AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0138850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138850 MtrunA17_Chr4g0067951 97.468 158 4 0 1 158 1 158 2.68e-108 305
Msa0138850 MtrunA17_Chr6g0462311 96.203 158 6 0 1 158 1 158 4.08e-107 302
Msa0138850 MtrunA17_Chr6g0462291 84.049 163 21 1 1 158 1 163 1.48e-92 266
Msa0138850 MtrunA17_Chr2g0295064 74.051 158 41 0 1 158 68 225 2.60e-81 239
Msa0138850 MtrunA17_Chr2g0295021 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0138850 MtrunA17_Chr2g0295001 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0138850 MtrunA17_Chr2g0295051 70.064 157 47 0 1 157 1 157 7.62e-78 228
Msa0138850 MtrunA17_Chr2g0295041 68.354 158 50 0 1 158 1 158 1.47e-75 222
Msa0138850 MtrunA17_Chr2g0295141 63.694 157 57 0 1 157 1 157 1.35e-68 204
Msa0138850 MtrunA17_Chr2g0295104 64.331 157 56 0 1 157 1 157 2.52e-68 204
Msa0138850 MtrunA17_Chr2g0295091 64.331 157 56 0 1 157 1 157 8.50e-68 202
Msa0138850 MtrunA17_Chr2g0295124 64.968 157 55 0 1 157 1 157 8.66e-67 200
Msa0138850 MtrunA17_Chr2g0295081 54.140 157 72 0 1 157 1 157 1.81e-54 169
Msa0138850 MtrunA17_Chr4g0067921 45.963 161 82 4 1 158 1 159 2.51e-37 125
Msa0138850 MtrunA17_Chr2g0295211 37.179 156 97 1 1 155 1 156 2.68e-33 115
Msa0138850 MtrunA17_Chr4g0067841 36.943 157 98 1 1 156 1 157 1.02e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACATAAATATTATT+TGG 0.112159 1_3:-72073229 None:intergenic
CCATGTTTGGTTTGATAATT+TGG 0.145886 1_3:-72072357 None:intergenic
AAAACAACATAAATCTTATT+TGG 0.160723 1_3:-72073199 None:intergenic
TTTGGTGATGAAGTTGTAAA+AGG 0.190525 1_3:-72072339 None:intergenic
CTCCATCAATTGCATCAATT+TGG 0.202998 1_3:-72072726 None:intergenic
TTGATGGCATCAACAGCTTT+TGG 0.227157 1_3:-72072469 None:intergenic
GATATCATTTGAAGCGAAAT+TGG 0.228714 1_3:+72072810 Msa0138850:CDS
TAGATGGATTAGTTGTAATT+AGG 0.259719 1_3:-72072982 None:intergenic
GGCCATTGAGGGTTATGTTT+TGG 0.293726 1_3:+72072954 Msa0138850:CDS
AACGGTTTCAACACTCTTGA+TGG 0.328088 1_3:-72072485 None:intergenic
GGTCCAAATGGAGGGTCTAT+TGG 0.333504 1_3:+72072838 Msa0138850:CDS
TCCTTTGGTTTGATATTTAA+CGG 0.347214 1_3:-72072869 None:intergenic
TGTTGTTAAGTTTATTTACT+TGG 0.354046 1_3:+72073284 Msa0138850:three_prime_UTR
CACCAAATTGATGCAATTGA+TGG 0.359913 1_3:+72072724 Msa0138850:CDS
TAGTAGGTAGACTTGGTAGA+TGG 0.361526 1_3:-72072998 None:intergenic
GTCCAAATGGAGGGTCTATT+GGG 0.390136 1_3:+72072839 Msa0138850:CDS
AATTGGCTTAGCATCTCCTT+TGG 0.398977 1_3:-72072884 None:intergenic
TATAGTATAGTTGGAGGAGT+AGG 0.400784 1_3:+72072766 Msa0138850:CDS
TGCAATTGATGGAGCAAACT+TGG 0.407405 1_3:+72072735 Msa0138850:CDS
AATCAATAAATTAATACAAA+TGG 0.412812 1_3:+72073167 Msa0138850:three_prime_UTR
TTCCCAATAGACCCTCCATT+TGG 0.416216 1_3:-72072841 None:intergenic
AATGTGAATACACCCATGTT+TGG 0.426195 1_3:-72072370 None:intergenic
AGAGCTTTGTAAAGCTTAGC+AGG 0.435032 1_3:-72072421 None:intergenic
AACGAAAGTGAGCTTCTTGA+CGG 0.435771 1_3:-72072530 None:intergenic
ACAGATGATAGTAGATTAGT+AGG 0.436784 1_3:-72073014 None:intergenic
TTGGTGATGAAGTTGTAAAA+GGG 0.438115 1_3:-72072338 None:intergenic
AAGAAGCTCACTTTCGTTGA+GGG 0.454166 1_3:+72072535 Msa0138850:CDS
GGATTAGTTGTAATTAGGAT+TGG 0.464473 1_3:-72072977 None:intergenic
GTGAGCTTCTTGACGGTGCC+AGG 0.467117 1_3:-72072523 None:intergenic
CCAAATTATCAAACCAAACA+TGG 0.473191 1_3:+72072357 Msa0138850:exon
GGTTGAGGAAGGCAAGGCAA+AGG 0.481278 1_3:+72072915 Msa0138850:CDS
GTTGAAACCGTTGAGGGAAA+TGG 0.483004 1_3:+72072496 Msa0138850:CDS
TATGTATGTTGGTGTGTGGC+TGG 0.486530 1_3:-72072277 None:intergenic
TTTGAAGCGAAATTGGTTGA+AGG 0.487809 1_3:+72072817 Msa0138850:CDS
GGGTATAACTATAGTATAGT+TGG 0.489142 1_3:+72072757 Msa0138850:CDS
ATAGTATAGTTGGAGGAGTA+GGG 0.491450 1_3:+72072767 Msa0138850:CDS
AGTAGATTAGTAGGTAGACT+TGG 0.500749 1_3:-72073005 None:intergenic
AGATGCTAAGCCAATTGAAA+AGG 0.515464 1_3:+72072891 Msa0138850:CDS
GTTGAGGGAAATGGTGGTCC+TGG 0.519714 1_3:+72072505 Msa0138850:CDS
GCCAATTGAAAAGGAGGTTG+AGG 0.520188 1_3:+72072900 Msa0138850:CDS
GGCCAAAACATAACCCTCAA+TGG 0.521409 1_3:-72072956 None:intergenic
CAAGAAGCTCACTTTCGTTG+AGG 0.534631 1_3:+72072534 Msa0138850:CDS
GCAATTGATGGAGCAAACTT+GGG 0.541306 1_3:+72072736 Msa0138850:CDS
GTTGAGGAAGGCAAGGCAAA+GGG 0.544771 1_3:+72072916 Msa0138850:CDS
AAGAGTGTTGAAACCGTTGA+GGG 0.546792 1_3:+72072490 Msa0138850:CDS
AATTTGGTGCAACACGTACA+AGG 0.554848 1_3:-72072710 None:intergenic
TGAGAACAAGATATGTATGT+TGG 0.557908 1_3:-72072288 None:intergenic
CAACAAAACACCAAATCTCC+TGG 0.558696 1_3:-72073130 None:intergenic
GGATGATATCATCAGAGTCA+TGG 0.567722 1_3:-72072447 None:intergenic
AAATTGGTTGAAGGTCCAAA+TGG 0.568255 1_3:+72072826 Msa0138850:CDS
AGGACCACCATTTCCCTCAA+CGG 0.572788 1_3:-72072503 None:intergenic
TATAACTATAGTATAGTTGG+AGG 0.584231 1_3:+72072760 Msa0138850:CDS
AGCAGGAGCAACTGTAGAGG+TGG 0.585421 1_3:-72072404 None:intergenic
CTTAGCAGGAGCAACTGTAG+AGG 0.591469 1_3:-72072407 None:intergenic
TGCTAAGCCAATTGAAAAGG+AGG 0.591628 1_3:+72072894 Msa0138850:CDS
ACCGTTAAATATCAAACCAA+AGG 0.596206 1_3:+72072868 Msa0138850:CDS
AGGAGTAGGGTTGCCAGACA+CGG 0.598102 1_3:+72072780 Msa0138850:CDS
TGGTTGAAGGTCCAAATGGA+GGG 0.601542 1_3:+72072830 Msa0138850:CDS
TTGGTTGAAGGTCCAAATGG+AGG 0.609517 1_3:+72072829 Msa0138850:CDS
ATTGAAAAGGAGGTTGAGGA+AGG 0.616285 1_3:+72072904 Msa0138850:CDS
CAAGAGTGTTGAAACCGTTG+AGG 0.622991 1_3:+72072489 Msa0138850:CDS
AAAGGAGGTTGAGGAAGGCA+AGG 0.629508 1_3:+72072909 Msa0138850:CDS
CAATTGATGGAGCAAACTTG+GGG 0.644212 1_3:+72072737 Msa0138850:CDS
CAAATTATCAAACCAAACAT+GGG 0.651382 1_3:+72072358 Msa0138850:exon
AAGATATGTATGTTGGTGTG+TGG 0.694182 1_3:-72072281 None:intergenic
GAAACCGTTGAGGGAAATGG+TGG 0.710996 1_3:+72072499 Msa0138850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATAAATTAATACAAA+TGG + chr1_3:72073167-72073186 Msa0138850:three_prime_UTR 10.0%
!! AATCAATAAATTAATACAAA+TGG + chr1_3:72073167-72073186 Msa0138850:three_prime_UTR 10.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr1_3:72073085-72073104 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr1_3:72073089-72073108 Msa0138850:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr1_3:72073202-72073221 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr1_3:72073232-72073251 None:intergenic 15.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr1_3:72073085-72073104 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr1_3:72073089-72073108 Msa0138850:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr1_3:72073202-72073221 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr1_3:72073232-72073251 None:intergenic 15.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr1_3:72073284-72073303 Msa0138850:three_prime_UTR 20.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr1_3:72073284-72073303 Msa0138850:three_prime_UTR 20.0%
! CAAATTATCAAACCAAACAT+GGG + chr1_3:72072358-72072377 Msa0138850:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr1_3:72072760-72072779 Msa0138850:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr1_3:72072872-72072891 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr1_3:72072985-72073004 None:intergenic 25.0%
! CAAATTATCAAACCAAACAT+GGG + chr1_3:72072358-72072377 Msa0138850:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr1_3:72072760-72072779 Msa0138850:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr1_3:72072872-72072891 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr1_3:72072985-72073004 None:intergenic 25.0%
TGAGAACAAGATATGTATGT+TGG - chr1_3:72072291-72072310 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG - chr1_3:72072341-72072360 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG - chr1_3:72072342-72072361 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr1_3:72072360-72072379 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr1_3:72072357-72072376 Msa0138850:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr1_3:72072617-72072636 Msa0138850:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr1_3:72072757-72072776 Msa0138850:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr1_3:72072810-72072829 Msa0138850:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr1_3:72072868-72072887 Msa0138850:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr1_3:72072980-72072999 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr1_3:72073017-72073036 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr1_3:72073112-72073131 Msa0138850:three_prime_UTR 30.0%
TGAGAACAAGATATGTATGT+TGG - chr1_3:72072291-72072310 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG - chr1_3:72072341-72072360 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG - chr1_3:72072342-72072361 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr1_3:72072360-72072379 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr1_3:72072357-72072376 Msa0138850:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr1_3:72072617-72072636 Msa0138850:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr1_3:72072757-72072776 Msa0138850:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr1_3:72072810-72072829 Msa0138850:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr1_3:72072868-72072887 Msa0138850:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr1_3:72072980-72072999 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr1_3:72073017-72073036 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr1_3:72073112-72073131 Msa0138850:three_prime_UTR 30.0%
AAGATATGTATGTTGGTGTG+TGG - chr1_3:72072284-72072303 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG - chr1_3:72072373-72072392 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr1_3:72072679-72072698 Msa0138850:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr1_3:72072683-72072702 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr1_3:72072682-72072701 Msa0138850:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr1_3:72072683-72072702 Msa0138850:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr1_3:72072724-72072743 Msa0138850:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr1_3:72072729-72072748 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr1_3:72072766-72072785 Msa0138850:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr1_3:72072767-72072786 Msa0138850:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr1_3:72072817-72072836 Msa0138850:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr1_3:72072826-72072845 Msa0138850:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr1_3:72072891-72072910 Msa0138850:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr1_3:72073008-72073027 None:intergenic 35.0%
AAGATATGTATGTTGGTGTG+TGG - chr1_3:72072284-72072303 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG - chr1_3:72072373-72072392 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr1_3:72072679-72072698 Msa0138850:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr1_3:72072683-72072702 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr1_3:72072682-72072701 Msa0138850:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr1_3:72072683-72072702 Msa0138850:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr1_3:72072724-72072743 Msa0138850:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr1_3:72072729-72072748 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr1_3:72072766-72072785 Msa0138850:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr1_3:72072767-72072786 Msa0138850:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr1_3:72072817-72072836 Msa0138850:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr1_3:72072826-72072845 Msa0138850:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr1_3:72072891-72072910 Msa0138850:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr1_3:72073008-72073027 None:intergenic 35.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr1_3:72072424-72072443 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr1_3:72072450-72072469 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr1_3:72072471-72072490 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr1_3:72072472-72072491 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr1_3:72072488-72072507 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr1_3:72072490-72072509 Msa0138850:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr1_3:72072533-72072552 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr1_3:72072535-72072554 Msa0138850:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr1_3:72072713-72072732 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr1_3:72072735-72072754 Msa0138850:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr1_3:72072736-72072755 Msa0138850:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr1_3:72072737-72072756 Msa0138850:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr1_3:72072796-72072815 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr1_3:72072887-72072906 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr1_3:72072894-72072913 Msa0138850:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr1_3:72072904-72072923 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr1_3:72072904-72072923 Msa0138850:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr1_3:72072933-72072952 Msa0138850:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr1_3:72073001-72073020 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr1_3:72073120-72073139 Msa0138850:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr1_3:72073133-72073152 None:intergenic 40.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr1_3:72072424-72072443 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr1_3:72072450-72072469 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr1_3:72072471-72072490 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr1_3:72072472-72072491 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr1_3:72072488-72072507 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr1_3:72072490-72072509 Msa0138850:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr1_3:72072533-72072552 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr1_3:72072535-72072554 Msa0138850:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr1_3:72072713-72072732 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr1_3:72072735-72072754 Msa0138850:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr1_3:72072736-72072755 Msa0138850:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr1_3:72072737-72072756 Msa0138850:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr1_3:72072796-72072815 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr1_3:72072887-72072906 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr1_3:72072894-72072913 Msa0138850:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr1_3:72072904-72072923 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr1_3:72072904-72072923 Msa0138850:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr1_3:72072933-72072952 Msa0138850:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr1_3:72073001-72073020 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr1_3:72073120-72073139 Msa0138850:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr1_3:72073133-72073152 None:intergenic 40.0%
TATGTATGTTGGTGTGTGGC+TGG - chr1_3:72072280-72072299 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr1_3:72072489-72072508 Msa0138850:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr1_3:72072496-72072515 Msa0138850:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr1_3:72072534-72072553 Msa0138850:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr1_3:72072829-72072848 Msa0138850:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr1_3:72072830-72072849 Msa0138850:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr1_3:72072839-72072858 Msa0138850:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr1_3:72072844-72072863 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr1_3:72072900-72072919 Msa0138850:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr1_3:72072942-72072961 Msa0138850:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr1_3:72072943-72072962 Msa0138850:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr1_3:72072954-72072973 Msa0138850:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr1_3:72072959-72072978 None:intergenic 45.0%
TATGTATGTTGGTGTGTGGC+TGG - chr1_3:72072280-72072299 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr1_3:72072489-72072508 Msa0138850:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr1_3:72072496-72072515 Msa0138850:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr1_3:72072534-72072553 Msa0138850:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr1_3:72072829-72072848 Msa0138850:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr1_3:72072830-72072849 Msa0138850:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr1_3:72072839-72072858 Msa0138850:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr1_3:72072844-72072863 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr1_3:72072900-72072919 Msa0138850:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr1_3:72072942-72072961 Msa0138850:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr1_3:72072943-72072962 Msa0138850:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr1_3:72072954-72072973 Msa0138850:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr1_3:72072959-72072978 None:intergenic 45.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr1_3:72072410-72072429 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr1_3:72072499-72072518 Msa0138850:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr1_3:72072506-72072525 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr1_3:72072838-72072857 Msa0138850:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr1_3:72072909-72072928 Msa0138850:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr1_3:72072916-72072935 Msa0138850:CDS 50.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr1_3:72072410-72072429 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr1_3:72072499-72072518 Msa0138850:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr1_3:72072506-72072525 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr1_3:72072838-72072857 Msa0138850:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr1_3:72072909-72072928 Msa0138850:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr1_3:72072916-72072935 Msa0138850:CDS 50.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr1_3:72072407-72072426 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr1_3:72072505-72072524 Msa0138850:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr1_3:72072780-72072799 Msa0138850:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr1_3:72072915-72072934 Msa0138850:CDS 55.0%
! ATGTTGGTGTGTGGCTGGCT+TGG - chr1_3:72072275-72072294 None:intergenic 55.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr1_3:72072407-72072426 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr1_3:72072505-72072524 Msa0138850:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr1_3:72072780-72072799 Msa0138850:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr1_3:72072915-72072934 Msa0138850:CDS 55.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr1_3:72072526-72072545 None:intergenic 60.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr1_3:72072526-72072545 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 72072274 72073339 72072274 ID=Msa0138850;Name=Msa0138850
chr1_3 mRNA 72072274 72073339 72072274 ID=Msa0138850-mRNA-1;Parent=Msa0138850;Name=Msa0138850-mRNA-1;_AED=0.39;_eAED=0.39;_QI=102|1|1|1|1|1|2|343|158
chr1_3 exon 72072274 72072556 72072274 ID=Msa0138850-mRNA-1:exon:25113;Parent=Msa0138850-mRNA-1
chr1_3 exon 72072701 72073339 72072701 ID=Msa0138850-mRNA-1:exon:25114;Parent=Msa0138850-mRNA-1
chr1_3 five_prime_UTR 72072274 72072375 72072274 ID=Msa0138850-mRNA-1:five_prime_utr;Parent=Msa0138850-mRNA-1
chr1_3 CDS 72072376 72072556 72072376 ID=Msa0138850-mRNA-1:cds;Parent=Msa0138850-mRNA-1
chr1_3 CDS 72072701 72072996 72072701 ID=Msa0138850-mRNA-1:cds;Parent=Msa0138850-mRNA-1
chr1_3 three_prime_UTR 72072997 72073339 72072997 ID=Msa0138850-mRNA-1:three_prime_utr;Parent=Msa0138850-mRNA-1
Gene Sequence

>Msa0138850

ATGGGTGTATTCACATTTGAGCAAGAAACCACCTCTACAGTTGCTCCTGCTAAGCTTTACAAAGCTCTTGTCCATGACTCTGATGATATCATCCCAAAAGCTGTTGATGCCATCAAGAGTGTTGAAACCGTTGAGGGAAATGGTGGTCCTGGCACCGTCAAGAAGCTCACTTTCGTTGAGGGAGGGCAAACCTTGTACGTGTTGCACCAAATTGATGCAATTGATGGAGCAAACTTGGGGTATAACTATAGTATAGTTGGAGGAGTAGGGTTGCCAGACACGGTTGAAAAGATATCATTTGAAGCGAAATTGGTTGAAGGTCCAAATGGAGGGTCTATTGGGAAGACGACCGTTAAATATCAAACCAAAGGAGATGCTAAGCCAATTGAAAAGGAGGTTGAGGAAGGCAAGGCAAAGGGTGATGCTCTTTTCAAGGCCATTGAGGGTTATGTTTTGGCCAATCCTAATTACAACTAA
Protein sequence

>Msa0138850

MGVFTFEQETTSTVAPAKLYKALVHDSDDIIPKAVDAIKSVETVEGNGGPGTVKKLTFVEGGQTLYVLHQIDAIDGANLGYNYSIVGGVGLPDTVEKISFEAKLVEGPNGGSIGKTTVKYQTKGDAKPIEKEVEEGKAKGDALFKAIEGYVLANPNYN*