AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0893490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0893490 MtrunA17_Chr4g0067951 97.468 158 4 0 1 158 1 158 2.68e-108 305
Msa0893490 MtrunA17_Chr6g0462311 96.203 158 6 0 1 158 1 158 4.08e-107 302
Msa0893490 MtrunA17_Chr6g0462291 84.049 163 21 1 1 158 1 163 1.48e-92 266
Msa0893490 MtrunA17_Chr2g0295064 74.051 158 41 0 1 158 68 225 2.60e-81 239
Msa0893490 MtrunA17_Chr2g0295021 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0893490 MtrunA17_Chr2g0295001 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0893490 MtrunA17_Chr2g0295051 70.064 157 47 0 1 157 1 157 7.62e-78 228
Msa0893490 MtrunA17_Chr2g0295041 68.354 158 50 0 1 158 1 158 1.47e-75 222
Msa0893490 MtrunA17_Chr2g0295141 63.694 157 57 0 1 157 1 157 1.35e-68 204
Msa0893490 MtrunA17_Chr2g0295104 64.331 157 56 0 1 157 1 157 2.52e-68 204
Msa0893490 MtrunA17_Chr2g0295091 64.331 157 56 0 1 157 1 157 8.50e-68 202
Msa0893490 MtrunA17_Chr2g0295124 64.968 157 55 0 1 157 1 157 8.66e-67 200
Msa0893490 MtrunA17_Chr2g0295081 54.140 157 72 0 1 157 1 157 1.81e-54 169
Msa0893490 MtrunA17_Chr4g0067921 45.963 161 82 4 1 158 1 159 2.51e-37 125
Msa0893490 MtrunA17_Chr2g0295211 37.179 156 97 1 1 155 1 156 2.68e-33 115
Msa0893490 MtrunA17_Chr4g0067841 36.943 157 98 1 1 156 1 157 1.02e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACATAAATATTATT+TGG 0.112159 6_1:-77993175 None:intergenic
CCATGTTTGGTTTGATAATT+TGG 0.145886 6_1:-77992303 None:intergenic
AAAACAACATAAATCTTATT+TGG 0.160723 6_1:-77993145 None:intergenic
CTCCATCAATTGCATCAATT+TGG 0.202998 6_1:-77992672 None:intergenic
TTGATGGCATCAACAGCTTT+TGG 0.227157 6_1:-77992415 None:intergenic
GATATCATTTGAAGCGAAAT+TGG 0.228714 6_1:+77992756 Msa0893490:CDS
TAGATGGATTAGTTGTAATT+AGG 0.259719 6_1:-77992928 None:intergenic
GGCCATTGAGGGTTATGTTT+TGG 0.293726 6_1:+77992900 Msa0893490:CDS
ATTTGGTGATGAAGTTCTAA+TGG 0.299038 6_1:-77992286 None:intergenic
AACGGTTTCAACACTCTTGA+TGG 0.328088 6_1:-77992431 None:intergenic
GGTCCAAATGGAGGGTCTAT+TGG 0.333504 6_1:+77992784 Msa0893490:CDS
TCCTTTGGTTTGATATTTAA+CGG 0.347214 6_1:-77992815 None:intergenic
TGTTGTTAAGTTTATTTACT+TGG 0.354046 6_1:+77993230 Msa0893490:three_prime_UTR
CACCAAATTGATGCAATTGA+TGG 0.359913 6_1:+77992670 Msa0893490:CDS
TAGTAGGTAGACTTGGTAGA+TGG 0.361526 6_1:-77992944 None:intergenic
CTTTGAACAACAACCGGTAA+TGG 0.364496 6_1:-77993284 None:intergenic
GTCCAAATGGAGGGTCTATT+GGG 0.390136 6_1:+77992785 Msa0893490:CDS
AATTGGCTTAGCATCTCCTT+TGG 0.398977 6_1:-77992830 None:intergenic
TATAGTATAGTTGGAGGAGT+AGG 0.400784 6_1:+77992712 Msa0893490:CDS
TGCAATTGATGGAGCAAACT+TGG 0.407405 6_1:+77992681 Msa0893490:CDS
TTTGGTGATGAAGTTCTAAT+GGG 0.409031 6_1:-77992285 None:intergenic
AATCAATAAATTAATACAAA+TGG 0.412812 6_1:+77993113 Msa0893490:three_prime_UTR
TTGAGAGCAAAATCCATTAC+CGG 0.415935 6_1:+77993271 Msa0893490:three_prime_UTR
TTCCCAATAGACCCTCCATT+TGG 0.416216 6_1:-77992787 None:intergenic
AATGTGAATACACCCATGTT+TGG 0.426195 6_1:-77992316 None:intergenic
AGAGCTTTGTAAAGCTTAGC+AGG 0.435032 6_1:-77992367 None:intergenic
AACGAAAGTGAGCTTCTTGA+CGG 0.435771 6_1:-77992476 None:intergenic
ACAGATGATAGTAGATTAGT+AGG 0.436784 6_1:-77992960 None:intergenic
AAGAAGCTCACTTTCGTTGA+GGG 0.454166 6_1:+77992481 Msa0893490:CDS
GGATTAGTTGTAATTAGGAT+TGG 0.464473 6_1:-77992923 None:intergenic
GTGAGCTTCTTGACGGTGCC+AGG 0.467117 6_1:-77992469 None:intergenic
CCAAATTATCAAACCAAACA+TGG 0.473191 6_1:+77992303 Msa0893490:exon
GGTTGAGGAAGGCAAGGCAA+AGG 0.481278 6_1:+77992861 Msa0893490:CDS
GTTGAAACCGTTGAGGGAAA+TGG 0.483004 6_1:+77992442 Msa0893490:CDS
TTTGAAGCGAAATTGGTTGA+AGG 0.487809 6_1:+77992763 Msa0893490:CDS
TTGTTCAAAGCAAAATATCA+AGG 0.488358 6_1:+77993297 Msa0893490:three_prime_UTR
GGGTATAACTATAGTATAGT+TGG 0.489142 6_1:+77992703 Msa0893490:CDS
ATAGTATAGTTGGAGGAGTA+GGG 0.491450 6_1:+77992713 Msa0893490:CDS
AGTAGATTAGTAGGTAGACT+TGG 0.500749 6_1:-77992951 None:intergenic
AGATGCTAAGCCAATTGAAA+AGG 0.515464 6_1:+77992837 Msa0893490:CDS
GTTGAGGGAAATGGTGGTCC+TGG 0.519714 6_1:+77992451 Msa0893490:CDS
GCCAATTGAAAAGGAGGTTG+AGG 0.520188 6_1:+77992846 Msa0893490:CDS
GGCCAAAACATAACCCTCAA+TGG 0.521409 6_1:-77992902 None:intergenic
CAAGAAGCTCACTTTCGTTG+AGG 0.534631 6_1:+77992480 Msa0893490:CDS
GCAATTGATGGAGCAAACTT+GGG 0.541306 6_1:+77992682 Msa0893490:CDS
GTTGAGGAAGGCAAGGCAAA+GGG 0.544771 6_1:+77992862 Msa0893490:CDS
AAGAGTGTTGAAACCGTTGA+GGG 0.546792 6_1:+77992436 Msa0893490:CDS
AATTTGGTGCAACACGTACA+AGG 0.554848 6_1:-77992656 None:intergenic
CAACAAAACACCAAATCTCC+TGG 0.558696 6_1:-77993076 None:intergenic
GGATGATATCATCAGAGTCA+TGG 0.567722 6_1:-77992393 None:intergenic
AAATTGGTTGAAGGTCCAAA+TGG 0.568255 6_1:+77992772 Msa0893490:CDS
AGGACCACCATTTCCCTCAA+CGG 0.572788 6_1:-77992449 None:intergenic
TATAACTATAGTATAGTTGG+AGG 0.584231 6_1:+77992706 Msa0893490:CDS
AGCAGGAGCAACTGTAGAGG+TGG 0.585421 6_1:-77992350 None:intergenic
CTTAGCAGGAGCAACTGTAG+AGG 0.591469 6_1:-77992353 None:intergenic
TGCTAAGCCAATTGAAAAGG+AGG 0.591628 6_1:+77992840 Msa0893490:CDS
ACCGTTAAATATCAAACCAA+AGG 0.596206 6_1:+77992814 Msa0893490:CDS
AGGAGTAGGGTTGCCAGACA+CGG 0.598102 6_1:+77992726 Msa0893490:CDS
TGGTTGAAGGTCCAAATGGA+GGG 0.601542 6_1:+77992776 Msa0893490:CDS
TTGGTTGAAGGTCCAAATGG+AGG 0.609517 6_1:+77992775 Msa0893490:CDS
ATTGAAAAGGAGGTTGAGGA+AGG 0.616285 6_1:+77992850 Msa0893490:CDS
CAAGAGTGTTGAAACCGTTG+AGG 0.622991 6_1:+77992435 Msa0893490:CDS
AAAGGAGGTTGAGGAAGGCA+AGG 0.629508 6_1:+77992855 Msa0893490:CDS
CAATTGATGGAGCAAACTTG+GGG 0.644212 6_1:+77992683 Msa0893490:CDS
CAAATTATCAAACCAAACAT+GGG 0.651382 6_1:+77992304 Msa0893490:exon
CCATATGCTCATAAGCCACA+CGG 0.666552 6_1:+77992210 None:intergenic
GAAACCGTTGAGGGAAATGG+TGG 0.710996 6_1:+77992445 Msa0893490:CDS
TGTATGTTAGTGTGTCCGTG+TGG 0.751770 6_1:-77992225 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATAAATTAATACAAA+TGG + chr6_1:77993113-77993132 Msa0893490:three_prime_UTR 10.0%
!! AATCAATAAATTAATACAAA+TGG + chr6_1:77993113-77993132 Msa0893490:three_prime_UTR 10.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr6_1:77993031-77993050 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr6_1:77993035-77993054 Msa0893490:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr6_1:77993148-77993167 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr6_1:77993178-77993197 None:intergenic 15.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr6_1:77993031-77993050 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr6_1:77993035-77993054 Msa0893490:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr6_1:77993148-77993167 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr6_1:77993178-77993197 None:intergenic 15.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr6_1:77993230-77993249 Msa0893490:three_prime_UTR 20.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr6_1:77993230-77993249 Msa0893490:three_prime_UTR 20.0%
! CAAATTATCAAACCAAACAT+GGG + chr6_1:77992304-77992323 Msa0893490:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr6_1:77992706-77992725 Msa0893490:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr6_1:77992818-77992837 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr6_1:77992931-77992950 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG + chr6_1:77993297-77993316 Msa0893490:three_prime_UTR 25.0%
! CAAATTATCAAACCAAACAT+GGG + chr6_1:77992304-77992323 Msa0893490:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr6_1:77992706-77992725 Msa0893490:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr6_1:77992818-77992837 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr6_1:77992931-77992950 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG + chr6_1:77993297-77993316 Msa0893490:three_prime_UTR 25.0%
!! TTTGGTGATGAAGTTCTAAT+GGG - chr6_1:77992288-77992307 None:intergenic 30.0%
!! ATTTGGTGATGAAGTTCTAA+TGG - chr6_1:77992289-77992308 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr6_1:77992306-77992325 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr6_1:77992303-77992322 Msa0893490:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr6_1:77992563-77992582 Msa0893490:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr6_1:77992703-77992722 Msa0893490:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr6_1:77992756-77992775 Msa0893490:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr6_1:77992814-77992833 Msa0893490:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr6_1:77992926-77992945 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr6_1:77992963-77992982 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr6_1:77993058-77993077 Msa0893490:three_prime_UTR 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG - chr6_1:77993293-77993312 None:intergenic 30.0%
!! TTTGGTGATGAAGTTCTAAT+GGG - chr6_1:77992288-77992307 None:intergenic 30.0%
!! ATTTGGTGATGAAGTTCTAA+TGG - chr6_1:77992289-77992308 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr6_1:77992306-77992325 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr6_1:77992303-77992322 Msa0893490:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr6_1:77992563-77992582 Msa0893490:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr6_1:77992703-77992722 Msa0893490:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr6_1:77992756-77992775 Msa0893490:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr6_1:77992814-77992833 Msa0893490:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr6_1:77992926-77992945 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr6_1:77992963-77992982 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr6_1:77993058-77993077 Msa0893490:three_prime_UTR 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG - chr6_1:77993293-77993312 None:intergenic 30.0%
AATGTGAATACACCCATGTT+TGG - chr6_1:77992319-77992338 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr6_1:77992625-77992644 Msa0893490:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr6_1:77992629-77992648 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr6_1:77992628-77992647 Msa0893490:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr6_1:77992629-77992648 Msa0893490:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr6_1:77992670-77992689 Msa0893490:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr6_1:77992675-77992694 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr6_1:77992712-77992731 Msa0893490:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr6_1:77992713-77992732 Msa0893490:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr6_1:77992763-77992782 Msa0893490:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr6_1:77992772-77992791 Msa0893490:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr6_1:77992837-77992856 Msa0893490:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr6_1:77992954-77992973 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG + chr6_1:77993271-77993290 Msa0893490:three_prime_UTR 35.0%
AATGTGAATACACCCATGTT+TGG - chr6_1:77992319-77992338 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr6_1:77992625-77992644 Msa0893490:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr6_1:77992629-77992648 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr6_1:77992628-77992647 Msa0893490:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr6_1:77992629-77992648 Msa0893490:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr6_1:77992670-77992689 Msa0893490:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr6_1:77992675-77992694 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr6_1:77992712-77992731 Msa0893490:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr6_1:77992713-77992732 Msa0893490:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr6_1:77992763-77992782 Msa0893490:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr6_1:77992772-77992791 Msa0893490:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr6_1:77992837-77992856 Msa0893490:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr6_1:77992954-77992973 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG + chr6_1:77993271-77993290 Msa0893490:three_prime_UTR 35.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr6_1:77992370-77992389 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr6_1:77992396-77992415 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr6_1:77992417-77992436 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr6_1:77992418-77992437 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr6_1:77992434-77992453 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr6_1:77992436-77992455 Msa0893490:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr6_1:77992479-77992498 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr6_1:77992481-77992500 Msa0893490:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr6_1:77992659-77992678 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr6_1:77992681-77992700 Msa0893490:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr6_1:77992682-77992701 Msa0893490:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr6_1:77992683-77992702 Msa0893490:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr6_1:77992742-77992761 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr6_1:77992833-77992852 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr6_1:77992840-77992859 Msa0893490:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr6_1:77992850-77992869 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr6_1:77992850-77992869 Msa0893490:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr6_1:77992879-77992898 Msa0893490:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr6_1:77992947-77992966 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr6_1:77993066-77993085 Msa0893490:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr6_1:77993079-77993098 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG - chr6_1:77993287-77993306 None:intergenic 40.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr6_1:77992370-77992389 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr6_1:77992396-77992415 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr6_1:77992417-77992436 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr6_1:77992418-77992437 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr6_1:77992434-77992453 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr6_1:77992436-77992455 Msa0893490:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr6_1:77992479-77992498 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr6_1:77992481-77992500 Msa0893490:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr6_1:77992659-77992678 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr6_1:77992681-77992700 Msa0893490:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr6_1:77992682-77992701 Msa0893490:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr6_1:77992683-77992702 Msa0893490:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr6_1:77992742-77992761 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr6_1:77992833-77992852 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr6_1:77992840-77992859 Msa0893490:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr6_1:77992850-77992869 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr6_1:77992850-77992869 Msa0893490:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr6_1:77992879-77992898 Msa0893490:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr6_1:77992947-77992966 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr6_1:77993066-77993085 Msa0893490:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr6_1:77993079-77993098 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG - chr6_1:77993287-77993306 None:intergenic 40.0%
TGTATGTTAGTGTGTCCGTG+TGG - chr6_1:77992228-77992247 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr6_1:77992435-77992454 Msa0893490:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr6_1:77992442-77992461 Msa0893490:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr6_1:77992480-77992499 Msa0893490:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr6_1:77992775-77992794 Msa0893490:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr6_1:77992776-77992795 Msa0893490:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr6_1:77992785-77992804 Msa0893490:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr6_1:77992790-77992809 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr6_1:77992846-77992865 Msa0893490:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr6_1:77992888-77992907 Msa0893490:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr6_1:77992889-77992908 Msa0893490:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr6_1:77992900-77992919 Msa0893490:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr6_1:77992905-77992924 None:intergenic 45.0%
TGTATGTTAGTGTGTCCGTG+TGG - chr6_1:77992228-77992247 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr6_1:77992435-77992454 Msa0893490:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr6_1:77992442-77992461 Msa0893490:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr6_1:77992480-77992499 Msa0893490:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr6_1:77992775-77992794 Msa0893490:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr6_1:77992776-77992795 Msa0893490:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr6_1:77992785-77992804 Msa0893490:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr6_1:77992790-77992809 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr6_1:77992846-77992865 Msa0893490:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr6_1:77992888-77992907 Msa0893490:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr6_1:77992889-77992908 Msa0893490:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr6_1:77992900-77992919 Msa0893490:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr6_1:77992905-77992924 None:intergenic 45.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr6_1:77992356-77992375 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr6_1:77992445-77992464 Msa0893490:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr6_1:77992452-77992471 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr6_1:77992784-77992803 Msa0893490:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr6_1:77992855-77992874 Msa0893490:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr6_1:77992862-77992881 Msa0893490:CDS 50.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr6_1:77992356-77992375 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr6_1:77992445-77992464 Msa0893490:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr6_1:77992452-77992471 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr6_1:77992784-77992803 Msa0893490:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr6_1:77992855-77992874 Msa0893490:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr6_1:77992862-77992881 Msa0893490:CDS 50.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr6_1:77992353-77992372 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr6_1:77992451-77992470 Msa0893490:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr6_1:77992726-77992745 Msa0893490:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr6_1:77992861-77992880 Msa0893490:CDS 55.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr6_1:77992353-77992372 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr6_1:77992451-77992470 Msa0893490:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr6_1:77992726-77992745 Msa0893490:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr6_1:77992861-77992880 Msa0893490:CDS 55.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr6_1:77992472-77992491 None:intergenic 60.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr6_1:77992472-77992491 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 77992222 77993323 77992222 ID=Msa0893490;Name=Msa0893490
chr6_1 mRNA 77992222 77993323 77992222 ID=Msa0893490-mRNA-1;Parent=Msa0893490;Name=Msa0893490-mRNA-1;_AED=0.07;_eAED=0.07;_QI=100|1|1|1|1|1|2|381|158
chr6_1 exon 77992222 77992502 77992222 ID=Msa0893490-mRNA-1:exon:12905;Parent=Msa0893490-mRNA-1
chr6_1 exon 77992647 77993323 77992647 ID=Msa0893490-mRNA-1:exon:12906;Parent=Msa0893490-mRNA-1
chr6_1 five_prime_UTR 77992222 77992321 77992222 ID=Msa0893490-mRNA-1:five_prime_utr;Parent=Msa0893490-mRNA-1
chr6_1 CDS 77992322 77992502 77992322 ID=Msa0893490-mRNA-1:cds;Parent=Msa0893490-mRNA-1
chr6_1 CDS 77992647 77992942 77992647 ID=Msa0893490-mRNA-1:cds;Parent=Msa0893490-mRNA-1
chr6_1 three_prime_UTR 77992943 77993323 77992943 ID=Msa0893490-mRNA-1:three_prime_utr;Parent=Msa0893490-mRNA-1
Gene Sequence

>Msa0893490

ATGGGTGTATTCACATTTGAGCAAGAAACCACCTCTACAGTTGCTCCTGCTAAGCTTTACAAAGCTCTTGTCCATGACTCTGATGATATCATCCCAAAAGCTGTTGATGCCATCAAGAGTGTTGAAACCGTTGAGGGAAATGGTGGTCCTGGCACCGTCAAGAAGCTCACTTTCGTTGAGGGAGGGCAAACCTTGTACGTGTTGCACCAAATTGATGCAATTGATGGAGCAAACTTGGGGTATAACTATAGTATAGTTGGAGGAGTAGGGTTGCCAGACACGGTTGAAAAGATATCATTTGAAGCGAAATTGGTTGAAGGTCCAAATGGAGGGTCTATTGGGAAGACGACCGTTAAATATCAAACCAAAGGAGATGCTAAGCCAATTGAAAAGGAGGTTGAGGAAGGCAAGGCAAAGGGTGATGCTCTTTTCAAGGCCATTGAGGGTTATGTTTTGGCCAATCCTAATTACAACTAA
Protein sequence

>Msa0893490

MGVFTFEQETTSTVAPAKLYKALVHDSDDIIPKAVDAIKSVETVEGNGGPGTVKKLTFVEGGQTLYVLHQIDAIDGANLGYNYSIVGGVGLPDTVEKISFEAKLVEGPNGGSIGKTTVKYQTKGDAKPIEKEVEEGKAKGDALFKAIEGYVLANPNYN*