AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650750 MtrunA17_Chr4g0042991 82.216 343 24 3 11 316 45 387 1.09e-171 481
Msa0650750 MtrunA17_Chr5g0418651 45.367 313 128 9 7 316 40 312 3.40e-55 182
Msa0650750 MtrunA17_Chr3g0086051 39.815 108 64 1 209 316 154 260 1.12e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650750 AT1G13920.4 50.943 106 52 0 211 316 273 378 8.71e-29 114
Msa0650750 AT1G13920.1 50.943 106 52 0 211 316 240 345 9.14e-29 113
Msa0650750 AT5G61280.2 28.571 189 125 3 130 316 57 237 1.39e-13 69.7
Msa0650750 AT1G69325.1 42.342 111 63 1 203 313 10 119 2.15e-13 66.2
Msa0650750 AT5G61280.1 28.571 189 125 3 130 316 80 260 2.76e-13 69.3

Find 62 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTACCTGATAATCTTT+TGG 0.246925 4_3:+57995817 None:intergenic
ATAGGCTTTGCTTCTGGTCT+TGG 0.265119 4_3:+57995877 None:intergenic
TACATAAATATACTTATAAT+TGG 0.302158 4_3:+57994403 None:intergenic
GGTTGTGGTGGCGGCGGAGG+TGG 0.327009 4_3:+57995212 None:intergenic
TTTATCATAGGCTTTGCTTC+TGG 0.328813 4_3:+57995871 None:intergenic
TGTGGTGGCGGCGGAGGTGG+TGG 0.339507 4_3:+57995215 None:intergenic
GTTGCACCTGGTCTAGTTGA+TGG 0.341217 4_3:+57995179 None:intergenic
CGGATATCCTAACACCAAAA+AGG 0.345474 4_3:-57995340 Msa0650750:CDS
GCTGCATCCAAAAGATTATC+AGG 0.351559 4_3:-57995824 Msa0650750:intron
GGCGGAGGTGGTGGCGGCTG+CGG 0.379635 4_3:+57995224 None:intergenic
TCTCTAATAGAAGATGTTGT+TGG 0.380294 4_3:+57995464 None:intergenic
TTTGGATGCAGCACCTATAT+GGG 0.393760 4_3:+57995835 None:intergenic
GGAGGTTGTGGTGGCGGCGG+AGG 0.394059 4_3:+57995209 None:intergenic
AATTTCTACAGAGAAGAAAA+AGG 0.405869 4_3:-57996068 Msa0650750:CDS
GCAAACATTATTAGAACAAC+AGG 0.416708 4_3:-57994470 Msa0650750:CDS
CAGAAGCAAAAGCAGATACT+TGG 0.418811 4_3:-57995157 Msa0650750:CDS
GTTCTTTCTTTCTTAGAAGC+AGG 0.419523 4_3:+57994427 None:intergenic
CTGACAGGAGGTTGTGGTGG+CGG 0.433854 4_3:+57995203 None:intergenic
TGTTAGAGACAATAGATTCA+TGG 0.438542 4_3:-57994981 Msa0650750:CDS
AGAACAACAGGAAAGCTTCC+AGG 0.444025 4_3:-57994458 Msa0650750:CDS
AAATCTTTACCACCAAAACT+TGG 0.448320 4_3:+57995314 None:intergenic
GCGGCCATGAGGACGTGGTT+GGG 0.450451 4_3:+57995243 None:intergenic
AAAGTGAAAATACACGGAAA+AGG 0.455987 4_3:-57994610 Msa0650750:CDS
ACAGGAGGTTGTGGTGGCGG+CGG 0.464860 4_3:+57995206 None:intergenic
GGTGGCGGCGGAGGTGGTGG+CGG 0.466003 4_3:+57995218 None:intergenic
TTTATGTACATAATGTCATT+CGG 0.466944 4_3:-57994388 Msa0650750:three_prime_UTR
TTCATTCTTTCGTCTCTCAT+CGG 0.485034 4_3:+57994514 None:intergenic
AAGTGAAAATACACGGAAAA+GGG 0.489919 4_3:-57994609 Msa0650750:CDS
TAGAAGCAGGAACAAGCCCC+TGG 0.500961 4_3:+57994440 None:intergenic
TGCGGCCATGAGGACGTGGT+TGG 0.506565 4_3:+57995242 None:intergenic
GTTAGAGACAATAGATTCAT+GGG 0.510246 4_3:-57994980 Msa0650750:CDS
GATGGTCTGACAGGAGGTTG+TGG 0.513783 4_3:+57995197 None:intergenic
GCTTCTGGTCTTGGCCAATG+AGG 0.527010 4_3:+57995886 None:intergenic
GGTCTAGTTGATGGTCTGAC+AGG 0.535460 4_3:+57995188 None:intergenic
AAAAGCAGATACTTGGGAAA+GGG 0.535761 4_3:-57995150 Msa0650750:CDS
GAACAACAGGAAAGCTTCCA+GGG 0.544421 4_3:-57994457 Msa0650750:CDS
GGAGTTGCCTGAGACCTCAT+TGG 0.544976 4_3:-57995900 Msa0650750:CDS
CGGCTGCGGCCATGAGGACG+TGG 0.549078 4_3:+57995238 None:intergenic
GTCTTGGCCAATGAGGTCTC+AGG 0.549623 4_3:+57995893 None:intergenic
CACGGAAAAGGGAGAAAGCA+TGG 0.551213 4_3:-57994598 Msa0650750:CDS
TTGTCCCAACCACGTCCTCA+TGG 0.559642 4_3:-57995247 Msa0650750:CDS
TTCCACATGAGCAGAAAATG+TGG 0.560420 4_3:-57995922 Msa0650750:CDS
AGAAGCAAAAGCAGATACTT+GGG 0.563207 4_3:-57995156 Msa0650750:CDS
TGATGATGCTGGTGCGAATG+AGG 0.565873 4_3:+57995418 None:intergenic
AACGCTGAAAGCAAAAGATA+AGG 0.570182 4_3:-57994492 Msa0650750:CDS
TGCTGCAGCAGCTACCGCTG+TGG 0.578435 4_3:+57995973 None:intergenic
CAAAAGCAGATACTTGGGAA+AGG 0.582056 4_3:-57995151 Msa0650750:CDS
TGCAGTGTACAAAATCCACT+TGG 0.584272 4_3:-57994291 Msa0650750:three_prime_UTR
CAATAGATTCATGGGAAAAG+CGG 0.584674 4_3:-57994972 Msa0650750:CDS
CACAGATGAACAAGGAAACA+AGG 0.591861 4_3:-57995489 Msa0650750:CDS
TGGTGGCGGCTGCGGCCATG+AGG 0.604575 4_3:+57995232 None:intergenic
TATATAGACGAGATTGCAGA+AGG 0.610293 4_3:-57994551 Msa0650750:CDS
TTTAAAATCACAGATGAACA+AGG 0.610365 4_3:-57995497 Msa0650750:CDS
GGTCTGACAGGAGGTTGTGG+TGG 0.627760 4_3:+57995200 None:intergenic
CTAGTTGATGGTCTGACAGG+AGG 0.633454 4_3:+57995191 None:intergenic
GTCAGACCATCAACTAGACC+AGG 0.640427 4_3:-57995185 Msa0650750:CDS
TGCAGCAAAGTGAAAATACA+CGG 0.640916 4_3:-57994616 Msa0650750:intron
TCCACATGAGCAGAAAATGT+GGG 0.644028 4_3:-57995921 Msa0650750:CDS
ACGCAAGCTAAACAAGCATG+AGG 0.679702 4_3:-57994932 Msa0650750:intron
TGATGCTGGTGCGAATGAGG+TGG 0.686427 4_3:+57995421 None:intergenic
AACAACAGGAAAGCTTCCAG+GGG 0.710912 4_3:-57994456 Msa0650750:CDS
GGAAATGGAGCATGCCACAG+CGG 0.741947 4_3:-57995987 Msa0650750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATAAATATACTTATAAT+TGG + chr4_3:57995871-57995890 None:intergenic 10.0%
!! TACATAAATATACTTATAAT+TGG + chr4_3:57995871-57995890 None:intergenic 10.0%
!!! AAATGAAGATTTTTTATCAT+AGG + chr4_3:57994415-57994434 None:intergenic 15.0%
!!! AAATGAAGATTTTTTATCAT+AGG + chr4_3:57994415-57994434 None:intergenic 15.0%
!!! TGATTATGATTTTATGGAAA+TGG - chr4_3:57994269-57994288 Msa0650750:three_prime_UTR 20.0%
!!! ATATTTACCTGATAATCTTT+TGG + chr4_3:57994457-57994476 None:intergenic 20.0%
!! GAAAATATAATCAATTTGTC+AGG - chr4_3:57995257-57995276 Msa0650750:CDS 20.0%
!! AGTTTAATCAGCTTATAATT+AGG + chr4_3:57995488-57995507 None:intergenic 20.0%
!! TTTATGTACATAATGTCATT+CGG - chr4_3:57995883-57995902 Msa0650750:CDS 20.0%
!!! TGATTATGATTTTATGGAAA+TGG - chr4_3:57994269-57994288 Msa0650750:three_prime_UTR 20.0%
!!! ATATTTACCTGATAATCTTT+TGG + chr4_3:57994457-57994476 None:intergenic 20.0%
!! GAAAATATAATCAATTTGTC+AGG - chr4_3:57995257-57995276 Msa0650750:CDS 20.0%
!! AGTTTAATCAGCTTATAATT+AGG + chr4_3:57995488-57995507 None:intergenic 20.0%
!! TTTATGTACATAATGTCATT+CGG - chr4_3:57995883-57995902 Msa0650750:CDS 20.0%
! AATTTCTACAGAGAAGAAAA+AGG - chr4_3:57994203-57994222 Msa0650750:three_prime_UTR 25.0%
!! GAGTCATGATTATGATTTTA+TGG - chr4_3:57994263-57994282 Msa0650750:three_prime_UTR 25.0%
!! AAATCTTCATTTTCCCATAT+AGG - chr4_3:57994423-57994442 Msa0650750:exon 25.0%
! TTTAACGTTCTTGTATTCTT+AGG - chr4_3:57994594-57994613 Msa0650750:CDS 25.0%
! TTAACGTTCTTGTATTCTTA+GGG - chr4_3:57994595-57994614 Msa0650750:CDS 25.0%
! TGTATTCTTAGGGATAAATT+CGG - chr4_3:57994605-57994624 Msa0650750:CDS 25.0%
!! TTTAAAATCACAGATGAACA+AGG - chr4_3:57994774-57994793 Msa0650750:intron 25.0%
! CTTCTATTAGAGAAGAAAAA+AGG - chr4_3:57994817-57994836 Msa0650750:intron 25.0%
!!! TTTGATTTTTCACCAAAAGT+AGG + chr4_3:57994891-57994910 None:intergenic 25.0%
! GAGAAATGATGATAAAAAAC+CGG - chr4_3:57994911-57994930 Msa0650750:intron 25.0%
!!! TTGGTTTTTCTTCTTTTCTA+AGG - chr4_3:57995999-57996018 Msa0650750:CDS 25.0%
! AATTTCTACAGAGAAGAAAA+AGG - chr4_3:57994203-57994222 Msa0650750:three_prime_UTR 25.0%
!! GAGTCATGATTATGATTTTA+TGG - chr4_3:57994263-57994282 Msa0650750:three_prime_UTR 25.0%
!! AAATCTTCATTTTCCCATAT+AGG - chr4_3:57994423-57994442 Msa0650750:exon 25.0%
! TTTAACGTTCTTGTATTCTT+AGG - chr4_3:57994594-57994613 Msa0650750:CDS 25.0%
! TTAACGTTCTTGTATTCTTA+GGG - chr4_3:57994595-57994614 Msa0650750:CDS 25.0%
! TGTATTCTTAGGGATAAATT+CGG - chr4_3:57994605-57994624 Msa0650750:CDS 25.0%
!! TTTAAAATCACAGATGAACA+AGG - chr4_3:57994774-57994793 Msa0650750:intron 25.0%
! CTTCTATTAGAGAAGAAAAA+AGG - chr4_3:57994817-57994836 Msa0650750:intron 25.0%
!!! TTTGATTTTTCACCAAAAGT+AGG + chr4_3:57994891-57994910 None:intergenic 25.0%
! GAGAAATGATGATAAAAAAC+CGG - chr4_3:57994911-57994930 Msa0650750:intron 25.0%
!!! TTGGTTTTTCTTCTTTTCTA+AGG - chr4_3:57995999-57996018 Msa0650750:CDS 25.0%
! AGAAGAAAAAGGCACATAAT+TGG - chr4_3:57994214-57994233 Msa0650750:three_prime_UTR 30.0%
AGAACGTTAAAAACTCTTGA+GGG + chr4_3:57994585-57994604 None:intergenic 30.0%
AAGAACGTTAAAAACTCTTG+AGG + chr4_3:57994586-57994605 None:intergenic 30.0%
TCTCTAATAGAAGATGTTGT+TGG + chr4_3:57994810-57994829 None:intergenic 30.0%
! ATGACAAAAACTCCTACTTT+TGG - chr4_3:57994876-57994895 Msa0650750:intron 30.0%
AAATCTTTACCACCAAAACT+TGG + chr4_3:57994960-57994979 None:intergenic 30.0%
!!! TTGGGACAATTTTTGGTTTT+GGG + chr4_3:57995013-57995032 None:intergenic 30.0%
AGCTCAAAAAGATCAAAGAA+AGG - chr4_3:57995147-57995166 Msa0650750:CDS 30.0%
TGTTAGAGACAATAGATTCA+TGG - chr4_3:57995290-57995309 Msa0650750:CDS 30.0%
GTTAGAGACAATAGATTCAT+GGG - chr4_3:57995291-57995310 Msa0650750:CDS 30.0%
! GAGGTTAGATTTTCATATTC+AGG - chr4_3:57995358-57995377 Msa0650750:CDS 30.0%
TTAAAGTGGTTATCCAAACA+GGG + chr4_3:57995384-57995403 None:intergenic 30.0%
ATTAAAGTGGTTATCCAAAC+AGG + chr4_3:57995385-57995404 None:intergenic 30.0%
!!! GAGAATTAACAAGAGCATTT+TGG + chr4_3:57995606-57995625 None:intergenic 30.0%
GTTAATTCTCTGATAAAGTG+TGG - chr4_3:57995616-57995635 Msa0650750:intron 30.0%
AAAGTGAAAATACACGGAAA+AGG - chr4_3:57995661-57995680 Msa0650750:intron 30.0%
AAGTGAAAATACACGGAAAA+GGG - chr4_3:57995662-57995681 Msa0650750:intron 30.0%
GCAAACATTATTAGAACAAC+AGG - chr4_3:57995801-57995820 Msa0650750:intron 30.0%
AGAAAAGAAGAAAAACCAAG+TGG + chr4_3:57995998-57996017 None:intergenic 30.0%
! AGAAGAAAAAGGCACATAAT+TGG - chr4_3:57994214-57994233 Msa0650750:three_prime_UTR 30.0%
AGAACGTTAAAAACTCTTGA+GGG + chr4_3:57994585-57994604 None:intergenic 30.0%
AAGAACGTTAAAAACTCTTG+AGG + chr4_3:57994586-57994605 None:intergenic 30.0%
TCTCTAATAGAAGATGTTGT+TGG + chr4_3:57994810-57994829 None:intergenic 30.0%
! ATGACAAAAACTCCTACTTT+TGG - chr4_3:57994876-57994895 Msa0650750:intron 30.0%
AAATCTTTACCACCAAAACT+TGG + chr4_3:57994960-57994979 None:intergenic 30.0%
!!! TTGGGACAATTTTTGGTTTT+GGG + chr4_3:57995013-57995032 None:intergenic 30.0%
AGCTCAAAAAGATCAAAGAA+AGG - chr4_3:57995147-57995166 Msa0650750:CDS 30.0%
TGTTAGAGACAATAGATTCA+TGG - chr4_3:57995290-57995309 Msa0650750:CDS 30.0%
GTTAGAGACAATAGATTCAT+GGG - chr4_3:57995291-57995310 Msa0650750:CDS 30.0%
! GAGGTTAGATTTTCATATTC+AGG - chr4_3:57995358-57995377 Msa0650750:CDS 30.0%
TTAAAGTGGTTATCCAAACA+GGG + chr4_3:57995384-57995403 None:intergenic 30.0%
ATTAAAGTGGTTATCCAAAC+AGG + chr4_3:57995385-57995404 None:intergenic 30.0%
!!! GAGAATTAACAAGAGCATTT+TGG + chr4_3:57995606-57995625 None:intergenic 30.0%
GTTAATTCTCTGATAAAGTG+TGG - chr4_3:57995616-57995635 Msa0650750:intron 30.0%
AAAGTGAAAATACACGGAAA+AGG - chr4_3:57995661-57995680 Msa0650750:intron 30.0%
AAGTGAAAATACACGGAAAA+GGG - chr4_3:57995662-57995681 Msa0650750:intron 30.0%
GCAAACATTATTAGAACAAC+AGG - chr4_3:57995801-57995820 Msa0650750:intron 30.0%
AGAAAAGAAGAAAAACCAAG+TGG + chr4_3:57995998-57996017 None:intergenic 30.0%
! GGTTTCAGAGACAATTTTCA+AGG - chr4_3:57994235-57994254 Msa0650750:three_prime_UTR 35.0%
TTTATCATAGGCTTTGCTTC+TGG + chr4_3:57994403-57994422 None:intergenic 35.0%
ACTCAATATGCATCACTTGT+AGG - chr4_3:57994747-57994766 Msa0650750:intron 35.0%
!!! GTTTTTGTCATTGATGATGC+TGG + chr4_3:57994867-57994886 None:intergenic 35.0%
!!! CTTTTTGGTGTTAGGATATC+CGG + chr4_3:57994933-57994952 None:intergenic 35.0%
!!! TGGGACAATTTTTGGTTTTG+GGG + chr4_3:57995012-57995031 None:intergenic 35.0%
!!! GTTGGGACAATTTTTGGTTT+TGG + chr4_3:57995014-57995033 None:intergenic 35.0%
AGAAGCAAAAGCAGATACTT+GGG - chr4_3:57995115-57995134 Msa0650750:intron 35.0%
AAAAGCAGATACTTGGGAAA+GGG - chr4_3:57995121-57995140 Msa0650750:intron 35.0%
CAATAGATTCATGGGAAAAG+CGG - chr4_3:57995299-57995318 Msa0650750:CDS 35.0%
TGCAGCAAAGTGAAAATACA+CGG - chr4_3:57995655-57995674 Msa0650750:intron 35.0%
TATATAGACGAGATTGCAGA+AGG - chr4_3:57995720-57995739 Msa0650750:intron 35.0%
TTCATTCTTTCGTCTCTCAT+CGG + chr4_3:57995760-57995779 None:intergenic 35.0%
AACGCTGAAAGCAAAAGATA+AGG - chr4_3:57995779-57995798 Msa0650750:intron 35.0%
GTTCTTTCTTTCTTAGAAGC+AGG + chr4_3:57995847-57995866 None:intergenic 35.0%
! GGTTTCAGAGACAATTTTCA+AGG - chr4_3:57994235-57994254 Msa0650750:three_prime_UTR 35.0%
TTTATCATAGGCTTTGCTTC+TGG + chr4_3:57994403-57994422 None:intergenic 35.0%
ACTCAATATGCATCACTTGT+AGG - chr4_3:57994747-57994766 Msa0650750:intron 35.0%
!!! GTTTTTGTCATTGATGATGC+TGG + chr4_3:57994867-57994886 None:intergenic 35.0%
!!! CTTTTTGGTGTTAGGATATC+CGG + chr4_3:57994933-57994952 None:intergenic 35.0%
!!! TGGGACAATTTTTGGTTTTG+GGG + chr4_3:57995012-57995031 None:intergenic 35.0%
!!! GTTGGGACAATTTTTGGTTT+TGG + chr4_3:57995014-57995033 None:intergenic 35.0%
AGAAGCAAAAGCAGATACTT+GGG - chr4_3:57995115-57995134 Msa0650750:intron 35.0%
AAAAGCAGATACTTGGGAAA+GGG - chr4_3:57995121-57995140 Msa0650750:intron 35.0%
CAATAGATTCATGGGAAAAG+CGG - chr4_3:57995299-57995318 Msa0650750:CDS 35.0%
TGCAGCAAAGTGAAAATACA+CGG - chr4_3:57995655-57995674 Msa0650750:intron 35.0%
TATATAGACGAGATTGCAGA+AGG - chr4_3:57995720-57995739 Msa0650750:intron 35.0%
TTCATTCTTTCGTCTCTCAT+CGG + chr4_3:57995760-57995779 None:intergenic 35.0%
AACGCTGAAAGCAAAAGATA+AGG - chr4_3:57995779-57995798 Msa0650750:intron 35.0%
GTTCTTTCTTTCTTAGAAGC+AGG + chr4_3:57995847-57995866 None:intergenic 35.0%
TTCCACATGAGCAGAAAATG+TGG - chr4_3:57994349-57994368 Msa0650750:three_prime_UTR 40.0%
TCCACATGAGCAGAAAATGT+GGG - chr4_3:57994350-57994369 Msa0650750:three_prime_UTR 40.0%
TTTGGATGCAGCACCTATAT+GGG + chr4_3:57994439-57994458 None:intergenic 40.0%
! TTTTGGATGCAGCACCTATA+TGG + chr4_3:57994440-57994459 None:intergenic 40.0%
GCTGCATCCAAAAGATTATC+AGG - chr4_3:57994447-57994466 Msa0650750:CDS 40.0%
CACAGATGAACAAGGAAACA+AGG - chr4_3:57994782-57994801 Msa0650750:intron 40.0%
CGGATATCCTAACACCAAAA+AGG - chr4_3:57994931-57994950 Msa0650750:intron 40.0%
!!! TTGGAGTCCTTTTTGGTGTT+AGG + chr4_3:57994941-57994960 None:intergenic 40.0%
! CCAAAACTTGGAGTCCTTTT+TGG + chr4_3:57994948-57994967 None:intergenic 40.0%
! CCAAAAAGGACTCCAAGTTT+TGG - chr4_3:57994945-57994964 Msa0650750:CDS 40.0%
!! AAAAGGACTCCAAGTTTTGG+TGG - chr4_3:57994948-57994967 Msa0650750:CDS 40.0%
!!! ATTTTTGGTTTTGGGGTCTC+AGG + chr4_3:57995005-57995024 None:intergenic 40.0%
CAGAAGCAAAAGCAGATACT+TGG - chr4_3:57995114-57995133 Msa0650750:intron 40.0%
CAAAAGCAGATACTTGGGAA+AGG - chr4_3:57995120-57995139 Msa0650750:intron 40.0%
TTTCATATTCAGGCCCTGTT+TGG - chr4_3:57995368-57995387 Msa0650750:CDS 40.0%
GTGTAAGCGCTCAATTAAAG+TGG + chr4_3:57995398-57995417 None:intergenic 40.0%
TGCAGTGTACAAAATCCACT+TGG - chr4_3:57995980-57995999 Msa0650750:CDS 40.0%
TTCCACATGAGCAGAAAATG+TGG - chr4_3:57994349-57994368 Msa0650750:three_prime_UTR 40.0%
TCCACATGAGCAGAAAATGT+GGG - chr4_3:57994350-57994369 Msa0650750:three_prime_UTR 40.0%
TTTGGATGCAGCACCTATAT+GGG + chr4_3:57994439-57994458 None:intergenic 40.0%
! TTTTGGATGCAGCACCTATA+TGG + chr4_3:57994440-57994459 None:intergenic 40.0%
GCTGCATCCAAAAGATTATC+AGG - chr4_3:57994447-57994466 Msa0650750:CDS 40.0%
CACAGATGAACAAGGAAACA+AGG - chr4_3:57994782-57994801 Msa0650750:intron 40.0%
CGGATATCCTAACACCAAAA+AGG - chr4_3:57994931-57994950 Msa0650750:intron 40.0%
!!! TTGGAGTCCTTTTTGGTGTT+AGG + chr4_3:57994941-57994960 None:intergenic 40.0%
! CCAAAACTTGGAGTCCTTTT+TGG + chr4_3:57994948-57994967 None:intergenic 40.0%
! CCAAAAAGGACTCCAAGTTT+TGG - chr4_3:57994945-57994964 Msa0650750:CDS 40.0%
!! AAAAGGACTCCAAGTTTTGG+TGG - chr4_3:57994948-57994967 Msa0650750:CDS 40.0%
!!! ATTTTTGGTTTTGGGGTCTC+AGG + chr4_3:57995005-57995024 None:intergenic 40.0%
CAGAAGCAAAAGCAGATACT+TGG - chr4_3:57995114-57995133 Msa0650750:intron 40.0%
CAAAAGCAGATACTTGGGAA+AGG - chr4_3:57995120-57995139 Msa0650750:intron 40.0%
TTTCATATTCAGGCCCTGTT+TGG - chr4_3:57995368-57995387 Msa0650750:CDS 40.0%
GTGTAAGCGCTCAATTAAAG+TGG + chr4_3:57995398-57995417 None:intergenic 40.0%
TGCAGTGTACAAAATCCACT+TGG - chr4_3:57995980-57995999 Msa0650750:CDS 40.0%
! TCCCACATTTTCTGCTCATG+TGG + chr4_3:57994354-57994373 None:intergenic 45.0%
ATAGGCTTTGCTTCTGGTCT+TGG + chr4_3:57994397-57994416 None:intergenic 45.0%
CTGAGTAGAACCACAATGTG+CGG - chr4_3:57994550-57994569 Msa0650750:CDS 45.0%
AATTCGGACTCACTACCTCT+TGG - chr4_3:57994621-57994640 Msa0650750:intron 45.0%
CAAGTCTCTTTCAGTCCAAG+AGG + chr4_3:57994639-57994658 None:intergenic 45.0%
! GACGTGGTTGGGACAATTTT+TGG + chr4_3:57995020-57995039 None:intergenic 45.0%
ACGCAAGCTAAACAAGCATG+AGG - chr4_3:57995339-57995358 Msa0650750:CDS 45.0%
AGAACAACAGGAAAGCTTCC+AGG - chr4_3:57995813-57995832 Msa0650750:intron 45.0%
GAACAACAGGAAAGCTTCCA+GGG - chr4_3:57995814-57995833 Msa0650750:intron 45.0%
AACAACAGGAAAGCTTCCAG+GGG - chr4_3:57995815-57995834 Msa0650750:intron 45.0%
! TCCCACATTTTCTGCTCATG+TGG + chr4_3:57994354-57994373 None:intergenic 45.0%
ATAGGCTTTGCTTCTGGTCT+TGG + chr4_3:57994397-57994416 None:intergenic 45.0%
CTGAGTAGAACCACAATGTG+CGG - chr4_3:57994550-57994569 Msa0650750:CDS 45.0%
AATTCGGACTCACTACCTCT+TGG - chr4_3:57994621-57994640 Msa0650750:intron 45.0%
CAAGTCTCTTTCAGTCCAAG+AGG + chr4_3:57994639-57994658 None:intergenic 45.0%
! GACGTGGTTGGGACAATTTT+TGG + chr4_3:57995020-57995039 None:intergenic 45.0%
ACGCAAGCTAAACAAGCATG+AGG - chr4_3:57995339-57995358 Msa0650750:CDS 45.0%
AGAACAACAGGAAAGCTTCC+AGG - chr4_3:57995813-57995832 Msa0650750:intron 45.0%
GAACAACAGGAAAGCTTCCA+GGG - chr4_3:57995814-57995833 Msa0650750:intron 45.0%
AACAACAGGAAAGCTTCCAG+GGG - chr4_3:57995815-57995834 Msa0650750:intron 45.0%
! TGTGGTTCTACTCAGCTCGA+TGG + chr4_3:57994545-57994564 None:intergenic 50.0%
TAGAACCACAATGTGCGGCA+AGG - chr4_3:57994555-57994574 Msa0650750:CDS 50.0%
! GCGAATGAGGTGGTCTTTTC+AGG + chr4_3:57994843-57994862 None:intergenic 50.0%
! TGATGATGCTGGTGCGAATG+AGG + chr4_3:57994856-57994875 None:intergenic 50.0%
!! CTAGTTGATGGTCTGACAGG+AGG + chr4_3:57995083-57995102 None:intergenic 50.0%
!! GGTCTAGTTGATGGTCTGAC+AGG + chr4_3:57995086-57995105 None:intergenic 50.0%
GTCAGACCATCAACTAGACC+AGG - chr4_3:57995086-57995105 Msa0650750:intron 50.0%
GTTGCACCTGGTCTAGTTGA+TGG + chr4_3:57995095-57995114 None:intergenic 50.0%
! GCTTTTGCTTCTGTTGCACC+TGG + chr4_3:57995107-57995126 None:intergenic 50.0%
CACGGAAAAGGGAGAAAGCA+TGG - chr4_3:57995673-57995692 Msa0650750:intron 50.0%
! TGTGGTTCTACTCAGCTCGA+TGG + chr4_3:57994545-57994564 None:intergenic 50.0%
TAGAACCACAATGTGCGGCA+AGG - chr4_3:57994555-57994574 Msa0650750:CDS 50.0%
! GCGAATGAGGTGGTCTTTTC+AGG + chr4_3:57994843-57994862 None:intergenic 50.0%
! TGATGATGCTGGTGCGAATG+AGG + chr4_3:57994856-57994875 None:intergenic 50.0%
!! CTAGTTGATGGTCTGACAGG+AGG + chr4_3:57995083-57995102 None:intergenic 50.0%
!! GGTCTAGTTGATGGTCTGAC+AGG + chr4_3:57995086-57995105 None:intergenic 50.0%
GTCAGACCATCAACTAGACC+AGG - chr4_3:57995086-57995105 Msa0650750:intron 50.0%
GTTGCACCTGGTCTAGTTGA+TGG + chr4_3:57995095-57995114 None:intergenic 50.0%
! GCTTTTGCTTCTGTTGCACC+TGG + chr4_3:57995107-57995126 None:intergenic 50.0%
CACGGAAAAGGGAGAAAGCA+TGG - chr4_3:57995673-57995692 Msa0650750:intron 50.0%
GGAAATGGAGCATGCCACAG+CGG - chr4_3:57994284-57994303 Msa0650750:three_prime_UTR 55.0%
GGAGTTGCCTGAGACCTCAT+TGG - chr4_3:57994371-57994390 Msa0650750:three_prime_UTR 55.0%
GTCTTGGCCAATGAGGTCTC+AGG + chr4_3:57994381-57994400 None:intergenic 55.0%
!! GCTTCTGGTCTTGGCCAATG+AGG + chr4_3:57994388-57994407 None:intergenic 55.0%
TGATGCTGGTGCGAATGAGG+TGG + chr4_3:57994853-57994872 None:intergenic 55.0%
TTGTCCCAACCACGTCCTCA+TGG - chr4_3:57995024-57995043 Msa0650750:intron 55.0%
GATGGTCTGACAGGAGGTTG+TGG + chr4_3:57995077-57995096 None:intergenic 55.0%
TAGAAGCAGGAACAAGCCCC+TGG + chr4_3:57995834-57995853 None:intergenic 55.0%
GGAAATGGAGCATGCCACAG+CGG - chr4_3:57994284-57994303 Msa0650750:three_prime_UTR 55.0%
GGAGTTGCCTGAGACCTCAT+TGG - chr4_3:57994371-57994390 Msa0650750:three_prime_UTR 55.0%
GTCTTGGCCAATGAGGTCTC+AGG + chr4_3:57994381-57994400 None:intergenic 55.0%
!! GCTTCTGGTCTTGGCCAATG+AGG + chr4_3:57994388-57994407 None:intergenic 55.0%
TGATGCTGGTGCGAATGAGG+TGG + chr4_3:57994853-57994872 None:intergenic 55.0%
TTGTCCCAACCACGTCCTCA+TGG - chr4_3:57995024-57995043 Msa0650750:intron 55.0%
GATGGTCTGACAGGAGGTTG+TGG + chr4_3:57995077-57995096 None:intergenic 55.0%
TAGAAGCAGGAACAAGCCCC+TGG + chr4_3:57995834-57995853 None:intergenic 55.0%
GAGAGCCTTGCCGCACATTG+TGG + chr4_3:57994563-57994582 None:intergenic 60.0%
CTGACAGGAGGTTGTGGTGG+CGG + chr4_3:57995071-57995090 None:intergenic 60.0%
GGTCTGACAGGAGGTTGTGG+TGG + chr4_3:57995074-57995093 None:intergenic 60.0%
GAGAGCCTTGCCGCACATTG+TGG + chr4_3:57994563-57994582 None:intergenic 60.0%
CTGACAGGAGGTTGTGGTGG+CGG + chr4_3:57995071-57995090 None:intergenic 60.0%
GGTCTGACAGGAGGTTGTGG+TGG + chr4_3:57995074-57995093 None:intergenic 60.0%
TGCTGCAGCAGCTACCGCTG+TGG + chr4_3:57994301-57994320 None:intergenic 65.0%
GCGGCCATGAGGACGTGGTT+GGG + chr4_3:57995031-57995050 None:intergenic 65.0%
TGCGGCCATGAGGACGTGGT+TGG + chr4_3:57995032-57995051 None:intergenic 65.0%
!! ACAGGAGGTTGTGGTGGCGG+CGG + chr4_3:57995068-57995087 None:intergenic 65.0%
TGCTGCAGCAGCTACCGCTG+TGG + chr4_3:57994301-57994320 None:intergenic 65.0%
GCGGCCATGAGGACGTGGTT+GGG + chr4_3:57995031-57995050 None:intergenic 65.0%
TGCGGCCATGAGGACGTGGT+TGG + chr4_3:57995032-57995051 None:intergenic 65.0%
!! ACAGGAGGTTGTGGTGGCGG+CGG + chr4_3:57995068-57995087 None:intergenic 65.0%
CGGCTGCGGCCATGAGGACG+TGG + chr4_3:57995036-57995055 None:intergenic 75.0%
!! TGGTGGCGGCTGCGGCCATG+AGG + chr4_3:57995042-57995061 None:intergenic 75.0%
!! TGTGGTGGCGGCGGAGGTGG+TGG + chr4_3:57995059-57995078 None:intergenic 75.0%
!! GGTTGTGGTGGCGGCGGAGG+TGG + chr4_3:57995062-57995081 None:intergenic 75.0%
!! GGAGGTTGTGGTGGCGGCGG+AGG + chr4_3:57995065-57995084 None:intergenic 75.0%
CGGCTGCGGCCATGAGGACG+TGG + chr4_3:57995036-57995055 None:intergenic 75.0%
!! TGGTGGCGGCTGCGGCCATG+AGG + chr4_3:57995042-57995061 None:intergenic 75.0%
!! TGTGGTGGCGGCGGAGGTGG+TGG + chr4_3:57995059-57995078 None:intergenic 75.0%
!! GGTTGTGGTGGCGGCGGAGG+TGG + chr4_3:57995062-57995081 None:intergenic 75.0%
!! GGAGGTTGTGGTGGCGGCGG+AGG + chr4_3:57995065-57995084 None:intergenic 75.0%
!!! GGCGGAGGTGGTGGCGGCTG+CGG + chr4_3:57995050-57995069 None:intergenic 80.0%
!! GGTGGCGGCGGAGGTGGTGG+CGG + chr4_3:57995056-57995075 None:intergenic 80.0%
!!! GGCGGAGGTGGTGGCGGCTG+CGG + chr4_3:57995050-57995069 None:intergenic 80.0%
!! GGTGGCGGCGGAGGTGGTGG+CGG + chr4_3:57995056-57995075 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 57994195 57996098 57994195 ID=Msa0650750;Name=Msa0650750
chr4_3 mRNA 57994195 57996098 57994195 ID=Msa0650750-mRNA-1;Parent=Msa0650750;Name=Msa0650750-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.75|1|1|1|4|244|316
chr4_3 exon 57994195 57994633 57994195 ID=Msa0650750-mRNA-1:exon:15287;Parent=Msa0650750-mRNA-1
chr4_3 exon 57994933 57995014 57994933 ID=Msa0650750-mRNA-1:exon:15286;Parent=Msa0650750-mRNA-1
chr4_3 exon 57995125 57995524 57995125 ID=Msa0650750-mRNA-1:exon:15285;Parent=Msa0650750-mRNA-1
chr4_3 exon 57995825 57996098 57995825 ID=Msa0650750-mRNA-1:exon:15284;Parent=Msa0650750-mRNA-1
chr4_3 CDS 57995825 57996098 57995825 ID=Msa0650750-mRNA-1:cds;Parent=Msa0650750-mRNA-1
chr4_3 CDS 57995125 57995524 57995125 ID=Msa0650750-mRNA-1:cds;Parent=Msa0650750-mRNA-1
chr4_3 CDS 57994933 57995014 57994933 ID=Msa0650750-mRNA-1:cds;Parent=Msa0650750-mRNA-1
chr4_3 CDS 57994439 57994633 57994439 ID=Msa0650750-mRNA-1:cds;Parent=Msa0650750-mRNA-1
chr4_3 three_prime_UTR 57994195 57994438 57994195 ID=Msa0650750-mRNA-1:three_prime_utr;Parent=Msa0650750-mRNA-1
Gene Sequence

>Msa0650750

ATGTCTGCAATTTCTACAGAGAAGAAAAAGGCACATAATTGGTTTCAGAGACAATTTTCAAGGAATATGAGTCATGATTATGATTTTATGGAAATGGAGCATGCCACAGCGGTAGCTGCTGCAGCATTTGCAATTAGTTCACATGTCTCAGAAATTCCACATGAGCAGAAAATGTGGGAGTTGCCTGAGACCTCATTGGCCAAGACCAGAAGCAAAGCCTATGATAAAAAATCTTCATTTTCCCATATAGGTGCTGCATCCAAAAGATTATCAGGCTCATTTAAAATCACAGATGAACAAGGAAACAAGGTGCCAACAACATCTTCTATTAGAGAAGAAAAAAGGCCTGAAAAGACCACCTCATTCGCACCAGCATCATCAATGACAAAAACTCCTACTTTTGGTGAAAAATCAAAGAGAAATGATGATAAAAAACCGGATATCCTAACACCAAAAAGGACTCCAAGTTTTGGTGGTAAAGATTTTATTAACACTGATGATATACAACCTGAGACCCCAAAACCAAAAATTGTCCCAACCACGTCCTCATGGCCGCAGCCGCCACCACCTCCGCCGCCACCACAACCTCCTGTCAGACCATCAACTAGACCAGGTGCAACAGAAGCAAAAGCAGATACTTGGGAAAGGGAAGAGCTCAAAAAGATCAAAGAAAGGTATGAGAAGTTGTTAGAGACAATAGATTCATGGGAAAAGCGGAAGAAGATGAAAGCAAGACGCAAGCTAAACAAGCATGAGCAAAGTGAAAATACACGGAAAAGGGAGAAAGCATGGAAAAAATATCAAGATAAGATTAAATATATAGACGAGATTGCAGAAGGAGCAAGAGCACAGTCCGATGAGAGACGAAAGAATGAAACGCTGAAAGCAAAAGATAAGGCAAACATTATTAGAACAACAGGAAAGCTTCCAGGGGCTTGTTCCTGCTTCTAA
Protein sequence

>Msa0650750

MSAISTEKKKAHNWFQRQFSRNMSHDYDFMEMEHATAVAAAAFAISSHVSEIPHEQKMWELPETSLAKTRSKAYDKKSSFSHIGAASKRLSGSFKITDEQGNKVPTTSSIREEKRPEKTTSFAPASSMTKTPTFGEKSKRNDDKKPDILTPKRTPSFGGKDFINTDDIQPETPKPKIVPTTSSWPQPPPPPPPPQPPVRPSTRPGATEAKADTWEREELKKIKERYEKLLETIDSWEKRKKMKARRKLNKHEQSENTRKREKAWKKYQDKIKYIDEIAEGARAQSDERRKNETLKAKDKANIIRTTGKLPGACSCF*