AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0040660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040660 MtrunA17_Chr1g0205951 93.970 199 12 0 1 199 1 199 9.84e-137 380
Msa0040660 MtrunA17_Chr5g0394151 63.902 205 64 2 1 199 1 201 6.91e-96 277
Msa0040660 MtrunA17_Chr1g0191761 36.943 157 96 3 20 173 26 182 1.83e-31 113
Msa0040660 MtrunA17_Chr7g0272941 38.889 144 84 3 19 159 17 159 5.32e-31 112
Msa0040660 MtrunA17_Chr1g0191781 32.727 165 105 3 11 169 6 170 2.51e-27 102
Msa0040660 MtrunA17_Chr1g0191771 34.328 134 87 1 31 163 46 179 3.42e-23 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040660 AT3G06035.1 58.101 179 71 2 25 199 21 199 1.52e-71 216
Msa0040660 AT5G19250.1 61.078 167 63 1 24 188 19 185 5.31e-68 206
Msa0040660 AT1G54860.1 41.808 177 85 4 1 168 2 169 3.09e-46 151
Msa0040660 AT5G19240.1 44.767 172 89 3 23 192 20 187 9.69e-46 150
Msa0040660 AT5G19230.2 44.970 169 81 3 1 169 1 157 2.21e-40 136
Msa0040660 AT5G19230.1 44.970 169 81 3 1 169 1 157 2.21e-40 136

Find 42 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGACATTTAACTAAA+AGG 0.202634 1_1:+70940170 None:intergenic
CCAAGTTCTCTCTATTCTTC+TGG 0.209568 1_1:-70940992 Msa0040660:CDS
GTTATGCCTGCCTGTGTTCC+TGG 0.264326 1_1:-70940121 Msa0040660:CDS
TCCAACTTTGGAAATCAAAT+TGG 0.265283 1_1:+70939928 None:intergenic
TGAGAGAGATAATCTTTATC+AGG 0.299934 1_1:-70940362 Msa0040660:CDS
TGACATTTAACTAAAAGGTT+TGG 0.309878 1_1:+70940175 None:intergenic
AACTAAAAGGTTTGGGTAGT+TGG 0.309926 1_1:+70940183 None:intergenic
GTATTGGCTCAGAAGATAAC+TGG 0.333128 1_1:-70940015 Msa0040660:CDS
GACATTTAACTAAAAGGTTT+GGG 0.347354 1_1:+70940176 None:intergenic
CCAGAAGAATAGAGAGAACT+TGG 0.347419 1_1:+70940992 None:intergenic
GCAATAAATCATTCCAACTT+TGG 0.358521 1_1:+70939916 None:intergenic
TACATGGTTGATTCTTGAAT+TGG 0.360348 1_1:+70940254 None:intergenic
ATTGGCAGCATTAGAACTAT+AGG 0.374058 1_1:+70939946 None:intergenic
GCCAATTTGATTTCCAAAGT+TGG 0.375408 1_1:-70939929 Msa0040660:CDS
TTGGCAGCATTAGAACTATA+GGG 0.380106 1_1:+70939947 None:intergenic
CATCCTCTAGGTATCATAAC+AGG 0.398840 1_1:+70939864 None:intergenic
TCACCGTCATCACATTTAAC+TGG 0.400596 1_1:+70940930 None:intergenic
GACACACAGTATACAGGAAT+TGG 0.421140 1_1:-70940037 Msa0040660:CDS
TGCTCAATTCTCTTGTTAGC+TGG 0.427277 1_1:-70939896 Msa0040660:CDS
CTCAACGACACACAGTATAC+AGG 0.481619 1_1:-70940043 Msa0040660:CDS
TTGACCACTGACACTCCAAC+AGG 0.483770 1_1:-70939980 Msa0040660:CDS
TTTGGGTAGTTGGAGAATTG+AGG 0.485525 1_1:+70940193 None:intergenic
ATTGGCTATTTCTGATACAA+AGG 0.497292 1_1:-70940152 Msa0040660:CDS
ACGAGGCTTGAAGCTAGGCC+AGG 0.504069 1_1:+70940103 None:intergenic
TGAAGCTAGGCCAGGAACAC+AGG 0.509872 1_1:+70940111 None:intergenic
GCTAGGCCAGGAACACAGGC+AGG 0.513127 1_1:+70940115 None:intergenic
GAGAATTGAGGTTCAGTACC+TGG 0.522201 1_1:+70940205 None:intergenic
GCTATTTCTGATACAAAGGA+TGG 0.531181 1_1:-70940148 Msa0040660:CDS
CAGTATACAGGAATTGGTAT+TGG 0.536270 1_1:-70940031 Msa0040660:CDS
GCACCTGTGGTATTTGTACA+TGG 0.542966 1_1:+70940238 None:intergenic
TCGTGAAGTTAGTGAGGACG+AGG 0.562843 1_1:+70940086 None:intergenic
TGAGGACGAGGCTTGAAGCT+AGG 0.566605 1_1:+70940098 None:intergenic
GTTTCCTGTTGGAGTGTCAG+TGG 0.576612 1_1:+70939976 None:intergenic
GAGATTTCGTGAAGTTAGTG+AGG 0.582648 1_1:+70940080 None:intergenic
GAGAGAGATAATCTTTATCA+GGG 0.592178 1_1:-70940361 Msa0040660:CDS
TATCCAGTTAAATGTGATGA+CGG 0.600716 1_1:-70940933 Msa0040660:intron
TGGTACTGTATTAGCACCTG+TGG 0.607702 1_1:+70940225 None:intergenic
TGGCAGCATTAGAACTATAG+GGG 0.612217 1_1:+70939948 None:intergenic
ACAGGTGCTAATACAGTACC+AGG 0.621692 1_1:-70940223 Msa0040660:CDS
CAGAAGAATAGAGAGAACTT+GGG 0.624874 1_1:+70940993 None:intergenic
CAACCATGTACAAATACCAC+AGG 0.631778 1_1:-70940241 Msa0040660:CDS
GGCAGCATTAGAACTATAGG+GGG 0.666389 1_1:+70939949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGACCAATACAAAATA+TGG + chr1_1:70940030-70940049 None:intergenic 20.0%
!!! ATTTTGACCAATACAAAATA+TGG + chr1_1:70940030-70940049 None:intergenic 20.0%
!! TCTTAGACCATATTTTGTAT+TGG - chr1_1:70940020-70940039 Msa0040660:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr1_1:70940095-70940114 Msa0040660:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr1_1:70940095-70940114 Msa0040660:CDS 25.0%
! GACATTTAACTAAAAGGTTT+GGG + chr1_1:70940706-70940725 None:intergenic 25.0%
! TGACATTTAACTAAAAGGTT+TGG + chr1_1:70940707-70940726 None:intergenic 25.0%
! CCAATTGACATTTAACTAAA+AGG + chr1_1:70940712-70940731 None:intergenic 25.0%
!!! CCTTTTAGTTAAATGTCAAT+TGG - chr1_1:70940709-70940728 Msa0040660:intron 25.0%
!! TCTTAGACCATATTTTGTAT+TGG - chr1_1:70940020-70940039 Msa0040660:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr1_1:70940095-70940114 Msa0040660:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr1_1:70940095-70940114 Msa0040660:CDS 25.0%
! GACATTTAACTAAAAGGTTT+GGG + chr1_1:70940706-70940725 None:intergenic 25.0%
! TGACATTTAACTAAAAGGTT+TGG + chr1_1:70940707-70940726 None:intergenic 25.0%
! CCAATTGACATTTAACTAAA+AGG + chr1_1:70940712-70940731 None:intergenic 25.0%
!!! CCTTTTAGTTAAATGTCAAT+TGG - chr1_1:70940709-70940728 Msa0040660:intron 25.0%
TATCCAGTTAAATGTGATGA+CGG - chr1_1:70939946-70939965 Msa0040660:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG + chr1_1:70940389-70940408 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG + chr1_1:70940455-70940474 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG + chr1_1:70940495-70940514 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG - chr1_1:70940517-70940536 Msa0040660:intron 30.0%
GAGAGAGATAATCTTTATCA+GGG - chr1_1:70940518-70940537 Msa0040660:intron 30.0%
! TACATGGTTGATTCTTGAAT+TGG + chr1_1:70940628-70940647 None:intergenic 30.0%
!! ATTGGCTATTTCTGATACAA+AGG - chr1_1:70940727-70940746 Msa0040660:intron 30.0%
! TCCAACTTTGGAAATCAAAT+TGG + chr1_1:70940954-70940973 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG + chr1_1:70940966-70940985 None:intergenic 30.0%
TATCCAGTTAAATGTGATGA+CGG - chr1_1:70939946-70939965 Msa0040660:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG + chr1_1:70940389-70940408 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG + chr1_1:70940455-70940474 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG + chr1_1:70940495-70940514 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG - chr1_1:70940517-70940536 Msa0040660:intron 30.0%
GAGAGAGATAATCTTTATCA+GGG - chr1_1:70940518-70940537 Msa0040660:intron 30.0%
! TACATGGTTGATTCTTGAAT+TGG + chr1_1:70940628-70940647 None:intergenic 30.0%
!! ATTGGCTATTTCTGATACAA+AGG - chr1_1:70940727-70940746 Msa0040660:intron 30.0%
! TCCAACTTTGGAAATCAAAT+TGG + chr1_1:70940954-70940973 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG + chr1_1:70940966-70940985 None:intergenic 30.0%
CAGAAGAATAGAGAGAACTT+GGG + chr1_1:70939889-70939908 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG + chr1_1:70940168-70940187 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG - chr1_1:70940288-70940307 Msa0040660:CDS 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG + chr1_1:70940294-70940313 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG + chr1_1:70940496-70940515 None:intergenic 35.0%
AACTAAAAGGTTTGGGTAGT+TGG + chr1_1:70940699-70940718 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG - chr1_1:70940731-70940750 Msa0040660:intron 35.0%
CAGTATACAGGAATTGGTAT+TGG - chr1_1:70940848-70940867 Msa0040660:intron 35.0%
TTGGCAGCATTAGAACTATA+GGG + chr1_1:70940935-70940954 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG + chr1_1:70940936-70940955 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG - chr1_1:70940950-70940969 Msa0040660:CDS 35.0%
CAGAAGAATAGAGAGAACTT+GGG + chr1_1:70939889-70939908 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG + chr1_1:70940168-70940187 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG - chr1_1:70940288-70940307 Msa0040660:CDS 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG + chr1_1:70940294-70940313 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG + chr1_1:70940496-70940515 None:intergenic 35.0%
AACTAAAAGGTTTGGGTAGT+TGG + chr1_1:70940699-70940718 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG - chr1_1:70940731-70940750 Msa0040660:intron 35.0%
CAGTATACAGGAATTGGTAT+TGG - chr1_1:70940848-70940867 Msa0040660:intron 35.0%
TTGGCAGCATTAGAACTATA+GGG + chr1_1:70940935-70940954 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG + chr1_1:70940936-70940955 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG - chr1_1:70940950-70940969 Msa0040660:CDS 35.0%
CCAGAAGAATAGAGAGAACT+TGG + chr1_1:70939890-70939909 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG - chr1_1:70939887-70939906 Msa0040660:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG + chr1_1:70939952-70939971 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG + chr1_1:70940169-70940188 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG + chr1_1:70940319-70940338 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG - chr1_1:70940410-70940429 Msa0040660:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG + chr1_1:70940478-70940497 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG + chr1_1:70940607-70940626 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG - chr1_1:70940638-70940657 Msa0040660:intron 40.0%
TTTGGGTAGTTGGAGAATTG+AGG + chr1_1:70940689-70940708 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG + chr1_1:70940802-70940821 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG - chr1_1:70940842-70940861 Msa0040660:intron 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG - chr1_1:70940864-70940883 Msa0040660:intron 40.0%
TGGCAGCATTAGAACTATAG+GGG + chr1_1:70940934-70940953 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG - chr1_1:70940983-70941002 Msa0040660:CDS 40.0%
CCAGAAGAATAGAGAGAACT+TGG + chr1_1:70939890-70939909 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG - chr1_1:70939887-70939906 Msa0040660:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG + chr1_1:70939952-70939971 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG + chr1_1:70940169-70940188 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG + chr1_1:70940319-70940338 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG - chr1_1:70940410-70940429 Msa0040660:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG + chr1_1:70940478-70940497 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG + chr1_1:70940607-70940626 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG - chr1_1:70940638-70940657 Msa0040660:intron 40.0%
TTTGGGTAGTTGGAGAATTG+AGG + chr1_1:70940689-70940708 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG + chr1_1:70940802-70940821 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG - chr1_1:70940842-70940861 Msa0040660:intron 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG - chr1_1:70940864-70940883 Msa0040660:intron 40.0%
TGGCAGCATTAGAACTATAG+GGG + chr1_1:70940934-70940953 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG - chr1_1:70940983-70941002 Msa0040660:CDS 40.0%
TGTGCATGTGTGCCAGAAAA+AGG + chr1_1:70940425-70940444 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG + chr1_1:70940644-70940663 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG + chr1_1:70940657-70940676 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG - chr1_1:70940656-70940675 Msa0040660:intron 45.0%
GAGAATTGAGGTTCAGTACC+TGG + chr1_1:70940677-70940696 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG - chr1_1:70940836-70940855 Msa0040660:intron 45.0%
GGCAGCATTAGAACTATAGG+GGG + chr1_1:70940933-70940952 None:intergenic 45.0%
TGTGCATGTGTGCCAGAAAA+AGG + chr1_1:70940425-70940444 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG + chr1_1:70940644-70940663 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG + chr1_1:70940657-70940676 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG - chr1_1:70940656-70940675 Msa0040660:intron 45.0%
GAGAATTGAGGTTCAGTACC+TGG + chr1_1:70940677-70940696 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG - chr1_1:70940836-70940855 Msa0040660:intron 45.0%
GGCAGCATTAGAACTATAGG+GGG + chr1_1:70940933-70940952 None:intergenic 45.0%
TCATCGCAGTCGCGAGATTA+GGG + chr1_1:70940242-70940261 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG + chr1_1:70940243-70940262 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG + chr1_1:70940796-70940815 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG - chr1_1:70940899-70940918 Msa0040660:intron 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG + chr1_1:70940906-70940925 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG + chr1_1:70940917-70940936 None:intergenic 50.0%
TCATCGCAGTCGCGAGATTA+GGG + chr1_1:70940242-70940261 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG + chr1_1:70940243-70940262 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG + chr1_1:70940796-70940815 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG - chr1_1:70940899-70940918 Msa0040660:intron 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG + chr1_1:70940906-70940925 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG + chr1_1:70940917-70940936 None:intergenic 50.0%
GTTATGCCTGCCTGTGTTCC+TGG - chr1_1:70940758-70940777 Msa0040660:intron 55.0%
TGAAGCTAGGCCAGGAACAC+AGG + chr1_1:70940771-70940790 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG + chr1_1:70940784-70940803 None:intergenic 55.0%
GTTATGCCTGCCTGTGTTCC+TGG - chr1_1:70940758-70940777 Msa0040660:intron 55.0%
TGAAGCTAGGCCAGGAACAC+AGG + chr1_1:70940771-70940790 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG + chr1_1:70940784-70940803 None:intergenic 55.0%
ACGAGGCTTGAAGCTAGGCC+AGG + chr1_1:70940779-70940798 None:intergenic 60.0%
ACGAGGCTTGAAGCTAGGCC+AGG + chr1_1:70940779-70940798 None:intergenic 60.0%
GCTAGGCCAGGAACACAGGC+AGG + chr1_1:70940767-70940786 None:intergenic 65.0%
GCTAGGCCAGGAACACAGGC+AGG + chr1_1:70940767-70940786 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 70939877 70941024 70939877 ID=Msa0040660;Name=Msa0040660
chr1_1 mRNA 70939877 70941024 70939877 ID=Msa0040660-mRNA-1;Parent=Msa0040660;Name=Msa0040660-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|199
chr1_1 exon 70940934 70941024 70940934 ID=Msa0040660-mRNA-1:exon:23441;Parent=Msa0040660-mRNA-1
chr1_1 exon 70939877 70940385 70939877 ID=Msa0040660-mRNA-1:exon:23440;Parent=Msa0040660-mRNA-1
chr1_1 CDS 70940934 70941024 70940934 ID=Msa0040660-mRNA-1:cds;Parent=Msa0040660-mRNA-1
chr1_1 CDS 70939877 70940385 70939877 ID=Msa0040660-mRNA-1:cds;Parent=Msa0040660-mRNA-1
Gene Sequence

>Msa0040660

ATGGTGTTGCCCAAGTTCTCTCTATTCTTCTGGATTCTTTCTTCTTCATGTGTTATTCTCTTGATGAAATATCCAGTTAAATGTGATGACGATGAGAGAGATAATCTTTATCAGGGTATCAACAAGTATCGAGCATCATTAAACTTGAAATCTCTCACAAAAAATAAAAATGCAGATTGCCTTGCTGATAAAATAGCTGACCAATTCAAGAATCAACCATGTACAAATACCACAGGTGCTAATACAGTACCAGGTACTGAACCTCAATTCTCCAACTACCCAAACCTTTTAGTTAAATGTCAATTGGCTATTTCTGATACAAAGGATGGAGCTGTTATGCCTGCCTGTGTTCCTGGCCTAGCTTCAAGCCTCGTCCTCACTAACTTCACGAAATCTCTCTACTCACAGAATCTCAACGACACACAGTATACAGGAATTGGTATTGGCTCAGAAGATAACTGGATTGTCGTTGTCTTGACCACTGACACTCCAACAGGAAACTTTGCCCCCTATAGTTCTAATGCTGCCAATTTGATTTCCAAAGTTGGAATGATTTATTGCTCAATTCTCTTGTTAGCTGGCAAAATTTTCCTGTTATGA

Protein sequence

>Msa0040660

MVLPKFSLFFWILSSSCVILLMKYPVKCDDDERDNLYQGINKYRASLNLKSLTKNKNADCLADKIADQFKNQPCTNTTGANTVPGTEPQFSNYPNLLVKCQLAISDTKDGAVMPACVPGLASSLVLTNFTKSLYSQNLNDTQYTGIGIGSEDNWIVVVLTTDTPTGNFAPYSSNAANLISKVGMIYCSILLLAGKIFLL*