Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045180 | XP_013447974.1 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.10e-40 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045180 | I3SYL4 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 5.27e-41 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0085960 | Msa1045180 | 0.814121 | 1.810645e-51 | -8.615850e-47 |
| Msa0700650 | Msa1045180 | 0.808454 | 3.072889e-50 | -8.615850e-47 |
| Msa0721280 | Msa1045180 | 0.800600 | 1.335997e-48 | -8.615850e-47 |
| Msa0773590 | Msa1045180 | 0.803609 | 3.212210e-49 | -8.615850e-47 |
| Msa0143540 | Msa1045180 | 0.985320 | 3.356021e-163 | -8.615850e-47 |
| Msa0170570 | Msa1045180 | 0.815861 | 7.441907e-52 | -8.615850e-47 |
| Msa0237410 | Msa1045180 | 0.806632 | 7.486688e-50 | -8.615850e-47 |
| Msa1045180 | Msa1087980 | 0.802295 | 6.004157e-49 | -8.615850e-47 |
| Msa1045180 | Msa1094190 | 0.802506 | 5.431018e-49 | -8.615850e-47 |
| Msa1045180 | Msa1353470 | 0.814005 | 1.920274e-51 | -8.615850e-47 |
| Msa1045180 | Msa1398090 | 0.801257 | 9.806976e-49 | -8.615850e-47 |
| Msa0259500 | Msa1045180 | 0.806741 | 7.098391e-50 | -8.615850e-47 |
| Msa0305700 | Msa1045180 | 0.816301 | 5.934358e-52 | -8.615850e-47 |
| Msa0334360 | Msa1045180 | 0.805520 | 1.283009e-49 | -8.615850e-47 |
| Msa0341540 | Msa1045180 | 0.806571 | 7.710289e-50 | -8.615850e-47 |
| Msa0572420 | Msa1045180 | 0.802541 | 5.342243e-49 | -8.615850e-47 |
| Msa0957870 | Msa1045180 | 0.801682 | 8.023757e-49 | -8.615850e-47 |
| Msa0974060 | Msa1045180 | 0.806115 | 9.620734e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045180 | MtrunA17_Chr7g0225151 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.01e-44 | 137 |
| Msa1045180 | MtrunA17_Chr7g0225011 | 47.761 | 67 | 35 | 0 | 1 | 67 | 1 | 67 | 8.23e-19 | 72.4 |
| Msa1045180 | MtrunA17_Chr8g0386301 | 44.776 | 67 | 37 | 0 | 1 | 67 | 1 | 67 | 2.28e-18 | 71.2 |
| Msa1045180 | MtrunA17_Chr3g0122331 | 46.970 | 66 | 35 | 0 | 1 | 66 | 1 | 66 | 2.63e-18 | 70.9 |
| Msa1045180 | MtrunA17_Chr8g0377511 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 3.65e-18 | 70.5 |
| Msa1045180 | MtrunA17_Chr7g0224981 | 45.455 | 66 | 36 | 0 | 1 | 66 | 1 | 66 | 1.15e-16 | 67.0 |
| Msa1045180 | MtrunA17_Chr7g0224941 | 45.455 | 66 | 36 | 0 | 1 | 66 | 1 | 66 | 1.15e-16 | 67.0 |
| Msa1045180 | MtrunA17_Chr8g0347211 | 39.706 | 68 | 41 | 0 | 1 | 68 | 1 | 68 | 2.13e-16 | 66.2 |
| Msa1045180 | MtrunA17_Chr3g0122341 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 2.15e-16 | 66.2 |
| Msa1045180 | MtrunA17_Chr7g0225171 | 38.806 | 67 | 41 | 0 | 1 | 67 | 1 | 67 | 6.28e-15 | 62.4 |
| Msa1045180 | MtrunA17_Chr7g0225041 | 38.462 | 65 | 40 | 0 | 3 | 67 | 4 | 68 | 2.05e-14 | 61.2 |
| Msa1045180 | MtrunA17_Chr7g0225141 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 4.86e-14 | 60.1 |
| Msa1045180 | MtrunA17_Chr7g0225061 | 35.385 | 65 | 42 | 0 | 3 | 67 | 4 | 68 | 1.91e-13 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045180 | AT5G40960.1 | 71.642 | 67 | 19 | 0 | 1 | 67 | 1 | 67 | 4.54e-25 | 88.6 |
| Msa1045180 | AT5G08391.1 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 3.21e-17 | 68.6 |
| Msa1045180 | AT3G48660.1 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 3.90e-17 | 68.9 |
| Msa1045180 | AT5G63500.1 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 2.36e-16 | 66.2 |
| Msa1045180 | AT3G27027.1 | 37.879 | 66 | 41 | 0 | 2 | 67 | 3 | 68 | 2.74e-14 | 61.2 |
| Msa1045180 | AT5G14110.1 | 37.879 | 66 | 41 | 0 | 3 | 68 | 4 | 69 | 1.53e-13 | 59.3 |
| Msa1045180 | AT5G40980.1 | 51.020 | 49 | 24 | 0 | 3 | 51 | 4 | 52 | 1.96e-13 | 58.9 |
| Msa1045180 | AT3G27030.1 | 40.299 | 67 | 40 | 0 | 1 | 67 | 74 | 140 | 2.28e-13 | 60.8 |
| Msa1045180 | AT3G27030.1 | 36.364 | 66 | 42 | 0 | 1 | 66 | 1 | 66 | 1.41e-11 | 56.2 |
| Msa1045180 | AT3G01950.1 | 38.462 | 65 | 40 | 0 | 3 | 67 | 4 | 68 | 6.99e-13 | 57.8 |
Find 9 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTACACACTGTTCTTTA+TGG | 0.216202 | 7_2:+4391947 | Msa1045180:CDS |
| AGGGATAGACAATGAATAAT+TGG | 0.218789 | 7_2:+4391804 | None:intergenic |
| TCATAGCTGCAGCTCTATTT+TGG | 0.248610 | 7_2:+4391837 | Msa1045180:CDS |
| GGGATAGACAATGAATAATT+GGG | 0.433197 | 7_2:+4391805 | None:intergenic |
| TCCAATGGTGAATTCTTCCC+TGG | 0.455815 | 7_2:-4391890 | None:intergenic |
| TCCAGGGAAGAATTCACCAT+TGG | 0.544316 | 7_2:+4391889 | Msa1045180:CDS |
| TTCATATTCATAAACTCCAA+TGG | 0.593728 | 7_2:-4391905 | None:intergenic |
| AATAGAGCTGCAGCTATGAG+AGG | 0.636515 | 7_2:-4391833 | None:intergenic |
| AGAGCTGCAGCTATGAGAGG+AGG | 0.659838 | 7_2:-4391830 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCATATTCATAAACTCCAA+TGG | - | chr7_2:4391908-4391927 | None:intergenic | 25.0% |
| TTTGTACACACTGTTCTTTA+TGG | + | chr7_2:4391947-4391966 | Msa1045180:CDS | 30.0% | |
| !!! | CTATTTTGGCTATTATCACC+AGG | + | chr7_2:4391851-4391870 | Msa1045180:CDS | 35.0% |
| !! | GAATGATTTTTCAGCTTCCA+GGG | + | chr7_2:4391873-4391892 | Msa1045180:CDS | 35.0% |
| ! | TCATAGCTGCAGCTCTATTT+TGG | + | chr7_2:4391837-4391856 | Msa1045180:CDS | 40.0% |
| GGAAGCTGAAAAATCATTCC+TGG | - | chr7_2:4391872-4391891 | None:intergenic | 40.0% | |
| !! | GGAATGATTTTTCAGCTTCC+AGG | + | chr7_2:4391872-4391891 | Msa1045180:CDS | 40.0% |
| AATAGAGCTGCAGCTATGAG+AGG | - | chr7_2:4391836-4391855 | None:intergenic | 45.0% | |
| TCCAGGGAAGAATTCACCAT+TGG | + | chr7_2:4391889-4391908 | Msa1045180:CDS | 45.0% | |
| TCCAATGGTGAATTCTTCCC+TGG | - | chr7_2:4391893-4391912 | None:intergenic | 45.0% | |
| AGAGCTGCAGCTATGAGAGG+AGG | - | chr7_2:4391833-4391852 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 4391815 | 4392024 | 4391815 | ID=Msa1045180;Name=Msa1045180 |
| chr7_2 | mRNA | 4391815 | 4392024 | 4391815 | ID=Msa1045180-mRNA-1;Parent=Msa1045180;Name=Msa1045180-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|69 |
| chr7_2 | exon | 4391815 | 4392024 | 4391815 | ID=Msa1045180-mRNA-1:exon:1044;Parent=Msa1045180-mRNA-1 |
| chr7_2 | CDS | 4391815 | 4392024 | 4391815 | ID=Msa1045180-mRNA-1:cds;Parent=Msa1045180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
