AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1207200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207200 MtrunA17_Chr4g0056071 96.104 77 3 0 1 77 1 77 1.16e-45 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207200 AT4G17310.15 50.633 79 38 1 1 78 1 79 3.28e-14 62.8
Msa1207200 AT4G17310.16 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1207200 AT4G17310.7 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1207200 AT4G17310.14 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1207200 AT4G17310.8 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1207200 AT4G17310.10 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1207200 AT4G17310.2 50.633 79 38 1 1 78 1 79 4.78e-14 62.4
Msa1207200 AT4G17310.11 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.13 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.1 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.6 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.12 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.9 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.5 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.17 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.4 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1207200 AT4G17310.3 50.633 79 38 1 1 78 1 79 5.07e-14 62.4

Find 40 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCAGTGGTGGTTGGTTT+GGG 0.163477 8_1:-64650516 None:intergenic
CAGCAAGTATAACCACTTTC+TGG 0.185526 8_1:-64653558 None:intergenic
TCCAAGCTCCGGCGCAATTC+TGG 0.241996 8_1:-64650568 None:intergenic
TATGAATAAATATATCAATT+AGG 0.243988 8_1:-64653634 None:intergenic
TGTGGCAGTGGTGGTTGGTT+TGG 0.246581 8_1:-64650517 None:intergenic
AAGTTTGTAAGCTGGATTTA+TGG 0.256250 8_1:+64653517 Msa1207200:exon
ATCCAGCTTACAAACTTAAA+AGG 0.261444 8_1:-64653511 None:intergenic
ATGAATAAATATATCAATTA+GGG 0.347975 8_1:-64653633 None:intergenic
GGTTGGTTTGGGGATGGATG+AGG 0.393701 8_1:-64650505 None:intergenic
GGATTTAATTGCGGATTTGA+TGG 0.409263 8_1:-64650472 None:intergenic
AGTGGTGGTTGGTTTGGGGA+TGG 0.417037 8_1:-64650511 None:intergenic
AGCAAGTATAACCACTTTCT+GGG 0.418258 8_1:-64653557 None:intergenic
AAGAAATCATAAGCATAATC+TGG 0.422829 8_1:+64653761 Msa1207200:three_prime_UTR
TCCAGAATTGCGCCGGAGCT+TGG 0.422852 8_1:+64650567 Msa1207200:CDS
GATGGATGAGGTGCGAATGT+TGG 0.451724 8_1:-64650493 None:intergenic
GACTGCACGCATCCAAGCTC+CGG 0.453744 8_1:-64650579 None:intergenic
AATTGATATATTTATTCATA+CGG 0.472007 8_1:+64653637 Msa1207200:three_prime_UTR
TGGCAGTGGTGGTTGGTTTG+GGG 0.472598 8_1:-64650515 None:intergenic
GCCGCAACTGCGCTGTGAAG+TGG 0.483273 8_1:-64650609 None:intergenic
TGGTTAGATGATTAGTTCAA+AGG 0.492232 8_1:+64653781 Msa1207200:three_prime_UTR
GGTTAGATGATTAGTTCAAA+GGG 0.495301 8_1:+64653782 Msa1207200:three_prime_UTR
ATTTATGGTCTTTGTTACAT+TGG 0.498919 8_1:+64653532 Msa1207200:three_prime_UTR
GAAGATGTGGCAGTGGTGGT+TGG 0.504244 8_1:-64650522 None:intergenic
GCCACTTCACAGCGCAGTTG+CGG 0.509854 8_1:+64650608 Msa1207200:CDS
AGATGATTAGTTCAAAGGGA+GGG 0.543652 8_1:+64653786 Msa1207200:three_prime_UTR
TTTCTTCTAGCAAGTCACCT+TGG 0.551535 8_1:-64653476 None:intergenic
TTACATTGGTGCCCAGAAAG+TGG 0.557496 8_1:+64653546 Msa1207200:three_prime_UTR
ATTTGCATCCAGAATTGCGC+CGG 0.566247 8_1:+64650560 Msa1207200:CDS
GCGAATGTTGGATTTAATTG+CGG 0.569994 8_1:-64650481 None:intergenic
TGGAGAAGATGTGGCAGTGG+TGG 0.576473 8_1:-64650526 None:intergenic
CAATTCTGGATGCAAATGAT+CGG 0.592552 8_1:-64650554 None:intergenic
ACATAGTTCACGTTTGTAGT+CGG 0.600853 8_1:-64653679 None:intergenic
TAGATGATTAGTTCAAAGGG+AGG 0.605486 8_1:+64653785 Msa1207200:three_prime_UTR
GATTAACTTATAAGAACTAG+AGG 0.609972 8_1:-64653738 None:intergenic
AGCTTGGTCTATCAGTTCCA+AGG 0.611426 8_1:+64653459 Msa1207200:CDS
TAGTCACACACGTTTGTAGA+CGG 0.615341 8_1:-64653704 None:intergenic
GATGCAAATGATCGGCGAAG+TGG 0.621136 8_1:-64650546 None:intergenic
ACTTCACAGCGCAGTTGCGG+CGG 0.632388 8_1:+64650611 Msa1207200:CDS
TCGGCGAAGTGGAGAAGATG+TGG 0.637549 8_1:-64650535 None:intergenic
AAGTGGAGAAGATGTGGCAG+TGG 0.639588 8_1:-64650529 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTACAACAAAAAAAAA+AGG + chr8_1:64651032-64651051 Msa1207200:intron 10.0%
!!! TACTTTTCATTTTTAAATTA+CGG + chr8_1:64651972-64651991 Msa1207200:intron 10.0%
!!! AAAAGCTTTATATAATAATA+TGG - chr8_1:64652664-64652683 None:intergenic 10.0%
!! ATGAATAAATATATCAATTA+GGG - chr8_1:64653636-64653655 None:intergenic 10.0%
!! TATGAATAAATATATCAATT+AGG - chr8_1:64653637-64653656 None:intergenic 10.0%
!! AATTGATATATTTATTCATA+CGG + chr8_1:64653637-64653656 Msa1207200:three_prime_UTR 10.0%
!! ATATTTAATTGCAATTGAAT+TGG + chr8_1:64650879-64650898 Msa1207200:intron 15.0%
!! GAAAAAATAAAATTATTTGG+CGG + chr8_1:64651578-64651597 Msa1207200:intron 15.0%
!!! ATGTGATTTTCTAAAATTTT+TGG + chr8_1:64652542-64652561 Msa1207200:intron 15.0%
!! AAGAAAATTTGTGAATATTT+CGG - chr8_1:64652634-64652653 None:intergenic 15.0%
!! AATTTAGACGTAATAAAACA+TGG + chr8_1:64651062-64651081 Msa1207200:intron 20.0%
!! ATTTAGACGTAATAAAACAT+GGG + chr8_1:64651063-64651082 Msa1207200:intron 20.0%
!! GGGGAAAAAATAAAATTATT+TGG + chr8_1:64651575-64651594 Msa1207200:intron 20.0%
!! GTAATCTATTCAGAAATATT+AGG + chr8_1:64651753-64651772 Msa1207200:intron 20.0%
!!! TTTTGTTTGAGAAATGAAAA+GGG + chr8_1:64651880-64651899 Msa1207200:intron 20.0%
!!! AATCAATTTTATTTCCCTTA+GGG - chr8_1:64652167-64652186 None:intergenic 20.0%
!!! TAATCAATTTTATTTCCCTT+AGG - chr8_1:64652168-64652187 None:intergenic 20.0%
!! TTGTTCTTACTTAGTAAAAA+GGG - chr8_1:64653049-64653068 None:intergenic 20.0%
!! ATTGTTCTTACTTAGTAAAA+AGG - chr8_1:64653050-64653069 None:intergenic 20.0%
!!! TATGAACTTGTCTAAATTTT+TGG + chr8_1:64653351-64653370 Msa1207200:intron 20.0%
!!! TGTTTTATTTAGGTTTTCTA+CGG + chr8_1:64653396-64653415 Msa1207200:intron 20.0%
! AAAAAGAAAACATTAGAGTC+CGG - chr8_1:64650715-64650734 None:intergenic 25.0%
!!! TGAAAAGTTGATTTTGAATG+CGG - chr8_1:64650813-64650832 None:intergenic 25.0%
! TAAAAAAACTAATGCTACGA+GGG - chr8_1:64651156-64651175 None:intergenic 25.0%
! ATAAAAAAACTAATGCTACG+AGG - chr8_1:64651157-64651176 None:intergenic 25.0%
!!! ATACTTGATAATGAAGAAGA+AGG - chr8_1:64651693-64651712 None:intergenic 25.0%
!!! CTTTTGTTTGAGAAATGAAA+AGG + chr8_1:64651879-64651898 Msa1207200:intron 25.0%
! GAGAAACTTTAATTCTCTTA+GGG + chr8_1:64651905-64651924 Msa1207200:intron 25.0%
! ACAGAAAAAAGTAGAAAAAG+TGG + chr8_1:64652233-64652252 Msa1207200:intron 25.0%
! CAGAAAAAAGTAGAAAAAGT+GGG + chr8_1:64652234-64652253 Msa1207200:intron 25.0%
! AAAAATTCACAATGCATCTA+GGG - chr8_1:64652315-64652334 None:intergenic 25.0%
!!! AATTACTTAGAGCCATTTTT+GGG - chr8_1:64652368-64652387 None:intergenic 25.0%
!!! TAATTACTTAGAGCCATTTT+TGG - chr8_1:64652369-64652388 None:intergenic 25.0%
! GTTATGCAAAACAAAATGTA+TGG - chr8_1:64652434-64652453 None:intergenic 25.0%
! AATTCAGTGTAATTATGTGT+CGG - chr8_1:64652522-64652541 None:intergenic 25.0%
! TCTACCTAATGTAAACAAAT+GGG + chr8_1:64652721-64652740 Msa1207200:intron 25.0%
! ACTTGATATAATCTATCACA+TGG + chr8_1:64652808-64652827 Msa1207200:intron 25.0%
! ATTGAGTACAAACTATGAAA+CGG - chr8_1:64653194-64653213 None:intergenic 25.0%
! CTTAAGAGAAACATATGTTA+TGG - chr8_1:64653228-64653247 None:intergenic 25.0%
!!! CTCTAGAACAAATAAAACAA+AGG - chr8_1:64653441-64653460 None:intergenic 25.0%
! ATTTATGGTCTTTGTTACAT+TGG + chr8_1:64653532-64653551 Msa1207200:three_prime_UTR 25.0%
!!! ATACTTGCTGTAATTTTTTC+AGG + chr8_1:64653571-64653590 Msa1207200:three_prime_UTR 25.0%
! GATTAACTTATAAGAACTAG+AGG - chr8_1:64653741-64653760 None:intergenic 25.0%
! AAGAAATCATAAGCATAATC+TGG + chr8_1:64653761-64653780 Msa1207200:three_prime_UTR 25.0%
!!! AATTTTGACTCCATCAAAAA+TGG - chr8_1:64653847-64653866 None:intergenic 25.0%
AAAAGAAAACATTAGAGTCC+GGG - chr8_1:64650714-64650733 None:intergenic 30.0%
AACGTTGCAATAACTTAACA+AGG - chr8_1:64650935-64650954 None:intergenic 30.0%
!!! TATTGCAACGTTTGTTTTAC+TGG + chr8_1:64650943-64650962 Msa1207200:intron 30.0%
GGAAGTTATTGTATACACTT+GGG + chr8_1:64651102-64651121 Msa1207200:intron 30.0%
CTACTATAATAAGTGCTTGT+GGG - chr8_1:64651340-64651359 None:intergenic 30.0%
TCTACTATAATAAGTGCTTG+TGG - chr8_1:64651341-64651360 None:intergenic 30.0%
TCAATCAATAGAGTTTCCAT+TGG - chr8_1:64651465-64651484 None:intergenic 30.0%
GAAAGACTAACTGGAAAATT+TGG + chr8_1:64651616-64651635 Msa1207200:intron 30.0%
! TTTTCTTCAGGTAACAGAAA+AGG - chr8_1:64651789-64651808 None:intergenic 30.0%
! ACCTGAAGAAAAAGTTCTTT+TGG + chr8_1:64651797-64651816 Msa1207200:intron 30.0%
! TGAAGAAAAAGTTCTTTTGG+CGG + chr8_1:64651800-64651819 Msa1207200:intron 30.0%
TCTACAGATTTCCAAATAAC+TGG - chr8_1:64651831-64651850 None:intergenic 30.0%
! GAAATCTGTAGATTTTCCAT+AGG + chr8_1:64651839-64651858 Msa1207200:intron 30.0%
GGAGAAACTTTAATTCTCTT+AGG + chr8_1:64651904-64651923 Msa1207200:intron 30.0%
TCAGAGTTAATCACAACATA+AGG - chr8_1:64652208-64652227 None:intergenic 30.0%
! TGCATTATAGGGTTTGAAAT+GGG - chr8_1:64652274-64652293 None:intergenic 30.0%
CAAAAATTCACAATGCATCT+AGG - chr8_1:64652316-64652335 None:intergenic 30.0%
TCTTTAGTGTTTCCCAAAAA+TGG + chr8_1:64652353-64652372 Msa1207200:intron 30.0%
!!! TCTAAAATTTTTGGCTGTGT+CGG + chr8_1:64652551-64652570 Msa1207200:intron 30.0%
CTCTACCTAATGTAAACAAA+TGG + chr8_1:64652720-64652739 Msa1207200:intron 30.0%
!! AACACAAGTTTGTTTTCTGT+TGG + chr8_1:64652771-64652790 Msa1207200:intron 30.0%
ACTGAAAAAACACCTTTAGT+CGG + chr8_1:64652840-64652859 Msa1207200:intron 30.0%
! TGGTATAGAATTTTCTCCAA+AGG + chr8_1:64652876-64652895 Msa1207200:intron 30.0%
TCTTTCACAATATATCCAAC+AGG - chr8_1:64652906-64652925 None:intergenic 30.0%
!! ACAAAACAAAAGCTTTGAAG+TGG + chr8_1:64652925-64652944 Msa1207200:intron 30.0%
TATGTAGGGAAATTGAAACT+AGG + chr8_1:64652948-64652967 Msa1207200:intron 30.0%
CTTGATTTACAACAACATAC+TGG + chr8_1:64652974-64652993 Msa1207200:intron 30.0%
TTTCACCAGATACAATTGAT+GGG + chr8_1:64653089-64653108 Msa1207200:intron 30.0%
!!! ATTTTTGGTTCTTCAGTCTT+TGG + chr8_1:64653366-64653385 Msa1207200:intron 30.0%
!!! TGGCTCTTTGTGTTTTATTT+AGG + chr8_1:64653386-64653405 Msa1207200:intron 30.0%
!!! GTTTTATTTGTTCTAGAGCT+TGG + chr8_1:64653443-64653462 Msa1207200:intron 30.0%
ATCCAGCTTACAAACTTAAA+AGG - chr8_1:64653514-64653533 None:intergenic 30.0%
AAGTTTGTAAGCTGGATTTA+TGG + chr8_1:64653517-64653536 Msa1207200:exon 30.0%
! TGGTTAGATGATTAGTTCAA+AGG + chr8_1:64653781-64653800 Msa1207200:three_prime_UTR 30.0%
! GGTTAGATGATTAGTTCAAA+GGG + chr8_1:64653782-64653801 Msa1207200:three_prime_UTR 30.0%
!! ATGTTCGATTCCATTTTTGA+TGG + chr8_1:64653834-64653853 Msa1207200:three_prime_UTR 30.0%
GGATTTAATTGCGGATTTGA+TGG - chr8_1:64650475-64650494 None:intergenic 35.0%
GCGAATGTTGGATTTAATTG+CGG - chr8_1:64650484-64650503 None:intergenic 35.0%
CAATTCTGGATGCAAATGAT+CGG - chr8_1:64650557-64650576 None:intergenic 35.0%
GGGAAGTTATTGTATACACT+TGG + chr8_1:64651101-64651120 Msa1207200:intron 35.0%
! GCTGCTCTAATAAGCTATTT+TGG + chr8_1:64651253-64651272 Msa1207200:intron 35.0%
GTACAAAGTGAAAGACTAAC+TGG + chr8_1:64651607-64651626 Msa1207200:intron 35.0%
! CATAGTTAATTGGGAGTATG+CGG + chr8_1:64651651-64651670 Msa1207200:intron 35.0%
! ATAGTTAATTGGGAGTATGC+GGG + chr8_1:64651652-64651671 Msa1207200:intron 35.0%
ATGAAGAAGAAGGCCTTAAT+TGG - chr8_1:64651683-64651702 None:intergenic 35.0%
!! GCCAAAAGAACTTTTTCTTC+AGG - chr8_1:64651801-64651820 None:intergenic 35.0%
! GTAGATTTTCCATAGGATAG+CGG + chr8_1:64651846-64651865 Msa1207200:intron 35.0%
! TAGATTTTCCATAGGATAGC+GGG + chr8_1:64651847-64651866 Msa1207200:intron 35.0%
TGTTTGAGAAATGAAAAGGG+AGG + chr8_1:64651883-64651902 Msa1207200:intron 35.0%
TAATTCTCTTAGGGTGTGTT+TGG + chr8_1:64651914-64651933 Msa1207200:intron 35.0%
CTGCATTATAGGGTTTGAAA+TGG - chr8_1:64652275-64652294 None:intergenic 35.0%
TTGGGTCTTACTGCATTATA+GGG - chr8_1:64652285-64652304 None:intergenic 35.0%
ACTAAAGAAGAGCAGGTATA+TGG - chr8_1:64652341-64652360 None:intergenic 35.0%
TTTGCATAACCTATGAAGCA+CGG + chr8_1:64652444-64652463 Msa1207200:intron 35.0%
TAGTTATGCACTATGAAGCA+CGG - chr8_1:64652593-64652612 None:intergenic 35.0%
TTCATAGTGCATAACTAGTC+TGG + chr8_1:64652596-64652615 Msa1207200:intron 35.0%
! CTTTTGCTAGTGAGTTCAAT+TGG + chr8_1:64652679-64652698 Msa1207200:intron 35.0%
GTCACCCATTTGTTTACATT+AGG - chr8_1:64652728-64652747 None:intergenic 35.0%
ATATCCAACAGGAAAACCTT+TGG - chr8_1:64652895-64652914 None:intergenic 35.0%
!! AAAGCTTTGAAGTGGTATGT+AGG + chr8_1:64652933-64652952 Msa1207200:intron 35.0%
!! AAGCTTTGAAGTGGTATGTA+GGG + chr8_1:64652934-64652953 Msa1207200:intron 35.0%
CTTTCACCAGATACAATTGA+TGG + chr8_1:64653088-64653107 Msa1207200:intron 35.0%
TCTTAAGCAAACTGTATGAC+TGG + chr8_1:64653241-64653260 Msa1207200:intron 35.0%
!!! AGCCTTTTAAGTTTGTAAGC+TGG + chr8_1:64653509-64653528 Msa1207200:exon 35.0%
AGCAAGTATAACCACTTTCT+GGG - chr8_1:64653560-64653579 None:intergenic 35.0%
ACATAGTTCACGTTTGTAGT+CGG - chr8_1:64653682-64653701 None:intergenic 35.0%
! TAGATGATTAGTTCAAAGGG+AGG + chr8_1:64653785-64653804 Msa1207200:three_prime_UTR 35.0%
! AGATGATTAGTTCAAAGGGA+GGG + chr8_1:64653786-64653805 Msa1207200:three_prime_UTR 35.0%
AACTGTCAATCGCTTTCACA+GGG + chr8_1:64650669-64650688 Msa1207200:intron 40.0%
ACATGGGTGTAATGAAGATG+TGG + chr8_1:64651079-64651098 Msa1207200:intron 40.0%
CATGGGTGTAATGAAGATGT+GGG + chr8_1:64651080-64651099 Msa1207200:intron 40.0%
ATGGGTGTAATGAAGATGTG+GGG + chr8_1:64651081-64651100 Msa1207200:intron 40.0%
AAGTGCTTGTGGGACTATTT+GGG - chr8_1:64651330-64651349 None:intergenic 40.0%
TAAGTGCTTGTGGGACTATT+TGG - chr8_1:64651331-64651350 None:intergenic 40.0%
TGGTAAAGTCTACAGGTGAA+AGG - chr8_1:64651440-64651459 None:intergenic 40.0%
!! ATTGGCTTGGTAAAGTCTAC+AGG - chr8_1:64651447-64651466 None:intergenic 40.0%
TGTAGACTTTACCAAGCCAA+TGG + chr8_1:64651446-64651465 Msa1207200:intron 40.0%
! CAATAGAGTTTCCATTGGCT+TGG - chr8_1:64651460-64651479 None:intergenic 40.0%
!! CTTTGGTCTTGTTGTCTCTT+CGG + chr8_1:64651522-64651541 Msa1207200:intron 40.0%
!! TTTGGTCTTGTTGTCTCTTC+GGG + chr8_1:64651523-64651542 Msa1207200:intron 40.0%
CTGCAGGCACATAGTTAATT+GGG + chr8_1:64651642-64651661 Msa1207200:intron 40.0%
TTAGGGTGTGTTTGGATTGA+GGG + chr8_1:64651922-64651941 Msa1207200:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG + chr8_1:64651928-64651947 Msa1207200:intron 40.0%
TTTGGATTGAGGGTTTAGGA+GGG + chr8_1:64651932-64651951 Msa1207200:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr8_1:64651928-64651947 None:intergenic 40.0%
TTAGGGTGTGTTTGGATTGA+GGG - chr8_1:64651922-64651941 None:intergenic 40.0%
TCAATCCAAACACACCCTAA+GGG + chr8_1:64652150-64652169 Msa1207200:intron 40.0%
TATTTCCCTTAGGGTGTGTT+TGG - chr8_1:64652158-64652177 None:intergenic 40.0%
GTTGGGTCTTACTGCATTAT+AGG - chr8_1:64652286-64652305 None:intergenic 40.0%
! GTGTAATTATGTGTCGGTGT+CGG - chr8_1:64652516-64652535 None:intergenic 40.0%
ACACTCAAAGTTCCGACTAA+AGG - chr8_1:64652855-64652874 None:intergenic 40.0%
TAGTCGGAACTTTGAGTGTA+TGG + chr8_1:64652856-64652875 Msa1207200:intron 40.0%
! TTCTCCAAAGGTTTTCCTGT+TGG + chr8_1:64652888-64652907 Msa1207200:intron 40.0%
AGACATGCATGCTGTTCATA+AGG + chr8_1:64653117-64653136 Msa1207200:intron 40.0%
ATGCATGCTGTTCATAAGGT+GGG + chr8_1:64653121-64653140 Msa1207200:intron 40.0%
CAAACTATGAAACGGTCGTT+AGG - chr8_1:64653186-64653205 None:intergenic 40.0%
TGCAGTGATGCTATCATACA+AGG - chr8_1:64653320-64653339 None:intergenic 40.0%
TTTCTTCTAGCAAGTCACCT+TGG - chr8_1:64653479-64653498 None:intergenic 40.0%
CAGCAAGTATAACCACTTTC+TGG - chr8_1:64653561-64653580 None:intergenic 40.0%
TAGTCACACACGTTTGTAGA+CGG - chr8_1:64653707-64653726 None:intergenic 40.0%
ATTTGCATCCAGAATTGCGC+CGG + chr8_1:64650560-64650579 Msa1207200:CDS 45.0%
GAACTGTCAATCGCTTTCAC+AGG + chr8_1:64650668-64650687 Msa1207200:intron 45.0%
AAAACATTAGAGTCCGGGAG+AGG - chr8_1:64650709-64650728 None:intergenic 45.0%
GTATACACTTGGGTCTGACT+TGG + chr8_1:64651112-64651131 Msa1207200:intron 45.0%
TCATAGACCCTAGATCCATG+CGG + chr8_1:64651366-64651385 Msa1207200:intron 45.0%
AGAGACAACAAGACCAAAGG+AGG - chr8_1:64651521-64651540 None:intergenic 45.0%
CGAAGAGACAACAAGACCAA+AGG - chr8_1:64651524-64651543 None:intergenic 45.0%
! TGTTGTCTCTTCGGGTTTCT+AGG + chr8_1:64651531-64651550 Msa1207200:intron 45.0%
!!! CACGTGTAGGTTGGTTTTGT+GGG + chr8_1:64651554-64651573 Msa1207200:intron 45.0%
!!! ACGTGTAGGTTGGTTTTGTG+GGG + chr8_1:64651555-64651574 Msa1207200:intron 45.0%
! CTGGAAAATTTGGTTCCTGC+AGG + chr8_1:64651626-64651645 Msa1207200:intron 45.0%
CCAATTAACTATGTGCCTGC+AGG - chr8_1:64651644-64651663 None:intergenic 45.0%
CCTGCAGGCACATAGTTAAT+TGG + chr8_1:64651641-64651660 Msa1207200:intron 45.0%
AGAAGAAGGCCTTAATTGGC+TGG - chr8_1:64651679-64651698 None:intergenic 45.0%
ATAGAGAACCCGCTATCCTA+TGG - chr8_1:64651858-64651877 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG + chr8_1:64651921-64651940 Msa1207200:intron 45.0%
GTTTGGATTGAGGGTTTAGG+AGG + chr8_1:64651931-64651950 Msa1207200:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG + chr8_1:64651933-64651952 Msa1207200:intron 45.0%
TTGAGGGTTTAGGAGGAGAA+AGG - chr8_1:64652134-64652153 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr8_1:64651931-64651950 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG - chr8_1:64651921-64651940 None:intergenic 45.0%
CTCAATCCAAACACACCCTA+AGG + chr8_1:64652149-64652168 Msa1207200:intron 45.0%
!! TGCATCTAGGGTTAGCTGTT+GGG - chr8_1:64652303-64652322 None:intergenic 45.0%
!! ATGCATCTAGGGTTAGCTGT+TGG - chr8_1:64652304-64652323 None:intergenic 45.0%
GGGAAACACTAAAGAAGAGC+AGG - chr8_1:64652348-64652367 None:intergenic 45.0%
TATGAAGCACGGACACTTCT+CGG + chr8_1:64652455-64652474 Msa1207200:intron 45.0%
! GCATAACTAGTCTGGCATGA+TGG + chr8_1:64652604-64652623 Msa1207200:intron 45.0%
CACGTCCCATCAATTGTATC+TGG - chr8_1:64653097-64653116 None:intergenic 45.0%
CATGCATGCTGTTCATAAGG+TGG + chr8_1:64653120-64653139 Msa1207200:intron 45.0%
TTCTCGAACAGGTCTTCAGA+GGG - chr8_1:64653161-64653180 None:intergenic 45.0%
TTTCTCGAACAGGTCTTCAG+AGG - chr8_1:64653162-64653181 None:intergenic 45.0%
GTCGTTAGGATTTCTCGAAC+AGG - chr8_1:64653172-64653191 None:intergenic 45.0%
AGCTTGGTCTATCAGTTCCA+AGG + chr8_1:64653459-64653478 Msa1207200:CDS 45.0%
! TTACATTGGTGCCCAGAAAG+TGG + chr8_1:64653546-64653565 Msa1207200:three_prime_UTR 45.0%
GATGGATGAGGTGCGAATGT+TGG - chr8_1:64650496-64650515 None:intergenic 50.0%
AAGTGGAGAAGATGTGGCAG+TGG - chr8_1:64650532-64650551 None:intergenic 50.0%
GATGCAAATGATCGGCGAAG+TGG - chr8_1:64650549-64650568 None:intergenic 50.0%
! TCTACTAACTAAGCGCCGCA+TGG - chr8_1:64651384-64651403 None:intergenic 50.0%
AACAAGACCAAAGGAGGCTC+TGG - chr8_1:64651515-64651534 None:intergenic 50.0%
! GTTTCTAGGCACGTGTAGGT+TGG + chr8_1:64651545-64651564 Msa1207200:intron 50.0%
!!! GCACGTGTAGGTTGGTTTTG+TGG + chr8_1:64651553-64651572 Msa1207200:intron 50.0%
!!! CGTGTAGGTTGGTTTTGTGG+GGG + chr8_1:64651556-64651575 Msa1207200:intron 50.0%
TATGCGGGACCAGCCAATTA+AGG + chr8_1:64651667-64651686 Msa1207200:intron 50.0%
TGGCGGCTTCACCAGTTATT+TGG + chr8_1:64651817-64651836 Msa1207200:intron 50.0%
ATTGAGGGTTTAGGAGGGGA+AGG + chr8_1:64651937-64651956 Msa1207200:intron 50.0%
TTGAGGGTTTAGGAGGGGAA+GGG + chr8_1:64651938-64651957 Msa1207200:intron 50.0%
GGTTTAGGAGGAGAAAGGAG+GGG - chr8_1:64652129-64652148 None:intergenic 50.0%
GGGTTTAGGAGGAGAAAGGA+GGG - chr8_1:64652130-64652149 None:intergenic 50.0%
AGGGTTTAGGAGGAGAAAGG+AGG - chr8_1:64652131-64652150 None:intergenic 50.0%
!! GAGAAGTGTCCGTGCTTCAT+AGG - chr8_1:64652456-64652475 None:intergenic 50.0%
!! GGTTGGTTTGGGGATGGATG+AGG - chr8_1:64650508-64650527 None:intergenic 55.0%
!! AGTGGTGGTTGGTTTGGGGA+TGG - chr8_1:64650514-64650533 None:intergenic 55.0%
!! TGGCAGTGGTGGTTGGTTTG+GGG - chr8_1:64650518-64650537 None:intergenic 55.0%
!! GTGGCAGTGGTGGTTGGTTT+GGG - chr8_1:64650519-64650538 None:intergenic 55.0%
! TGTGGCAGTGGTGGTTGGTT+TGG - chr8_1:64650520-64650539 None:intergenic 55.0%
GAAGATGTGGCAGTGGTGGT+TGG - chr8_1:64650525-64650544 None:intergenic 55.0%
TGGAGAAGATGTGGCAGTGG+TGG - chr8_1:64650529-64650548 None:intergenic 55.0%
TCGGCGAAGTGGAGAAGATG+TGG - chr8_1:64650538-64650557 None:intergenic 55.0%
CACGTCTCAGCGTAACTTCG+AGG + chr8_1:64650646-64650665 Msa1207200:intron 55.0%
! CTAAGCGCCGCATGGATCTA+GGG - chr8_1:64651376-64651395 None:intergenic 55.0%
! ACTAAGCGCCGCATGGATCT+AGG - chr8_1:64651377-64651396 None:intergenic 55.0%
GAGTCTTCCAGAGCCTCCTT+TGG + chr8_1:64651505-64651524 Msa1207200:intron 55.0%
! TCGGGTTTCTAGGCACGTGT+AGG + chr8_1:64651541-64651560 Msa1207200:intron 55.0%
TAGGAGGAGAAAGGAGGGGA+GGG - chr8_1:64652125-64652144 None:intergenic 55.0%
TTAGGAGGAGAAAGGAGGGG+AGG - chr8_1:64652126-64652145 None:intergenic 55.0%
GCACGGACACTTCTCGGATA+AGG + chr8_1:64652461-64652480 Msa1207200:intron 55.0%
ACACGACACGTGTCTGACAC+GGG - chr8_1:64652493-64652512 None:intergenic 55.0%
GACACGACACGTGTCTGACA+CGG - chr8_1:64652494-64652513 None:intergenic 55.0%
GCACTATGAAGCACGGACAC+CGG - chr8_1:64652586-64652605 None:intergenic 55.0%
TCCAGAATTGCGCCGGAGCT+TGG + chr8_1:64650567-64650586 Msa1207200:CDS 60.0%
TCCAAGCTCCGGCGCAATTC+TGG - chr8_1:64650571-64650590 None:intergenic 60.0%
GACTGCACGCATCCAAGCTC+CGG - chr8_1:64650582-64650601 None:intergenic 60.0%
GCCACTTCACAGCGCAGTTG+CGG + chr8_1:64650608-64650627 Msa1207200:CDS 60.0%
ACTTCACAGCGCAGTTGCGG+CGG + chr8_1:64650611-64650630 Msa1207200:CDS 60.0%
ACTCTCTGCTGCACCTCTCC+CGG + chr8_1:64650693-64650712 Msa1207200:intron 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr8_1:64651941-64651960 Msa1207200:intron 60.0%
CACGACACGTGTCTGACACG+GGG - chr8_1:64652492-64652511 None:intergenic 60.0%
! GCTGTGTCGGTGTCCATGTC+CGG + chr8_1:64652564-64652583 Msa1207200:intron 60.0%
TGAAGCACGGACACCGGACA+TGG - chr8_1:64652580-64652599 None:intergenic 60.0%
GCCGCAACTGCGCTGTGAAG+TGG - chr8_1:64650612-64650631 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGCG+AGG + chr8_1:64651946-64651965 Msa1207200:intron 65.0%
TAGGAGGGGAAGGGAGGCGA+GGG + chr8_1:64651947-64651966 Msa1207200:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 64650426 64653901 64650426 ID=Msa1207200;Name=Msa1207200
chr8_1 mRNA 64650426 64653901 64650426 ID=Msa1207200-mRNA-1;Parent=Msa1207200;Name=Msa1207200-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0.5|1|0|0.5|2|383|96
chr8_1 exon 64650426 64650656 64650426 ID=Msa1207200-mRNA-1:exon:21133;Parent=Msa1207200-mRNA-1
chr8_1 exon 64653459 64653901 64653459 ID=Msa1207200-mRNA-1:exon:21134;Parent=Msa1207200-mRNA-1
chr8_1 CDS 64650426 64650656 64650426 ID=Msa1207200-mRNA-1:cds;Parent=Msa1207200-mRNA-1
chr8_1 CDS 64653459 64653518 64653459 ID=Msa1207200-mRNA-1:cds;Parent=Msa1207200-mRNA-1
chr8_1 three_prime_UTR 64653519 64653901 64653519 ID=Msa1207200-mRNA-1:three_prime_utr;Parent=Msa1207200-mRNA-1
Gene Sequence

>Msa1207200

ATGGCTTACGCATGCAGCAGAATCGCTCAAAGAGCATCAATTTCATCCATCAAATCCGCAATTAAATCCAACATTCGCACCTCATCCATCCCCAAACCAACCACCACTGCCACATCTTCTCCACTTCGCCGATCATTTGCATCCAGAATTGCGCCGGAGCTTGGATGCGTGCAGTCGATGTTGCCACTTCACAGCGCAGTTGCGGCGGCGAGAATGACTTCACGTCTCAGCAGCTTGGTCTATCAGTTCCAAGGTGACTTGCTAGAAGAAATATTGAAGAAAGCCTTTTAA
Protein sequence

>Msa1207200

MAYACSRIAQRASISSIKSAIKSNIRTSSIPKPTTTATSSPLRRSFASRIAPELGCVQSMLPLHSAVAAARMTSRLSSLVYQFQGDLLEEILKKAF*