AlfalfaGEDB | Msa0191250

M. sativa cultivar zhongmu-4 / Msa0191250

KEGG: K15601
GO: GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF08879.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191250	XP_013462466.1	96.369	964	29	4	1	959	1	963	0.0	1911

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191250	sp\|Q9SSE9\|JMJ25_ARATH	35.171	526	266	10	435	949	429	890	5.45e-86	301
Msa0191250	sp\|Q9SSE9\|JMJ25_ARATH	31.515	165	110	3	205	368	155	317	2.37e-17	91.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191250	A0A072V3U7	96.369	964	29	4	1	959	1	963	0.0	1911

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TFs/TRs:

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Gene ID	Type	Classification
Msa0191250	TR	Jumonji

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa0191250	-0.873789	1.119977e-67	-8.615850e-47
Msa0004880	Msa0191250	0.812972	3.239955e-51	-8.615850e-47
Msa0004920	Msa0191250	0.823013	1.740503e-53	-8.615850e-47
Msa0006110	Msa0191250	0.813862	2.064701e-51	-8.615850e-47
Msa0007080	Msa0191250	0.850774	1.365935e-60	-8.615850e-47
Msa0008670	Msa0191250	0.809482	1.851248e-50	-8.615850e-47
Msa0010020	Msa0191250	0.800760	1.239279e-48	-8.615850e-47
Msa0011500	Msa0191250	0.812068	5.106560e-51	-8.615850e-47
Msa0012060	Msa0191250	0.815442	9.227568e-52	-8.615850e-47
Msa0012550	Msa0191250	0.801749	7.772529e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191250	MtrunA17_Chr2g0281051	96.369	964	29	4	1	959	1	963	0.0	1911
Msa0191250	MtrunA17_Chr4g0048531	63.978	558	171	7	423	953	725	1279	0.0	714
Msa0191250	MtrunA17_Chr4g0048531	50.000	508	180	12	1	442	1	500	6.14e-127	415
Msa0191250	MtrunA17_Chr7g0275771	35.951	815	443	20	198	953	133	927	2.98e-156	484
Msa0191250	MtrunA17_Chr1g0192011	30.372	968	564	25	12	957	88	967	2.56e-134	428
Msa0191250	MtrunA17_Chr1g0211711	34.980	749	425	15	205	942	343	1040	9.67e-131	421
Msa0191250	MtrunA17_Chr4g0075261	33.460	792	439	18	180	952	144	866	5.05e-128	408
Msa0191250	MtrunA17_Chr1g0183311	35.045	779	440	17	205	955	144	884	4.65e-121	390
Msa0191250	MtrunA17_Chr2g0289121	32.938	759	403	18	203	949	154	818	2.13e-105	347
Msa0191250	MtrunA17_Chr5g0420361	35.441	680	378	22	287	949	157	792	5.27e-99	329

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191250	AT4G21430.1	38.454	996	487	23	6	955	2	917	0.0	619
Msa0191250	AT4G00990.1	36.030	791	438	19	198	952	73	831	2.92e-149	463
Msa0191250	AT1G62310.1	35.316	790	413	17	170	939	172	883	2.03e-137	434
Msa0191250	AT4G00990.2	35.479	730	403	19	198	891	73	770	2.34e-128	407
Msa0191250	AT4G00990.3	35.479	730	403	19	198	891	73	770	2.85e-128	406
Msa0191250	AT1G09060.4	29.877	974	554	28	12	957	39	911	6.47e-117	381
Msa0191250	AT1G09060.3	29.877	974	554	28	12	957	39	911	6.47e-117	381
Msa0191250	AT1G09060.2	29.877	974	554	28	12	957	25	897	6.70e-117	380
Msa0191250	AT1G09060.1	29.877	974	554	28	12	957	25	897	6.70e-117	380
Msa0191250	AT1G11950.2	34.686	542	330	11	205	740	193	716	3.80e-87	297

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Find 174 sgRNAs with CRISPR-Local

Find 322 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAATACATGTTCTGG+AGG	0.643807	2_1:+6804487	None:intergenic
AAAACTAAGTTGGGATCCTC+TGG	0.488482	2_1:-6807197	Msa0191250:CDS
AAACTCGTAAAGATCTAGCA+AGG	0.635952	2_1:-6804445	Msa0191250:three_prime_UTR
AAACTGTTCTTTGAATAGTT+GGG	0.497020	2_1:+6806718	None:intergenic
AAATGCTCTTGTCTTCTAAA+AGG	0.324737	2_1:+6807644	None:intergenic
AAATGTATTCTGTTGCTCAG+TGG	0.670307	2_1:-6805504	Msa0191250:CDS
AAATTCAGAAGACCGAACCT+CGG	0.675191	2_1:+6806641	None:intergenic
AACCACCTAGCTCTGAAGGA+GGG	0.641948	2_1:+6807545	None:intergenic
AACGAAGGAAGAATGTCCAC+CGG	0.642524	2_1:-6809790	Msa0191250:CDS
AACTGATACGTCAATGTTGC+TGG	0.394642	2_1:-6807608	Msa0191250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCATCTTCAACTTCAACAT+CGG	-	chr2_1:6804658-6804677	Msa0191250:intron	30.0%
GCAATTGGATTTGATAAGAA+TGG	-	chr2_1:6804824-6804843	Msa0191250:CDS	30.0%
TTCTGCACATTCTTCTTATT+CGG	+	chr2_1:6804882-6804901	None:intergenic	30.0%
GTGAAAGTGAATTATAGTGA+AGG	-	chr2_1:6804924-6804943	Msa0191250:intron	30.0%
ATTATAGTGAAGGTGAATTG+AGG	-	chr2_1:6804934-6804953	Msa0191250:intron	30.0%
CCCACTTGAAAATTATCATA+AGG	+	chr2_1:6805107-6805126	None:intergenic	30.0%
TGTTGTTGTGTACTAATGTT+TGG	-	chr2_1:6805180-6805199	Msa0191250:intron	30.0%
TATTGTCTGTTGTGCATATA+TGG	-	chr2_1:6805424-6805443	Msa0191250:CDS	30.0%
TATAGAAGTTATCATGGAGT+TGG	+	chr2_1:6805800-6805819	None:intergenic	30.0%
AACTGGTATAGAAGTTATCA+TGG	+	chr2_1:6805806-6805825	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	6804334	6810029	6804334	ID=Msa0191250;Name=Msa0191250
chr2_1	mRNA	6804334	6810029	6804334	ID=Msa0191250-mRNA-1;Parent=Msa0191250;Name=Msa0191250-mRNA-1;_AED=0.09;_eAED=0.09;_QI=203\|1\|1\|1\|1\|1\|12\|201\|959
chr2_1	exon	6804334	6804615	6804334	ID=Msa0191250-mRNA-1:exon:3121;Parent=Msa0191250-mRNA-1
chr2_1	exon	6804734	6804853	6804734	ID=Msa0191250-mRNA-1:exon:3120;Parent=Msa0191250-mRNA-1
chr2_1	exon	6805079	6805164	6805079	ID=Msa0191250-mRNA-1:exon:3119;Parent=Msa0191250-mRNA-1
chr2_1	exon	6805250	6805322	6805250	ID=Msa0191250-mRNA-1:exon:3118;Parent=Msa0191250-mRNA-1
chr2_1	exon	6805408	6805863	6805408	ID=Msa0191250-mRNA-1:exon:3117;Parent=Msa0191250-mRNA-1
chr2_1	exon	6806514	6806840	6806514	ID=Msa0191250-mRNA-1:exon:3116;Parent=Msa0191250-mRNA-1
chr2_1	exon	6807111	6807840	6807111	ID=Msa0191250-mRNA-1:exon:3115;Parent=Msa0191250-mRNA-1
chr2_1	exon	6807933	6807996	6807933	ID=Msa0191250-mRNA-1:exon:3114;Parent=Msa0191250-mRNA-1
chr2_1	exon	6808086	6808218	6808086	ID=Msa0191250-mRNA-1:exon:3113;Parent=Msa0191250-mRNA-1
chr2_1	exon	6808313	6808418	6808313	ID=Msa0191250-mRNA-1:exon:3112;Parent=Msa0191250-mRNA-1
chr2_1	exon	6809006	6809124	6809006	ID=Msa0191250-mRNA-1:exon:3111;Parent=Msa0191250-mRNA-1
chr2_1	exon	6809242	6810029	6809242	ID=Msa0191250-mRNA-1:exon:3110;Parent=Msa0191250-mRNA-1
chr2_1	five_prime_UTR	6809827	6810029	6809827	ID=Msa0191250-mRNA-1:five_prime_utr;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6809242	6809826	6809242	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6809006	6809124	6809006	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6808313	6808418	6808313	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6808086	6808218	6808086	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6807933	6807996	6807933	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6807111	6807840	6807111	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6806514	6806840	6806514	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6805408	6805863	6805408	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6805250	6805322	6805250	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6805079	6805164	6805079	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6804734	6804853	6804734	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	CDS	6804535	6804615	6804535	ID=Msa0191250-mRNA-1:cds;Parent=Msa0191250-mRNA-1
chr2_1	three_prime_UTR	6804334	6804534	6804334	ID=Msa0191250-mRNA-1:three_prime_utr;Parent=Msa0191250-mRNA-1

Gene Sequence

>Msa0191250

ATGGTGAAAAAATCAACGAAGGAAGAATGTCCACCGGACCATCTCCGGTGCAGCCGTACCGACGGCCGACAATGGCGGTGCAAACGTCGAGCGATGGAAAACGTGAAGTTATGCGAAGTTCATCATCTTCAACTTCAACATCGGCAGAAGAAAGTGAAGGTTCCAGAGTCGTTGAAGCTTCAGAGAAATATGAAGATGAAGAAGAAGAAGCAAAACGACGTCGTTGAGGTTGAAATTAGGTCAAAGAAGAAAAAGAAGAAGAGTAAGAAAAACGATGCTGTTGATGTGCAATTGGATTTGATAAGAATGGTGTTACAGAGAGAGGTTGAGAAGAAGAAGAATCCGAATAAGAAGAATGTGCAGAATGTTGATGAAGAATTGGAATTGGTGAAAGTGAATTATAGTGAAGGTGAATTGAGGAAAGAATTGCCCAATGGGGTTATGGAAATTGCTCCTGTTTCGACTTCACGTCGTGATGATGATGATGATGTTGTTGATTTTCACTGTAATGGTAAAGCTGTTGTTACACCACCTTACTTCAGGTCCAAGAATGTTGATAGAAGGGTCCCTTATGATAATTTTCAAGTTTTGAAGAAGTGTAGGAGGAAGAAGTGTCATTGGTGTCAGAGTAGTGATTCTTTGAATTTGATCAAGTGTAACAACTGCCAGAAAGAGTTTTTCTGCATGGATTGCATTAAACAACGGTATTTCGACACTCAAAATGAGGTTAAGAAGGCGTGTCCAGTTTGTCGAGGAACTTGTTCATGTAAAGATTGCCGAGCAAGTCAGTGTAAAGACCGTGAAAGTAAGGATTGTTTGGCTGGTACAAGCAGAGTTGATAGGATATTGCATTTTCATTATTTGGTCTGTATGCTCCTTCCGGTAATAAAACAAATAAGCGAAGACCAGCATGCAGAGCTAGAAACAGAAGCGAAAAATAAAGGGGAAAGTATTTCTGATATTCTCATCAAGCAAATCGAATTTGACTGCAATGAGAAAATTGACTGCAACTACTGCAAAACACCTATTCTGAATCTTCACAGAAGCTGCCTTAACTGTTCCTACAGTCTATGCTTAAGATGTTGTCAGACATTAAGTCAAGGAAGCCCCTTTGAGCATATAAATTCTCCATTGACTGAGTTACCTGACAAAATGGACACTTGCATTGCTGATGAAAGTTGCCTTTTAGAAGACAAGAGCATTTCTAGCGACAATGAAACTGATACGTCAATGTTGCTGGATTCGACTGGTTTTAACGGTACAACTGACAGTATATCATGCCCTCCTTCAGAGCTAGGTGGTTGTGGTAATGACAACCTTGATTTGAGATGTGTTTTTCCGATAAGCTGGATCGAAGAAATGGAAGCTAAGGCAGAAGAGATAGTTTGCAGCTATGAAGTTCCTGAAATTTTGGATAAAAACTCAAGTTGCTCACTTTGTATTGACAAAGATCATAAAACTAAGAGACATAAGCAATTTGAAGATGCTGCTCGAAGAGAAGATTCTAATGATAATTGCTTATTTTATCCTACAGTTTTCGACATCAATTGCGATCATTTTGAACACTTTCAAAAACACTGGGGGAAAGGCCATCCTGTAGTAGTCCGAGACGTGCTCCGAAGTACGAAAAAACTAAGTTGGGATCCTCTGGTCTTGTTCTGTGCTTATCTTGAAAGGAGCATTGCAAGATATGAGAATAATAAAGACTTACTTGAAGCTTGTTTGGATTGGTGGGAGGTTGAAATTAACATTAGACAGCATTTTACTGGCTCTCTTAAATATCAACCTCGGAAAAATGATTGGCATGAGACGCTGAAACTAAATGGATGGTTATCTTCCCAACTATTCAAAGAACAGTTTCCAGCTCATTTTGCTGAAGTAATTGGTGCTCTTCCACTTCAAGAATACATGAATCCGAGGTTCGGTCTTCTGAATTTAGCTGCAAATATGACAGAGGGGAGAGCAGCAATACATGATAAAGGCCCATATGTATACATTTCTTATGGCTGTGCTGACAACAAAGCTTATTCTGTGCTAAATCTCTCTTATGACTCGTATGATGTGGTTAATATTATGGTTTATACCACAGATGTTCCTGTCTCTACAGAACATCTTACTAAAATAACTAAGTTATTGAAGAAGCACAGAACTCTGTGTCAAAGGGGGTCACCGACTGAAAATGCAGAAGACGCGGAACAAAAGGAACCAGAGAGTATTGTAAGCGAAGGAACCGACTTTTACAGGAGAGTCAATAGAACATCTTACATCTCTACTGAAGTGAAAGCAATAGCTAGTCAAAGTTTAGAGAACAATACTTCCAGCAATGGAGAATGTGGTTCTGGTTCTGATGCTGAAAAAACAGAAAGCTCTTTACCTTTCCACAAGACAGATCAAAGCACTGAAATGTATTCTGTTGCTCAGTGGGATGTCTTCCGGAGACAGGATGTACCGAAGCTTTTAGAATACCTCAAAAGACACAGGGATGAATTTTCTTTTACCAACGAGTATGACAGCAATAAGATAGTTCACCCAATTCTAGATCAGAGCATCTTTCTGGACAATACTCACAAAATGAGACTGAAGGAGGAGTTTGAAATCGAACCATGGACGTTTGAGCAACATGTTGGAGAAGCTATCATCATTCCTGCTGGATGTCCATACCAGATTAGGAATTCTAAGTATTGTGTTCAAGTGGTATTGGAATTTATGTCTTCCGAGAGTGTTGCTGAGTGTATCCAATTGACCGATGAGATCCATCTGCTTCCAGAGAACCACAAAGCAAAAGTAGACAAGATTGAGGTGAAGAAAATGGCTCTTCATAGCATGGATATGGCGATAAAAGAAATACGCGAGCTCACTTCCAATCCAAAACATGATTGA

Protein sequence

>Msa0191250

MVKKSTKEECPPDHLRCSRTDGRQWRCKRRAMENVKLCEVHHLQLQHRQKKVKVPESLKLQRNMKMKKKKQNDVVEVEIRSKKKKKKSKKNDAVDVQLDLIRMVLQREVEKKKNPNKKNVQNVDEELELVKVNYSEGELRKELPNGVMEIAPVSTSRRDDDDDVVDFHCNGKAVVTPPYFRSKNVDRRVPYDNFQVLKKCRRKKCHWCQSSDSLNLIKCNNCQKEFFCMDCIKQRYFDTQNEVKKACPVCRGTCSCKDCRASQCKDRESKDCLAGTSRVDRILHFHYLVCMLLPVIKQISEDQHAELETEAKNKGESISDILIKQIEFDCNEKIDCNYCKTPILNLHRSCLNCSYSLCLRCCQTLSQGSPFEHINSPLTELPDKMDTCIADESCLLEDKSISSDNETDTSMLLDSTGFNGTTDSISCPPSELGGCGNDNLDLRCVFPISWIEEMEAKAEEIVCSYEVPEILDKNSSCSLCIDKDHKTKRHKQFEDAARREDSNDNCLFYPTVFDINCDHFEHFQKHWGKGHPVVVRDVLRSTKKLSWDPLVLFCAYLERSIARYENNKDLLEACLDWWEVEINIRQHFTGSLKYQPRKNDWHETLKLNGWLSSQLFKEQFPAHFAEVIGALPLQEYMNPRFGLLNLAANMTEGRAAIHDKGPYVYISYGCADNKAYSVLNLSYDSYDVVNIMVYTTDVPVSTEHLTKITKLLKKHRTLCQRGSPTENAEDAEQKEPESIVSEGTDFYRRVNRTSYISTEVKAIASQSLENNTSSNGECGSGSDAEKTESSLPFHKTDQSTEMYSVAQWDVFRRQDVPKLLEYLKRHRDEFSFTNEYDSNKIVHPILDQSIFLDNTHKMRLKEEFEIEPWTFEQHVGEAIIIPAGCPYQIRNSKYCVQVVLEFMSSESVAECIQLTDEIHLLPENHKAKVDKIEVKKMALHSMDMAIKEIRELTSNPKHD*