AlfalfaGEDB | Msa0747650

M. sativa cultivar zhongmu-4 / Msa0747650

KEGG: -
GO: GO:0001101 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009892 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010267 GO:0010305 GO:0010445 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031054 GO:0031974 GO:0031981 GO:0032296 GO:0032501 GO:0032502 GO:0033993 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0035198 GO:0035279 GO:0040029 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097305 GO:0098795 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700
PFAM: PF00035.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0747650	sp\|O04492\|DRB1_ARATH	64.362	188	66	1	12	199	9	195	3.46e-77	255

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0747650	G7J4K9	96.828	662	21	0	1	662	1	662	0.0	1310

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006510	Msa0747650	0.837800	4.198276e-57	-8.615850e-47
Msa0015670	Msa0747650	0.835185	1.944027e-56	-8.615850e-47
Msa0042550	Msa0747650	0.808141	3.582431e-50	-8.615850e-47
Msa0045550	Msa0747650	0.821401	4.116753e-53	-8.615850e-47
Msa0045910	Msa0747650	0.823439	1.384166e-53	-8.615850e-47
Msa0055070	Msa0747650	0.823365	1.440643e-53	-8.615850e-47
Msa0077450	Msa0747650	0.818600	1.800793e-52	-8.615850e-47
Msa0089870	Msa0747650	0.800172	1.632541e-48	-8.615850e-47
Msa0101020	Msa0747650	0.815211	1.038260e-51	-8.615850e-47
Msa0119320	Msa0747650	0.809733	1.635612e-50	-8.615850e-47
Msa0624120	Msa0747650	0.800261	1.565564e-48	-8.615850e-47
Msa0689760	Msa0747650	0.813948	1.976500e-51	-8.615850e-47
Msa0718070	Msa0747650	0.813889	2.036743e-51	-8.615850e-47
Msa0747650	Msa0750250	0.805881	1.077465e-49	-8.615850e-47
Msa0747650	Msa0751260	0.820028	8.517093e-53	-8.615850e-47
Msa0747650	Msa0780280	0.812260	4.635740e-51	-8.615850e-47
Msa0747650	Msa0800770	0.811830	5.754312e-51	-8.615850e-47
Msa0747650	Msa0809140	0.802112	6.548010e-49	-8.615850e-47
Msa0747650	Msa0821800	0.805694	1.179875e-49	-8.615850e-47
Msa0747650	Msa0827150	0.832162	1.106182e-55	-8.615850e-47
Msa0747650	Msa0838930	0.823175	1.595705e-53	-8.615850e-47
Msa0747650	Msa0839000	0.810095	1.366430e-50	-8.615850e-47
Msa0747650	Msa0902710	0.802775	4.779617e-49	-8.615850e-47
Msa0747650	Msa0939740	0.803282	3.755901e-49	-8.615850e-47
Msa0747650	Msa0981310	0.803229	3.851811e-49	-8.615850e-47
Msa0747650	Msa1000190	0.805104	1.567960e-49	-8.615850e-47
Msa0747650	Msa1008750	0.838237	3.240840e-57	-8.615850e-47
Msa0747650	Msa1009390	0.819628	1.050858e-52	-8.615850e-47
Msa0747650	Msa1014010	0.800258	1.568338e-48	-8.615850e-47
Msa0747650	Msa1070840	0.809952	1.467350e-50	-8.615850e-47
Msa0747650	Msa1093480	0.822739	2.016217e-53	-8.615850e-47
Msa0747650	Msa1096060	0.845316	4.381977e-59	-8.615850e-47
Msa0747650	Msa1098270	0.837910	3.932357e-57	-8.615850e-47
Msa0747650	Msa1098290	0.826730	2.310565e-54	-8.615850e-47
Msa0747650	Msa1118760	0.839543	1.488359e-57	-8.615850e-47
Msa0747650	Msa1133570	0.810115	1.352911e-50	-8.615850e-47
Msa0747650	Msa1138280	0.812962	3.255208e-51	-8.615850e-47
Msa0747650	Msa1138320	0.843785	1.132015e-58	-8.615850e-47
Msa0747650	Msa1158660	0.833302	5.765832e-56	-8.615850e-47
Msa0747650	Msa1170130	0.808981	2.371253e-50	-8.615850e-47
Msa0747650	Msa1225200	0.841685	4.090278e-58	-8.615850e-47
Msa0747650	Msa1288450	0.801317	9.533172e-49	-8.615850e-47
Msa0747650	Msa1319820	0.801517	8.672874e-49	-8.615850e-47
Msa0747650	Msa1359920	0.864014	1.648846e-64	-8.615850e-47
Msa0747650	Msa1366300	0.815845	7.503855e-52	-8.615850e-47
Msa0747650	Msa1372150	0.808184	3.507717e-50	-8.615850e-47
Msa0747650	Msa1379850	0.831822	1.342255e-55	-8.615850e-47
Msa0747650	Msa1400070	0.814885	1.226616e-51	-8.615850e-47
Msa0747650	Msa1420250	0.812254	4.650434e-51	-8.615850e-47
Msa0747650	Msa1426570	0.803095	4.104763e-49	-8.615850e-47
Msa0747650	Msa1433220	0.835506	1.612767e-56	-8.615850e-47
Msa0747650	Msa1445220	0.817375	3.407140e-52	-8.615850e-47
Msa0747650	Msa1446780	0.809359	1.967947e-50	-8.615850e-47
Msa0747650	Msa1459050	0.802826	4.666496e-49	-8.615850e-47
Msa0145110	Msa0747650	0.802571	5.266124e-49	-8.615850e-47
Msa0168630	Msa0747650	0.819582	1.077058e-52	-8.615850e-47
Msa0169440	Msa0747650	0.819962	8.817285e-53	-8.615850e-47
Msa0170140	Msa0747650	0.801774	7.682701e-49	-8.615850e-47
Msa0178510	Msa0747650	0.806338	8.634396e-50	-8.615850e-47
Msa0187030	Msa0747650	0.806636	7.472949e-50	-8.615850e-47
Msa0193540	Msa0747650	0.845604	3.660548e-59	-8.615850e-47
Msa0200560	Msa0747650	0.814267	1.681022e-51	-8.615850e-47
Msa0225640	Msa0747650	0.820791	5.691731e-53	-8.615850e-47
Msa0227230	Msa0747650	0.813233	2.839786e-51	-8.615850e-47
Msa0235470	Msa0747650	0.814782	1.293163e-51	-8.615850e-47
Msa0377440	Msa0747650	0.819388	1.192548e-52	-8.615850e-47
Msa0379710	Msa0747650	0.995832	2.200636e-220	-8.615850e-47
Msa0390960	Msa0747650	0.800350	1.502189e-48	-8.615850e-47
Msa0429090	Msa0747650	0.920183	1.765896e-87	-8.615850e-47
Msa0436370	Msa0747650	0.832637	8.437427e-56	-8.615850e-47
Msa0475500	Msa0747650	0.930828	9.264528e-94	-8.615850e-47
Msa0243250	Msa0747650	0.836287	1.022306e-56	-8.615850e-47
Msa0274630	Msa0747650	0.849545	3.018387e-60	-8.615850e-47
Msa0276750	Msa0747650	-0.800285	1.548106e-48	-8.615850e-47
Msa0277420	Msa0747650	0.809569	1.773637e-50	-8.615850e-47
Msa0314060	Msa0747650	0.827467	1.539595e-54	-8.615850e-47
Msa0317270	Msa0747650	0.822884	1.865564e-53	-8.615850e-47
Msa0339280	Msa0747650	0.825514	4.498229e-54	-8.615850e-47
Msa0517650	Msa0747650	0.809391	1.936368e-50	-8.615850e-47
Msa0526100	Msa0747650	-0.802845	4.624761e-49	-8.615850e-47
Msa0572420	Msa0747650	0.840374	9.037275e-58	-8.615850e-47
Msa0572480	Msa0747650	0.828762	7.508337e-55	-8.615850e-47
Msa0573040	Msa0747650	0.817364	3.427218e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0747650	MtrunA17_Chr3g0132821	96.526	662	21	1	1	662	1	660	0.0	1300
Msa0747650	MtrunA17_Chr7g0273611	38.462	156	94	1	18	173	1	154	3.84e-25	109
Msa0747650	MtrunA17_Chr4g0029001	37.500	152	93	1	18	169	1	150	1.15e-21	98.6
Msa0747650	MtrunA17_Chr2g0326721	36.842	152	94	1	18	169	1	150	1.66e-21	97.4
Msa0747650	MtrunA17_Chr4g0002041	34.177	158	100	2	19	176	28	181	1.19e-20	93.6
Msa0747650	MtrunA17_Chr5g0435011	33.333	159	102	2	18	176	27	181	2.57e-19	91.3
Msa0747650	MtrunA17_Chr6g0461131	35.625	160	89	4	17	173	157	305	3.50e-17	85.1
Msa0747650	MtrunA17_Chr3g0118921	32.468	154	93	4	20	173	50	192	1.82e-13	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0747650	AT1G09700.2	64.362	188	66	1	12	199	9	195	8.53e-80	255
Msa0747650	AT1G09700.1	64.362	188	66	1	12	199	9	195	3.52e-78	255
Msa0747650	AT3G26932.1	40.385	156	91	1	18	173	1	154	5.63e-28	116
Msa0747650	AT3G26932.2	40.385	156	91	1	18	173	1	154	5.63e-28	116
Msa0747650	AT5G41070.1	32.243	214	125	3	18	231	1	194	5.27e-24	105
Msa0747650	AT2G28380.1	36.196	163	102	1	18	180	1	161	3.10e-22	100
Msa0747650	AT3G62800.3	31.839	223	135	5	17	239	3	208	1.06e-21	97.1
Msa0747650	AT3G62800.2	31.839	223	135	5	17	239	3	208	1.52e-21	97.4
Msa0747650	AT3G62800.1	31.839	223	135	5	17	239	3	208	1.52e-21	97.4

Find 148 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATCGTATATTATTTATTT+TGG	0.107367	5_2:+430144	None:intergenic
CTTCCCATGAACCTTCTTTC+AGG	0.162473	5_2:-428794	Msa0747650:CDS
ATCTAAATTAAGAATATTTC+TGG	0.176270	5_2:+430199	None:intergenic
TCTAAGAAATCACGCTTTAA+AGG	0.215863	5_2:-427674	Msa0747650:CDS
CTGTCATTCCATCCCGAAAA+AGG	0.224505	5_2:-427739	Msa0747650:CDS
ATTTCTATGTGGTTTCTTCT+TGG	0.224812	5_2:+427643	None:intergenic
ATTCCCAATATTGATCTCTT+TGG	0.236860	5_2:+426437	None:intergenic
TCAGAAGTCATGAAGAATTC+TGG	0.244468	5_2:-426561	Msa0747650:CDS
CTGCTTCAGATTTGATTTCC+TGG	0.251417	5_2:+426592	None:intergenic
CTGCTTCAGATTTGATTTCC+TGG	0.251417	5_2:+426799	None:intergenic
CTGCTTCAGATTTGATTTCC+TGG	0.251417	5_2:+426904	None:intergenic
CTGCTTCAGATTTGATTTCC+TGG	0.267375	5_2:+426694	None:intergenic
CTGCTTCAGATTTGATTTCC+TGG	0.275383	5_2:+427423	None:intergenic
CTGCTTCAGATTTGATTTCC+TGG	0.275383	5_2:+427525	None:intergenic
CAATCCAAAGAGATCAATAT+TGG	0.281542	5_2:-426441	Msa0747650:CDS
CTGCTTCAGATTTGACTTCC+TGG	0.293244	5_2:+427009	None:intergenic
AGCTGCAGAGGTTGCTTTGA+TGG	0.313250	5_2:-428697	Msa0747650:CDS
CTGCTCCAGATTTGACTTCC+TGG	0.313787	5_2:+427321	None:intergenic
CTGCTTCAGATTTAACTTCC+TGG	0.322914	5_2:+427219	None:intergenic
GATGGAGCAAAGATCAATAA+TGG	0.336169	5_2:-427587	Msa0747650:CDS
TCCGTTCTCAGAATTCTTCA+TGG	0.354796	5_2:+427283	None:intergenic
TGCTCCCGAGGGATACATAT+TGG	0.360088	5_2:-426394	Msa0747650:CDS
GCCATGCAGTATTATGATAA+TGG	0.370303	5_2:-427386	Msa0747650:CDS
ATTGGAGGGATGCTCTATAT+TGG	0.371085	5_2:-427872	Msa0747650:CDS
AGAGACATTAGCTGCAATAA+AGG	0.374142	5_2:-427132	Msa0747650:CDS
GAATTCTGCATGGCTTCATC+TGG	0.378975	5_2:+427188	None:intergenic
ATTGTGCAAGAATTTACTTC+AGG	0.382437	5_2:-427993	Msa0747650:CDS
AAGCGTGATTTCTTAGACTT+CGG	0.393072	5_2:+427680	None:intergenic
GGCCTCCAGGAAGTCAAATC+TGG	0.396478	5_2:-427326	Msa0747650:CDS
TCTGTGCATACTCTTGCAAT+TGG	0.398490	5_2:+428862	None:intergenic
GCCATTGTATCAGAGCAAAA+AGG	0.401184	5_2:-427942	Msa0747650:CDS
TGACAGTCAAATGGCCAAAC+TGG	0.404308	5_2:+427756	None:intergenic
TTGATGGAGTTGGCTAAAAC+TGG	0.412242	5_2:-428681	Msa0747650:CDS
CGATGCAGAATTCTGAGAAC+GGG	0.413538	5_2:-426863	Msa0747650:CDS
CAGGCTTCTGAAAACCAGTT+TGG	0.414886	5_2:-427770	Msa0747650:CDS
TGCAATAAAGGAAGTCAAAT+CGG	0.421774	5_2:-427120	Msa0747650:CDS
CTAAGAAATCACGCTTTAAA+GGG	0.422488	5_2:-427673	Msa0747650:CDS
CCATGCAGAATTCTGAGAAC+GGG	0.423049	5_2:-426968	Msa0747650:CDS
CAGAAGTCATGAAGAATTCT+GGG	0.423704	5_2:-426560	Msa0747650:CDS
AGATGAAATTGAAGAGTGGT+TGG	0.426963	5_2:+429967	None:intergenic
TCAACCGTACACGAATATAA+TGG	0.434098	5_2:+427896	None:intergenic
TTGACCTGAAAGAAGGTTCA+TGG	0.434596	5_2:+428790	None:intergenic
AGAGGTTGCTTTGATGGAGT+TGG	0.436246	5_2:-428691	Msa0747650:CDS
ACAGAATTGCAATGGAGATA+TGG	0.444900	5_2:-426349	Msa0747650:CDS
ATGGAGCAAAGATCAATAAT+GGG	0.448036	5_2:-427586	Msa0747650:CDS
ACCTTGGAAATCTTCGTTAG+TGG	0.448531	5_2:+429919	None:intergenic
GTTCGGTATGATTCTTTGAC+TGG	0.460775	5_2:-428750	Msa0747650:CDS
AATCCAAAGAGATCAATATT+GGG	0.463678	5_2:-426440	Msa0747650:CDS
CAATAATGGGGAAGAATCAC+TGG	0.468073	5_2:-427573	Msa0747650:CDS
AGAGACATTAGCTGCAGTCC+AGG	0.469629	5_2:-426712	Msa0747650:CDS
CAGCATTGTCAGTGTTAATA+CGG	0.476206	5_2:+427612	None:intergenic
AAGCCTGGGATGCATTTGTC+TGG	0.480978	5_2:+427786	None:intergenic
TGCAATGGAGATATGGTCTC+TGG	0.488580	5_2:-426342	Msa0747650:CDS
AGCAGAGCAGTCAGCTGCAG+AGG	0.490356	5_2:-428709	Msa0747650:CDS
TTCTGCATGGCTTCATCTGA+AGG	0.494295	5_2:+426981	None:intergenic
TTCTGCATGGCTTCATCTGA+AGG	0.494295	5_2:+427086	None:intergenic
GCAAGTGCTAATGATGAATC+TGG	0.496570	5_2:-427551	Msa0747650:CDS
TTCAGATTTGATTTCCTGGA+TGG	0.500082	5_2:+426803	None:intergenic
GCCATGACAAATTCTGAAAA+CGG	0.500363	5_2:-426759	Msa0747650:CDS
TTCAGATTTGATTTCCTGGA+TGG	0.500978	5_2:+426908	None:intergenic
TAATGATGAATCTGGCCTCC+AGG	0.501653	5_2:-427543	Msa0747650:CDS
CGTATTAACACTGACAATGC+TGG	0.505782	5_2:-427611	Msa0747650:CDS
AGAGACATTAGCTGGCCTCC+AGG	0.509998	5_2:-427237	Msa0747650:CDS
AGAGACATTAGCTGGCCTCC+AGG	0.509998	5_2:-427339	Msa0747650:CDS
TTCTGCATCGCTTCATCTGA+AGG	0.512503	5_2:+426876	None:intergenic
CATGAGAAAGAGACATTAGC+TGG	0.514047	5_2:-426618	Msa0747650:CDS
AGAAACATTAGCTGCCATCC+AGG	0.514509	5_2:-427027	Msa0747650:CDS
GATGAAATTGAAGAGTGGTT+GGG	0.515775	5_2:+429968	None:intergenic
CGGCGCTTCTTATTTCTATG+TGG	0.516238	5_2:+427632	None:intergenic
ATCTGAAGCAGCATTCCCTT+CGG	0.519137	5_2:-427411	Msa0747650:CDS
CATGAGAAAGAGACATTAGC+TGG	0.520527	5_2:-427245	Msa0747650:CDS
AGAGACATTAGCTGCCATCC+AGG	0.520770	5_2:-426817	Msa0747650:CDS
GAATATAATGGTGCCCTACC+TGG	0.523233	5_2:+427908	None:intergenic
ATCACTGTTGACCTGAAAGA+AGG	0.527088	5_2:+428783	None:intergenic
TTCAGATTTGACTTCCTGGA+TGG	0.527890	5_2:+427013	None:intergenic
TCCTTAGTAGTTTCATACAC+AGG	0.528972	5_2:+428822	None:intergenic
CAGTGATTATGAATGATGTT+CGG	0.529179	5_2:-428767	Msa0747650:CDS
TATTCGTGTACGGTTGACAT+TGG	0.530174	5_2:-427890	Msa0747650:CDS
ACCTGTGTATGAAACTACTA+AGG	0.531516	5_2:-428823	Msa0747650:CDS
AGAGACATTAGCTGGCCTCC+AGG	0.532686	5_2:-427441	Msa0747650:CDS
CATGAGAAAGAGACATTAGC+TGG	0.534007	5_2:-427449	Msa0747650:CDS
CATGAGAAAGAGACATTAGC+TGG	0.534418	5_2:-427347	Msa0747650:CDS
TGAAGTGTGAGATTAGTGTC+AGG	0.535569	5_2:+430260	None:intergenic
GATTATTCAAAACAAATGAC+TGG	0.535856	5_2:+426499	None:intergenic
TCGTGTACGGTTGACATTGG+AGG	0.535923	5_2:-427887	Msa0747650:CDS
AACGGAATCCATTGCTGATG+AGG	0.536315	5_2:-427714	Msa0747650:CDS
TATCCAGACAAATGCATCCC+AGG	0.536475	5_2:-427789	Msa0747650:CDS
CAAGAGTATGCACAGAAAGC+AGG	0.538168	5_2:-428855	Msa0747650:CDS
CATCGCCAATATGTATCCCT+CGG	0.539377	5_2:+426389	None:intergenic
TTCTGCATGGCTTCATCTGG+AGG	0.540711	5_2:+427191	None:intergenic
TTTGTCATGGCTTCATCTGA+AGG	0.542263	5_2:+426771	None:intergenic
CGTGTACGGTTGACATTGGA+GGG	0.543141	5_2:-427886	Msa0747650:CDS
AGAGACATTAGCTGGCATCC+AGG	0.543604	5_2:-426610	Msa0747650:CDS
ATGAACAAACAGAATTGCAA+TGG	0.547564	5_2:-426357	Msa0747650:CDS
GAATTCTTCATGGCTTCTGA+AGG	0.548362	5_2:+427293	None:intergenic
GGGATGGAATGACAGTCAAA+TGG	0.548556	5_2:+427747	None:intergenic
ATCGCCAATATGTATCCCTC+GGG	0.552320	5_2:+426390	None:intergenic
GGATGACACACCACCAGGTA+GGG	0.554430	5_2:-427921	Msa0747650:CDS
GGCACCATTATATTCGTGTA+CGG	0.559813	5_2:-427900	Msa0747650:CDS
GGAGGGATGCTCTATATTGG+AGG	0.559971	5_2:-427869	Msa0747650:CDS
ATCTTCGTTAGTGGTGGACA+TGG	0.562155	5_2:+429928	None:intergenic
AACACTGACAATGCTGGTGA+TGG	0.565998	5_2:-427605	Msa0747650:CDS
AGAAGTGTCATTTGCTCCCG+AGG	0.566950	5_2:-426406	Msa0747650:CDS
AACGGCGCAGATTCCGACAC+AGG	0.569849	5_2:-430058	Msa0747650:five_prime_UTR
GTGTATGAAACTACTAAGGA+AGG	0.573469	5_2:-428819	Msa0747650:CDS
TATGCACAGAAAGCAGGACT+TGG	0.576788	5_2:-428849	Msa0747650:CDS
TAATACTGCATGGCTTCCGA+AGG	0.576922	5_2:+427395	None:intergenic
TCCATTATCATAATACTGCA+TGG	0.577735	5_2:+427385	None:intergenic
TTGGAAATCTTCGTTAGTGG+TGG	0.577745	5_2:+429922	None:intergenic
AATTCTTCATGACTTCTGAA+AGG	0.581745	5_2:+426565	None:intergenic
GAGAAGATGAAATTGAAGAG+TGG	0.587475	5_2:+429963	None:intergenic
CTTCAGATTTGATTTCCTGG+AGG	0.587651	5_2:+427426	None:intergenic
CTTCAGATTTGATTTCCTGG+AGG	0.587651	5_2:+427528	None:intergenic
AGTGGTGGACATGGTGAGAG+AGG	0.590480	5_2:+429937	None:intergenic
GCCATGCAGAATTCTGAGAA+CGG	0.591866	5_2:-426969	Msa0747650:CDS
TTGTCATGGCTTCATCTGAA+GGG	0.594389	5_2:+426772	None:intergenic
TGACCTGAAAGAAGGTTCAT+GGG	0.594942	5_2:+428791	None:intergenic
CCCGTTCTCAGAATTCTGCA+TGG	0.596419	5_2:+426968	None:intergenic
TCCGTTCTCAGAATTCTGCA+TGG	0.602147	5_2:+427073	None:intergenic
CTGAAAGAAGGTTCATGGGA+AGG	0.602671	5_2:+428795	None:intergenic
TTTCGAAGCCTCATCAGCAA+TGG	0.604541	5_2:+427706	None:intergenic
GCGATGCAGAATTCTGAGAA+CGG	0.606253	5_2:-426864	Msa0747650:CDS
TCCGTTCTCAGAATTCTGCA+TGG	0.609171	5_2:+427178	None:intergenic
AGGATGACACACCACCAGGT+AGG	0.617789	5_2:-427922	Msa0747650:CDS
TCTGCATGGCTTCATCTGAA+GGG	0.618565	5_2:+426982	None:intergenic
TCTGCATGGCTTCATCTGAA+GGG	0.618565	5_2:+427087	None:intergenic
GAAGTGTCATTTGCTCCCGA+GGG	0.622643	5_2:-426405	Msa0747650:CDS
TGAGAACAAAGTTGTGTGTA+CGG	0.623105	5_2:+430030	None:intergenic
CTCCAGATTTGACTTCCTGG+AGG	0.623620	5_2:+427324	None:intergenic
ATACATATTGGCGATGAGCG+TGG	0.624072	5_2:-426382	Msa0747650:CDS
AGCTAATAAAGCAGTTCTCG+CGG	0.626972	5_2:+427811	None:intergenic
GCCATGAAGAATTCTGAGAA+CGG	0.628543	5_2:-427284	Msa0747650:CDS
TCTGCATGGCTTCATCTGGA+GGG	0.629877	5_2:+427192	None:intergenic
AGAGACGTTAGCTACCATCC+AGG	0.630811	5_2:-426922	Msa0747650:CDS
AATTCGTCATGGCTTCTGAA+CGG	0.635308	5_2:+426667	None:intergenic
TCTGTTCTCAGAATTCGTCA+TGG	0.636980	5_2:+426656	None:intergenic
GCCATGCAGAATTCTGAGAA+CGG	0.638030	5_2:-427074	Msa0747650:CDS
GCCATGCAGAATTCTGAGAA+CGG	0.638440	5_2:-427179	Msa0747650:CDS
GTGTACGGTGAGGCCTGTGT+CGG	0.646839	5_2:+430045	None:intergenic
TGTATGAAACTACTAAGGAA+GGG	0.648493	5_2:-428818	Msa0747650:CDS
AATACTGCATGGCTTCCGAA+GGG	0.654813	5_2:+427396	None:intergenic
ACCACTAACGAAGATTTCCA+AGG	0.657994	5_2:-429920	Msa0747650:intron
CTTCAGATTTAACTTCCTGG+AGG	0.663895	5_2:+427222	None:intergenic
CATGCAGAATTCTGAGAACG+GGG	0.674016	5_2:-426967	Msa0747650:CDS
TGGAGCAAAGATCAATAATG+GGG	0.696605	5_2:-427585	Msa0747650:CDS
ACAAAGTTGTGTGTACGGTG+AGG	0.701059	5_2:+430035	None:intergenic
GATGCAGAATTCTGAGAACG+GGG	0.733105	5_2:-426862	Msa0747650:CDS
TATAATGGTGCCCTACCTGG+TGG	0.785345	5_2:+427911	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATCGTATATTATTTATTT+TGG	+	chr5_2:426464-426483	None:intergenic	10.0%
!!!	ACAAATTATAATTTTTTTGT+TGG	-	chr5_2:427991-428010	Msa0747650:CDS	10.0%
!!	ATCTAAATTAAGAATATTTC+TGG	+	chr5_2:426409-426428	None:intergenic	15.0%
!!	ACAAAAATTAAACTTTCATA+GGG	-	chr5_2:427608-427627	Msa0747650:CDS	15.0%
!!!	ATATTTGAAGTTTTTATTGT+GGG	-	chr5_2:428082-428101	Msa0747650:intron	15.0%
!!!	TATTTTCAGAAGAATAATAT+TGG	+	chr5_2:428328-428347	None:intergenic	15.0%
!!	AATCAAAGATCAAGTATTAT+GGG	+	chr5_2:426834-426853	None:intergenic	20.0%
!!	CAACGAGAATTATATTAAAT+CGG	-	chr5_2:427374-427393	Msa0747650:CDS	20.0%
!!	GACAAAAATTAAACTTTCAT+AGG	-	chr5_2:427607-427626	Msa0747650:CDS	20.0%
!!!	TATTTGCTTTCTTTTTTAAG+TGG	-	chr5_2:427689-427708	Msa0747650:CDS	20.0%
!!!	GATATTTGAAGTTTTTATTG+TGG	-	chr5_2:428081-428100	Msa0747650:intron	20.0%
!!!	GATAATTTGTATGCAGTTTT+TGG	+	chr5_2:426614-426633	None:intergenic	25.0%
!!!	ATATAAAATAGAAGCATTGG+TGG	+	chr5_2:426810-426829	None:intergenic	25.0%
!!	GGAATATAAAATAGAAGCAT+TGG	+	chr5_2:426813-426832	None:intergenic	25.0%
!	CAATCAAAGATCAAGTATTA+TGG	+	chr5_2:426835-426854	None:intergenic	25.0%
!!!	TGATCTTTGATTGTTTTGAA+TGG	-	chr5_2:426863-426882	Msa0747650:CDS	25.0%
!!!	GATCTTTGATTGTTTTGAAT+GGG	-	chr5_2:426864-426883	Msa0747650:CDS	25.0%
!	TTTCAGTTATGATTCATACT+CGG	-	chr5_2:427138-427157	Msa0747650:CDS	25.0%
!!	TTTGAAAATTGACTCTAGTA+AGG	-	chr5_2:427300-427319	Msa0747650:CDS	25.0%
!	CGATTTAATATAATTCTCGT+TGG	+	chr5_2:427376-427395	None:intergenic	25.0%
!	ACAAGTAAATAGTTTATCCT+TGG	-	chr5_2:427409-427428	Msa0747650:CDS	25.0%
!	ATGTGAAAAATAATCTGCAA+CGG	+	chr5_2:428045-428064	None:intergenic	25.0%
!	CATCTCATTATAAACTATCA+AGG	+	chr5_2:428429-428448	None:intergenic	25.0%
!	ACTGTTGCATATTATATTTG+AGG	-	chr5_2:428456-428475	Msa0747650:intron	25.0%
!	TATCTAGTGAAAATCGAATA+GGG	+	chr5_2:428484-428503	None:intergenic	25.0%
!	ATATCTAGTGAAAATCGAAT+AGG	+	chr5_2:428485-428504	None:intergenic	25.0%
!	TATGAATACAGAACTTAGTA+AGG	+	chr5_2:428513-428532	None:intergenic	25.0%
!!!	TCTGGTTCTGATTATTTTAA+TGG	-	chr5_2:428565-428584	Msa0747650:intron	25.0%
!!	AATTTTTCATGACTTCTGAA+GGG	+	chr5_2:429110-429129	None:intergenic	25.0%
!	GATTATTCAAAACAAATGAC+TGG	+	chr5_2:430109-430128	None:intergenic	25.0%
!!!	AATCAAGAGAATTTTGAAAG+TGG	-	chr5_2:430125-430144	Msa0747650:five_prime_UTR	25.0%
!!!	ATCAAGAGAATTTTGAAAGT+GGG	-	chr5_2:430126-430145	Msa0747650:five_prime_UTR	25.0%
!	AATCCAAAGAGATCAATATT+GGG	-	chr5_2:430165-430184	Msa0747650:five_prime_UTR	25.0%
!	CACTTTTCATAGAATCACAA+CGG	-	chr5_2:426529-426548	Msa0747650:CDS	30.0%
	GAGAATAATAGTTAGTACCT+TGG	+	chr5_2:426705-426724	None:intergenic	30.0%
!	CTTTTTCTTTTACTTTCCCA+GGG	-	chr5_2:426728-426747	Msa0747650:CDS	30.0%
	TGATTCATCTTATAGAGTGA+TGG	-	chr5_2:427001-427020	Msa0747650:CDS	30.0%
!	TATAGAGTGATGGAAGATAA+AGG	-	chr5_2:427011-427030	Msa0747650:CDS	30.0%
!	ATAGAGTGATGGAAGATAAA+GGG	-	chr5_2:427012-427031	Msa0747650:CDS	30.0%
!!	CTTTTTGGTCTTTAACCTTT+TGG	-	chr5_2:427105-427124	Msa0747650:CDS	30.0%
	TGAAACAATTACAGACCAAA+AGG	+	chr5_2:427123-427142	None:intergenic	30.0%
	TTCAGTTATGATTCATACTC+GGG	-	chr5_2:427139-427158	Msa0747650:CDS	30.0%
!	GTGAACGATTATTTCTTAAC+TGG	-	chr5_2:427166-427185	Msa0747650:CDS	30.0%
	ACTACTTAAGTTCAATCACT+TGG	-	chr5_2:427198-427217	Msa0747650:CDS	30.0%
!	AAAATTGACTCTAGTAAGGA+GGG	-	chr5_2:427304-427323	Msa0747650:CDS	30.0%
	CTCTCTAAATGTCTAACTAT+TGG	+	chr5_2:427352-427371	None:intergenic	30.0%
	TAAATAGTTTATCCTTGGAC+AGG	-	chr5_2:427414-427433	Msa0747650:CDS	30.0%
	AAATAGTTTATCCTTGGACA+GGG	-	chr5_2:427415-427434	Msa0747650:CDS	30.0%
	ACCTTAGAGAAAACCTAAAT+CGG	+	chr5_2:427569-427588	None:intergenic	30.0%
	AAATTAAACTTTCATAGGGC+AGG	-	chr5_2:427612-427631	Msa0747650:CDS	30.0%
	TTAAGTCAGATGTTGAATGA+AGG	+	chr5_2:427651-427670	None:intergenic	30.0%
!!!	TGCTTTCTTTTTTAAGTGGT+AGG	-	chr5_2:427693-427712	Msa0747650:CDS	30.0%
	TGTATGAAACTACTAAGGAA+GGG	-	chr5_2:427787-427806	Msa0747650:CDS	30.0%
	CAGTGATTATGAATGATGTT+CGG	-	chr5_2:427838-427857	Msa0747650:CDS	30.0%
	TTGTAAAGACTACTTACAAC+AGG	+	chr5_2:427975-427994	None:intergenic	30.0%
	AAGTTGAAAGTAGAAAAGAC+AGG	+	chr5_2:428115-428134	None:intergenic	30.0%
	TTAGCTAATCAACGTAAAGA+TGG	+	chr5_2:428159-428178	None:intergenic	30.0%
!	TCTTTACGTTGATTAGCTAA+TGG	-	chr5_2:428159-428178	Msa0747650:intron	30.0%
!!	CATTGTTTTGTTGATGTGAT+TGG	-	chr5_2:428222-428241	Msa0747650:intron	30.0%
!	GCGAGTTATTGTATATTTTC+TGG	-	chr5_2:428547-428566	Msa0747650:intron	30.0%
	ATTGTGCAAGAATTTACTTC+AGG	-	chr5_2:428612-428631	Msa0747650:intron	30.0%
	TCTAAGAAATCACGCTTTAA+AGG	-	chr5_2:428931-428950	Msa0747650:intron	30.0%
!	CTAAGAAATCACGCTTTAAA+GGG	-	chr5_2:428932-428951	Msa0747650:intron	30.0%
	ATTTCTATGTGGTTTCTTCT+TGG	+	chr5_2:428965-428984	None:intergenic	30.0%
	ATGGAGCAAAGATCAATAAT+GGG	-	chr5_2:429019-429038	Msa0747650:intron	30.0%
!	GAATTTTTCATGACTTCTGA+AGG	+	chr5_2:429111-429130	None:intergenic	30.0%
!	GTCATGAAAAATTCTGAGAA+CGG	-	chr5_2:429117-429136	Msa0747650:intron	30.0%
	TCCATTATCATAATACTGCA+TGG	+	chr5_2:429223-429242	None:intergenic	30.0%
	TGCAATAAAGGAAGTCAAAT+CGG	-	chr5_2:429485-429504	Msa0747650:intron	30.0%
	AATTCTTCATGACTTCTGAA+AGG	+	chr5_2:430043-430062	None:intergenic	30.0%
	CAATCCAAAGAGATCAATAT+TGG	-	chr5_2:430164-430183	Msa0747650:five_prime_UTR	30.0%
	ATTCCCAATATTGATCTCTT+TGG	+	chr5_2:430171-430190	None:intergenic	30.0%
	ATGAACAAACAGAATTGCAA+TGG	-	chr5_2:430248-430267	Msa0747650:five_prime_UTR	30.0%
	TGAGAACAAAGTTGTGTGTA+CGG	+	chr5_2:426578-426597	None:intergenic	35.0%
	GATGAAATTGAAGAGTGGTT+GGG	+	chr5_2:426640-426659	None:intergenic	35.0%
	AGATGAAATTGAAGAGTGGT+TGG	+	chr5_2:426641-426660	None:intergenic	35.0%
	GAGAAGATGAAATTGAAGAG+TGG	+	chr5_2:426645-426664	None:intergenic	35.0%
	CCTGGGAAAGTAAAAGAAAA+AGG	+	chr5_2:426730-426749	None:intergenic	35.0%
!	CCTTTTTCTTTTACTTTCCC+AGG	-	chr5_2:426727-426746	Msa0747650:CDS	35.0%
!!	TAAACTTGTTGCAACGATCT+AGG	-	chr5_2:426958-426977	Msa0747650:CDS	35.0%
!	AATCAAGTGTTTCTGTCCTA+GGG	+	chr5_2:426986-427005	None:intergenic	35.0%
!	AGTTCAATCACTTGGTTTGA+AGG	-	chr5_2:427206-427225	Msa0747650:CDS	35.0%
!	GTTCAATCACTTGGTTTGAA+GGG	-	chr5_2:427207-427226	Msa0747650:CDS	35.0%
!	TTCAATCACTTGGTTTGAAG+GGG	-	chr5_2:427208-427227	Msa0747650:CDS	35.0%
!	GAAAATTGACTCTAGTAAGG+AGG	-	chr5_2:427303-427322	Msa0747650:CDS	35.0%
!!	TAAGGAGGGTTGATGATTAA+CGG	-	chr5_2:427318-427337	Msa0747650:CDS	35.0%
!!!	CTTTTTTCACAAAGTTGGGT+CGG	+	chr5_2:427467-427486	None:intergenic	35.0%
!!!	GAGCCTTTTTTCACAAAGTT+GGG	+	chr5_2:427471-427490	None:intergenic	35.0%
!!!	TGAGCCTTTTTTCACAAAGT+TGG	+	chr5_2:427472-427491	None:intergenic	35.0%
	TCCTGAAATAGATGCATTCT+AGG	-	chr5_2:427510-427529	Msa0747650:CDS	35.0%
	TCCTAGAATGCATCTATTTC+AGG	+	chr5_2:427514-427533	None:intergenic	35.0%
	GCTTCTCAAATCTTACATCT+TGG	+	chr5_2:427537-427556	None:intergenic	35.0%
	AAGATTTGAGAAGCAACAAG+TGG	-	chr5_2:427543-427562	Msa0747650:CDS	35.0%
	ACCTGTGTATGAAACTACTA+AGG	-	chr5_2:427782-427801	Msa0747650:CDS	35.0%
	TCCTTAGTAGTTTCATACAC+AGG	+	chr5_2:427786-427805	None:intergenic	35.0%
	GTGTATGAAACTACTAAGGA+AGG	-	chr5_2:427786-427805	Msa0747650:CDS	35.0%
	TTCACATTTCTTCAGCTCAT+TGG	-	chr5_2:428058-428077	Msa0747650:intron	35.0%
	TCACATTTCTTCAGCTCATT+GGG	-	chr5_2:428059-428078	Msa0747650:intron	35.0%
	CTACTTTCAACTTTCAAGAG+TGG	-	chr5_2:428122-428141	Msa0747650:intron	35.0%
	GTTCTTAGTATGCAACAGTT+TGG	-	chr5_2:428195-428214	Msa0747650:intron	35.0%
!	TCCTTTTTGCTCTGATACAA+TGG	+	chr5_2:428667-428686	None:intergenic	35.0%
	TCAACCGTACACGAATATAA+TGG	+	chr5_2:428712-428731	None:intergenic	35.0%
	AGGAACAGCAAAAACAAAGA+GGG	-	chr5_2:428756-428775	Msa0747650:CDS	35.0%
	AAGCGTGATTTCTTAGACTT+CGG	+	chr5_2:428928-428947	None:intergenic	35.0%
	CAGCATTGTCAGTGTTAATA+CGG	+	chr5_2:428996-429015	None:intergenic	35.0%
	GATGGAGCAAAGATCAATAA+TGG	-	chr5_2:429018-429037	Msa0747650:intron	35.0%
	TGGAGCAAAGATCAATAATG+GGG	-	chr5_2:429020-429039	Msa0747650:intron	35.0%
	GCCATGCAGTATTATGATAA+TGG	-	chr5_2:429219-429238	Msa0747650:intron	35.0%
	AGAGACATTAGCTGCAATAA+AGG	-	chr5_2:429473-429492	Msa0747650:intron	35.0%
!	TTCAGATTTGATTTCCTGGA+TGG	+	chr5_2:429079-429098	None:intergenic	35.0%
!	TTCAGATTTGATTTCCTGGA+TGG	+	chr5_2:429079-429098	None:intergenic	35.0%
!	GCCATGACAAATTCTGAAAA+CGG	-	chr5_2:429846-429865	Msa0747650:intron	35.0%
!	TCCGTTTTCAGAATTTGTCA+TGG	+	chr5_2:429850-429869	None:intergenic	35.0%
	TCAGAAGTCATGAAGAATTC+TGG	-	chr5_2:430044-430063	Msa0747650:five_prime_UTR	35.0%
	CAGAAGTCATGAAGAATTCT+GGG	-	chr5_2:430045-430064	Msa0747650:five_prime_UTR	35.0%
	ACAGAATTGCAATGGAGATA+TGG	-	chr5_2:430256-430275	Msa0747650:five_prime_UTR	35.0%
	TGAAGTGTGAGATTAGTGTC+AGG	+	chr5_2:426348-426367	None:intergenic	40.0%
	TTGGAAATCTTCGTTAGTGG+TGG	+	chr5_2:426686-426705	None:intergenic	40.0%
	ACCACTAACGAAGATTTCCA+AGG	-	chr5_2:426685-426704	Msa0747650:CDS	40.0%
	ACCTTGGAAATCTTCGTTAG+TGG	+	chr5_2:426689-426708	None:intergenic	40.0%
!	GAATCAAGTGTTTCTGTCCT+AGG	+	chr5_2:426987-427006	None:intergenic	40.0%
	TGGAGGGAGGAAAAAAAATG+AGG	-	chr5_2:427047-427066	Msa0747650:CDS	40.0%
	GGAGGGAGGAAAAAAAATGA+GGG	-	chr5_2:427048-427067	Msa0747650:CDS	40.0%
!	GAGGGGTCAGAATTTCTTTT+TGG	-	chr5_2:427090-427109	Msa0747650:CDS	40.0%
	CGACCCAACTTTGTGAAAAA+AGG	-	chr5_2:427465-427484	Msa0747650:CDS	40.0%
	CTTTGTGAAAAAAGGCTCAG+TGG	-	chr5_2:427473-427492	Msa0747650:CDS	40.0%
!!	GCCGATTTAGGTTTTCTCTA+AGG	-	chr5_2:427565-427584	Msa0747650:CDS	40.0%
	TCTGTGCATACTCTTGCAAT+TGG	+	chr5_2:427746-427765	None:intergenic	40.0%
	TGACCTGAAAGAAGGTTCAT+GGG	+	chr5_2:427817-427836	None:intergenic	40.0%
	TTGACCTGAAAGAAGGTTCA+TGG	+	chr5_2:427818-427837	None:intergenic	40.0%
	ATCACTGTTGACCTGAAAGA+AGG	+	chr5_2:427825-427844	None:intergenic	40.0%
	GTTCGGTATGATTCTTTGAC+TGG	-	chr5_2:427855-427874	Msa0747650:CDS	40.0%
	TTGATGGAGTTGGCTAAAAC+TGG	-	chr5_2:427924-427943	Msa0747650:CDS	40.0%
!!	TAATATTGGTGACGAGCTCA+CGG	+	chr5_2:428314-428333	None:intergenic	40.0%
!	GTTTTCTTCCAGCATGAAAC+TGG	-	chr5_2:428589-428608	Msa0747650:intron	40.0%
	GCCATTGTATCAGAGCAAAA+AGG	-	chr5_2:428663-428682	Msa0747650:CDS	40.0%
	GGCACCATTATATTCGTGTA+CGG	-	chr5_2:428705-428724	Msa0747650:CDS	40.0%
	TATTCGTGTACGGTTGACAT+TGG	-	chr5_2:428715-428734	Msa0747650:CDS	40.0%
	ATTGGAGGGATGCTCTATAT+TGG	-	chr5_2:428733-428752	Msa0747650:CDS	40.0%
	GAGGAACAGCAAAAACAAAG+AGG	-	chr5_2:428755-428774	Msa0747650:CDS	40.0%
	AGCTAATAAAGCAGTTCTCG+CGG	+	chr5_2:428797-428816	None:intergenic	40.0%
!!!	AAACTGGTTTTCAGAAGCCT+GGG	+	chr5_2:428836-428855	None:intergenic	40.0%
	TGTCATTCCATCCCGAAAAA+GGG	-	chr5_2:428867-428886	Msa0747650:CDS	40.0%
	CGTATTAACACTGACAATGC+TGG	-	chr5_2:428994-429013	Msa0747650:intron	40.0%
!	CAATAATGGGGAAGAATCAC+TGG	-	chr5_2:429032-429051	Msa0747650:intron	40.0%
	GCAAGTGCTAATGATGAATC+TGG	-	chr5_2:429054-429073	Msa0747650:intron	40.0%
!	CTTCAGATTTGATTTCCTGG+AGG	+	chr5_2:429080-429099	None:intergenic	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
	CATGAGAAAGAGACATTAGC+TGG	-	chr5_2:429156-429175	Msa0747650:intron	40.0%
!	CTTCAGATTTGATTTCCTGG+AGG	+	chr5_2:429080-429099	None:intergenic	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
	CATGAGAAAGAGACATTAGC+TGG	-	chr5_2:429156-429175	Msa0747650:intron	40.0%
	GAATTCTTCATGGCTTCTGA+AGG	+	chr5_2:429315-429334	None:intergenic	40.0%
!	GCCATGAAGAATTCTGAGAA+CGG	-	chr5_2:429321-429340	Msa0747650:intron	40.0%
	TCCGTTCTCAGAATTCTTCA+TGG	+	chr5_2:429325-429344	None:intergenic	40.0%
	CATGAGAAAGAGACATTAGC+TGG	-	chr5_2:429156-429175	Msa0747650:intron	40.0%
!	CTTCAGATTTAACTTCCTGG+AGG	+	chr5_2:429386-429405	None:intergenic	40.0%
!	CTGCTTCAGATTTAACTTCC+TGG	+	chr5_2:429389-429408	None:intergenic	40.0%
!	TTCAGATTTGACTTCCTGGA+TGG	+	chr5_2:429595-429614	None:intergenic	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
	TTGTCATGGCTTCATCTGAA+GGG	+	chr5_2:429836-429855	None:intergenic	40.0%
	TTTGTCATGGCTTCATCTGA+AGG	+	chr5_2:429837-429856	None:intergenic	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
	AATTCGTCATGGCTTCTGAA+CGG	+	chr5_2:429941-429960	None:intergenic	40.0%
	TCTGTTCTCAGAATTCGTCA+TGG	+	chr5_2:429952-429971	None:intergenic	40.0%
	CATGAGAAAGAGACATTAGC+TGG	-	chr5_2:429156-429175	Msa0747650:intron	40.0%
!	CTGCTTCAGATTTGATTTCC+TGG	+	chr5_2:429083-429102	None:intergenic	40.0%
	ACAAAGTTGTGTGTACGGTG+AGG	+	chr5_2:426573-426592	None:intergenic	45.0%
!!	ATCTTCGTTAGTGGTGGACA+TGG	+	chr5_2:426680-426699	None:intergenic	45.0%
	GCGTTGCAAAAACAAACCCT+GGG	+	chr5_2:426747-426766	None:intergenic	45.0%
	TGCGTTGCAAAAACAAACCC+TGG	+	chr5_2:426748-426767	None:intergenic	45.0%
!	GTGATGGAAGATAAAGGGAG+TGG	-	chr5_2:427017-427036	Msa0747650:CDS	45.0%
	ATGGAAGATAAAGGGAGTGG+AGG	-	chr5_2:427020-427039	Msa0747650:CDS	45.0%
	TGGAAGATAAAGGGAGTGGA+GGG	-	chr5_2:427021-427040	Msa0747650:CDS	45.0%
!!!	AGGGATTCCCTCACTTTTTG+TGG	-	chr5_2:427067-427086	Msa0747650:CDS	45.0%
!!!	GGGATTCCCTCACTTTTTGT+GGG	-	chr5_2:427068-427087	Msa0747650:CDS	45.0%
!!!	ATTCCCTCACTTTTTGTGGG+AGG	-	chr5_2:427071-427090	Msa0747650:CDS	45.0%
!!!	TTCCCTCACTTTTTGTGGGA+GGG	-	chr5_2:427072-427091	Msa0747650:CDS	45.0%
!	AATCACTTGGTTTGAAGGGG+TGG	-	chr5_2:427211-427230	Msa0747650:CDS	45.0%
!	TATCCTTGGACAGGGTTTTG+TGG	-	chr5_2:427423-427442	Msa0747650:CDS	45.0%
!	AAGCAACAAGTGGCCGATTT+AGG	-	chr5_2:427553-427572	Msa0747650:CDS	45.0%
!	CAAGAGTATGCACAGAAAGC+AGG	-	chr5_2:427750-427769	Msa0747650:CDS	45.0%
	TATGCACAGAAAGCAGGACT+TGG	-	chr5_2:427756-427775	Msa0747650:CDS	45.0%
	CTGAAAGAAGGTTCATGGGA+AGG	+	chr5_2:427813-427832	None:intergenic	45.0%
	CTTCCCATGAACCTTCTTTC+AGG	-	chr5_2:427811-427830	Msa0747650:CDS	45.0%
!	AGAGGTTGCTTTGATGGAGT+TGG	-	chr5_2:427914-427933	Msa0747650:CDS	45.0%
!!	AATATTGGTGACGAGCTCAC+GGG	+	chr5_2:428313-428332	None:intergenic	45.0%
	TGCACAATCCAGTTTCATGC+TGG	+	chr5_2:428600-428619	None:intergenic	45.0%
	AAAAAGGATGACACACCACC+AGG	-	chr5_2:428679-428698	Msa0747650:CDS	45.0%
	GAATATAATGGTGCCCTACC+TGG	+	chr5_2:428700-428719	None:intergenic	45.0%
	TATCCAGACAAATGCATCCC+AGG	-	chr5_2:428816-428835	Msa0747650:CDS	45.0%
!!!	CAAACTGGTTTTCAGAAGCC+TGG	+	chr5_2:428837-428856	None:intergenic	45.0%
!	CAGGCTTCTGAAAACCAGTT+TGG	-	chr5_2:428835-428854	Msa0747650:CDS	45.0%
	TGACAGTCAAATGGCCAAAC+TGG	+	chr5_2:428852-428871	None:intergenic	45.0%
	GGGATGGAATGACAGTCAAA+TGG	+	chr5_2:428861-428880	None:intergenic	45.0%
	CTGTCATTCCATCCCGAAAA+AGG	-	chr5_2:428866-428885	Msa0747650:CDS	45.0%
!	TGGATTCCGTTGCCCTTTTT+CGG	+	chr5_2:428882-428901	None:intergenic	45.0%
	AACGGAATCCATTGCTGATG+AGG	-	chr5_2:428891-428910	Msa0747650:CDS	45.0%
	TTTCGAAGCCTCATCAGCAA+TGG	+	chr5_2:428902-428921	None:intergenic	45.0%
!!	CGGCGCTTCTTATTTCTATG+TGG	+	chr5_2:428976-428995	None:intergenic	45.0%
	AACACTGACAATGCTGGTGA+TGG	-	chr5_2:429000-429019	Msa0747650:intron	45.0%
	TAATGATGAATCTGGCCTCC+AGG	-	chr5_2:429062-429081	Msa0747650:intron	45.0%
!	ATCTGAAGCAGCATTCCCTT+CGG	-	chr5_2:429092-429111	Msa0747650:intron	45.0%
	AATACTGCATGGCTTCCGAA+GGG	+	chr5_2:429212-429231	None:intergenic	45.0%
	TAATACTGCATGGCTTCCGA+AGG	+	chr5_2:429213-429232	None:intergenic	45.0%
	GAATTCTGCATGGCTTCATC+TGG	+	chr5_2:429420-429439	None:intergenic	45.0%
!	GCCATGCAGAATTCTGAGAA+CGG	-	chr5_2:429426-429445	Msa0747650:intron	45.0%
	TCCGTTCTCAGAATTCTGCA+TGG	+	chr5_2:429430-429449	None:intergenic	45.0%
	TCTGCATGGCTTCATCTGAA+GGG	+	chr5_2:429521-429540	None:intergenic	45.0%
	TTCTGCATGGCTTCATCTGA+AGG	+	chr5_2:429522-429541	None:intergenic	45.0%
!	GCCATGCAGAATTCTGAGAA+CGG	-	chr5_2:429426-429445	Msa0747650:intron	45.0%
	TCCGTTCTCAGAATTCTGCA+TGG	+	chr5_2:429430-429449	None:intergenic	45.0%
	AGAAACATTAGCTGCCATCC+AGG	-	chr5_2:429578-429597	Msa0747650:intron	45.0%
!	CTGCTTCAGATTTGACTTCC+TGG	+	chr5_2:429599-429618	None:intergenic	45.0%
	TCTGCATGGCTTCATCTGAA+GGG	+	chr5_2:429521-429540	None:intergenic	45.0%
	TTCTGCATGGCTTCATCTGA+AGG	+	chr5_2:429522-429541	None:intergenic	45.0%
!	GCCATGCAGAATTCTGAGAA+CGG	-	chr5_2:429426-429445	Msa0747650:intron	45.0%
!	CCATGCAGAATTCTGAGAAC+GGG	-	chr5_2:429427-429446	Msa0747650:intron	45.0%
!	CATGCAGAATTCTGAGAACG+GGG	-	chr5_2:429428-429447	Msa0747650:intron	45.0%
	TTCTGCATCGCTTCATCTGA+AGG	+	chr5_2:429732-429751	None:intergenic	45.0%
!	GCGATGCAGAATTCTGAGAA+CGG	-	chr5_2:429741-429760	Msa0747650:intron	45.0%
!	CGATGCAGAATTCTGAGAAC+GGG	-	chr5_2:429742-429761	Msa0747650:intron	45.0%
!	GATGCAGAATTCTGAGAACG+GGG	-	chr5_2:429743-429762	Msa0747650:intron	45.0%
	ATCGCCAATATGTATCCCTC+GGG	+	chr5_2:430218-430237	None:intergenic	45.0%
	CATCGCCAATATGTATCCCT+CGG	+	chr5_2:430219-430238	None:intergenic	45.0%
	ATACATATTGGCGATGAGCG+TGG	-	chr5_2:430223-430242	Msa0747650:five_prime_UTR	45.0%
	TGCAATGGAGATATGGTCTC+TGG	-	chr5_2:430263-430282	Msa0747650:five_prime_UTR	45.0%
	TGCAACGATCTAGGAACCCT+AGG	-	chr5_2:426967-426986	Msa0747650:CDS	50.0%
	ATAAAGGGAGTGGAGGGCTT+TGG	-	chr5_2:427027-427046	Msa0747650:CDS	50.0%
!!!	TCCCTCACTTTTTGTGGGAG+GGG	-	chr5_2:427073-427092	Msa0747650:CDS	50.0%
	ACCCCTCCCACAAAAAGTGA+GGG	+	chr5_2:427077-427096	None:intergenic	50.0%
	AAGCCACAAAACCCTGTCCA+AGG	+	chr5_2:427429-427448	None:intergenic	50.0%
!	AGCTGCAGAGGTTGCTTTGA+TGG	-	chr5_2:427908-427927	Msa0747650:CDS	50.0%
	TCTGCAACGGTCACTACACT+CGG	+	chr5_2:428032-428051	None:intergenic	50.0%
	TATAATGGTGCCCTACCTGG+TGG	+	chr5_2:428697-428716	None:intergenic	50.0%
	TCGTGTACGGTTGACATTGG+AGG	-	chr5_2:428718-428737	Msa0747650:CDS	50.0%
	CGTGTACGGTTGACATTGGA+GGG	-	chr5_2:428719-428738	Msa0747650:CDS	50.0%
	GGAGGGATGCTCTATATTGG+AGG	-	chr5_2:428736-428755	Msa0747650:CDS	50.0%
	AAGCCTGGGATGCATTTGTC+TGG	+	chr5_2:428822-428841	None:intergenic	50.0%
	TCCATCCCGAAAAAGGGCAA+CGG	-	chr5_2:428873-428892	Msa0747650:CDS	50.0%
!	GGATTCCGTTGCCCTTTTTC+GGG	+	chr5_2:428881-428900	None:intergenic	50.0%
!	CTCCAGATTTGACTTCCTGG+AGG	+	chr5_2:429284-429303	None:intergenic	50.0%
!	CTGCTCCAGATTTGACTTCC+TGG	+	chr5_2:429287-429306	None:intergenic	50.0%
	TCTGCATGGCTTCATCTGGA+GGG	+	chr5_2:429416-429435	None:intergenic	50.0%
	TTCTGCATGGCTTCATCTGG+AGG	+	chr5_2:429417-429436	None:intergenic	50.0%
	CCCGTTCTCAGAATTCTGCA+TGG	+	chr5_2:429640-429659	None:intergenic	50.0%
	AGAGACGTTAGCTACCATCC+AGG	-	chr5_2:429683-429702	Msa0747650:intron	50.0%
	AGAGACATTAGCTGCCATCC+AGG	-	chr5_2:429788-429807	Msa0747650:intron	50.0%
	AGAGACATTAGCTGCAGTCC+AGG	-	chr5_2:429893-429912	Msa0747650:intron	50.0%
	AGAGACATTAGCTGGCATCC+AGG	-	chr5_2:429995-430014	Msa0747650:five_prime_UTR	50.0%
!!	AGAAGTGTCATTTGCTCCCG+AGG	-	chr5_2:430199-430218	Msa0747650:five_prime_UTR	50.0%
!!	GAAGTGTCATTTGCTCCCGA+GGG	-	chr5_2:430200-430219	Msa0747650:five_prime_UTR	50.0%
	TGCTCCCGAGGGATACATAT+TGG	-	chr5_2:430211-430230	Msa0747650:five_prime_UTR	50.0%
!!	AGTGGTGGACATGGTGAGAG+AGG	+	chr5_2:426671-426690	None:intergenic	55.0%
	GACCCCTCCCACAAAAAGTG+AGG	+	chr5_2:427078-427097	None:intergenic	55.0%
	TGACGAGCTCACGGGTAAGA+AGG	+	chr5_2:428305-428324	None:intergenic	55.0%
	AGGATGACACACCACCAGGT+AGG	-	chr5_2:428683-428702	Msa0747650:CDS	55.0%
	GGATGACACACCACCAGGTA+GGG	-	chr5_2:428684-428703	Msa0747650:CDS	55.0%
!!!	TCCGTTGCCCTTTTTCGGGA+TGG	+	chr5_2:428877-428896	None:intergenic	55.0%
!	AGAGACATTAGCTGGCCTCC+AGG	-	chr5_2:429164-429183	Msa0747650:intron	55.0%
!	AGAGACATTAGCTGGCCTCC+AGG	-	chr5_2:429164-429183	Msa0747650:intron	55.0%
	GGCCTCCAGGAAGTCAAATC+TGG	-	chr5_2:429279-429298	Msa0747650:intron	55.0%
!	AGAGACATTAGCTGGCCTCC+AGG	-	chr5_2:429164-429183	Msa0747650:intron	55.0%
	AACGGCGCAGATTCCGACAC+AGG	-	chr5_2:426547-426566	Msa0747650:CDS	60.0%
	GTGTACGGTGAGGCCTGTGT+CGG	+	chr5_2:426563-426582	None:intergenic	60.0%
!	AAGGGAGTGGAGGGCTTTGG+AGG	-	chr5_2:427030-427049	Msa0747650:CDS	60.0%
!!	AGGGAGTGGAGGGCTTTGGA+GGG	-	chr5_2:427031-427050	Msa0747650:CDS	60.0%
	AGCAGAGCAGTCAGCTGCAG+AGG	-	chr5_2:427896-427915	Msa0747650:CDS	60.0%
!!	GAGTGGAGGGCTTTGGAGGG+AGG	-	chr5_2:427034-427053	Msa0747650:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	426335	430292	426335	ID=Msa0747650;Name=Msa0747650
chr5_2	mRNA	426335	430292	426335	ID=Msa0747650-mRNA-1;Parent=Msa0747650;Name=Msa0747650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=344\|1\|0.66\|1\|0.5\|0.66\|3\|0\|662
chr5_2	exon	426335	428026	426335	ID=Msa0747650-mRNA-1:exon:70;Parent=Msa0747650-mRNA-1
chr5_2	exon	428650	428918	428650	ID=Msa0747650-mRNA-1:exon:69;Parent=Msa0747650-mRNA-1
chr5_2	exon	429921	430292	429921	ID=Msa0747650-mRNA-1:exon:68;Parent=Msa0747650-mRNA-1
chr5_2	five_prime_UTR	429949	430292	429949	ID=Msa0747650-mRNA-1:five_prime_utr;Parent=Msa0747650-mRNA-1
chr5_2	CDS	429921	429948	429921	ID=Msa0747650-mRNA-1:cds;Parent=Msa0747650-mRNA-1
chr5_2	CDS	428650	428918	428650	ID=Msa0747650-mRNA-1:cds;Parent=Msa0747650-mRNA-1
chr5_2	CDS	426335	428026	426335	ID=Msa0747650-mRNA-1:cds;Parent=Msa0747650-mRNA-1

Gene Sequence

>Msa0747650

ATGTCCACCACTAACGAAGATTTCCAAGTGGTAGGTGTTTCAAATTGTTATGTGTTCAAGAGCCAATTGCAAGAGTATGCACAGAAAGCAGGACTTGGCACACCTGTGTATGAAACTACTAAGGAAGGGCCTTCCCATGAACCTTCTTTCAGGTCAACAGTGATTATGAATGATGTTCGGTATGATTCTTTGACTGGATTTTTTAATCGTAAAGCAGCAGAGCAGTCAGCTGCAGAGGTTGCTTTGATGGAGTTGGCTAAAACTGGTGAAGTTAATCAGTCTATTACTCAACCTGTTCATGAAACTGGATTGTGCAAGAATTTACTTCAGGAATATGCACAGAAAATGAATTATGCAATGCCATTGTATCAGAGCAAAAAGGATGACACACCACCAGGTAGGGCACCATTATATTCGTGTACGGTTGACATTGGAGGGATGCTCTATATTGGAGGAACAGCAAAAACAAAGAGGGAAGCTGAGATAAAAGCCGCGAGAACTGCTTTATTAGCTATCCAGACAAATGCATCCCAGGCTTCTGAAAACCAGTTTGGCCATTTGACTGTCATTCCATCCCGAAAAAGGGCAACGGAATCCATTGCTGATGAGGCTTCGAAAGCTCCGAAGTCTAAGAAATCACGCTTTAAAGGGAAATATTCCAAGAAGAAACCACATAGAAATAAGAAGCGCCGTATTAACACTGACAATGCTGGTGATGGAGCAAAGATCAATAATGGGGAAGAATCACTGGCAAGTGCTAATGATGAATCTGGCCTCCAGGAAATCAAATCTGAAGCAGCATTCCCTTCAGAAGTCATGAAAAATTCTGAGAACGGAGTATCATCTAATCATCATGAGAAAGAGACATTAGCTGGCCTCCAGGAAATCAAATCTGAAGCAGCATTCCCTTCGGAAGCCATGCAGTATTATGATAATGGAGTATCAACTAATCGTCATGAGAAAGAGACATTAGCTGGCCTCCAGGAAGTCAAATCTGGAGCAGCATTTCCTTCAGAAGCCATGAAGAATTCTGAGAACGGAGCATCAACTAATTATCATGAGAAAGAGACATTAGCTGGCCTCCAGGAAGTTAAATCTGAAGCAGCATTCCCTCCAGATGAAGCCATGCAGAATTCTGAGAACGGAGTGTCAACTAATCATCATGAGAAAGAGACATTAGCTGCAATAAAGGAAGTCAAATCGGAAGCAGCATTCCCTTCAGATGAAGCCATGCAGAATTCTGAGAACGGAGCATCAACTAATTATCATGAGAAAGAAACATTAGCTGCCATCCAGGAAGTCAAATCTGAAGCAGCGTTCCCTTCAGATGAAGCCATGCAGAATTCTGAGAACGGGGTGTCAACTAATCATCATGAGAAAGAGACGTTAGCTACCATCCAGGAAATCAAATCTGAAGCAGCATTGCCTTCAGATGAAGCGATGCAGAATTCTGAGAACGGGGTGTCAACTAATCATCATGAGAAAGAGACATTAGCTGCCATCCAGGAAATCAAATCTGAAGCAGCATTCCCTTCAGATGAAGCCATGACAAATTCTGAAAACGGAGTCTCAACTAATCATCATGAGAAAGAGACATTAGCTGCAGTCCAGGAAATCAAATCTGAAGCAGCATTCCGTTCAGAAGCCATGACGAATTCTGAGAACAGAGTCTCAACTAATCATCATGAGAAAGAGACATTAGCTGGCATCCAGGAAATCAAATCTGAAGCAGCATTCCTTTCAGAAGTCATGAAGAATTCTGGGAGCGATATATCAACTAATCTTCATGAGAAAGAGATGTACCAGTCATTTGTTTTGAATAATCAAGAGAATTTTGAAAGTGGGAAGTTAACAGAATTGCAATCCAAAGAGATCAATATTGGGAATGTTGTCACAGAAGTGTCATTTGCTCCCGAGGGATACATATTGGCGATGAGCGTGGAGATGAACAAACAGAATTGCAATGGAGATATGGTCTCTGGCGAATGA

Protein sequence

>Msa0747650

MSTTNEDFQVVGVSNCYVFKSQLQEYAQKAGLGTPVYETTKEGPSHEPSFRSTVIMNDVRYDSLTGFFNRKAAEQSAAEVALMELAKTGEVNQSITQPVHETGLCKNLLQEYAQKMNYAMPLYQSKKDDTPPGRAPLYSCTVDIGGMLYIGGTAKTKREAEIKAARTALLAIQTNASQASENQFGHLTVIPSRKRATESIADEASKAPKSKKSRFKGKYSKKKPHRNKKRRINTDNAGDGAKINNGEESLASANDESGLQEIKSEAAFPSEVMKNSENGVSSNHHEKETLAGLQEIKSEAAFPSEAMQYYDNGVSTNRHEKETLAGLQEVKSGAAFPSEAMKNSENGASTNYHEKETLAGLQEVKSEAAFPPDEAMQNSENGVSTNHHEKETLAAIKEVKSEAAFPSDEAMQNSENGASTNYHEKETLAAIQEVKSEAAFPSDEAMQNSENGVSTNHHEKETLATIQEIKSEAALPSDEAMQNSENGVSTNHHEKETLAAIQEIKSEAAFPSDEAMTNSENGVSTNHHEKETLAAVQEIKSEAAFRSEAMTNSENRVSTNHHEKETLAGIQEIKSEAAFLSEVMKNSGSDISTNLHEKEMYQSFVLNNQENFESGKLTELQSKEINIGNVVTEVSFAPEGYILAMSVEMNKQNCNGDMVSGE*