AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1136550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136550 MtrunA17_Chr7g0218101 98.282 291 5 0 1 291 1 291 0.0 589
Msa1136550 MtrunA17_Chr3g0077201 43.825 251 118 6 49 288 83 321 1.52e-57 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136550 AT3G29240.1 61.736 311 93 7 1 286 1 310 1.05e-121 351
Msa1136550 AT3G29240.2 61.736 311 93 7 1 286 1 310 1.05e-121 351
Msa1136550 AT1G33780.1 46.983 232 104 4 59 286 95 311 9.90e-56 183
Msa1136550 AT3G19780.1 32.258 155 87 5 114 259 904 1049 3.35e-12 67.4
Msa1136550 AT3G19780.2 32.258 155 87 5 114 259 903 1048 3.71e-12 67.0
Msa1136550 AT3G19780.4 32.258 155 87 5 114 259 712 857 4.17e-12 67.0
Msa1136550 AT3G19780.3 32.258 155 87 5 114 259 694 839 4.34e-12 67.0

Find 131 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATAGTCCTTTGTATTT+TGG 0.063786 7_4:+5196726 Msa1136550:CDS
TGAAATTGCATCGCATATTT+TGG 0.164391 7_4:+5197649 Msa1136550:three_prime_UTR
ATTAATAAACAACCTCTTTC+AGG 0.170100 7_4:-5196543 None:intergenic
TTCAAGTTACTACTCAAATT+AGG 0.182399 7_4:-5197416 None:intergenic
GATTCAATTTCAGTGTAATT+TGG 0.212433 7_4:+5197673 Msa1136550:three_prime_UTR
AACAATTGAGAGATGAAATT+AGG 0.215233 7_4:+5196949 Msa1136550:CDS
TTCGGCTATGTTTGAATTGA+TGG 0.252184 7_4:+5197288 Msa1136550:three_prime_UTR
TTGGGGAGTGTTGGAATTGT+TGG 0.255889 7_4:+5197017 Msa1136550:CDS
GGACGAAGTTCTCGGGCTTA+TGG 0.259316 7_4:+5197046 Msa1136550:CDS
GTGTTGGAATTGTTGGGCTT+TGG 0.264579 7_4:+5197024 Msa1136550:CDS
ATGAAATTAGGGATGGTTAT+TGG 0.278275 7_4:+5196961 Msa1136550:CDS
TTTCCCACCCCATCATCTTT+AGG 0.290799 7_4:-5196783 None:intergenic
AGTCCAAGTGTTGTTGATTT+GGG 0.292790 7_4:+5196999 Msa1136550:CDS
TGTTGGAATTGTTGGGCTTT+GGG 0.307524 7_4:+5197025 Msa1136550:CDS
GATAAAACAATGTTTATGTT+TGG 0.310889 7_4:+5197165 Msa1136550:three_prime_UTR
AACAAGTTACTCAAATAATA+TGG 0.318535 7_4:+5195450 Msa1136550:exon
TCAAGTTACTACTCAAATTA+GGG 0.318663 7_4:-5197415 None:intergenic
GTGATGAAGGGTTTGTATTA+TGG 0.321373 7_4:+5196825 Msa1136550:CDS
TAACTTGAAAACAACTTGTT+TGG 0.324966 7_4:+5197430 Msa1136550:three_prime_UTR
ACAATTGAGAGATGAAATTA+GGG 0.325075 7_4:+5196950 Msa1136550:CDS
TGGGGAGTGTTGGAATTGTT+GGG 0.327293 7_4:+5197018 Msa1136550:CDS
ACAACTTGTTTGGAAGCTAT+TGG 0.331561 7_4:+5197440 Msa1136550:three_prime_UTR
ATGATCCCTGATGGGCCTAT+TGG 0.337536 7_4:-5196633 None:intergenic
AAGATCGCCAATCGGTGTTA+AGG 0.343777 7_4:-5196386 None:intergenic
GACGAAGTTCTCGGGCTTAT+GGG 0.344877 7_4:+5197047 Msa1136550:CDS
TAGTCCAAGTGTTGTTGATT+TGG 0.347343 7_4:+5196998 Msa1136550:CDS
GGGCTTTGGGACGAAGTTCT+CGG 0.349513 7_4:+5197038 Msa1136550:CDS
TCTTCATCTCATCCAAAGTT+TGG 0.355676 7_4:-5195307 None:intergenic
GATGTTAACTATGAGAATTT+TGG 0.357996 7_4:+5197568 Msa1136550:three_prime_UTR
ATCAACTGCATTTGATGTAA+TGG 0.365972 7_4:+5196695 Msa1136550:CDS
GTCACTGAAGAAAGGAATTC+GGG 0.366829 7_4:-5196447 None:intergenic
AACGTGAGGGTGGGTAAAAT+CGG 0.381314 7_4:-5197107 None:intergenic
TAATCACCATTGGTGACAAA+TGG 0.385718 7_4:+5196502 Msa1136550:CDS
AACAACTAAACACAACAATT+AGG 0.390992 7_4:-5197541 None:intergenic
AGTCACTGAAGAAAGGAATT+CGG 0.391310 7_4:-5196448 None:intergenic
AGTTTGGTGGAATATGTATA+TGG 0.391750 7_4:-5195388 None:intergenic
GGGCACTAGTATTAACCCTT+AGG 0.394769 7_4:-5197395 None:intergenic
TTGGTGTGTATTAAAATGTA+TGG 0.399837 7_4:-5195288 None:intergenic
GCGATCTTTCCGTGCAAAAC+TGG 0.403195 7_4:+5196401 Msa1136550:CDS
TGATCCCTGATGGGCCTATT+GGG 0.409481 7_4:-5196632 None:intergenic
ATCTTACATGATACATGAAA+TGG 0.417300 7_4:-5195580 None:intergenic
TATTAAAATGTATGGGCAAA+TGG 0.420446 7_4:-5195280 None:intergenic
GGCTTTGGGACGAAGTTCTC+GGG 0.421603 7_4:+5197039 Msa1136550:CDS
CTTTGATGGGTATTGTGGGT+GGG 0.424635 7_4:+5196920 Msa1136550:CDS
CAATTCAATTCAAGAAAAGT+TGG 0.426906 7_4:+5195553 Msa1136550:CDS
CTCCCCAAATCAACAACACT+TGG 0.428448 7_4:-5197002 None:intergenic
AACAACCTCTTTCAGGCTCA+TGG 0.428547 7_4:-5196536 None:intergenic
AATAAGCTTGTAGAAGATCT+AGG 0.431841 7_4:+5197358 Msa1136550:three_prime_UTR
TTGGTGAGTCCTAAAGATGA+TGG 0.434738 7_4:+5196774 Msa1136550:CDS
AATGGATATGCTTTGACAAA+TGG 0.440024 7_4:-5195262 None:intergenic
TTGTTGATGAGAAAATCTTT+TGG 0.440379 7_4:-5195533 None:intergenic
TCAACTGCATTTGATGTAAT+GGG 0.441472 7_4:+5196696 Msa1136550:CDS
AGATTCTTTGATGGGTATTG+TGG 0.441919 7_4:+5196915 Msa1136550:CDS
AGCAGATACTTCCATCCATC+AGG 0.442402 7_4:-5197616 None:intergenic
CCTTTCTTCAGTGACTAATC+CGG 0.443616 7_4:+5196455 Msa1136550:CDS
TGTGGGTCTTGCTGCTGAAA+TGG 0.445492 7_4:+5196860 Msa1136550:CDS
ATGTTTGAATTGATGGCATA+TGG 0.452478 7_4:+5197295 Msa1136550:three_prime_UTR
ATATGATGAGAGATCAAGTT+TGG 0.453059 7_4:-5195404 None:intergenic
GATTCTTTGATGGGTATTGT+GGG 0.454997 7_4:+5196916 Msa1136550:CDS
CTTGTGGGGATGGGAAAAGT+TGG 0.458568 7_4:+5197206 Msa1136550:three_prime_UTR
TAGAGATCCACCTCTGATCT+TGG 0.458828 7_4:-5195503 None:intergenic
AGATCGCCAATCGGTGTTAA+GGG 0.458925 7_4:-5196385 None:intergenic
CGCCAATCGGTGTTAAGGGT+TGG 0.469028 7_4:-5196381 None:intergenic
GGTGTAGTAGCAAGACCCTA+AGG 0.472149 7_4:+5197379 Msa1136550:three_prime_UTR
ATGGAGAGTGCTTGTGGGGA+TGG 0.476529 7_4:+5197196 Msa1136550:three_prime_UTR
TTCAAAATGTCTAATCCTGA+TGG 0.476620 7_4:+5197601 Msa1136550:three_prime_UTR
TTTGGAAGCTATTGGCAAGC+AGG 0.478143 7_4:+5197448 Msa1136550:three_prime_UTR
GGATGATCCACAACAGTGTC+CGG 0.482240 7_4:-5196474 None:intergenic
TCTTTGATGGGTATTGTGGG+TGG 0.484182 7_4:+5196919 Msa1136550:CDS
TTGTGGGGATGGGAAAAGTT+GGG 0.487517 7_4:+5197207 Msa1136550:three_prime_UTR
TTGGGCCGTTTGATAAAAGA+AGG 0.489401 7_4:-5196614 None:intergenic
AATCACCATTGGTGACAAAT+GGG 0.489815 7_4:+5196503 Msa1136550:CDS
GGCACTAGTATTAACCCTTA+GGG 0.491646 7_4:-5197394 None:intergenic
GGGCTTTAGTAATTGTTCTC+CGG 0.495969 7_4:-5196427 None:intergenic
TAGCAACAGAAAAGCTTGAT+GGG 0.501175 7_4:+5196565 Msa1136550:CDS
TTGTCACCAATGGTGATTAG+TGG 0.504517 7_4:-5196498 None:intergenic
ATAGCAACAGAAAAGCTTGA+TGG 0.509500 7_4:+5196564 Msa1136550:CDS
TTCTCGGGCTTATGGGCAAA+AGG 0.513225 7_4:+5197054 Msa1136550:CDS
ACGTGAGGGTGGGTAAAATC+GGG 0.514202 7_4:-5197106 None:intergenic
TGCACGGAAAGATCGCCAAT+CGG 0.515533 7_4:-5196394 None:intergenic
AACCAACCCTTAACACCGAT+TGG 0.518296 7_4:+5196379 Msa1136550:CDS
TCTTTGATAGACATCAAAGA+TGG 0.519470 7_4:-5196666 None:intergenic
TACACATGGAGAGTGCTTGT+GGG 0.520693 7_4:+5197191 Msa1136550:three_prime_UTR
GTTGTTGATTTGGGGAGTGT+TGG 0.528597 7_4:+5197008 Msa1136550:CDS
AAGAGGAATGTGGTTGAGGT+TGG 0.532156 7_4:+5196885 Msa1136550:CDS
TTCCGTGCAAAACTGGTGGC+CGG 0.536600 7_4:+5196408 Msa1136550:CDS
CCGGATTAGTCACTGAAGAA+AGG 0.540072 7_4:-5196455 None:intergenic
GGCTCTAATTGAAAGGGTAC+CGG 0.542888 7_4:+5197228 Msa1136550:three_prime_UTR
TGGAGAGTGCTTGTGGGGAT+GGG 0.546406 7_4:+5197197 Msa1136550:three_prime_UTR
TGGTGAGTCCTAAAGATGAT+GGG 0.547996 7_4:+5196775 Msa1136550:CDS
AAAGTTGGGCTCTAATTGAA+AGG 0.551613 7_4:+5197221 Msa1136550:three_prime_UTR
TGATGAGAGATCAAGTTTGG+TGG 0.559897 7_4:-5195401 None:intergenic
AAATGTCTAATCCTGATGGA+TGG 0.564405 7_4:+5197605 Msa1136550:three_prime_UTR
ATACACATGGAGAGTGCTTG+TGG 0.564493 7_4:+5197190 Msa1136550:three_prime_UTR
CTATTGAGTATGATCCCTGA+TGG 0.565216 7_4:-5196642 None:intergenic
ATCTTTCCGTGCAAAACTGG+TGG 0.565955 7_4:+5196404 Msa1136550:CDS
GTGAGCCCATTTGTCACCAA+TGG 0.567698 7_4:-5196508 None:intergenic
TTGCTGCTGAAATGGTAAAG+AGG 0.567807 7_4:+5196868 Msa1136550:CDS
GTAATCCATGAGCCTGAAAG+AGG 0.568190 7_4:+5196531 Msa1136550:CDS
GACTAATCCGGACACTGTTG+TGG 0.573025 7_4:+5196467 Msa1136550:CDS
TGGTGTGTATTAAAATGTAT+GGG 0.573781 7_4:-5195287 None:intergenic
AAGTTGGGCTCTAATTGAAA+GGG 0.573938 7_4:+5197222 Msa1136550:three_prime_UTR
CGGGCTTATGGGCAAAAGGA+AGG 0.575753 7_4:+5197058 Msa1136550:CDS
GTCCAAGTGTTGTTGATTTG+GGG 0.578640 7_4:+5197000 Msa1136550:CDS
GGTAAAGAGGAATGTGGTTG+AGG 0.579733 7_4:+5196881 Msa1136550:CDS
TATTGAGTATGATCCCTGAT+GGG 0.580509 7_4:-5196641 None:intergenic
TTTCGAGGAAACGTGAGGGT+GGG 0.587065 7_4:-5197116 None:intergenic
TCCACCTCTGATCTTGGTGA+AGG 0.588907 7_4:-5195497 None:intergenic
TCCTTCACCAAGATCAGAGG+TGG 0.589140 7_4:+5195496 Msa1136550:CDS
TTGAGAGATGAAATTAGGGA+TGG 0.595243 7_4:+5196954 Msa1136550:CDS
CATCCACCACTAATCACCAT+TGG 0.598786 7_4:+5196492 Msa1136550:CDS
TTATGGTACTAAAGAAAGTG+TGG 0.602423 7_4:+5196842 Msa1136550:CDS
CATGTCATCATCATCACCAT+CGG 0.602474 7_4:+5196347 Msa1136550:CDS
TCACCAATGGTGATTAGTGG+TGG 0.603012 7_4:-5196495 None:intergenic
TTGTTCTTTAGATGAGAGTG+AGG 0.607062 7_4:-5195432 None:intergenic
TTTATGTTTGGAAATACACA+TGG 0.610879 7_4:+5197177 Msa1136550:three_prime_UTR
GAGAACATGAGTGTCAATAC+CGG 0.618757 7_4:-5197247 None:intergenic
TGCTTTGACAAATGGCACAT+AGG 0.619813 7_4:-5195254 None:intergenic
GTGTAGTAGCAAGACCCTAA+GGG 0.624591 7_4:+5197380 Msa1136550:three_prime_UTR
TATGGTACTAAAGAAAGTGT+GGG 0.628740 7_4:+5196843 Msa1136550:CDS
GAGTCCTAAAGATGATGGGG+TGG 0.634318 7_4:+5196779 Msa1136550:CDS
AGTTCCTTCACCAAGATCAG+AGG 0.637714 7_4:+5195493 Msa1136550:CDS
ACGGCCCAATAGGCCCATCA+GGG 0.647590 7_4:+5196628 Msa1136550:CDS
AGTCCTAAAGATGATGGGGT+GGG 0.647626 7_4:+5196780 Msa1136550:CDS
AGCAACAGAAAAGCTTGATG+GGG 0.650228 7_4:+5196566 Msa1136550:CDS
ACATACAAAATACTTCTATG+TGG 0.659206 7_4:+5195336 Msa1136550:five_prime_UTR
GGTGAGTCCTAAAGATGATG+GGG 0.678073 7_4:+5196776 Msa1136550:CDS
ACACATGGAGAGTGCTTGTG+GGG 0.684509 7_4:+5197192 Msa1136550:three_prime_UTR
GGCAAAAGGAAGGTCAAGTG+AGG 0.692062 7_4:+5197068 Msa1136550:CDS
TGAAATGGTAAAGAGGAATG+TGG 0.729562 7_4:+5196875 Msa1136550:CDS
ATGGTGATGATGATGACATG+TGG 0.732547 7_4:-5196344 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAAATATGTGAATT+TGG + chr7_4:5195995-5196014 Msa1136550:intron 10.0%
!! ACTTACTATATATAATTTAG+AGG - chr7_4:5195969-5195988 None:intergenic 15.0%
!!! TATATTGATAATGATACTTT+AGG + chr7_4:5196047-5196066 Msa1136550:intron 15.0%
!!! ATATTGATAATGATACTTTA+GGG + chr7_4:5196048-5196067 Msa1136550:intron 15.0%
!! TAAAAAAACAAATTTCTACA+TGG - chr7_4:5196106-5196125 None:intergenic 15.0%
!! AACAAGTTACTCAAATAATA+TGG + chr7_4:5195450-5195469 Msa1136550:exon 20.0%
!!! TATTGATAATGATACTTTAG+GGG + chr7_4:5196049-5196068 Msa1136550:intron 20.0%
!!! GAAATTTGTTTTTTTACCAT+TGG + chr7_4:5196110-5196129 Msa1136550:intron 20.0%
!! GATAAAACAATGTTTATGTT+TGG + chr7_4:5197165-5197184 Msa1136550:three_prime_UTR 20.0%
! TATTAAAATGTATGGGCAAA+TGG - chr7_4:5195283-5195302 None:intergenic 25.0%
! TGGTGTGTATTAAAATGTAT+GGG - chr7_4:5195290-5195309 None:intergenic 25.0%
! TTGGTGTGTATTAAAATGTA+TGG - chr7_4:5195291-5195310 None:intergenic 25.0%
!! TTTTAATACACACCAAACTT+TGG + chr7_4:5195295-5195314 Msa1136550:five_prime_UTR 25.0%
! ACATACAAAATACTTCTATG+TGG + chr7_4:5195336-5195355 Msa1136550:five_prime_UTR 25.0%
!!! TTGTTGATGAGAAAATCTTT+TGG - chr7_4:5195536-5195555 None:intergenic 25.0%
! CAATTCAATTCAAGAAAAGT+TGG + chr7_4:5195553-5195572 Msa1136550:CDS 25.0%
! ATCTTACATGATACATGAAA+TGG - chr7_4:5195583-5195602 None:intergenic 25.0%
! ATCTTGATCTTAGTATAAGT+AGG - chr7_4:5195615-5195634 None:intergenic 25.0%
! ATATAATTTAGAGGCTAAGT+TGG - chr7_4:5195960-5195979 None:intergenic 25.0%
! ATTAATAAACAACCTCTTTC+AGG - chr7_4:5196546-5196565 None:intergenic 25.0%
!! TCTAATAGTCCTTTGTATTT+TGG + chr7_4:5196726-5196745 Msa1136550:CDS 25.0%
!! CTAATAGTCCTTTGTATTTT+GGG + chr7_4:5196727-5196746 Msa1136550:CDS 25.0%
!! TAATAGTCCTTTGTATTTTG+GGG + chr7_4:5196728-5196747 Msa1136550:CDS 25.0%
!! GTGATTTTAGATTCTTTGAT+GGG + chr7_4:5196907-5196926 Msa1136550:CDS 25.0%
! AACAATTGAGAGATGAAATT+AGG + chr7_4:5196949-5196968 Msa1136550:CDS 25.0%
! ACAATTGAGAGATGAAATTA+GGG + chr7_4:5196950-5196969 Msa1136550:CDS 25.0%
! TTTATGTTTGGAAATACACA+TGG + chr7_4:5197177-5197196 Msa1136550:three_prime_UTR 25.0%
! TCAAGTTACTACTCAAATTA+GGG - chr7_4:5197418-5197437 None:intergenic 25.0%
!! TTCAAGTTACTACTCAAATT+AGG - chr7_4:5197419-5197438 None:intergenic 25.0%
!! TAACTTGAAAACAACTTGTT+TGG + chr7_4:5197430-5197449 Msa1136550:three_prime_UTR 25.0%
! AACAACTAAACACAACAATT+AGG - chr7_4:5197544-5197563 None:intergenic 25.0%
!! GATGTTAACTATGAGAATTT+TGG + chr7_4:5197568-5197587 Msa1136550:three_prime_UTR 25.0%
! GATTCAATTTCAGTGTAATT+TGG + chr7_4:5197673-5197692 Msa1136550:three_prime_UTR 25.0%
!!! AATTTTTTAGAGTCGTGTTA+AGG + chr7_4:5197725-5197744 Msa1136550:three_prime_UTR 25.0%
AATGGATATGCTTTGACAAA+TGG - chr7_4:5195265-5195284 None:intergenic 30.0%
! AGTTTGGTGGAATATGTATA+TGG - chr7_4:5195391-5195410 None:intergenic 30.0%
ATATGATGAGAGATCAAGTT+TGG - chr7_4:5195407-5195426 None:intergenic 30.0%
!!! TGAGAAAATCTTTTGGAGTA+AGG - chr7_4:5195529-5195548 None:intergenic 30.0%
TTAGTATAAGTAGGAGAAAG+AGG - chr7_4:5195606-5195625 None:intergenic 30.0%
GATGATACTTATTGATCCAA+TGG - chr7_4:5196129-5196148 None:intergenic 30.0%
TACATTAGAAAATGATGCAC+AGG - chr7_4:5196237-5196256 None:intergenic 30.0%
! GTGATCCTTCTTTTATCAAA+CGG + chr7_4:5196609-5196628 Msa1136550:CDS 30.0%
TCTTTGATAGACATCAAAGA+TGG - chr7_4:5196669-5196688 None:intergenic 30.0%
ATCAACTGCATTTGATGTAA+TGG + chr7_4:5196695-5196714 Msa1136550:CDS 30.0%
TCAACTGCATTTGATGTAAT+GGG + chr7_4:5196696-5196715 Msa1136550:CDS 30.0%
!! AATAGTCCTTTGTATTTTGG+GGG + chr7_4:5196729-5196748 Msa1136550:CDS 30.0%
AAAAACAACCCTTCTTCTAA+AGG - chr7_4:5196756-5196775 None:intergenic 30.0%
!! TTTAGAAGAAGGGTTGTTTT+TGG + chr7_4:5196755-5196774 Msa1136550:CDS 30.0%
!! TGTTTTTGATGAAGTGATGA+AGG + chr7_4:5196812-5196831 Msa1136550:CDS 30.0%
! GTTTTTGATGAAGTGATGAA+GGG + chr7_4:5196813-5196832 Msa1136550:CDS 30.0%
TTATGGTACTAAAGAAAGTG+TGG + chr7_4:5196842-5196861 Msa1136550:CDS 30.0%
! TATGGTACTAAAGAAAGTGT+GGG + chr7_4:5196843-5196862 Msa1136550:CDS 30.0%
! GGTGATTTTAGATTCTTTGA+TGG + chr7_4:5196906-5196925 Msa1136550:CDS 30.0%
ATGAAATTAGGGATGGTTAT+TGG + chr7_4:5196961-5196980 Msa1136550:CDS 30.0%
! AAGTGAGGAGCAATATTTTA+GGG + chr7_4:5197083-5197102 Msa1136550:exon 30.0%
! AAATCATGTGTGATTTTTCG+AGG - chr7_4:5197134-5197153 None:intergenic 30.0%
! ATGTTTGAATTGATGGCATA+TGG + chr7_4:5197295-5197314 Msa1136550:three_prime_UTR 30.0%
AATAAGCTTGTAGAAGATCT+AGG + chr7_4:5197358-5197377 Msa1136550:three_prime_UTR 30.0%
! AGGATTAGACATTTTGAACT+TGG - chr7_4:5197599-5197618 None:intergenic 30.0%
TTCAAAATGTCTAATCCTGA+TGG + chr7_4:5197601-5197620 Msa1136550:three_prime_UTR 30.0%
! TGAAATTGCATCGCATATTT+TGG + chr7_4:5197649-5197668 Msa1136550:three_prime_UTR 30.0%
TCTTCATCTCATCCAAAGTT+TGG - chr7_4:5195310-5195329 None:intergenic 35.0%
TTGTTCTTTAGATGAGAGTG+AGG - chr7_4:5195435-5195454 None:intergenic 35.0%
ATGCTGATATGTTCTCAAGA+TGG + chr7_4:5195780-5195799 Msa1136550:intron 35.0%
TCAATGCTGAGACTTATAGA+TGG + chr7_4:5196162-5196181 Msa1136550:intron 35.0%
! ATCTTTTGTGATCAAACCAC+AGG + chr7_4:5196318-5196337 Msa1136550:intron 35.0%
AGTCACTGAAGAAAGGAATT+CGG - chr7_4:5196451-5196470 None:intergenic 35.0%
! TAATCACCATTGGTGACAAA+TGG + chr7_4:5196502-5196521 Msa1136550:CDS 35.0%
! AATCACCATTGGTGACAAAT+GGG + chr7_4:5196503-5196522 Msa1136550:CDS 35.0%
ATAGCAACAGAAAAGCTTGA+TGG + chr7_4:5196564-5196583 Msa1136550:CDS 35.0%
TAGCAACAGAAAAGCTTGAT+GGG + chr7_4:5196565-5196584 Msa1136550:CDS 35.0%
TATTGAGTATGATCCCTGAT+GGG - chr7_4:5196644-5196663 None:intergenic 35.0%
GTGATGAAGGGTTTGTATTA+TGG + chr7_4:5196825-5196844 Msa1136550:CDS 35.0%
TGAAATGGTAAAGAGGAATG+TGG + chr7_4:5196875-5196894 Msa1136550:CDS 35.0%
! AGATTCTTTGATGGGTATTG+TGG + chr7_4:5196915-5196934 Msa1136550:CDS 35.0%
! GATTCTTTGATGGGTATTGT+GGG + chr7_4:5196916-5196935 Msa1136550:CDS 35.0%
TTGAGAGATGAAATTAGGGA+TGG + chr7_4:5196954-5196973 Msa1136550:CDS 35.0%
! TAGTCCAAGTGTTGTTGATT+TGG + chr7_4:5196998-5197017 Msa1136550:CDS 35.0%
!! AGTCCAAGTGTTGTTGATTT+GGG + chr7_4:5196999-5197018 Msa1136550:CDS 35.0%
! CAAGTGAGGAGCAATATTTT+AGG + chr7_4:5197082-5197101 Msa1136550:exon 35.0%
! AAAGTTGGGCTCTAATTGAA+AGG + chr7_4:5197221-5197240 Msa1136550:three_prime_UTR 35.0%
! AAGTTGGGCTCTAATTGAAA+GGG + chr7_4:5197222-5197241 Msa1136550:three_prime_UTR 35.0%
TTCGGCTATGTTTGAATTGA+TGG + chr7_4:5197288-5197307 Msa1136550:three_prime_UTR 35.0%
! ACAACTTGTTTGGAAGCTAT+TGG + chr7_4:5197440-5197459 Msa1136550:three_prime_UTR 35.0%
AAATGTCTAATCCTGATGGA+TGG + chr7_4:5197605-5197624 Msa1136550:three_prime_UTR 35.0%
TGCTTTGACAAATGGCACAT+AGG - chr7_4:5195257-5195276 None:intergenic 40.0%
TGATGAGAGATCAAGTTTGG+TGG - chr7_4:5195404-5195423 None:intergenic 40.0%
! ATGGTGATGATGATGACATG+TGG - chr7_4:5196347-5196366 None:intergenic 40.0%
CATGTCATCATCATCACCAT+CGG + chr7_4:5196347-5196366 Msa1136550:CDS 40.0%
!!! GTTGGTTTTTCATCATCCGA+TGG - chr7_4:5196366-5196385 None:intergenic 40.0%
! GGGCTTTAGTAATTGTTCTC+CGG - chr7_4:5196430-5196449 None:intergenic 40.0%
GTCACTGAAGAAAGGAATTC+GGG - chr7_4:5196450-5196469 None:intergenic 40.0%
CCTTTCTTCAGTGACTAATC+CGG + chr7_4:5196455-5196474 Msa1136550:CDS 40.0%
TTGTCACCAATGGTGATTAG+TGG - chr7_4:5196501-5196520 None:intergenic 40.0%
AGCAACAGAAAAGCTTGATG+GGG + chr7_4:5196566-5196585 Msa1136550:CDS 40.0%
! GGTTCACATTTTCGAACGAA+CGG + chr7_4:5196587-5196606 Msa1136550:CDS 40.0%
TTGGGCCGTTTGATAAAAGA+AGG - chr7_4:5196617-5196636 None:intergenic 40.0%
! CTTTTATCAAACGGCCCAAT+AGG + chr7_4:5196618-5196637 Msa1136550:CDS 40.0%
CTATTGAGTATGATCCCTGA+TGG - chr7_4:5196645-5196664 None:intergenic 40.0%
AAAGGTCCCCCAAAATACAA+AGG - chr7_4:5196738-5196757 None:intergenic 40.0%
TTGGTGAGTCCTAAAGATGA+TGG + chr7_4:5196774-5196793 Msa1136550:CDS 40.0%
TGGTGAGTCCTAAAGATGAT+GGG + chr7_4:5196775-5196794 Msa1136550:CDS 40.0%
TTGCTGCTGAAATGGTAAAG+AGG + chr7_4:5196868-5196887 Msa1136550:CDS 40.0%
!! GTCCAAGTGTTGTTGATTTG+GGG + chr7_4:5197000-5197019 Msa1136550:CDS 40.0%
TGTTGGAATTGTTGGGCTTT+GGG + chr7_4:5197025-5197044 Msa1136550:CDS 40.0%
!!! GATTTTTCGAGGAAACGTGA+GGG - chr7_4:5197123-5197142 None:intergenic 40.0%
!!! TGATTTTTCGAGGAAACGTG+AGG - chr7_4:5197124-5197143 None:intergenic 40.0%
GAGAACATGAGTGTCAATAC+CGG - chr7_4:5197250-5197269 None:intergenic 40.0%
! GCGACTACATCTAGACTTTT+CGG + chr7_4:5197270-5197289 Msa1136550:three_prime_UTR 40.0%
GGCACTAGTATTAACCCTTA+GGG - chr7_4:5197397-5197416 None:intergenic 40.0%
AGTTCCTTCACCAAGATCAG+AGG + chr7_4:5195493-5195512 Msa1136550:CDS 45.0%
TAGAGATCCACCTCTGATCT+TGG - chr7_4:5195506-5195525 None:intergenic 45.0%
AACCAACCCTTAACACCGAT+TGG + chr7_4:5196379-5196398 Msa1136550:CDS 45.0%
!! AGATCGCCAATCGGTGTTAA+GGG - chr7_4:5196388-5196407 None:intergenic 45.0%
!! AAGATCGCCAATCGGTGTTA+AGG - chr7_4:5196389-5196408 None:intergenic 45.0%
ATCTTTCCGTGCAAAACTGG+TGG + chr7_4:5196404-5196423 Msa1136550:CDS 45.0%
!! CCGGATTAGTCACTGAAGAA+AGG - chr7_4:5196458-5196477 None:intergenic 45.0%
CATCCACCACTAATCACCAT+TGG + chr7_4:5196492-5196511 Msa1136550:CDS 45.0%
TCACCAATGGTGATTAGTGG+TGG - chr7_4:5196498-5196517 None:intergenic 45.0%
GTAATCCATGAGCCTGAAAG+AGG + chr7_4:5196531-5196550 Msa1136550:CDS 45.0%
AACAACCTCTTTCAGGCTCA+TGG - chr7_4:5196539-5196558 None:intergenic 45.0%
! TTTGGGGGACCTTTAGAAGA+AGG + chr7_4:5196744-5196763 Msa1136550:CDS 45.0%
! TTGGGGGACCTTTAGAAGAA+GGG + chr7_4:5196745-5196764 Msa1136550:CDS 45.0%
GGTGAGTCCTAAAGATGATG+GGG + chr7_4:5196776-5196795 Msa1136550:CDS 45.0%
!! AGTCCTAAAGATGATGGGGT+GGG + chr7_4:5196780-5196799 Msa1136550:CDS 45.0%
TTTCCCACCCCATCATCTTT+AGG - chr7_4:5196786-5196805 None:intergenic 45.0%
GGTAAAGAGGAATGTGGTTG+AGG + chr7_4:5196881-5196900 Msa1136550:CDS 45.0%
AAGAGGAATGTGGTTGAGGT+TGG + chr7_4:5196885-5196904 Msa1136550:CDS 45.0%
! TCTTTGATGGGTATTGTGGG+TGG + chr7_4:5196919-5196938 Msa1136550:CDS 45.0%
! CTTTGATGGGTATTGTGGGT+GGG + chr7_4:5196920-5196939 Msa1136550:CDS 45.0%
CTCCCCAAATCAACAACACT+TGG - chr7_4:5197005-5197024 None:intergenic 45.0%
!! GTTGTTGATTTGGGGAGTGT+TGG + chr7_4:5197008-5197027 Msa1136550:CDS 45.0%
!! TTGGGGAGTGTTGGAATTGT+TGG + chr7_4:5197017-5197036 Msa1136550:CDS 45.0%
!! TGGGGAGTGTTGGAATTGTT+GGG + chr7_4:5197018-5197037 Msa1136550:CDS 45.0%
GTGTTGGAATTGTTGGGCTT+TGG + chr7_4:5197024-5197043 Msa1136550:CDS 45.0%
AACGTGAGGGTGGGTAAAAT+CGG - chr7_4:5197110-5197129 None:intergenic 45.0%
!! ATACACATGGAGAGTGCTTG+TGG + chr7_4:5197190-5197209 Msa1136550:three_prime_UTR 45.0%
!! TACACATGGAGAGTGCTTGT+GGG + chr7_4:5197191-5197210 Msa1136550:three_prime_UTR 45.0%
TTGTGGGGATGGGAAAAGTT+GGG + chr7_4:5197207-5197226 Msa1136550:three_prime_UTR 45.0%
! GGCTCTAATTGAAAGGGTAC+CGG + chr7_4:5197228-5197247 Msa1136550:three_prime_UTR 45.0%
GTGTAGTAGCAAGACCCTAA+GGG + chr7_4:5197380-5197399 Msa1136550:three_prime_UTR 45.0%
GGGCACTAGTATTAACCCTT+AGG - chr7_4:5197398-5197417 None:intergenic 45.0%
TTTGGAAGCTATTGGCAAGC+AGG + chr7_4:5197448-5197467 Msa1136550:three_prime_UTR 45.0%
AGCAGATACTTCCATCCATC+AGG - chr7_4:5197619-5197638 None:intergenic 45.0%
TCCTTCACCAAGATCAGAGG+TGG + chr7_4:5195496-5195515 Msa1136550:CDS 50.0%
TCCACCTCTGATCTTGGTGA+AGG - chr7_4:5195500-5195519 None:intergenic 50.0%
TGATGACATGTGGCAACCTG+TGG - chr7_4:5196337-5196356 None:intergenic 50.0%
TGCACGGAAAGATCGCCAAT+CGG - chr7_4:5196397-5196416 None:intergenic 50.0%
GCGATCTTTCCGTGCAAAAC+TGG + chr7_4:5196401-5196420 Msa1136550:CDS 50.0%
GACTAATCCGGACACTGTTG+TGG + chr7_4:5196467-5196486 Msa1136550:CDS 50.0%
GGATGATCCACAACAGTGTC+CGG - chr7_4:5196477-5196496 None:intergenic 50.0%
GTGAGCCCATTTGTCACCAA+TGG - chr7_4:5196511-5196530 None:intergenic 50.0%
! TGATCCCTGATGGGCCTATT+GGG - chr7_4:5196635-5196654 None:intergenic 50.0%
! ATGATCCCTGATGGGCCTAT+TGG - chr7_4:5196636-5196655 None:intergenic 50.0%
!! GAGTCCTAAAGATGATGGGG+TGG + chr7_4:5196779-5196798 Msa1136550:CDS 50.0%
!! GATGATGGGGTGGGAAAAAG+TGG + chr7_4:5196789-5196808 Msa1136550:CDS 50.0%
TGTGGGTCTTGCTGCTGAAA+TGG + chr7_4:5196860-5196879 Msa1136550:CDS 50.0%
GACGAAGTTCTCGGGCTTAT+GGG + chr7_4:5197047-5197066 Msa1136550:CDS 50.0%
TTCTCGGGCTTATGGGCAAA+AGG + chr7_4:5197054-5197073 Msa1136550:CDS 50.0%
GGCAAAAGGAAGGTCAAGTG+AGG + chr7_4:5197068-5197087 Msa1136550:CDS 50.0%
ACGTGAGGGTGGGTAAAATC+GGG - chr7_4:5197109-5197128 None:intergenic 50.0%
TTTCGAGGAAACGTGAGGGT+GGG - chr7_4:5197119-5197138 None:intergenic 50.0%
!! TTTTCGAGGAAACGTGAGGG+TGG - chr7_4:5197120-5197139 None:intergenic 50.0%
!! ACACATGGAGAGTGCTTGTG+GGG + chr7_4:5197192-5197211 Msa1136550:three_prime_UTR 50.0%
CTTGTGGGGATGGGAAAAGT+TGG + chr7_4:5197206-5197225 Msa1136550:three_prime_UTR 50.0%
GGTGTAGTAGCAAGACCCTA+AGG + chr7_4:5197379-5197398 Msa1136550:three_prime_UTR 50.0%
!! CGCCAATCGGTGTTAAGGGT+TGG - chr7_4:5196384-5196403 None:intergenic 55.0%
TTCCGTGCAAAACTGGTGGC+CGG + chr7_4:5196408-5196427 Msa1136550:CDS 55.0%
!! GGGCTTTGGGACGAAGTTCT+CGG + chr7_4:5197038-5197057 Msa1136550:CDS 55.0%
!! GGCTTTGGGACGAAGTTCTC+GGG + chr7_4:5197039-5197058 Msa1136550:CDS 55.0%
GGACGAAGTTCTCGGGCTTA+TGG + chr7_4:5197046-5197065 Msa1136550:CDS 55.0%
CGGGCTTATGGGCAAAAGGA+AGG + chr7_4:5197058-5197077 Msa1136550:CDS 55.0%
!! ATGGAGAGTGCTTGTGGGGA+TGG + chr7_4:5197196-5197215 Msa1136550:three_prime_UTR 55.0%
!! TGGAGAGTGCTTGTGGGGAT+GGG + chr7_4:5197197-5197216 Msa1136550:three_prime_UTR 55.0%
! CTCCGGCCACCAGTTTTGCA+CGG - chr7_4:5196413-5196432 None:intergenic 60.0%
AACGGCCCAATAGGCCCATC+AGG + chr7_4:5196627-5196646 Msa1136550:CDS 60.0%
ACGGCCCAATAGGCCCATCA+GGG + chr7_4:5196628-5196647 Msa1136550:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 5195246 5197806 5195246 ID=Msa1136550;Name=Msa1136550
chr7_4 mRNA 5195246 5197806 5195246 ID=Msa1136550-mRNA-1;Parent=Msa1136550;Name=Msa1136550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=223|1|1|1|0|0|2|718|291
chr7_4 exon 5195246 5195595 5195246 ID=Msa1136550-mRNA-1:exon:1361;Parent=Msa1136550-mRNA-1
chr7_4 exon 5196340 5197806 5196340 ID=Msa1136550-mRNA-1:exon:1362;Parent=Msa1136550-mRNA-1
chr7_4 five_prime_UTR 5195246 5195468 5195246 ID=Msa1136550-mRNA-1:five_prime_utr;Parent=Msa1136550-mRNA-1
chr7_4 CDS 5195469 5195595 5195469 ID=Msa1136550-mRNA-1:cds;Parent=Msa1136550-mRNA-1
chr7_4 CDS 5196340 5197088 5196340 ID=Msa1136550-mRNA-1:cds;Parent=Msa1136550-mRNA-1
chr7_4 three_prime_UTR 5197089 5197806 5197089 ID=Msa1136550-mRNA-1:three_prime_utr;Parent=Msa1136550-mRNA-1
Gene Sequence

>Msa1136550

ATGGATGCTTGTTTTCTCTCTCACAGTTCCTTCACCAAGATCAGAGGTGGATCTCTACCTTACTCCAAAAGATTTTCTCATCAACAATTCAATTCAAGAAAAGTTGGAGTTCCATTTCATGTATCATGTTGCCACATGTCATCATCATCACCATCGGATGATGAAAAACCAACCCTTAACACCGATTGGCGATCTTTCCGTGCAAAACTGGTGGCCGGAGAACAATTACTAAAGCCCGAATTCCTTTCTTCAGTGACTAATCCGGACACTGTTGTGGATCATCCACCACTAATCACCATTGGTGACAAATGGGCTCACGTAATCCATGAGCCTGAAAGAGGTTGTTTATTAATAGCAACAGAAAAGCTTGATGGGGTTCACATTTTCGAACGAACGGTGATCCTTCTTTTATCAAACGGCCCAATAGGCCCATCAGGGATCATACTCAATAGACCATCTTTGATGTCTATCAAAGAAACAAGATCAACTGCATTTGATGTAATGGGTACTTTTTCTAATAGTCCTTTGTATTTTGGGGGACCTTTAGAAGAAGGGTTGTTTTTGGTGAGTCCTAAAGATGATGGGGTGGGAAAAAGTGGTGTTTTTGATGAAGTGATGAAGGGTTTGTATTATGGTACTAAAGAAAGTGTGGGTCTTGCTGCTGAAATGGTAAAGAGGAATGTGGTTGAGGTTGGTGATTTTAGATTCTTTGATGGGTATTGTGGGTGGGAGAAAGAACAATTGAGAGATGAAATTAGGGATGGTTATTGGACTGTCGCTGCTTGTAGTCCAAGTGTTGTTGATTTGGGGAGTGTTGGAATTGTTGGGCTTTGGGACGAAGTTCTCGGGCTTATGGGCAAAAGGAAGGTCAAGTGA
Protein sequence

>Msa1136550

MDACFLSHSSFTKIRGGSLPYSKRFSHQQFNSRKVGVPFHVSCCHMSSSSPSDDEKPTLNTDWRSFRAKLVAGEQLLKPEFLSSVTNPDTVVDHPPLITIGDKWAHVIHEPERGCLLIATEKLDGVHIFERTVILLLSNGPIGPSGIILNRPSLMSIKETRSTAFDVMGTFSNSPLYFGGPLEEGLFLVSPKDDGVGKSGVFDEVMKGLYYGTKESVGLAAEMVKRNVVEVGDFRFFDGYCGWEKEQLRDEIRDGYWTVAACSPSVVDLGSVGIVGLWDEVLGLMGKRKVK*