AlfalfaGEDB | Msa1029520

M. sativa cultivar zhongmu-4 / Msa1029520

KEGG: K11820|K13691|K21374
GO: GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006979 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009072 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009696 GO:0009719 GO:0009725 GO:0009737 GO:0009850 GO:0009987 GO:0009991 GO:0010016 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010817 GO:0016740 GO:0016757 GO:0016758 GO:0016999 GO:0017144 GO:0018874 GO:0018958 GO:0019752 GO:0031668 GO:0032501 GO:0032502 GO:0032787 GO:0032870 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0034641 GO:0035251 GO:0035690 GO:0042221 GO:0042430 GO:0042445 GO:0042493 GO:0042537 GO:0042538 GO:0042542 GO:0042631 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046482 GO:0046483 GO:0046527 GO:0046677 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051716 GO:0052638 GO:0052639 GO:0052640 GO:0052641 GO:0065007 GO:0065008 GO:0070301 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071236 GO:0071310 GO:0071396 GO:0071462 GO:0071470 GO:0071472 GO:0071474 GO:0071475 GO:0071495 GO:0071496 GO:0071704 GO:0080002 GO:0080024 GO:0080043 GO:0080044 GO:0080167 GO:0090704 GO:0097237 GO:0097305 GO:0097306 GO:0104004 GO:1900140 GO:1901360 GO:1901564 GO:1901615 GO:1901700 GO:1901701 GO:2000026
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1029520	XP_003624245.2	90.196	459	32	3	1	447	29	486	0.0	853

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1029520	sp\|K7NBW3\|74AC1_SIRGR	43.805	452	236	8	11	447	6	454	6.11e-123	368

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1029520	A0A396H1P4	90.196	459	32	3	1	447	29	486	0.0	853

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010800	Msa1029520	0.823439	1.384072e-53	-8.615850e-47
Msa0043850	Msa1029520	0.833476	5.218388e-56	-8.615850e-47
Msa0137980	Msa1029520	0.833109	6.440287e-56	-8.615850e-47
Msa0138180	Msa1029520	0.811018	8.636570e-51	-8.615850e-47
Msa0276430	Msa1029520	0.839087	1.954261e-57	-8.615850e-47
Msa0316470	Msa1029520	0.827777	1.297431e-54	-8.615850e-47
Msa0362160	Msa1029520	0.807109	5.935087e-50	-8.615850e-47
Msa0365950	Msa1029520	0.802245	6.147620e-49	-8.615850e-47
Msa0456770	Msa1029520	0.815772	7.789272e-52	-8.615850e-47
Msa0713060	Msa1029520	0.801091	1.060333e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1029520	MtrunA17_Chr7g0249931	90.196	459	32	3	1	447	29	486	0.0	853
Msa1029520	MtrunA17_Chr7g0250001	63.853	462	144	8	1	449	1	452	0.0	578
Msa1029520	MtrunA17_Chr7g0249981	61.707	457	157	8	1	444	15	466	0.0	560
Msa1029520	MtrunA17_Chr7g0250021	81.651	327	47	3	1	315	1	326	0.0	547
Msa1029520	MtrunA17_Chr6g0464871	54.767	451	185	8	11	446	22	468	1.02e-174	498
Msa1029520	MtrunA17_Chr6g0464881	51.535	456	202	8	4	444	3	454	3.90e-168	481
Msa1029520	MtrunA17_Chr6g0464841	53.111	450	192	8	12	446	11	456	7.79e-168	479
Msa1029520	MtrunA17_Chr6g0464861	52.116	449	197	7	12	446	11	455	4.08e-167	478
Msa1029520	MtrunA17_Chr6g0464921	52.174	460	199	10	1	444	1	455	2.64e-165	473
Msa1029520	MtrunA17_Chr5g0414331	44.444	450	228	10	10	444	11	453	4.95e-126	374

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1029520	AT1G05675.1	41.648	449	240	9	12	442	6	450	2.91e-110	333
Msa1029520	AT1G05680.1	40.757	449	244	9	12	442	6	450	2.64e-109	330
Msa1029520	AT2G31750.1	41.538	455	240	10	12	446	8	456	2.52e-108	328
Msa1029520	AT2G31750.2	41.447	456	241	10	11	446	7	456	9.31e-108	328
Msa1029520	AT2G31790.1	39.479	461	247	10	8	444	4	456	2.38e-106	323
Msa1029520	AT2G43840.2	39.686	446	250	11	12	444	7	446	2.36e-105	320
Msa1029520	AT2G43820.1	39.602	452	248	12	9	444	4	446	2.52e-105	320
Msa1029520	AT2G43840.1	39.462	446	251	11	12	444	7	446	1.98e-104	318
Msa1029520	AT1G24100.1	39.869	459	239	16	12	444	11	458	1.44e-95	295
Msa1029520	AT4G15480.1	33.192	473	273	12	7	444	14	478	3.04e-77	249

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Find 90 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGTATATGGTAGGCA+AGG	0.589910	7_1:+62949037	None:intergenic
AAAGTGAAGAAAGTATTAAA+TGG	0.366602	7_1:-62948967	Msa1029520:CDS
AACCTCCCTTTGCTGAGGAA+AGG	0.512130	7_1:-62950022	Msa1029520:CDS
AATTGTATTTGAATAAATTT+TGG	0.160289	7_1:-62950247	Msa1029520:CDS
ACAATTGAGACCATTTCTGA+TGG	0.581663	7_1:-62950308	Msa1029520:intron
ACATTGGAAGCTTTGAGTAT+AGG	0.385135	7_1:-62948674	Msa1029520:CDS
ACTCAATGAGGAACAAATAG+AGG	0.483111	7_1:-62948888	Msa1029520:CDS
ACTTCCTCAACTACAACCTA+GGG	0.613689	7_1:-62949985	Msa1029520:CDS
ACTTTGGCTGCAAGAGCGGT+TGG	0.544404	7_1:-62948449	Msa1029520:CDS
AGAAATGGTCTCAATTGTAA+TGG	0.467504	7_1:+62950313	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAATGGTCTCAATTGTAA+TGG	+	chr7_1:62948560-62948579	None:intergenic	30.0%
TTTGTCAAAACCATCAGAAA+TGG	+	chr7_1:62948575-62948594	None:intergenic	30.0%
TTTATGATTCATTCATGCCT+TGG	-	chr7_1:62948722-62948741	Msa1029520:CDS	30.0%
TCATAGGTAAAGTAGAAAGA+AGG	+	chr7_1:62948916-62948935	None:intergenic	30.0%
ATTCCCATATCAAGAAAAGT+TGG	+	chr7_1:62948946-62948965	None:intergenic	30.0%
CTAATATTCACAAAGCTGAT+TGG	-	chr7_1:62948977-62948996	Msa1029520:CDS	30.0%
GAAAGAGGTAAGTAAGAATT+TGG	-	chr7_1:62949029-62949048	Msa1029520:CDS	30.0%
AAAATCAAATGGTTGTGGTT+TGG	+	chr7_1:62949085-62949104	None:intergenic	30.0%
ACATGAAAATCAAATGGTTG+TGG	+	chr7_1:62949090-62949109	None:intergenic	30.0%
CGTACAAATATCATTCATCA+AGG	+	chr7_1:62949130-62949149	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	62948376	62950516	62948376	ID=Msa1029520;Name=Msa1029520
chr7_1	mRNA	62948376	62950516	62948376	ID=Msa1029520-mRNA-1;Parent=Msa1029520;Name=Msa1029520-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0.33\|0\|1\|0.33\|0.25\|4\|0\|451
chr7_1	exon	62950309	62950516	62950309	ID=Msa1029520-mRNA-1:exon:13578;Parent=Msa1029520-mRNA-1
chr7_1	exon	62950078	62950296	62950078	ID=Msa1029520-mRNA-1:exon:13577;Parent=Msa1029520-mRNA-1
chr7_1	exon	62949857	62950053	62949857	ID=Msa1029520-mRNA-1:exon:13576;Parent=Msa1029520-mRNA-1
chr7_1	exon	62948376	62949107	62948376	ID=Msa1029520-mRNA-1:exon:13575;Parent=Msa1029520-mRNA-1
chr7_1	CDS	62950309	62950516	62950309	ID=Msa1029520-mRNA-1:cds;Parent=Msa1029520-mRNA-1
chr7_1	CDS	62950078	62950296	62950078	ID=Msa1029520-mRNA-1:cds;Parent=Msa1029520-mRNA-1
chr7_1	CDS	62949857	62950053	62949857	ID=Msa1029520-mRNA-1:cds;Parent=Msa1029520-mRNA-1
chr7_1	CDS	62948376	62949107	62948376	ID=Msa1029520-mRNA-1:cds;Parent=Msa1029520-mRNA-1

Gene Sequence

>Msa1029520

ATGGAGAACAAAACCATATCCACCAAGAGTGTCCATTGTTTGGTGTTGCCATTTCCAGCACATGGTCACACTAATCCAATGCTAGAATTCTCCAAGCTTTTACAACAACAACAAGGAGTCAAAGTAACACTTGTCACCACCCTTTCCTACTACAAAAACATACCTAAATTGCCAAATAATTCCATTACAATTGAGACCATTTCTGATGGTGGAGTTGCTGAAGCAAAGGATTTTAAATTGTATTTGAATAAATTTTGGCAAGTTGGTCCACAATCTCTTGCTGATCTTATTAACAACCTTAACGCTAGATCAAACGACCATGTTGATTGTCTTATTTATGATTCATTCATGCCTTGGTGCTTAGATGTTGCAAAGAAATTTGGAATTGTTGGTGCTTCTTTTCTAACTCAAAATCTTGTTATGAATAGAAAGTTGAAACCTCCCTTTGCTGAGGAAAGGATTACTCTTCCTGCACTTCCTCAACTACAACCTAGGGACATGCCTTCTTTCTACTTTACCTATGAAAAAGATCCAACTTTTCTTGATATGGGAATGGCGCAGTTCTCTAATATTCACAAAGCTGATTGGATCCTTGGCAATTCCTTCTTTGAGCTGGAGAAAGAGGTGGCTGATTGGACAATGAAGATTTGGTCCAACTTTAGGACCATAGGGCCTTGCCTACCATATACAGTTTTAGATAAGCGAATTAGAGATGATGAAGATTATGGTATTGTACAATCAAAAAGTGAAGAAAGTATTAAATGGCTAAATAATAAGCCAAAAAGGTCTGTTGTTTATGTTTCATTTGGGAGTATGGCATCACTCAATGAGGAACAAATAGAGGAAGTAGCACACTGTTTGAAAGATTGTGGAAGTTACTTTTTATGGGTAGTGAAAACCTCTGAAGAGACTAAGCTCCCAAAAAACTTTGAAAAGAAATCAGAGAATGGCTTAGTTGTGACATGGTGCCCCCAGTTAGAGGTTCTAGCTCATGAAGCAATAGGGTGTTTTGTAACACATTGTGGTTGGAATTCCACATTGGAAGCTTTGAGTATAGGAGTTCCAATTGTTGCAATGCCACTTTACTCAGACCAAGGCATTGATGCAAAATTTCTTGTAGATGTTTGGAAAGTGGGAATTAGACCTTTAGTTGATGAGAAACAAATAGTGAGAAAAGATACATTGAAGGATTGTATATGTGAAATAATGGATATGAGTGAAAAAGGCAAAGAGATTATGAATAATGTCATGCACTGGAAGACTTTGGCTGCAAGAGCGGTTGGTAAAGATGGCAGTTCTCATAAAAACATGATAGAATTTGTGAATAGCTTGTTCCAAGTTTCAACCACAAATTAA

Protein sequence

>Msa1029520

MENKTISTKSVHCLVLPFPAHGHTNPMLEFSKLLQQQQGVKVTLVTTLSYYKNIPKLPNNSITIETISDGGVAEAKDFKLYLNKFWQVGPQSLADLINNLNARSNDHVDCLIYDSFMPWCLDVAKKFGIVGASFLTQNLVMNRKLKPPFAEERITLPALPQLQPRDMPSFYFTYEKDPTFLDMGMAQFSNIHKADWILGNSFFELEKEVADWTMKIWSNFRTIGPCLPYTVLDKRIRDDEDYGIVQSKSEESIKWLNNKPKRSVVYVSFGSMASLNEEQIEEVAHCLKDCGSYFLWVVKTSEETKLPKNFEKKSENGLVVTWCPQLEVLAHEAIGCFVTHCGWNSTLEALSIGVPIVAMPLYSDQGIDAKFLVDVWKVGIRPLVDEKQIVRKDTLKDCICEIMDMSEKGKEIMNNVMHWKTLAARAVGKDGSSHKNMIEFVNSLFQVSTTN*