AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1173160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173160 MtrunA17_Chr1g0152301 97.516 161 4 0 1 161 1 161 4.23e-115 322
Msa1173160 MtrunA17_Chr8g0372611 93.789 161 9 1 1 161 1 160 1.49e-98 280
Msa1173160 MtrunA17_Chr4g0065131 93.168 161 10 1 1 161 1 160 2.92e-98 280
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173160 AT5G23740.1 87.578 161 18 1 1 161 1 159 9.39e-93 266
Msa1173160 AT3G48930.1 86.335 161 21 1 1 161 1 160 3.66e-91 262
Msa1173160 AT4G30800.1 86.335 161 20 1 1 161 1 159 8.33e-89 256

Find 58 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCACGATCTCATCAAATT+TGG 0.236368 8_1:-3382022 Msa1173160:three_prime_UTR
GAAGGGGATCATGTTATTAT+TGG 0.267190 8_1:-3382341 Msa1173160:CDS
CGTTTACAGGAGTCTGAGTT+TGG 0.301109 8_1:-3382107 Msa1173160:exon
ATAATTCCTCCTAACAATAA+TGG 0.308146 8_1:+3382543 None:intergenic
AAGCGATTGCCACCCTTTCC+AGG 0.318925 8_1:+3383151 None:intergenic
TAAGCACTGCACTGCCATTA+TGG 0.327802 8_1:+3381906 None:intergenic
TGAATAAAACCATTATTGTT+AGG 0.334061 8_1:-3382552 Msa1173160:CDS
GACTCCTGTAAACGCCTTCT+TGG 0.362038 8_1:+3382116 None:intergenic
GGTGCCAAGAAGGCGTTTAC+AGG 0.366657 8_1:-3382120 Msa1173160:CDS
GGGACACTTCTTGTCAATAT+AGG 0.373630 8_1:+3382636 None:intergenic
TTGCTTCAGAAAAGCCTTCT+CGG 0.396604 8_1:+3383219 None:intergenic
TTTGAGAAAGAAACCTTCAA+TGG 0.449940 8_1:+3383087 None:intergenic
CAGTAAAACATTTGAAACTA+AGG 0.459973 8_1:+3382054 None:intergenic
TACCTACTGCGGCAGATTCA+AGG 0.460205 8_1:-3383399 None:intergenic
CCCTAGGAGTCTTAAAACTA+AGG 0.476406 8_1:+3383113 None:intergenic
ACTCCTAGGGAAGCCATTGA+AGG 0.484194 8_1:-3383100 Msa1173160:intron
GCTGGATCTTCTAGTGGTGC+CGG 0.487132 8_1:-3382141 Msa1173160:CDS
ATGTTATTATTGGCCAATGC+AGG 0.499585 8_1:-3382331 Msa1173160:intron
TACAATGATCTAACCTGCAT+TGG 0.500383 8_1:+3382318 None:intergenic
ATCCGAGGTCGTATCATATC+CGG 0.504599 8_1:-3382595 Msa1173160:CDS
TCTGGGAAGGGGAAGAGACC+TGG 0.510724 8_1:-3383169 Msa1173160:CDS
AACCTCACTGTCTTAGAAAG+TGG 0.510906 8_1:+3382186 None:intergenic
TAGTGGTGCCGGTGCCAAGA+AGG 0.518324 8_1:-3382130 Msa1173160:CDS
CAGATTCAAGGTGCAAAACT+CGG 0.518925 8_1:-3383387 Msa1173160:five_prime_UTR
GACAAGAAGTGTCCCTTCAC+TGG 0.524673 8_1:-3382628 Msa1173160:CDS
ATCACCTTGTTTCCGAGTGA+AGG 0.529932 8_1:-3382363 Msa1173160:CDS
AAGGGGAAGAGACCTGGAAA+GGG 0.530695 8_1:-3383163 Msa1173160:CDS
CCTTGTTTCCGAGTGAAGGA+AGG 0.534218 8_1:-3382359 Msa1173160:CDS
CCTTCCTTCACTCGGAAACA+AGG 0.542963 8_1:+3382359 None:intergenic
TGCTCGAAGATATCTGGGAA+GGG 0.550883 8_1:-3383181 Msa1173160:CDS
CTTGTTTCCGAGTGAAGGAA+GGG 0.551830 8_1:-3382358 Msa1173160:CDS
ATAAAACCATTATTGTTAGG+AGG 0.551945 8_1:-3382549 Msa1173160:CDS
GTATTGAAAGTCATCCCAGC+TGG 0.559579 8_1:-3382159 Msa1173160:CDS
GGGAAGAGACCTGGAAAGGG+TGG 0.564985 8_1:-3383160 Msa1173160:CDS
TTTGAATTTGTAGACCGAGA+AGG 0.567346 8_1:-3383233 Msa1173160:intron
GCACCACTAGAAGATCCAGC+TGG 0.568341 8_1:+3382144 None:intergenic
CAAACTAACTTGTTCAGCCA+TGG 0.568984 8_1:+3383332 None:intergenic
ATCCCAGCTGGATCTTCTAG+TGG 0.570183 8_1:-3382147 Msa1173160:CDS
GAAGGGGAAGAGACCTGGAA+AGG 0.571357 8_1:-3383164 Msa1173160:CDS
CGTGTGCAGGAATATTAGAA+TGG 0.572181 8_1:+3382387 None:intergenic
CTTAGCACTATGGCAAGTAC+CGG 0.576116 8_1:+3382576 None:intergenic
CATGATCCCCTTCCTTCACT+CGG 0.580378 8_1:+3382351 None:intergenic
CTAACTTGTTCAGCCATGGT+TGG 0.581084 8_1:+3383336 None:intergenic
GTGCTCGAAGATATCTGGGA+AGG 0.582495 8_1:-3383182 Msa1173160:CDS
AAACCTTCAATGGCTTCCCT+AGG 0.582868 8_1:+3383097 None:intergenic
TTCATCAACTCAACCAACCA+TGG 0.588651 8_1:-3383349 Msa1173160:exon
AAACAAGGTGATACGTGTGC+AGG 0.596227 8_1:+3382374 None:intergenic
GCTCGAAGATATCTGGGAAG+GGG 0.598893 8_1:-3383180 Msa1173160:CDS
GATTGAGACATTACCAGTGA+AGG 0.618985 8_1:+3382615 None:intergenic
TTGTTTCCGAGTGAAGGAAG+GGG 0.622320 8_1:-3382357 Msa1173160:CDS
ATTGAGACATTACCAGTGAA+GGG 0.624989 8_1:+3382616 None:intergenic
CACCACTAGAAGATCCAGCT+GGG 0.629304 8_1:+3382145 None:intergenic
ACTGGTAATGTCTCAATCCG+AGG 0.640857 8_1:-3382610 Msa1173160:CDS
TGTAAACGCCTTCTTGGCAC+CGG 0.648607 8_1:+3382122 None:intergenic
ATTTCATTAAGAAATATCAG+AGG 0.666848 8_1:-3382516 Msa1173160:intron
GTTCGAGTACTAGTGTACAA+TGG 0.690452 8_1:-3381833 Msa1173160:three_prime_UTR
GGCCACTTTCTAAGACAGTG+AGG 0.709841 8_1:-3382188 Msa1173160:CDS
TACCGGATATGATACGACCT+CGG 0.712271 8_1:+3382593 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTATAGATTAATGTGA+AGG + chr8_1:3381861-3381880 None:intergenic 15.0%
!!! TTTTTTGTTTTGATAAATCT+TGG - chr8_1:3381883-3381902 Msa1173160:three_prime_UTR 15.0%
!!! ATTTTTATTTGAAAGTTGTT+CGG - chr8_1:3383389-3383408 Msa1173160:five_prime_UTR 15.0%
!! TAAATTATAGATTAATGTGA+AGG + chr8_1:3381861-3381880 None:intergenic 15.0%
!!! TTTTTTGTTTTGATAAATCT+TGG - chr8_1:3381883-3381902 Msa1173160:three_prime_UTR 15.0%
!!! TAGATTGGTTTATTATTGAT+AGG - chr8_1:3382192-3382211 Msa1173160:intron 20.0%
!! GTTACCAATTTATTTACATA+AGG - chr8_1:3382459-3382478 Msa1173160:intron 20.0%
!! GTATTAGATTACAAAAAGAA+AGG + chr8_1:3382486-3382505 None:intergenic 20.0%
!!! TACCTTTTTCTTAATCTTAT+AGG - chr8_1:3382503-3382522 Msa1173160:intron 20.0%
!! TTCCTATAAGATTAAGAAAA+AGG + chr8_1:3382508-3382527 None:intergenic 20.0%
!! TGAATAAAACCATTATTGTT+AGG - chr8_1:3382612-3382631 Msa1173160:CDS 20.0%
!! ATTTCATTAAGAAATATCAG+AGG - chr8_1:3382648-3382667 Msa1173160:intron 20.0%
!!! TTTTTATTTGAAAGTTGTTC+GGG - chr8_1:3383390-3383409 Msa1173160:five_prime_UTR 20.0%
!!! TAGATTGGTTTATTATTGAT+AGG - chr8_1:3382192-3382211 Msa1173160:intron 20.0%
!! GTTACCAATTTATTTACATA+AGG - chr8_1:3382459-3382478 Msa1173160:intron 20.0%
!! GTATTAGATTACAAAAAGAA+AGG + chr8_1:3382486-3382505 None:intergenic 20.0%
!!! TACCTTTTTCTTAATCTTAT+AGG - chr8_1:3382503-3382522 Msa1173160:intron 20.0%
!! TTCCTATAAGATTAAGAAAA+AGG + chr8_1:3382508-3382527 None:intergenic 20.0%
!! TGAATAAAACCATTATTGTT+AGG - chr8_1:3382612-3382631 Msa1173160:CDS 20.0%
!! ATTTCATTAAGAAATATCAG+AGG - chr8_1:3382648-3382667 Msa1173160:intron 20.0%
! TCTTGGATTTACTAACTAAT+TGG - chr8_1:3381900-3381919 Msa1173160:three_prime_UTR 25.0%
!!! AATCGCTTTTATAAATCTGT+TGG - chr8_1:3382028-3382047 Msa1173160:three_prime_UTR 25.0%
! TAAACCACGAATATGAAAAT+TGG + chr8_1:3382109-3382128 None:intergenic 25.0%
!! TTTTCATATTCGTGGTTTAA+TGG - chr8_1:3382110-3382129 Msa1173160:exon 25.0%
!!! CATTGTGTTTTAAGAATCAT+AGG - chr8_1:3382169-3382188 Msa1173160:CDS 25.0%
! TTTAAGAATCATAGGTAGAT+TGG - chr8_1:3382177-3382196 Msa1173160:CDS 25.0%
! AAGCTTATGAAAATAGCTTA+TGG + chr8_1:3382300-3382319 None:intergenic 25.0%
! AGGACTTATTAAAACAGTTT+AGG + chr8_1:3382370-3382389 None:intergenic 25.0%
! AGGACCTTATGTAAATAAAT+TGG + chr8_1:3382466-3382485 None:intergenic 25.0%
!! TTTTATTCATCTTAGCACTA+TGG + chr8_1:3382601-3382620 None:intergenic 25.0%
! ATAAAACCATTATTGTTAGG+AGG - chr8_1:3382615-3382634 Msa1173160:CDS 25.0%
! ATAATTCCTCCTAACAATAA+TGG + chr8_1:3382624-3382643 None:intergenic 25.0%
!!! TTGTTTTGTTTATCCAAGTA+AGG - chr8_1:3382900-3382919 Msa1173160:intron 25.0%
!! CAGTAAAACATTTGAAACTA+AGG + chr8_1:3383113-3383132 None:intergenic 25.0%
!!! TTTTATTTGAAAGTTGTTCG+GGG - chr8_1:3383391-3383410 Msa1173160:five_prime_UTR 25.0%
! TCTTGGATTTACTAACTAAT+TGG - chr8_1:3381900-3381919 Msa1173160:three_prime_UTR 25.0%
!!! AATCGCTTTTATAAATCTGT+TGG - chr8_1:3382028-3382047 Msa1173160:three_prime_UTR 25.0%
! TAAACCACGAATATGAAAAT+TGG + chr8_1:3382109-3382128 None:intergenic 25.0%
!! TTTTCATATTCGTGGTTTAA+TGG - chr8_1:3382110-3382129 Msa1173160:exon 25.0%
!!! CATTGTGTTTTAAGAATCAT+AGG - chr8_1:3382169-3382188 Msa1173160:CDS 25.0%
! TTTAAGAATCATAGGTAGAT+TGG - chr8_1:3382177-3382196 Msa1173160:CDS 25.0%
! AAGCTTATGAAAATAGCTTA+TGG + chr8_1:3382300-3382319 None:intergenic 25.0%
! AGGACTTATTAAAACAGTTT+AGG + chr8_1:3382370-3382389 None:intergenic 25.0%
! AGGACCTTATGTAAATAAAT+TGG + chr8_1:3382466-3382485 None:intergenic 25.0%
!! TTTTATTCATCTTAGCACTA+TGG + chr8_1:3382601-3382620 None:intergenic 25.0%
! ATAAAACCATTATTGTTAGG+AGG - chr8_1:3382615-3382634 Msa1173160:CDS 25.0%
! ATAATTCCTCCTAACAATAA+TGG + chr8_1:3382624-3382643 None:intergenic 25.0%
!!! TTGTTTTGTTTATCCAAGTA+AGG - chr8_1:3382900-3382919 Msa1173160:intron 25.0%
!! CAGTAAAACATTTGAAACTA+AGG + chr8_1:3383113-3383132 None:intergenic 25.0%
TTTGAGAAAGAAACCTTCAA+TGG + chr8_1:3382080-3382099 None:intergenic 30.0%
! GTTTCCAATTTTCATATTCG+TGG - chr8_1:3382102-3382121 Msa1173160:exon 30.0%
CTCTGTTTGGTAAAAAATAG+TGG + chr8_1:3382277-3382296 None:intergenic 30.0%
TGTGTATGCTTGTTCTTATT+AGG - chr8_1:3382743-3382762 Msa1173160:intron 30.0%
! AATGTTTTACTGTTCTGACT+TGG - chr8_1:3383121-3383140 Msa1173160:CDS 30.0%
! TGCAGTAATTTTTGCCATAA+TGG - chr8_1:3383244-3383263 Msa1173160:intron 30.0%
TTTGAGAAAGAAACCTTCAA+TGG + chr8_1:3382080-3382099 None:intergenic 30.0%
! GTTTCCAATTTTCATATTCG+TGG - chr8_1:3382102-3382121 Msa1173160:exon 30.0%
CTCTGTTTGGTAAAAAATAG+TGG + chr8_1:3382277-3382296 None:intergenic 30.0%
TGTGTATGCTTGTTCTTATT+AGG - chr8_1:3382743-3382762 Msa1173160:intron 30.0%
! AATGTTTTACTGTTCTGACT+TGG - chr8_1:3383121-3383140 Msa1173160:CDS 30.0%
! TGCAGTAATTTTTGCCATAA+TGG - chr8_1:3383244-3383263 Msa1173160:intron 30.0%
TTTGAATTTGTAGACCGAGA+AGG - chr8_1:3381931-3381950 Msa1173160:three_prime_UTR 35.0%
! TTTTTGTGCTCGAAGATATC+TGG - chr8_1:3381977-3381996 Msa1173160:three_prime_UTR 35.0%
! TTTTGTGCTCGAAGATATCT+GGG - chr8_1:3381978-3381997 Msa1173160:three_prime_UTR 35.0%
!!! CCTTAGTTTTAAGACTCCTA+GGG - chr8_1:3382051-3382070 Msa1173160:three_prime_UTR 35.0%
! AAATAGCTTATGGCTCTGTT+TGG + chr8_1:3382290-3382309 None:intergenic 35.0%
AAGCTTTCATGAAGAGCTTA+TGG - chr8_1:3382314-3382333 Msa1173160:intron 35.0%
AGATAAGCTCAAATAAGCCA+TGG - chr8_1:3382427-3382446 Msa1173160:intron 35.0%
! TTGGTAACATAAACATGCCA+TGG + chr8_1:3382447-3382466 None:intergenic 35.0%
ATTGAGACATTACCAGTGAA+GGG + chr8_1:3382551-3382570 None:intergenic 35.0%
TTACTGTATTTGTATGCCAC+AGG - chr8_1:3382704-3382723 Msa1173160:intron 35.0%
ACAAATAGTTAACACACCTG+TGG + chr8_1:3382723-3382742 None:intergenic 35.0%
GAAGGGGATCATGTTATTAT+TGG - chr8_1:3382823-3382842 Msa1173160:intron 35.0%
ATGTTATTATTGGCCAATGC+AGG - chr8_1:3382833-3382852 Msa1173160:intron 35.0%
TACAATGATCTAACCTGCAT+TGG + chr8_1:3382849-3382868 None:intergenic 35.0%
CACAAAAACAGTTCCTTACT+TGG + chr8_1:3382916-3382935 None:intergenic 35.0%
GCAGAAGTAAAGGTAATCAA+GGG + chr8_1:3382947-3382966 None:intergenic 35.0%
AGCAGAAGTAAAGGTAATCA+AGG + chr8_1:3382948-3382967 None:intergenic 35.0%
CTATGAACAAGCAGAAGTAA+AGG + chr8_1:3382957-3382976 None:intergenic 35.0%
CTTTACTTCTGCTTGTTCAT+AGG - chr8_1:3382955-3382974 Msa1173160:intron 35.0%
TTTGAATTTGTAGACCGAGA+AGG - chr8_1:3381931-3381950 Msa1173160:three_prime_UTR 35.0%
! TTTTTGTGCTCGAAGATATC+TGG - chr8_1:3381977-3381996 Msa1173160:three_prime_UTR 35.0%
! TTTTGTGCTCGAAGATATCT+GGG - chr8_1:3381978-3381997 Msa1173160:three_prime_UTR 35.0%
!!! CCTTAGTTTTAAGACTCCTA+GGG - chr8_1:3382051-3382070 Msa1173160:three_prime_UTR 35.0%
! AAATAGCTTATGGCTCTGTT+TGG + chr8_1:3382290-3382309 None:intergenic 35.0%
AAGCTTTCATGAAGAGCTTA+TGG - chr8_1:3382314-3382333 Msa1173160:intron 35.0%
AGATAAGCTCAAATAAGCCA+TGG - chr8_1:3382427-3382446 Msa1173160:intron 35.0%
! TTGGTAACATAAACATGCCA+TGG + chr8_1:3382447-3382466 None:intergenic 35.0%
ATTGAGACATTACCAGTGAA+GGG + chr8_1:3382551-3382570 None:intergenic 35.0%
TTACTGTATTTGTATGCCAC+AGG - chr8_1:3382704-3382723 Msa1173160:intron 35.0%
ACAAATAGTTAACACACCTG+TGG + chr8_1:3382723-3382742 None:intergenic 35.0%
GAAGGGGATCATGTTATTAT+TGG - chr8_1:3382823-3382842 Msa1173160:intron 35.0%
ATGTTATTATTGGCCAATGC+AGG - chr8_1:3382833-3382852 Msa1173160:intron 35.0%
TACAATGATCTAACCTGCAT+TGG + chr8_1:3382849-3382868 None:intergenic 35.0%
CACAAAAACAGTTCCTTACT+TGG + chr8_1:3382916-3382935 None:intergenic 35.0%
GCAGAAGTAAAGGTAATCAA+GGG + chr8_1:3382947-3382966 None:intergenic 35.0%
AGCAGAAGTAAAGGTAATCA+AGG + chr8_1:3382948-3382967 None:intergenic 35.0%
CTATGAACAAGCAGAAGTAA+AGG + chr8_1:3382957-3382976 None:intergenic 35.0%
CTTTACTTCTGCTTGTTCAT+AGG - chr8_1:3382955-3382974 Msa1173160:intron 35.0%
CAGATTCAAGGTGCAAAACT+CGG - chr8_1:3381777-3381796 Msa1173160:three_prime_UTR 40.0%
TTCATCAACTCAACCAACCA+TGG - chr8_1:3381815-3381834 Msa1173160:three_prime_UTR 40.0%
!! CAAACTAACTTGTTCAGCCA+TGG + chr8_1:3381835-3381854 None:intergenic 40.0%
TTGCTTCAGAAAAGCCTTCT+CGG + chr8_1:3381948-3381967 None:intergenic 40.0%
!!! GCCTTAGTTTTAAGACTCCT+AGG - chr8_1:3382050-3382069 Msa1173160:three_prime_UTR 40.0%
CCCTAGGAGTCTTAAAACTA+AGG + chr8_1:3382054-3382073 None:intergenic 40.0%
GGGACACTTCTTGTCAATAT+AGG + chr8_1:3382531-3382550 None:intergenic 40.0%
GATTGAGACATTACCAGTGA+AGG + chr8_1:3382552-3382571 None:intergenic 40.0%
ATTCATTCCCCTTTCTTACC+AGG - chr8_1:3382672-3382691 Msa1173160:intron 40.0%
TTCATTCCCCTTTCTTACCA+GGG - chr8_1:3382673-3382692 Msa1173160:intron 40.0%
CGTGTGCAGGAATATTAGAA+TGG + chr8_1:3382780-3382799 None:intergenic 40.0%
AACCTCACTGTCTTAGAAAG+TGG + chr8_1:3382981-3383000 None:intergenic 40.0%
GGTCACGATCTCATCAAATT+TGG - chr8_1:3383142-3383161 Msa1173160:CDS 40.0%
GTTCGAGTACTAGTGTACAA+TGG - chr8_1:3383331-3383350 Msa1173160:intron 40.0%
CAGATTCAAGGTGCAAAACT+CGG - chr8_1:3381777-3381796 Msa1173160:three_prime_UTR 40.0%
TTCATCAACTCAACCAACCA+TGG - chr8_1:3381815-3381834 Msa1173160:three_prime_UTR 40.0%
!! CAAACTAACTTGTTCAGCCA+TGG + chr8_1:3381835-3381854 None:intergenic 40.0%
TTGCTTCAGAAAAGCCTTCT+CGG + chr8_1:3381948-3381967 None:intergenic 40.0%
!!! GCCTTAGTTTTAAGACTCCT+AGG - chr8_1:3382050-3382069 Msa1173160:three_prime_UTR 40.0%
CCCTAGGAGTCTTAAAACTA+AGG + chr8_1:3382054-3382073 None:intergenic 40.0%
GGGACACTTCTTGTCAATAT+AGG + chr8_1:3382531-3382550 None:intergenic 40.0%
GATTGAGACATTACCAGTGA+AGG + chr8_1:3382552-3382571 None:intergenic 40.0%
ATTCATTCCCCTTTCTTACC+AGG - chr8_1:3382672-3382691 Msa1173160:intron 40.0%
TTCATTCCCCTTTCTTACCA+GGG - chr8_1:3382673-3382692 Msa1173160:intron 40.0%
CGTGTGCAGGAATATTAGAA+TGG + chr8_1:3382780-3382799 None:intergenic 40.0%
AACCTCACTGTCTTAGAAAG+TGG + chr8_1:3382981-3383000 None:intergenic 40.0%
GGTCACGATCTCATCAAATT+TGG - chr8_1:3383142-3383161 Msa1173160:CDS 40.0%
GTTCGAGTACTAGTGTACAA+TGG - chr8_1:3383331-3383350 Msa1173160:intron 40.0%
!! CTAACTTGTTCAGCCATGGT+TGG + chr8_1:3381831-3381850 None:intergenic 45.0%
TGCTCGAAGATATCTGGGAA+GGG - chr8_1:3381983-3382002 Msa1173160:three_prime_UTR 45.0%
AAACCTTCAATGGCTTCCCT+AGG + chr8_1:3382070-3382089 None:intergenic 45.0%
! ACTGGTAATGTCTCAATCCG+AGG - chr8_1:3382554-3382573 Msa1173160:CDS 45.0%
ATCCGAGGTCGTATCATATC+CGG - chr8_1:3382569-3382588 Msa1173160:CDS 45.0%
TACCGGATATGATACGACCT+CGG + chr8_1:3382574-3382593 None:intergenic 45.0%
CTTAGCACTATGGCAAGTAC+CGG + chr8_1:3382591-3382610 None:intergenic 45.0%
AAACAAGGTGATACGTGTGC+AGG + chr8_1:3382793-3382812 None:intergenic 45.0%
! ATCACCTTGTTTCCGAGTGA+AGG - chr8_1:3382801-3382820 Msa1173160:intron 45.0%
CTTGTTTCCGAGTGAAGGAA+GGG - chr8_1:3382806-3382825 Msa1173160:intron 45.0%
TTGTTTCCGAGTGAAGGAAG+GGG - chr8_1:3382807-3382826 Msa1173160:intron 45.0%
GTATTGAAAGTCATCCCAGC+TGG - chr8_1:3383005-3383024 Msa1173160:intron 45.0%
CGTTTACAGGAGTCTGAGTT+TGG - chr8_1:3383057-3383076 Msa1173160:intron 45.0%
! TAAGCACTGCACTGCCATTA+TGG + chr8_1:3383261-3383280 None:intergenic 45.0%
!! CTAACTTGTTCAGCCATGGT+TGG + chr8_1:3381831-3381850 None:intergenic 45.0%
TGCTCGAAGATATCTGGGAA+GGG - chr8_1:3381983-3382002 Msa1173160:three_prime_UTR 45.0%
AAACCTTCAATGGCTTCCCT+AGG + chr8_1:3382070-3382089 None:intergenic 45.0%
! ACTGGTAATGTCTCAATCCG+AGG - chr8_1:3382554-3382573 Msa1173160:CDS 45.0%
ATCCGAGGTCGTATCATATC+CGG - chr8_1:3382569-3382588 Msa1173160:CDS 45.0%
TACCGGATATGATACGACCT+CGG + chr8_1:3382574-3382593 None:intergenic 45.0%
CTTAGCACTATGGCAAGTAC+CGG + chr8_1:3382591-3382610 None:intergenic 45.0%
AAACAAGGTGATACGTGTGC+AGG + chr8_1:3382793-3382812 None:intergenic 45.0%
! ATCACCTTGTTTCCGAGTGA+AGG - chr8_1:3382801-3382820 Msa1173160:intron 45.0%
CTTGTTTCCGAGTGAAGGAA+GGG - chr8_1:3382806-3382825 Msa1173160:intron 45.0%
TTGTTTCCGAGTGAAGGAAG+GGG - chr8_1:3382807-3382826 Msa1173160:intron 45.0%
GTATTGAAAGTCATCCCAGC+TGG - chr8_1:3383005-3383024 Msa1173160:intron 45.0%
CGTTTACAGGAGTCTGAGTT+TGG - chr8_1:3383057-3383076 Msa1173160:intron 45.0%
! TAAGCACTGCACTGCCATTA+TGG + chr8_1:3383261-3383280 None:intergenic 45.0%
GTGCTCGAAGATATCTGGGA+AGG - chr8_1:3381982-3382001 Msa1173160:three_prime_UTR 50.0%
GCTCGAAGATATCTGGGAAG+GGG - chr8_1:3381984-3382003 Msa1173160:three_prime_UTR 50.0%
AAGGGGAAGAGACCTGGAAA+GGG - chr8_1:3382001-3382020 Msa1173160:three_prime_UTR 50.0%
ACTCCTAGGGAAGCCATTGA+AGG - chr8_1:3382064-3382083 Msa1173160:three_prime_UTR 50.0%
GTGACATCATTTGCGTTGCG+AGG + chr8_1:3382390-3382409 None:intergenic 50.0%
!! GACAAGAAGTGTCCCTTCAC+TGG - chr8_1:3382536-3382555 Msa1173160:CDS 50.0%
ATACAGTAAACTGCGCACCC+TGG + chr8_1:3382693-3382712 None:intergenic 50.0%
CCTTCCTTCACTCGGAAACA+AGG + chr8_1:3382808-3382827 None:intergenic 50.0%
! CCTTGTTTCCGAGTGAAGGA+AGG - chr8_1:3382805-3382824 Msa1173160:intron 50.0%
CATGATCCCCTTCCTTCACT+CGG + chr8_1:3382816-3382835 None:intergenic 50.0%
!! GGCCACTTTCTAAGACAGTG+AGG - chr8_1:3382976-3382995 Msa1173160:intron 50.0%
ATCCCAGCTGGATCTTCTAG+TGG - chr8_1:3383017-3383036 Msa1173160:intron 50.0%
CACCACTAGAAGATCCAGCT+GGG + chr8_1:3383022-3383041 None:intergenic 50.0%
TGTAAACGCCTTCTTGGCAC+CGG + chr8_1:3383045-3383064 None:intergenic 50.0%
GACTCCTGTAAACGCCTTCT+TGG + chr8_1:3383051-3383070 None:intergenic 50.0%
GTGCTCGAAGATATCTGGGA+AGG - chr8_1:3381982-3382001 Msa1173160:three_prime_UTR 50.0%
GCTCGAAGATATCTGGGAAG+GGG - chr8_1:3381984-3382003 Msa1173160:three_prime_UTR 50.0%
AAGGGGAAGAGACCTGGAAA+GGG - chr8_1:3382001-3382020 Msa1173160:three_prime_UTR 50.0%
ACTCCTAGGGAAGCCATTGA+AGG - chr8_1:3382064-3382083 Msa1173160:three_prime_UTR 50.0%
GTGACATCATTTGCGTTGCG+AGG + chr8_1:3382390-3382409 None:intergenic 50.0%
!! GACAAGAAGTGTCCCTTCAC+TGG - chr8_1:3382536-3382555 Msa1173160:CDS 50.0%
ATACAGTAAACTGCGCACCC+TGG + chr8_1:3382693-3382712 None:intergenic 50.0%
CCTTCCTTCACTCGGAAACA+AGG + chr8_1:3382808-3382827 None:intergenic 50.0%
! CCTTGTTTCCGAGTGAAGGA+AGG - chr8_1:3382805-3382824 Msa1173160:intron 50.0%
CATGATCCCCTTCCTTCACT+CGG + chr8_1:3382816-3382835 None:intergenic 50.0%
!! GGCCACTTTCTAAGACAGTG+AGG - chr8_1:3382976-3382995 Msa1173160:intron 50.0%
ATCCCAGCTGGATCTTCTAG+TGG - chr8_1:3383017-3383036 Msa1173160:intron 50.0%
CACCACTAGAAGATCCAGCT+GGG + chr8_1:3383022-3383041 None:intergenic 50.0%
TGTAAACGCCTTCTTGGCAC+CGG + chr8_1:3383045-3383064 None:intergenic 50.0%
GACTCCTGTAAACGCCTTCT+TGG + chr8_1:3383051-3383070 None:intergenic 50.0%
GAAGGGGAAGAGACCTGGAA+AGG - chr8_1:3382000-3382019 Msa1173160:three_prime_UTR 55.0%
AAGCGATTGCCACCCTTTCC+AGG + chr8_1:3382016-3382035 None:intergenic 55.0%
TGCGCACCCTGGTAAGAAAG+GGG + chr8_1:3382682-3382701 None:intergenic 55.0%
CTGCGCACCCTGGTAAGAAA+GGG + chr8_1:3382683-3382702 None:intergenic 55.0%
ACTGCGCACCCTGGTAAGAA+AGG + chr8_1:3382684-3382703 None:intergenic 55.0%
GCACCACTAGAAGATCCAGC+TGG + chr8_1:3383023-3383042 None:intergenic 55.0%
GCTGGATCTTCTAGTGGTGC+CGG - chr8_1:3383023-3383042 Msa1173160:intron 55.0%
!! GGTGCCAAGAAGGCGTTTAC+AGG - chr8_1:3383044-3383063 Msa1173160:intron 55.0%
GAAGGGGAAGAGACCTGGAA+AGG - chr8_1:3382000-3382019 Msa1173160:three_prime_UTR 55.0%
AAGCGATTGCCACCCTTTCC+AGG + chr8_1:3382016-3382035 None:intergenic 55.0%
TGCGCACCCTGGTAAGAAAG+GGG + chr8_1:3382682-3382701 None:intergenic 55.0%
CTGCGCACCCTGGTAAGAAA+GGG + chr8_1:3382683-3382702 None:intergenic 55.0%
ACTGCGCACCCTGGTAAGAA+AGG + chr8_1:3382684-3382703 None:intergenic 55.0%
GCACCACTAGAAGATCCAGC+TGG + chr8_1:3383023-3383042 None:intergenic 55.0%
GCTGGATCTTCTAGTGGTGC+CGG - chr8_1:3383023-3383042 Msa1173160:intron 55.0%
!! GGTGCCAAGAAGGCGTTTAC+AGG - chr8_1:3383044-3383063 Msa1173160:intron 55.0%
TCTGGGAAGGGGAAGAGACC+TGG - chr8_1:3381995-3382014 Msa1173160:three_prime_UTR 60.0%
GGGAAGAGACCTGGAAAGGG+TGG - chr8_1:3382004-3382023 Msa1173160:three_prime_UTR 60.0%
!! TAGTGGTGCCGGTGCCAAGA+AGG - chr8_1:3383034-3383053 Msa1173160:intron 60.0%
TCTGGGAAGGGGAAGAGACC+TGG - chr8_1:3381995-3382014 Msa1173160:three_prime_UTR 60.0%
GGGAAGAGACCTGGAAAGGG+TGG - chr8_1:3382004-3382023 Msa1173160:three_prime_UTR 60.0%
!! TAGTGGTGCCGGTGCCAAGA+AGG - chr8_1:3383034-3383053 Msa1173160:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 3381775 3383411 3381775 ID=Msa1173160;Name=Msa1173160
chr8_1 mRNA 3381775 3383411 3381775 ID=Msa1173160-mRNA-1;Parent=Msa1173160;Name=Msa1173160-mRNA-1;_AED=0.03;_eAED=0.03;_QI=59|1|1|1|1|1|5|338|161
chr8_1 exon 3381775 3382209 3381775 ID=Msa1173160-mRNA-1:exon:1219;Parent=Msa1173160-mRNA-1
chr8_1 exon 3382332 3382421 3382332 ID=Msa1173160-mRNA-1:exon:1218;Parent=Msa1173160-mRNA-1
chr8_1 exon 3382517 3382661 3382517 ID=Msa1173160-mRNA-1:exon:1217;Parent=Msa1173160-mRNA-1
chr8_1 exon 3383101 3383242 3383101 ID=Msa1173160-mRNA-1:exon:1216;Parent=Msa1173160-mRNA-1
chr8_1 exon 3383341 3383411 3383341 ID=Msa1173160-mRNA-1:exon:1215;Parent=Msa1173160-mRNA-1
chr8_1 five_prime_UTR 3383353 3383411 3383353 ID=Msa1173160-mRNA-1:five_prime_utr;Parent=Msa1173160-mRNA-1
chr8_1 CDS 3383341 3383352 3383341 ID=Msa1173160-mRNA-1:cds;Parent=Msa1173160-mRNA-1
chr8_1 CDS 3383101 3383242 3383101 ID=Msa1173160-mRNA-1:cds;Parent=Msa1173160-mRNA-1
chr8_1 CDS 3382517 3382661 3382517 ID=Msa1173160-mRNA-1:cds;Parent=Msa1173160-mRNA-1
chr8_1 CDS 3382332 3382421 3382332 ID=Msa1173160-mRNA-1:cds;Parent=Msa1173160-mRNA-1
chr8_1 CDS 3382113 3382209 3382113 ID=Msa1173160-mRNA-1:cds;Parent=Msa1173160-mRNA-1
chr8_1 three_prime_UTR 3381775 3382112 3381775 ID=Msa1173160-mRNA-1:three_prime_utr;Parent=Msa1173160-mRNA-1
Gene Sequence

>Msa1173160

ATGGCTGAACAAACCGAGAAGGCTTTTCTGAAGCAAAATAAAGTGTTTTTGTGCTCGAAGATATCTGGGAAGGGGAAGAGACCTGGAAAGGGTGGCAATCGCTTTTATAAATCTGTTGGCCTTAGTTTTAAGACTCCTAGGGAAGCCATTGAAGGAACCTATATTGACAAGAAGTGTCCCTTCACTGGTAATGTCTCAATCCGAGGTCGTATCATATCCGGTACTTGCCATAGTGCTAAGATGAATAAAACCATTATTGTTAGGAGGAATTATCTTCATTTCATTAAGAAATATCAGAGGTATGAGAAGCGCCATTCTAATATTCCTGCACACGTATCACCTTGTTTCCGAGTGAAGGAAGGGGATCATGTTATTATTGGCCAATGCAGGCCACTTTCTAAGACAGTGAGGTTCAATGTATTGAAAGTCATCCCAGCTGGATCTTCTAGTGGTGCCGGTGCCAAGAAGGCGTTTACAGGAGTCTGA
Protein sequence

>Msa1173160

MAEQTEKAFLKQNKVFLCSKISGKGKRPGKGGNRFYKSVGLSFKTPREAIEGTYIDKKCPFTGNVSIRGRIISGTCHSAKMNKTIIVRRNYLHFIKKYQRYEKRHSNIPAHVSPCFRVKEGDHVIIGQCRPLSKTVRFNVLKVIPAGSSSGAGAKKAFTGV*