AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1109810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1109810 MtrunA17_Chr7g0244371 93.130 131 9 0 6 136 82 212 3.77e-90 260
Msa1109810 MtrunA17_Chr7g0244341 85.484 124 18 0 12 135 90 213 9.37e-79 232
Msa1109810 MtrunA17_Chr7g0244361 81.679 131 24 0 6 136 113 243 1.82e-77 229
Msa1109810 MtrunA17_Chr7g0244321 82.171 129 23 0 6 134 84 212 7.82e-77 226
Msa1109810 MtrunA17_Chr7g0244331 79.389 131 27 0 4 134 82 212 1.07e-75 224
Msa1109810 MtrunA17_Chr7g0244111 80.992 121 23 0 11 131 1 121 3.88e-74 216
Msa1109810 MtrunA17_Chr4g0013291 65.079 126 44 0 6 131 72 197 6.25e-58 178
Msa1109810 MtrunA17_Chr1g0150081 58.772 114 43 2 12 125 75 184 4.66e-39 130
Msa1109810 MtrunA17_Chr1g0150091 43.382 136 54 5 6 125 2 130 3.27e-22 85.5
Msa1109810 MtrunA17_Chr1g0150101 54.430 79 32 2 47 125 4 78 2.33e-20 79.0
Msa1109810 MtrunA17_Chr2g0333101 30.833 120 77 4 15 132 90 205 9.49e-18 75.9
Msa1109810 MtrunA17_Chr5g0432221 37.037 108 63 3 20 124 105 210 1.15e-16 73.2
Msa1109810 MtrunA17_Chr4g0003271 27.119 118 82 4 15 131 79 193 6.14e-16 70.9
Msa1109810 MtrunA17_Chr2g0333111 28.571 119 79 4 15 131 91 205 1.39e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1109810 AT5G01750.2 62.903 124 46 0 6 129 88 211 7.54e-59 181
Msa1109810 AT3G11740.1 64.957 117 40 1 14 129 72 188 1.55e-53 167
Msa1109810 AT1G33840.1 55.645 124 55 0 6 129 88 211 6.54e-46 149
Msa1109810 AT2G14560.1 54.032 124 57 0 6 129 68 191 9.86e-46 147
Msa1109810 AT2G14560.3 54.032 124 57 0 6 129 128 251 1.17e-44 147
Msa1109810 AT5G01750.1 60.000 85 34 0 6 90 88 172 1.96e-35 120
Msa1109810 AT3G16900.1 42.400 125 60 3 6 129 67 180 1.76e-32 113
Msa1109810 AT2G14560.2 51.163 86 42 0 6 91 68 153 3.76e-30 107
Msa1109810 AT2G14560.4 51.163 86 42 0 6 91 128 213 4.25e-29 106
Msa1109810 AT1G33840.2 52.326 86 41 0 6 91 88 173 7.07e-28 101
Msa1109810 AT3G56180.2 34.328 134 79 3 4 134 77 204 2.22e-19 80.5
Msa1109810 AT3G56180.1 33.582 134 78 4 4 134 77 202 2.97e-17 74.7
Msa1109810 AT3G15810.1 29.060 117 79 2 15 131 92 204 2.01e-16 72.8
Msa1109810 AT2G30270.1 33.962 106 64 3 20 124 78 178 1.00e-15 70.1
Msa1109810 AT3G10986.1 32.283 127 77 3 13 134 73 195 2.32e-15 69.7
Msa1109810 AT1G80120.1 30.702 114 73 3 20 131 84 193 7.53e-14 65.9

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGTCTTAAATTGCATT+AGG 0.261327 7_3:-54254882 None:intergenic
TTTGGTCTTAAATTGCATTA+GGG 0.330409 7_3:-54254881 None:intergenic
AGCAAGAAACACATCTAATT+TGG 0.342809 7_3:-54254899 None:intergenic
GTGGCATTAATCGTGATTCT+TGG 0.373611 7_3:+54255192 Msa1109810:CDS
GATAAACTGTGACCATAAAA+TGG 0.388429 7_3:-54255146 None:intergenic
TATAAAAGCGTAATCAATGT+TGG 0.462661 7_3:-54255169 None:intergenic
ATAAAAGCGTAATCAATGTT+GGG 0.479628 7_3:-54255168 None:intergenic
ATCTAATAACATTGTTGCAC+AGG 0.491705 7_3:+54255002 Msa1109810:CDS
CGATCTTGCACTGTTTATGC+TGG 0.521359 7_3:+54254976 Msa1109810:CDS
ACTTTAAAGTCAGAGGCAGT+TGG 0.523990 7_3:+54254947 Msa1109810:CDS
ATTGACTATGCATGATCGGT+GGG 0.561743 7_3:+54254798 Msa1109810:CDS
GATCGGTGGGAAGCGTATAG+AGG 0.562084 7_3:+54254811 Msa1109810:CDS
AGGTCAAAGCACAAATGCTA+AGG 0.563005 7_3:+54254831 Msa1109810:CDS
GTTTGTGACTTTAAAGTCAG+AGG 0.577508 7_3:+54254940 Msa1109810:CDS
AGATATTGACTATGCATGAT+CGG 0.578461 7_3:+54254794 Msa1109810:CDS
TATTGACTATGCATGATCGG+TGG 0.603063 7_3:+54254797 Msa1109810:CDS
ATGCAAAGAATAGTAACTAG+AGG 0.634925 7_3:+54255229 Msa1109810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATCACAAATTAATTTTTG+TGG + chr7_3:54255058-54255077 Msa1109810:intron 15.0%
!!! TTTGGTCTTAAATTGCATTA+GGG - chr7_3:54254884-54254903 None:intergenic 25.0%
!!! ATTTGGTCTTAAATTGCATT+AGG - chr7_3:54254885-54254904 None:intergenic 25.0%
!! AATTGGTAAAGACCATTTTA+TGG + chr7_3:54255134-54255153 Msa1109810:CDS 25.0%
!! ATAAAAGCGTAATCAATGTT+GGG - chr7_3:54255171-54255190 None:intergenic 25.0%
!! TATAAAAGCGTAATCAATGT+TGG - chr7_3:54255172-54255191 None:intergenic 25.0%
!! ATTTTCAATTCGTGTGTAAT+AGG + chr7_3:54255286-54255305 Msa1109810:three_prime_UTR 25.0%
! AGATATTGACTATGCATGAT+CGG + chr7_3:54254794-54254813 Msa1109810:CDS 30.0%
AGCAAGAAACACATCTAATT+TGG - chr7_3:54254902-54254921 None:intergenic 30.0%
ATCTAATAACATTGTTGCAC+AGG + chr7_3:54255002-54255021 Msa1109810:CDS 30.0%
GATAAACTGTGACCATAAAA+TGG - chr7_3:54255149-54255168 None:intergenic 30.0%
!! CATTGATTACGCTTTTATAG+TGG + chr7_3:54255173-54255192 Msa1109810:CDS 30.0%
ATGCAAAGAATAGTAACTAG+AGG + chr7_3:54255229-54255248 Msa1109810:CDS 30.0%
!! GTTTGTGACTTTAAAGTCAG+AGG + chr7_3:54254940-54254959 Msa1109810:CDS 35.0%
!! ACGGTTACGAGCATTTTAAT+TGG + chr7_3:54255117-54255136 Msa1109810:CDS 35.0%
! TATTGACTATGCATGATCGG+TGG + chr7_3:54254797-54254816 Msa1109810:CDS 40.0%
ATTGACTATGCATGATCGGT+GGG + chr7_3:54254798-54254817 Msa1109810:CDS 40.0%
AGGTCAAAGCACAAATGCTA+AGG + chr7_3:54254831-54254850 Msa1109810:CDS 40.0%
!! ACTTTAAAGTCAGAGGCAGT+TGG + chr7_3:54254947-54254966 Msa1109810:CDS 40.0%
GTGGCATTAATCGTGATTCT+TGG + chr7_3:54255192-54255211 Msa1109810:CDS 40.0%
CGATCTTGCACTGTTTATGC+TGG + chr7_3:54254976-54254995 Msa1109810:CDS 45.0%
! GCAGATGCACAAAAAGCACA+CGG + chr7_3:54255098-54255117 Msa1109810:intron 45.0%
! GATCGGTGGGAAGCGTATAG+AGG + chr7_3:54254811-54254830 Msa1109810:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 54254760 54255324 54254760 ID=Msa1109810;Name=Msa1109810
chr7_3 mRNA 54254760 54255324 54254760 ID=Msa1109810-mRNA-1;Parent=Msa1109810;Name=Msa1109810-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|1|0.5|1|1|1|2|76|136
chr7_3 exon 54254760 54255023 54254760 ID=Msa1109810-mRNA-1:exon:11384;Parent=Msa1109810-mRNA-1
chr7_3 exon 54255102 54255324 54255102 ID=Msa1109810-mRNA-1:exon:11385;Parent=Msa1109810-mRNA-1
chr7_3 CDS 54254760 54255023 54254760 ID=Msa1109810-mRNA-1:cds;Parent=Msa1109810-mRNA-1
chr7_3 CDS 54255102 54255248 54255102 ID=Msa1109810-mRNA-1:cds;Parent=Msa1109810-mRNA-1
chr7_3 three_prime_UTR 54255249 54255324 54255249 ID=Msa1109810-mRNA-1:three_prime_utr;Parent=Msa1109810-mRNA-1
Gene Sequence

>Msa1109810

ATGTTTCATTTTTTTGTTGTACTACTATTTGTACAGATATTGACTATGCATGATCGGTGGGAAGCGTATAGAGGTCAAAGCACAAATGCTAAGGATCTAATATTTACATTGAAACGATCATCCCTAATGCAATTTAAGACCAAATTAGATGTGTTTCTTGCTAGTAACACAAAAGAAGATGTTTGTGACTTTAAAGTCAGAGGCAGTTGGTTTGAACGATCTTGCACTGTTTATGCTGGTGAATCTAATAACATTGTTGCACAGATGCACAAAAAGCACACGGTTACGAGCATTTTAATTGGTAAAGACCATTTTATGGTCACAGTTTATCCCAACATTGATTACGCTTTTATAGTGGCATTAATCGTGATTCTTGGCGAGATTAACGATGATGCAAAGAATAGTAACTAG

Protein sequence

>Msa1109810

MFHFFVVLLFVQILTMHDRWEAYRGQSTNAKDLIFTLKRSSLMQFKTKLDVFLASNTKEDVCDFKVRGSWFERSCTVYAGESNNIVAQMHKKHTVTSILIGKDHFMVTVYPNIDYAFIVALIVILGEINDDAKNSN*