AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1378040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1378040 MtrunA17_Chr8g0388181 96.032 126 5 0 1 126 1 126 2.16e-83 239
Msa1378040 MtrunA17_Chr8g0388191 75.397 126 28 3 1 125 5 128 1.13e-59 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCATGCAGTTTGCTTT+TGG 0.234778 tig0020047:+69233 None:intergenic
GGACTCAGAACCAACCATTT+TGG 0.266732 tig0020047:-69188 Msa1378040:CDS
CGTGAGTTACTTTCTATTCA+AGG 0.359033 tig0020047:-69001 Msa1378040:CDS
TAGTAATGATTTAGTAGAAA+AGG 0.375647 tig0020047:-69119 Msa1378040:CDS
AAAGAAAAGGGTCCGGTTTG+TGG 0.426219 tig0020047:-69209 Msa1378040:CDS
CTGCATGCAAGACAAAGAAA+AGG 0.503875 tig0020047:-69222 Msa1378040:CDS
TAATGATTTAGTAGAAAAGG+AGG 0.512981 tig0020047:-69116 Msa1378040:CDS
GTGAGTTACTTTCTATTCAA+GGG 0.520035 tig0020047:-69000 Msa1378040:CDS
TGGAAGTGTTCTTGAATTGA+AGG 0.537857 tig0020047:-69032 Msa1378040:CDS
AATGATTTAGTAGAAAAGGA+GGG 0.545782 tig0020047:-69115 Msa1378040:CDS
TGCATGCAAGACAAAGAAAA+GGG 0.550595 tig0020047:-69221 Msa1378040:CDS
TTGGTTCTGAGTCCACAAAC+CGG 0.560281 tig0020047:+69197 None:intergenic
GCAAGACAAAGAAAAGGGTC+CGG 0.569438 tig0020047:-69216 Msa1378040:CDS
TTTGTACAAATGCTCCCTCA+CGG 0.598040 tig0020047:+68975 None:intergenic
TCTATTCAAGGGAACCGTGA+GGG 0.615406 tig0020047:-68989 Msa1378040:CDS
GAGTTGCTATCAAGATGCAA+TGG 0.620407 tig0020047:-69052 Msa1378040:CDS
AGTGACGATTAGATTAACAA+AGG 0.646753 tig0020047:-69086 Msa1378040:CDS
TTCTATTCAAGGGAACCGTG+AGG 0.687443 tig0020047:-68990 Msa1378040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTGATATTTTTTTA+AGG + tig0020047:68975-68994 None:intergenic 10.0%
!!! TTTTTTGTGATATTTTTTTA+AGG + tig0020047:68975-68994 None:intergenic 10.0%
!! TAGTAATGATTTAGTAGAAA+AGG - tig0020047:69161-69180 Msa1378040:CDS 20.0%
!! TAGTAATGATTTAGTAGAAA+AGG - tig0020047:69161-69180 Msa1378040:CDS 20.0%
! TAATGATTTAGTAGAAAAGG+AGG - tig0020047:69164-69183 Msa1378040:CDS 25.0%
! AATGATTTAGTAGAAAAGGA+GGG - tig0020047:69165-69184 Msa1378040:CDS 25.0%
! TAATGATTTAGTAGAAAAGG+AGG - tig0020047:69164-69183 Msa1378040:CDS 25.0%
! AATGATTTAGTAGAAAAGGA+GGG - tig0020047:69165-69184 Msa1378040:CDS 25.0%
AGTGACGATTAGATTAACAA+AGG - tig0020047:69194-69213 Msa1378040:CDS 30.0%
! GTGAGTTACTTTCTATTCAA+GGG - tig0020047:69280-69299 Msa1378040:CDS 30.0%
AGTGACGATTAGATTAACAA+AGG - tig0020047:69194-69213 Msa1378040:CDS 30.0%
! GTGAGTTACTTTCTATTCAA+GGG - tig0020047:69280-69299 Msa1378040:CDS 30.0%
TGCATGCAAGACAAAGAAAA+GGG - tig0020047:69059-69078 Msa1378040:CDS 35.0%
! TTTTCTTCTCCCAAAATGGT+TGG + tig0020047:69105-69124 None:intergenic 35.0%
!! GTTGTTTTCTTCTCCCAAAA+TGG + tig0020047:69109-69128 None:intergenic 35.0%
!! TGGAAGTGTTCTTGAATTGA+AGG - tig0020047:69248-69267 Msa1378040:CDS 35.0%
! CGTGAGTTACTTTCTATTCA+AGG - tig0020047:69279-69298 Msa1378040:CDS 35.0%
TGCATGCAAGACAAAGAAAA+GGG - tig0020047:69059-69078 Msa1378040:CDS 35.0%
! TTTTCTTCTCCCAAAATGGT+TGG + tig0020047:69105-69124 None:intergenic 35.0%
!! GTTGTTTTCTTCTCCCAAAA+TGG + tig0020047:69109-69128 None:intergenic 35.0%
!! TGGAAGTGTTCTTGAATTGA+AGG - tig0020047:69248-69267 Msa1378040:CDS 35.0%
! CGTGAGTTACTTTCTATTCA+AGG - tig0020047:69279-69298 Msa1378040:CDS 35.0%
!! CTTGCATGCAGTTTGCTTTT+GGG + tig0020047:69049-69068 None:intergenic 40.0%
!! TCTTGCATGCAGTTTGCTTT+TGG + tig0020047:69050-69069 None:intergenic 40.0%
CTGCATGCAAGACAAAGAAA+AGG - tig0020047:69058-69077 Msa1378040:CDS 40.0%
! GACTCAGAACCAACCATTTT+GGG - tig0020047:69093-69112 Msa1378040:CDS 40.0%
GAGTTGCTATCAAGATGCAA+TGG - tig0020047:69228-69247 Msa1378040:CDS 40.0%
TTTGTACAAATGCTCCCTCA+CGG + tig0020047:69308-69327 None:intergenic 40.0%
!! CTTGCATGCAGTTTGCTTTT+GGG + tig0020047:69049-69068 None:intergenic 40.0%
!! TCTTGCATGCAGTTTGCTTT+TGG + tig0020047:69050-69069 None:intergenic 40.0%
CTGCATGCAAGACAAAGAAA+AGG - tig0020047:69058-69077 Msa1378040:CDS 40.0%
! GACTCAGAACCAACCATTTT+GGG - tig0020047:69093-69112 Msa1378040:CDS 40.0%
GAGTTGCTATCAAGATGCAA+TGG - tig0020047:69228-69247 Msa1378040:CDS 40.0%
TTTGTACAAATGCTCCCTCA+CGG + tig0020047:69308-69327 None:intergenic 40.0%
GCAAGACAAAGAAAAGGGTC+CGG - tig0020047:69064-69083 Msa1378040:CDS 45.0%
! AAAGAAAAGGGTCCGGTTTG+TGG - tig0020047:69071-69090 Msa1378040:CDS 45.0%
!! TTGGTTCTGAGTCCACAAAC+CGG + tig0020047:69086-69105 None:intergenic 45.0%
! GGACTCAGAACCAACCATTT+TGG - tig0020047:69092-69111 Msa1378040:CDS 45.0%
TTCTATTCAAGGGAACCGTG+AGG - tig0020047:69290-69309 Msa1378040:CDS 45.0%
TCTATTCAAGGGAACCGTGA+GGG - tig0020047:69291-69310 Msa1378040:CDS 45.0%
GCAAGACAAAGAAAAGGGTC+CGG - tig0020047:69064-69083 Msa1378040:CDS 45.0%
! AAAGAAAAGGGTCCGGTTTG+TGG - tig0020047:69071-69090 Msa1378040:CDS 45.0%
!! TTGGTTCTGAGTCCACAAAC+CGG + tig0020047:69086-69105 None:intergenic 45.0%
! GGACTCAGAACCAACCATTT+TGG - tig0020047:69092-69111 Msa1378040:CDS 45.0%
TTCTATTCAAGGGAACCGTG+AGG - tig0020047:69290-69309 Msa1378040:CDS 45.0%
TCTATTCAAGGGAACCGTGA+GGG - tig0020047:69291-69310 Msa1378040:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0020047 gene 68961 69341 68961 ID=Msa1378040;Name=Msa1378040
tig0020047 mRNA 68961 69341 68961 ID=Msa1378040-mRNA-1;Parent=Msa1378040;Name=Msa1378040-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|126
tig0020047 exon 68961 69341 68961 ID=Msa1378040-mRNA-1:exon:5956;Parent=Msa1378040-mRNA-1
tig0020047 CDS 68961 69341 68961 ID=Msa1378040-mRNA-1:cds;Parent=Msa1378040-mRNA-1
Gene Sequence

>Msa1378040

ATGTTTGCTTGCCTTAAAAAAATATCACAAAAAAAGATAAAAGTAGAGCAAATTATCAAAGCAACAAAAGATGTATGTGAGTTAACCCAAAAGCAAACTGCATGCAAGACAAAGAAAAGGGTCCGGTTTGTGGACTCAGAACCAACCATTTTGGGAGAAGAAAACAACAATGAAGAATTTGAGAAAAGAAGATGTATTAGTAGTAATGATTTAGTAGAAAAGGAGGGAATTAGAGTGACGATTAGATTAACAAAGGAACAAGCAGCTGAGTTGCTATCAAGATGCAATGGAAGTGTTCTTGAATTGAAGGATGTTGCTCGTGAGTTACTTTCTATTCAAGGGAACCGTGAGGGAGCATTTGTACAAACAATTTCTAGTTAG

Protein sequence

>Msa1378040

MFACLKKISQKKIKVEQIIKATKDVCELTQKQTACKTKKRVRFVDSEPTILGEENNNEEFEKRRCISSNDLVEKEGIRVTIRLTKEQAAELLSRCNGSVLELKDVARELLSIQGNREGAFVQTISS*