AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843360 MtrunA17_Chr5g0414061 95.205 438 17 2 1 438 12 445 0.0 874
Msa0843360 MtrunA17_Chr5g0414041 68.989 445 132 5 1 442 3 444 0.0 599
Msa0843360 MtrunA17_Chr5g0414091 63.409 440 149 6 3 435 1 435 0.0 547
Msa0843360 MtrunA17_Chr5g0414051 60.386 414 140 7 36 442 37 433 3.48e-177 502
Msa0843360 MtrunA17_Chr3g0140371 61.749 366 129 5 77 438 9 367 8.02e-170 481
Msa0843360 MtrunA17_Chr8g0391481 58.400 375 143 7 66 432 4 373 1.02e-158 453
Msa0843360 MtrunA17_Chr7g0226991 53.518 398 174 7 41 433 13 404 1.54e-152 439
Msa0843360 MtrunA17_Chr8g0347321 38.322 441 248 11 6 431 8 439 5.34e-102 311
Msa0843360 MtrunA17_Chr6g0456691 41.644 365 193 10 86 442 78 430 9.99e-100 305
Msa0843360 MtrunA17_Chr5g0414081 67.662 201 60 3 237 433 2 201 5.75e-98 292
Msa0843360 MtrunA17_Chr8g0387921 39.416 411 232 10 28 431 19 419 2.72e-97 298
Msa0843360 MtrunA17_Chr6g0451321 34.858 459 252 12 13 439 22 465 4.32e-89 278
Msa0843360 MtrunA17_Chr6g0456681 37.415 441 235 15 1 432 1 409 2.40e-86 270
Msa0843360 MtrunA17_Chr2g0283851 38.764 356 192 12 91 433 60 402 3.95e-79 251
Msa0843360 MtrunA17_Chr1g0188191 35.732 403 224 13 51 433 196 583 1.85e-70 233
Msa0843360 MtrunA17_Chr6g0476041 33.333 414 237 13 40 433 122 516 4.45e-67 223
Msa0843360 MtrunA17_Chr7g0221281 38.525 244 136 7 195 432 2 237 3.04e-55 184
Msa0843360 MtrunA17_Chr7g0259711 32.065 368 210 17 84 433 89 434 6.83e-49 172
Msa0843360 MtrunA17_Chr2g0287901 33.693 371 203 15 84 435 95 441 4.72e-48 171
Msa0843360 MtrunA17_Chr8g0384151 30.457 394 227 14 62 441 66 426 4.51e-44 159
Msa0843360 MtrunA17_Chr1g0175671 32.791 369 203 17 85 433 169 512 5.45e-44 161
Msa0843360 MtrunA17_Chr4g0070341 31.144 411 220 16 45 431 100 471 1.30e-43 159
Msa0843360 MtrunA17_Chr7g0258851 31.347 386 224 15 59 427 222 583 1.19e-42 158
Msa0843360 MtrunA17_Chr4g0071391 32.418 364 204 15 85 431 108 446 9.54e-42 154
Msa0843360 MtrunA17_Chr5g0430371 30.137 365 225 12 76 427 48 395 1.54e-41 152
Msa0843360 MtrunA17_Chr4g0068111 27.465 426 250 18 29 431 64 453 5.33e-41 152
Msa0843360 MtrunA17_Chr8g0384421 28.293 410 222 15 60 431 97 472 3.09e-40 150
Msa0843360 MtrunA17_Chr3g0105301 31.476 359 206 15 83 427 40 372 7.87e-40 147
Msa0843360 MtrunA17_Chr4g0005491 30.518 367 217 14 84 431 60 407 2.04e-39 146
Msa0843360 MtrunA17_Chr5g0413271 29.155 367 224 14 78 433 137 478 1.01e-38 146
Msa0843360 MtrunA17_Chr8g0368461 29.474 380 212 17 84 432 176 530 1.51e-38 146
Msa0843360 MtrunA17_Chr7g0221271 36.410 195 109 6 28 214 18 205 2.01e-38 138
Msa0843360 MtrunA17_Chr3g0126021 29.195 435 254 17 21 436 65 464 7.40e-38 143
Msa0843360 MtrunA17_Chr1g0213281 29.737 380 222 16 83 440 172 528 8.94e-38 144
Msa0843360 MtrunA17_Chr7g0269991 30.966 352 210 15 86 428 137 464 2.12e-37 142
Msa0843360 MtrunA17_Chr4g0006991 29.609 358 226 12 85 431 85 427 3.39e-37 140
Msa0843360 MtrunA17_Chr7g0243301 29.700 367 220 15 84 431 62 409 4.67e-37 140
Msa0843360 MtrunA17_Chr7g0242701 30.362 359 222 14 85 431 85 427 8.41e-37 139
Msa0843360 MtrunA17_Chr2g0325481 30.682 352 209 15 79 427 43 362 1.03e-36 138
Msa0843360 MtrunA17_Chr4g0045951 30.398 352 209 13 77 424 36 355 3.63e-36 136
Msa0843360 MtrunA17_Chr4g0045941 30.925 346 203 13 83 424 30 343 3.69e-36 136
Msa0843360 MtrunA17_Chr3g0085071 29.888 358 205 15 85 433 45 365 1.31e-35 135
Msa0843360 MtrunA17_Chr3g0113671 28.997 369 212 16 83 427 103 445 6.71e-35 135
Msa0843360 MtrunA17_Chr3g0091031 29.661 354 205 12 86 431 31 348 7.87e-35 132
Msa0843360 MtrunA17_Chr8g0360521 26.703 367 224 10 86 433 71 411 1.36e-34 133
Msa0843360 MtrunA17_Chr1g0171171 29.032 372 225 15 68 431 35 375 1.40e-34 132
Msa0843360 MtrunA17_Chr2g0314801 30.028 363 218 14 76 431 20 353 1.60e-34 132
Msa0843360 MtrunA17_Chr3g0085081 30.000 370 197 15 79 431 24 348 3.75e-34 130
Msa0843360 MtrunA17_Chr2g0283581 30.748 361 217 13 73 431 29 358 3.32e-33 128
Msa0843360 MtrunA17_Chr3g0114101 27.494 451 269 18 5 428 8 427 3.97e-33 129
Msa0843360 MtrunA17_Chr2g0314791 29.014 355 207 12 81 427 24 341 4.61e-33 127
Msa0843360 MtrunA17_Chr3g0097891 28.653 349 215 9 83 427 28 346 7.23e-33 127
Msa0843360 MtrunA17_Chr1g0210771 26.702 382 231 15 59 427 33 378 1.16e-32 127
Msa0843360 MtrunA17_Chr5g0437601 27.809 356 227 13 86 428 83 421 1.38e-32 128
Msa0843360 MtrunA17_Chr6g0455901 27.838 370 221 13 83 429 68 414 3.57e-32 126
Msa0843360 MtrunA17_Chr4g0041011 27.818 417 242 18 20 431 1 363 2.31e-31 123
Msa0843360 MtrunA17_Chr7g0221291 36.585 164 90 5 17 176 15 168 7.15e-30 114
Msa0843360 MtrunA17_Chr4g0005481 26.772 381 228 16 73 431 95 446 1.64e-28 117
Msa0843360 MtrunA17_Chr5g0413851 28.493 365 214 15 73 427 28 355 2.33e-27 112
Msa0843360 MtrunA17_Chr5g0437291 26.882 372 226 15 86 433 165 514 5.50e-27 113
Msa0843360 MtrunA17_Chr4g0049421 25.532 376 219 14 86 431 48 392 9.42e-27 111
Msa0843360 MtrunA17_Chr1g0178081 32.645 242 136 10 27 257 10 235 3.36e-25 105
Msa0843360 MtrunA17_Chr1g0178081 36.283 113 60 4 322 433 243 344 7.57e-13 69.7
Msa0843360 MtrunA17_Chr1g0209921 32.086 187 114 5 82 266 40 215 2.12e-22 96.3
Msa0843360 MtrunA17_Chr4g0057171 51.087 92 38 3 228 319 1 85 4.98e-22 91.3
Msa0843360 MtrunA17_Chr7g0275501 48.611 72 29 2 359 430 5 68 3.03e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843360 AT1G70230.1 52.093 430 186 9 9 435 3 415 9.17e-159 455
Msa0843360 AT4G01080.1 41.775 383 207 11 60 437 65 436 7.49e-98 300
Msa0843360 AT1G01430.1 39.018 387 221 8 62 439 75 455 6.51e-97 298
Msa0843360 AT5G15890.1 40.470 383 213 9 53 432 153 523 1.06e-95 298
Msa0843360 AT4G23790.1 37.557 442 250 11 1 431 1 427 1.93e-95 294
Msa0843360 AT4G11090.1 39.110 427 227 11 20 431 17 425 4.13e-95 293
Msa0843360 AT5G15900.1 37.071 437 223 15 18 437 14 415 2.66e-92 286
Msa0843360 AT5G15900.2 37.071 437 223 15 18 437 33 434 5.83e-92 285
Msa0843360 AT3G02440.1 39.377 353 198 8 86 432 127 469 6.17e-90 281
Msa0843360 AT3G02440.2 39.377 353 198 8 86 432 135 477 6.85e-90 281
Msa0843360 AT3G28150.1 35.955 445 240 15 1 433 1 412 2.59e-89 278
Msa0843360 AT4G25360.1 34.739 403 237 12 46 433 135 526 2.39e-69 229
Msa0843360 AT4G25360.2 34.739 403 237 12 46 433 135 526 2.39e-69 229
Msa0843360 AT5G51640.1 34.341 364 218 10 83 433 135 490 5.60e-65 217
Msa0843360 AT5G19160.1 32.973 370 224 11 84 440 97 455 1.13e-49 176
Msa0843360 AT3G06080.2 34.225 374 208 17 86 442 103 455 1.28e-49 176
Msa0843360 AT3G12060.1 32.222 360 202 15 86 427 196 531 3.24e-46 168
Msa0843360 AT5G64020.1 31.267 371 211 13 86 431 57 408 3.70e-46 165
Msa0843360 AT2G30010.1 32.520 369 204 14 83 431 53 396 1.23e-45 163
Msa0843360 AT2G30010.2 32.520 369 204 14 83 431 96 439 1.95e-45 164
Msa0843360 AT1G48880.1 33.058 363 184 14 85 428 114 436 8.20e-45 162
Msa0843360 AT5G06700.1 32.782 363 209 18 86 433 255 597 3.69e-43 160
Msa0843360 AT2G14530.1 29.517 393 228 13 62 431 40 406 3.87e-42 154
Msa0843360 AT5G06230.2 32.620 374 206 17 76 427 11 360 7.11e-42 152
Msa0843360 AT5G06230.1 32.620 374 206 17 76 427 52 401 1.54e-41 152
Msa0843360 AT5G20590.1 32.597 362 197 15 86 427 141 475 1.88e-41 154
Msa0843360 AT3G11570.1 33.065 372 200 17 78 427 71 415 3.43e-41 152
Msa0843360 AT2G37720.1 29.670 364 208 12 86 428 143 479 3.50e-41 153
Msa0843360 AT5G58600.1 29.444 360 221 11 83 431 63 400 4.81e-41 151
Msa0843360 AT3G62390.1 28.747 407 230 17 51 433 104 474 1.50e-39 148
Msa0843360 AT3G14850.2 29.395 347 210 11 86 427 34 350 4.20e-38 142
Msa0843360 AT5G49340.1 28.650 363 221 12 86 431 100 441 5.06e-38 144
Msa0843360 AT5G20680.6 28.325 406 244 13 44 431 81 457 4.06e-37 141
Msa0843360 AT2G38320.1 27.649 387 232 15 69 431 42 404 5.14e-37 140
Msa0843360 AT5G20680.4 28.325 406 244 13 44 431 175 551 1.15e-36 141
Msa0843360 AT5G20680.1 28.325 406 244 13 44 431 175 551 1.15e-36 141
Msa0843360 AT5G20680.3 28.325 406 244 13 44 431 175 551 1.15e-36 141
Msa0843360 AT5G20680.5 28.325 406 244 13 44 431 175 551 1.15e-36 141
Msa0843360 AT5G20680.2 28.325 406 244 13 44 431 157 533 1.17e-36 141
Msa0843360 AT1G60790.1 28.646 384 214 16 72 427 177 528 1.46e-36 141
Msa0843360 AT2G31110.2 30.114 352 200 17 85 427 44 358 4.77e-36 136
Msa0843360 AT3G11030.1 27.978 361 221 12 86 428 106 445 8.79e-36 137
Msa0843360 AT2G40160.1 27.335 439 252 19 28 433 22 426 1.65e-35 136
Msa0843360 AT2G40160.2 26.852 432 250 18 28 427 22 419 2.34e-35 136
Msa0843360 AT2G42570.1 28.871 381 225 17 60 431 21 364 2.64e-35 134
Msa0843360 AT5G01360.2 27.747 364 220 15 85 428 35 375 6.87e-35 134
Msa0843360 AT2G30900.1 29.070 344 217 9 86 427 43 361 1.03e-34 133
Msa0843360 AT2G34070.1 27.487 382 242 13 55 431 32 383 1.12e-34 133
Msa0843360 AT1G73140.1 28.571 371 216 16 78 427 59 401 2.08e-34 133
Msa0843360 AT5G01360.1 28.065 367 215 16 85 428 91 431 2.18e-34 133
Msa0843360 AT2G40320.1 27.919 394 219 19 70 428 56 419 9.60e-34 131
Msa0843360 AT1G29050.1 28.093 388 219 15 60 431 35 378 1.27e-33 130
Msa0843360 AT5G64470.3 29.810 369 207 16 86 431 55 394 3.49e-33 129
Msa0843360 AT5G19160.2 34.818 247 143 8 84 320 97 335 3.99e-33 128
Msa0843360 AT2G38320.2 27.907 344 202 13 111 431 1 321 1.25e-32 126
Msa0843360 AT3G55990.1 28.571 371 220 15 85 433 139 486 1.40e-32 129
Msa0843360 AT5G64470.2 29.333 375 207 17 86 431 55 400 1.05e-31 125
Msa0843360 AT2G40150.1 28.070 399 225 19 67 433 55 423 1.20e-31 125
Msa0843360 AT3G06080.1 35.081 248 139 10 86 321 103 340 1.84e-31 123
Msa0843360 AT1G78710.1 29.917 361 200 17 81 427 31 352 1.49e-30 121
Msa0843360 AT3G54260.1 27.824 363 206 17 86 431 51 374 3.39e-30 120
Msa0843360 AT5G58600.2 30.435 253 154 8 71 319 55 289 9.01e-29 115
Msa0843360 AT5G01620.3 25.613 367 231 14 83 431 115 457 1.11e-28 117
Msa0843360 AT5G01620.2 25.613 367 231 14 83 431 107 449 1.22e-28 117
Msa0843360 AT5G01620.1 25.613 367 231 14 83 431 107 449 1.22e-28 117
Msa0843360 AT3G62390.2 28.826 281 173 10 55 322 108 374 3.88e-28 115
Msa0843360 AT5G06230.3 34.016 244 133 12 76 300 52 286 6.32e-27 110
Msa0843360 AT5G20590.2 33.333 222 122 8 86 292 141 351 8.49e-27 111
Msa0843360 AT2G40160.3 29.123 285 161 10 28 292 22 285 2.85e-25 105
Msa0843360 AT2G31110.3 33.333 204 117 7 85 276 44 240 8.78e-25 103
Msa0843360 AT2G30900.2 32.065 184 118 4 86 268 43 220 1.45e-22 97.4
Msa0843360 AT1G78710.2 37.956 137 78 4 81 214 24 156 7.14e-19 87.0
Msa0843360 AT5G64470.1 28.159 277 164 13 86 342 55 316 2.80e-17 82.8
Msa0843360 AT5G64470.4 28.889 270 157 13 86 335 55 309 4.73e-17 82.0
Msa0843360 AT3G14850.1 25.455 275 171 10 158 427 2 247 3.15e-16 78.6

Find 91 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTTAATTACACTGAAAT+AGG 0.136015 5_4:-21009866 Msa0843360:CDS
TTCAAATATAGCTCATTATT+TGG 0.139372 5_4:+21010016 None:intergenic
TCCTATCAATTATGCTATTA+AGG 0.141791 5_4:+21009807 None:intergenic
CCTCCTTCATCTTCTTATTC+AGG 0.166411 5_4:-21010860 Msa0843360:intron
ATGTATTAAGAAAGGCTTTA+AGG 0.169604 5_4:-21009835 Msa0843360:CDS
ATTGATTGTCCTTGCCATTT+TGG 0.188794 5_4:+21010131 None:intergenic
AGTATTGGATGTAACAAAAT+TGG 0.211311 5_4:-21009564 Msa0843360:CDS
TTTCTATAAGGCTTAGTCTT+TGG 0.240548 5_4:+21009695 None:intergenic
AATTGATAGGAGAGGGAATA+AGG 0.252392 5_4:-21009795 Msa0843360:CDS
TTCTTCTCTCCATTTCTATA+AGG 0.257035 5_4:+21009683 None:intergenic
AAAGCCAAAGGCAATGAATT+TGG 0.260071 5_4:-21009602 Msa0843360:CDS
CTTAGTTGGAGAAAAGTAAT+TGG 0.278320 5_4:+21010256 None:intergenic
CCTAAACTTGTGTACCAAAA+TGG 0.283772 5_4:-21010145 Msa0843360:CDS
TGGAGTAATCACATTGGTTT+TGG 0.299673 5_4:+21010440 None:intergenic
ATGATTAAGGACACTTTCTA+TGG 0.319480 5_4:+21011120 None:intergenic
TACAAAGAAGAGACTCTAAT+TGG 0.322908 5_4:+21010188 None:intergenic
TATAGAAGAAGCAGAGTTAT+GGG 0.330047 5_4:+21010980 None:intergenic
GTCTTAATTACACTGAAATA+GGG 0.334111 5_4:-21009865 Msa0843360:CDS
CTTTCCAAATTCATTGCCTT+TGG 0.341956 5_4:+21009598 None:intergenic
ATTCAACTATTTCCTTGTAT+TGG 0.358515 5_4:-21010081 Msa0843360:CDS
AATGGAAAAGGGTTCATATA+AGG 0.359801 5_4:+21009512 None:intergenic
TGTGTTTGTTCTTACTTAGT+TGG 0.369018 5_4:+21010242 None:intergenic
TGAATCATGTTGATGAGAAA+TGG 0.370491 5_4:-21009997 Msa0843360:CDS
TGTGTTCATTGGTGTTTGCC+TGG 0.372187 5_4:-21009455 Msa0843360:CDS
AAGACTAAGCCTTATAGAAA+TGG 0.384572 5_4:-21009692 Msa0843360:CDS
GACATGATTGTACTATCAAT+TGG 0.399358 5_4:-21009953 Msa0843360:CDS
CCTATCAATTATGCTATTAA+GGG 0.403903 5_4:+21009808 None:intergenic
GTATAGAAGAAGCAGAGTTA+TGG 0.405691 5_4:+21010979 None:intergenic
AGTGATGTTGATGATGGGTT+TGG 0.406997 5_4:+21010907 None:intergenic
ACCCCATTAAAGAATGGAAA+AGG 0.407132 5_4:+21009500 None:intergenic
AAAGTAATTGGTTCAAACCT+TGG 0.414515 5_4:+21010268 None:intergenic
AAAGTTAAGATTTGAAGTAT+TGG 0.417492 5_4:-21009579 Msa0843360:CDS
TTGACTATCTTTATTGGAGA+TGG 0.423356 5_4:-21010312 Msa0843360:CDS
ATAGTACAATCATGTCCATT+TGG 0.433551 5_4:+21009960 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5_4:-21009466 Msa0843360:CDS
CAATAAAGATAGTCAAAATC+AGG 0.440358 5_4:+21010319 None:intergenic
ACACTTCATTCTTCACTCCT+AGG 0.443349 5_4:+21009374 None:intergenic
GTGATTACTCCAAGGGTGAT+TGG 0.451556 5_4:-21010429 Msa0843360:CDS
GAATCATGTTGATGAGAAAT+GGG 0.457430 5_4:-21009996 Msa0843360:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5_4:+21009530 None:intergenic
GGCAAAAGATATGCACCAAA+TGG 0.462882 5_4:-21009975 Msa0843360:CDS
AATGGAGAGAAGAAAGTTGA+AGG 0.470023 5_4:-21009674 Msa0843360:CDS
TTTAGAAATTATAAAGAAGT+GGG 0.470803 5_4:-21009405 Msa0843360:CDS
ATCTCATTCCATGTATCAAT+CGG 0.477028 5_4:+21009431 None:intergenic
TTGTTGGTGATTCTCTAGCT+AGG 0.478957 5_4:-21010213 Msa0843360:CDS
GTCTTTCACCCAATCACCCT+TGG 0.479066 5_4:+21010420 None:intergenic
TTAAGACCAGATGGTCATCC+AGG 0.486939 5_4:-21009536 Msa0843360:CDS
ATTGATAGGAGAGGGAATAA+GGG 0.492867 5_4:-21009794 Msa0843360:CDS
AGAGAAGAAAGTTGAAGGAA+TGG 0.493088 5_4:-21009669 Msa0843360:CDS
ATGTTGTATCATTGTACAAA+GGG 0.502439 5_4:+21010389 None:intergenic
CCCTTAATAGCATAATTGAT+AGG 0.509822 5_4:-21009808 Msa0843360:CDS
TGATTACTCCAAGGGTGATT+GGG 0.515569 5_4:-21010428 Msa0843360:CDS
TCTTGAACCCCATTAAAGAA+TGG 0.524242 5_4:+21009494 None:intergenic
ATGAGAAGTGATGTTGATGA+TGG 0.527795 5_4:+21010901 None:intergenic
AGTCAACGGTATAAGTCTCT+TGG 0.528315 5_4:+21011018 None:intergenic
GAAGGTGCTTGGGATAAGGC+TGG 0.529140 5_4:-21009725 Msa0843360:CDS
TGCCTGGACCGATTGATACA+TGG 0.530575 5_4:-21009439 Msa0843360:CDS
GAAAATTGAGATAGAAGAAG+TGG 0.532759 5_4:-21009633 Msa0843360:CDS
CAAATGAATGCAAAATTCCA+AGG 0.537827 5_4:-21010285 Msa0843360:CDS
CTTCCGGCTATTTACCATGA+GGG 0.538180 5_4:-21009917 Msa0843360:CDS
TTGGCTTTGTTAAGACCAGA+TGG 0.544671 5_4:-21009545 Msa0843360:CDS
CCCCATTAAAGAATGGAAAA+GGG 0.551208 5_4:+21009501 None:intergenic
ATACCTGAATAAGAAGATGA+AGG 0.553644 5_4:+21010857 None:intergenic
AGAAATTATAAAGAAGTGGG+AGG 0.558622 5_4:-21009402 Msa0843360:CDS
TTCCATGTATCAATCGGTCC+AGG 0.567285 5_4:+21009437 None:intergenic
CTAAAATTGAATCACCCTCA+TGG 0.567926 5_4:+21009903 None:intergenic
GGAAGAAATTGCATGACACA+TGG 0.573080 5_4:-21010346 Msa0843360:CDS
TCACCCTCATGGTAAATAGC+CGG 0.573329 5_4:+21009914 None:intergenic
TTGTACTATCAATTGGACAT+TGG 0.581516 5_4:-21009946 Msa0843360:CDS
CACCCTTGGAGTAATCACAT+TGG 0.595685 5_4:+21010434 None:intergenic
ATAGCATAATTGATAGGAGA+GGG 0.596014 5_4:-21009802 Msa0843360:CDS
AACCAATGTGATTACTCCAA+GGG 0.597083 5_4:-21010436 Msa0843360:CDS
AAACCAATGTGATTACTCCA+AGG 0.602264 5_4:-21010437 Msa0843360:CDS
CATGTTGTATCATTGTACAA+AGG 0.616088 5_4:+21010388 None:intergenic
GAAGAAATTGCATGACACAT+GGG 0.618181 5_4:-21010345 Msa0843360:CDS
TGAGAAGTGATGTTGATGAT+GGG 0.620902 5_4:+21010902 None:intergenic
AGCAAAGAACAGATAGTCAA+CGG 0.622586 5_4:+21011004 None:intergenic
AAAGGGTTCATATAAGGACC+TGG 0.626122 5_4:+21009518 None:intergenic
AATTGAGATAGAAGAAGTGG+TGG 0.634610 5_4:-21009630 Msa0843360:CDS
GCTTCCGGCTATTTACCATG+AGG 0.639421 5_4:-21009918 Msa0843360:CDS
GTGGTGGCTGCTAAAGCCAA+AGG 0.645087 5_4:-21009614 Msa0843360:CDS
AAGGAATGGATAGTGAGATG+AGG 0.645343 5_4:-21009655 Msa0843360:CDS
CAAGCACCTTCAAAATGATG+TGG 0.645352 5_4:+21009737 None:intergenic
TGTGGTGGCATGATGAAAGA+AGG 0.645471 5_4:-21010367 Msa0843360:CDS
TACAATGATACAACATGTGG+TGG 0.653616 5_4:-21010382 Msa0843360:CDS
ACTTGTGTACCAAAATGGCA+AGG 0.655571 5_4:-21010140 Msa0843360:CDS
CCTGAATAAGAAGATGAAGG+AGG 0.656132 5_4:+21010860 None:intergenic
AATAGCATAATTGATAGGAG+AGG 0.660993 5_4:-21009803 Msa0843360:CDS
AGAAGTGGGAGGAACAACCT+AGG 0.672972 5_4:-21009391 Msa0843360:CDS
GATTGGGTGAAAGACACTAG+AGG 0.717181 5_4:-21010412 Msa0843360:CDS
TTGTACAATGATACAACATG+TGG 0.723255 5_4:-21010385 Msa0843360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTCCTTAATTAGTT+TGG - chr5_4:21009887-21009906 Msa0843360:CDS 15.0%
!!! TTTTAGAAATTATAAAGAAG+TGG - chr5_4:21011144-21011163 Msa0843360:five_prime_UTR 15.0%
!! TTTAGAAATTATAAAGAAGT+GGG - chr5_4:21011145-21011164 Msa0843360:five_prime_UTR 15.0%
!!! ATTTTTTTCCTTAATTAGTT+TGG - chr5_4:21009887-21009906 Msa0843360:CDS 15.0%
!!! TTTTAGAAATTATAAAGAAG+TGG - chr5_4:21011144-21011163 Msa0843360:five_prime_UTR 15.0%
!! TTTAGAAATTATAAAGAAGT+GGG - chr5_4:21011145-21011164 Msa0843360:five_prime_UTR 15.0%
!!! TTAGTTTTGATCAATGATTA+AGG + chr5_4:21009446-21009465 None:intergenic 20.0%
!!! GAATTAGTAATCTCAATTTT+TGG + chr5_4:21009624-21009643 None:intergenic 20.0%
!!! AGTAATTTTATCGATTAAGA+TGG + chr5_4:21009931-21009950 None:intergenic 20.0%
!! TTCAAATATAGCTCATTATT+TGG + chr5_4:21010537-21010556 None:intergenic 20.0%
!!! GTTTTATGATGTATTAAGAA+AGG - chr5_4:21010707-21010726 Msa0843360:intron 20.0%
!!! AAAGTTAAGATTTGAAGTAT+TGG - chr5_4:21010971-21010990 Msa0843360:CDS 20.0%
!!! TTAGTTTTGATCAATGATTA+AGG + chr5_4:21009446-21009465 None:intergenic 20.0%
!!! GAATTAGTAATCTCAATTTT+TGG + chr5_4:21009624-21009643 None:intergenic 20.0%
!!! AGTAATTTTATCGATTAAGA+TGG + chr5_4:21009931-21009950 None:intergenic 20.0%
!! TTCAAATATAGCTCATTATT+TGG + chr5_4:21010537-21010556 None:intergenic 20.0%
!!! GTTTTATGATGTATTAAGAA+AGG - chr5_4:21010707-21010726 Msa0843360:intron 20.0%
!!! AAAGTTAAGATTTGAAGTAT+TGG - chr5_4:21010971-21010990 Msa0843360:CDS 20.0%
! ACAAAAAGAAGCTATGAAAA+TGG - chr5_4:21009470-21009489 Msa0843360:CDS 25.0%
! CAAAAAGAAGCTATGAAAAT+GGG - chr5_4:21009471-21009490 Msa0843360:CDS 25.0%
!!! ATTTTTGGAGTTTGAGAAAA+AGG + chr5_4:21009609-21009628 None:intergenic 25.0%
!!! ATGATGATTGTTTGTTTTGT+TGG - chr5_4:21010043-21010062 Msa0843360:CDS 25.0%
! ATGTTGTATCATTGTACAAA+GGG + chr5_4:21010164-21010183 None:intergenic 25.0%
! CAATAAAGATAGTCAAAATC+AGG + chr5_4:21010234-21010253 None:intergenic 25.0%
!!! CTGATTTTGACTATCTTTAT+TGG - chr5_4:21010232-21010251 Msa0843360:CDS 25.0%
! ATTCAACTATTTCCTTGTAT+TGG - chr5_4:21010469-21010488 Msa0843360:intron 25.0%
! TCAATACCTTGTACTAAAAA+TGG + chr5_4:21010498-21010517 None:intergenic 25.0%
! GTCTTAATTACACTGAAATA+GGG - chr5_4:21010685-21010704 Msa0843360:intron 25.0%
! ATGTATTAAGAAAGGCTTTA+AGG - chr5_4:21010715-21010734 Msa0843360:intron 25.0%
! CCTATCAATTATGCTATTAA+GGG + chr5_4:21010745-21010764 None:intergenic 25.0%
! TCCTATCAATTATGCTATTA+AGG + chr5_4:21010746-21010765 None:intergenic 25.0%
!! AGTATTGGATGTAACAAAAT+TGG - chr5_4:21010986-21011005 Msa0843360:CDS 25.0%
! ACAAAAAGAAGCTATGAAAA+TGG - chr5_4:21009470-21009489 Msa0843360:CDS 25.0%
! CAAAAAGAAGCTATGAAAAT+GGG - chr5_4:21009471-21009490 Msa0843360:CDS 25.0%
!!! ATTTTTGGAGTTTGAGAAAA+AGG + chr5_4:21009609-21009628 None:intergenic 25.0%
!!! ATGATGATTGTTTGTTTTGT+TGG - chr5_4:21010043-21010062 Msa0843360:CDS 25.0%
! ATGTTGTATCATTGTACAAA+GGG + chr5_4:21010164-21010183 None:intergenic 25.0%
! CAATAAAGATAGTCAAAATC+AGG + chr5_4:21010234-21010253 None:intergenic 25.0%
!!! CTGATTTTGACTATCTTTAT+TGG - chr5_4:21010232-21010251 Msa0843360:CDS 25.0%
! ATTCAACTATTTCCTTGTAT+TGG - chr5_4:21010469-21010488 Msa0843360:intron 25.0%
! TCAATACCTTGTACTAAAAA+TGG + chr5_4:21010498-21010517 None:intergenic 25.0%
! GTCTTAATTACACTGAAATA+GGG - chr5_4:21010685-21010704 Msa0843360:intron 25.0%
! ATGTATTAAGAAAGGCTTTA+AGG - chr5_4:21010715-21010734 Msa0843360:intron 25.0%
! CCTATCAATTATGCTATTAA+GGG + chr5_4:21010745-21010764 None:intergenic 25.0%
! TCCTATCAATTATGCTATTA+AGG + chr5_4:21010746-21010765 None:intergenic 25.0%
!! AGTATTGGATGTAACAAAAT+TGG - chr5_4:21010986-21011005 Msa0843360:CDS 25.0%
!!! TTTGTTGGGTTTTTTGCATT+AGG + chr5_4:21009407-21009426 None:intergenic 30.0%
!! ACTTTCTATGGTGTTTTGTT+GGG + chr5_4:21009421-21009440 None:intergenic 30.0%
ATGATTAAGGACACTTTCTA+TGG + chr5_4:21009433-21009452 None:intergenic 30.0%
TATAGAAGAAGCAGAGTTAT+GGG + chr5_4:21009573-21009592 None:intergenic 30.0%
GAGTTTGAGAAAAAGGTAAA+TGG + chr5_4:21009602-21009621 None:intergenic 30.0%
ATACCTGAATAAGAAGATGA+AGG + chr5_4:21009696-21009715 None:intergenic 30.0%
TTCGAACACCAAACTAATTA+AGG + chr5_4:21009898-21009917 None:intergenic 30.0%
CATGTTGTATCATTGTACAA+AGG + chr5_4:21010165-21010184 None:intergenic 30.0%
TTGTACAATGATACAACATG+TGG - chr5_4:21010165-21010184 Msa0843360:CDS 30.0%
TTGACTATCTTTATTGGAGA+TGG - chr5_4:21010238-21010257 Msa0843360:CDS 30.0%
! CTTGGAATTTTGCATTCATT+TGG + chr5_4:21010267-21010286 None:intergenic 30.0%
CAAATGAATGCAAAATTCCA+AGG - chr5_4:21010265-21010284 Msa0843360:CDS 30.0%
! AAAGTAATTGGTTCAAACCT+TGG + chr5_4:21010285-21010304 None:intergenic 30.0%
CTTAGTTGGAGAAAAGTAAT+TGG + chr5_4:21010297-21010316 None:intergenic 30.0%
TGTGTTTGTTCTTACTTAGT+TGG + chr5_4:21010311-21010330 None:intergenic 30.0%
!! AACAAACACATTGCTTTTGT+TGG - chr5_4:21010321-21010340 Msa0843360:CDS 30.0%
TACAAAGAAGAGACTCTAAT+TGG + chr5_4:21010365-21010384 None:intergenic 30.0%
TAAAAATGGAGACCAATACA+AGG + chr5_4:21010484-21010503 None:intergenic 30.0%
! TGAATCATGTTGATGAGAAA+TGG - chr5_4:21010553-21010572 Msa0843360:intron 30.0%
! GAATCATGTTGATGAGAAAT+GGG - chr5_4:21010554-21010573 Msa0843360:intron 30.0%
ATAGTACAATCATGTCCATT+TGG + chr5_4:21010593-21010612 None:intergenic 30.0%
GACATGATTGTACTATCAAT+TGG - chr5_4:21010597-21010616 Msa0843360:intron 30.0%
TTGTACTATCAATTGGACAT+TGG - chr5_4:21010604-21010623 Msa0843360:intron 30.0%
! ATGAGGGTGATTCAATTTTA+GGG - chr5_4:21010649-21010668 Msa0843360:intron 30.0%
!!! ATTTTAGGGTGTCATTATTG+TGG - chr5_4:21010663-21010682 Msa0843360:intron 30.0%
GGTCTTAATTACACTGAAAT+AGG - chr5_4:21010684-21010703 Msa0843360:intron 30.0%
CCCTTAATAGCATAATTGAT+AGG - chr5_4:21010742-21010761 Msa0843360:intron 30.0%
AATAGCATAATTGATAGGAG+AGG - chr5_4:21010747-21010766 Msa0843360:intron 30.0%
ATAGCATAATTGATAGGAGA+GGG - chr5_4:21010748-21010767 Msa0843360:intron 30.0%
! TTTACACCACATCATTTTGA+AGG - chr5_4:21010807-21010826 Msa0843360:intron 30.0%
! TTTCTATAAGGCTTAGTCTT+TGG + chr5_4:21010858-21010877 None:intergenic 30.0%
! AAGACTAAGCCTTATAGAAA+TGG - chr5_4:21010858-21010877 Msa0843360:intron 30.0%
TTCTTCTCTCCATTTCTATA+AGG + chr5_4:21010870-21010889 None:intergenic 30.0%
GAAAATTGAGATAGAAGAAG+TGG - chr5_4:21010917-21010936 Msa0843360:CDS 30.0%
AATGGAAAAGGGTTCATATA+AGG + chr5_4:21011041-21011060 None:intergenic 30.0%
! AACCCTTTTCCATTCTTTAA+TGG - chr5_4:21011047-21011066 Msa0843360:CDS 30.0%
! ACCCTTTTCCATTCTTTAAT+GGG - chr5_4:21011048-21011067 Msa0843360:CDS 30.0%
TGCAAAATGATTGTGTTCAT+TGG - chr5_4:21011084-21011103 Msa0843360:exon 30.0%
ATCTCATTCCATGTATCAAT+CGG + chr5_4:21011122-21011141 None:intergenic 30.0%
AGAAATTATAAAGAAGTGGG+AGG - chr5_4:21011148-21011167 Msa0843360:five_prime_UTR 30.0%
!!! TTTGTTGGGTTTTTTGCATT+AGG + chr5_4:21009407-21009426 None:intergenic 30.0%
!! ACTTTCTATGGTGTTTTGTT+GGG + chr5_4:21009421-21009440 None:intergenic 30.0%
ATGATTAAGGACACTTTCTA+TGG + chr5_4:21009433-21009452 None:intergenic 30.0%
TATAGAAGAAGCAGAGTTAT+GGG + chr5_4:21009573-21009592 None:intergenic 30.0%
GAGTTTGAGAAAAAGGTAAA+TGG + chr5_4:21009602-21009621 None:intergenic 30.0%
ATACCTGAATAAGAAGATGA+AGG + chr5_4:21009696-21009715 None:intergenic 30.0%
TTCGAACACCAAACTAATTA+AGG + chr5_4:21009898-21009917 None:intergenic 30.0%
CATGTTGTATCATTGTACAA+AGG + chr5_4:21010165-21010184 None:intergenic 30.0%
TTGTACAATGATACAACATG+TGG - chr5_4:21010165-21010184 Msa0843360:CDS 30.0%
TTGACTATCTTTATTGGAGA+TGG - chr5_4:21010238-21010257 Msa0843360:CDS 30.0%
! CTTGGAATTTTGCATTCATT+TGG + chr5_4:21010267-21010286 None:intergenic 30.0%
CAAATGAATGCAAAATTCCA+AGG - chr5_4:21010265-21010284 Msa0843360:CDS 30.0%
! AAAGTAATTGGTTCAAACCT+TGG + chr5_4:21010285-21010304 None:intergenic 30.0%
CTTAGTTGGAGAAAAGTAAT+TGG + chr5_4:21010297-21010316 None:intergenic 30.0%
TGTGTTTGTTCTTACTTAGT+TGG + chr5_4:21010311-21010330 None:intergenic 30.0%
!! AACAAACACATTGCTTTTGT+TGG - chr5_4:21010321-21010340 Msa0843360:CDS 30.0%
TACAAAGAAGAGACTCTAAT+TGG + chr5_4:21010365-21010384 None:intergenic 30.0%
TAAAAATGGAGACCAATACA+AGG + chr5_4:21010484-21010503 None:intergenic 30.0%
! TGAATCATGTTGATGAGAAA+TGG - chr5_4:21010553-21010572 Msa0843360:intron 30.0%
! GAATCATGTTGATGAGAAAT+GGG - chr5_4:21010554-21010573 Msa0843360:intron 30.0%
ATAGTACAATCATGTCCATT+TGG + chr5_4:21010593-21010612 None:intergenic 30.0%
GACATGATTGTACTATCAAT+TGG - chr5_4:21010597-21010616 Msa0843360:intron 30.0%
TTGTACTATCAATTGGACAT+TGG - chr5_4:21010604-21010623 Msa0843360:intron 30.0%
! ATGAGGGTGATTCAATTTTA+GGG - chr5_4:21010649-21010668 Msa0843360:intron 30.0%
!!! ATTTTAGGGTGTCATTATTG+TGG - chr5_4:21010663-21010682 Msa0843360:intron 30.0%
GGTCTTAATTACACTGAAAT+AGG - chr5_4:21010684-21010703 Msa0843360:intron 30.0%
CCCTTAATAGCATAATTGAT+AGG - chr5_4:21010742-21010761 Msa0843360:intron 30.0%
AATAGCATAATTGATAGGAG+AGG - chr5_4:21010747-21010766 Msa0843360:intron 30.0%
ATAGCATAATTGATAGGAGA+GGG - chr5_4:21010748-21010767 Msa0843360:intron 30.0%
! TTTACACCACATCATTTTGA+AGG - chr5_4:21010807-21010826 Msa0843360:intron 30.0%
! TTTCTATAAGGCTTAGTCTT+TGG + chr5_4:21010858-21010877 None:intergenic 30.0%
! AAGACTAAGCCTTATAGAAA+TGG - chr5_4:21010858-21010877 Msa0843360:intron 30.0%
TTCTTCTCTCCATTTCTATA+AGG + chr5_4:21010870-21010889 None:intergenic 30.0%
GAAAATTGAGATAGAAGAAG+TGG - chr5_4:21010917-21010936 Msa0843360:CDS 30.0%
AATGGAAAAGGGTTCATATA+AGG + chr5_4:21011041-21011060 None:intergenic 30.0%
! AACCCTTTTCCATTCTTTAA+TGG - chr5_4:21011047-21011066 Msa0843360:CDS 30.0%
! ACCCTTTTCCATTCTTTAAT+GGG - chr5_4:21011048-21011067 Msa0843360:CDS 30.0%
TGCAAAATGATTGTGTTCAT+TGG - chr5_4:21011084-21011103 Msa0843360:exon 30.0%
ATCTCATTCCATGTATCAAT+CGG + chr5_4:21011122-21011141 None:intergenic 30.0%
AGAAATTATAAAGAAGTGGG+AGG - chr5_4:21011148-21011167 Msa0843360:five_prime_UTR 30.0%
! CACTTTCTATGGTGTTTTGT+TGG + chr5_4:21009422-21009441 None:intergenic 35.0%
AGCAAAGAACAGATAGTCAA+CGG + chr5_4:21009549-21009568 None:intergenic 35.0%
GTATAGAAGAAGCAGAGTTA+TGG + chr5_4:21009574-21009593 None:intergenic 35.0%
!! TGAGAAGTGATGTTGATGAT+GGG + chr5_4:21009651-21009670 None:intergenic 35.0%
! ATGAGAAGTGATGTTGATGA+TGG + chr5_4:21009652-21009671 None:intergenic 35.0%
!!! TGGAGTAATCACATTGGTTT+TGG + chr5_4:21010113-21010132 None:intergenic 35.0%
AAACCAATGTGATTACTCCA+AGG - chr5_4:21010113-21010132 Msa0843360:CDS 35.0%
AACCAATGTGATTACTCCAA+GGG - chr5_4:21010114-21010133 Msa0843360:CDS 35.0%
TACAATGATACAACATGTGG+TGG - chr5_4:21010168-21010187 Msa0843360:CDS 35.0%
GAAGAAATTGCATGACACAT+GGG - chr5_4:21010205-21010224 Msa0843360:CDS 35.0%
!! CCATTTTGGTACACAAGTTT+AGG + chr5_4:21010408-21010427 None:intergenic 35.0%
CCTAAACTTGTGTACCAAAA+TGG - chr5_4:21010405-21010424 Msa0843360:CDS 35.0%
!! ATTGATTGTCCTTGCCATTT+TGG + chr5_4:21010422-21010441 None:intergenic 35.0%
! GCAAGGACAATCAATTTTCT+AGG - chr5_4:21010427-21010446 Msa0843360:CDS 35.0%
! AGGACAATCAATTTTCTAGG+TGG - chr5_4:21010430-21010449 Msa0843360:CDS 35.0%
!!! TGGTCTCCATTTTTAGTACA+AGG - chr5_4:21010489-21010508 Msa0843360:intron 35.0%
CTAAAATTGAATCACCCTCA+TGG + chr5_4:21010650-21010669 None:intergenic 35.0%
! CATGAGGGTGATTCAATTTT+AGG - chr5_4:21010648-21010667 Msa0843360:intron 35.0%
AATTGATAGGAGAGGGAATA+AGG - chr5_4:21010755-21010774 Msa0843360:intron 35.0%
ATTGATAGGAGAGGGAATAA+GGG - chr5_4:21010756-21010775 Msa0843360:intron 35.0%
!!! ACATCATTTTGAAGGTGCTT+GGG - chr5_4:21010815-21010834 Msa0843360:intron 35.0%
AATGGAGAGAAGAAAGTTGA+AGG - chr5_4:21010876-21010895 Msa0843360:CDS 35.0%
AGAGAAGAAAGTTGAAGGAA+TGG - chr5_4:21010881-21010900 Msa0843360:CDS 35.0%
AATTGAGATAGAAGAAGTGG+TGG - chr5_4:21010920-21010939 Msa0843360:CDS 35.0%
AAAGCCAAAGGCAATGAATT+TGG - chr5_4:21010948-21010967 Msa0843360:CDS 35.0%
CTTTCCAAATTCATTGCCTT+TGG + chr5_4:21010955-21010974 None:intergenic 35.0%
CCCCATTAAAGAATGGAAAA+GGG + chr5_4:21011052-21011071 None:intergenic 35.0%
! CCCTTTTCCATTCTTTAATG+GGG - chr5_4:21011049-21011068 Msa0843360:CDS 35.0%
ACCCCATTAAAGAATGGAAA+AGG + chr5_4:21011053-21011072 None:intergenic 35.0%
TCTTGAACCCCATTAAAGAA+TGG + chr5_4:21011059-21011078 None:intergenic 35.0%
! CACTTTCTATGGTGTTTTGT+TGG + chr5_4:21009422-21009441 None:intergenic 35.0%
AGCAAAGAACAGATAGTCAA+CGG + chr5_4:21009549-21009568 None:intergenic 35.0%
GTATAGAAGAAGCAGAGTTA+TGG + chr5_4:21009574-21009593 None:intergenic 35.0%
!! TGAGAAGTGATGTTGATGAT+GGG + chr5_4:21009651-21009670 None:intergenic 35.0%
! ATGAGAAGTGATGTTGATGA+TGG + chr5_4:21009652-21009671 None:intergenic 35.0%
!!! TGGAGTAATCACATTGGTTT+TGG + chr5_4:21010113-21010132 None:intergenic 35.0%
AAACCAATGTGATTACTCCA+AGG - chr5_4:21010113-21010132 Msa0843360:CDS 35.0%
AACCAATGTGATTACTCCAA+GGG - chr5_4:21010114-21010133 Msa0843360:CDS 35.0%
TACAATGATACAACATGTGG+TGG - chr5_4:21010168-21010187 Msa0843360:CDS 35.0%
GAAGAAATTGCATGACACAT+GGG - chr5_4:21010205-21010224 Msa0843360:CDS 35.0%
!! CCATTTTGGTACACAAGTTT+AGG + chr5_4:21010408-21010427 None:intergenic 35.0%
CCTAAACTTGTGTACCAAAA+TGG - chr5_4:21010405-21010424 Msa0843360:CDS 35.0%
!! ATTGATTGTCCTTGCCATTT+TGG + chr5_4:21010422-21010441 None:intergenic 35.0%
! GCAAGGACAATCAATTTTCT+AGG - chr5_4:21010427-21010446 Msa0843360:CDS 35.0%
! AGGACAATCAATTTTCTAGG+TGG - chr5_4:21010430-21010449 Msa0843360:CDS 35.0%
!!! TGGTCTCCATTTTTAGTACA+AGG - chr5_4:21010489-21010508 Msa0843360:intron 35.0%
CTAAAATTGAATCACCCTCA+TGG + chr5_4:21010650-21010669 None:intergenic 35.0%
! CATGAGGGTGATTCAATTTT+AGG - chr5_4:21010648-21010667 Msa0843360:intron 35.0%
AATTGATAGGAGAGGGAATA+AGG - chr5_4:21010755-21010774 Msa0843360:intron 35.0%
ATTGATAGGAGAGGGAATAA+GGG - chr5_4:21010756-21010775 Msa0843360:intron 35.0%
!!! ACATCATTTTGAAGGTGCTT+GGG - chr5_4:21010815-21010834 Msa0843360:intron 35.0%
AATGGAGAGAAGAAAGTTGA+AGG - chr5_4:21010876-21010895 Msa0843360:CDS 35.0%
AGAGAAGAAAGTTGAAGGAA+TGG - chr5_4:21010881-21010900 Msa0843360:CDS 35.0%
AATTGAGATAGAAGAAGTGG+TGG - chr5_4:21010920-21010939 Msa0843360:CDS 35.0%
AAAGCCAAAGGCAATGAATT+TGG - chr5_4:21010948-21010967 Msa0843360:CDS 35.0%
CTTTCCAAATTCATTGCCTT+TGG + chr5_4:21010955-21010974 None:intergenic 35.0%
CCCCATTAAAGAATGGAAAA+GGG + chr5_4:21011052-21011071 None:intergenic 35.0%
! CCCTTTTCCATTCTTTAATG+GGG - chr5_4:21011049-21011068 Msa0843360:CDS 35.0%
ACCCCATTAAAGAATGGAAA+AGG + chr5_4:21011053-21011072 None:intergenic 35.0%
TCTTGAACCCCATTAAAGAA+TGG + chr5_4:21011059-21011078 None:intergenic 35.0%
AGTCAACGGTATAAGTCTCT+TGG + chr5_4:21009535-21009554 None:intergenic 40.0%
!! AGTGATGTTGATGATGGGTT+TGG + chr5_4:21009646-21009665 None:intergenic 40.0%
CCTGAATAAGAAGATGAAGG+AGG + chr5_4:21009693-21009712 None:intergenic 40.0%
CCTCCTTCATCTTCTTATTC+AGG - chr5_4:21009690-21009709 Msa0843360:CDS 40.0%
TGATTACTCCAAGGGTGATT+GGG - chr5_4:21010122-21010141 Msa0843360:CDS 40.0%
GGAAGAAATTGCATGACACA+TGG - chr5_4:21010204-21010223 Msa0843360:CDS 40.0%
!! TTGTTGGTGATTCTCTAGCT+AGG - chr5_4:21010337-21010356 Msa0843360:CDS 40.0%
ACTTGTGTACCAAAATGGCA+AGG - chr5_4:21010410-21010429 Msa0843360:CDS 40.0%
GGCAAAAGATATGCACCAAA+TGG - chr5_4:21010575-21010594 Msa0843360:intron 40.0%
!!! TGGACATTGGTTTTTGCTTC+CGG - chr5_4:21010617-21010636 Msa0843360:intron 40.0%
CAAGCACCTTCAAAATGATG+TGG + chr5_4:21010816-21010835 None:intergenic 40.0%
!!! CACATCATTTTGAAGGTGCT+TGG - chr5_4:21010814-21010833 Msa0843360:intron 40.0%
!!! TTTTGAAGGTGCTTGGGATA+AGG - chr5_4:21010821-21010840 Msa0843360:intron 40.0%
AAGGAATGGATAGTGAGATG+AGG - chr5_4:21010895-21010914 Msa0843360:CDS 40.0%
!! TTGGCTTTGTTAAGACCAGA+TGG - chr5_4:21011005-21011024 Msa0843360:CDS 40.0%
AAAGGGTTCATATAAGGACC+TGG + chr5_4:21011035-21011054 None:intergenic 40.0%
AGTCAACGGTATAAGTCTCT+TGG + chr5_4:21009535-21009554 None:intergenic 40.0%
!! AGTGATGTTGATGATGGGTT+TGG + chr5_4:21009646-21009665 None:intergenic 40.0%
CCTGAATAAGAAGATGAAGG+AGG + chr5_4:21009693-21009712 None:intergenic 40.0%
CCTCCTTCATCTTCTTATTC+AGG - chr5_4:21009690-21009709 Msa0843360:CDS 40.0%
TGATTACTCCAAGGGTGATT+GGG - chr5_4:21010122-21010141 Msa0843360:CDS 40.0%
GGAAGAAATTGCATGACACA+TGG - chr5_4:21010204-21010223 Msa0843360:CDS 40.0%
!! TTGTTGGTGATTCTCTAGCT+AGG - chr5_4:21010337-21010356 Msa0843360:CDS 40.0%
ACTTGTGTACCAAAATGGCA+AGG - chr5_4:21010410-21010429 Msa0843360:CDS 40.0%
GGCAAAAGATATGCACCAAA+TGG - chr5_4:21010575-21010594 Msa0843360:intron 40.0%
!!! TGGACATTGGTTTTTGCTTC+CGG - chr5_4:21010617-21010636 Msa0843360:intron 40.0%
CAAGCACCTTCAAAATGATG+TGG + chr5_4:21010816-21010835 None:intergenic 40.0%
!!! CACATCATTTTGAAGGTGCT+TGG - chr5_4:21010814-21010833 Msa0843360:intron 40.0%
!!! TTTTGAAGGTGCTTGGGATA+AGG - chr5_4:21010821-21010840 Msa0843360:intron 40.0%
AAGGAATGGATAGTGAGATG+AGG - chr5_4:21010895-21010914 Msa0843360:CDS 40.0%
!! TTGGCTTTGTTAAGACCAGA+TGG - chr5_4:21011005-21011024 Msa0843360:CDS 40.0%
AAAGGGTTCATATAAGGACC+TGG + chr5_4:21011035-21011054 None:intergenic 40.0%
CACCCTTGGAGTAATCACAT+TGG + chr5_4:21010119-21010138 None:intergenic 45.0%
GTGATTACTCCAAGGGTGAT+TGG - chr5_4:21010121-21010140 Msa0843360:CDS 45.0%
GATTGGGTGAAAGACACTAG+AGG - chr5_4:21010138-21010157 Msa0843360:CDS 45.0%
TGTGGTGGCATGATGAAAGA+AGG - chr5_4:21010183-21010202 Msa0843360:CDS 45.0%
CTTCCGGCTATTTACCATGA+GGG - chr5_4:21010633-21010652 Msa0843360:intron 45.0%
TCACCCTCATGGTAAATAGC+CGG + chr5_4:21010639-21010658 None:intergenic 45.0%
TTAAGACCAGATGGTCATCC+AGG - chr5_4:21011014-21011033 Msa0843360:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - chr5_4:21011095-21011114 Msa0843360:five_prime_UTR 45.0%
TTCCATGTATCAATCGGTCC+AGG + chr5_4:21011116-21011135 None:intergenic 45.0%
CACCCTTGGAGTAATCACAT+TGG + chr5_4:21010119-21010138 None:intergenic 45.0%
GTGATTACTCCAAGGGTGAT+TGG - chr5_4:21010121-21010140 Msa0843360:CDS 45.0%
GATTGGGTGAAAGACACTAG+AGG - chr5_4:21010138-21010157 Msa0843360:CDS 45.0%
TGTGGTGGCATGATGAAAGA+AGG - chr5_4:21010183-21010202 Msa0843360:CDS 45.0%
CTTCCGGCTATTTACCATGA+GGG - chr5_4:21010633-21010652 Msa0843360:intron 45.0%
TCACCCTCATGGTAAATAGC+CGG + chr5_4:21010639-21010658 None:intergenic 45.0%
TTAAGACCAGATGGTCATCC+AGG - chr5_4:21011014-21011033 Msa0843360:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - chr5_4:21011095-21011114 Msa0843360:five_prime_UTR 45.0%
TTCCATGTATCAATCGGTCC+AGG + chr5_4:21011116-21011135 None:intergenic 45.0%
!! AAAATAATATATATTTAGAT+TGG + chr5_4:21009844-21009863 None:intergenic 5.0%
!! AAAATAATATATATTTAGAT+TGG + chr5_4:21009844-21009863 None:intergenic 5.0%
GTCTTTCACCCAATCACCCT+TGG + chr5_4:21010133-21010152 None:intergenic 50.0%
GCTTCCGGCTATTTACCATG+AGG - chr5_4:21010632-21010651 Msa0843360:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_4:21011023-21011042 None:intergenic 50.0%
TGCCTGGACCGATTGATACA+TGG - chr5_4:21011111-21011130 Msa0843360:five_prime_UTR 50.0%
AGAAGTGGGAGGAACAACCT+AGG - chr5_4:21011159-21011178 Msa0843360:five_prime_UTR 50.0%
GTCTTTCACCCAATCACCCT+TGG + chr5_4:21010133-21010152 None:intergenic 50.0%
GCTTCCGGCTATTTACCATG+AGG - chr5_4:21010632-21010651 Msa0843360:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_4:21011023-21011042 None:intergenic 50.0%
TGCCTGGACCGATTGATACA+TGG - chr5_4:21011111-21011130 Msa0843360:five_prime_UTR 50.0%
AGAAGTGGGAGGAACAACCT+AGG - chr5_4:21011159-21011178 Msa0843360:five_prime_UTR 50.0%
!! GAAGGTGCTTGGGATAAGGC+TGG - chr5_4:21010825-21010844 Msa0843360:intron 55.0%
GTGGTGGCTGCTAAAGCCAA+AGG - chr5_4:21010936-21010955 Msa0843360:CDS 55.0%
!! GAAGGTGCTTGGGATAAGGC+TGG - chr5_4:21010825-21010844 Msa0843360:intron 55.0%
GTGGTGGCTGCTAAAGCCAA+AGG - chr5_4:21010936-21010955 Msa0843360:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 21009379 21011193 21009379 ID=Msa0843360;Name=Msa0843360
chr5_4 mRNA 21009379 21011193 21009379 ID=Msa0843360-mRNA-1;Parent=Msa0843360;Name=Msa0843360-mRNA-1;_AED=0.01;_eAED=0.01;_QI=104|1|0.5|1|1|1|2|0|442
chr5_4 exon 21009379 21010478 21009379 ID=Msa0843360-mRNA-1:exon:8716;Parent=Msa0843360-mRNA-1
chr5_4 exon 21010861 21011193 21010861 ID=Msa0843360-mRNA-1:exon:8715;Parent=Msa0843360-mRNA-1
chr5_4 five_prime_UTR 21011090 21011193 21011090 ID=Msa0843360-mRNA-1:five_prime_utr;Parent=Msa0843360-mRNA-1
chr5_4 CDS 21010861 21011089 21010861 ID=Msa0843360-mRNA-1:cds;Parent=Msa0843360-mRNA-1
chr5_4 CDS 21009379 21010478 21009379 ID=Msa0843360-mRNA-1:cds;Parent=Msa0843360-mRNA-1
Gene Sequence

>Msa0843360

ATGAAAATGGGAACTTCAATATTTTTGAAAGATAAATCAAATTTATTCACCAAGAGACTTATACCGTTGACTATCTGTTCTTTGCTTCCCATAACTCTGCTTCTTCTATACTTCTACCATTTACCTTTTTCTCAAACTCCAAAAATTGAGATTACTAATTCCAAACCCATCATCAACATCACTTCTCATTCTTCTCTTTCTGCTTCTCCTCCTTCATCTTCTTATTCAGAAAAGAAAAAATCTTACCAAAACCAATGTGATTACTCCAAGGGTGATTGGGTGAAAGACACTAGAGGCCCTTTGTACAATGATACAACATGTGGTGGCATGATGAAAGAAGGAAGAAATTGCATGACACATGGGAGACCTGATTTTGACTATCTTTATTGGAGATGGAAACCAAATGAATGCAAAATTCCAAGGTTTGAACCAATTACTTTTCTCCAACTAAGTAAGAACAAACACATTGCTTTTGTTGGTGATTCTCTAGCTAGGAACCAATTAGAGTCTCTTCTTTGTATGTTAACAACTGCTTCAATTCCTAAACTTGTGTACCAAAATGGCAAGGACAATCAATTTTCTAGGTGGTACTTTTCTTCATACAATTCAACTATTTCCTTGTATTGGTCTCCATTTTTAGTACAAGGTATTGAAAAATCAAACACAAGTCCAAATAATGAGCTATATTTGAATCATGTTGATGAGAAATGGGCAAAAGATATGCACCAAATGGACATGATTGTACTATCAATTGGACATTGGTTTTTGCTTCCGGCTATTTACCATGAGGGTGATTCAATTTTAGGGTGTCATTATTGTGGTCTTAATTACACTGAAATAGGGTTTTATGATGTATTAAGAAAGGCTTTAAGGACTACCCTTAATAGCATAATTGATAGGAGAGGGAATAAGGGTAATGAAATTGATGTAGTTGTCACAACATTTACACCACATCATTTTGAAGGTGCTTGGGATAAGGCTGGTGCTTGTCCAAAGACTAAGCCTTATAGAAATGGAGAGAAGAAAGTTGAAGGAATGGATAGTGAGATGAGGAAAATTGAGATAGAAGAAGTGGTGGCTGCTAAAGCCAAAGGCAATGAATTTGGAAAGTTAAGATTTGAAGTATTGGATGTAACAAAATTGGCTTTGTTAAGACCAGATGGTCATCCAGGTCCTTATATGAACCCTTTTCCATTCTTTAATGGGGTTCAAGAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGACCGATTGATACATGGAATGAGATATTTTTAGAAATTATAAAGAAGTGGGAGGAACAACCTAGGAGTGAAGAATGA
Protein sequence

>Msa0843360

MKMGTSIFLKDKSNLFTKRLIPLTICSLLPITLLLLYFYHLPFSQTPKIEITNSKPIINITSHSSLSASPPSSSYSEKKKSYQNQCDYSKGDWVKDTRGPLYNDTTCGGMMKEGRNCMTHGRPDFDYLYWRWKPNECKIPRFEPITFLQLSKNKHIAFVGDSLARNQLESLLCMLTTASIPKLVYQNGKDNQFSRWYFSSYNSTISLYWSPFLVQGIEKSNTSPNNELYLNHVDEKWAKDMHQMDMIVLSIGHWFLLPAIYHEGDSILGCHYCGLNYTEIGFYDVLRKALRTTLNSIIDRRGNKGNEIDVVVTTFTPHHFEGAWDKAGACPKTKPYRNGEKKVEGMDSEMRKIEIEEVVAAKAKGNEFGKLRFEVLDVTKLALLRPDGHPGPYMNPFPFFNGVQEYVQNDCVHWCLPGPIDTWNEIFLEIIKKWEEQPRSEE*