AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0929090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929090 MtrunA17_Chr6g0473621 95.810 358 10 4 1 356 1 355 0.0 691
Msa0929090 MtrunA17_Chr1g0203771 63.158 361 100 7 1 356 1 333 8.01e-143 407
Msa0929090 MtrunA17_Chr4g0057261 47.541 305 110 5 23 321 20 280 1.61e-79 244
Msa0929090 MtrunA17_Chr2g0285591 46.053 228 118 2 10 237 7 229 7.24e-65 206
Msa0929090 MtrunA17_Chr7g0243051 45.000 220 118 2 19 237 3 220 1.88e-55 181
Msa0929090 MtrunA17_Chr4g0007601 49.462 186 91 1 60 242 27 212 2.18e-51 171
Msa0929090 MtrunA17_Chr6g0458271 41.727 139 75 4 18 156 23 155 6.21e-27 104
Msa0929090 MtrunA17_Chr6g0458281 39.516 124 70 1 34 157 35 153 7.24e-25 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929090 AT5G52060.1 54.192 334 111 7 35 356 39 342 3.48e-113 333
Msa0929090 AT5G07220.1 51.312 343 118 8 17 356 7 303 3.12e-104 309
Msa0929090 AT5G62100.2 48.182 330 108 6 32 355 12 284 1.99e-96 288
Msa0929090 AT5G62100.1 46.921 341 107 8 32 355 12 295 6.64e-93 280
Msa0929090 AT5G62100.5 63.830 188 65 1 32 219 12 196 1.84e-81 247
Msa0929090 AT5G62100.4 62.963 189 67 1 31 219 11 196 7.22e-81 246
Msa0929090 AT5G62100.3 64.286 140 47 1 32 171 12 148 3.17e-59 188
Msa0929090 AT5G62100.6 64.286 140 47 1 32 171 12 148 3.17e-59 188
Msa0929090 AT3G51780.1 43.119 218 105 5 35 237 10 223 2.07e-45 157
Msa0929090 AT5G14360.1 41.818 110 59 1 34 143 47 151 7.90e-22 91.3
Msa0929090 AT5G40630.1 36.937 111 67 2 33 143 54 161 1.73e-20 87.8

Find 121 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGAAGTTCTTGAGAAAA+TGG 0.175572 6_2:+76299300 None:intergenic
TAGTCAAGAGAAAAGGATTT+TGG 0.200313 6_2:-76297872 Msa0929090:CDS
GATGAATCAATTGCTTAAAT+TGG 0.225045 6_2:-76297263 Msa0929090:CDS
TCATGTAAATGACATATTAT+AGG 0.232891 6_2:+76296311 None:intergenic
CATGTAAATGACATATTATA+GGG 0.256093 6_2:+76296312 None:intergenic
GAGTTGTTGTTACTACAAAA+TGG 0.278243 6_2:-76296566 Msa0929090:CDS
CTGCAACCACCTGAGCTTAT+AGG 0.279994 6_2:+76298620 None:intergenic
CTTAGGAGGACCTGAATTAT+TGG 0.280440 6_2:+76296470 None:intergenic
TGGTTCCTACAATTAGAGTT+AGG 0.283648 6_2:-76298508 Msa0929090:CDS
CTGTAAAGATCTTTGCTTTG+AGG 0.303482 6_2:-76299114 Msa0929090:five_prime_UTR
GAATTCATTTACCAAATGTT+TGG 0.308274 6_2:-76296215 Msa0929090:three_prime_UTR
ATCCCATTCTTGATCGATTT+CGG 0.309671 6_2:+76296118 None:intergenic
TTGATCGATTTCGGTTGGTT+GGG 0.331578 6_2:+76296127 None:intergenic
AGGTGGTTGCAGAGCTGAAC+CGG 0.333790 6_2:-76298609 Msa0929090:CDS
TCTTCTTGTTGTTCTTGAAT+TGG 0.338014 6_2:+76296654 None:intergenic
ATTCATTCAAATGGTTATCA+TGG 0.341230 6_2:-76296687 Msa0929090:CDS
GCTAAGAATTCTATACAAAA+TGG 0.347968 6_2:-76296795 Msa0929090:CDS
AAACATTTGGTAAATGAATT+CGG 0.349014 6_2:+76296217 None:intergenic
CTTGATCGATTTCGGTTGGT+TGG 0.369939 6_2:+76296126 None:intergenic
GAACTTGTGTGGATATGATT+TGG 0.371580 6_2:-76296401 Msa0929090:three_prime_UTR
AATTCATTTACCAAATGTTT+GGG 0.374969 6_2:-76296214 Msa0929090:three_prime_UTR
GAGAATCAATTCCTCATCAT+TGG 0.385614 6_2:-76299278 Msa0929090:five_prime_UTR
AGACAAAGAGAGGGATTCTA+AGG 0.387250 6_2:-76297956 Msa0929090:CDS
CCTTCAATGACCAATAATTC+AGG 0.387889 6_2:-76296480 Msa0929090:CDS
AACATGTTCTAGCAGCCTTT+TGG 0.390705 6_2:-76298899 Msa0929090:five_prime_UTR
GTAGATGGAAATGTGATTAA+TGG 0.398757 6_2:+76296522 None:intergenic
ATTGTGGACTTGTGGAATGC+TGG 0.399723 6_2:-76298938 Msa0929090:five_prime_UTR
ATTAGAGTTAGGGTCAAGTT+TGG 0.404587 6_2:-76298497 Msa0929090:CDS
TTCAAATCGTAACCCTGCTT+TGG 0.405274 6_2:-76298528 Msa0929090:CDS
GAAGAATCAATAACTTTAGT+AGG 0.406720 6_2:+76299052 None:intergenic
ATTAGTTCACAAGCCACATT+TGG 0.410487 6_2:-76298452 Msa0929090:intron
AGATTAGCTTGGAAGTAGAT+AGG 0.410787 6_2:-76297801 Msa0929090:CDS
CCTCAACAAAAGCATTCAAA+TGG 0.422309 6_2:-76296747 Msa0929090:CDS
GGTTCCTACAATTAGAGTTA+GGG 0.435115 6_2:-76298507 Msa0929090:CDS
CTAATTGTAGGAACCAAAGC+AGG 0.435383 6_2:+76298515 None:intergenic
TTGACCCTAACTCTAATTGT+AGG 0.437016 6_2:+76298503 None:intergenic
TTGGAAGTAGATAGGCTTGC+CGG 0.437951 6_2:-76297793 Msa0929090:CDS
AAAATGTTGTCAGGACCAAC+TGG 0.440623 6_2:-76298012 Msa0929090:CDS
CCACAACAAATTCATTCAAA+TGG 0.444236 6_2:-76296696 Msa0929090:CDS
CCTGAATTATTGGTCATTGA+AGG 0.451892 6_2:+76296480 None:intergenic
CATGGTAATAATAAGACAAA+TGG 0.457366 6_2:-76298653 Msa0929090:CDS
CAGAGCTGAACCGGGTTCAA+TGG 0.458677 6_2:-76298600 Msa0929090:CDS
CAACCGAAATCGATCAAGAA+TGG 0.459535 6_2:-76296121 Msa0929090:three_prime_UTR
AAGAATTCCCAACTGAACTT+AGG 0.466020 6_2:+76296453 None:intergenic
AGTAGATAGGCTTGCCGGAC+GGG 0.466043 6_2:-76297788 Msa0929090:intron
AGTTGTTGTTACTACAAAAT+GGG 0.466295 6_2:-76296565 Msa0929090:CDS
TTGTGGACTTGTGGAATGCT+GGG 0.467379 6_2:-76298937 Msa0929090:five_prime_UTR
TGATCGATTTCGGTTGGTTG+GGG 0.469402 6_2:+76296128 None:intergenic
AGAATGGGATGGTGAGTTGT+TGG 0.472145 6_2:-76296105 None:intergenic
GAAAGAAGATGTTGAGGATG+AGG 0.474398 6_2:-76298688 Msa0929090:exon
TTGTGGAATGCTGGGGTCAG+AGG 0.476312 6_2:-76298929 Msa0929090:five_prime_UTR
TCTTGATGTAGTTGGTGTAA+AGG 0.476551 6_2:-76297929 Msa0929090:CDS
CATTCTTGATCGATTTCGGT+TGG 0.477126 6_2:+76296122 None:intergenic
CTGAACCGGGTTCAATGGAA+TGG 0.478419 6_2:-76298595 Msa0929090:CDS
AATCAATTGCTTAAATTGGA+TGG 0.481120 6_2:-76297259 Msa0929090:CDS
CTCTCGTAATTGTGGACTTG+TGG 0.486669 6_2:-76298946 Msa0929090:five_prime_UTR
AATGTGGCTGAAAATATTGA+TGG 0.487707 6_2:+76299140 None:intergenic
GAAGTTCTTGAGAAAATGGA+AGG 0.488465 6_2:+76299304 None:intergenic
ATCCTATTAGTCAAGAGAAA+AGG 0.488781 6_2:-76297879 Msa0929090:CDS
ATCTATCTCACAGATTAGCT+TGG 0.490471 6_2:-76297812 Msa0929090:CDS
TCAAAAGTATGTTGAAACAT+TGG 0.493311 6_2:-76296829 Msa0929090:CDS
GTTTCTTCACCTATAAGCTC+AGG 0.498812 6_2:-76298629 Msa0929090:CDS
CACCGACCCAATTCACCAAA+AGG 0.500699 6_2:+76298884 None:intergenic
CAGGTCCTCCTAAGTTCAGT+TGG 0.500744 6_2:-76296461 Msa0929090:CDS
GATTTGAAGAAAATGTTGTC+AGG 0.501668 6_2:-76298021 Msa0929090:CDS
ATTGAGGAAGAATGCTAAGA+TGG 0.502442 6_2:-76297848 Msa0929090:CDS
AACCGAAATCGATCAAGAAT+GGG 0.508928 6_2:-76296120 Msa0929090:three_prime_UTR
TTCATTCAAATGGTTATCAT+GGG 0.511689 6_2:-76296686 Msa0929090:CDS
ATTTGGATTTGTATTCCACT+TGG 0.516673 6_2:-76296384 Msa0929090:three_prime_UTR
ATGGAATGGGAGATGAGGCC+AGG 0.517312 6_2:-76298581 Msa0929090:CDS
GTGAAAAGATTTACCAAATG+TGG 0.519912 6_2:+76298439 None:intergenic
GAATCAATAATTAGCAAAGG+TGG 0.522143 6_2:-76297328 Msa0929090:CDS
AATGAGTGGCCAGCTAACTT+AGG 0.522849 6_2:+76299378 None:intergenic
ATGAGGAATAATCATAATCA+TGG 0.527299 6_2:-76298671 Msa0929090:CDS
CTCAACAAAAGCATTCAAAT+GGG 0.530855 6_2:-76296746 Msa0929090:CDS
GCATTCAAATGGGAAACGAT+TGG 0.532681 6_2:-76296736 Msa0929090:CDS
AGGTCCTCCTAAGTTCAGTT+GGG 0.534106 6_2:-76296460 Msa0929090:CDS
GATGAAATCAATGAGAAGCT+AGG 0.534284 6_2:+76299235 None:intergenic
AATCAATAATTAGCAAAGGT+GGG 0.534823 6_2:-76297327 Msa0929090:CDS
GATGGAAATGTGATTAATGG+TGG 0.538741 6_2:+76296525 None:intergenic
TGAACCGGGTTCAATGGAAT+GGG 0.545913 6_2:-76298594 Msa0929090:CDS
CAAATCTAAACTAGTTCTTG+TGG 0.548510 6_2:-76297905 Msa0929090:CDS
TTTGAATCAATAATTAGCAA+AGG 0.549282 6_2:-76297331 Msa0929090:CDS
TCACAACTATGCAACAAAAG+TGG 0.555123 6_2:+76298715 None:intergenic
TCTTCACCTATAAGCTCAGG+TGG 0.558010 6_2:-76298626 Msa0929090:CDS
AGGGTGTCACCTAAGTTAGC+TGG 0.564041 6_2:-76299387 None:intergenic
GAACAACAACACCAACCCTC+AGG 0.567003 6_2:-76296588 Msa0929090:CDS
ATCTCCCATTCCATTGAACC+CGG 0.567397 6_2:+76298590 None:intergenic
AATTCCCAACTGAACTTAGG+AGG 0.568401 6_2:+76296456 None:intergenic
ATGGAAATGTGATTAATGGT+GGG 0.575408 6_2:+76296526 None:intergenic
GTTGTAGAATCAGATGTGCT+TGG 0.578246 6_2:-76297301 Msa0929090:CDS
AAGTTCTTGAGAAAATGGAA+GGG 0.579873 6_2:+76299305 None:intergenic
GATGAGGCCAGGTGGAATGT+TGG 0.583094 6_2:-76298570 Msa0929090:CDS
GTTTGAACCAACATTCCACC+TGG 0.585611 6_2:+76298563 None:intergenic
AGTAAACAATTCCAATGATG+AGG 0.587569 6_2:+76299267 None:intergenic
AAAACATATAACACTCCAAG+TGG 0.590061 6_2:+76296369 None:intergenic
TTGGATGGTATAATTACTGA+TGG 0.591576 6_2:-76297244 Msa0929090:CDS
GTTGTGGATGTAGATGTAGA+TGG 0.595278 6_2:+76296507 None:intergenic
ATGTAAATGACATATTATAG+GGG 0.596777 6_2:+76296313 None:intergenic
CCATTTGAATGAATTTGTTG+TGG 0.596817 6_2:+76296696 None:intergenic
GTAGTAACAACAACTCCTGA+GGG 0.597798 6_2:+76296573 None:intergenic
GCCACATGTTTGAACTTGTG+TGG 0.602693 6_2:-76296412 Msa0929090:three_prime_UTR
GGTGGTTGCAGAGCTGAACC+GGG 0.606693 6_2:-76298608 Msa0929090:CDS
TGTAGTAACAACAACTCCTG+AGG 0.608014 6_2:+76296572 None:intergenic
AGGGTTACGATTTGAATCAG+TGG 0.610632 6_2:+76298535 None:intergenic
AAGTAGATAGGCTTGCCGGA+CGG 0.614233 6_2:-76297789 Msa0929090:CDS
GAGAGAGGGTTAGATCTCTG+AGG 0.620671 6_2:+76298828 None:intergenic
TCCACACAAGTTCAAACATG+TGG 0.629908 6_2:+76296411 None:intergenic
GTTCAATGGAATGGGAGATG+AGG 0.632010 6_2:-76298586 Msa0929090:CDS
GGTCATTGAAGGAGTAGTTG+TGG 0.633027 6_2:+76296491 None:intergenic
AAGATCTTTACAGCAAAATG+TGG 0.634708 6_2:+76299124 None:intergenic
TAACAACAACTCCTGAGGGT+TGG 0.635228 6_2:+76296577 None:intergenic
ACAAGTTCAAACATGTGGCA+TGG 0.644336 6_2:+76296416 None:intergenic
GAAAGTGAAAGAAGATGTTG+AGG 0.648534 6_2:-76298694 Msa0929090:exon
TCTTGATGATGTAAACCAGT+TGG 0.653777 6_2:+76297997 None:intergenic
GAAATCGATCAAGAATGGGA+TGG 0.659934 6_2:-76296116 Msa0929090:three_prime_UTR
GAATGGGAGATGAGGCCAGG+TGG 0.662952 6_2:-76298578 Msa0929090:CDS
TGGAAATGTGATTAATGGTG+GGG 0.663979 6_2:+76296527 None:intergenic
ATGTGGCTGAAAATATTGAT+GGG 0.664034 6_2:+76299141 None:intergenic
TAATTGTAGGAACCAAAGCA+GGG 0.677158 6_2:+76298516 None:intergenic
TGTGGACTTGTGGAATGCTG+GGG 0.714561 6_2:-76298936 Msa0929090:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTTTCTTACTTAT+TGG - chr6_2:76296288-76296307 Msa0929090:three_prime_UTR 10.0%
!! ATTTGATGAATAAATTAAAT+TGG - chr6_2:76297979-76297998 Msa0929090:CDS 10.0%
!! AAAATATCAAAATAAAAACA+AGG + chr6_2:76298317-76298336 None:intergenic 10.0%
!! TTTATATATCTATCAAAAAT+AGG + chr6_2:76299293-76299312 None:intergenic 10.0%
!!! TATTTTTTAAAGTTGTGATT+TGG - chr6_2:76297414-76297433 Msa0929090:intron 15.0%
!!! TATTTTTATATTTTTCCATG+CGG - chr6_2:76298405-76298424 Msa0929090:intron 15.0%
!!! AACAAAATAAACAGTTATTT+TGG + chr6_2:76298555-76298574 None:intergenic 15.0%
!!! TTACTTGTTCATTAATTTTA+TGG - chr6_2:76298592-76298611 Msa0929090:CDS 15.0%
!!! TACTTGTTCATTAATTTTAT+GGG - chr6_2:76298593-76298612 Msa0929090:CDS 15.0%
!! TCATTTACATGAAAAATAAA+TGG - chr6_2:76299181-76299200 Msa0929090:five_prime_UTR 15.0%
!! CATTTACATGAAAAATAAAT+GGG - chr6_2:76299182-76299201 Msa0929090:five_prime_UTR 15.0%
!! ATTTACATGAAAAATAAATG+GGG - chr6_2:76299183-76299202 Msa0929090:five_prime_UTR 15.0%
!!! TTACTTTTATGTGATTATTA+TGG - chr6_2:76299235-76299254 Msa0929090:five_prime_UTR 15.0%
!! TGAATGAATATAAAAATGAG+TGG + chr6_2:76296120-76296139 None:intergenic 20.0%
!!! TTTTTCTTACTTATTGGTTA+AGG - chr6_2:76296294-76296313 Msa0929090:three_prime_UTR 20.0%
!!! TTTGCAGATTAAATTTTTGT+TGG - chr6_2:76296391-76296410 Msa0929090:three_prime_UTR 20.0%
!!! TTGCAGATTAAATTTTTGTT+GGG - chr6_2:76296392-76296411 Msa0929090:three_prime_UTR 20.0%
!! GAAAAAAAACAAAAGATTTG+AGG + chr6_2:76296510-76296529 None:intergenic 20.0%
!! ATCTAATTATTTGTCCATTA+GGG + chr6_2:76297888-76297907 None:intergenic 20.0%
!! TATCTAATTATTTGTCCATT+AGG + chr6_2:76297889-76297908 None:intergenic 20.0%
!!! TAATGGACAAATAATTAGAT+AGG - chr6_2:76297888-76297907 Msa0929090:CDS 20.0%
!! TTTGAATCAATAATTAGCAA+AGG - chr6_2:76298150-76298169 Msa0929090:intron 20.0%
!! ATGTAAATGACATATTATAG+GGG + chr6_2:76299171-76299190 None:intergenic 20.0%
!! CATGTAAATGACATATTATA+GGG + chr6_2:76299172-76299191 None:intergenic 20.0%
!! TCATGTAAATGACATATTAT+AGG + chr6_2:76299173-76299192 None:intergenic 20.0%
!! AAACATTTGGTAAATGAATT+CGG + chr6_2:76299267-76299286 None:intergenic 20.0%
!! AATTCATTTACCAAATGTTT+GGG - chr6_2:76299267-76299286 Msa0929090:five_prime_UTR 20.0%
!! AGATTAAATTTTTGTTGGGT+TGG - chr6_2:76296396-76296415 Msa0929090:three_prime_UTR 25.0%
! GAAGAATCAATAACTTTAGT+AGG + chr6_2:76296432-76296451 None:intergenic 25.0%
! ATGAGGAATAATCATAATCA+TGG - chr6_2:76296810-76296829 Msa0929090:CDS 25.0%
! CATGGTAATAATAAGACAAA+TGG - chr6_2:76296828-76296847 Msa0929090:CDS 25.0%
! TCAAAAGAAGAGAAATTACT+TGG + chr6_2:76297361-76297380 None:intergenic 25.0%
! ACTTTATGAGTCATACAATT+TGG - chr6_2:76297848-76297867 Msa0929090:CDS 25.0%
! TTCAAACAATTATATGAGTG+AGG - chr6_2:76298050-76298069 Msa0929090:intron 25.0%
! ATTGAGTACTAATTAGTTAC+TGG - chr6_2:76298108-76298127 Msa0929090:intron 25.0%
! ATTAGTTACTGGAATTGATT+AGG - chr6_2:76298119-76298138 Msa0929090:intron 25.0%
! AATCAATAATTAGCAAAGGT+GGG - chr6_2:76298154-76298173 Msa0929090:intron 25.0%
! GATGAATCAATTGCTTAAAT+TGG - chr6_2:76298218-76298237 Msa0929090:intron 25.0%
! AATCAATTGCTTAAATTGGA+TGG - chr6_2:76298222-76298241 Msa0929090:intron 25.0%
!!! TCCAATTTGAAACATTTTGA+AGG - chr6_2:76298619-76298638 Msa0929090:CDS 25.0%
! ACCTTCAAAATGTTTCAAAT+TGG + chr6_2:76298623-76298642 None:intergenic 25.0%
!!! TTTGAAACATTTTGAAGGTA+AGG - chr6_2:76298624-76298643 Msa0929090:CDS 25.0%
!! TCAAAAGTATGTTGAAACAT+TGG - chr6_2:76298652-76298671 Msa0929090:CDS 25.0%
! GCTAAGAATTCTATACAAAA+TGG - chr6_2:76298686-76298705 Msa0929090:exon 25.0%
! ATTCATTCAAATGGTTATCA+TGG - chr6_2:76298794-76298813 Msa0929090:five_prime_UTR 25.0%
! TTCATTCAAATGGTTATCAT+GGG - chr6_2:76298795-76298814 Msa0929090:five_prime_UTR 25.0%
!! AGTTGTTGTTACTACAAAAT+GGG - chr6_2:76298916-76298935 Msa0929090:five_prime_UTR 25.0%
! GAATTCATTTACCAAATGTT+TGG - chr6_2:76299266-76299285 Msa0929090:five_prime_UTR 25.0%
AAGTTCTTGAGAAAATGGAA+GGG + chr6_2:76296179-76296198 None:intergenic 30.0%
!! CTTTGAAGTTCTTGAGAAAA+TGG + chr6_2:76296184-76296203 None:intergenic 30.0%
AGTAAACAATTCCAATGATG+AGG + chr6_2:76296217-76296236 None:intergenic 30.0%
ATGTGGCTGAAAATATTGAT+GGG + chr6_2:76296343-76296362 None:intergenic 30.0%
AATGTGGCTGAAAATATTGA+TGG + chr6_2:76296344-76296363 None:intergenic 30.0%
AAGATCTTTACAGCAAAATG+TGG + chr6_2:76296360-76296379 None:intergenic 30.0%
GTGAAAAGATTTACCAAATG+TGG + chr6_2:76297045-76297064 None:intergenic 30.0%
TGTCAAATTATTAGCTGTGT+CGG - chr6_2:76297155-76297174 Msa0929090:intron 30.0%
TCAAAAATCGTAATGAAGCA+TGG + chr6_2:76297204-76297223 None:intergenic 30.0%
TCGAGTTTAAATCTTAACTC+AGG + chr6_2:76297279-76297298 None:intergenic 30.0%
GATTTGAAGAAAATGTTGTC+AGG - chr6_2:76297460-76297479 Msa0929090:intron 30.0%
! TGTTTTACAAAGACAAAGAG+AGG - chr6_2:76297515-76297534 Msa0929090:intron 30.0%
! GTTTTACAAAGACAAAGAGA+GGG - chr6_2:76297516-76297535 Msa0929090:intron 30.0%
! CAAATCTAAACTAGTTCTTG+TGG - chr6_2:76297576-76297595 Msa0929090:intron 30.0%
ATCCTATTAGTCAAGAGAAA+AGG - chr6_2:76297602-76297621 Msa0929090:intron 30.0%
! ATCCTTTTCTCTTGACTAAT+AGG + chr6_2:76297607-76297626 None:intergenic 30.0%
! TAGTCAAGAGAAAAGGATTT+TGG - chr6_2:76297609-76297628 Msa0929090:intron 30.0%
!!! AGAAAAGGATTTTGGAATTG+AGG - chr6_2:76297617-76297636 Msa0929090:intron 30.0%
!!! AATTGGTGATTGACTTGTTT+TGG - chr6_2:76297996-76298015 Msa0929090:CDS 30.0%
AACAATTATATGAGTGAGGT+TGG - chr6_2:76298054-76298073 Msa0929090:intron 30.0%
GAATCAATAATTAGCAAAGG+TGG - chr6_2:76298153-76298172 Msa0929090:intron 30.0%
TTGGATGGTATAATTACTGA+TGG - chr6_2:76298237-76298256 Msa0929090:intron 30.0%
CAAATTGCAAAGAAAAATGC+AGG - chr6_2:76298266-76298285 Msa0929090:intron 30.0%
TCATTGACCAATTTGACTAA+CGG + chr6_2:76298447-76298466 None:intergenic 30.0%
AAATATGAGGTAATACCACT+TGG + chr6_2:76298527-76298546 None:intergenic 30.0%
!!! TATTTTGGTCGCTAAATATG+AGG + chr6_2:76298540-76298559 None:intergenic 30.0%
!! CTCAACAAAAGCATTCAAAT+GGG - chr6_2:76298735-76298754 Msa0929090:five_prime_UTR 30.0%
!! CCATTTGAATGAATTTGTTG+TGG + chr6_2:76298788-76298807 None:intergenic 30.0%
CCACAACAAATTCATTCAAA+TGG - chr6_2:76298785-76298804 Msa0929090:five_prime_UTR 30.0%
!! TCTTCTTGTTGTTCTTGAAT+TGG + chr6_2:76298830-76298849 None:intergenic 30.0%
!!! AGCTCATTTGAGTTTTTTCT+AGG + chr6_2:76298857-76298876 None:intergenic 30.0%
! GAGTTGTTGTTACTACAAAA+TGG - chr6_2:76298915-76298934 Msa0929090:five_prime_UTR 30.0%
ATGGAAATGTGATTAATGGT+GGG + chr6_2:76298958-76298977 None:intergenic 30.0%
GTAGATGGAAATGTGATTAA+TGG + chr6_2:76298962-76298981 None:intergenic 30.0%
ATTTGGATTTGTATTCCACT+TGG - chr6_2:76299097-76299116 Msa0929090:five_prime_UTR 30.0%
AAAACATATAACACTCCAAG+TGG + chr6_2:76299115-76299134 None:intergenic 30.0%
GAAGTTCTTGAGAAAATGGA+AGG + chr6_2:76296180-76296199 None:intergenic 35.0%
GAGAATCAATTCCTCATCAT+TGG - chr6_2:76296203-76296222 Msa0929090:three_prime_UTR 35.0%
GATGAAATCAATGAGAAGCT+AGG + chr6_2:76296249-76296268 None:intergenic 35.0%
CTGTAAAGATCTTTGCTTTG+AGG - chr6_2:76296367-76296386 Msa0929090:three_prime_UTR 35.0%
TCACAACTATGCAACAAAAG+TGG + chr6_2:76296769-76296788 None:intergenic 35.0%
GAAAGTGAAAGAAGATGTTG+AGG - chr6_2:76296787-76296806 Msa0929090:CDS 35.0%
TAATTGTAGGAACCAAAGCA+GGG + chr6_2:76296968-76296987 None:intergenic 35.0%
TGGTTCCTACAATTAGAGTT+AGG - chr6_2:76296973-76296992 Msa0929090:intron 35.0%
GGTTCCTACAATTAGAGTTA+GGG - chr6_2:76296974-76296993 Msa0929090:intron 35.0%
TTGACCCTAACTCTAATTGT+AGG + chr6_2:76296981-76297000 None:intergenic 35.0%
ATTAGAGTTAGGGTCAAGTT+TGG - chr6_2:76296984-76297003 Msa0929090:intron 35.0%
ATTAGTTCACAAGCCACATT+TGG - chr6_2:76297029-76297048 Msa0929090:intron 35.0%
TAATCTTCCGCTATAAAGCA+CGG - chr6_2:76297094-76297113 Msa0929090:intron 35.0%
ATCGTAATGAAGCATGGATA+CGG + chr6_2:76297198-76297217 None:intergenic 35.0%
GACTCATGTAATTCGAAGTT+CGG - chr6_2:76297230-76297249 Msa0929090:CDS 35.0%
!! AAGTTGTGATTTGGTGACAT+TGG - chr6_2:76297423-76297442 Msa0929090:intron 35.0%
!! TCTTGATGATGTAAACCAGT+TGG + chr6_2:76297487-76297506 None:intergenic 35.0%
! AAGGCTTTTCTTGATGTAGT+TGG - chr6_2:76297544-76297563 Msa0929090:intron 35.0%
! TCTTGATGTAGTTGGTGTAA+AGG - chr6_2:76297552-76297571 Msa0929090:intron 35.0%
ATTGAGGAAGAATGCTAAGA+TGG - chr6_2:76297633-76297652 Msa0929090:intron 35.0%
! ATCTATCTCACAGATTAGCT+TGG - chr6_2:76297669-76297688 Msa0929090:intron 35.0%
!! AGATTAGCTTGGAAGTAGAT+AGG - chr6_2:76297680-76297699 Msa0929090:intron 35.0%
TCTATTGCATTACTCCCTAA+TGG - chr6_2:76297871-76297890 Msa0929090:CDS 35.0%
GTGTATTCCGTTAGTCAAAT+TGG - chr6_2:76298437-76298456 Msa0929090:intron 35.0%
CACTTGGTCTCTAAATATGT+CGG + chr6_2:76298511-76298530 None:intergenic 35.0%
! CCATTTGAATGCTTTTGTTG+AGG + chr6_2:76298737-76298756 None:intergenic 35.0%
!! CCTCAACAAAAGCATTCAAA+TGG - chr6_2:76298734-76298753 Msa0929090:five_prime_UTR 35.0%
!! TGAGCTTTCACTTGTTTTTC+AGG - chr6_2:76298871-76298890 Msa0929090:five_prime_UTR 35.0%
TGGAAATGTGATTAATGGTG+GGG + chr6_2:76298957-76298976 None:intergenic 35.0%
GATGGAAATGTGATTAATGG+TGG + chr6_2:76298959-76298978 None:intergenic 35.0%
CCTGAATTATTGGTCATTGA+AGG + chr6_2:76299004-76299023 None:intergenic 35.0%
CCTTCAATGACCAATAATTC+AGG - chr6_2:76299001-76299020 Msa0929090:five_prime_UTR 35.0%
AAGAATTCCCAACTGAACTT+AGG + chr6_2:76299031-76299050 None:intergenic 35.0%
GAACTTGTGTGGATATGATT+TGG - chr6_2:76299080-76299099 Msa0929090:five_prime_UTR 35.0%
CAAAAATAGGCCCAAACATT+TGG + chr6_2:76299280-76299299 None:intergenic 35.0%
AACCGAAATCGATCAAGAAT+GGG - chr6_2:76299361-76299380 Msa0929090:five_prime_UTR 35.0%
! ATCCCATTCTTGATCGATTT+CGG + chr6_2:76299366-76299385 None:intergenic 35.0%
TTCTCTCTCTCTCGTAATTG+TGG - chr6_2:76296527-76296546 Msa0929090:CDS 40.0%
! AACATGTTCTAGCAGCCTTT+TGG - chr6_2:76296582-76296601 Msa0929090:CDS 40.0%
! TAGCAGCCTTTTGGTGAATT+GGG - chr6_2:76296591-76296610 Msa0929090:CDS 40.0%
GAAAGAAGATGTTGAGGATG+AGG - chr6_2:76296793-76296812 Msa0929090:CDS 40.0%
GTTTCTTCACCTATAAGCTC+AGG - chr6_2:76296852-76296871 Msa0929090:intron 40.0%
AGGGTTACGATTTGAATCAG+TGG + chr6_2:76296949-76296968 None:intergenic 40.0%
TTCAAATCGTAACCCTGCTT+TGG - chr6_2:76296953-76296972 Msa0929090:intron 40.0%
CTAATTGTAGGAACCAAAGC+AGG + chr6_2:76296969-76296988 None:intergenic 40.0%
TATAAAGCACGGACACTCTT+CGG - chr6_2:76297105-76297124 Msa0929090:intron 40.0%
!! TGTGATTTGGTGACATTGGT+TGG - chr6_2:76297427-76297446 Msa0929090:intron 40.0%
AAAATGTTGTCAGGACCAAC+TGG - chr6_2:76297469-76297488 Msa0929090:intron 40.0%
AGACAAAGAGAGGGATTCTA+AGG - chr6_2:76297525-76297544 Msa0929090:intron 40.0%
CGTATGACTCATACAATCTC+AGG + chr6_2:76297754-76297773 None:intergenic 40.0%
GTTGTAGAATCAGATGTGCT+TGG - chr6_2:76298180-76298199 Msa0929090:intron 40.0%
ACTAACGGAATACACGTCAA+AGG + chr6_2:76298432-76298451 None:intergenic 40.0%
CGACATATTTAGAGACCAAG+TGG - chr6_2:76298509-76298528 Msa0929090:CDS 40.0%
GCATTCAAATGGGAAACGAT+TGG - chr6_2:76298745-76298764 Msa0929090:five_prime_UTR 40.0%
GTAGTAACAACAACTCCTGA+GGG + chr6_2:76298911-76298930 None:intergenic 40.0%
TGTAGTAACAACAACTCCTG+AGG + chr6_2:76298912-76298931 None:intergenic 40.0%
GTTGTGGATGTAGATGTAGA+TGG + chr6_2:76298977-76298996 None:intergenic 40.0%
CTTAGGAGGACCTGAATTAT+TGG + chr6_2:76299014-76299033 None:intergenic 40.0%
AATTCCCAACTGAACTTAGG+AGG + chr6_2:76299028-76299047 None:intergenic 40.0%
ACAAGTTCAAACATGTGGCA+TGG + chr6_2:76299068-76299087 None:intergenic 40.0%
TCCACACAAGTTCAAACATG+TGG + chr6_2:76299073-76299092 None:intergenic 40.0%
! TTGATCGATTTCGGTTGGTT+GGG + chr6_2:76299357-76299376 None:intergenic 40.0%
! CATTCTTGATCGATTTCGGT+TGG + chr6_2:76299362-76299381 None:intergenic 40.0%
CAACCGAAATCGATCAAGAA+TGG - chr6_2:76299360-76299379 Msa0929090:five_prime_UTR 40.0%
GAAATCGATCAAGAATGGGA+TGG - chr6_2:76299365-76299384 Msa0929090:five_prime_UTR 40.0%
CTCTCGTAATTGTGGACTTG+TGG - chr6_2:76296535-76296554 Msa0929090:CDS 45.0%
ATTGTGGACTTGTGGAATGC+TGG - chr6_2:76296543-76296562 Msa0929090:CDS 45.0%
TTGTGGACTTGTGGAATGCT+GGG - chr6_2:76296544-76296563 Msa0929090:CDS 45.0%
! CTAGCAGCCTTTTGGTGAAT+TGG - chr6_2:76296590-76296609 Msa0929090:CDS 45.0%
! AGCCTTTTGGTGAATTGGGT+CGG - chr6_2:76296595-76296614 Msa0929090:CDS 45.0%
TCTTCACCTATAAGCTCAGG+TGG - chr6_2:76296855-76296874 Msa0929090:intron 45.0%
TGAACCGGGTTCAATGGAAT+GGG - chr6_2:76296887-76296906 Msa0929090:intron 45.0%
ATCTCCCATTCCATTGAACC+CGG + chr6_2:76296894-76296913 None:intergenic 45.0%
GTTCAATGGAATGGGAGATG+AGG - chr6_2:76296895-76296914 Msa0929090:intron 45.0%
GTTTGAACCAACATTCCACC+TGG + chr6_2:76296921-76296940 None:intergenic 45.0%
! AGAGTGTCCGTGCTTTATAG+CGG + chr6_2:76297104-76297123 None:intergenic 45.0%
ATTTGACACGTGTTCGACAC+CGG + chr6_2:76297143-76297162 None:intergenic 45.0%
ATGAAGCATGGATACGGACA+CGG + chr6_2:76297192-76297211 None:intergenic 45.0%
!! TGACATTGGTTGGTGTTGCA+GGG - chr6_2:76297437-76297456 Msa0929090:intron 45.0%
TTGGAAGTAGATAGGCTTGC+CGG - chr6_2:76297688-76297707 Msa0929090:intron 45.0%
TAACAACAACTCCTGAGGGT+TGG + chr6_2:76298907-76298926 None:intergenic 45.0%
GGTCATTGAAGGAGTAGTTG+TGG + chr6_2:76298993-76299012 None:intergenic 45.0%
AGGTCCTCCTAAGTTCAGTT+GGG - chr6_2:76299021-76299040 Msa0929090:five_prime_UTR 45.0%
GCCACATGTTTGAACTTGTG+TGG - chr6_2:76299069-76299088 Msa0929090:five_prime_UTR 45.0%
! TGATCGATTTCGGTTGGTTG+GGG + chr6_2:76299356-76299375 None:intergenic 45.0%
! CTTGATCGATTTCGGTTGGT+TGG + chr6_2:76299358-76299377 None:intergenic 45.0%
TGTGGACTTGTGGAATGCTG+GGG - chr6_2:76296545-76296564 Msa0929090:CDS 50.0%
CACCGACCCAATTCACCAAA+AGG + chr6_2:76296600-76296619 None:intergenic 50.0%
GAGAGAGGGTTAGATCTCTG+AGG + chr6_2:76296656-76296675 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr6_2:76296479-76296498 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr6_2:76296480-76296499 None:intergenic 50.0%
CTGCAACCACCTGAGCTTAT+AGG + chr6_2:76296864-76296883 None:intergenic 50.0%
CTGAACCGGGTTCAATGGAA+TGG - chr6_2:76296886-76296905 Msa0929090:intron 50.0%
! TCTTCGGATTAGGCGTGTTC+CGG - chr6_2:76297121-76297140 Msa0929090:intron 50.0%
!! GTGACATTGGTTGGTGTTGC+AGG - chr6_2:76297436-76297455 Msa0929090:intron 50.0%
!! GACATTGGTTGGTGTTGCAG+GGG - chr6_2:76297438-76297457 Msa0929090:intron 50.0%
AAGTAGATAGGCTTGCCGGA+CGG - chr6_2:76297692-76297711 Msa0929090:intron 50.0%
ATACACGTCAAAGGACCGCA+TGG + chr6_2:76298423-76298442 None:intergenic 50.0%
GAACAACAACACCAACCCTC+AGG - chr6_2:76298893-76298912 Msa0929090:five_prime_UTR 50.0%
CAGGTCCTCCTAAGTTCAGT+TGG - chr6_2:76299020-76299039 Msa0929090:five_prime_UTR 50.0%
TTGTGGAATGCTGGGGTCAG+AGG - chr6_2:76296552-76296571 Msa0929090:CDS 55.0%
AGGTGGTTGCAGAGCTGAAC+CGG - chr6_2:76296872-76296891 Msa0929090:intron 55.0%
CAGAGCTGAACCGGGTTCAA+TGG - chr6_2:76296881-76296900 Msa0929090:intron 55.0%
ATGGAATGGGAGATGAGGCC+AGG - chr6_2:76296900-76296919 Msa0929090:intron 55.0%
GATGAGGCCAGGTGGAATGT+TGG - chr6_2:76296911-76296930 Msa0929090:intron 55.0%
GCACGGACACTCTTCGGATT+AGG - chr6_2:76297111-76297130 Msa0929090:intron 55.0%
AGTAGATAGGCTTGCCGGAC+GGG - chr6_2:76297693-76297712 Msa0929090:intron 55.0%
GACGTAAACACGTACCCGTC+CGG + chr6_2:76297710-76297729 None:intergenic 55.0%
GGTGGTTGCAGAGCTGAACC+GGG - chr6_2:76296873-76296892 Msa0929090:intron 60.0%
GAATGGGAGATGAGGCCAGG+TGG - chr6_2:76296903-76296922 Msa0929090:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 76296106 76299397 76296106 ID=Msa0929090;Name=Msa0929090
chr6_2 mRNA 76296106 76299397 76296106 ID=Msa0929090-mRNA-1;Parent=Msa0929090;Name=Msa0929090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=695|1|1|1|1|1|4|340|356
chr6_2 exon 76296106 76296862 76296106 ID=Msa0929090-mRNA-1:exon:12874;Parent=Msa0929090-mRNA-1
chr6_2 exon 76297216 76297362 76297216 ID=Msa0929090-mRNA-1:exon:12873;Parent=Msa0929090-mRNA-1
chr6_2 exon 76297789 76298045 76297789 ID=Msa0929090-mRNA-1:exon:12872;Parent=Msa0929090-mRNA-1
chr6_2 exon 76298453 76299397 76298453 ID=Msa0929090-mRNA-1:exon:12871;Parent=Msa0929090-mRNA-1
chr6_2 five_prime_UTR 76298703 76299397 76298703 ID=Msa0929090-mRNA-1:five_prime_utr;Parent=Msa0929090-mRNA-1
chr6_2 CDS 76298453 76298702 76298453 ID=Msa0929090-mRNA-1:cds;Parent=Msa0929090-mRNA-1
chr6_2 CDS 76297789 76298045 76297789 ID=Msa0929090-mRNA-1:cds;Parent=Msa0929090-mRNA-1
chr6_2 CDS 76297216 76297362 76297216 ID=Msa0929090-mRNA-1:cds;Parent=Msa0929090-mRNA-1
chr6_2 CDS 76296446 76296862 76296446 ID=Msa0929090-mRNA-1:cds;Parent=Msa0929090-mRNA-1
chr6_2 three_prime_UTR 76296106 76296445 76296106 ID=Msa0929090-mRNA-1:three_prime_utr;Parent=Msa0929090-mRNA-1
Gene Sequence

>Msa0929090

ATGTTGAGGATGAGGAATAATCATAATCATGGTAATAATAAGACAAATGGTGTTTCTTCACCTATAAGCTCAGGTGGTTGCAGAGCTGAACCGGGTTCAATGGAATGGGAGATGAGGCCAGGTGGAATGTTGGTTCAAACAAGAACCACTGATTCAAATCGTAACCCTGCTTTGGTTCCTACAATTAGAGTTAGGGTCAAGTTTGGTTCAATTTATCATGAAGTCAATATTAGTTCACAAGCCACATTTGGGGATTTGAAGAAAATGTTGTCAGGACCAACTGGTTTACATCATCAAGATCAAAAGTTGTTTTACAAAGACAAAGAGAGGGATTCTAAGGCTTTTCTTGATGTAGTTGGTGTAAAGGACAAATCTAAACTAGTTCTTGTGGAAGATCCTATTAGTCAAGAGAAAAGGATTTTGGAATTGAGGAAGAATGCTAAGATGGAGAAAGCTGCAAAATCTATCTCACAGATTAGCTTGGAAGTAGATAGGCTTGCCGGACGGGTATCTGCTTTTGAATCAATAATTAGCAAAGGTGGGAAAGTTGTAGAATCAGATGTGCTTGGTCTTATTGAGCTATTGATGAATCAATTGCTTAAATTGGATGGTATAATTACTGATGGCGATGTCAAATTGCAAAGAAAAATGCAGGTAAGGAGAGTTCAAAAGTATGTTGAAACATTGGATATGCTAAAAGCTAAGAATTCTATACAAAATGGCAATGATGTTCATGCTCAAGTGCAACCTCAACAAAAGCATTCAAATGGGAAACGATTGGAAACAATTCAAGAGCAACCACAACAAATTCATTCAAATGGTTATCATGGGTCAATAGTACCAATTCAAGAACAACAAGAAGAACAACCTAGAAAAAACTCAAATGAGCTTTCACTTGTTTTTCAGGAACAACAACACCAACCCTCAGGAGTTGTTGTTACTACAAAATGGGAGACATTTGATTCTACCCCACCATTAATCACATTTCCATCTACATCTACATCCACAACTACTCCTTCAATGACCAATAATTCAGGTCCTCCTAAGTTCAGTTGGGAATTCTTTGAGTGA
Protein sequence

>Msa0929090

MLRMRNNHNHGNNKTNGVSSPISSGGCRAEPGSMEWEMRPGGMLVQTRTTDSNRNPALVPTIRVRVKFGSIYHEVNISSQATFGDLKKMLSGPTGLHHQDQKLFYKDKERDSKAFLDVVGVKDKSKLVLVEDPISQEKRILELRKNAKMEKAAKSISQISLEVDRLAGRVSAFESIISKGGKVVESDVLGLIELLMNQLLKLDGIITDGDVKLQRKMQVRRVQKYVETLDMLKAKNSIQNGNDVHAQVQPQQKHSNGKRLETIQEQPQQIHSNGYHGSIVPIQEQQEEQPRKNSNELSLVFQEQQHQPSGVVVTTKWETFDSTPPLITFPSTSTSTTTPSMTNNSGPPKFSWEFFE*