Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1082360 | XP_003625963.1 | 98.182 | 220 | 3 | 1 | 1 | 220 | 1 | 219 | 5.88e-155 | 438 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1082360 | G7KST0 | 98.182 | 220 | 3 | 1 | 1 | 220 | 1 | 219 | 2.81e-155 | 438 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001830 | Msa1082360 | 0.842345 | 2.737064e-58 | -8.615850e-47 |
| Msa0016510 | Msa1082360 | 0.809904 | 1.502200e-50 | -8.615850e-47 |
| Msa0043850 | Msa1082360 | 0.858844 | 6.231126e-63 | -8.615850e-47 |
| Msa0077370 | Msa1082360 | 0.855027 | 8.306024e-62 | -8.615850e-47 |
| Msa0077380 | Msa1082360 | 0.803837 | 2.880482e-49 | -8.615850e-47 |
| Msa0082420 | Msa1082360 | 0.825653 | 4.168978e-54 | -8.615850e-47 |
| Msa0090510 | Msa1082360 | 0.854797 | 9.689725e-62 | -8.615850e-47 |
| Msa0117520 | Msa1082360 | 0.817052 | 4.027108e-52 | -8.615850e-47 |
| Msa0641130 | Msa1082360 | 0.805412 | 1.351697e-49 | -8.615850e-47 |
| Msa0654900 | Msa1082360 | 0.830078 | 3.596599e-55 | -8.615850e-47 |
| Msa0657120 | Msa1082360 | 0.835170 | 1.961392e-56 | -8.615850e-47 |
| Msa0697680 | Msa1082360 | 0.800472 | 1.418476e-48 | -8.615850e-47 |
| Msa0698450 | Msa1082360 | 0.826249 | 3.008358e-54 | -8.615850e-47 |
| Msa0698460 | Msa1082360 | 0.842203 | 2.983850e-58 | -8.615850e-47 |
| Msa0701100 | Msa1082360 | 0.829103 | 6.208538e-55 | -8.615850e-47 |
| Msa0703810 | Msa1082360 | 0.810175 | 1.313586e-50 | -8.615850e-47 |
| Msa0705990 | Msa1082360 | 0.803389 | 3.567710e-49 | -8.615850e-47 |
| Msa0709120 | Msa1082360 | 0.821447 | 4.018691e-53 | -8.615850e-47 |
| Msa0764920 | Msa1082360 | 0.819114 | 1.376604e-52 | -8.615850e-47 |
| Msa0796580 | Msa1082360 | -0.802969 | 4.359912e-49 | -8.615850e-47 |
| Msa0812950 | Msa1082360 | 0.810070 | 1.383669e-50 | -8.615850e-47 |
| Msa0823540 | Msa1082360 | 0.800640 | 1.310766e-48 | -8.615850e-47 |
| Msa0136630 | Msa1082360 | 0.820648 | 6.139152e-53 | -8.615850e-47 |
| Msa0137980 | Msa1082360 | 0.849674 | 2.779310e-60 | -8.615850e-47 |
| Msa0138900 | Msa1082360 | 0.816978 | 4.183959e-52 | -8.615850e-47 |
| Msa0139670 | Msa1082360 | 0.820886 | 5.411007e-53 | -8.615850e-47 |
| Msa0160960 | Msa1082360 | 0.819994 | 8.671249e-53 | -8.615850e-47 |
| Msa0183910 | Msa1082360 | 0.851226 | 1.019018e-60 | -8.615850e-47 |
| Msa0196670 | Msa1082360 | 0.826606 | 2.473160e-54 | -8.615850e-47 |
| Msa0213430 | Msa1082360 | 0.802213 | 6.242379e-49 | -8.615850e-47 |
| Msa0213540 | Msa1082360 | 0.816831 | 4.514415e-52 | -8.615850e-47 |
| Msa0233370 | Msa1082360 | 0.823666 | 1.224772e-53 | -8.615850e-47 |
| Msa0364790 | Msa1082360 | 0.831289 | 1.815857e-55 | -8.615850e-47 |
| Msa0380600 | Msa1082360 | 0.809285 | 2.041122e-50 | -8.615850e-47 |
| Msa0386940 | Msa1082360 | 0.815683 | 8.155398e-52 | -8.615850e-47 |
| Msa0388730 | Msa1082360 | 0.804023 | 2.635730e-49 | -8.615850e-47 |
| Msa0403490 | Msa1082360 | 0.801847 | 7.420731e-49 | -8.615850e-47 |
| Msa0403770 | Msa1082360 | 0.800977 | 1.118801e-48 | -8.615850e-47 |
| Msa0412260 | Msa1082360 | 0.808470 | 3.048898e-50 | -8.615850e-47 |
| Msa0430240 | Msa1082360 | 0.805231 | 1.475189e-49 | -8.615850e-47 |
| Msa0431900 | Msa1082360 | 0.814837 | 1.256837e-51 | -8.615850e-47 |
| Msa0456770 | Msa1082360 | 0.828129 | 1.067113e-54 | -8.615850e-47 |
| Msa0982090 | Msa1082360 | -0.827850 | 1.245895e-54 | -8.615850e-47 |
| Msa0994370 | Msa1082360 | 0.825338 | 4.950274e-54 | -8.615850e-47 |
| Msa1013070 | Msa1082360 | 0.844382 | 7.828467e-59 | -8.615850e-47 |
| Msa1023310 | Msa1082360 | 0.801889 | 7.275178e-49 | -8.615850e-47 |
| Msa1029520 | Msa1082360 | 0.825877 | 3.688371e-54 | -8.615850e-47 |
| Msa1044510 | Msa1082360 | 0.820270 | 7.495464e-53 | -8.615850e-47 |
| Msa1062040 | Msa1082360 | 0.862232 | 5.863078e-64 | -8.615850e-47 |
| Msa1063360 | Msa1082360 | 0.807752 | 4.336123e-50 | -8.615850e-47 |
| Msa1070520 | Msa1082360 | 0.802562 | 5.290134e-49 | -8.615850e-47 |
| Msa1070540 | Msa1082360 | 0.832585 | 8.690863e-56 | -8.615850e-47 |
| Msa1082360 | Msa1089460 | 0.804741 | 1.867597e-49 | -8.615850e-47 |
| Msa1082360 | Msa1094280 | 0.816280 | 5.999031e-52 | -8.615850e-47 |
| Msa1082360 | Msa1101240 | 0.826327 | 2.882851e-54 | -8.615850e-47 |
| Msa1082360 | Msa1107200 | 0.838616 | 2.587455e-57 | -8.615850e-47 |
| Msa1082360 | Msa1108710 | 0.805454 | 1.324341e-49 | -8.615850e-47 |
| Msa1082360 | Msa1109810 | 0.862717 | 4.157449e-64 | -8.615850e-47 |
| Msa1082360 | Msa1127260 | 0.866131 | 3.566301e-65 | -8.615850e-47 |
| Msa1082360 | Msa1150780 | 0.829736 | 4.356419e-55 | -8.615850e-47 |
| Msa1082360 | Msa1183140 | 0.855579 | 5.738041e-62 | -8.615850e-47 |
| Msa1082360 | Msa1205820 | 0.802818 | 4.684001e-49 | -8.615850e-47 |
| Msa1082360 | Msa1206270 | 0.832599 | 8.624144e-56 | -8.615850e-47 |
| Msa1082360 | Msa1229480 | 0.873315 | 1.616706e-67 | -8.615850e-47 |
| Msa1082360 | Msa1234620 | 0.800851 | 1.187090e-48 | -8.615850e-47 |
| Msa1082360 | Msa1238680 | 0.825868 | 3.706636e-54 | -8.615850e-47 |
| Msa1082360 | Msa1256810 | 0.822405 | 2.410441e-53 | -8.615850e-47 |
| Msa1082360 | Msa1272010 | 0.836718 | 7.939998e-57 | -8.615850e-47 |
| Msa1082360 | Msa1280170 | 0.807655 | 4.545443e-50 | -8.615850e-47 |
| Msa1082360 | Msa1298010 | 0.809825 | 1.562730e-50 | -8.615850e-47 |
| Msa1082360 | Msa1307160 | 0.816595 | 5.101189e-52 | -8.615850e-47 |
| Msa1082360 | Msa1307180 | 0.814263 | 1.684248e-51 | -8.615850e-47 |
| Msa1082360 | Msa1309760 | 0.835657 | 1.476761e-56 | -8.615850e-47 |
| Msa1082360 | Msa1309870 | 0.818574 | 1.825758e-52 | -8.615850e-47 |
| Msa1082360 | Msa1316360 | 0.825640 | 4.197745e-54 | -8.615850e-47 |
| Msa1082360 | Msa1368610 | 0.813321 | 2.715172e-51 | -8.615850e-47 |
| Msa1082360 | Msa1382020 | 0.825446 | 4.667126e-54 | -8.615850e-47 |
| Msa1082360 | Msa1382380 | -0.829136 | 6.094364e-55 | -8.615850e-47 |
| Msa1082360 | Msa1389640 | 0.837736 | 4.359337e-57 | -8.615850e-47 |
| Msa1082360 | Msa1407760 | 0.830010 | 3.735361e-55 | -8.615850e-47 |
| Msa1082360 | Msa1437730 | 0.804978 | 1.666248e-49 | -8.615850e-47 |
| Msa1082360 | Msa1440190 | 0.800297 | 1.539857e-48 | -8.615850e-47 |
| Msa1082360 | Msa1449750 | 0.800088 | 1.698316e-48 | -8.615850e-47 |
| Msa0256390 | Msa1082360 | 0.826575 | 2.516559e-54 | -8.615850e-47 |
| Msa0276100 | Msa1082360 | 0.824182 | 9.273367e-54 | -8.615850e-47 |
| Msa0276430 | Msa1082360 | 0.815579 | 8.599105e-52 | -8.615850e-47 |
| Msa0317960 | Msa1082360 | 0.806028 | 1.003371e-49 | -8.615850e-47 |
| Msa0331020 | Msa1082360 | 0.835569 | 1.554699e-56 | -8.615850e-47 |
| Msa0484630 | Msa1082360 | 0.813653 | 2.295306e-51 | -8.615850e-47 |
| Msa0518170 | Msa1082360 | 0.803796 | 2.937777e-49 | -8.615850e-47 |
| Msa0520770 | Msa1082360 | 0.811126 | 8.183108e-51 | -8.615850e-47 |
| Msa0528990 | Msa1082360 | 0.819646 | 1.041239e-52 | -8.615850e-47 |
| Msa0529000 | Msa1082360 | 0.859020 | 5.518590e-63 | -8.615850e-47 |
| Msa0534870 | Msa1082360 | 0.808817 | 2.570936e-50 | -8.615850e-47 |
| Msa0545050 | Msa1082360 | 0.816841 | 4.490775e-52 | -8.615850e-47 |
| Msa0561360 | Msa1082360 | 0.801063 | 1.074403e-48 | -8.615850e-47 |
| Msa0574930 | Msa1082360 | 0.833159 | 6.257764e-56 | -8.615850e-47 |
| Msa0600590 | Msa1082360 | 0.821319 | 4.301622e-53 | -8.615850e-47 |
| Msa0844610 | Msa1082360 | 0.812559 | 3.989516e-51 | -8.615850e-47 |
| Msa0881530 | Msa1082360 | -0.809953 | 1.466064e-50 | -8.615850e-47 |
| Msa0930740 | Msa1082360 | 0.814047 | 1.879140e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1082360 | MtrunA17_Chr7g0269921 | 98.182 | 220 | 3 | 1 | 1 | 220 | 1 | 219 | 5.41e-159 | 438 |
| Msa1082360 | MtrunA17_Chr1g0194751 | 59.912 | 227 | 74 | 4 | 1 | 216 | 1 | 221 | 3.31e-78 | 234 |
| Msa1082360 | MtrunA17_Chr1g0208551 | 37.089 | 213 | 120 | 5 | 17 | 219 | 56 | 264 | 3.81e-46 | 154 |
| Msa1082360 | MtrunA17_Chr7g0251421 | 34.466 | 206 | 122 | 6 | 19 | 219 | 15 | 212 | 6.08e-37 | 128 |
| Msa1082360 | MtrunA17_Chr8g0361661 | 38.587 | 184 | 101 | 3 | 9 | 183 | 56 | 236 | 5.34e-35 | 125 |
| Msa1082360 | MtrunA17_Chr5g0417141 | 38.378 | 185 | 100 | 5 | 20 | 194 | 70 | 250 | 1.40e-30 | 114 |
| Msa1082360 | MtrunA17_Chr7g0217321 | 35.577 | 104 | 59 | 3 | 1 | 102 | 16 | 113 | 1.50e-16 | 75.5 |
| Msa1082360 | MtrunA17_Chr3g0099671 | 51.613 | 62 | 6 | 1 | 32 | 93 | 3 | 40 | 1.31e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1082360 | AT1G02610.1 | 50.505 | 198 | 89 | 5 | 1 | 194 | 1 | 193 | 3.53e-60 | 188 |
| Msa1082360 | AT1G02610.2 | 50.505 | 198 | 89 | 5 | 1 | 194 | 1 | 193 | 4.00e-60 | 187 |
| Msa1082360 | AT4G02075.1 | 49.485 | 194 | 87 | 5 | 1 | 192 | 1 | 185 | 2.23e-54 | 173 |
| Msa1082360 | AT4G02075.3 | 48.969 | 194 | 88 | 5 | 1 | 192 | 1 | 185 | 3.83e-54 | 172 |
| Msa1082360 | AT2G01275.3 | 43.750 | 176 | 85 | 6 | 16 | 185 | 81 | 248 | 3.35e-44 | 149 |
| Msa1082360 | AT2G01275.5 | 43.750 | 176 | 85 | 6 | 16 | 185 | 81 | 248 | 3.35e-44 | 149 |
| Msa1082360 | AT2G01275.2 | 43.750 | 176 | 85 | 6 | 16 | 185 | 55 | 222 | 3.49e-44 | 149 |
| Msa1082360 | AT2G01275.4 | 43.750 | 176 | 85 | 6 | 16 | 185 | 55 | 222 | 3.49e-44 | 149 |
| Msa1082360 | AT2G01275.1 | 43.750 | 176 | 85 | 6 | 16 | 185 | 55 | 222 | 3.49e-44 | 149 |
| Msa1082360 | AT4G02075.2 | 49.112 | 169 | 75 | 5 | 1 | 167 | 1 | 160 | 9.47e-44 | 145 |
| Msa1082360 | AT2G02960.3 | 38.342 | 193 | 107 | 4 | 20 | 203 | 43 | 232 | 2.96e-33 | 120 |
| Msa1082360 | AT2G02960.2 | 38.342 | 193 | 107 | 4 | 20 | 203 | 43 | 232 | 2.96e-33 | 120 |
| Msa1082360 | AT2G02960.6 | 38.342 | 193 | 107 | 4 | 20 | 203 | 43 | 232 | 2.96e-33 | 120 |
| Msa1082360 | AT2G02960.4 | 38.342 | 193 | 107 | 4 | 20 | 203 | 43 | 232 | 2.96e-33 | 120 |
| Msa1082360 | AT2G02960.1 | 38.342 | 193 | 107 | 4 | 20 | 203 | 43 | 232 | 2.96e-33 | 120 |
| Msa1082360 | AT5G38070.1 | 36.612 | 183 | 93 | 5 | 15 | 186 | 49 | 219 | 1.75e-32 | 118 |
| Msa1082360 | AT3G47550.2 | 38.983 | 177 | 93 | 4 | 20 | 186 | 69 | 240 | 7.20e-30 | 111 |
| Msa1082360 | AT3G47550.3 | 39.459 | 185 | 96 | 5 | 20 | 194 | 69 | 247 | 7.31e-30 | 112 |
| Msa1082360 | AT3G47550.6 | 39.459 | 185 | 96 | 5 | 20 | 194 | 69 | 247 | 7.31e-30 | 112 |
| Msa1082360 | AT5G62460.1 | 39.674 | 184 | 99 | 4 | 20 | 194 | 78 | 258 | 3.50e-27 | 105 |
| Msa1082360 | AT5G62460.2 | 39.674 | 184 | 99 | 4 | 20 | 194 | 78 | 258 | 3.50e-27 | 105 |
| Msa1082360 | AT5G62460.3 | 39.674 | 184 | 99 | 4 | 20 | 194 | 91 | 271 | 3.58e-27 | 106 |
| Msa1082360 | AT1G14260.2 | 34.359 | 195 | 116 | 4 | 18 | 203 | 55 | 246 | 4.40e-27 | 104 |
| Msa1082360 | AT1G14260.1 | 34.359 | 195 | 116 | 4 | 18 | 203 | 55 | 246 | 4.40e-27 | 104 |
| Msa1082360 | AT1G14260.3 | 34.359 | 195 | 116 | 4 | 18 | 203 | 55 | 246 | 4.40e-27 | 104 |
| Msa1082360 | AT2G02960.5 | 43.066 | 137 | 68 | 3 | 20 | 149 | 43 | 176 | 5.49e-26 | 102 |
| Msa1082360 | AT3G47550.1 | 42.623 | 122 | 56 | 3 | 20 | 132 | 69 | 185 | 3.90e-23 | 93.6 |
| Msa1082360 | AT1G02610.3 | 37.984 | 129 | 73 | 4 | 70 | 194 | 2 | 127 | 6.79e-20 | 83.2 |
Find 50 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTACTAATCGATGATTTA+AGG | 0.158832 | 7_2:+82463286 | Msa1082360:CDS |
| AGAAAATTTAATACATTCAT+AGG | 0.223917 | 7_2:+82465726 | Msa1082360:three_prime_UTR |
| GATGATTTAAGGTTACTTTC+TGG | 0.231213 | 7_2:+82463297 | Msa1082360:CDS |
| TCATTTATCTCTGATTTCTT+TGG | 0.231558 | 7_2:-82464192 | Msa1082370:three_prime_UTR |
| CTTACGGTAAGAAGTGTAAA+TGG | 0.234459 | 7_2:-82465389 | Msa1082370:intron |
| TACATTCATAGAGTATTTAA+TGG | 0.256613 | 7_2:+82465682 | Msa1082360:exon |
| GAAGCACCTTGTTCTTGTTC+TGG | 0.299149 | 7_2:+82463375 | Msa1082360:CDS |
| CGCGTTTCACTGTCGATCAT+TGG | 0.320595 | 7_2:+82464590 | Msa1082360:CDS |
| ACAAGAACAAGGTGCTTCTA+AGG | 0.336965 | 7_2:-82463370 | Msa1082370:three_prime_UTR |
| ACAAGGTGCTTCTAAGGCTT+TGG | 0.357018 | 7_2:-82463364 | Msa1082370:three_prime_UTR |
| GTTGTAGGTCATTGTTCTAA+AGG | 0.358971 | 7_2:+82465512 | Msa1082360:intron |
| TTTGAAAACAAAGTGATCAT+TGG | 0.367671 | 7_2:+82463231 | Msa1082360:five_prime_UTR |
| AGCTTTCAAACTCTTCTTCA+TGG | 0.379338 | 7_2:-82463341 | Msa1082370:three_prime_UTR |
| TTCTTGTTCTGGAACTGTTA+AGG | 0.379888 | 7_2:+82463386 | Msa1082360:CDS |
| TAGTGGTAGAGCTGCATTCC+GGG | 0.387977 | 7_2:-82464560 | Msa1082370:three_prime_UTR |
| TTAGTGGTAGAGCTGCATTC+CGG | 0.388354 | 7_2:-82464561 | Msa1082370:three_prime_UTR |
| TTGAAAACAAAGTGATCATT+GGG | 0.405202 | 7_2:+82463232 | Msa1082360:five_prime_UTR |
| TTTATCTCTGATTTCTTTGG+AGG | 0.412787 | 7_2:-82464189 | Msa1082370:three_prime_UTR |
| GTTACTAGTAAGACATTGTT+TGG | 0.431240 | 7_2:+82465337 | Msa1082360:CDS |
| ATCTCTGATTTCTTTGGAGG+AGG | 0.446591 | 7_2:-82464186 | Msa1082370:three_prime_UTR |
| AACGCGGTAAAGTACCTTAT+GGG | 0.451704 | 7_2:-82464225 | Msa1082370:intron |
| TAATCTTCGGTTCCACTTGT+GGG | 0.462880 | 7_2:-82465365 | Msa1082370:three_prime_UTR |
| ATGATGAAGCAATGCCCATA+AGG | 0.464358 | 7_2:+82464211 | Msa1082360:CDS |
| GTGAAACGCGGTTCTGTTAG+TGG | 0.469197 | 7_2:-82464577 | Msa1082370:three_prime_UTR |
| GAGCCATTCTGAATAGCATT+AGG | 0.470162 | 7_2:+82465580 | Msa1082360:CDS |
| GTAATCTTCGGTTCCACTTG+TGG | 0.490070 | 7_2:-82465366 | Msa1082370:three_prime_UTR |
| CAGAATGGCTCCGATTGTCT+TGG | 0.492886 | 7_2:-82465568 | Msa1082370:three_prime_UTR |
| TACATAGTTACCAAGACAAT+CGG | 0.492892 | 7_2:+82465558 | Msa1082360:CDS |
| AATAGCATTAGGCGCTATCA+AGG | 0.494672 | 7_2:+82465591 | Msa1082360:CDS |
| GGAGGAGGAGCTGTATATCC+AGG | 0.497108 | 7_2:-82464171 | Msa1082370:three_prime_UTR |
| TGTCTTGGTAACTATGTACA+TGG | 0.500550 | 7_2:-82465553 | Msa1082370:three_prime_UTR |
| AAGTGTAAATGGGTAATCTT+CGG | 0.501901 | 7_2:-82465378 | Msa1082370:three_prime_UTR |
| CAAAGATGGTGTAACGAGAA+AGG | 0.507130 | 7_2:+82463568 | Msa1082360:CDS |
| TTACGGTAAGAAGTGTAAAT+GGG | 0.521582 | 7_2:-82465388 | Msa1082370:intron |
| GAAAATTTAATACATTCATA+GGG | 0.529541 | 7_2:+82465727 | Msa1082360:three_prime_UTR |
| TAATGGCAGAAATGAAGAGC+AGG | 0.545128 | 7_2:+82465699 | Msa1082360:three_prime_UTR |
| ATAGAGATTGCATACAAAGA+TGG | 0.547272 | 7_2:+82463554 | Msa1082360:CDS |
| AACATGTGAAATATGTCTCC+AGG | 0.548388 | 7_2:+82463597 | Msa1082360:CDS |
| GTCATTGTTCTAAAGGCTTG+CGG | 0.555397 | 7_2:+82465519 | Msa1082360:CDS |
| GCGCCTAATGCTATTCAGAA+TGG | 0.556703 | 7_2:-82465583 | Msa1082370:three_prime_UTR |
| ACATCACTCTCTGATGAAGA+TGG | 0.559466 | 7_2:+82465627 | Msa1082360:CDS |
| AGATGGCAGAAATGAAGAGC+AGG | 0.567472 | 7_2:+82465644 | Msa1082360:CDS |
| TGTAATAGAAAGTGATTACC+CGG | 0.600485 | 7_2:+82464542 | Msa1082360:CDS |
| TGTTTGGTAGTGCCCACAAG+TGG | 0.604389 | 7_2:+82465353 | Msa1082360:CDS |
| AAAAGAGAAAGTGAGGTGGA+AGG | 0.629605 | 7_2:+82464516 | Msa1082360:CDS |
| CGCGAAAAGAGAAAGTGAGG+TGG | 0.646596 | 7_2:+82464512 | Msa1082360:CDS |
| AAACGCGAAAAGAGAAAGTG+AGG | 0.648637 | 7_2:+82464509 | Msa1082360:CDS |
| GTCTTGGTAACTATGTACAT+GGG | 0.665639 | 7_2:-82465552 | Msa1082370:three_prime_UTR |
| CAATGATCGACAGTGAAACG+CGG | 0.703113 | 7_2:-82464589 | Msa1082370:three_prime_UTR |
| ACAGTTCCAGAACAAGAACA+AGG | 0.719305 | 7_2:-82463381 | Msa1082370:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATAACACATTTAAT+TGG | + | chr7_2:82464411-82464430 | Msa1082360:intron | 10.0% |
| !! | TTTCATTCAAAATTAAAATA+CGG | - | chr7_2:82465428-82465447 | Msa1082370:intron | 10.0% |
| !! | AATAGAAAGAAAAAATAAAG+AGG | + | chr7_2:82463746-82463765 | Msa1082360:intron | 15.0% |
| !! | ATAGAAAGAAAAAATAAAGA+GGG | + | chr7_2:82463747-82463766 | Msa1082360:intron | 15.0% |
| !! | ATATAAGTCAAAATTAGATT+GGG | - | chr7_2:82463847-82463866 | Msa1082370:intron | 15.0% |
| !! | TATATAAGTCAAAATTAGAT+TGG | - | chr7_2:82463848-82463867 | Msa1082370:intron | 15.0% |
| !! | CTACAAATTATAATATCAAA+TGG | - | chr7_2:82464019-82464038 | Msa1082370:intron | 15.0% |
| !!! | CATTTGATATTATAATTTGT+AGG | + | chr7_2:82464017-82464036 | Msa1082360:intron | 15.0% |
| !!! | TATTATAATTTGTAGGAATT+AGG | + | chr7_2:82464024-82464043 | Msa1082360:intron | 15.0% |
| !!! | AAATTTGATAACACATTTTA+AGG | + | chr7_2:82464469-82464488 | Msa1082360:intron | 15.0% |
| !!! | AATTTGATAACACATTTTAA+GGG | + | chr7_2:82464470-82464489 | Msa1082360:intron | 15.0% |
| !!! | AAAAACTGTAAGTTATTTTT+CGG | - | chr7_2:82465297-82465316 | Msa1082370:intron | 15.0% |
| !!! | GTATTTTAATTTTGAATGAA+AGG | + | chr7_2:82465427-82465446 | Msa1082360:intron | 15.0% |
| !! | AGAAAATTTAATACATTCAT+AGG | + | chr7_2:82465671-82465690 | Msa1082360:CDS | 15.0% |
| !! | GAAAATTTAATACATTCATA+GGG | + | chr7_2:82465672-82465691 | Msa1082360:CDS | 15.0% |
| !! | TGCATGATAAATATAAAAGA+TGG | + | chr7_2:82464097-82464116 | Msa1082360:intron | 20.0% |
| !! | GCATGATAAATATAAAAGAT+GGG | + | chr7_2:82464098-82464117 | Msa1082360:intron | 20.0% |
| !!! | ATTTGATAACACATTTTAAG+GGG | + | chr7_2:82464471-82464490 | Msa1082360:intron | 20.0% |
| !! | AAATAAAGTTGCATGAATTT+AGG | + | chr7_2:82464734-82464753 | Msa1082360:intron | 20.0% |
| !! | AATAAAGTTGCATGAATTTA+GGG | + | chr7_2:82464735-82464754 | Msa1082360:intron | 20.0% |
| !!! | TTTGTTATATAAACTCTTCT+AGG | - | chr7_2:82464835-82464854 | Msa1082370:intron | 20.0% |
| !!! | TACATTCATAGAGTATTTAA+TGG | + | chr7_2:82465682-82465701 | Msa1082360:exon | 20.0% |
| ! | TTTGAAAACAAAGTGATCAT+TGG | + | chr7_2:82463231-82463250 | Msa1082360:five_prime_UTR | 25.0% |
| ! | TTGAAAACAAAGTGATCATT+GGG | + | chr7_2:82463232-82463251 | Msa1082360:five_prime_UTR | 25.0% |
| ! | TGTTACTAATCGATGATTTA+AGG | + | chr7_2:82463286-82463305 | Msa1082360:CDS | 25.0% |
| ! | AATTATGTTGTGACGTAAAT+TGG | + | chr7_2:82463813-82463832 | Msa1082360:intron | 25.0% |
| ! | TTAAATTTCTCAGCTAATCA+TGG | + | chr7_2:82463971-82463990 | Msa1082360:intron | 25.0% |
| ! | GAAATGAAGATAGAACTAAA+GGG | - | chr7_2:82463997-82464016 | Msa1082370:intron | 25.0% |
| ! | TTATGTTCATTGTATGCATA+AGG | - | chr7_2:82464060-82464079 | Msa1082370:intron | 25.0% |
| ! | TCATTTATCTCTGATTTCTT+TGG | - | chr7_2:82464195-82464214 | Msa1082370:three_prime_UTR | 25.0% |
| !!! | CTTTTTGATTGAATTAAACG+CGG | - | chr7_2:82464244-82464263 | Msa1082370:intron | 25.0% |
| !!! | TTTGATAACACATTTTAAGG+GGG | + | chr7_2:82464472-82464491 | Msa1082360:intron | 25.0% |
| !! | TAATGCTATGCTATTTGATT+AGG | - | chr7_2:82464674-82464693 | Msa1082370:intron | 25.0% |
| !! | AGAAAACAATACACGTTTTT+GGG | - | chr7_2:82464920-82464939 | Msa1082370:intron | 25.0% |
| !! | TAGAAAACAATACACGTTTT+TGG | - | chr7_2:82464921-82464940 | Msa1082370:intron | 25.0% |
| !! | ACGATTACTTTCAAACTTTT+AGG | + | chr7_2:82465035-82465054 | Msa1082360:intron | 25.0% |
| !! | TTTAATTTAACCTGCAGTTA+GGG | + | chr7_2:82465193-82465212 | Msa1082360:intron | 25.0% |
| ! | TTCATAAAATGCCATTAGTA+CGG | - | chr7_2:82465484-82465503 | Msa1082370:intron | 25.0% |
| !!! | TTATGAACTACTTTTGTTGT+AGG | + | chr7_2:82465497-82465516 | Msa1082360:intron | 25.0% |
| GTGTTACCGTTTGAAAAATA+TGG | + | chr7_2:82463254-82463273 | Msa1082360:exon | 30.0% | |
| !! | CCTCTTCCATATTTTTCAAA+CGG | - | chr7_2:82463263-82463282 | Msa1082370:three_prime_UTR | 30.0% |
| ! | GATGATTTAAGGTTACTTTC+TGG | + | chr7_2:82463297-82463316 | Msa1082360:CDS | 30.0% |
| ATAGAGATTGCATACAAAGA+TGG | + | chr7_2:82463554-82463573 | Msa1082360:CDS | 30.0% | |
| TAATCATCATCATACATACC+TGG | - | chr7_2:82463618-82463637 | Msa1082370:intron | 30.0% | |
| GGAAATGAAGATAGAACTAA+AGG | - | chr7_2:82463998-82464017 | Msa1082370:intron | 30.0% | |
| TTTATCTCTGATTTCTTTGG+AGG | - | chr7_2:82464192-82464211 | Msa1082370:three_prime_UTR | 30.0% | |
| !! | TTGATAACACATTTTAAGGG+GGG | + | chr7_2:82464473-82464492 | Msa1082360:intron | 30.0% |
| TGTAATAGAAAGTGATTACC+CGG | + | chr7_2:82464542-82464561 | Msa1082360:CDS | 30.0% | |
| !! | TCAAACTTTTAGGTCACATT+TGG | + | chr7_2:82465045-82465064 | Msa1082360:intron | 30.0% |
| ! | TCTTTTTAGACATGAAAGCA+TGG | - | chr7_2:82465148-82465167 | Msa1082370:intron | 30.0% |
| ! | CTTTAATTTAACCTGCAGTT+AGG | + | chr7_2:82465192-82465211 | Msa1082360:intron | 30.0% |
| GTTACTAGTAAGACATTGTT+TGG | + | chr7_2:82465337-82465356 | Msa1082360:CDS | 30.0% | |
| AAGTGTAAATGGGTAATCTT+CGG | - | chr7_2:82465381-82465400 | Msa1082370:three_prime_UTR | 30.0% | |
| ! | TTACGGTAAGAAGTGTAAAT+GGG | - | chr7_2:82465391-82465410 | Msa1082370:intron | 30.0% |
| TACATAGTTACCAAGACAAT+CGG | + | chr7_2:82465558-82465577 | Msa1082360:CDS | 30.0% | |
| CCGTTTGAAAAATATGGAAG+AGG | + | chr7_2:82463260-82463279 | Msa1082360:exon | 35.0% | |
| AGCTTTCAAACTCTTCTTCA+TGG | - | chr7_2:82463344-82463363 | Msa1082370:three_prime_UTR | 35.0% | |
| !! | TTCTTGTTCTGGAACTGTTA+AGG | + | chr7_2:82463386-82463405 | Msa1082360:CDS | 35.0% |
| AACATGTGAAATATGTCTCC+AGG | + | chr7_2:82463597-82463616 | Msa1082360:CDS | 35.0% | |
| ATGTTGTGACGTAAATTGGA+TGG | + | chr7_2:82463817-82463836 | Msa1082360:intron | 35.0% | |
| TGTTGTGACGTAAATTGGAT+GGG | + | chr7_2:82463818-82463837 | Msa1082360:intron | 35.0% | |
| ! | TTTTTGCAGCAATATGAACC+TGG | + | chr7_2:82464153-82464172 | Msa1082360:intron | 35.0% |
| TATCAAGTCTTAGCTACACA+TGG | + | chr7_2:82464302-82464321 | Msa1082360:intron | 35.0% | |
| ! | GGTGCAACTATTTTATACGT+TGG | + | chr7_2:82464331-82464350 | Msa1082360:intron | 35.0% |
| TTAGGGAAAATAACGAGTCA+AGG | + | chr7_2:82464752-82464771 | Msa1082360:intron | 35.0% | |
| TAGGGAAAATAACGAGTCAA+GGG | + | chr7_2:82464753-82464772 | Msa1082360:intron | 35.0% | |
| CTGAACTACTAGTACATAGT+CGG | - | chr7_2:82465098-82465117 | Msa1082370:intron | 35.0% | |
| GAAGCTGGTCAAATTAAAAC+TGG | + | chr7_2:82465234-82465253 | Msa1082360:intron | 35.0% | |
| CATGCAATGATGTATTCTGT+AGG | + | chr7_2:82465267-82465286 | Msa1082360:intron | 35.0% | |
| ! | CTTACGGTAAGAAGTGTAAA+TGG | - | chr7_2:82465392-82465411 | Msa1082370:intron | 35.0% |
| CGGAAATCAAGTACTACTTA+CGG | - | chr7_2:82465408-82465427 | Msa1082370:intron | 35.0% | |
| GTTGTAGGTCATTGTTCTAA+AGG | + | chr7_2:82465512-82465531 | Msa1082360:intron | 35.0% | |
| ! | GTCTTGGTAACTATGTACAT+GGG | - | chr7_2:82465555-82465574 | Msa1082370:three_prime_UTR | 35.0% |
| ! | TGTCTTGGTAACTATGTACA+TGG | - | chr7_2:82465556-82465575 | Msa1082370:three_prime_UTR | 35.0% |
| ! | ACAAGAACAAGGTGCTTCTA+AGG | - | chr7_2:82463373-82463392 | Msa1082370:three_prime_UTR | 40.0% |
| ACAGTTCCAGAACAAGAACA+AGG | - | chr7_2:82463384-82463403 | Msa1082370:three_prime_UTR | 40.0% | |
| CAAAGATGGTGTAACGAGAA+AGG | + | chr7_2:82463568-82463587 | Msa1082360:CDS | 40.0% | |
| !! | ACACGTATTTTGCTGTCGTT+AGG | + | chr7_2:82463790-82463809 | Msa1082360:intron | 40.0% |
| ! | ATCTCTGATTTCTTTGGAGG+AGG | - | chr7_2:82464189-82464208 | Msa1082370:three_prime_UTR | 40.0% |
| ATGATGAAGCAATGCCCATA+AGG | + | chr7_2:82464211-82464230 | Msa1082360:CDS | 40.0% | |
| ! | AACGCGGTAAAGTACCTTAT+GGG | - | chr7_2:82464228-82464247 | Msa1082370:intron | 40.0% |
| ! | AAACGCGGTAAAGTACCTTA+TGG | - | chr7_2:82464229-82464248 | Msa1082370:intron | 40.0% |
| AAACGCGAAAAGAGAAAGTG+AGG | + | chr7_2:82464509-82464528 | Msa1082360:CDS | 40.0% | |
| AAAAGAGAAAGTGAGGTGGA+AGG | + | chr7_2:82464516-82464535 | Msa1082360:CDS | 40.0% | |
| TGGCAATGACAATTCAACTC+AGG | - | chr7_2:82464780-82464799 | Msa1082370:intron | 40.0% | |
| GTTGAATTGTCATTGCCAAC+TGG | + | chr7_2:82464782-82464801 | Msa1082360:intron | 40.0% | |
| !!! | ACCTTGTGAAAGCATTTTCC+TGG | + | chr7_2:82464883-82464902 | Msa1082360:intron | 40.0% |
| ACCAGGAAAATGCTTTCACA+AGG | - | chr7_2:82464887-82464906 | Msa1082370:intron | 40.0% | |
| ! | CATGAAAGCATGGTGTTTGT+GGG | - | chr7_2:82465138-82465157 | Msa1082370:intron | 40.0% |
| ! | ACATGAAAGCATGGTGTTTG+TGG | - | chr7_2:82465139-82465158 | Msa1082370:intron | 40.0% |
| TTCCCAACTGATATTGAAGC+TGG | + | chr7_2:82465219-82465238 | Msa1082360:intron | 40.0% | |
| GACCAGCTTCAATATCAGTT+GGG | - | chr7_2:82465224-82465243 | Msa1082370:intron | 40.0% | |
| TGACCAGCTTCAATATCAGT+TGG | - | chr7_2:82465225-82465244 | Msa1082370:intron | 40.0% | |
| TAATCTTCGGTTCCACTTGT+GGG | - | chr7_2:82465368-82465387 | Msa1082370:three_prime_UTR | 40.0% | |
| !! | GTCATTGTTCTAAAGGCTTG+CGG | + | chr7_2:82465519-82465538 | Msa1082360:CDS | 40.0% |
| GAGCCATTCTGAATAGCATT+AGG | + | chr7_2:82465580-82465599 | Msa1082360:CDS | 40.0% | |
| AATAGCATTAGGCGCTATCA+AGG | + | chr7_2:82465591-82465610 | Msa1082360:CDS | 40.0% | |
| ACATCACTCTCTGATGAAGA+TGG | + | chr7_2:82465627-82465646 | Msa1082360:CDS | 40.0% | |
| TAATGGCAGAAATGAAGAGC+AGG | + | chr7_2:82465699-82465718 | Msa1082360:three_prime_UTR | 40.0% | |
| !! | ACAAGGTGCTTCTAAGGCTT+TGG | - | chr7_2:82463367-82463386 | Msa1082370:three_prime_UTR | 45.0% |
| ! | GAAGCACCTTGTTCTTGTTC+TGG | + | chr7_2:82463375-82463394 | Msa1082360:CDS | 45.0% |
| GGGAGAAAGAGTTATCTGTG+AGG | + | chr7_2:82463767-82463786 | Msa1082360:intron | 45.0% | |
| AGTCTTAGCTACACATGGCA+TGG | + | chr7_2:82464307-82464326 | Msa1082360:intron | 45.0% | |
| TTAGTGGTAGAGCTGCATTC+CGG | - | chr7_2:82464564-82464583 | Msa1082370:three_prime_UTR | 45.0% | |
| CAATGATCGACAGTGAAACG+CGG | - | chr7_2:82464592-82464611 | Msa1082370:three_prime_UTR | 45.0% | |
| TTGGGAAAAACCCTAACTGC+AGG | - | chr7_2:82465206-82465225 | Msa1082370:intron | 45.0% | |
| GTAATCTTCGGTTCCACTTG+TGG | - | chr7_2:82465369-82465388 | Msa1082370:three_prime_UTR | 45.0% | |
| CCATTAGTACGGCTGTATGT+AGG | - | chr7_2:82465473-82465492 | Msa1082370:intron | 45.0% | |
| CCTACATACAGCCGTACTAA+TGG | + | chr7_2:82465470-82465489 | Msa1082360:intron | 45.0% | |
| GCGCCTAATGCTATTCAGAA+TGG | - | chr7_2:82465586-82465605 | Msa1082370:three_prime_UTR | 45.0% | |
| AGATGGCAGAAATGAAGAGC+AGG | + | chr7_2:82465644-82465663 | Msa1082360:CDS | 45.0% | |
| CTTAGCTACACATGGCATGG+AGG | + | chr7_2:82464310-82464329 | Msa1082360:intron | 50.0% | |
| CGCGAAAAGAGAAAGTGAGG+TGG | + | chr7_2:82464512-82464531 | Msa1082360:CDS | 50.0% | |
| TAGTGGTAGAGCTGCATTCC+GGG | - | chr7_2:82464563-82464582 | Msa1082370:three_prime_UTR | 50.0% | |
| GTGAAACGCGGTTCTGTTAG+TGG | - | chr7_2:82464580-82464599 | Msa1082370:three_prime_UTR | 50.0% | |
| CGCGTTTCACTGTCGATCAT+TGG | + | chr7_2:82464590-82464609 | Msa1082360:CDS | 50.0% | |
| ATGCCAATTGCCCCAACACA+AGG | + | chr7_2:82464806-82464825 | Msa1082360:intron | 50.0% | |
| TGCCAATTGCCCCAACACAA+GGG | + | chr7_2:82464807-82464826 | Msa1082360:intron | 50.0% | |
| ! | TTCTAGGAGCCCTTGTGTTG+GGG | - | chr7_2:82464819-82464838 | Msa1082370:intron | 50.0% |
| ! | CTTCTAGGAGCCCTTGTGTT+GGG | - | chr7_2:82464820-82464839 | Msa1082370:intron | 50.0% |
| ! | TCTTCTAGGAGCCCTTGTGT+TGG | - | chr7_2:82464821-82464840 | Msa1082370:intron | 50.0% |
| !! | TTTTGGGAGAGTCGTGTACC+AGG | - | chr7_2:82464904-82464923 | Msa1082370:intron | 50.0% |
| !! | TGTTTGGTAGTGCCCACAAG+TGG | + | chr7_2:82465353-82465372 | Msa1082360:CDS | 50.0% |
| CAGAATGGCTCCGATTGTCT+TGG | - | chr7_2:82465571-82465590 | Msa1082370:three_prime_UTR | 50.0% | |
| GGAGGAGGAGCTGTATATCC+AGG | - | chr7_2:82464174-82464193 | Msa1082370:three_prime_UTR | 55.0% | |
| GGGGCAATTGGCATTCCAGT+TGG | - | chr7_2:82464800-82464819 | Msa1082370:intron | 55.0% | |
| AGCCCTTGTGTTGGGGCAAT+TGG | - | chr7_2:82464812-82464831 | Msa1082370:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 82463179 | 82465779 | 82463179 | ID=Msa1082360;Name=Msa1082360 |
| chr7_2 | mRNA | 82463179 | 82465779 | 82463179 | ID=Msa1082360-mRNA-1;Parent=Msa1082360;Name=Msa1082360-mRNA-1;_AED=0.15;_eAED=0.15;_QI=94|1|1|1|1|1|6|87|220 |
| chr7_2 | exon | 82463179 | 82463407 | 82463179 | ID=Msa1082360-mRNA-1:exon:21321;Parent=Msa1082360-mRNA-1 |
| chr7_2 | exon | 82463547 | 82463618 | 82463547 | ID=Msa1082360-mRNA-1:exon:21322;Parent=Msa1082360-mRNA-1 |
| chr7_2 | exon | 82464162 | 82464232 | 82464162 | ID=Msa1082360-mRNA-1:exon:21323;Parent=Msa1082360-mRNA-1 |
| chr7_2 | exon | 82464491 | 82464620 | 82464491 | ID=Msa1082360-mRNA-1:exon:21324;Parent=Msa1082360-mRNA-1 |
| chr7_2 | exon | 82465326 | 82465406 | 82465326 | ID=Msa1082360-mRNA-1:exon:21325;Parent=Msa1082360-mRNA-1 |
| chr7_2 | exon | 82465519 | 82465779 | 82465519 | ID=Msa1082360-mRNA-1:exon:21326;Parent=Msa1082360-mRNA-1 |
| chr7_2 | five_prime_UTR | 82463179 | 82463272 | 82463179 | ID=Msa1082360-mRNA-1:five_prime_utr;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82463273 | 82463407 | 82463273 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82463547 | 82463618 | 82463547 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82464162 | 82464232 | 82464162 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82464491 | 82464620 | 82464491 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82465326 | 82465406 | 82465326 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | CDS | 82465519 | 82465692 | 82465519 | ID=Msa1082360-mRNA-1:cds;Parent=Msa1082360-mRNA-1 |
| chr7_2 | three_prime_UTR | 82465693 | 82465779 | 82465693 | ID=Msa1082360-mRNA-1:three_prime_utr;Parent=Msa1082360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
