AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0052790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0052790 MtrunA17_Chr1g0152301 97.516 161 4 0 1 161 1 161 4.23e-115 322
Msa0052790 MtrunA17_Chr8g0372611 93.789 161 9 1 1 161 1 160 1.49e-98 280
Msa0052790 MtrunA17_Chr4g0065131 93.168 161 10 1 1 161 1 160 2.92e-98 280
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0052790 AT5G23740.1 87.578 161 18 1 1 161 1 159 9.39e-93 266
Msa0052790 AT3G48930.1 86.335 161 21 1 1 161 1 160 3.66e-91 262
Msa0052790 AT4G30800.1 86.335 161 20 1 1 161 1 159 8.33e-89 256

Find 58 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCACGATCTCATCAAATT+TGG 0.236368 1_2:-7106549 Msa0052790:three_prime_UTR
GAAGGGGATCATGTTATTAT+TGG 0.267190 1_2:-7106868 Msa0052790:CDS
CGTTTACAGGAGTCTGAGTT+TGG 0.301109 1_2:-7106634 Msa0052790:exon
ATAATTCCTCCTAACAATAA+TGG 0.308146 1_2:+7107070 None:intergenic
AAGCGATTGCCACCCTTTCC+AGG 0.318925 1_2:+7107678 None:intergenic
TAAGCACTGCACTGCCATTA+TGG 0.327802 1_2:+7106433 None:intergenic
TGAATAAAACCATTATTGTT+AGG 0.334061 1_2:-7107079 Msa0052790:CDS
GACTCCTGTAAACGCCTTCT+TGG 0.362038 1_2:+7106643 None:intergenic
GGTGCCAAGAAGGCGTTTAC+AGG 0.366657 1_2:-7106647 Msa0052790:CDS
GGGACACTTCTTGTCAATAT+AGG 0.373630 1_2:+7107163 None:intergenic
TTGCTTCAGAAAAGCCTTCT+CGG 0.396604 1_2:+7107746 None:intergenic
TTTGAGAAAGAAACCTTCAA+TGG 0.449940 1_2:+7107614 None:intergenic
CAGTAAAACATTTGAAACTA+AGG 0.459973 1_2:+7106581 None:intergenic
TACCTACTGCGGCAGATTCA+AGG 0.460205 1_2:-7107926 None:intergenic
CCCTAGGAGTCTTAAAACTA+AGG 0.476406 1_2:+7107640 None:intergenic
ACTCCTAGGGAAGCCATTGA+AGG 0.484194 1_2:-7107627 Msa0052790:intron
GCTGGATCTTCTAGTGGTGC+CGG 0.487132 1_2:-7106668 Msa0052790:CDS
ATGTTATTATTGGCCAATGC+AGG 0.499585 1_2:-7106858 Msa0052790:intron
TACAATGATCTAACCTGCAT+TGG 0.500383 1_2:+7106845 None:intergenic
ATCCGAGGTCGTATCATATC+CGG 0.504599 1_2:-7107122 Msa0052790:CDS
TCTGGGAAGGGGAAGAGACC+TGG 0.510724 1_2:-7107696 Msa0052790:CDS
AACCTCACTGTCTTAGAAAG+TGG 0.510906 1_2:+7106713 None:intergenic
TAGTGGTGCCGGTGCCAAGA+AGG 0.518324 1_2:-7106657 Msa0052790:CDS
CAGATTCAAGGTGCAAAACT+CGG 0.518925 1_2:-7107914 Msa0052790:five_prime_UTR
GACAAGAAGTGTCCCTTCAC+TGG 0.524673 1_2:-7107155 Msa0052790:CDS
ATCACCTTGTTTCCGAGTGA+AGG 0.529932 1_2:-7106890 Msa0052790:CDS
AAGGGGAAGAGACCTGGAAA+GGG 0.530695 1_2:-7107690 Msa0052790:CDS
CCTTGTTTCCGAGTGAAGGA+AGG 0.534218 1_2:-7106886 Msa0052790:CDS
CCTTCCTTCACTCGGAAACA+AGG 0.542963 1_2:+7106886 None:intergenic
TGCTCGAAGATATCTGGGAA+GGG 0.550883 1_2:-7107708 Msa0052790:CDS
CTTGTTTCCGAGTGAAGGAA+GGG 0.551830 1_2:-7106885 Msa0052790:CDS
ATAAAACCATTATTGTTAGG+AGG 0.551945 1_2:-7107076 Msa0052790:CDS
GTATTGAAAGTCATCCCAGC+TGG 0.559579 1_2:-7106686 Msa0052790:CDS
GGGAAGAGACCTGGAAAGGG+TGG 0.564985 1_2:-7107687 Msa0052790:CDS
TTTGAATTTGTAGACCGAGA+AGG 0.567346 1_2:-7107760 Msa0052790:intron
GCACCACTAGAAGATCCAGC+TGG 0.568341 1_2:+7106671 None:intergenic
CAAACTAACTTGTTCAGCCA+TGG 0.568984 1_2:+7107859 None:intergenic
ATCCCAGCTGGATCTTCTAG+TGG 0.570183 1_2:-7106674 Msa0052790:CDS
GAAGGGGAAGAGACCTGGAA+AGG 0.571357 1_2:-7107691 Msa0052790:CDS
CGTGTGCAGGAATATTAGAA+TGG 0.572181 1_2:+7106914 None:intergenic
CTTAGCACTATGGCAAGTAC+CGG 0.576116 1_2:+7107103 None:intergenic
CATGATCCCCTTCCTTCACT+CGG 0.580378 1_2:+7106878 None:intergenic
CTAACTTGTTCAGCCATGGT+TGG 0.581084 1_2:+7107863 None:intergenic
GTGCTCGAAGATATCTGGGA+AGG 0.582495 1_2:-7107709 Msa0052790:CDS
AAACCTTCAATGGCTTCCCT+AGG 0.582868 1_2:+7107624 None:intergenic
TTCATCAACTCAACCAACCA+TGG 0.588651 1_2:-7107876 Msa0052790:exon
AAACAAGGTGATACGTGTGC+AGG 0.596227 1_2:+7106901 None:intergenic
GCTCGAAGATATCTGGGAAG+GGG 0.598893 1_2:-7107707 Msa0052790:CDS
GATTGAGACATTACCAGTGA+AGG 0.618985 1_2:+7107142 None:intergenic
TTGTTTCCGAGTGAAGGAAG+GGG 0.622320 1_2:-7106884 Msa0052790:CDS
ATTGAGACATTACCAGTGAA+GGG 0.624989 1_2:+7107143 None:intergenic
CACCACTAGAAGATCCAGCT+GGG 0.629304 1_2:+7106672 None:intergenic
ACTGGTAATGTCTCAATCCG+AGG 0.640857 1_2:-7107137 Msa0052790:CDS
TGTAAACGCCTTCTTGGCAC+CGG 0.648607 1_2:+7106649 None:intergenic
ATTTCATTAAGAAATATCAG+AGG 0.666848 1_2:-7107043 Msa0052790:intron
GTTCGAGTACTAGTGTACAA+TGG 0.690452 1_2:-7106360 Msa0052790:three_prime_UTR
GGCCACTTTCTAAGACAGTG+AGG 0.709841 1_2:-7106715 Msa0052790:CDS
TACCGGATATGATACGACCT+CGG 0.712271 1_2:+7107120 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTATAGATTAATGTGA+AGG + chr1_2:7106388-7106407 None:intergenic 15.0%
!!! TTTTTTGTTTTGATAAATCT+TGG - chr1_2:7106410-7106429 Msa0052790:three_prime_UTR 15.0%
!!! ATTTTTATTTGAAAGTTGTT+CGG - chr1_2:7107916-7107935 Msa0052790:five_prime_UTR 15.0%
!! TAAATTATAGATTAATGTGA+AGG + chr1_2:7106388-7106407 None:intergenic 15.0%
!!! TTTTTTGTTTTGATAAATCT+TGG - chr1_2:7106410-7106429 Msa0052790:three_prime_UTR 15.0%
!!! TAGATTGGTTTATTATTGAT+AGG - chr1_2:7106719-7106738 Msa0052790:intron 20.0%
!! GTTACCAATTTATTTACATA+AGG - chr1_2:7106986-7107005 Msa0052790:intron 20.0%
!! GTATTAGATTACAAAAAGAA+AGG + chr1_2:7107013-7107032 None:intergenic 20.0%
!!! TACCTTTTTCTTAATCTTAT+AGG - chr1_2:7107030-7107049 Msa0052790:intron 20.0%
!! TTCCTATAAGATTAAGAAAA+AGG + chr1_2:7107035-7107054 None:intergenic 20.0%
!! TGAATAAAACCATTATTGTT+AGG - chr1_2:7107139-7107158 Msa0052790:CDS 20.0%
!! ATTTCATTAAGAAATATCAG+AGG - chr1_2:7107175-7107194 Msa0052790:intron 20.0%
!!! TTTTTATTTGAAAGTTGTTC+GGG - chr1_2:7107917-7107936 Msa0052790:five_prime_UTR 20.0%
!!! TAGATTGGTTTATTATTGAT+AGG - chr1_2:7106719-7106738 Msa0052790:intron 20.0%
!! GTTACCAATTTATTTACATA+AGG - chr1_2:7106986-7107005 Msa0052790:intron 20.0%
!! GTATTAGATTACAAAAAGAA+AGG + chr1_2:7107013-7107032 None:intergenic 20.0%
!!! TACCTTTTTCTTAATCTTAT+AGG - chr1_2:7107030-7107049 Msa0052790:intron 20.0%
!! TTCCTATAAGATTAAGAAAA+AGG + chr1_2:7107035-7107054 None:intergenic 20.0%
!! TGAATAAAACCATTATTGTT+AGG - chr1_2:7107139-7107158 Msa0052790:CDS 20.0%
!! ATTTCATTAAGAAATATCAG+AGG - chr1_2:7107175-7107194 Msa0052790:intron 20.0%
! TCTTGGATTTACTAACTAAT+TGG - chr1_2:7106427-7106446 Msa0052790:three_prime_UTR 25.0%
!!! AATCGCTTTTATAAATCTGT+TGG - chr1_2:7106555-7106574 Msa0052790:three_prime_UTR 25.0%
! TAAACCACGAATATGAAAAT+TGG + chr1_2:7106636-7106655 None:intergenic 25.0%
!! TTTTCATATTCGTGGTTTAA+TGG - chr1_2:7106637-7106656 Msa0052790:exon 25.0%
!!! CATTGTGTTTTAAGAATCAT+AGG - chr1_2:7106696-7106715 Msa0052790:CDS 25.0%
! TTTAAGAATCATAGGTAGAT+TGG - chr1_2:7106704-7106723 Msa0052790:CDS 25.0%
! AAGCTTATGAAAATAGCTTA+TGG + chr1_2:7106827-7106846 None:intergenic 25.0%
! AGGACTTATTAAAACAGTTT+AGG + chr1_2:7106897-7106916 None:intergenic 25.0%
! AGGACCTTATGTAAATAAAT+TGG + chr1_2:7106993-7107012 None:intergenic 25.0%
!! TTTTATTCATCTTAGCACTA+TGG + chr1_2:7107128-7107147 None:intergenic 25.0%
! ATAAAACCATTATTGTTAGG+AGG - chr1_2:7107142-7107161 Msa0052790:CDS 25.0%
! ATAATTCCTCCTAACAATAA+TGG + chr1_2:7107151-7107170 None:intergenic 25.0%
!!! TTGTTTTGTTTATCCAAGTA+AGG - chr1_2:7107427-7107446 Msa0052790:intron 25.0%
!! CAGTAAAACATTTGAAACTA+AGG + chr1_2:7107640-7107659 None:intergenic 25.0%
!!! TTTTATTTGAAAGTTGTTCG+GGG - chr1_2:7107918-7107937 Msa0052790:five_prime_UTR 25.0%
! TCTTGGATTTACTAACTAAT+TGG - chr1_2:7106427-7106446 Msa0052790:three_prime_UTR 25.0%
!!! AATCGCTTTTATAAATCTGT+TGG - chr1_2:7106555-7106574 Msa0052790:three_prime_UTR 25.0%
! TAAACCACGAATATGAAAAT+TGG + chr1_2:7106636-7106655 None:intergenic 25.0%
!! TTTTCATATTCGTGGTTTAA+TGG - chr1_2:7106637-7106656 Msa0052790:exon 25.0%
!!! CATTGTGTTTTAAGAATCAT+AGG - chr1_2:7106696-7106715 Msa0052790:CDS 25.0%
! TTTAAGAATCATAGGTAGAT+TGG - chr1_2:7106704-7106723 Msa0052790:CDS 25.0%
! AAGCTTATGAAAATAGCTTA+TGG + chr1_2:7106827-7106846 None:intergenic 25.0%
! AGGACTTATTAAAACAGTTT+AGG + chr1_2:7106897-7106916 None:intergenic 25.0%
! AGGACCTTATGTAAATAAAT+TGG + chr1_2:7106993-7107012 None:intergenic 25.0%
!! TTTTATTCATCTTAGCACTA+TGG + chr1_2:7107128-7107147 None:intergenic 25.0%
! ATAAAACCATTATTGTTAGG+AGG - chr1_2:7107142-7107161 Msa0052790:CDS 25.0%
! ATAATTCCTCCTAACAATAA+TGG + chr1_2:7107151-7107170 None:intergenic 25.0%
!!! TTGTTTTGTTTATCCAAGTA+AGG - chr1_2:7107427-7107446 Msa0052790:intron 25.0%
!! CAGTAAAACATTTGAAACTA+AGG + chr1_2:7107640-7107659 None:intergenic 25.0%
TTTGAGAAAGAAACCTTCAA+TGG + chr1_2:7106607-7106626 None:intergenic 30.0%
! GTTTCCAATTTTCATATTCG+TGG - chr1_2:7106629-7106648 Msa0052790:exon 30.0%
CTCTGTTTGGTAAAAAATAG+TGG + chr1_2:7106804-7106823 None:intergenic 30.0%
TGTGTATGCTTGTTCTTATT+AGG - chr1_2:7107270-7107289 Msa0052790:intron 30.0%
! AATGTTTTACTGTTCTGACT+TGG - chr1_2:7107648-7107667 Msa0052790:CDS 30.0%
! TGCAGTAATTTTTGCCATAA+TGG - chr1_2:7107771-7107790 Msa0052790:intron 30.0%
TTTGAGAAAGAAACCTTCAA+TGG + chr1_2:7106607-7106626 None:intergenic 30.0%
! GTTTCCAATTTTCATATTCG+TGG - chr1_2:7106629-7106648 Msa0052790:exon 30.0%
CTCTGTTTGGTAAAAAATAG+TGG + chr1_2:7106804-7106823 None:intergenic 30.0%
TGTGTATGCTTGTTCTTATT+AGG - chr1_2:7107270-7107289 Msa0052790:intron 30.0%
! AATGTTTTACTGTTCTGACT+TGG - chr1_2:7107648-7107667 Msa0052790:CDS 30.0%
! TGCAGTAATTTTTGCCATAA+TGG - chr1_2:7107771-7107790 Msa0052790:intron 30.0%
TTTGAATTTGTAGACCGAGA+AGG - chr1_2:7106458-7106477 Msa0052790:three_prime_UTR 35.0%
! TTTTTGTGCTCGAAGATATC+TGG - chr1_2:7106504-7106523 Msa0052790:three_prime_UTR 35.0%
! TTTTGTGCTCGAAGATATCT+GGG - chr1_2:7106505-7106524 Msa0052790:three_prime_UTR 35.0%
!!! CCTTAGTTTTAAGACTCCTA+GGG - chr1_2:7106578-7106597 Msa0052790:three_prime_UTR 35.0%
! AAATAGCTTATGGCTCTGTT+TGG + chr1_2:7106817-7106836 None:intergenic 35.0%
AAGCTTTCATGAAGAGCTTA+TGG - chr1_2:7106841-7106860 Msa0052790:intron 35.0%
AGATAAGCTCAAATAAGCCA+TGG - chr1_2:7106954-7106973 Msa0052790:intron 35.0%
! TTGGTAACATAAACATGCCA+TGG + chr1_2:7106974-7106993 None:intergenic 35.0%
ATTGAGACATTACCAGTGAA+GGG + chr1_2:7107078-7107097 None:intergenic 35.0%
TTACTGTATTTGTATGCCAC+AGG - chr1_2:7107231-7107250 Msa0052790:intron 35.0%
ACAAATAGTTAACACACCTG+TGG + chr1_2:7107250-7107269 None:intergenic 35.0%
GAAGGGGATCATGTTATTAT+TGG - chr1_2:7107350-7107369 Msa0052790:intron 35.0%
ATGTTATTATTGGCCAATGC+AGG - chr1_2:7107360-7107379 Msa0052790:intron 35.0%
TACAATGATCTAACCTGCAT+TGG + chr1_2:7107376-7107395 None:intergenic 35.0%
CACAAAAACAGTTCCTTACT+TGG + chr1_2:7107443-7107462 None:intergenic 35.0%
GCAGAAGTAAAGGTAATCAA+GGG + chr1_2:7107474-7107493 None:intergenic 35.0%
AGCAGAAGTAAAGGTAATCA+AGG + chr1_2:7107475-7107494 None:intergenic 35.0%
CTATGAACAAGCAGAAGTAA+AGG + chr1_2:7107484-7107503 None:intergenic 35.0%
CTTTACTTCTGCTTGTTCAT+AGG - chr1_2:7107482-7107501 Msa0052790:intron 35.0%
TTTGAATTTGTAGACCGAGA+AGG - chr1_2:7106458-7106477 Msa0052790:three_prime_UTR 35.0%
! TTTTTGTGCTCGAAGATATC+TGG - chr1_2:7106504-7106523 Msa0052790:three_prime_UTR 35.0%
! TTTTGTGCTCGAAGATATCT+GGG - chr1_2:7106505-7106524 Msa0052790:three_prime_UTR 35.0%
!!! CCTTAGTTTTAAGACTCCTA+GGG - chr1_2:7106578-7106597 Msa0052790:three_prime_UTR 35.0%
! AAATAGCTTATGGCTCTGTT+TGG + chr1_2:7106817-7106836 None:intergenic 35.0%
AAGCTTTCATGAAGAGCTTA+TGG - chr1_2:7106841-7106860 Msa0052790:intron 35.0%
AGATAAGCTCAAATAAGCCA+TGG - chr1_2:7106954-7106973 Msa0052790:intron 35.0%
! TTGGTAACATAAACATGCCA+TGG + chr1_2:7106974-7106993 None:intergenic 35.0%
ATTGAGACATTACCAGTGAA+GGG + chr1_2:7107078-7107097 None:intergenic 35.0%
TTACTGTATTTGTATGCCAC+AGG - chr1_2:7107231-7107250 Msa0052790:intron 35.0%
ACAAATAGTTAACACACCTG+TGG + chr1_2:7107250-7107269 None:intergenic 35.0%
GAAGGGGATCATGTTATTAT+TGG - chr1_2:7107350-7107369 Msa0052790:intron 35.0%
ATGTTATTATTGGCCAATGC+AGG - chr1_2:7107360-7107379 Msa0052790:intron 35.0%
TACAATGATCTAACCTGCAT+TGG + chr1_2:7107376-7107395 None:intergenic 35.0%
CACAAAAACAGTTCCTTACT+TGG + chr1_2:7107443-7107462 None:intergenic 35.0%
GCAGAAGTAAAGGTAATCAA+GGG + chr1_2:7107474-7107493 None:intergenic 35.0%
AGCAGAAGTAAAGGTAATCA+AGG + chr1_2:7107475-7107494 None:intergenic 35.0%
CTATGAACAAGCAGAAGTAA+AGG + chr1_2:7107484-7107503 None:intergenic 35.0%
CTTTACTTCTGCTTGTTCAT+AGG - chr1_2:7107482-7107501 Msa0052790:intron 35.0%
CAGATTCAAGGTGCAAAACT+CGG - chr1_2:7106304-7106323 Msa0052790:three_prime_UTR 40.0%
TTCATCAACTCAACCAACCA+TGG - chr1_2:7106342-7106361 Msa0052790:three_prime_UTR 40.0%
!! CAAACTAACTTGTTCAGCCA+TGG + chr1_2:7106362-7106381 None:intergenic 40.0%
TTGCTTCAGAAAAGCCTTCT+CGG + chr1_2:7106475-7106494 None:intergenic 40.0%
!!! GCCTTAGTTTTAAGACTCCT+AGG - chr1_2:7106577-7106596 Msa0052790:three_prime_UTR 40.0%
CCCTAGGAGTCTTAAAACTA+AGG + chr1_2:7106581-7106600 None:intergenic 40.0%
GGGACACTTCTTGTCAATAT+AGG + chr1_2:7107058-7107077 None:intergenic 40.0%
GATTGAGACATTACCAGTGA+AGG + chr1_2:7107079-7107098 None:intergenic 40.0%
ATTCATTCCCCTTTCTTACC+AGG - chr1_2:7107199-7107218 Msa0052790:intron 40.0%
TTCATTCCCCTTTCTTACCA+GGG - chr1_2:7107200-7107219 Msa0052790:intron 40.0%
CGTGTGCAGGAATATTAGAA+TGG + chr1_2:7107307-7107326 None:intergenic 40.0%
AACCTCACTGTCTTAGAAAG+TGG + chr1_2:7107508-7107527 None:intergenic 40.0%
GGTCACGATCTCATCAAATT+TGG - chr1_2:7107669-7107688 Msa0052790:CDS 40.0%
GTTCGAGTACTAGTGTACAA+TGG - chr1_2:7107858-7107877 Msa0052790:intron 40.0%
CAGATTCAAGGTGCAAAACT+CGG - chr1_2:7106304-7106323 Msa0052790:three_prime_UTR 40.0%
TTCATCAACTCAACCAACCA+TGG - chr1_2:7106342-7106361 Msa0052790:three_prime_UTR 40.0%
!! CAAACTAACTTGTTCAGCCA+TGG + chr1_2:7106362-7106381 None:intergenic 40.0%
TTGCTTCAGAAAAGCCTTCT+CGG + chr1_2:7106475-7106494 None:intergenic 40.0%
!!! GCCTTAGTTTTAAGACTCCT+AGG - chr1_2:7106577-7106596 Msa0052790:three_prime_UTR 40.0%
CCCTAGGAGTCTTAAAACTA+AGG + chr1_2:7106581-7106600 None:intergenic 40.0%
GGGACACTTCTTGTCAATAT+AGG + chr1_2:7107058-7107077 None:intergenic 40.0%
GATTGAGACATTACCAGTGA+AGG + chr1_2:7107079-7107098 None:intergenic 40.0%
ATTCATTCCCCTTTCTTACC+AGG - chr1_2:7107199-7107218 Msa0052790:intron 40.0%
TTCATTCCCCTTTCTTACCA+GGG - chr1_2:7107200-7107219 Msa0052790:intron 40.0%
CGTGTGCAGGAATATTAGAA+TGG + chr1_2:7107307-7107326 None:intergenic 40.0%
AACCTCACTGTCTTAGAAAG+TGG + chr1_2:7107508-7107527 None:intergenic 40.0%
GGTCACGATCTCATCAAATT+TGG - chr1_2:7107669-7107688 Msa0052790:CDS 40.0%
GTTCGAGTACTAGTGTACAA+TGG - chr1_2:7107858-7107877 Msa0052790:intron 40.0%
!! CTAACTTGTTCAGCCATGGT+TGG + chr1_2:7106358-7106377 None:intergenic 45.0%
TGCTCGAAGATATCTGGGAA+GGG - chr1_2:7106510-7106529 Msa0052790:three_prime_UTR 45.0%
AAACCTTCAATGGCTTCCCT+AGG + chr1_2:7106597-7106616 None:intergenic 45.0%
! ACTGGTAATGTCTCAATCCG+AGG - chr1_2:7107081-7107100 Msa0052790:CDS 45.0%
ATCCGAGGTCGTATCATATC+CGG - chr1_2:7107096-7107115 Msa0052790:CDS 45.0%
TACCGGATATGATACGACCT+CGG + chr1_2:7107101-7107120 None:intergenic 45.0%
CTTAGCACTATGGCAAGTAC+CGG + chr1_2:7107118-7107137 None:intergenic 45.0%
AAACAAGGTGATACGTGTGC+AGG + chr1_2:7107320-7107339 None:intergenic 45.0%
! ATCACCTTGTTTCCGAGTGA+AGG - chr1_2:7107328-7107347 Msa0052790:intron 45.0%
CTTGTTTCCGAGTGAAGGAA+GGG - chr1_2:7107333-7107352 Msa0052790:intron 45.0%
TTGTTTCCGAGTGAAGGAAG+GGG - chr1_2:7107334-7107353 Msa0052790:intron 45.0%
GTATTGAAAGTCATCCCAGC+TGG - chr1_2:7107532-7107551 Msa0052790:intron 45.0%
CGTTTACAGGAGTCTGAGTT+TGG - chr1_2:7107584-7107603 Msa0052790:intron 45.0%
! TAAGCACTGCACTGCCATTA+TGG + chr1_2:7107788-7107807 None:intergenic 45.0%
!! CTAACTTGTTCAGCCATGGT+TGG + chr1_2:7106358-7106377 None:intergenic 45.0%
TGCTCGAAGATATCTGGGAA+GGG - chr1_2:7106510-7106529 Msa0052790:three_prime_UTR 45.0%
AAACCTTCAATGGCTTCCCT+AGG + chr1_2:7106597-7106616 None:intergenic 45.0%
! ACTGGTAATGTCTCAATCCG+AGG - chr1_2:7107081-7107100 Msa0052790:CDS 45.0%
ATCCGAGGTCGTATCATATC+CGG - chr1_2:7107096-7107115 Msa0052790:CDS 45.0%
TACCGGATATGATACGACCT+CGG + chr1_2:7107101-7107120 None:intergenic 45.0%
CTTAGCACTATGGCAAGTAC+CGG + chr1_2:7107118-7107137 None:intergenic 45.0%
AAACAAGGTGATACGTGTGC+AGG + chr1_2:7107320-7107339 None:intergenic 45.0%
! ATCACCTTGTTTCCGAGTGA+AGG - chr1_2:7107328-7107347 Msa0052790:intron 45.0%
CTTGTTTCCGAGTGAAGGAA+GGG - chr1_2:7107333-7107352 Msa0052790:intron 45.0%
TTGTTTCCGAGTGAAGGAAG+GGG - chr1_2:7107334-7107353 Msa0052790:intron 45.0%
GTATTGAAAGTCATCCCAGC+TGG - chr1_2:7107532-7107551 Msa0052790:intron 45.0%
CGTTTACAGGAGTCTGAGTT+TGG - chr1_2:7107584-7107603 Msa0052790:intron 45.0%
! TAAGCACTGCACTGCCATTA+TGG + chr1_2:7107788-7107807 None:intergenic 45.0%
GTGCTCGAAGATATCTGGGA+AGG - chr1_2:7106509-7106528 Msa0052790:three_prime_UTR 50.0%
GCTCGAAGATATCTGGGAAG+GGG - chr1_2:7106511-7106530 Msa0052790:three_prime_UTR 50.0%
AAGGGGAAGAGACCTGGAAA+GGG - chr1_2:7106528-7106547 Msa0052790:three_prime_UTR 50.0%
ACTCCTAGGGAAGCCATTGA+AGG - chr1_2:7106591-7106610 Msa0052790:three_prime_UTR 50.0%
GTGACATCATTTGCGTTGCG+AGG + chr1_2:7106917-7106936 None:intergenic 50.0%
!! GACAAGAAGTGTCCCTTCAC+TGG - chr1_2:7107063-7107082 Msa0052790:CDS 50.0%
ATACAGTAAACTGCGCACCC+TGG + chr1_2:7107220-7107239 None:intergenic 50.0%
CCTTCCTTCACTCGGAAACA+AGG + chr1_2:7107335-7107354 None:intergenic 50.0%
! CCTTGTTTCCGAGTGAAGGA+AGG - chr1_2:7107332-7107351 Msa0052790:intron 50.0%
CATGATCCCCTTCCTTCACT+CGG + chr1_2:7107343-7107362 None:intergenic 50.0%
!! GGCCACTTTCTAAGACAGTG+AGG - chr1_2:7107503-7107522 Msa0052790:intron 50.0%
ATCCCAGCTGGATCTTCTAG+TGG - chr1_2:7107544-7107563 Msa0052790:intron 50.0%
CACCACTAGAAGATCCAGCT+GGG + chr1_2:7107549-7107568 None:intergenic 50.0%
TGTAAACGCCTTCTTGGCAC+CGG + chr1_2:7107572-7107591 None:intergenic 50.0%
GACTCCTGTAAACGCCTTCT+TGG + chr1_2:7107578-7107597 None:intergenic 50.0%
GTGCTCGAAGATATCTGGGA+AGG - chr1_2:7106509-7106528 Msa0052790:three_prime_UTR 50.0%
GCTCGAAGATATCTGGGAAG+GGG - chr1_2:7106511-7106530 Msa0052790:three_prime_UTR 50.0%
AAGGGGAAGAGACCTGGAAA+GGG - chr1_2:7106528-7106547 Msa0052790:three_prime_UTR 50.0%
ACTCCTAGGGAAGCCATTGA+AGG - chr1_2:7106591-7106610 Msa0052790:three_prime_UTR 50.0%
GTGACATCATTTGCGTTGCG+AGG + chr1_2:7106917-7106936 None:intergenic 50.0%
!! GACAAGAAGTGTCCCTTCAC+TGG - chr1_2:7107063-7107082 Msa0052790:CDS 50.0%
ATACAGTAAACTGCGCACCC+TGG + chr1_2:7107220-7107239 None:intergenic 50.0%
CCTTCCTTCACTCGGAAACA+AGG + chr1_2:7107335-7107354 None:intergenic 50.0%
! CCTTGTTTCCGAGTGAAGGA+AGG - chr1_2:7107332-7107351 Msa0052790:intron 50.0%
CATGATCCCCTTCCTTCACT+CGG + chr1_2:7107343-7107362 None:intergenic 50.0%
!! GGCCACTTTCTAAGACAGTG+AGG - chr1_2:7107503-7107522 Msa0052790:intron 50.0%
ATCCCAGCTGGATCTTCTAG+TGG - chr1_2:7107544-7107563 Msa0052790:intron 50.0%
CACCACTAGAAGATCCAGCT+GGG + chr1_2:7107549-7107568 None:intergenic 50.0%
TGTAAACGCCTTCTTGGCAC+CGG + chr1_2:7107572-7107591 None:intergenic 50.0%
GACTCCTGTAAACGCCTTCT+TGG + chr1_2:7107578-7107597 None:intergenic 50.0%
GAAGGGGAAGAGACCTGGAA+AGG - chr1_2:7106527-7106546 Msa0052790:three_prime_UTR 55.0%
AAGCGATTGCCACCCTTTCC+AGG + chr1_2:7106543-7106562 None:intergenic 55.0%
TGCGCACCCTGGTAAGAAAG+GGG + chr1_2:7107209-7107228 None:intergenic 55.0%
CTGCGCACCCTGGTAAGAAA+GGG + chr1_2:7107210-7107229 None:intergenic 55.0%
ACTGCGCACCCTGGTAAGAA+AGG + chr1_2:7107211-7107230 None:intergenic 55.0%
GCACCACTAGAAGATCCAGC+TGG + chr1_2:7107550-7107569 None:intergenic 55.0%
GCTGGATCTTCTAGTGGTGC+CGG - chr1_2:7107550-7107569 Msa0052790:intron 55.0%
!! GGTGCCAAGAAGGCGTTTAC+AGG - chr1_2:7107571-7107590 Msa0052790:intron 55.0%
GAAGGGGAAGAGACCTGGAA+AGG - chr1_2:7106527-7106546 Msa0052790:three_prime_UTR 55.0%
AAGCGATTGCCACCCTTTCC+AGG + chr1_2:7106543-7106562 None:intergenic 55.0%
TGCGCACCCTGGTAAGAAAG+GGG + chr1_2:7107209-7107228 None:intergenic 55.0%
CTGCGCACCCTGGTAAGAAA+GGG + chr1_2:7107210-7107229 None:intergenic 55.0%
ACTGCGCACCCTGGTAAGAA+AGG + chr1_2:7107211-7107230 None:intergenic 55.0%
GCACCACTAGAAGATCCAGC+TGG + chr1_2:7107550-7107569 None:intergenic 55.0%
GCTGGATCTTCTAGTGGTGC+CGG - chr1_2:7107550-7107569 Msa0052790:intron 55.0%
!! GGTGCCAAGAAGGCGTTTAC+AGG - chr1_2:7107571-7107590 Msa0052790:intron 55.0%
TCTGGGAAGGGGAAGAGACC+TGG - chr1_2:7106522-7106541 Msa0052790:three_prime_UTR 60.0%
GGGAAGAGACCTGGAAAGGG+TGG - chr1_2:7106531-7106550 Msa0052790:three_prime_UTR 60.0%
!! TAGTGGTGCCGGTGCCAAGA+AGG - chr1_2:7107561-7107580 Msa0052790:intron 60.0%
TCTGGGAAGGGGAAGAGACC+TGG - chr1_2:7106522-7106541 Msa0052790:three_prime_UTR 60.0%
GGGAAGAGACCTGGAAAGGG+TGG - chr1_2:7106531-7106550 Msa0052790:three_prime_UTR 60.0%
!! TAGTGGTGCCGGTGCCAAGA+AGG - chr1_2:7107561-7107580 Msa0052790:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 7106302 7107938 7106302 ID=Msa0052790;Name=Msa0052790
chr1_2 mRNA 7106302 7107938 7106302 ID=Msa0052790-mRNA-1;Parent=Msa0052790;Name=Msa0052790-mRNA-1;_AED=0.03;_eAED=0.03;_QI=59|1|1|1|1|1|5|338|161
chr1_2 exon 7106302 7106736 7106302 ID=Msa0052790-mRNA-1:exon:2424;Parent=Msa0052790-mRNA-1
chr1_2 exon 7106859 7106948 7106859 ID=Msa0052790-mRNA-1:exon:2423;Parent=Msa0052790-mRNA-1
chr1_2 exon 7107044 7107188 7107044 ID=Msa0052790-mRNA-1:exon:2422;Parent=Msa0052790-mRNA-1
chr1_2 exon 7107628 7107769 7107628 ID=Msa0052790-mRNA-1:exon:2421;Parent=Msa0052790-mRNA-1
chr1_2 exon 7107868 7107938 7107868 ID=Msa0052790-mRNA-1:exon:2420;Parent=Msa0052790-mRNA-1
chr1_2 five_prime_UTR 7107880 7107938 7107880 ID=Msa0052790-mRNA-1:five_prime_utr;Parent=Msa0052790-mRNA-1
chr1_2 CDS 7107868 7107879 7107868 ID=Msa0052790-mRNA-1:cds;Parent=Msa0052790-mRNA-1
chr1_2 CDS 7107628 7107769 7107628 ID=Msa0052790-mRNA-1:cds;Parent=Msa0052790-mRNA-1
chr1_2 CDS 7107044 7107188 7107044 ID=Msa0052790-mRNA-1:cds;Parent=Msa0052790-mRNA-1
chr1_2 CDS 7106859 7106948 7106859 ID=Msa0052790-mRNA-1:cds;Parent=Msa0052790-mRNA-1
chr1_2 CDS 7106640 7106736 7106640 ID=Msa0052790-mRNA-1:cds;Parent=Msa0052790-mRNA-1
chr1_2 three_prime_UTR 7106302 7106639 7106302 ID=Msa0052790-mRNA-1:three_prime_utr;Parent=Msa0052790-mRNA-1
Gene Sequence

>Msa0052790

ATGGCTGAACAAACCGAGAAGGCTTTTCTGAAGCAAAATAAAGTGTTTTTGTGCTCGAAGATATCTGGGAAGGGGAAGAGACCTGGAAAGGGTGGCAATCGCTTTTATAAATCTGTTGGCCTTAGTTTTAAGACTCCTAGGGAAGCCATTGAAGGAACCTATATTGACAAGAAGTGTCCCTTCACTGGTAATGTCTCAATCCGAGGTCGTATCATATCCGGTACTTGCCATAGTGCTAAGATGAATAAAACCATTATTGTTAGGAGGAATTATCTTCATTTCATTAAGAAATATCAGAGGTATGAGAAGCGCCATTCTAATATTCCTGCACACGTATCACCTTGTTTCCGAGTGAAGGAAGGGGATCATGTTATTATTGGCCAATGCAGGCCACTTTCTAAGACAGTGAGGTTCAATGTATTGAAAGTCATCCCAGCTGGATCTTCTAGTGGTGCCGGTGCCAAGAAGGCGTTTACAGGAGTCTGA
Protein sequence

>Msa0052790

MAEQTEKAFLKQNKVFLCSKISGKGKRPGKGGNRFYKSVGLSFKTPREAIEGTYIDKKCPFTGNVSIRGRIISGTCHSAKMNKTIIVRRNYLHFIKKYQRYEKRHSNIPAHVSPCFRVKEGDHVIIGQCRPLSKTVRFNVLKVIPAGSSSGAGAKKAFTGV*