AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0839000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0839000 MtrunA17_Chr5g0407081 96.501 543 18 1 1 543 1 542 0.0 1069
Msa0839000 MtrunA17_Chr5g0408021 85.714 91 13 0 453 543 5 95 2.60e-44 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0839000 AT2G17550.1 38.246 570 225 24 1 543 1 470 7.53e-79 263
Msa0839000 AT2G17550.2 38.306 496 180 23 74 543 1 396 1.94e-63 220

Find 104 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAAGAATAAGTGAAAATA+TGG 0.225343 5_4:-13606822 Msa0839000:CDS
GTTATAAGATTTGATCATTA+AGG 0.248170 5_4:+13606406 None:intergenic
TTTGTGAGCATGGACTTTCT+TGG 0.293337 5_4:+13607805 None:intergenic
TGAATCTCTACTGATAAATT+CGG 0.305013 5_4:+13606007 None:intergenic
AAAATCATCATACCAATAAA+TGG 0.305159 5_4:+13608608 None:intergenic
AAATACAAACAGGATCTTTC+AGG 0.313229 5_4:-13605842 Msa0839000:CDS
AGCAAAACTGTATCTCAAAT+TGG 0.317168 5_4:+13605581 None:intergenic
GCTAGAAACACTGCCGATTT+CGG 0.329590 5_4:+13606547 None:intergenic
GAAATGAGTCGAATTCATTA+TGG 0.333811 5_4:+13606723 None:intergenic
GATGGATGGAGCCAGAGTTT+TGG 0.337357 5_4:-13606674 Msa0839000:CDS
ATTTGACGAGCAATAAGTTT+AGG 0.338979 5_4:+13606140 None:intergenic
CTTACAGGTAATTCTCCTTC+TGG 0.343654 5_4:+13607459 None:intergenic
TGAATATCTTGTAGCATTGT+AGG 0.346382 5_4:-13607970 Msa0839000:intron
TCTGAAAATAATCGTTATTC+AGG 0.352379 5_4:-13606983 Msa0839000:CDS
ATATCAAGCAGCAAGGTTTC+AGG 0.359927 5_4:-13606585 Msa0839000:CDS
AGGAACAGGCTACGAGGTTA+AGG 0.366082 5_4:+13608889 None:intergenic
TTGAGGACATGAGAGTCTTT+TGG 0.374336 5_4:-13607882 Msa0839000:five_prime_UTR
AAGCCTTGTGGCGAGGTTGA+TGG 0.376575 5_4:-13606891 Msa0839000:CDS
CTTAGAGTTCTGCTCTTTGA+AGG 0.378221 5_4:+13606446 None:intergenic
AGCCTTGTGGCGAGGTTGAT+GGG 0.378689 5_4:-13606890 Msa0839000:CDS
TCAAGATCAGGAACATCATT+TGG 0.380795 5_4:-13605738 Msa0839000:CDS
ATGGAAGGAGTTCAGTTAGT+TGG 0.381437 5_4:-13606772 Msa0839000:CDS
TGTTCAAAGTTTGCCGAAAT+CGG 0.382797 5_4:-13606560 Msa0839000:CDS
TATTCAATCATGAAGAGAAT+TGG 0.394633 5_4:-13606325 Msa0839000:CDS
GAATCTCTACTGATAAATTC+GGG 0.401716 5_4:+13606008 None:intergenic
GAGAATTGGACTAGTTCAAC+AGG 0.404604 5_4:-13606311 Msa0839000:CDS
TCGGGAACATCCACAGGAAA+TGG 0.408972 5_4:-13606639 Msa0839000:CDS
TGGAAGGAGTTCAGTTAGTT+GGG 0.424198 5_4:-13606771 Msa0839000:CDS
ATCAACCTCGCCACAAGGCT+TGG 0.428948 5_4:+13606893 None:intergenic
GATGTTCCTGATCTTGATAC+AGG 0.455850 5_4:+13605744 None:intergenic
CAACCGCTTCAAGCTTCCTC+CGG 0.459478 5_4:+13605670 None:intergenic
AATGATAATGACAGAGGAAC+AGG 0.459544 5_4:+13608875 None:intergenic
AAGGATCTTACAAGGTTCCT+TGG 0.460932 5_4:+13605774 None:intergenic
AAAAGAATAAGTGAAAATAT+GGG 0.462177 5_4:-13606821 Msa0839000:CDS
TCATGATTGAATATGCAATC+AGG 0.468328 5_4:+13606335 None:intergenic
CTGATAAATTCGGGGACACT+TGG 0.469625 5_4:+13606017 None:intergenic
TTTGAAATGTGCAGATGAAT+GGG 0.480380 5_4:-13605886 Msa0839000:CDS
GAAGAAACAGAAATACAAAC+AGG 0.481676 5_4:-13605852 Msa0839000:CDS
AATAATCGTTATTCAGGCGC+TGG 0.481780 5_4:-13606977 Msa0839000:CDS
TCTGCTCCTGTATCAAGATC+AGG 0.482542 5_4:-13605750 Msa0839000:CDS
GGAACAGGCTACGAGGTTAA+GGG 0.490615 5_4:+13608890 None:intergenic
AATTTATCAGTAGAGATTCA+AGG 0.492213 5_4:-13606004 Msa0839000:CDS
AGTTCAACAGGGACTTTACC+AGG 0.494157 5_4:-13606299 Msa0839000:CDS
CTACAAAGAAAAGTTAGAAC+GGG 0.495839 5_4:-13607940 Msa0839000:five_prime_UTR
CAGGTTTCAAGATAACGATC+CGG 0.509636 5_4:+13606360 None:intergenic
CTGCCATGTGCTTGATCGAT+TGG 0.510292 5_4:+13606922 None:intergenic
CTTCCTCCGGATATGAGCCC+CGG 0.510820 5_4:+13605683 None:intergenic
GCCTTTGTTCTCCAAAACTC+TGG 0.512265 5_4:+13606663 None:intergenic
GCAACAGGTTTAGTGTCTAC+AGG 0.513735 5_4:+13606854 None:intergenic
AGGTTTCAAGATAACGATCC+GGG 0.519687 5_4:+13606361 None:intergenic
GCAATAAGTTTAGGATCAGA+TGG 0.523659 5_4:+13606149 None:intergenic
TTCTGAAGTTCCATTTCCTG+TGG 0.524225 5_4:+13606629 None:intergenic
CGGATCGTTATCTTGAAACC+TGG 0.530582 5_4:-13606359 Msa0839000:CDS
ATGTGGGTGTTGTTGAAAAG+TGG 0.530723 5_4:+13608697 None:intergenic
TGAATTCAATCAAGGGAGGA+TGG 0.531417 5_4:-13607828 Msa0839000:CDS
TTGAATATGCAATCAGGACC+AGG 0.536062 5_4:+13606341 None:intergenic
GAAAGCTTCTACTAGAGGAC+CGG 0.541402 5_4:-13605716 Msa0839000:CDS
ATTATCAAGTAGAAGAAGAC+TGG 0.542682 5_4:-13607006 Msa0839000:CDS
GAATATCTTGTAGCATTGTA+GGG 0.545861 5_4:-13607969 Msa0839000:intron
AGAGAATCGAAACAACGAAA+AGG 0.551559 5_4:-13606510 Msa0839000:CDS
TTTCACAAAGTTAAAGATGA+CGG 0.556814 5_4:-13606704 Msa0839000:CDS
AAGGCGTCGGGAACATCCAC+AGG 0.557978 5_4:-13606645 Msa0839000:CDS
TTCAAGATAACGATCCGGGT+TGG 0.558627 5_4:+13606365 None:intergenic
AAAGATGACGGCGATGATGA+TGG 0.560500 5_4:-13606692 Msa0839000:CDS
TGATTGAGAAAATAGTTATG+TGG 0.562809 5_4:+13608680 None:intergenic
GTGTCTCAATATCACTTCCT+CGG 0.563040 5_4:+13606186 None:intergenic
GCCTTGTGGCGAGGTTGATG+GGG 0.564771 5_4:-13606889 Msa0839000:CDS
ACAGAGAGTTGTTGTCTCCA+AGG 0.575318 5_4:-13605791 Msa0839000:CDS
ACACATGGAAGATGAAATTG+AGG 0.575323 5_4:-13607899 Msa0839000:five_prime_UTR
GAGCCCCGGTTAAAATGTGC+CGG 0.579772 5_4:+13605697 None:intergenic
ACTACAAAGAAAAGTTAGAA+CGG 0.581662 5_4:-13607941 Msa0839000:five_prime_UTR
TCTTCTATACTATGTCTACC+TGG 0.595004 5_4:+13606281 None:intergenic
ATTTGGAAAGCTTCTACTAG+AGG 0.598028 5_4:-13605721 Msa0839000:CDS
GTTGAAACATCTCAGAAGTA+CGG 0.598762 5_4:-13607483 Msa0839000:CDS
AATTCTAGTGGAGTGACACA+TGG 0.599477 5_4:-13607914 Msa0839000:five_prime_UTR
CAAACAAGGGAACAAATGGA+AGG 0.600461 5_4:-13606787 Msa0839000:CDS
ACCCCATCAACCTCGCCACA+AGG 0.600954 5_4:+13606888 None:intergenic
TCTTGATACAGGAGCAGACA+AGG 0.604956 5_4:+13605755 None:intergenic
GATTGAGAAAATAGTTATGT+GGG 0.606247 5_4:+13608681 None:intergenic
TCTCAGAAGTACGGTCCAGA+AGG 0.606677 5_4:-13607474 Msa0839000:CDS
AAAGAATAAGTGAAAATATG+GGG 0.610226 5_4:-13606820 Msa0839000:CDS
TTGAAGCATATCAAGCAGCA+AGG 0.612487 5_4:-13606592 Msa0839000:CDS
GGAAAAGGTTATGCGGTCCG+AGG 0.614051 5_4:-13606203 Msa0839000:CDS
TGAGCAGACTGAATTTGAGT+CGG 0.615214 5_4:+13606082 None:intergenic
CACATAATACAAGTATGCAA+AGG 0.618748 5_4:-13606478 Msa0839000:CDS
ATGACGGCGATGATGATGGA+TGG 0.620775 5_4:-13606688 Msa0839000:CDS
CTGCACCAAGCCTTGTGGCG+AGG 0.620894 5_4:-13606898 Msa0839000:CDS
ACAAGATCATACGAAGGTGA+AGG 0.634923 5_4:-13606053 Msa0839000:CDS
TATCCAATCGATCAAGCACA+TGG 0.637814 5_4:-13606925 Msa0839000:CDS
TAGAACGGGCGTAATTCTAG+TGG 0.640075 5_4:-13607926 Msa0839000:five_prime_UTR
GAGCAGACAAGGATCTTACA+AGG 0.642350 5_4:+13605766 None:intergenic
AACTGTCGGAAAAGGTTATG+CGG 0.647191 5_4:-13606210 Msa0839000:CDS
TAACCGGGGCTCATATCCGG+AGG 0.647535 5_4:-13605686 Msa0839000:CDS
TTGGAGACAACAACTCTCTG+TGG 0.648215 5_4:+13605793 None:intergenic
TAGATGCATCTTCTCCAACC+CGG 0.653096 5_4:-13606379 Msa0839000:CDS
GCTCACAAAAGACATCATAG+TGG 0.659606 5_4:-13607791 Msa0839000:intron
GCAGACTGCACCAAGCCTTG+TGG 0.667683 5_4:-13606903 Msa0839000:CDS
TATCCGGAGGAAGCTTGAAG+CGG 0.676693 5_4:-13605673 Msa0839000:CDS
CGTTTGAAATGCGAACTGCA+AGG 0.679951 5_4:-13606236 Msa0839000:CDS
GTGACTAATGATAATGACAG+AGG 0.684571 5_4:+13608869 None:intergenic
AATCTCTACTGATAAATTCG+GGG 0.689534 5_4:+13606009 None:intergenic
GAATCAACAAGATCATACGA+AGG 0.725807 5_4:-13606059 Msa0839000:CDS
TGACAGAGGAACAGGCTACG+AGG 0.744006 5_4:+13608883 None:intergenic
AGAATTGGACTAGTTCAACA+GGG 0.749710 5_4:-13606310 Msa0839000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607160-13607179 None:intergenic 100.0%
! CGGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607171-13607190 None:intergenic 100.0%
!! TAAAAGAATAAGTGAAAATA+TGG - chr5_4:13607689-13607708 Msa0839000:intron 15.0%
!! AAAAGAATAAGTGAAAATAT+GGG - chr5_4:13607690-13607709 Msa0839000:intron 15.0%
!!! TTCAAAAAGTCTTCTTTTTT+TGG - chr5_4:13605697-13605716 Msa0839000:CDS 20.0%
!!! TCAAAAAGTCTTCTTTTTTT+GGG - chr5_4:13605698-13605717 Msa0839000:CDS 20.0%
!! AAAATCATCATACCAATAAA+TGG + chr5_4:13605906-13605925 None:intergenic 20.0%
!!! ACTTTTTTGAATTCAATCAA+GGG - chr5_4:13606676-13606695 Msa0839000:CDS 20.0%
!! TTCGTATAAAAACAATATGA+TGG + chr5_4:13607114-13607133 None:intergenic 20.0%
!! ATCTTTCTCAATATTCTAAA+AGG + chr5_4:13607257-13607276 None:intergenic 20.0%
!! ACTAAATTCAATTCAACAAA+TGG + chr5_4:13607464-13607483 None:intergenic 20.0%
!! AAAGAATAAGTGAAAATATG+GGG - chr5_4:13607691-13607710 Msa0839000:intron 20.0%
!! GTTATAAGATTTGATCATTA+AGG + chr5_4:13608108-13608127 None:intergenic 20.0%
! CTTTGTGAAAAAATGGAAAT+GGG + chr5_4:13605782-13605801 None:intergenic 25.0%
! ACTTTGTGAAAAAATGGAAA+TGG + chr5_4:13605783-13605802 None:intergenic 25.0%
! CTTCAAACTTTGTGAAAAAA+TGG + chr5_4:13605789-13605808 None:intergenic 25.0%
! GATTGAGAAAATAGTTATGT+GGG + chr5_4:13605833-13605852 None:intergenic 25.0%
! TGATTGAGAAAATAGTTATG+TGG + chr5_4:13605834-13605853 None:intergenic 25.0%
!! TGAATTTTCTCACCATTTAT+TGG - chr5_4:13605891-13605910 Msa0839000:CDS 25.0%
!!! TTTTTTCTTTTGTGTGTGTT+TGG - chr5_4:13605923-13605942 Msa0839000:CDS 25.0%
! TTGTTGTCAAAGTTATGATT+TGG - chr5_4:13606027-13606046 Msa0839000:CDS 25.0%
! TCAACTGAAAACAAGAAATT+GGG + chr5_4:13606191-13606210 None:intergenic 25.0%
! TTCAAAAGCTTACTTGATAT+TGG - chr5_4:13606227-13606246 Msa0839000:CDS 25.0%
!!! AGTTAGTTGTTTCTATTGAT+TGG - chr5_4:13606269-13606288 Msa0839000:CDS 25.0%
!!! TTGTTTTGTATTTGTCCTTA+AGG - chr5_4:13606324-13606343 Msa0839000:CDS 25.0%
! ATGTAAGATTCCTTTATAGA+TGG + chr5_4:13606362-13606381 None:intergenic 25.0%
! ACTACAAAGAAAAGTTAGAA+CGG - chr5_4:13606570-13606589 Msa0839000:CDS 25.0%
!!! CACTTTTTTGAATTCAATCA+AGG - chr5_4:13606675-13606694 Msa0839000:CDS 25.0%
! TACTAAAAACAATCAAGCAA+AGG + chr5_4:13606938-13606957 None:intergenic 25.0%
! CATATCGAACATAATTACAA+CGG + chr5_4:13607232-13607251 None:intergenic 25.0%
! CAAGAACTATGGTTTATATT+TGG + chr5_4:13607418-13607437 None:intergenic 25.0%
! TCTGAAAATAATCGTTATTC+AGG - chr5_4:13607528-13607547 Msa0839000:intron 25.0%
!! TCACTTATTCTTTTATCTGA+TGG + chr5_4:13607684-13607703 None:intergenic 25.0%
! TTTCACAAAGTTAAAGATGA+CGG - chr5_4:13607807-13607826 Msa0839000:CDS 25.0%
! TATTCAATCATGAAGAGAAT+TGG - chr5_4:13608186-13608205 Msa0839000:intron 25.0%
!! TAGTATAGAAGATTTTCTTG+AGG - chr5_4:13608241-13608260 Msa0839000:intron 25.0%
! TGAATCTCTACTGATAAATT+CGG + chr5_4:13608507-13608526 None:intergenic 25.0%
! AATTTATCAGTAGAGATTCA+AGG - chr5_4:13608507-13608526 Msa0839000:intron 25.0%
!! AATGGGTTGAACTAAAAAAG+TGG + chr5_4:13605765-13605784 None:intergenic 30.0%
TGAATGAATCATTCTCTGTT+TGG - chr5_4:13605962-13605981 Msa0839000:CDS 30.0%
TGCTCAAGAAATTTGATGTT+TGG - chr5_4:13606074-13606093 Msa0839000:CDS 30.0%
!!! GAAACTGATTTTTGAGTTGT+TGG - chr5_4:13606121-13606140 Msa0839000:CDS 30.0%
GTCAACTGAAAACAAGAAAT+TGG + chr5_4:13606192-13606211 None:intergenic 30.0%
CTTACTTGATATTGGAAGAT+TGG - chr5_4:13606235-13606254 Msa0839000:CDS 30.0%
AAACTTGAAGAAAGATGTGT+TGG - chr5_4:13606473-13606492 Msa0839000:CDS 30.0%
TGAATATCTTGTAGCATTGT+AGG - chr5_4:13606541-13606560 Msa0839000:CDS 30.0%
GAATATCTTGTAGCATTGTA+GGG - chr5_4:13606542-13606561 Msa0839000:CDS 30.0%
CTACAAAGAAAAGTTAGAAC+GGG - chr5_4:13606571-13606590 Msa0839000:CDS 30.0%
! TTTTTGAATTCAATCAAGGG+AGG - chr5_4:13606679-13606698 Msa0839000:CDS 30.0%
TCCATTCATGTTATGATGAA+AGG - chr5_4:13606798-13606817 Msa0839000:CDS 30.0%
ACCTTTCATCATAACATGAA+TGG + chr5_4:13606802-13606821 None:intergenic 30.0%
AAAAACAATCAAGCAAAGGA+GGG + chr5_4:13606934-13606953 None:intergenic 30.0%
TAAAAACAATCAAGCAAAGG+AGG + chr5_4:13606935-13606954 None:intergenic 30.0%
ACATATGAAGAGAAACTTAC+AGG + chr5_4:13607070-13607089 None:intergenic 30.0%
TGGTGAGAAAATGTACTAAA+AGG + chr5_4:13607327-13607346 None:intergenic 30.0%
! GAAACTGTCATTTATAGAGA+TGG + chr5_4:13607347-13607366 None:intergenic 30.0%
CTCTATAAATGACAGTTTCA+TGG - chr5_4:13607348-13607367 Msa0839000:intron 30.0%
ATAAACCATAGTTCTTGTTC+TGG - chr5_4:13607421-13607440 Msa0839000:intron 30.0%
TAATACCAGAACAAGAACTA+TGG + chr5_4:13607429-13607448 None:intergenic 30.0%
ATTATCAAGTAGAAGAAGAC+TGG - chr5_4:13607505-13607524 Msa0839000:CDS 30.0%
! TTTTCAAACAAGGGAACAAA+TGG - chr5_4:13607720-13607739 Msa0839000:intron 30.0%
GAAATGAGTCGAATTCATTA+TGG + chr5_4:13607791-13607810 None:intergenic 30.0%
CACATAATACAAGTATGCAA+AGG - chr5_4:13608033-13608052 Msa0839000:intron 30.0%
TCATGATTGAATATGCAATC+AGG + chr5_4:13608179-13608198 None:intergenic 30.0%
! TATAGAAGATTTTCTTGAGG+AGG - chr5_4:13608244-13608263 Msa0839000:intron 30.0%
ATTTGACGAGCAATAAGTTT+AGG + chr5_4:13608374-13608393 None:intergenic 30.0%
AATCTCTACTGATAAATTCG+GGG + chr5_4:13608505-13608524 None:intergenic 30.0%
GAATCTCTACTGATAAATTC+GGG + chr5_4:13608506-13608525 None:intergenic 30.0%
!!! TTTTGAAATGTGCAGATGAA+TGG - chr5_4:13608624-13608643 Msa0839000:five_prime_UTR 30.0%
! TTTGAAATGTGCAGATGAAT+GGG - chr5_4:13608625-13608644 Msa0839000:five_prime_UTR 30.0%
GAAGAAACAGAAATACAAAC+AGG - chr5_4:13608659-13608678 Msa0839000:five_prime_UTR 30.0%
AAATACAAACAGGATCTTTC+AGG - chr5_4:13608669-13608688 Msa0839000:five_prime_UTR 30.0%
AGCAAAACTGTATCTCAAAT+TGG + chr5_4:13608933-13608952 None:intergenic 30.0%
! AATGATAATGACAGAGGAAC+AGG + chr5_4:13605639-13605658 None:intergenic 35.0%
! GTGACTAATGATAATGACAG+AGG + chr5_4:13605645-13605664 None:intergenic 35.0%
! GTTTCTAAGAGTGAATTCTG+AGG - chr5_4:13606096-13606115 Msa0839000:CDS 35.0%
! GCAGATTAGTCCATCTATAA+AGG - chr5_4:13606349-13606368 Msa0839000:CDS 35.0%
ACACATGGAAGATGAAATTG+AGG - chr5_4:13606612-13606631 Msa0839000:CDS 35.0%
! AGTCTTTTGGTAGACTGTAT+GGG - chr5_4:13606642-13606661 Msa0839000:CDS 35.0%
! TATGATGTCTTTTGTGAGCA+TGG + chr5_4:13606719-13606738 None:intergenic 35.0%
TGCATATCTTGCTGAAATTC+AGG - chr5_4:13606843-13606862 Msa0839000:CDS 35.0%
!!! GTTTTCCCCCTTATTTTCTT+CGG - chr5_4:13606865-13606884 Msa0839000:CDS 35.0%
TGACAACCGAAGAAAATAAG+GGG + chr5_4:13606874-13606893 None:intergenic 35.0%
GTGACAACCGAAGAAAATAA+GGG + chr5_4:13606875-13606894 None:intergenic 35.0%
TGTGACAACCGAAGAAAATA+AGG + chr5_4:13606876-13606895 None:intergenic 35.0%
GTTGAAACATCTCAGAAGTA+CGG - chr5_4:13607028-13607047 Msa0839000:intron 35.0%
TTCTTATATCCCCTTGTTCT+TGG - chr5_4:13607197-13607216 Msa0839000:intron 35.0%
TCATTGTTACCAAGAACAAG+GGG + chr5_4:13607209-13607228 None:intergenic 35.0%
GTCATTGTTACCAAGAACAA+GGG + chr5_4:13607210-13607229 None:intergenic 35.0%
ATATTGAGAAAGATCCCTCA+AGG - chr5_4:13607263-13607282 Msa0839000:intron 35.0%
! GGGAAGAAGTTTTCAAACAA+GGG - chr5_4:13607711-13607730 Msa0839000:intron 35.0%
TGTTCAAAGTTTGCCGAAAT+CGG - chr5_4:13607951-13607970 Msa0839000:five_prime_UTR 35.0%
AGAGAATCGAAACAACGAAA+AGG - chr5_4:13608001-13608020 Msa0839000:intron 35.0%
!! AGAATTGGACTAGTTCAACA+GGG - chr5_4:13608201-13608220 Msa0839000:intron 35.0%
TCTTCTATACTATGTCTACC+TGG + chr5_4:13608233-13608252 None:intergenic 35.0%
! GCAATAAGTTTAGGATCAGA+TGG + chr5_4:13608365-13608384 None:intergenic 35.0%
GAATCAACAAGATCATACGA+AGG - chr5_4:13608452-13608471 Msa0839000:intron 35.0%
TCAAGATCAGGAACATCATT+TGG - chr5_4:13608773-13608792 Msa0839000:five_prime_UTR 35.0%
ATTTGGAAAGCTTCTACTAG+AGG - chr5_4:13608790-13608809 Msa0839000:five_prime_UTR 35.0%
!! AGATACAGTTTTGCTCTAAG+AGG - chr5_4:13608938-13608957 Msa0839000:five_prime_UTR 35.0%
!!! GATACAGTTTTGCTCTAAGA+GGG - chr5_4:13608939-13608958 Msa0839000:five_prime_UTR 35.0%
!! ATGTGGGTGTTGTTGAAAAG+TGG + chr5_4:13605817-13605836 None:intergenic 40.0%
TGGACTAATCTGCTACCTTA+AGG + chr5_4:13606342-13606361 None:intergenic 40.0%
AATTCTAGTGGAGTGACACA+TGG - chr5_4:13606597-13606616 Msa0839000:CDS 40.0%
! TTGAGGACATGAGAGTCTTT+TGG - chr5_4:13606629-13606648 Msa0839000:CDS 40.0%
! GAGTCTTTTGGTAGACTGTA+TGG - chr5_4:13606641-13606660 Msa0839000:CDS 40.0%
TGAATTCAATCAAGGGAGGA+TGG - chr5_4:13606683-13606702 Msa0839000:CDS 40.0%
TTTGTGAGCATGGACTTTCT+TGG + chr5_4:13606709-13606728 None:intergenic 40.0%
GCTCACAAAAGACATCATAG+TGG - chr5_4:13606720-13606739 Msa0839000:CDS 40.0%
GACAACCGAAGAAAATAAGG+GGG + chr5_4:13606873-13606892 None:intergenic 40.0%
CTTACAGGTAATTCTCCTTC+TGG + chr5_4:13607055-13607074 None:intergenic 40.0%
AGGGGATATAAGAAGTCCTT+CGG + chr5_4:13607191-13607210 None:intergenic 40.0%
GGTCATTGTTACCAAGAACA+AGG + chr5_4:13607211-13607230 None:intergenic 40.0%
AATAATCGTTATTCAGGCGC+TGG - chr5_4:13607534-13607553 Msa0839000:intron 40.0%
TATCCAATCGATCAAGCACA+TGG - chr5_4:13607586-13607605 Msa0839000:intron 40.0%
! CTTTTATCTGATGGAGCAAC+AGG + chr5_4:13607675-13607694 None:intergenic 40.0%
! GGGGAAGAAGTTTTCAAACA+AGG - chr5_4:13607710-13607729 Msa0839000:intron 40.0%
CAAACAAGGGAACAAATGGA+AGG - chr5_4:13607724-13607743 Msa0839000:intron 40.0%
ATGGAAGGAGTTCAGTTAGT+TGG - chr5_4:13607739-13607758 Msa0839000:intron 40.0%
TGGAAGGAGTTCAGTTAGTT+GGG - chr5_4:13607740-13607759 Msa0839000:intron 40.0%
!! TTCTGAAGTTCCATTTCCTG+TGG + chr5_4:13607885-13607904 None:intergenic 40.0%
TTGAAGCATATCAAGCAGCA+AGG - chr5_4:13607919-13607938 Msa0839000:five_prime_UTR 40.0%
ATATCAAGCAGCAAGGTTTC+AGG - chr5_4:13607926-13607945 Msa0839000:five_prime_UTR 40.0%
CTTAGAGTTCTGCTCTTTGA+AGG + chr5_4:13608068-13608087 None:intergenic 40.0%
AGGTTTCAAGATAACGATCC+GGG + chr5_4:13608153-13608172 None:intergenic 40.0%
CAGGTTTCAAGATAACGATC+CGG + chr5_4:13608154-13608173 None:intergenic 40.0%
TTGAATATGCAATCAGGACC+AGG + chr5_4:13608173-13608192 None:intergenic 40.0%
!! GAGAATTGGACTAGTTCAAC+AGG - chr5_4:13608200-13608219 Msa0839000:intron 40.0%
GAACTGCAAGGAAAAACTGT+CGG - chr5_4:13608287-13608306 Msa0839000:intron 40.0%
CAAGGAAAAACTGTCGGAAA+AGG - chr5_4:13608293-13608312 Msa0839000:intron 40.0%
AACTGTCGGAAAAGGTTATG+CGG - chr5_4:13608301-13608320 Msa0839000:intron 40.0%
GTGTCTCAATATCACTTCCT+CGG + chr5_4:13608328-13608347 None:intergenic 40.0%
TGAGCAGACTGAATTTGAGT+CGG + chr5_4:13608432-13608451 None:intergenic 40.0%
ACAAGATCATACGAAGGTGA+AGG - chr5_4:13608458-13608477 Msa0839000:intron 40.0%
AAGGATCTTACAAGGTTCCT+TGG + chr5_4:13608740-13608759 None:intergenic 40.0%
GATGTTCCTGATCTTGATAC+AGG + chr5_4:13608770-13608789 None:intergenic 40.0%
TAGAACGGGCGTAATTCTAG+TGG - chr5_4:13606585-13606604 Msa0839000:CDS 45.0%
! CTTTTGGTAGACTGTATGGG+AGG - chr5_4:13606645-13606664 Msa0839000:CDS 45.0%
CAATCAAGCAAAGGAGGGAA+GGG + chr5_4:13606929-13606948 None:intergenic 45.0%
ACAATCAAGCAAAGGAGGGA+AGG + chr5_4:13606930-13606949 None:intergenic 45.0%
GATGAGTTTCAAAGTCTGCG+TGG + chr5_4:13606988-13607007 None:intergenic 45.0%
ATATAAGAAGTCCTTCGGGG+GGG + chr5_4:13607186-13607205 None:intergenic 45.0%
GATATAAGAAGTCCTTCGGG+GGG + chr5_4:13607187-13607206 None:intergenic 45.0%
GGATATAAGAAGTCCTTCGG+GGG + chr5_4:13607188-13607207 None:intergenic 45.0%
GGGATATAAGAAGTCCTTCG+GGG + chr5_4:13607189-13607208 None:intergenic 45.0%
GGGGATATAAGAAGTCCTTC+GGG + chr5_4:13607190-13607209 None:intergenic 45.0%
AGAAAGATCCCTCAAGGTTG+TGG - chr5_4:13607269-13607288 Msa0839000:intron 45.0%
GAAAGATCCCTCAAGGTTGT+GGG - chr5_4:13607270-13607289 Msa0839000:intron 45.0%
GGAGAGTAGAGCACATGTAT+AGG - chr5_4:13607291-13607310 Msa0839000:intron 45.0%
GTAGAGCACATGTATAGGCT+TGG - chr5_4:13607296-13607315 Msa0839000:intron 45.0%
GCAACAGGTTTAGTGTCTAC+AGG + chr5_4:13607660-13607679 None:intergenic 45.0%
! AAAGATGACGGCGATGATGA+TGG - chr5_4:13607819-13607838 Msa0839000:CDS 45.0%
!!! GCCAGAGTTTTGGAGAACAA+AGG - chr5_4:13607847-13607866 Msa0839000:CDS 45.0%
GCCTTTGTTCTCCAAAACTC+TGG + chr5_4:13607851-13607870 None:intergenic 45.0%
!!! GTTTTGGAGAACAAAGGCGT+CGG - chr5_4:13607853-13607872 Msa0839000:CDS 45.0%
!!! TTTTGGAGAACAAAGGCGTC+GGG - chr5_4:13607854-13607873 Msa0839000:CDS 45.0%
!! GCTAGAAACACTGCCGATTT+CGG + chr5_4:13607967-13607986 None:intergenic 45.0%
TAGATGCATCTTCTCCAACC+CGG - chr5_4:13608132-13608151 Msa0839000:intron 45.0%
TTCAAGATAACGATCCGGGT+TGG + chr5_4:13608149-13608168 None:intergenic 45.0%
CGGATCGTTATCTTGAAACC+TGG - chr5_4:13608152-13608171 Msa0839000:intron 45.0%
AGTTCAACAGGGACTTTACC+AGG - chr5_4:13608212-13608231 Msa0839000:intron 45.0%
! CGTTTGAAATGCGAACTGCA+AGG - chr5_4:13608275-13608294 Msa0839000:intron 45.0%
CTGATAAATTCGGGGACACT+TGG + chr5_4:13608497-13608516 None:intergenic 45.0%
TTGGAGACAACAACTCTCTG+TGG + chr5_4:13608721-13608740 None:intergenic 45.0%
ACAGAGAGTTGTTGTCTCCA+AGG - chr5_4:13608720-13608739 Msa0839000:five_prime_UTR 45.0%
GAGCAGACAAGGATCTTACA+AGG + chr5_4:13608748-13608767 None:intergenic 45.0%
TCTTGATACAGGAGCAGACA+AGG + chr5_4:13608759-13608778 None:intergenic 45.0%
TCTGCTCCTGTATCAAGATC+AGG - chr5_4:13608761-13608780 Msa0839000:five_prime_UTR 45.0%
GAAAGCTTCTACTAGAGGAC+CGG - chr5_4:13608795-13608814 Msa0839000:five_prime_UTR 45.0%
! TTTTAACCGGGGCTCATATC+CGG - chr5_4:13608822-13608841 Msa0839000:five_prime_UTR 45.0%
!!! ACAGTTTTGCTCTAAGAGGG+AGG - chr5_4:13608942-13608961 Msa0839000:five_prime_UTR 45.0%
GGAACAGGCTACGAGGTTAA+GGG + chr5_4:13605624-13605643 None:intergenic 50.0%
AGGAACAGGCTACGAGGTTA+AGG + chr5_4:13605625-13605644 None:intergenic 50.0%
! TCTCAGAAGTACGGTCCAGA+AGG - chr5_4:13607037-13607056 Msa0839000:intron 50.0%
TATAAGAAGTCCTTCGGGGG+GGG + chr5_4:13607185-13607204 None:intergenic 50.0%
CTACTCTCCCACAACCTTGA+GGG + chr5_4:13607280-13607299 None:intergenic 50.0%
TCTACTCTCCCACAACCTTG+AGG + chr5_4:13607281-13607300 None:intergenic 50.0%
CTGCCATGTGCTTGATCGAT+TGG + chr5_4:13607592-13607611 None:intergenic 50.0%
!! ATGACGGCGATGATGATGGA+TGG - chr5_4:13607823-13607842 Msa0839000:CDS 50.0%
! GATGGATGGAGCCAGAGTTT+TGG - chr5_4:13607837-13607856 Msa0839000:CDS 50.0%
TCGGGAACATCCACAGGAAA+TGG - chr5_4:13607872-13607891 Msa0839000:exon 50.0%
!! GAGGACCGGCACATTTTAAC+CGG - chr5_4:13608809-13608828 Msa0839000:five_prime_UTR 50.0%
!! AGGACCGGCACATTTTAACC+GGG - chr5_4:13608810-13608829 Msa0839000:five_prime_UTR 50.0%
TATCCGGAGGAAGCTTGAAG+CGG - chr5_4:13608838-13608857 Msa0839000:five_prime_UTR 50.0%
TGACAGAGGAACAGGCTACG+AGG + chr5_4:13605631-13605650 None:intergenic 55.0%
GCAAAGGAGGGAAGGGAACA+GGG + chr5_4:13606922-13606941 None:intergenic 55.0%
AGCAAAGGAGGGAAGGGAAC+AGG + chr5_4:13606923-13606942 None:intergenic 55.0%
ATAAGAAGTCCTTCGGGGGG+GGG + chr5_4:13607184-13607203 None:intergenic 55.0%
ATCAACCTCGCCACAAGGCT+TGG + chr5_4:13607621-13607640 None:intergenic 55.0%
! AAGCCTTGTGGCGAGGTTGA+TGG - chr5_4:13607620-13607639 Msa0839000:intron 55.0%
! AGCCTTGTGGCGAGGTTGAT+GGG - chr5_4:13607621-13607640 Msa0839000:intron 55.0%
GGAAAAGGTTATGCGGTCCG+AGG - chr5_4:13608308-13608327 Msa0839000:intron 55.0%
!! GGACCGGCACATTTTAACCG+GGG - chr5_4:13608811-13608830 Msa0839000:five_prime_UTR 55.0%
GAGCCCCGGTTAAAATGTGC+CGG + chr5_4:13608817-13608836 None:intergenic 55.0%
CAACCGCTTCAAGCTTCCTC+CGG + chr5_4:13608844-13608863 None:intergenic 55.0%
TAAGAAGTCCTTCGGGGGGG+GGG + chr5_4:13607183-13607202 None:intergenic 60.0%
GCAGACTGCACCAAGCCTTG+TGG - chr5_4:13607608-13607627 Msa0839000:intron 60.0%
!! GCCTTGTGGCGAGGTTGATG+GGG - chr5_4:13607622-13607641 Msa0839000:intron 60.0%
ACCCCATCAACCTCGCCACA+AGG + chr5_4:13607626-13607645 None:intergenic 60.0%
!! AAGGCGTCGGGAACATCCAC+AGG - chr5_4:13607866-13607885 Msa0839000:exon 60.0%
TAACCGGGGCTCATATCCGG+AGG - chr5_4:13608825-13608844 Msa0839000:five_prime_UTR 60.0%
CTTCCTCCGGATATGAGCCC+CGG + chr5_4:13608831-13608850 None:intergenic 60.0%
AAGAAGTCCTTCGGGGGGGG+GGG + chr5_4:13607182-13607201 None:intergenic 65.0%
! CTGCACCAAGCCTTGTGGCG+AGG - chr5_4:13607613-13607632 Msa0839000:intron 65.0%
GGGGGGGGGGATTCGAACTC+AGG + chr5_4:13607150-13607169 None:intergenic 70.0%
AGAAGTCCTTCGGGGGGGGG+GGG + chr5_4:13607181-13607200 None:intergenic 70.0%
AAGTCCTTCGGGGGGGGGGG+GGG + chr5_4:13607179-13607198 None:intergenic 75.0%
GAAGTCCTTCGGGGGGGGGG+GGG + chr5_4:13607180-13607199 None:intergenic 75.0%
! AGTCCTTCGGGGGGGGGGGG+GGG + chr5_4:13607178-13607197 None:intergenic 80.0%
! TCCTTCGGGGGGGGGGGGGG+GGG + chr5_4:13607176-13607195 None:intergenic 85.0%
! GTCCTTCGGGGGGGGGGGGG+GGG + chr5_4:13607177-13607196 None:intergenic 85.0%
! TTCGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607173-13607192 None:intergenic 90.0%
! CTTCGGGGGGGGGGGGGGGG+GGG + chr5_4:13607174-13607193 None:intergenic 90.0%
! CCTTCGGGGGGGGGGGGGGG+GGG + chr5_4:13607175-13607194 None:intergenic 90.0%
! TCGGGGGGGGGGGGGGGGGG+GGG + chr5_4:13607172-13607191 None:intergenic 95.0%
! CCCCCCCCCCCCCCCCCCGA+AGG - chr5_4:13607172-13607191 Msa0839000:intron 95.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 13605563 13608970 13605563 ID=Msa0839000;Name=Msa0839000
chr5_4 mRNA 13605563 13608970 13605563 ID=Msa0839000-mRNA-1;Parent=Msa0839000;Name=Msa0839000-mRNA-1;_AED=0.02;_eAED=0.02;_QI=455|1|0.75|1|0.66|0.25|4|0|543
chr5_4 exon 13605563 13607032 13605563 ID=Msa0839000-mRNA-1:exon:6092;Parent=Msa0839000-mRNA-1
chr5_4 exon 13607464 13607540 13607464 ID=Msa0839000-mRNA-1:exon:6091;Parent=Msa0839000-mRNA-1
chr5_4 exon 13607792 13607978 13607792 ID=Msa0839000-mRNA-1:exon:6090;Parent=Msa0839000-mRNA-1
chr5_4 exon 13608621 13608970 13608621 ID=Msa0839000-mRNA-1:exon:6089;Parent=Msa0839000-mRNA-1
chr5_4 five_prime_UTR 13608621 13608970 13608621 ID=Msa0839000-mRNA-1:five_prime_utr;Parent=Msa0839000-mRNA-1
chr5_4 five_prime_UTR 13607874 13607978 13607874 ID=Msa0839000-mRNA-1:five_prime_utr;Parent=Msa0839000-mRNA-1
chr5_4 CDS 13607792 13607873 13607792 ID=Msa0839000-mRNA-1:cds;Parent=Msa0839000-mRNA-1
chr5_4 CDS 13607464 13607540 13607464 ID=Msa0839000-mRNA-1:cds;Parent=Msa0839000-mRNA-1
chr5_4 CDS 13605563 13607032 13605563 ID=Msa0839000-mRNA-1:cds;Parent=Msa0839000-mRNA-1
Gene Sequence

>Msa0839000

ATGGGAGGTTTATTGCACTTTTTTGAATTCAATCAAGGGAGGATGGCCAAGAAAGTCCATGCTCACAAAAGACATCATAGTGACTTTGAAACTCATCGAAATAGTCTAGATCTGCAAGTTGAAACATCTCAGAAGTACGGTCCAGAAGGAGAATTACCTCACTATTATCAAGTAGAAGAAGACTGGTCTGAAAATAATCGTTATTCAGGCGCTGGTTCAATGAAAAAGCTGATCAATGAAGAACTATCCAATCGATCAAGCACATGGCAGACTGCACCAAGCCTTGTGGCGAGGTTGATGGGGTTAGATATGATGCCTGTAGACACTAAACCTGTTGCTCCATCAGATAAAAGAATAAGTGAAAATATGGGGAAGAAGTTTTCAAACAAGGGAACAAATGGAAGGAGTTCAGTTAGTTGGGAATCTTCTAACTTTAATTCTTCTAGCCATAATGAATTCGACTCATTTCACAAAGTTAAAGATGACGGCGATGATGATGGATGGAGCCAGAGTTTTGGAGAACAAAGGCGTCGGGAACATCCACAGGAAATGGAACTTCAGAAATTCAAGAAAGAGTTTGAAGCATATCAAGCAGCAAGGTTTCAGGAATGTTCAAAGTTTGCCGAAATCGGCAGTGTTTCTAGCAGATTGATTTTTCAAGAGAATCGAAACAACGAAAAGGTTGCACTTGCACATAATACAAGTATGCAAAGGAAAATATTTCCTTCAAAGAGCAGAACTCTAAGTAGAGACTTTGAAGAGTCCTTAATGATCAAATCTTATAACAGATTAGATGCATCTTCTCCAACCCGGATCGTTATCTTGAAACCTGGTCCTGATTGCATATTCAATCATGAAGAGAATTGGACTAGTTCAACAGGGACTTTACCAGGTAGACATAGTATAGAAGATTTTCTTGAGGAGGTGAAAGAGCGTTTGAAATGCGAACTGCAAGGAAAAACTGTCGGAAAAGGTTATGCGGTCCGAGGAAGTGATATTGAGACACTATGCAATAAAAAACCATCTGATCCTAAACTTATTGCTCGTCAAATAGTAAAGCAAGTCAAAGAAAATGTGACTAGAGATACCGACTCAAATTCAGTCTGCTCAGAATCAACAAGATCATACGAAGGTGAAGGACAATTCAATGAACCAAGTGTCCCCGAATTTATCAGTAGAGATTCAAGGAGTTACTTGTCAGAGAAGCTAACAGATGTTGTAAAAAGTGAAAGACATGATACATTTGACAATCACAGATTTAGACTCAAGAAGAGAGAAGATATTTTGAAATGTGCAGATGAATGGGAAATATCGAAAGAAGAAACAGAAATACAAACAGGATCTTTCAGGCATGAACAAGACAACAACATATTGTTCCACAGAGAGTTGTTGTCTCCAAGGAACCTTGTAAGATCCTTGTCTGCTCCTGTATCAAGATCAGGAACATCATTTGGAAAGCTTCTACTAGAGGACCGGCACATTTTAACCGGGGCTCATATCCGGAGGAAGCTTGAAGCGGTTGAAACTATGTCTCTTGATGTTAAAAAACAGAAAAAAGAGAGATTTAATAATATTAAAGAAAAAGTTTCCAATTTGAGATACAGTTTTGCTCTAAGAGGGAGGATTTTT
Protein sequence

>Msa0839000

MGGLLHFFEFNQGRMAKKVHAHKRHHSDFETHRNSLDLQVETSQKYGPEGELPHYYQVEEDWSENNRYSGAGSMKKLINEELSNRSSTWQTAPSLVARLMGLDMMPVDTKPVAPSDKRISENMGKKFSNKGTNGRSSVSWESSNFNSSSHNEFDSFHKVKDDGDDDGWSQSFGEQRRREHPQEMELQKFKKEFEAYQAARFQECSKFAEIGSVSSRLIFQENRNNEKVALAHNTSMQRKIFPSKSRTLSRDFEESLMIKSYNRLDASSPTRIVILKPGPDCIFNHEENWTSSTGTLPGRHSIEDFLEEVKERLKCELQGKTVGKGYAVRGSDIETLCNKKPSDPKLIARQIVKQVKENVTRDTDSNSVCSESTRSYEGEGQFNEPSVPEFISRDSRSYLSEKLTDVVKSERHDTFDNHRFRLKKREDILKCADEWEISKEETEIQTGSFRHEQDNNILFHRELLSPRNLVRSLSAPVSRSGTSFGKLLLEDRHILTGAHIRRKLEAVETMSLDVKKQKKERFNNIKEKVSNLRYSFALRGRIF