AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650660 MtrunA17_Chr4g0042991 82.216 343 24 3 11 316 45 387 1.09e-171 481
Msa0650660 MtrunA17_Chr5g0418651 45.367 313 128 9 7 316 40 312 3.40e-55 182
Msa0650660 MtrunA17_Chr3g0086051 39.815 108 64 1 209 316 154 260 1.12e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650660 AT1G13920.4 50.943 106 52 0 211 316 273 378 8.71e-29 114
Msa0650660 AT1G13920.1 50.943 106 52 0 211 316 240 345 9.14e-29 113
Msa0650660 AT5G61280.2 28.571 189 125 3 130 316 57 237 1.39e-13 69.7
Msa0650660 AT1G69325.1 42.342 111 63 1 203 313 10 119 2.15e-13 66.2
Msa0650660 AT5G61280.1 28.571 189 125 3 130 316 80 260 2.76e-13 69.3

Find 62 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTACCTGATAATCTTT+TGG 0.246925 4_3:+57884094 None:intergenic
ATAGGCTTTGCTTCTGGTCT+TGG 0.265119 4_3:+57884154 None:intergenic
TACATAAATATACTTATAAT+TGG 0.302158 4_3:+57882680 None:intergenic
GGTTGTGGTGGCGGCGGAGG+TGG 0.327009 4_3:+57883489 None:intergenic
TTTATCATAGGCTTTGCTTC+TGG 0.328813 4_3:+57884148 None:intergenic
TGTGGTGGCGGCGGAGGTGG+TGG 0.339507 4_3:+57883492 None:intergenic
GTTGCACCTGGTCTAGTTGA+TGG 0.341217 4_3:+57883456 None:intergenic
CGGATATCCTAACACCAAAA+AGG 0.345474 4_3:-57883617 Msa0650660:CDS
GCTGCATCCAAAAGATTATC+AGG 0.351559 4_3:-57884101 Msa0650660:intron
GGCGGAGGTGGTGGCGGCTG+CGG 0.379635 4_3:+57883501 None:intergenic
TCTCTAATAGAAGATGTTGT+TGG 0.380294 4_3:+57883741 None:intergenic
TTTGGATGCAGCACCTATAT+GGG 0.393760 4_3:+57884112 None:intergenic
GGAGGTTGTGGTGGCGGCGG+AGG 0.394059 4_3:+57883486 None:intergenic
AATTTCTACAGAGAAGAAAA+AGG 0.405869 4_3:-57884345 Msa0650660:CDS
GCAAACATTATTAGAACAAC+AGG 0.416708 4_3:-57882747 Msa0650660:CDS
CAGAAGCAAAAGCAGATACT+TGG 0.418811 4_3:-57883434 Msa0650660:CDS
GTTCTTTCTTTCTTAGAAGC+AGG 0.419523 4_3:+57882704 None:intergenic
CTGACAGGAGGTTGTGGTGG+CGG 0.433854 4_3:+57883480 None:intergenic
TGTTAGAGACAATAGATTCA+TGG 0.438542 4_3:-57883258 Msa0650660:CDS
AGAACAACAGGAAAGCTTCC+AGG 0.444025 4_3:-57882735 Msa0650660:CDS
AAATCTTTACCACCAAAACT+TGG 0.448320 4_3:+57883591 None:intergenic
GCGGCCATGAGGACGTGGTT+GGG 0.450451 4_3:+57883520 None:intergenic
AAAGTGAAAATACACGGAAA+AGG 0.455987 4_3:-57882887 Msa0650660:CDS
ACAGGAGGTTGTGGTGGCGG+CGG 0.464860 4_3:+57883483 None:intergenic
GGTGGCGGCGGAGGTGGTGG+CGG 0.466003 4_3:+57883495 None:intergenic
TTTATGTACATAATGTCATT+CGG 0.466944 4_3:-57882665 Msa0650660:three_prime_UTR
TTCATTCTTTCGTCTCTCAT+CGG 0.485034 4_3:+57882791 None:intergenic
AAGTGAAAATACACGGAAAA+GGG 0.489919 4_3:-57882886 Msa0650660:CDS
TAGAAGCAGGAACAAGCCCC+TGG 0.500961 4_3:+57882717 None:intergenic
TGCGGCCATGAGGACGTGGT+TGG 0.506565 4_3:+57883519 None:intergenic
GTTAGAGACAATAGATTCAT+GGG 0.510246 4_3:-57883257 Msa0650660:CDS
GATGGTCTGACAGGAGGTTG+TGG 0.513783 4_3:+57883474 None:intergenic
GCTTCTGGTCTTGGCCAATG+AGG 0.527010 4_3:+57884163 None:intergenic
GGTCTAGTTGATGGTCTGAC+AGG 0.535460 4_3:+57883465 None:intergenic
AAAAGCAGATACTTGGGAAA+GGG 0.535761 4_3:-57883427 Msa0650660:CDS
GAACAACAGGAAAGCTTCCA+GGG 0.544421 4_3:-57882734 Msa0650660:CDS
GGAGTTGCCTGAGACCTCAT+TGG 0.544976 4_3:-57884177 Msa0650660:CDS
CGGCTGCGGCCATGAGGACG+TGG 0.549078 4_3:+57883515 None:intergenic
GTCTTGGCCAATGAGGTCTC+AGG 0.549623 4_3:+57884170 None:intergenic
CACGGAAAAGGGAGAAAGCA+TGG 0.551213 4_3:-57882875 Msa0650660:CDS
TTGTCCCAACCACGTCCTCA+TGG 0.559642 4_3:-57883524 Msa0650660:CDS
TTCCACATGAGCAGAAAATG+TGG 0.560420 4_3:-57884199 Msa0650660:CDS
AGAAGCAAAAGCAGATACTT+GGG 0.563207 4_3:-57883433 Msa0650660:CDS
TGATGATGCTGGTGCGAATG+AGG 0.565873 4_3:+57883695 None:intergenic
AACGCTGAAAGCAAAAGATA+AGG 0.570182 4_3:-57882769 Msa0650660:CDS
TGCTGCAGCAGCTACCGCTG+TGG 0.578435 4_3:+57884250 None:intergenic
CAAAAGCAGATACTTGGGAA+AGG 0.582056 4_3:-57883428 Msa0650660:CDS
CAATAGATTCATGGGAAAAG+CGG 0.584674 4_3:-57883249 Msa0650660:CDS
TGCAGTATACAAAATCCACT+TGG 0.586711 4_3:-57882568 Msa0650660:three_prime_UTR
CACAGATGAACAAGGAAACA+AGG 0.591861 4_3:-57883766 Msa0650660:CDS
TGGTGGCGGCTGCGGCCATG+AGG 0.604575 4_3:+57883509 None:intergenic
TATATAGACGAGATTGCAGA+AGG 0.610293 4_3:-57882828 Msa0650660:CDS
TTTAAAATCACAGATGAACA+AGG 0.610365 4_3:-57883774 Msa0650660:CDS
GGTCTGACAGGAGGTTGTGG+TGG 0.627760 4_3:+57883477 None:intergenic
CTAGTTGATGGTCTGACAGG+AGG 0.633454 4_3:+57883468 None:intergenic
GTCAGACCATCAACTAGACC+AGG 0.640427 4_3:-57883462 Msa0650660:CDS
TGCAGCAAAGTGAAAATACA+CGG 0.640916 4_3:-57882893 Msa0650660:intron
TCCACATGAGCAGAAAATGT+GGG 0.644028 4_3:-57884198 Msa0650660:CDS
ACGCAAGCTAAACAAGCATG+AGG 0.679702 4_3:-57883209 Msa0650660:intron
TGATGCTGGTGCGAATGAGG+TGG 0.686427 4_3:+57883698 None:intergenic
AACAACAGGAAAGCTTCCAG+GGG 0.710912 4_3:-57882733 Msa0650660:CDS
GGAAATGGAGCATGCCACAG+CGG 0.741947 4_3:-57884264 Msa0650660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATAAATATACTTATAAT+TGG + chr4_3:57884148-57884167 None:intergenic 10.0%
!! TACATAAATATACTTATAAT+TGG + chr4_3:57884148-57884167 None:intergenic 10.0%
!!! AAATGAAGATTTTTTATCAT+AGG + chr4_3:57882692-57882711 None:intergenic 15.0%
!!! AAATGAAGATTTTTTATCAT+AGG + chr4_3:57882692-57882711 None:intergenic 15.0%
!!! TGATTATGATTTTATGGAAA+TGG - chr4_3:57882546-57882565 Msa0650660:three_prime_UTR 20.0%
!!! ATATTTACCTGATAATCTTT+TGG + chr4_3:57882734-57882753 None:intergenic 20.0%
!! GAAAATATAATCAATTTGTC+AGG - chr4_3:57883534-57883553 Msa0650660:CDS 20.0%
!! AGTTTAATCAGCTTATAATT+AGG + chr4_3:57883765-57883784 None:intergenic 20.0%
!! TTTATGTACATAATGTCATT+CGG - chr4_3:57884160-57884179 Msa0650660:CDS 20.0%
!!! TGATTATGATTTTATGGAAA+TGG - chr4_3:57882546-57882565 Msa0650660:three_prime_UTR 20.0%
!!! ATATTTACCTGATAATCTTT+TGG + chr4_3:57882734-57882753 None:intergenic 20.0%
!! GAAAATATAATCAATTTGTC+AGG - chr4_3:57883534-57883553 Msa0650660:CDS 20.0%
!! AGTTTAATCAGCTTATAATT+AGG + chr4_3:57883765-57883784 None:intergenic 20.0%
!! TTTATGTACATAATGTCATT+CGG - chr4_3:57884160-57884179 Msa0650660:CDS 20.0%
! AATTTCTACAGAGAAGAAAA+AGG - chr4_3:57882480-57882499 Msa0650660:three_prime_UTR 25.0%
!! GAGTCATGATTATGATTTTA+TGG - chr4_3:57882540-57882559 Msa0650660:three_prime_UTR 25.0%
!! AAATCTTCATTTTCCCATAT+AGG - chr4_3:57882700-57882719 Msa0650660:exon 25.0%
! TTTAACGTTCTTGTATTCTT+AGG - chr4_3:57882871-57882890 Msa0650660:CDS 25.0%
! TTAACGTTCTTGTATTCTTA+GGG - chr4_3:57882872-57882891 Msa0650660:CDS 25.0%
! TGTATTCTTAGGGATAAATT+CGG - chr4_3:57882882-57882901 Msa0650660:CDS 25.0%
!! TTTAAAATCACAGATGAACA+AGG - chr4_3:57883051-57883070 Msa0650660:intron 25.0%
! CTTCTATTAGAGAAGAAAAA+AGG - chr4_3:57883094-57883113 Msa0650660:intron 25.0%
!!! TTTGATTTTTCACCAAAAGT+AGG + chr4_3:57883168-57883187 None:intergenic 25.0%
! GAGAAATGATGATAAAAAAC+CGG - chr4_3:57883188-57883207 Msa0650660:intron 25.0%
!!! TTGGTTTTTCTTCTTTTCTA+AGG - chr4_3:57884276-57884295 Msa0650660:CDS 25.0%
! AATTTCTACAGAGAAGAAAA+AGG - chr4_3:57882480-57882499 Msa0650660:three_prime_UTR 25.0%
!! GAGTCATGATTATGATTTTA+TGG - chr4_3:57882540-57882559 Msa0650660:three_prime_UTR 25.0%
!! AAATCTTCATTTTCCCATAT+AGG - chr4_3:57882700-57882719 Msa0650660:exon 25.0%
! TTTAACGTTCTTGTATTCTT+AGG - chr4_3:57882871-57882890 Msa0650660:CDS 25.0%
! TTAACGTTCTTGTATTCTTA+GGG - chr4_3:57882872-57882891 Msa0650660:CDS 25.0%
! TGTATTCTTAGGGATAAATT+CGG - chr4_3:57882882-57882901 Msa0650660:CDS 25.0%
!! TTTAAAATCACAGATGAACA+AGG - chr4_3:57883051-57883070 Msa0650660:intron 25.0%
! CTTCTATTAGAGAAGAAAAA+AGG - chr4_3:57883094-57883113 Msa0650660:intron 25.0%
!!! TTTGATTTTTCACCAAAAGT+AGG + chr4_3:57883168-57883187 None:intergenic 25.0%
! GAGAAATGATGATAAAAAAC+CGG - chr4_3:57883188-57883207 Msa0650660:intron 25.0%
!!! TTGGTTTTTCTTCTTTTCTA+AGG - chr4_3:57884276-57884295 Msa0650660:CDS 25.0%
! AGAAGAAAAAGGCACATAAT+TGG - chr4_3:57882491-57882510 Msa0650660:three_prime_UTR 30.0%
AGAACGTTAAAAACTCTTGA+GGG + chr4_3:57882862-57882881 None:intergenic 30.0%
AAGAACGTTAAAAACTCTTG+AGG + chr4_3:57882863-57882882 None:intergenic 30.0%
TCTCTAATAGAAGATGTTGT+TGG + chr4_3:57883087-57883106 None:intergenic 30.0%
! ATGACAAAAACTCCTACTTT+TGG - chr4_3:57883153-57883172 Msa0650660:intron 30.0%
AAATCTTTACCACCAAAACT+TGG + chr4_3:57883237-57883256 None:intergenic 30.0%
!!! TTGGGACAATTTTTGGTTTT+GGG + chr4_3:57883290-57883309 None:intergenic 30.0%
AGCTCAAAAAGATCAAAGAA+AGG - chr4_3:57883424-57883443 Msa0650660:CDS 30.0%
TGTTAGAGACAATAGATTCA+TGG - chr4_3:57883567-57883586 Msa0650660:CDS 30.0%
GTTAGAGACAATAGATTCAT+GGG - chr4_3:57883568-57883587 Msa0650660:CDS 30.0%
! GAGGTTAGATTTTCATATTC+AGG - chr4_3:57883635-57883654 Msa0650660:CDS 30.0%
TTAAAGTGGTTATCCAAACA+GGG + chr4_3:57883661-57883680 None:intergenic 30.0%
ATTAAAGTGGTTATCCAAAC+AGG + chr4_3:57883662-57883681 None:intergenic 30.0%
!!! GAGAATTAACAAGAGCATTT+TGG + chr4_3:57883883-57883902 None:intergenic 30.0%
GTTAATTCTCTGATAAAGTG+TGG - chr4_3:57883893-57883912 Msa0650660:intron 30.0%
AAAGTGAAAATACACGGAAA+AGG - chr4_3:57883938-57883957 Msa0650660:intron 30.0%
AAGTGAAAATACACGGAAAA+GGG - chr4_3:57883939-57883958 Msa0650660:intron 30.0%
GCAAACATTATTAGAACAAC+AGG - chr4_3:57884078-57884097 Msa0650660:intron 30.0%
AGAAAAGAAGAAAAACCAAG+TGG + chr4_3:57884275-57884294 None:intergenic 30.0%
! AGAAGAAAAAGGCACATAAT+TGG - chr4_3:57882491-57882510 Msa0650660:three_prime_UTR 30.0%
AGAACGTTAAAAACTCTTGA+GGG + chr4_3:57882862-57882881 None:intergenic 30.0%
AAGAACGTTAAAAACTCTTG+AGG + chr4_3:57882863-57882882 None:intergenic 30.0%
TCTCTAATAGAAGATGTTGT+TGG + chr4_3:57883087-57883106 None:intergenic 30.0%
! ATGACAAAAACTCCTACTTT+TGG - chr4_3:57883153-57883172 Msa0650660:intron 30.0%
AAATCTTTACCACCAAAACT+TGG + chr4_3:57883237-57883256 None:intergenic 30.0%
!!! TTGGGACAATTTTTGGTTTT+GGG + chr4_3:57883290-57883309 None:intergenic 30.0%
AGCTCAAAAAGATCAAAGAA+AGG - chr4_3:57883424-57883443 Msa0650660:CDS 30.0%
TGTTAGAGACAATAGATTCA+TGG - chr4_3:57883567-57883586 Msa0650660:CDS 30.0%
GTTAGAGACAATAGATTCAT+GGG - chr4_3:57883568-57883587 Msa0650660:CDS 30.0%
! GAGGTTAGATTTTCATATTC+AGG - chr4_3:57883635-57883654 Msa0650660:CDS 30.0%
TTAAAGTGGTTATCCAAACA+GGG + chr4_3:57883661-57883680 None:intergenic 30.0%
ATTAAAGTGGTTATCCAAAC+AGG + chr4_3:57883662-57883681 None:intergenic 30.0%
!!! GAGAATTAACAAGAGCATTT+TGG + chr4_3:57883883-57883902 None:intergenic 30.0%
GTTAATTCTCTGATAAAGTG+TGG - chr4_3:57883893-57883912 Msa0650660:intron 30.0%
AAAGTGAAAATACACGGAAA+AGG - chr4_3:57883938-57883957 Msa0650660:intron 30.0%
AAGTGAAAATACACGGAAAA+GGG - chr4_3:57883939-57883958 Msa0650660:intron 30.0%
GCAAACATTATTAGAACAAC+AGG - chr4_3:57884078-57884097 Msa0650660:intron 30.0%
AGAAAAGAAGAAAAACCAAG+TGG + chr4_3:57884275-57884294 None:intergenic 30.0%
! GGTTTCAGAGACAATTTTCA+AGG - chr4_3:57882512-57882531 Msa0650660:three_prime_UTR 35.0%
TTTATCATAGGCTTTGCTTC+TGG + chr4_3:57882680-57882699 None:intergenic 35.0%
ACTCAATATGCATCACTTGT+AGG - chr4_3:57883024-57883043 Msa0650660:intron 35.0%
!!! GTTTTTGTCATTGATGATGC+TGG + chr4_3:57883144-57883163 None:intergenic 35.0%
!!! CTTTTTGGTGTTAGGATATC+CGG + chr4_3:57883210-57883229 None:intergenic 35.0%
!!! TGGGACAATTTTTGGTTTTG+GGG + chr4_3:57883289-57883308 None:intergenic 35.0%
!!! GTTGGGACAATTTTTGGTTT+TGG + chr4_3:57883291-57883310 None:intergenic 35.0%
AGAAGCAAAAGCAGATACTT+GGG - chr4_3:57883392-57883411 Msa0650660:intron 35.0%
AAAAGCAGATACTTGGGAAA+GGG - chr4_3:57883398-57883417 Msa0650660:intron 35.0%
CAATAGATTCATGGGAAAAG+CGG - chr4_3:57883576-57883595 Msa0650660:CDS 35.0%
TGCAGCAAAGTGAAAATACA+CGG - chr4_3:57883932-57883951 Msa0650660:intron 35.0%
TATATAGACGAGATTGCAGA+AGG - chr4_3:57883997-57884016 Msa0650660:intron 35.0%
TTCATTCTTTCGTCTCTCAT+CGG + chr4_3:57884037-57884056 None:intergenic 35.0%
AACGCTGAAAGCAAAAGATA+AGG - chr4_3:57884056-57884075 Msa0650660:intron 35.0%
GTTCTTTCTTTCTTAGAAGC+AGG + chr4_3:57884124-57884143 None:intergenic 35.0%
TGCAGTATACAAAATCCACT+TGG - chr4_3:57884257-57884276 Msa0650660:CDS 35.0%
! GGTTTCAGAGACAATTTTCA+AGG - chr4_3:57882512-57882531 Msa0650660:three_prime_UTR 35.0%
TTTATCATAGGCTTTGCTTC+TGG + chr4_3:57882680-57882699 None:intergenic 35.0%
ACTCAATATGCATCACTTGT+AGG - chr4_3:57883024-57883043 Msa0650660:intron 35.0%
!!! GTTTTTGTCATTGATGATGC+TGG + chr4_3:57883144-57883163 None:intergenic 35.0%
!!! CTTTTTGGTGTTAGGATATC+CGG + chr4_3:57883210-57883229 None:intergenic 35.0%
!!! TGGGACAATTTTTGGTTTTG+GGG + chr4_3:57883289-57883308 None:intergenic 35.0%
!!! GTTGGGACAATTTTTGGTTT+TGG + chr4_3:57883291-57883310 None:intergenic 35.0%
AGAAGCAAAAGCAGATACTT+GGG - chr4_3:57883392-57883411 Msa0650660:intron 35.0%
AAAAGCAGATACTTGGGAAA+GGG - chr4_3:57883398-57883417 Msa0650660:intron 35.0%
CAATAGATTCATGGGAAAAG+CGG - chr4_3:57883576-57883595 Msa0650660:CDS 35.0%
TGCAGCAAAGTGAAAATACA+CGG - chr4_3:57883932-57883951 Msa0650660:intron 35.0%
TATATAGACGAGATTGCAGA+AGG - chr4_3:57883997-57884016 Msa0650660:intron 35.0%
TTCATTCTTTCGTCTCTCAT+CGG + chr4_3:57884037-57884056 None:intergenic 35.0%
AACGCTGAAAGCAAAAGATA+AGG - chr4_3:57884056-57884075 Msa0650660:intron 35.0%
GTTCTTTCTTTCTTAGAAGC+AGG + chr4_3:57884124-57884143 None:intergenic 35.0%
TGCAGTATACAAAATCCACT+TGG - chr4_3:57884257-57884276 Msa0650660:CDS 35.0%
TTCCACATGAGCAGAAAATG+TGG - chr4_3:57882626-57882645 Msa0650660:three_prime_UTR 40.0%
TCCACATGAGCAGAAAATGT+GGG - chr4_3:57882627-57882646 Msa0650660:three_prime_UTR 40.0%
TTTGGATGCAGCACCTATAT+GGG + chr4_3:57882716-57882735 None:intergenic 40.0%
! TTTTGGATGCAGCACCTATA+TGG + chr4_3:57882717-57882736 None:intergenic 40.0%
GCTGCATCCAAAAGATTATC+AGG - chr4_3:57882724-57882743 Msa0650660:CDS 40.0%
CACAGATGAACAAGGAAACA+AGG - chr4_3:57883059-57883078 Msa0650660:intron 40.0%
CGGATATCCTAACACCAAAA+AGG - chr4_3:57883208-57883227 Msa0650660:intron 40.0%
!!! TTGGAGTCCTTTTTGGTGTT+AGG + chr4_3:57883218-57883237 None:intergenic 40.0%
! CCAAAACTTGGAGTCCTTTT+TGG + chr4_3:57883225-57883244 None:intergenic 40.0%
! CCAAAAAGGACTCCAAGTTT+TGG - chr4_3:57883222-57883241 Msa0650660:CDS 40.0%
!! AAAAGGACTCCAAGTTTTGG+TGG - chr4_3:57883225-57883244 Msa0650660:CDS 40.0%
!!! ATTTTTGGTTTTGGGGTCTC+AGG + chr4_3:57883282-57883301 None:intergenic 40.0%
CAGAAGCAAAAGCAGATACT+TGG - chr4_3:57883391-57883410 Msa0650660:intron 40.0%
CAAAAGCAGATACTTGGGAA+AGG - chr4_3:57883397-57883416 Msa0650660:intron 40.0%
TTTCATATTCAGGCCCTGTT+TGG - chr4_3:57883645-57883664 Msa0650660:CDS 40.0%
GTGTAAGCGCTCAATTAAAG+TGG + chr4_3:57883675-57883694 None:intergenic 40.0%
TTCCACATGAGCAGAAAATG+TGG - chr4_3:57882626-57882645 Msa0650660:three_prime_UTR 40.0%
TCCACATGAGCAGAAAATGT+GGG - chr4_3:57882627-57882646 Msa0650660:three_prime_UTR 40.0%
TTTGGATGCAGCACCTATAT+GGG + chr4_3:57882716-57882735 None:intergenic 40.0%
! TTTTGGATGCAGCACCTATA+TGG + chr4_3:57882717-57882736 None:intergenic 40.0%
GCTGCATCCAAAAGATTATC+AGG - chr4_3:57882724-57882743 Msa0650660:CDS 40.0%
CACAGATGAACAAGGAAACA+AGG - chr4_3:57883059-57883078 Msa0650660:intron 40.0%
CGGATATCCTAACACCAAAA+AGG - chr4_3:57883208-57883227 Msa0650660:intron 40.0%
!!! TTGGAGTCCTTTTTGGTGTT+AGG + chr4_3:57883218-57883237 None:intergenic 40.0%
! CCAAAACTTGGAGTCCTTTT+TGG + chr4_3:57883225-57883244 None:intergenic 40.0%
! CCAAAAAGGACTCCAAGTTT+TGG - chr4_3:57883222-57883241 Msa0650660:CDS 40.0%
!! AAAAGGACTCCAAGTTTTGG+TGG - chr4_3:57883225-57883244 Msa0650660:CDS 40.0%
!!! ATTTTTGGTTTTGGGGTCTC+AGG + chr4_3:57883282-57883301 None:intergenic 40.0%
CAGAAGCAAAAGCAGATACT+TGG - chr4_3:57883391-57883410 Msa0650660:intron 40.0%
CAAAAGCAGATACTTGGGAA+AGG - chr4_3:57883397-57883416 Msa0650660:intron 40.0%
TTTCATATTCAGGCCCTGTT+TGG - chr4_3:57883645-57883664 Msa0650660:CDS 40.0%
GTGTAAGCGCTCAATTAAAG+TGG + chr4_3:57883675-57883694 None:intergenic 40.0%
! TCCCACATTTTCTGCTCATG+TGG + chr4_3:57882631-57882650 None:intergenic 45.0%
ATAGGCTTTGCTTCTGGTCT+TGG + chr4_3:57882674-57882693 None:intergenic 45.0%
CTGAGTAGAACCACAATGTG+CGG - chr4_3:57882827-57882846 Msa0650660:CDS 45.0%
AATTCGGACTCACTACCTCT+TGG - chr4_3:57882898-57882917 Msa0650660:intron 45.0%
CAAGTCTCTTTCAGTCCAAG+AGG + chr4_3:57882916-57882935 None:intergenic 45.0%
! GACGTGGTTGGGACAATTTT+TGG + chr4_3:57883297-57883316 None:intergenic 45.0%
ACGCAAGCTAAACAAGCATG+AGG - chr4_3:57883616-57883635 Msa0650660:CDS 45.0%
AGAACAACAGGAAAGCTTCC+AGG - chr4_3:57884090-57884109 Msa0650660:intron 45.0%
GAACAACAGGAAAGCTTCCA+GGG - chr4_3:57884091-57884110 Msa0650660:intron 45.0%
AACAACAGGAAAGCTTCCAG+GGG - chr4_3:57884092-57884111 Msa0650660:intron 45.0%
! TCCCACATTTTCTGCTCATG+TGG + chr4_3:57882631-57882650 None:intergenic 45.0%
ATAGGCTTTGCTTCTGGTCT+TGG + chr4_3:57882674-57882693 None:intergenic 45.0%
CTGAGTAGAACCACAATGTG+CGG - chr4_3:57882827-57882846 Msa0650660:CDS 45.0%
AATTCGGACTCACTACCTCT+TGG - chr4_3:57882898-57882917 Msa0650660:intron 45.0%
CAAGTCTCTTTCAGTCCAAG+AGG + chr4_3:57882916-57882935 None:intergenic 45.0%
! GACGTGGTTGGGACAATTTT+TGG + chr4_3:57883297-57883316 None:intergenic 45.0%
ACGCAAGCTAAACAAGCATG+AGG - chr4_3:57883616-57883635 Msa0650660:CDS 45.0%
AGAACAACAGGAAAGCTTCC+AGG - chr4_3:57884090-57884109 Msa0650660:intron 45.0%
GAACAACAGGAAAGCTTCCA+GGG - chr4_3:57884091-57884110 Msa0650660:intron 45.0%
AACAACAGGAAAGCTTCCAG+GGG - chr4_3:57884092-57884111 Msa0650660:intron 45.0%
! TGTGGTTCTACTCAGCTCGA+TGG + chr4_3:57882822-57882841 None:intergenic 50.0%
TAGAACCACAATGTGCGGCA+AGG - chr4_3:57882832-57882851 Msa0650660:CDS 50.0%
! GCGAATGAGGTGGTCTTTTC+AGG + chr4_3:57883120-57883139 None:intergenic 50.0%
! TGATGATGCTGGTGCGAATG+AGG + chr4_3:57883133-57883152 None:intergenic 50.0%
!! CTAGTTGATGGTCTGACAGG+AGG + chr4_3:57883360-57883379 None:intergenic 50.0%
!! GGTCTAGTTGATGGTCTGAC+AGG + chr4_3:57883363-57883382 None:intergenic 50.0%
GTCAGACCATCAACTAGACC+AGG - chr4_3:57883363-57883382 Msa0650660:intron 50.0%
GTTGCACCTGGTCTAGTTGA+TGG + chr4_3:57883372-57883391 None:intergenic 50.0%
! GCTTTTGCTTCTGTTGCACC+TGG + chr4_3:57883384-57883403 None:intergenic 50.0%
CACGGAAAAGGGAGAAAGCA+TGG - chr4_3:57883950-57883969 Msa0650660:intron 50.0%
! TGTGGTTCTACTCAGCTCGA+TGG + chr4_3:57882822-57882841 None:intergenic 50.0%
TAGAACCACAATGTGCGGCA+AGG - chr4_3:57882832-57882851 Msa0650660:CDS 50.0%
! GCGAATGAGGTGGTCTTTTC+AGG + chr4_3:57883120-57883139 None:intergenic 50.0%
! TGATGATGCTGGTGCGAATG+AGG + chr4_3:57883133-57883152 None:intergenic 50.0%
!! CTAGTTGATGGTCTGACAGG+AGG + chr4_3:57883360-57883379 None:intergenic 50.0%
!! GGTCTAGTTGATGGTCTGAC+AGG + chr4_3:57883363-57883382 None:intergenic 50.0%
GTCAGACCATCAACTAGACC+AGG - chr4_3:57883363-57883382 Msa0650660:intron 50.0%
GTTGCACCTGGTCTAGTTGA+TGG + chr4_3:57883372-57883391 None:intergenic 50.0%
! GCTTTTGCTTCTGTTGCACC+TGG + chr4_3:57883384-57883403 None:intergenic 50.0%
CACGGAAAAGGGAGAAAGCA+TGG - chr4_3:57883950-57883969 Msa0650660:intron 50.0%
GGAAATGGAGCATGCCACAG+CGG - chr4_3:57882561-57882580 Msa0650660:three_prime_UTR 55.0%
GGAGTTGCCTGAGACCTCAT+TGG - chr4_3:57882648-57882667 Msa0650660:three_prime_UTR 55.0%
GTCTTGGCCAATGAGGTCTC+AGG + chr4_3:57882658-57882677 None:intergenic 55.0%
!! GCTTCTGGTCTTGGCCAATG+AGG + chr4_3:57882665-57882684 None:intergenic 55.0%
TGATGCTGGTGCGAATGAGG+TGG + chr4_3:57883130-57883149 None:intergenic 55.0%
TTGTCCCAACCACGTCCTCA+TGG - chr4_3:57883301-57883320 Msa0650660:intron 55.0%
GATGGTCTGACAGGAGGTTG+TGG + chr4_3:57883354-57883373 None:intergenic 55.0%
TAGAAGCAGGAACAAGCCCC+TGG + chr4_3:57884111-57884130 None:intergenic 55.0%
GGAAATGGAGCATGCCACAG+CGG - chr4_3:57882561-57882580 Msa0650660:three_prime_UTR 55.0%
GGAGTTGCCTGAGACCTCAT+TGG - chr4_3:57882648-57882667 Msa0650660:three_prime_UTR 55.0%
GTCTTGGCCAATGAGGTCTC+AGG + chr4_3:57882658-57882677 None:intergenic 55.0%
!! GCTTCTGGTCTTGGCCAATG+AGG + chr4_3:57882665-57882684 None:intergenic 55.0%
TGATGCTGGTGCGAATGAGG+TGG + chr4_3:57883130-57883149 None:intergenic 55.0%
TTGTCCCAACCACGTCCTCA+TGG - chr4_3:57883301-57883320 Msa0650660:intron 55.0%
GATGGTCTGACAGGAGGTTG+TGG + chr4_3:57883354-57883373 None:intergenic 55.0%
TAGAAGCAGGAACAAGCCCC+TGG + chr4_3:57884111-57884130 None:intergenic 55.0%
GAGAGCCTTGCCGCACATTG+TGG + chr4_3:57882840-57882859 None:intergenic 60.0%
CTGACAGGAGGTTGTGGTGG+CGG + chr4_3:57883348-57883367 None:intergenic 60.0%
GGTCTGACAGGAGGTTGTGG+TGG + chr4_3:57883351-57883370 None:intergenic 60.0%
GAGAGCCTTGCCGCACATTG+TGG + chr4_3:57882840-57882859 None:intergenic 60.0%
CTGACAGGAGGTTGTGGTGG+CGG + chr4_3:57883348-57883367 None:intergenic 60.0%
GGTCTGACAGGAGGTTGTGG+TGG + chr4_3:57883351-57883370 None:intergenic 60.0%
TGCTGCAGCAGCTACCGCTG+TGG + chr4_3:57882578-57882597 None:intergenic 65.0%
GCGGCCATGAGGACGTGGTT+GGG + chr4_3:57883308-57883327 None:intergenic 65.0%
TGCGGCCATGAGGACGTGGT+TGG + chr4_3:57883309-57883328 None:intergenic 65.0%
!! ACAGGAGGTTGTGGTGGCGG+CGG + chr4_3:57883345-57883364 None:intergenic 65.0%
TGCTGCAGCAGCTACCGCTG+TGG + chr4_3:57882578-57882597 None:intergenic 65.0%
GCGGCCATGAGGACGTGGTT+GGG + chr4_3:57883308-57883327 None:intergenic 65.0%
TGCGGCCATGAGGACGTGGT+TGG + chr4_3:57883309-57883328 None:intergenic 65.0%
!! ACAGGAGGTTGTGGTGGCGG+CGG + chr4_3:57883345-57883364 None:intergenic 65.0%
CGGCTGCGGCCATGAGGACG+TGG + chr4_3:57883313-57883332 None:intergenic 75.0%
!! TGGTGGCGGCTGCGGCCATG+AGG + chr4_3:57883319-57883338 None:intergenic 75.0%
!! TGTGGTGGCGGCGGAGGTGG+TGG + chr4_3:57883336-57883355 None:intergenic 75.0%
!! GGTTGTGGTGGCGGCGGAGG+TGG + chr4_3:57883339-57883358 None:intergenic 75.0%
!! GGAGGTTGTGGTGGCGGCGG+AGG + chr4_3:57883342-57883361 None:intergenic 75.0%
CGGCTGCGGCCATGAGGACG+TGG + chr4_3:57883313-57883332 None:intergenic 75.0%
!! TGGTGGCGGCTGCGGCCATG+AGG + chr4_3:57883319-57883338 None:intergenic 75.0%
!! TGTGGTGGCGGCGGAGGTGG+TGG + chr4_3:57883336-57883355 None:intergenic 75.0%
!! GGTTGTGGTGGCGGCGGAGG+TGG + chr4_3:57883339-57883358 None:intergenic 75.0%
!! GGAGGTTGTGGTGGCGGCGG+AGG + chr4_3:57883342-57883361 None:intergenic 75.0%
!!! GGCGGAGGTGGTGGCGGCTG+CGG + chr4_3:57883327-57883346 None:intergenic 80.0%
!! GGTGGCGGCGGAGGTGGTGG+CGG + chr4_3:57883333-57883352 None:intergenic 80.0%
!!! GGCGGAGGTGGTGGCGGCTG+CGG + chr4_3:57883327-57883346 None:intergenic 80.0%
!! GGTGGCGGCGGAGGTGGTGG+CGG + chr4_3:57883333-57883352 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 57882472 57884375 57882472 ID=Msa0650660;Name=Msa0650660
chr4_3 mRNA 57882472 57884375 57882472 ID=Msa0650660-mRNA-1;Parent=Msa0650660;Name=Msa0650660-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.75|1|1|1|4|244|316
chr4_3 exon 57882472 57882910 57882472 ID=Msa0650660-mRNA-1:exon:15169;Parent=Msa0650660-mRNA-1
chr4_3 exon 57883210 57883291 57883210 ID=Msa0650660-mRNA-1:exon:15168;Parent=Msa0650660-mRNA-1
chr4_3 exon 57883402 57883801 57883402 ID=Msa0650660-mRNA-1:exon:15167;Parent=Msa0650660-mRNA-1
chr4_3 exon 57884102 57884375 57884102 ID=Msa0650660-mRNA-1:exon:15166;Parent=Msa0650660-mRNA-1
chr4_3 CDS 57884102 57884375 57884102 ID=Msa0650660-mRNA-1:cds;Parent=Msa0650660-mRNA-1
chr4_3 CDS 57883402 57883801 57883402 ID=Msa0650660-mRNA-1:cds;Parent=Msa0650660-mRNA-1
chr4_3 CDS 57883210 57883291 57883210 ID=Msa0650660-mRNA-1:cds;Parent=Msa0650660-mRNA-1
chr4_3 CDS 57882716 57882910 57882716 ID=Msa0650660-mRNA-1:cds;Parent=Msa0650660-mRNA-1
chr4_3 three_prime_UTR 57882472 57882715 57882472 ID=Msa0650660-mRNA-1:three_prime_utr;Parent=Msa0650660-mRNA-1
Gene Sequence

>Msa0650660

ATGTCTGCAATTTCTACAGAGAAGAAAAAGGCACATAATTGGTTTCAGAGACAATTTTCAAGGAATATGAGTCATGATTATGATTTTATGGAAATGGAGCATGCCACAGCGGTAGCTGCTGCAGCATTTGCAATTAGTTCACATGTCTCAGAAATTCCACATGAGCAGAAAATGTGGGAGTTGCCTGAGACCTCATTGGCCAAGACCAGAAGCAAAGCCTATGATAAAAAATCTTCATTTTCCCATATAGGTGCTGCATCCAAAAGATTATCAGGCTCATTTAAAATCACAGATGAACAAGGAAACAAGGTGCCAACAACATCTTCTATTAGAGAAGAAAAAAGGCCTGAAAAGACCACCTCATTCGCACCAGCATCATCAATGACAAAAACTCCTACTTTTGGTGAAAAATCAAAGAGAAATGATGATAAAAAACCGGATATCCTAACACCAAAAAGGACTCCAAGTTTTGGTGGTAAAGATTTTATTAACACTGATGATATACAACCTGAGACCCCAAAACCAAAAATTGTCCCAACCACGTCCTCATGGCCGCAGCCGCCACCACCTCCGCCGCCACCACAACCTCCTGTCAGACCATCAACTAGACCAGGTGCAACAGAAGCAAAAGCAGATACTTGGGAAAGGGAAGAGCTCAAAAAGATCAAAGAAAGGTATGAGAAGTTGTTAGAGACAATAGATTCATGGGAAAAGCGGAAGAAGATGAAAGCAAGACGCAAGCTAAACAAGCATGAGCAAAGTGAAAATACACGGAAAAGGGAGAAAGCATGGAAAAAATATCAAGATAAGATTAAATATATAGACGAGATTGCAGAAGGAGCAAGAGCACAGTCCGATGAGAGACGAAAGAATGAAACGCTGAAAGCAAAAGATAAGGCAAACATTATTAGAACAACAGGAAAGCTTCCAGGGGCTTGTTCCTGCTTCTAA
Protein sequence

>Msa0650660

MSAISTEKKKAHNWFQRQFSRNMSHDYDFMEMEHATAVAAAAFAISSHVSEIPHEQKMWELPETSLAKTRSKAYDKKSSFSHIGAASKRLSGSFKITDEQGNKVPTTSSIREEKRPEKTTSFAPASSMTKTPTFGEKSKRNDDKKPDILTPKRTPSFGGKDFINTDDIQPETPKPKIVPTTSSWPQPPPPPPPPQPPVRPSTRPGATEAKADTWEREELKKIKERYEKLLETIDSWEKRKKMKARRKLNKHEQSENTRKREKAWKKYQDKIKYIDEIAEGARAQSDERRKNETLKAKDKANIIRTTGKLPGACSCF*