msa0227230:CDS 40.0%
! TAGTCTTGATGTTGTTCAGC+TGG - chr2_1:78006036-78006055 Msa0227230:intron 40.0%
GAGGAGATGTGATGATAAGA+AGG - chr2_1:78006090-78006109 Msa0227230:intron 40.0%
GGAGATGTGATGATAAGAAG+GGG - chr2_1:78006092-78006111 Msa0227230:intron 40.0%
ATGTGATGATAAGAAGGGGT+TGG - chr2_1:78006096-78006115 Msa0227230:intron 40.0%
! GAATGGTGTTAAGAATGGAC+TGG - chr2_1:78006120-78006139 Msa0227230:intron 40.0%
!! TTAAGAATGGACTGGCTTCA+CGG - chr2_1:78006128-78006147 Msa0227230:intron 40.0%
GGGATTAAAAGGAAGCTTGA+GGG - chr2_1:78006169-78006188 Msa0227230:CDS 40.0%
GATTGAAGAGAAACAACGGA+TGG - chr2_1:78006225-78006244 Msa0227230:intron 40.0%
ATGTGTCTGTGGATCGTTAT+AGG - chr2_1:78006272-78006291 Msa0227230:intron 40.0%
TGTGTCTGTGGATCGTTATA+GGG - chr2_1:78006273-78006292 Msa0227230:intron 40.0%
!! TATAGGGTTGATAATGGGAG+TGG - chr2_1:78006289-78006308 Msa0227230:CDS 40.0%
! GTAAACCTATTAGCCTCTCT+CGG + chr2_1:78006355-78006374 None:intergenic 40.0%
TACACGCCAGTTAAGTGTAA+TGG - chr2_1:78006372-78006391 Msa0227230:CDS 40.0%
! ATGGTGTTAGAGAATGGTCA+TGG - chr2_1:78006391-78006410 Msa0227230:CDS 40.0%
GAGAAATGGGACATGGATTT+GGG - chr2_1:78006560-78006579 Msa0227230:CDS 40.0%
TTGGGTGTTTAATACTCCAG+TGG - chr2_1:78006648-78006667 Msa0227230:CDS 40.0%
CAAATCACCAAAGGAGTTTG+CGG - chr2_1:78006765-78006784 Msa0227230:CDS 40.0%
ACATGAACATCTTGCCCTTT+AGG + chr2_1:78006832-78006851 None:intergenic 40.0%
AGGGCAAGATGTTCATGTAA+TGG - chr2_1:78006834-78006853 Msa0227230:CDS 40.0%
GCAAGATGTTCATGTAATGG+CGG - chr2_1:78006837-78006856 Msa0227230:CDS 40.0%
CAGCATTATTCGAGGAAAGA+TGG - chr2_1:78006869-78006888 Msa0227230:CDS 40.0%
! ATGAGGTATGGCTTGGATTA+TGG - chr2_1:78006922-78006941 Msa0227230:CDS 40.0%
! TGAGGTATGGCTTGGATTAT+GGG - chr2_1:78006923-78006942 Msa0227230:CDS 40.0%
TTGACATGAGGAATCTGGAT+AGG - chr2_1:78007007-78007026 Msa0227230:CDS 40.0%
TTATCAGGATCGTTTGCCTT+CGG + chr2_1:78007105-78007124 None:intergenic 40.0%
!! TGAAGTGGACATTGATAGTG+TGG - chr2_1:78007257-78007276 Msa0227230:CDS 40.0%
CAACTATAACTGGTGGTTCT+TGG + chr2_1:78007501-78007520 None:intergenic 40.0%
AAACCTGCTTGTCTTTCTCT+CGG + chr2_1:78007529-78007548 None:intergenic 40.0%
TAGGTTATACAAGGTCAACC+TGG - chr2_1:78007795-78007814 Msa0227230:CDS 40.0%
! GTTTGTGATTTTACCTTGGG+AGG + chr2_1:78007932-78007951 None:intergenic 40.0%
TACTGCTTACTATGCCAGTT+AGG - chr2_1:78008121-78008140 Msa0227230:intron 40.0%
! CTCTCTCTAGAAACGGTGTA+TGG + chr2_1:78005402-78005421 None:intergenic 45.0%
AGAGATACAGAGATACTCGG+AGG - chr2_1:78005748-78005767 Msa0227230:exon 45.0%
GAGATACAGAGATACTCGGA+GGG - chr2_1:78005749-78005768 Msa0227230:exon 45.0%
AGATACAGAGATACTCGGAG+GGG - chr2_1:78005750-78005769 Msa0227230:exon 45.0%
GTAGCACCAGAATGAGTATG+AGG + chr2_1:78005839-78005858 None:intergenic 45.0%
! GTTGATGGGACGAAGGATAA+CGG - chr2_1:78005875-78005894 Msa0227230:intron 45.0%
TTGATGGGACGAAGGATAAC+GGG - chr2_1:78005876-78005895 Msa0227230:intron 45.0%
GCCAGTTGTGAATTCTAGGT+TGG - chr2_1:78005961-78005980 Msa0227230:CDS 45.0%
! TGTGAATTCTAGGTTGGACG+TGG - chr2_1:78005967-78005986 Msa0227230:CDS 45.0%
GATGTTGTTCAGCTGGAAGA+TGG - chr2_1:78006043-78006062 Msa0227230:intron 45.0%
TCATCACATCTCCTCAAAGC+TGG + chr2_1:78006085-78006104 None:intergenic 45.0%
GATAAGAAGGGGTTGGAGAA+TGG - chr2_1:78006103-78006122 Msa0227230:intron 45.0%
AAGAGATGCGGGGGATTAAA+AGG - chr2_1:78006158-78006177 Msa0227230:CDS 45.0%
GGGGATTAAAAGGAAGCTTG+AGG - chr2_1:78006168-78006187 Msa0227230:CDS 45.0%
AAGAGAAACAACGGATGGCT+GGG - chr2_1:78006230-78006249 Msa0227230:intron 45.0%
TGGTGGTTTGAATGTGTCTG+TGG - chr2_1:78006261-78006280 Msa0227230:intron 45.0%
GAAGGAGAAGAGAACACCTA+AGG - chr2_1:78006432-78006451 Msa0227230:CDS 45.0%
!! GAATAGAACTGGTTCGCCTT+AGG + chr2_1:78006451-78006470 None:intergenic 45.0%
CGCAAGCAAGAATTCCGAAT+TGG + chr2_1:78006473-78006492 None:intergenic 45.0%
TTCTTGTTACTCTCTGCAGG+CGG + chr2_1:78006508-78006527 None:intergenic 45.0%
GACTTCTTGTTACTCTCTGC+AGG + chr2_1:78006511-78006530 None:intergenic 45.0%
! AAGCATGGCGTTGGAGAAAT+GGG - chr2_1:78006547-78006566 Msa0227230:CDS 45.0%
GGAGAAATGGGACATGGATT+TGG - chr2_1:78006559-78006578 Msa0227230:CDS 45.0%
TGATGAAACACAAGCACGGT+TGG - chr2_1:78006629-78006648 Msa0227230:CDS 45.0%
GATGAAACACAAGCACGGTT+GGG - chr2_1:78006630-78006649 Msa0227230:CDS 45.0%
! ATCTCACATCCGATGGACTT+GGG - chr2_1:78006709-78006728 Msa0227230:CDS 45.0%
TTCACAGTACCCAAGTCCAT+CGG + chr2_1:78006721-78006740 None:intergenic 45.0%
AGATGTTCATGTAATGGCGG+AGG - chr2_1:78006840-78006859 Msa0227230:CDS 45.0%
GGAGCTATCAGCATTATTCG+AGG - chr2_1:78006861-78006880 Msa0227230:CDS 45.0%
TCATGAGATGAGGTATGGCT+TGG - chr2_1:78006915-78006934 Msa0227230:CDS 45.0%
! AGGTGAAGGTGCAGATATAG+CGG + chr2_1:78006950-78006969 None:intergenic 45.0%
GTCTTTCTAGACAGAGGTGA+AGG + chr2_1:78006964-78006983 None:intergenic 45.0%
CTAGAAAGACTCCTGCTTTG+CGG - chr2_1:78006974-78006993 Msa0227230:CDS 45.0%
AGATTCCTCATGTCAAGAGG+CGG + chr2_1:78007003-78007022 None:intergenic 45.0%
TCCAGATTCCTCATGTCAAG+AGG + chr2_1:78007006-78007025 None:intergenic 45.0%
TCAAGCTCAAGGGATTTGGA+GGG + chr2_1:78007050-78007069 None:intergenic 45.0%
!!! TCGTTTGCCTTCGGCTTTTT+TGG + chr2_1:78007096-78007115 None:intergenic 45.0%
TGTGGATGCAGAGACTCTTT+GGG - chr2_1:78007275-78007294 Msa0227230:CDS 45.0%
AACCTGCTTGTCTTTCTCTC+GGG + chr2_1:78007528-78007547 None:intergenic 45.0%
GAGAGAAAGACAAGCAGGTT+TGG - chr2_1:78007528-78007547 Msa0227230:CDS 45.0%
!! GATTCTGGCTCTTCATCAAG+TGG - chr2_1:78007732-78007751 Msa0227230:CDS 45.0%
GTGAACCTAAATACCTCCCA+AGG - chr2_1:78007916-78007935 Msa0227230:CDS 45.0%
! AGCGCAGCTTTGTCAGAATT+AGG - chr2_1:78008282-78008301 Msa0227230:five_prime_UTR 45.0%
GAAACGGTGTATGGTCTCTG+TGG + chr2_1:78005393-78005412 None:intergenic 50.0%
TCTCTCTCTCTCTCTCTCTC+TGG + chr2_1:78005443-78005462 None:intergenic 50.0%
GGGGACTGTAGCTGAAGATA+AGG - chr2_1:78005718-78005737 Msa0227230:three_prime_UTR 50.0%
CTTCGTCCCATCAACAGTGA+CGG + chr2_1:78005870-78005889 None:intergenic 50.0%
!!! GGATAGTGTTTTGGTTCCGG+AGG - chr2_1:78005922-78005941 Msa0227230:intron 50.0%
!!! AGTGTTTTGGTTCCGGAGGA+TGG - chr2_1:78005926-78005945 Msa0227230:intron 50.0%
!!! GTGTTTTGGTTCCGGAGGAT+GGG - chr2_1:78005927-78005946 Msa0227230:intron 50.0%
CTCCGCCAGTTGTGAATTCT+AGG - chr2_1:78005957-78005976 Msa0227230:CDS 50.0%
GTCGAGTTTGAATCGTAGGC+AGG - chr2_1:78006003-78006022 Msa0227230:CDS 50.0%
GAAGAGAAACAACGGATGGC+TGG - chr2_1:78006229-78006248 Msa0227230:intron 50.0%
GTGTTCCGAGAGAGGCTAAT+AGG - chr2_1:78006347-78006366 Msa0227230:CDS 50.0%
! CATGGAAAGAAGCATGGCGT+TGG - chr2_1:78006538-78006557 Msa0227230:CDS 50.0%
! GAAGCATGGCGTTGGAGAAA+TGG - chr2_1:78006546-78006565 Msa0227230:CDS 50.0%
CCAAGACGTTCAACATCCAC+TGG + chr2_1:78006667-78006686 None:intergenic 50.0%
CCAGTGGATGTTGAACGTCT+TGG - chr2_1:78006664-78006683 Msa0227230:CDS 50.0%
! CATCTCACATCCGATGGACT+TGG - chr2_1:78006708-78006727 Msa0227230:CDS 50.0%
! ACTTGGGTACTGTGAAGTCG+AGG - chr2_1:78006725-78006744 Msa0227230:CDS 50.0%
AGTCGAGGCTCAGCAAGAAT+TGG - chr2_1:78006740-78006759 Msa0227230:CDS 50.0%
ATCACCAAAGGAGTTTGCGG+AGG - chr2_1:78006768-78006787 Msa0227230:CDS 50.0%
ACATCCTCCGCAAACTCCTT+TGG + chr2_1:78006775-78006794 None:intergenic 50.0%
! GGTGAAGGTGCAGATATAGC+GGG + chr2_1:78006949-78006968 None:intergenic 50.0%
GCAGGAGTCTTTCTAGACAG+AGG + chr2_1:78006970-78006989 None:intergenic 50.0%
GCCTCTTGACATGAGGAATC+TGG - chr2_1:78007002-78007021 Msa0227230:CDS 50.0%
GTCAAGCTCAAGGGATTTGG+AGG + chr2_1:78007051-78007070 None:intergenic 50.0%
CGAGTCAAGCTCAAGGGATT+TGG + chr2_1:78007054-78007073 None:intergenic 50.0%
GCTGAACGAGTCAAGCTCAA+GGG + chr2_1:78007060-78007079 None:intergenic 50.0%
AGCTGAACGAGTCAAGCTCA+AGG + chr2_1:78007061-78007080 None:intergenic 50.0%
TCCTGCTCCAAAAAAGCCGA+AGG - chr2_1:78007086-78007105 Msa0227230:CDS 50.0%
GTGTGGATGCAGAGACTCTT+TGG - chr2_1:78007274-78007293 Msa0227230:CDS 50.0%
! GGGGCATCAACTATAACTGG+TGG + chr2_1:78007508-78007527 None:intergenic 50.0%
! CTCGGGGCATCAACTATAAC+TGG + chr2_1:78007511-78007530 None:intergenic 50.0%
ACCTGCTTGTCTTTCTCTCG+GGG + chr2_1:78007527-78007546 None:intergenic 50.0%
TCTCGAATTTCCCCTTGCAC+GGG + chr2_1:78007665-78007684 None:intergenic 50.0%
CTCTCGAATTTCCCCTTGCA+CGG + chr2_1:78007666-78007685 None:intergenic 50.0%
AGCAGCTCAAGCAGTGATTC+TGG - chr2_1:78007717-78007736 Msa0227230:CDS 50.0%
AGTGATAGCTCCTCATCGTC+TGG - chr2_1:78007875-78007894 Msa0227230:CDS 50.0%
GTGATAGCTCCTCATCGTCT+GGG - chr2_1:78007876-78007895 Msa0227230:CDS 50.0%
TGAATCAGACCCAGACGATG+AGG + chr2_1:78007888-78007907 None:intergenic 50.0%
TCATCGTCTGGGTCTGATTC+AGG - chr2_1:78007887-78007906 Msa0227230:CDS 50.0%
CATCGTCTGGGTCTGATTCA+GGG - chr2_1:78007888-78007907 Msa0227230:CDS 50.0%
GTGAAACGGCACGCGTTTGA+GGG + chr2_1:78005366-78005385 None:intergenic 55.0%
GTCTCTGTGGAGCGGTGAAA+CGG + chr2_1:78005380-78005399 None:intergenic 55.0%
GGTGTATGGTCTCTGTGGAG+CGG + chr2_1:78005388-78005407 None:intergenic 55.0%
! CGTCACTGTTGATGGGACGA+AGG - chr2_1:78005868-78005887 Msa0227230:intron 55.0%
GGTTCCGGAGGATGGGAATT+CGG - chr2_1:78005934-78005953 Msa0227230:CDS 55.0%
CACGGTCGAAGCAAGAGATG+CGG - chr2_1:78006146-78006165 Msa0227230:CDS 55.0%
ACGGTCGAAGCAAGAGATGC+GGG - chr2_1:78006147-78006166 Msa0227230:CDS 55.0%
GCTTGAGGGCGACCTTGAAA+CGG - chr2_1:78006183-78006202 Msa0227230:intron 55.0%
CCAACGACCTCACCGTTTCA+AGG + chr2_1:78006198-78006217 None:intergenic 55.0%
CCTTGAAACGGTGAGGTCGT+TGG - chr2_1:78006195-78006214 Msa0227230:intron 55.0%
!! CGGTGAGGTCGTTGGTGATT+AGG - chr2_1:78006203-78006222 Msa0227230:intron 55.0%
AGAAACAACGGATGGCTGGG+AGG - chr2_1:78006233-78006252 Msa0227230:intron 55.0%
AGCTAAGCACGCTCACTCTG+AGG - chr2_1:78006312-78006331 Msa0227230:CDS 55.0%
!! CACTCTGAGGTAGCTTCTGC+AGG - chr2_1:78006325-78006344 Msa0227230:CDS 55.0%
TTCTGCAGGTGTTCCGAGAG+AGG - chr2_1:78006339-78006358 Msa0227230:CDS 55.0%
! GGCGTTGGAGAAATGGGACA+TGG - chr2_1:78006553-78006572 Msa0227230:CDS 55.0%
TTCCTCATGTCAAGAGGCGG+TGG + chr2_1:78007000-78007019 None:intergenic 55.0%
GCTCAAGGGATTTGGAGGGT+TGG + chr2_1:78007046-78007065 None:intergenic 55.0%
!!! GCCTTCGGCTTTTTTGGAGC+AGG + chr2_1:78007090-78007109 None:intergenic 55.0%
CTTCTGCTGGGCAATTCGCT+CGG + chr2_1:78007388-78007407 None:intergenic 55.0%
!! CTGGTGGTTCTTGGCTCTAC+AGG + chr2_1:78007492-78007511 None:intergenic 55.0%
CAAGCAGGTTTGGCCTCTTG+TGG - chr2_1:78007538-78007557 Msa0227230:CDS 55.0%
AATTTCCCCTTGCACGGGCA+AGG + chr2_1:78007660-78007679 None:intergenic 55.0%
GGTGAAACGGCACGCGTTTG+AGG + chr2_1:78005367-78005386 None:intergenic 60.0%
! ATCAACAGTGACGGTGGCGG+TGG + chr2_1:78005861-78005880 None:intergenic 60.0%
ACCGCCACCGTCACTGTTGA+TGG - chr2_1:78005860-78005879 Msa0227230:intron 60.0%
CCCATCAACAGTGACGGTGG+CGG + chr2_1:78005864-78005883 None:intergenic 60.0%
CCGCCACCGTCACTGTTGAT+GGG - chr2_1:78005861-78005880 Msa0227230:intron 60.0%
CGTCCCATCAACAGTGACGG+TGG + chr2_1:78005867-78005886 None:intergenic 60.0%
GGAGCCGAATTCCCATCCTC+CGG + chr2_1:78005941-78005960 None:intergenic 60.0%
CGGTCGAAGCAAGAGATGCG+GGG - chr2_1:78006148-78006167 Msa0227230:CDS 60.0%
GGTCGAAGCAAGAGATGCGG+GGG - chr2_1:78006149-78006168 Msa0227230:CDS 60.0%
AGGGCGACCTTGAAACGGTG+AGG - chr2_1:78006188-78006207 Msa0227230:intron 60.0%
CAACGGATGGCTGGGAGGTA+TGG - chr2_1:78006238-78006257 Msa0227230:intron 60.0%
! ATGGCTGGGAGGTATGGTGG+TGG - chr2_1:78006244-78006263 Msa0227230:intron 60.0%
CGAGCGAATTGCCCAGCAGA+AGG - chr2_1:78007386-78007405 Msa0227230:CDS 60.0%
GCCCCGAGAGAAAGACAAGC+AGG - chr2_1:78007523-78007542 Msa0227230:CDS 60.0%
! CGGATGGCTGGGAGGTATGG+TGG - chr2_1:78006241-78006260 Msa0227230:intron 65.0%
GGCCACCGCCTCTTGACATG+AGG - chr2_1:78006995-78007014 Msa0227230:CDS 65.0%
TCCCCATCCTTGCCCGTGCA+AGG - chr2_1:78007650-78007669 Msa0227230:CDS 65.0%
CCCTTGCACGGGCAAGGATG+GGG + chr2_1:78007654-78007673 None:intergenic 65.0%
CCCCATCCTTGCCCGTGCAA+GGG - chr2_1:78007651-78007670 Msa0227230:CDS 65.0%
CCCCTTGCACGGGCAAGGAT+GGG + chr2_1:78007655-78007674 None:intergenic 65.0%
CCCATCCTTGCCCGTGCAAG+GGG - chr2_1:78007652-78007671 Msa0227230:CDS 65.0%
TCCCCTTGCACGGGCAAGGA+TGG + chr2_1:78007656-78007675 None:intergenic 65.0%
AGAGGCGGTGGCCGCAAAGC+AGG + chr2_1:78006988-78007007 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 78005362 78008324 78005362 ID=Msa0227230;Name=Msa0227230
chr2_1 mRNA 78005362 78008324 78005362 ID=Msa0227230-mRNA-1;Parent=Msa0227230;Name=Msa0227230-mRNA-1;_AED=0.03;_eAED=0.03;_QI=209|1|1|1|0.75|0.6|5|405|638
chr2_1 exon 78005362 78005819 78005362 ID=Msa0227230-mRNA-1:exon:23776;Parent=Msa0227230-mRNA-1
chr2_1 exon 78005933 78006049 78005933 ID=Msa0227230-mRNA-1:exon:23775;Parent=Msa0227230-mRNA-1
chr2_1 exon 78006142 78006190 78006142 ID=Msa0227230-mRNA-1:exon:23774;Parent=Msa0227230-mRNA-1
chr2_1 exon 78006279 78008051 78006279 ID=Msa0227230-mRNA-1:exon:23773;Parent=Msa0227230-mRNA-1
chr2_1 exon 78008191 78008324 78008191 ID=Msa0227230-mRNA-1:exon:23772;Parent=Msa0227230-mRNA-1
chr2_1 five_prime_UTR 78008191 78008324 78008191 ID=Msa0227230-mRNA-1:five_prime_utr;Parent=Msa0227230-mRNA-1
chr2_1 five_prime_UTR 78007977 78008051 78007977 ID=Msa0227230-mRNA-1:five_prime_utr;Parent=Msa0227230-mRNA-1
chr2_1 CDS 78006279 78007976 78006279 ID=Msa0227230-mRNA-1:cds;Parent=Msa0227230-mRNA-1
chr2_1 CDS 78006142 78006190 78006142 ID=Msa0227230-mRNA-1:cds;Parent=Msa0227230-mRNA-1
chr2_1 CDS 78005933 78006049 78005933 ID=Msa0227230-mRNA-1:cds;Parent=Msa0227230-mRNA-1
chr2_1 CDS 78005767 78005819 78005767 ID=Msa0227230-mRNA-1:cds;Parent=Msa0227230-mRNA-1
chr2_1 three_prime_UTR 78005362 78005766 78005362 ID=Msa0227230-mRNA-1:three_prime_utr;Parent=Msa0227230-mRNA-1
Gene Sequence

>Msa0227230

ATGGATTCGGGGACTGTAGCTGAAGATAAGGTAGTTAGAGAGATACAGAGATACTCGGAGGGGAAAGTATACACGAGAAAAAATTTCAAAGTTTCAGAGGATAACGTTAATTCCTTGGAAGTTGTTCCTCATACTCATTCTGGTGCTACCACCGCCACCGTCACTGTTGATGGGACGAAGGATAACGGGAATAATGTTTCGGTTCAACAGTTGGATAGTGTTTTGGTTCCGGAGGATGGGAATTCGGCTCCGCCAGTTGTGAATTCTAGGTTGGACGTGGTTTCTGAAGATTCGTCGAGTTTGAATCGTAGGCAGGATGAACATTTTAGTCTTGATGTTGTTCAGCTGGAAGATGGTAATGATTTGTTAGAACCAGCTTTGAGGAGATGTGATGATAAGAAGGGGTTGGAGAATGGTGTTAAGAATGGACTGGCTTCACGGTCGAAGCAAGAGATGCGGGGGATTAAAAGGAAGCTTGAGGGCGACCTTGAAACGGTGAGGTCGTTGGTGATTAGGATTGAAGAGAAACAACGGATGGCTGGGAGGTATGGTGGTGGTTTGAATGTGTCTGTGGATCGTTATAGGGTTGATAATGGGAGTGGAGCTAAGCACGCTCACTCTGAGGTAGCTTCTGCAGGTGTTCCGAGAGAGGCTAATAGGTTTACACGCCAGTTAAGTGTAATGGTGTTAGAGAATGGTCATGGAATTAGTGAAGGTGTTGAGAAGGAGAAGAGAACACCTAAGGCGAACCAGTTCTATTCCAATTCGGAATTCTTGCTTGCGAAAGATAAGTTTCCGCCTGCAGAGAGTAACAAGAAGTCAAAATCACATGGAAAGAAGCATGGCGTTGGAGAAATGGGACATGGATTTGGGATAGTATCGAAGTATTTGAAGAACTGTAGCTCATTGCTTGAAAAGTTGATGAAACACAAGCACGGTTGGGTGTTTAATACTCCAGTGGATGTTGAACGTCTTGGTTTGCATGATTATTTTATTATCATCTCACATCCGATGGACTTGGGTACTGTGAAGTCGAGGCTCAGCAAGAATTGGTACAAATCACCAAAGGAGTTTGCGGAGGATGTGAGACTTACTTTTCGTAACGCCATGACATATAATCCTAAAGGGCAAGATGTTCATGTAATGGCGGAGGAGCTATCAGCATTATTCGAGGAAAGATGGGCCATTATAGAGTCAAATTACAATCATGAGATGAGGTATGGCTTGGATTATGGGCCCGCTATATCTGCACCTTCACCTCTGTCTAGAAAGACTCCTGCTTTGCGGCCACCGCCTCTTGACATGAGGAATCTGGATAGGTCAGAATCAATAACCAACCCTCCAAATCCCTTGAGCTTGACTCGTTCAGCTCGAACTCCTGCTCCAAAAAAGCCGAAGGCAAACGATCCTGATAAAAGAGACATGACATACGATGAAAAGCAAAAACTTAGCACACATCTTCAGAATCTACCACCTGAAAAACTAGATGCTATTGTACAGATCATTAAGAAACGAAATTCGGCACTTTCCCAACATGATGATGAAATTGAAGTGGACATTGATAGTGTGGATGCAGAGACTCTTTGGGAGCTTGATAGATATGTTACCAACTATAAGAAAAGTTTGAGCAAATACAAGAGGAAGGCCGAACTTGCTATTCAAGCTAGAGCAGAAGCCGAGCGAATTGCCCAGCAGAAGAGCCAAGAACCACCAGTTATAGTTGATGCCCCGAGAGAAAGACAAGCAGATGAAAGAAATGCTCCCCATCCTTGCCCGTGCAAGGGGAAATTCGAGAGTCGATATAATGGGAGTAAGACAAGTAGCTCAAGCAGCTCAAGCAGTGATTCTGGCTCTTCATCAAGTGATTCTGATAGTGATAGCTCCTCATCGTCTGGGTCTGATTCAGGGTCACAGTGA
Protein sequence

>Msa0227230

MDSGTVAEDKVVREIQRYSEGKVYTRKNFKVSEDNVNSLEVVPHTHSGATTATVTVDGTKDNGNNVSVQQLDSVLVPEDGNSAPPVVNSRLDVVSEDSSSLNRRQDEHFSLDVVQLEDGNDLLEPALRRCDDKKGLENGVKNGLASRSKQEMRGIKRKLEGDLETVRSLVIRIEEKQRMAGRYGGGLNVSVDRYRVDNGSGAKHAHSEVASAGVPREANRFTRQLSVMVLENGHGISEGVEKEKRTPKANQFYSNSEFLLAKDKFPPAESNKKSKSHGKKHGVGEMGHGFGIVSKYLKNCSSLLEKLMKHKHGWVFNTPVDVERLGLHDYFIIISHPMDLGTVKSRLSKNWYKSPKEFAEDVRLTFRNAMTYNPKGQDVHVMAEELSALFEERWAIIESNYNHEMRYGLDYGPAISAPSPLSRKTPALRPPPLDMRNLDRSESITNPPNPLSLTRSARTPAPKKPKANDPDKRDMTYDEKQKLSTHLQNLPPEKLDAIVQIIKKRNSALSQHDDEIEVDIDSVDAETLWELDRYVTNYKKSLSKYKRKAELAIQARAEAERIAQQKSQEPPVIVDAPRERQADERNAPHPCPCKGKFESRYNGSKTSSSSSSSSDSGSSSSDSDSDSSSSSGSDSGSQ*