AlfalfaGEDB | Msa0846730

M. sativa cultivar zhongmu-4 / Msa0846730

KEGG: -
GO: -
PFAM: PF03763.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0846730	XP_003614009.2	55.556	135	28	1	299	433	210	312	8.71e-31	131
Msa0846730	XP_003614009.2	81.494	308	23	2	1	274	1	308	1.20e-153	447

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0846730	G7JXW7	55.556	135	28	1	299	433	210	312	4.16e-31	131
Msa0846730	G7JXW7	81.494	308	23	2	1	274	1	308	5.71e-154	447

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0164400	Msa0846730	0.815501	8.952604e-52	-8.615850e-47
Msa0194820	Msa0846730	0.823804	1.137316e-53	-8.615850e-47
Msa0194840	Msa0846730	0.823131	1.633698e-53	-8.615850e-47
Msa0388770	Msa0846730	0.805971	1.031438e-49	-8.615850e-47
Msa0418970	Msa0846730	0.806563	7.739603e-50	-8.615850e-47
Msa0435550	Msa0846730	0.801293	9.640726e-49	-8.615850e-47
Msa0562620	Msa0846730	0.803828	2.894101e-49	-8.615850e-47
Msa0579330	Msa0846730	0.800689	1.280790e-48	-8.615850e-47
Msa0616510	Msa0846730	0.837043	6.560648e-57	-8.615850e-47
Msa0650660	Msa0846730	0.804494	2.102783e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0846730	MtrunA17_Chr5g0418651	81.494	308	23	2	1	274	1	308	1.10e-157	447
Msa0846730	MtrunA17_Chr5g0418651	55.556	135	28	1	299	433	210	312	8.01e-35	131
Msa0846730	MtrunA17_Chr4g0042991	36.176	387	126	10	1	272	1	381	3.12e-38	142
Msa0846730	MtrunA17_Chr4g0042991	40.876	137	50	1	297	433	282	387	2.10e-16	80.5
Msa0846730	MtrunA17_Chr5g0418641	84.000	75	11	1	8	81	1	75	3.93e-31	114
Msa0846730	MtrunA17_Chr3g0086051	34.615	104	68	0	169	272	151	254	4.85e-15	75.1
Msa0846730	MtrunA17_Chr5g0418631	50.000	96	35	3	336	423	2	92	1.40e-12	63.9
Msa0846730	MtrunA17_Chr5g0418631	66.667	54	17	1	213	265	36	89	6.19e-12	62.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0846730	AT1G13920.1	39.130	138	51	2	297	433	240	345	1.18e-17	84.3
Msa0846730	AT1G13920.4	39.130	138	51	2	297	433	273	378	1.44e-17	84.3
Msa0846730	AT5G61280.2	34.783	115	73	1	164	278	123	235	3.31e-16	78.2
Msa0846730	AT5G61280.1	34.783	115	73	1	164	278	146	258	5.29e-16	78.2
Msa0846730	AT1G69325.1	44.444	99	55	0	174	272	18	116	2.30e-14	69.7

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Find 88 sgRNAs with CRISPR-Local

Find 350 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTTTCAGGTAAAAC+AGG	0.436606	5_4:+27387490	Msa0846730:CDS
AAATATTACCTTTGAAAGAT+TGG	0.293146	5_4:-27387053	None:intergenic
AACCCTAGCTAATAGTAGAA+TGG	0.508474	5_4:+27386377	Msa0846730:exon
AACTCGACAAGATCAAACAA+AGG	0.689212	5_4:+27388193	Msa0846730:CDS
AAGAAAGGTTGAAACCTTTG+GGG	0.579827	5_4:+27391872	Msa0846730:CDS
AATATTACCTTTGAAAGATT+GGG	0.397926	5_4:-27387052	None:intergenic
AATGAAGAGCTACAAGCAAA+AGG	0.447061	5_4:+27388729	Msa0846730:CDS
AATTGCAGAGTGAAATTGAA+CGG	0.580588	5_4:+27388610	Msa0846730:intron
AATTGCAGAGTGAAATTGAT+CGG	0.561728	5_4:+27391950	Msa0846730:CDS
AATTGGTGGTGGCGGTGGCG+GGG	0.311891	5_4:-27388079	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATTTATGGAGTCAAATG+AGG	+	chr5_4:27386400-27386419	Msa0846730:CDS	30.0%
TTTCTTTCCACACATCTTAT+TGG	+	chr5_4:27386438-27386457	Msa0846730:intron	30.0%
TGATTATGAAGTTTGGATGA+TGG	-	chr5_4:27386706-27386725	None:intergenic	30.0%
CCTTAAAATAAAATGGTGTG+AGG	-	chr5_4:27386748-27386767	None:intergenic	30.0%
TGATATAGCTATCATTGATC+GGG	-	chr5_4:27386817-27386836	None:intergenic	30.0%
AATGAGAGAAATGAAGGTAA+GGG	-	chr5_4:27387092-27387111	None:intergenic	30.0%
ACTAACAATGAGAGAAATGA+AGG	-	chr5_4:27387098-27387117	None:intergenic	30.0%
TATTTCAATCACTCTCCAAT+GGG	-	chr5_4:27387153-27387172	None:intergenic	30.0%
TTATTTCAATCACTCTCCAA+TGG	-	chr5_4:27387154-27387173	None:intergenic	30.0%
GGATAATGCATAATATATGC+AGG	+	chr5_4:27387290-27387309	Msa0846730:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	27386355	27392226	27386355	ID=Msa0846730;Name=Msa0846730
chr5_4	mRNA	27386355	27392226	27386355	ID=Msa0846730-mRNA-1;Parent=Msa0846730;Name=Msa0846730-mRNA-1;_AED=0.16;_eAED=0.16;_QI=41\|0.75\|0.77\|1\|0.87\|0.77\|9\|77\|433
chr5_4	exon	27386355	27386421	27386355	ID=Msa0846730-mRNA-1:exon:10559;Parent=Msa0846730-mRNA-1
chr5_4	exon	27386975	27387066	27386975	ID=Msa0846730-mRNA-1:exon:10560;Parent=Msa0846730-mRNA-1
chr5_4	exon	27387461	27387604	27387461	ID=Msa0846730-mRNA-1:exon:10561;Parent=Msa0846730-mRNA-1
chr5_4	exon	27387914	27388214	27387914	ID=Msa0846730-mRNA-1:exon:10562;Parent=Msa0846730-mRNA-1
chr5_4	exon	27388340	27388421	27388340	ID=Msa0846730-mRNA-1:exon:10563;Parent=Msa0846730-mRNA-1
chr5_4	exon	27388618	27388793	27388618	ID=Msa0846730-mRNA-1:exon:10564;Parent=Msa0846730-mRNA-1
chr5_4	exon	27391254	27391364	27391254	ID=Msa0846730-mRNA-1:exon:10565;Parent=Msa0846730-mRNA-1
chr5_4	exon	27391462	27391543	27391462	ID=Msa0846730-mRNA-1:exon:10566;Parent=Msa0846730-mRNA-1
chr5_4	exon	27391862	27392226	27391862	ID=Msa0846730-mRNA-1:exon:10567;Parent=Msa0846730-mRNA-1
chr5_4	five_prime_UTR	27386355	27386395	27386355	ID=Msa0846730-mRNA-1:five_prime_utr;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27386396	27386421	27386396	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27386975	27387066	27386975	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27387461	27387604	27387461	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27387914	27388214	27387914	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27388340	27388421	27388340	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27388618	27388793	27388618	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27391254	27391364	27391254	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27391462	27391543	27391462	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	CDS	27391862	27392149	27391862	ID=Msa0846730-mRNA-1:cds;Parent=Msa0846730-mRNA-1
chr5_4	three_prime_UTR	27392150	27392226	27392150	ID=Msa0846730-mRNA-1:three_prime_utr;Parent=Msa0846730-mRNA-1

Gene Sequence

>Msa0846730

ATGGAGAATTTATGGAGTCAAATGAGGGTACAACTTACCGGTACGGAGGAGAACAAAGCTGATCTTGGTGCCACAAGAGATCGGAAAATACCTATACAGAAAACCCAATCTTTCAAAGAGAAGAAGAAGGGCCAGAATTGGTTGCAAAAACAGCTTTCAGGTAAAACAGGCCATGATTACGACCACATAGAAATGGTGCATGCTGCAGCAGTAGCTGCCGCTGCATTATCAATTAATTTGCAGGAGACATCTGAGCAGAAGAGTTCATTTAGATCTAAAGATGACCAGGGTGACAAGGCGTCAACATCCTCAGTTGTAGAAGAAAAGAAACCGGAAAAGGCCATTACTCCTACGCCAACAATGAAAAAGACTGCAACTTTCACTGGAAAAAAAACTAACACCACAGCGCCAAATGCTCCTCCTCCACCCCCGCCACCGCCACCACCAATTATGAAGACTTCAGAGATACCTGATCCTCTAAGACAGACTACAACAGGATCAAACAATGGGGAGACAGATGCAGATGAATGGGAGAGAACTGAACTCGACAAGATCAAACAAAGGTATGAGAAGTTGAAAATGTTGATAGATTCCTGGGAAGACAAGAAAAGTATGAAAGCCAAACGCAAGCTAATGAAGCAAGAGAGTGAAATTGAACGGAGAAGGTTAAAAGCTTTGGAGAAATTTCAAAATAAGATGAAGTATGTAAATCTAGTTGCAGACGGCGCAAGAGCAAAGGTCGAGGAAAGCCGAAAAAATGAAGAGCTACAAGCAAAAGGGAAGGCAAGTACAATTCGAACAACGTGCAAACTGCCACGGATGAACAATTTTGTTAGGGAGATAAAAAATGAATGGGAGAGAACTGCGCGTGACATGATCAAACAATGCGCACAGGAATGGGAGATGACTGAGCTCGACAAGATCAAACAAAGGTATGAGAAGTCGAAAGAGCGGATAGATTCCTGGGTAGACAAGAAGAGGAAGAAAGCCAAACGCAAGCTAGCGAAGGAACAGAGTGAACTTGAAGAAAGGTTGAAACCTTTGGGGAAAATTCAAAATAAGATTAAGTATGTAAAACTAGTTGCAAACGGAGCATATGTCAAATTGCAGAGTGAAATTGATCGGCAAAAGTTGAAATCTGTGAAGAAATTTAATAATAAGATGAAGTATGTGAATCTAGTTGCAAAAGGAGCGAGAGACAAGGCTGAGAAAAGACGAGAAAATGAAGAGCTACAAGCAAAAGAGAAGGCAAGTACAATTTCAACAACAGGCAAACTGCCACAGACGTATTTCTGCTTCTGA

Protein sequence

>Msa0846730

MENLWSQMRVQLTGTEENKADLGATRDRKIPIQKTQSFKEKKKGQNWLQKQLSGKTGHDYDHIEMVHAAAVAAAALSINLQETSEQKSSFRSKDDQGDKASTSSVVEEKKPEKAITPTPTMKKTATFTGKKTNTTAPNAPPPPPPPPPPIMKTSEIPDPLRQTTTGSNNGETDADEWERTELDKIKQRYEKLKMLIDSWEDKKSMKAKRKLMKQESEIERRRLKALEKFQNKMKYVNLVADGARAKVEESRKNEELQAKGKASTIRTTCKLPRMNNFVREIKNEWERTARDMIKQCAQEWEMTELDKIKQRYEKSKERIDSWVDKKRKKAKRKLAKEQSELEERLKPLGKIQNKIKYVKLVANGAYVKLQSEIDRQKLKSVKKFNNKMKYVNLVAKGARDKAEKRRENEELQAKEKASTISTTGKLPQTYFCF*