AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218200 MtrunA17_Chr4g0067951 97.468 158 4 0 1 158 1 158 2.68e-108 305
Msa1218200 MtrunA17_Chr6g0462311 96.203 158 6 0 1 158 1 158 4.08e-107 302
Msa1218200 MtrunA17_Chr6g0462291 84.049 163 21 1 1 158 1 163 1.48e-92 266
Msa1218200 MtrunA17_Chr2g0295064 74.051 158 41 0 1 158 68 225 2.60e-81 239
Msa1218200 MtrunA17_Chr2g0295021 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa1218200 MtrunA17_Chr2g0295001 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa1218200 MtrunA17_Chr2g0295051 70.064 157 47 0 1 157 1 157 7.62e-78 228
Msa1218200 MtrunA17_Chr2g0295041 68.354 158 50 0 1 158 1 158 1.47e-75 222
Msa1218200 MtrunA17_Chr2g0295141 63.694 157 57 0 1 157 1 157 1.35e-68 204
Msa1218200 MtrunA17_Chr2g0295104 64.331 157 56 0 1 157 1 157 2.52e-68 204
Msa1218200 MtrunA17_Chr2g0295091 64.331 157 56 0 1 157 1 157 8.50e-68 202
Msa1218200 MtrunA17_Chr2g0295124 64.968 157 55 0 1 157 1 157 8.66e-67 200
Msa1218200 MtrunA17_Chr2g0295081 54.140 157 72 0 1 157 1 157 1.81e-54 169
Msa1218200 MtrunA17_Chr4g0067921 45.963 161 82 4 1 158 1 159 2.51e-37 125
Msa1218200 MtrunA17_Chr2g0295211 37.179 156 97 1 1 155 1 156 2.68e-33 115
Msa1218200 MtrunA17_Chr4g0067841 36.943 157 98 1 1 156 1 157 1.02e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACATAAATATTATT+TGG 0.112159 8_1:+80750098 None:intergenic
CCATGTTTGGTTTGATAATT+TGG 0.145886 8_1:+80750970 None:intergenic
AAAACAACATAAATCTTATT+TGG 0.160723 8_1:+80750128 None:intergenic
TTTGGTGATGAAGTTGTAAA+AGG 0.190525 8_1:+80750988 None:intergenic
CTCCATCAATTGCATCAATT+TGG 0.202998 8_1:+80750601 None:intergenic
TTGATGGCATCAACAGCTTT+TGG 0.227157 8_1:+80750858 None:intergenic
GATATCATTTGAAGCGAAAT+TGG 0.228714 8_1:-80750517 Msa1218200:CDS
TAGATGGATTAGTTGTAATT+AGG 0.259719 8_1:+80750345 None:intergenic
GGCCATTGAGGGTTATGTTT+TGG 0.293726 8_1:-80750373 Msa1218200:CDS
AACGGTTTCAACACTCTTGA+TGG 0.328088 8_1:+80750842 None:intergenic
GGTCCAAATGGAGGGTCTAT+TGG 0.333504 8_1:-80750489 Msa1218200:CDS
TCCTTTGGTTTGATATTTAA+CGG 0.347214 8_1:+80750458 None:intergenic
TGTTGTTAAGTTTATTTACT+TGG 0.354046 8_1:-80750043 Msa1218200:three_prime_UTR
CACCAAATTGATGCAATTGA+TGG 0.359913 8_1:-80750603 Msa1218200:CDS
TAGTAGGTAGACTTGGTAGA+TGG 0.361526 8_1:+80750329 None:intergenic
CTTTGAACAACAACCGGTAA+TGG 0.364496 8_1:+80749989 None:intergenic
GTCCAAATGGAGGGTCTATT+GGG 0.390136 8_1:-80750488 Msa1218200:CDS
AATTGGCTTAGCATCTCCTT+TGG 0.398977 8_1:+80750443 None:intergenic
TATAGTATAGTTGGAGGAGT+AGG 0.400784 8_1:-80750561 Msa1218200:CDS
TGCAATTGATGGAGCAAACT+TGG 0.407405 8_1:-80750592 Msa1218200:CDS
AATCAATAAATTAATACAAA+TGG 0.412812 8_1:-80750160 Msa1218200:three_prime_UTR
TTGAGAGCAAAATCCATTAC+CGG 0.415935 8_1:-80750002 Msa1218200:three_prime_UTR
TTCCCAATAGACCCTCCATT+TGG 0.416216 8_1:+80750486 None:intergenic
AATGTGAATACACCCATGTT+TGG 0.426195 8_1:+80750957 None:intergenic
AGAGCTTTGTAAAGCTTAGC+AGG 0.435032 8_1:+80750906 None:intergenic
AACGAAAGTGAGCTTCTTGA+CGG 0.435771 8_1:+80750797 None:intergenic
ACAGATGATAGTAGATTAGT+AGG 0.436784 8_1:+80750313 None:intergenic
TTGGTGATGAAGTTGTAAAA+GGG 0.438115 8_1:+80750989 None:intergenic
AAGAAGCTCACTTTCGTTGA+GGG 0.454166 8_1:-80750792 Msa1218200:intron
GGATTAGTTGTAATTAGGAT+TGG 0.464473 8_1:+80750350 None:intergenic
GTGAGCTTCTTGACGGTGCC+AGG 0.467117 8_1:+80750804 None:intergenic
CCAAATTATCAAACCAAACA+TGG 0.473191 8_1:-80750970 Msa1218200:exon
GGTTGAGGAAGGCAAGGCAA+AGG 0.481278 8_1:-80750412 Msa1218200:CDS
GTTGAAACCGTTGAGGGAAA+TGG 0.483004 8_1:-80750831 Msa1218200:CDS
TATGTATGTTGGTGTGTGGC+TGG 0.486530 8_1:+80751050 None:intergenic
TTTGAAGCGAAATTGGTTGA+AGG 0.487809 8_1:-80750510 Msa1218200:CDS
TTGTTCAAAGCAAAATATCA+AGG 0.488358 8_1:-80749976 Msa1218200:three_prime_UTR
GGGTATAACTATAGTATAGT+TGG 0.489142 8_1:-80750570 Msa1218200:CDS
ATAGTATAGTTGGAGGAGTA+GGG 0.491450 8_1:-80750560 Msa1218200:CDS
AGTAGATTAGTAGGTAGACT+TGG 0.500749 8_1:+80750322 None:intergenic
AGATGCTAAGCCAATTGAAA+AGG 0.515464 8_1:-80750436 Msa1218200:CDS
GTTGAGGGAAATGGTGGTCC+TGG 0.519714 8_1:-80750822 Msa1218200:CDS
GCCAATTGAAAAGGAGGTTG+AGG 0.520188 8_1:-80750427 Msa1218200:CDS
GGCCAAAACATAACCCTCAA+TGG 0.521409 8_1:+80750371 None:intergenic
CAAGAAGCTCACTTTCGTTG+AGG 0.534631 8_1:-80750793 Msa1218200:CDS
GCAATTGATGGAGCAAACTT+GGG 0.541306 8_1:-80750591 Msa1218200:CDS
GTTGAGGAAGGCAAGGCAAA+GGG 0.544771 8_1:-80750411 Msa1218200:CDS
AAGAGTGTTGAAACCGTTGA+GGG 0.546792 8_1:-80750837 Msa1218200:CDS
AATTTGGTGCAACACGTACA+AGG 0.554848 8_1:+80750617 None:intergenic
TGAGAACAAGATATGTATGT+TGG 0.557908 8_1:+80751039 None:intergenic
CAACAAAACACCAAATCTCC+TGG 0.558696 8_1:+80750197 None:intergenic
GGATGATATCATCAGAGTCA+TGG 0.567722 8_1:+80750880 None:intergenic
AAATTGGTTGAAGGTCCAAA+TGG 0.568255 8_1:-80750501 Msa1218200:CDS
AGGACCACCATTTCCCTCAA+CGG 0.572788 8_1:+80750824 None:intergenic
TATAACTATAGTATAGTTGG+AGG 0.584231 8_1:-80750567 Msa1218200:CDS
AGCAGGAGCAACTGTAGAGG+TGG 0.585421 8_1:+80750923 None:intergenic
CTTAGCAGGAGCAACTGTAG+AGG 0.591469 8_1:+80750920 None:intergenic
TGCTAAGCCAATTGAAAAGG+AGG 0.591628 8_1:-80750433 Msa1218200:CDS
ACCGTTAAATATCAAACCAA+AGG 0.596206 8_1:-80750459 Msa1218200:CDS
AGGAGTAGGGTTGCCAGACA+CGG 0.598102 8_1:-80750547 Msa1218200:CDS
TGGTTGAAGGTCCAAATGGA+GGG 0.601542 8_1:-80750497 Msa1218200:CDS
TTGGTTGAAGGTCCAAATGG+AGG 0.609517 8_1:-80750498 Msa1218200:CDS
ATTGAAAAGGAGGTTGAGGA+AGG 0.616285 8_1:-80750423 Msa1218200:CDS
CAAGAGTGTTGAAACCGTTG+AGG 0.622991 8_1:-80750838 Msa1218200:CDS
AAAGGAGGTTGAGGAAGGCA+AGG 0.629508 8_1:-80750418 Msa1218200:CDS
CAATTGATGGAGCAAACTTG+GGG 0.644212 8_1:-80750590 Msa1218200:CDS
CAAATTATCAAACCAAACAT+GGG 0.651382 8_1:-80750969 Msa1218200:exon
AAGATATGTATGTTGGTGTG+TGG 0.694182 8_1:+80751046 None:intergenic
GAAACCGTTGAGGGAAATGG+TGG 0.710996 8_1:-80750828 Msa1218200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATAAATTAATACAAA+TGG - chr8_1:80750865-80750884 Msa1218200:CDS 10.0%
!! AATCAATAAATTAATACAAA+TGG - chr8_1:80750865-80750884 Msa1218200:CDS 10.0%
!! AAGAAAAAACTTTATTACAT+TGG + chr8_1:80750783-80750802 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG - chr8_1:80750787-80750806 Msa1218200:intron 15.0%
!! AAAACAACATAAATCTTATT+TGG + chr8_1:80750900-80750919 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG + chr8_1:80750930-80750949 None:intergenic 15.0%
!! AAGAAAAAACTTTATTACAT+TGG + chr8_1:80750783-80750802 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG - chr8_1:80750787-80750806 Msa1218200:intron 15.0%
!! AAAACAACATAAATCTTATT+TGG + chr8_1:80750900-80750919 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG + chr8_1:80750930-80750949 None:intergenic 15.0%
!! TGTTGTTAAGTTTATTTACT+TGG - chr8_1:80750982-80751001 Msa1218200:five_prime_UTR 20.0%
!! TGTTGTTAAGTTTATTTACT+TGG - chr8_1:80750982-80751001 Msa1218200:five_prime_UTR 20.0%
! CAAATTATCAAACCAAACAT+GGG - chr8_1:80750056-80750075 Msa1218200:three_prime_UTR 25.0%
! TATAACTATAGTATAGTTGG+AGG - chr8_1:80750458-80750477 Msa1218200:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG + chr8_1:80750570-80750589 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG + chr8_1:80750683-80750702 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG - chr8_1:80751049-80751068 Msa1218200:five_prime_UTR 25.0%
! CAAATTATCAAACCAAACAT+GGG - chr8_1:80750056-80750075 Msa1218200:three_prime_UTR 25.0%
! TATAACTATAGTATAGTTGG+AGG - chr8_1:80750458-80750477 Msa1218200:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG + chr8_1:80750570-80750589 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG + chr8_1:80750683-80750702 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG - chr8_1:80751049-80751068 Msa1218200:five_prime_UTR 25.0%
TGAGAACAAGATATGTATGT+TGG + chr8_1:80749989-80750008 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG + chr8_1:80750039-80750058 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG + chr8_1:80750040-80750059 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG + chr8_1:80750058-80750077 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG - chr8_1:80750055-80750074 Msa1218200:three_prime_UTR 30.0%
!! TGTCGCACATGTTTTTAATA+TGG - chr8_1:80750315-80750334 Msa1218200:three_prime_UTR 30.0%
GGGTATAACTATAGTATAGT+TGG - chr8_1:80750455-80750474 Msa1218200:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG - chr8_1:80750508-80750527 Msa1218200:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG - chr8_1:80750566-80750585 Msa1218200:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG + chr8_1:80750678-80750697 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG + chr8_1:80750715-80750734 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG - chr8_1:80750810-80750829 Msa1218200:CDS 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG + chr8_1:80751045-80751064 None:intergenic 30.0%
TGAGAACAAGATATGTATGT+TGG + chr8_1:80749989-80750008 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG + chr8_1:80750039-80750058 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG + chr8_1:80750040-80750059 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG + chr8_1:80750058-80750077 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG - chr8_1:80750055-80750074 Msa1218200:three_prime_UTR 30.0%
!! TGTCGCACATGTTTTTAATA+TGG - chr8_1:80750315-80750334 Msa1218200:three_prime_UTR 30.0%
GGGTATAACTATAGTATAGT+TGG - chr8_1:80750455-80750474 Msa1218200:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG - chr8_1:80750508-80750527 Msa1218200:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG - chr8_1:80750566-80750585 Msa1218200:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG + chr8_1:80750678-80750697 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG + chr8_1:80750715-80750734 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG - chr8_1:80750810-80750829 Msa1218200:CDS 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG + chr8_1:80751045-80751064 None:intergenic 30.0%
AAGATATGTATGTTGGTGTG+TGG + chr8_1:80749982-80750001 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG + chr8_1:80750071-80750090 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG - chr8_1:80750377-80750396 Msa1218200:CDS 35.0%
TCCTGCGTACATATTTATGT+TGG + chr8_1:80750381-80750400 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG - chr8_1:80750380-80750399 Msa1218200:CDS 35.0%
ACATAAATATGTACGCAGGA+GGG - chr8_1:80750381-80750400 Msa1218200:CDS 35.0%
CACCAAATTGATGCAATTGA+TGG - chr8_1:80750422-80750441 Msa1218200:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG + chr8_1:80750427-80750446 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG - chr8_1:80750464-80750483 Msa1218200:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG - chr8_1:80750465-80750484 Msa1218200:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG - chr8_1:80750515-80750534 Msa1218200:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG - chr8_1:80750524-80750543 Msa1218200:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG - chr8_1:80750589-80750608 Msa1218200:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG + chr8_1:80750706-80750725 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG - chr8_1:80751023-80751042 Msa1218200:five_prime_UTR 35.0%
AAGATATGTATGTTGGTGTG+TGG + chr8_1:80749982-80750001 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG + chr8_1:80750071-80750090 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG - chr8_1:80750377-80750396 Msa1218200:CDS 35.0%
TCCTGCGTACATATTTATGT+TGG + chr8_1:80750381-80750400 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG - chr8_1:80750380-80750399 Msa1218200:CDS 35.0%
ACATAAATATGTACGCAGGA+GGG - chr8_1:80750381-80750400 Msa1218200:CDS 35.0%
CACCAAATTGATGCAATTGA+TGG - chr8_1:80750422-80750441 Msa1218200:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG + chr8_1:80750427-80750446 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG - chr8_1:80750464-80750483 Msa1218200:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG - chr8_1:80750465-80750484 Msa1218200:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG - chr8_1:80750515-80750534 Msa1218200:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG - chr8_1:80750524-80750543 Msa1218200:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG - chr8_1:80750589-80750608 Msa1218200:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG + chr8_1:80750706-80750725 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG - chr8_1:80751023-80751042 Msa1218200:five_prime_UTR 35.0%
! AGAGCTTTGTAAAGCTTAGC+AGG + chr8_1:80750122-80750141 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG + chr8_1:80750148-80750167 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG + chr8_1:80750169-80750188 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG + chr8_1:80750170-80750189 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG + chr8_1:80750186-80750205 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG - chr8_1:80750188-80750207 Msa1218200:three_prime_UTR 40.0%
AACGAAAGTGAGCTTCTTGA+CGG + chr8_1:80750231-80750250 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG - chr8_1:80750233-80750252 Msa1218200:three_prime_UTR 40.0%
!! AATTTGGTGCAACACGTACA+AGG + chr8_1:80750411-80750430 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG - chr8_1:80750433-80750452 Msa1218200:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG - chr8_1:80750434-80750453 Msa1218200:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG - chr8_1:80750435-80750454 Msa1218200:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG + chr8_1:80750494-80750513 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG + chr8_1:80750585-80750604 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG - chr8_1:80750592-80750611 Msa1218200:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG + chr8_1:80750602-80750621 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG - chr8_1:80750602-80750621 Msa1218200:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG - chr8_1:80750631-80750650 Msa1218200:intron 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG + chr8_1:80750699-80750718 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG - chr8_1:80750818-80750837 Msa1218200:CDS 40.0%
CAACAAAACACCAAATCTCC+TGG + chr8_1:80750831-80750850 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG + chr8_1:80751039-80751058 None:intergenic 40.0%
! AGAGCTTTGTAAAGCTTAGC+AGG + chr8_1:80750122-80750141 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG + chr8_1:80750148-80750167 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG + chr8_1:80750169-80750188 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG + chr8_1:80750170-80750189 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG + chr8_1:80750186-80750205 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG - chr8_1:80750188-80750207 Msa1218200:three_prime_UTR 40.0%
AACGAAAGTGAGCTTCTTGA+CGG + chr8_1:80750231-80750250 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG - chr8_1:80750233-80750252 Msa1218200:three_prime_UTR 40.0%
!! AATTTGGTGCAACACGTACA+AGG + chr8_1:80750411-80750430 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG - chr8_1:80750433-80750452 Msa1218200:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG - chr8_1:80750434-80750453 Msa1218200:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG - chr8_1:80750435-80750454 Msa1218200:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG + chr8_1:80750494-80750513 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG + chr8_1:80750585-80750604 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG - chr8_1:80750592-80750611 Msa1218200:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG + chr8_1:80750602-80750621 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG - chr8_1:80750602-80750621 Msa1218200:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG - chr8_1:80750631-80750650 Msa1218200:intron 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG + chr8_1:80750699-80750718 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG - chr8_1:80750818-80750837 Msa1218200:CDS 40.0%
CAACAAAACACCAAATCTCC+TGG + chr8_1:80750831-80750850 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG + chr8_1:80751039-80751058 None:intergenic 40.0%
TATGTATGTTGGTGTGTGGC+TGG + chr8_1:80749978-80749997 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG - chr8_1:80750187-80750206 Msa1218200:three_prime_UTR 45.0%
GTTGAAACCGTTGAGGGAAA+TGG - chr8_1:80750194-80750213 Msa1218200:three_prime_UTR 45.0%
CAAGAAGCTCACTTTCGTTG+AGG - chr8_1:80750232-80750251 Msa1218200:three_prime_UTR 45.0%
TTGGTTGAAGGTCCAAATGG+AGG - chr8_1:80750527-80750546 Msa1218200:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG - chr8_1:80750528-80750547 Msa1218200:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG - chr8_1:80750537-80750556 Msa1218200:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG + chr8_1:80750542-80750561 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG - chr8_1:80750598-80750617 Msa1218200:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG - chr8_1:80750640-80750659 Msa1218200:intron 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG - chr8_1:80750641-80750660 Msa1218200:intron 45.0%
! GGCCATTGAGGGTTATGTTT+TGG - chr8_1:80750652-80750671 Msa1218200:intron 45.0%
GGCCAAAACATAACCCTCAA+TGG + chr8_1:80750657-80750676 None:intergenic 45.0%
TATGTATGTTGGTGTGTGGC+TGG + chr8_1:80749978-80749997 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG - chr8_1:80750187-80750206 Msa1218200:three_prime_UTR 45.0%
GTTGAAACCGTTGAGGGAAA+TGG - chr8_1:80750194-80750213 Msa1218200:three_prime_UTR 45.0%
CAAGAAGCTCACTTTCGTTG+AGG - chr8_1:80750232-80750251 Msa1218200:three_prime_UTR 45.0%
TTGGTTGAAGGTCCAAATGG+AGG - chr8_1:80750527-80750546 Msa1218200:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG - chr8_1:80750528-80750547 Msa1218200:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG - chr8_1:80750537-80750556 Msa1218200:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG + chr8_1:80750542-80750561 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG - chr8_1:80750598-80750617 Msa1218200:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG - chr8_1:80750640-80750659 Msa1218200:intron 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG - chr8_1:80750641-80750660 Msa1218200:intron 45.0%
! GGCCATTGAGGGTTATGTTT+TGG - chr8_1:80750652-80750671 Msa1218200:intron 45.0%
GGCCAAAACATAACCCTCAA+TGG + chr8_1:80750657-80750676 None:intergenic 45.0%
CTTAGCAGGAGCAACTGTAG+AGG + chr8_1:80750108-80750127 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG - chr8_1:80750197-80750216 Msa1218200:three_prime_UTR 50.0%
AGGACCACCATTTCCCTCAA+CGG + chr8_1:80750204-80750223 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG - chr8_1:80750536-80750555 Msa1218200:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG - chr8_1:80750607-80750626 Msa1218200:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG - chr8_1:80750614-80750633 Msa1218200:CDS 50.0%
CTTAGCAGGAGCAACTGTAG+AGG + chr8_1:80750108-80750127 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG - chr8_1:80750197-80750216 Msa1218200:three_prime_UTR 50.0%
AGGACCACCATTTCCCTCAA+CGG + chr8_1:80750204-80750223 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG - chr8_1:80750536-80750555 Msa1218200:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG - chr8_1:80750607-80750626 Msa1218200:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG - chr8_1:80750614-80750633 Msa1218200:CDS 50.0%
AGCAGGAGCAACTGTAGAGG+TGG + chr8_1:80750105-80750124 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG - chr8_1:80750203-80750222 Msa1218200:three_prime_UTR 55.0%
AGGAGTAGGGTTGCCAGACA+CGG - chr8_1:80750478-80750497 Msa1218200:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG - chr8_1:80750613-80750632 Msa1218200:CDS 55.0%
AGCAGGAGCAACTGTAGAGG+TGG + chr8_1:80750105-80750124 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG - chr8_1:80750203-80750222 Msa1218200:three_prime_UTR 55.0%
AGGAGTAGGGTTGCCAGACA+CGG - chr8_1:80750478-80750497 Msa1218200:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG - chr8_1:80750613-80750632 Msa1218200:CDS 55.0%
GTGAGCTTCTTGACGGTGCC+AGG + chr8_1:80750224-80750243 None:intergenic 60.0%
GTGAGCTTCTTGACGGTGCC+AGG + chr8_1:80750224-80750243 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 80749972 80751075 80749972 ID=Msa1218200;Name=Msa1218200
chr8_1 mRNA 80749972 80751075 80749972 ID=Msa1218200-mRNA-1;Parent=Msa1218200;Name=Msa1218200-mRNA-1;_AED=0.07;_eAED=0.07;_QI=102|1|1|1|1|1|2|381|158
chr8_1 exon 80749972 80750648 80749972 ID=Msa1218200-mRNA-1:exon:28391;Parent=Msa1218200-mRNA-1
chr8_1 exon 80750793 80751075 80750793 ID=Msa1218200-mRNA-1:exon:28390;Parent=Msa1218200-mRNA-1
chr8_1 five_prime_UTR 80750974 80751075 80750974 ID=Msa1218200-mRNA-1:five_prime_utr;Parent=Msa1218200-mRNA-1
chr8_1 CDS 80750793 80750973 80750793 ID=Msa1218200-mRNA-1:cds;Parent=Msa1218200-mRNA-1
chr8_1 CDS 80750353 80750648 80750353 ID=Msa1218200-mRNA-1:cds;Parent=Msa1218200-mRNA-1
chr8_1 three_prime_UTR 80749972 80750352 80749972 ID=Msa1218200-mRNA-1:three_prime_utr;Parent=Msa1218200-mRNA-1
Gene Sequence

>Msa1218200

ATGGGTGTATTCACATTTGAGCAAGAAACCACCTCTACAGTTGCTCCTGCTAAGCTTTACAAAGCTCTTGTCCATGACTCTGATGATATCATCCCAAAAGCTGTTGATGCCATCAAGAGTGTTGAAACCGTTGAGGGAAATGGTGGTCCTGGCACCGTCAAGAAGCTCACTTTCGTTGAGGGAGGGCAAACCTTGTACGTGTTGCACCAAATTGATGCAATTGATGGAGCAAACTTGGGGTATAACTATAGTATAGTTGGAGGAGTAGGGTTGCCAGACACGGTTGAAAAGATATCATTTGAAGCGAAATTGGTTGAAGGTCCAAATGGAGGGTCTATTGGGAAGACGACCGTTAAATATCAAACCAAAGGAGATGCTAAGCCAATTGAAAAGGAGGTTGAGGAAGGCAAGGCAAAGGGTGATGCTCTTTTCAAGGCCATTGAGGGTTATGTTTTGGCCAATCCTAATTACAACTAA
Protein sequence

>Msa1218200

MGVFTFEQETTSTVAPAKLYKALVHDSDDIIPKAVDAIKSVETVEGNGGPGTVKKLTFVEGGQTLYVLHQIDAIDGANLGYNYSIVGGVGLPDTVEKISFEAKLVEGPNGGSIGKTTVKYQTKGDAKPIEKEVEEGKAKGDALFKAIEGYVLANPNYN*