AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1257450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257450 MtrunA17_Chr4g0056071 96.104 77 3 0 1 77 1 77 1.16e-45 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257450 AT4G17310.15 50.633 79 38 1 1 78 1 79 3.28e-14 62.8
Msa1257450 AT4G17310.16 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1257450 AT4G17310.7 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1257450 AT4G17310.14 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1257450 AT4G17310.8 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1257450 AT4G17310.10 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1257450 AT4G17310.2 50.633 79 38 1 1 78 1 79 4.78e-14 62.4
Msa1257450 AT4G17310.11 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.13 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.1 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.6 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.12 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.9 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.5 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.17 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.4 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1257450 AT4G17310.3 50.633 79 38 1 1 78 1 79 5.07e-14 62.4

Find 40 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCAGTGGTGGTTGGTTT+GGG 0.163477 8_2:-58076572 None:intergenic
CAGCAAGTATAACCACTTTC+TGG 0.185526 8_2:-58079614 None:intergenic
TCCAAGCTCCGGCGCAATTC+TGG 0.241996 8_2:-58076624 None:intergenic
TATGAATAAATATATCAATT+AGG 0.243988 8_2:-58079690 None:intergenic
TGTGGCAGTGGTGGTTGGTT+TGG 0.246581 8_2:-58076573 None:intergenic
AAGTTTGTAAGCTGGATTTA+TGG 0.256250 8_2:+58079573 Msa1257450:exon
ATCCAGCTTACAAACTTAAA+AGG 0.261444 8_2:-58079567 None:intergenic
ATGAATAAATATATCAATTA+GGG 0.347975 8_2:-58079689 None:intergenic
GGTTGGTTTGGGGATGGATG+AGG 0.393701 8_2:-58076561 None:intergenic
GGATTTAATTGCGGATTTGA+TGG 0.409263 8_2:-58076528 None:intergenic
AGTGGTGGTTGGTTTGGGGA+TGG 0.417037 8_2:-58076567 None:intergenic
AGCAAGTATAACCACTTTCT+GGG 0.418258 8_2:-58079613 None:intergenic
AAGAAATCATAAGCATAATC+TGG 0.422829 8_2:+58079817 Msa1257450:three_prime_UTR
TCCAGAATTGCGCCGGAGCT+TGG 0.422852 8_2:+58076623 Msa1257450:CDS
GATGGATGAGGTGCGAATGT+TGG 0.451724 8_2:-58076549 None:intergenic
GACTGCACGCATCCAAGCTC+CGG 0.453744 8_2:-58076635 None:intergenic
AATTGATATATTTATTCATA+CGG 0.472007 8_2:+58079693 Msa1257450:three_prime_UTR
TGGCAGTGGTGGTTGGTTTG+GGG 0.472598 8_2:-58076571 None:intergenic
GCCGCAACTGCGCTGTGAAG+TGG 0.483273 8_2:-58076665 None:intergenic
TGGTTAGATGATTAGTTCAA+AGG 0.492232 8_2:+58079837 Msa1257450:three_prime_UTR
GGTTAGATGATTAGTTCAAA+GGG 0.495301 8_2:+58079838 Msa1257450:three_prime_UTR
ATTTATGGTCTTTGTTACAT+TGG 0.498919 8_2:+58079588 Msa1257450:three_prime_UTR
GAAGATGTGGCAGTGGTGGT+TGG 0.504244 8_2:-58076578 None:intergenic
GCCACTTCACAGCGCAGTTG+CGG 0.509854 8_2:+58076664 Msa1257450:CDS
AGATGATTAGTTCAAAGGGA+GGG 0.543652 8_2:+58079842 Msa1257450:three_prime_UTR
TTTCTTCTAGCAAGTCACCT+TGG 0.551535 8_2:-58079532 None:intergenic
TTACATTGGTGCCCAGAAAG+TGG 0.557496 8_2:+58079602 Msa1257450:three_prime_UTR
ATTTGCATCCAGAATTGCGC+CGG 0.566247 8_2:+58076616 Msa1257450:CDS
GCGAATGTTGGATTTAATTG+CGG 0.569994 8_2:-58076537 None:intergenic
TGGAGAAGATGTGGCAGTGG+TGG 0.576473 8_2:-58076582 None:intergenic
CAATTCTGGATGCAAATGAT+CGG 0.592552 8_2:-58076610 None:intergenic
ACATAGTTCACGTTTGTAGT+CGG 0.600853 8_2:-58079735 None:intergenic
TAGATGATTAGTTCAAAGGG+AGG 0.605486 8_2:+58079841 Msa1257450:three_prime_UTR
GATTAACTTATAAGAACTAG+AGG 0.609972 8_2:-58079794 None:intergenic
AGCTTGGTCTATCAGTTCCA+AGG 0.611426 8_2:+58079515 Msa1257450:CDS
TAGTCACACACGTTTGTAGA+CGG 0.615341 8_2:-58079760 None:intergenic
GATGCAAATGATCGGCGAAG+TGG 0.621136 8_2:-58076602 None:intergenic
ACTTCACAGCGCAGTTGCGG+CGG 0.632388 8_2:+58076667 Msa1257450:CDS
TCGGCGAAGTGGAGAAGATG+TGG 0.637549 8_2:-58076591 None:intergenic
AAGTGGAGAAGATGTGGCAG+TGG 0.639588 8_2:-58076585 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTACAACAAAAAAAAA+AGG + chr8_2:58077088-58077107 Msa1257450:intron 10.0%
!!! TACTTTTCATTTTTAAATTA+CGG + chr8_2:58078028-58078047 Msa1257450:intron 10.0%
!!! AAAAGCTTTATATAATAATA+TGG - chr8_2:58078720-58078739 None:intergenic 10.0%
!! ATGAATAAATATATCAATTA+GGG - chr8_2:58079692-58079711 None:intergenic 10.0%
!! TATGAATAAATATATCAATT+AGG - chr8_2:58079693-58079712 None:intergenic 10.0%
!! AATTGATATATTTATTCATA+CGG + chr8_2:58079693-58079712 Msa1257450:three_prime_UTR 10.0%
!! ATATTTAATTGCAATTGAAT+TGG + chr8_2:58076935-58076954 Msa1257450:intron 15.0%
!! GAAAAAATAAAATTATTTGG+CGG + chr8_2:58077634-58077653 Msa1257450:intron 15.0%
!!! ATGTGATTTTCTAAAATTTT+TGG + chr8_2:58078598-58078617 Msa1257450:intron 15.0%
!! AAGAAAATTTGTGAATATTT+CGG - chr8_2:58078690-58078709 None:intergenic 15.0%
!! AATTTAGACGTAATAAAACA+TGG + chr8_2:58077118-58077137 Msa1257450:intron 20.0%
!! ATTTAGACGTAATAAAACAT+GGG + chr8_2:58077119-58077138 Msa1257450:intron 20.0%
!! GGGGAAAAAATAAAATTATT+TGG + chr8_2:58077631-58077650 Msa1257450:intron 20.0%
!! GTAATCTATTCAGAAATATT+AGG + chr8_2:58077809-58077828 Msa1257450:intron 20.0%
!!! TTTTGTTTGAGAAATGAAAA+GGG + chr8_2:58077936-58077955 Msa1257450:intron 20.0%
!!! AATCAATTTTATTTCCCTTA+GGG - chr8_2:58078223-58078242 None:intergenic 20.0%
!!! TAATCAATTTTATTTCCCTT+AGG - chr8_2:58078224-58078243 None:intergenic 20.0%
!! TTGTTCTTACTTAGTAAAAA+GGG - chr8_2:58079105-58079124 None:intergenic 20.0%
!! ATTGTTCTTACTTAGTAAAA+AGG - chr8_2:58079106-58079125 None:intergenic 20.0%
!!! TATGAACTTGTCTAAATTTT+TGG + chr8_2:58079407-58079426 Msa1257450:intron 20.0%
!!! TGTTTTATTTAGGTTTTCTA+CGG + chr8_2:58079452-58079471 Msa1257450:intron 20.0%
! AAAAAGAAAACATTAGAGTC+CGG - chr8_2:58076771-58076790 None:intergenic 25.0%
!!! TGAAAAGTTGATTTTGAATG+CGG - chr8_2:58076869-58076888 None:intergenic 25.0%
! TAAAAAAACTAATGCTACGA+GGG - chr8_2:58077212-58077231 None:intergenic 25.0%
! ATAAAAAAACTAATGCTACG+AGG - chr8_2:58077213-58077232 None:intergenic 25.0%
!!! ATACTTGATAATGAAGAAGA+AGG - chr8_2:58077749-58077768 None:intergenic 25.0%
!!! CTTTTGTTTGAGAAATGAAA+AGG + chr8_2:58077935-58077954 Msa1257450:intron 25.0%
! GAGAAACTTTAATTCTCTTA+GGG + chr8_2:58077961-58077980 Msa1257450:intron 25.0%
! ACAGAAAAAAGTAGAAAAAG+TGG + chr8_2:58078289-58078308 Msa1257450:intron 25.0%
! CAGAAAAAAGTAGAAAAAGT+GGG + chr8_2:58078290-58078309 Msa1257450:intron 25.0%
! AAAAATTCACAATGCATCTA+GGG - chr8_2:58078371-58078390 None:intergenic 25.0%
!!! AATTACTTAGAGCCATTTTT+GGG - chr8_2:58078424-58078443 None:intergenic 25.0%
!!! TAATTACTTAGAGCCATTTT+TGG - chr8_2:58078425-58078444 None:intergenic 25.0%
! GTTATGCAAAACAAAATGTA+TGG - chr8_2:58078490-58078509 None:intergenic 25.0%
! AATTCAGTGTAATTATGTGT+CGG - chr8_2:58078578-58078597 None:intergenic 25.0%
! TCTACCTAATGTAAACAAAT+GGG + chr8_2:58078777-58078796 Msa1257450:intron 25.0%
! ACTTGATATAATCTATCACA+TGG + chr8_2:58078864-58078883 Msa1257450:intron 25.0%
! ATTGAGTACAAACTATGAAA+CGG - chr8_2:58079250-58079269 None:intergenic 25.0%
! CTTAAGAGAAACATATGTTA+TGG - chr8_2:58079284-58079303 None:intergenic 25.0%
!!! CTCTAGAACAAATAAAACAA+AGG - chr8_2:58079497-58079516 None:intergenic 25.0%
! ATTTATGGTCTTTGTTACAT+TGG + chr8_2:58079588-58079607 Msa1257450:three_prime_UTR 25.0%
!!! ATACTTGCTGTAATTTTTTC+AGG + chr8_2:58079627-58079646 Msa1257450:three_prime_UTR 25.0%
! GATTAACTTATAAGAACTAG+AGG - chr8_2:58079797-58079816 None:intergenic 25.0%
! AAGAAATCATAAGCATAATC+TGG + chr8_2:58079817-58079836 Msa1257450:three_prime_UTR 25.0%
!!! AATTTTGACTCCATCAAAAA+TGG - chr8_2:58079903-58079922 None:intergenic 25.0%
AAAAGAAAACATTAGAGTCC+GGG - chr8_2:58076770-58076789 None:intergenic 30.0%
AACGTTGCAATAACTTAACA+AGG - chr8_2:58076991-58077010 None:intergenic 30.0%
!!! TATTGCAACGTTTGTTTTAC+TGG + chr8_2:58076999-58077018 Msa1257450:intron 30.0%
GGAAGTTATTGTATACACTT+GGG + chr8_2:58077158-58077177 Msa1257450:intron 30.0%
CTACTATAATAAGTGCTTGT+GGG - chr8_2:58077396-58077415 None:intergenic 30.0%
TCTACTATAATAAGTGCTTG+TGG - chr8_2:58077397-58077416 None:intergenic 30.0%
TCAATCAATAGAGTTTCCAT+TGG - chr8_2:58077521-58077540 None:intergenic 30.0%
GAAAGACTAACTGGAAAATT+TGG + chr8_2:58077672-58077691 Msa1257450:intron 30.0%
! TTTTCTTCAGGTAACAGAAA+AGG - chr8_2:58077845-58077864 None:intergenic 30.0%
! ACCTGAAGAAAAAGTTCTTT+TGG + chr8_2:58077853-58077872 Msa1257450:intron 30.0%
! TGAAGAAAAAGTTCTTTTGG+CGG + chr8_2:58077856-58077875 Msa1257450:intron 30.0%
TCTACAGATTTCCAAATAAC+TGG - chr8_2:58077887-58077906 None:intergenic 30.0%
! GAAATCTGTAGATTTTCCAT+AGG + chr8_2:58077895-58077914 Msa1257450:intron 30.0%
GGAGAAACTTTAATTCTCTT+AGG + chr8_2:58077960-58077979 Msa1257450:intron 30.0%
TCAGAGTTAATCACAACATA+AGG - chr8_2:58078264-58078283 None:intergenic 30.0%
! TGCATTATAGGGTTTGAAAT+GGG - chr8_2:58078330-58078349 None:intergenic 30.0%
CAAAAATTCACAATGCATCT+AGG - chr8_2:58078372-58078391 None:intergenic 30.0%
TCTTTAGTGTTTCCCAAAAA+TGG + chr8_2:58078409-58078428 Msa1257450:intron 30.0%
!!! TCTAAAATTTTTGGCTGTGT+CGG + chr8_2:58078607-58078626 Msa1257450:intron 30.0%
CTCTACCTAATGTAAACAAA+TGG + chr8_2:58078776-58078795 Msa1257450:intron 30.0%
!! AACACAAGTTTGTTTTCTGT+TGG + chr8_2:58078827-58078846 Msa1257450:intron 30.0%
ACTGAAAAAACACCTTTAGT+CGG + chr8_2:58078896-58078915 Msa1257450:intron 30.0%
! TGGTATAGAATTTTCTCCAA+AGG + chr8_2:58078932-58078951 Msa1257450:intron 30.0%
TCTTTCACAATATATCCAAC+AGG - chr8_2:58078962-58078981 None:intergenic 30.0%
!! ACAAAACAAAAGCTTTGAAG+TGG + chr8_2:58078981-58079000 Msa1257450:intron 30.0%
TATGTAGGGAAATTGAAACT+AGG + chr8_2:58079004-58079023 Msa1257450:intron 30.0%
CTTGATTTACAACAACATAC+TGG + chr8_2:58079030-58079049 Msa1257450:intron 30.0%
TTTCACCAGATACAATTGAT+GGG + chr8_2:58079145-58079164 Msa1257450:intron 30.0%
!!! ATTTTTGGTTCTTCAGTCTT+TGG + chr8_2:58079422-58079441 Msa1257450:intron 30.0%
!!! TGGCTCTTTGTGTTTTATTT+AGG + chr8_2:58079442-58079461 Msa1257450:intron 30.0%
!!! GTTTTATTTGTTCTAGAGCT+TGG + chr8_2:58079499-58079518 Msa1257450:intron 30.0%
ATCCAGCTTACAAACTTAAA+AGG - chr8_2:58079570-58079589 None:intergenic 30.0%
AAGTTTGTAAGCTGGATTTA+TGG + chr8_2:58079573-58079592 Msa1257450:exon 30.0%
! TGGTTAGATGATTAGTTCAA+AGG + chr8_2:58079837-58079856 Msa1257450:three_prime_UTR 30.0%
! GGTTAGATGATTAGTTCAAA+GGG + chr8_2:58079838-58079857 Msa1257450:three_prime_UTR 30.0%
!! ATGTTCGATTCCATTTTTGA+TGG + chr8_2:58079890-58079909 Msa1257450:three_prime_UTR 30.0%
GGATTTAATTGCGGATTTGA+TGG - chr8_2:58076531-58076550 None:intergenic 35.0%
GCGAATGTTGGATTTAATTG+CGG - chr8_2:58076540-58076559 None:intergenic 35.0%
CAATTCTGGATGCAAATGAT+CGG - chr8_2:58076613-58076632 None:intergenic 35.0%
GGGAAGTTATTGTATACACT+TGG + chr8_2:58077157-58077176 Msa1257450:intron 35.0%
! GCTGCTCTAATAAGCTATTT+TGG + chr8_2:58077309-58077328 Msa1257450:intron 35.0%
GTACAAAGTGAAAGACTAAC+TGG + chr8_2:58077663-58077682 Msa1257450:intron 35.0%
! CATAGTTAATTGGGAGTATG+CGG + chr8_2:58077707-58077726 Msa1257450:intron 35.0%
! ATAGTTAATTGGGAGTATGC+GGG + chr8_2:58077708-58077727 Msa1257450:intron 35.0%
ATGAAGAAGAAGGCCTTAAT+TGG - chr8_2:58077739-58077758 None:intergenic 35.0%
!! GCCAAAAGAACTTTTTCTTC+AGG - chr8_2:58077857-58077876 None:intergenic 35.0%
! GTAGATTTTCCATAGGATAG+CGG + chr8_2:58077902-58077921 Msa1257450:intron 35.0%
! TAGATTTTCCATAGGATAGC+GGG + chr8_2:58077903-58077922 Msa1257450:intron 35.0%
TGTTTGAGAAATGAAAAGGG+AGG + chr8_2:58077939-58077958 Msa1257450:intron 35.0%
TAATTCTCTTAGGGTGTGTT+TGG + chr8_2:58077970-58077989 Msa1257450:intron 35.0%
CTGCATTATAGGGTTTGAAA+TGG - chr8_2:58078331-58078350 None:intergenic 35.0%
TTGGGTCTTACTGCATTATA+GGG - chr8_2:58078341-58078360 None:intergenic 35.0%
ACTAAAGAAGAGCAGGTATA+TGG - chr8_2:58078397-58078416 None:intergenic 35.0%
TTTGCATAACCTATGAAGCA+CGG + chr8_2:58078500-58078519 Msa1257450:intron 35.0%
TAGTTATGCACTATGAAGCA+CGG - chr8_2:58078649-58078668 None:intergenic 35.0%
TTCATAGTGCATAACTAGTC+TGG + chr8_2:58078652-58078671 Msa1257450:intron 35.0%
! CTTTTGCTAGTGAGTTCAAT+TGG + chr8_2:58078735-58078754 Msa1257450:intron 35.0%
GTCACCCATTTGTTTACATT+AGG - chr8_2:58078784-58078803 None:intergenic 35.0%
ATATCCAACAGGAAAACCTT+TGG - chr8_2:58078951-58078970 None:intergenic 35.0%
!! AAAGCTTTGAAGTGGTATGT+AGG + chr8_2:58078989-58079008 Msa1257450:intron 35.0%
!! AAGCTTTGAAGTGGTATGTA+GGG + chr8_2:58078990-58079009 Msa1257450:intron 35.0%
CTTTCACCAGATACAATTGA+TGG + chr8_2:58079144-58079163 Msa1257450:intron 35.0%
TCTTAAGCAAACTGTATGAC+TGG + chr8_2:58079297-58079316 Msa1257450:intron 35.0%
!!! AGCCTTTTAAGTTTGTAAGC+TGG + chr8_2:58079565-58079584 Msa1257450:exon 35.0%
AGCAAGTATAACCACTTTCT+GGG - chr8_2:58079616-58079635 None:intergenic 35.0%
ACATAGTTCACGTTTGTAGT+CGG - chr8_2:58079738-58079757 None:intergenic 35.0%
! TAGATGATTAGTTCAAAGGG+AGG + chr8_2:58079841-58079860 Msa1257450:three_prime_UTR 35.0%
! AGATGATTAGTTCAAAGGGA+GGG + chr8_2:58079842-58079861 Msa1257450:three_prime_UTR 35.0%
AACTGTCAATCGCTTTCACA+GGG + chr8_2:58076725-58076744 Msa1257450:intron 40.0%
ACATGGGTGTAATGAAGATG+TGG + chr8_2:58077135-58077154 Msa1257450:intron 40.0%
CATGGGTGTAATGAAGATGT+GGG + chr8_2:58077136-58077155 Msa1257450:intron 40.0%
ATGGGTGTAATGAAGATGTG+GGG + chr8_2:58077137-58077156 Msa1257450:intron 40.0%
AAGTGCTTGTGGGACTATTT+GGG - chr8_2:58077386-58077405 None:intergenic 40.0%
TAAGTGCTTGTGGGACTATT+TGG - chr8_2:58077387-58077406 None:intergenic 40.0%
TGGTAAAGTCTACAGGTGAA+AGG - chr8_2:58077496-58077515 None:intergenic 40.0%
!! ATTGGCTTGGTAAAGTCTAC+AGG - chr8_2:58077503-58077522 None:intergenic 40.0%
TGTAGACTTTACCAAGCCAA+TGG + chr8_2:58077502-58077521 Msa1257450:intron 40.0%
! CAATAGAGTTTCCATTGGCT+TGG - chr8_2:58077516-58077535 None:intergenic 40.0%
!! CTTTGGTCTTGTTGTCTCTT+CGG + chr8_2:58077578-58077597 Msa1257450:intron 40.0%
!! TTTGGTCTTGTTGTCTCTTC+GGG + chr8_2:58077579-58077598 Msa1257450:intron 40.0%
CTGCAGGCACATAGTTAATT+GGG + chr8_2:58077698-58077717 Msa1257450:intron 40.0%
TTAGGGTGTGTTTGGATTGA+GGG + chr8_2:58077978-58077997 Msa1257450:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG + chr8_2:58077984-58078003 Msa1257450:intron 40.0%
TTTGGATTGAGGGTTTAGGA+GGG + chr8_2:58077988-58078007 Msa1257450:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr8_2:58077984-58078003 None:intergenic 40.0%
TTAGGGTGTGTTTGGATTGA+GGG - chr8_2:58077978-58077997 None:intergenic 40.0%
TCAATCCAAACACACCCTAA+GGG + chr8_2:58078206-58078225 Msa1257450:intron 40.0%
TATTTCCCTTAGGGTGTGTT+TGG - chr8_2:58078214-58078233 None:intergenic 40.0%
GTTGGGTCTTACTGCATTAT+AGG - chr8_2:58078342-58078361 None:intergenic 40.0%
! GTGTAATTATGTGTCGGTGT+CGG - chr8_2:58078572-58078591 None:intergenic 40.0%
ACACTCAAAGTTCCGACTAA+AGG - chr8_2:58078911-58078930 None:intergenic 40.0%
TAGTCGGAACTTTGAGTGTA+TGG + chr8_2:58078912-58078931 Msa1257450:intron 40.0%
! TTCTCCAAAGGTTTTCCTGT+TGG + chr8_2:58078944-58078963 Msa1257450:intron 40.0%
AGACATGCATGCTGTTCATA+AGG + chr8_2:58079173-58079192 Msa1257450:intron 40.0%
ATGCATGCTGTTCATAAGGT+GGG + chr8_2:58079177-58079196 Msa1257450:intron 40.0%
CAAACTATGAAACGGTCGTT+AGG - chr8_2:58079242-58079261 None:intergenic 40.0%
TGCAGTGATGCTATCATACA+AGG - chr8_2:58079376-58079395 None:intergenic 40.0%
TTTCTTCTAGCAAGTCACCT+TGG - chr8_2:58079535-58079554 None:intergenic 40.0%
CAGCAAGTATAACCACTTTC+TGG - chr8_2:58079617-58079636 None:intergenic 40.0%
TAGTCACACACGTTTGTAGA+CGG - chr8_2:58079763-58079782 None:intergenic 40.0%
ATTTGCATCCAGAATTGCGC+CGG + chr8_2:58076616-58076635 Msa1257450:CDS 45.0%
GAACTGTCAATCGCTTTCAC+AGG + chr8_2:58076724-58076743 Msa1257450:intron 45.0%
AAAACATTAGAGTCCGGGAG+AGG - chr8_2:58076765-58076784 None:intergenic 45.0%
GTATACACTTGGGTCTGACT+TGG + chr8_2:58077168-58077187 Msa1257450:intron 45.0%
TCATAGACCCTAGATCCATG+CGG + chr8_2:58077422-58077441 Msa1257450:intron 45.0%
AGAGACAACAAGACCAAAGG+AGG - chr8_2:58077577-58077596 None:intergenic 45.0%
CGAAGAGACAACAAGACCAA+AGG - chr8_2:58077580-58077599 None:intergenic 45.0%
! TGTTGTCTCTTCGGGTTTCT+AGG + chr8_2:58077587-58077606 Msa1257450:intron 45.0%
!!! CACGTGTAGGTTGGTTTTGT+GGG + chr8_2:58077610-58077629 Msa1257450:intron 45.0%
!!! ACGTGTAGGTTGGTTTTGTG+GGG + chr8_2:58077611-58077630 Msa1257450:intron 45.0%
! CTGGAAAATTTGGTTCCTGC+AGG + chr8_2:58077682-58077701 Msa1257450:intron 45.0%
CCAATTAACTATGTGCCTGC+AGG - chr8_2:58077700-58077719 None:intergenic 45.0%
CCTGCAGGCACATAGTTAAT+TGG + chr8_2:58077697-58077716 Msa1257450:intron 45.0%
AGAAGAAGGCCTTAATTGGC+TGG - chr8_2:58077735-58077754 None:intergenic 45.0%
ATAGAGAACCCGCTATCCTA+TGG - chr8_2:58077914-58077933 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG + chr8_2:58077977-58077996 Msa1257450:intron 45.0%
GTTTGGATTGAGGGTTTAGG+AGG + chr8_2:58077987-58078006 Msa1257450:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG + chr8_2:58077989-58078008 Msa1257450:intron 45.0%
TTGAGGGTTTAGGAGGAGAA+AGG - chr8_2:58078190-58078209 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr8_2:58077987-58078006 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG - chr8_2:58077977-58077996 None:intergenic 45.0%
CTCAATCCAAACACACCCTA+AGG + chr8_2:58078205-58078224 Msa1257450:intron 45.0%
!! TGCATCTAGGGTTAGCTGTT+GGG - chr8_2:58078359-58078378 None:intergenic 45.0%
!! ATGCATCTAGGGTTAGCTGT+TGG - chr8_2:58078360-58078379 None:intergenic 45.0%
GGGAAACACTAAAGAAGAGC+AGG - chr8_2:58078404-58078423 None:intergenic 45.0%
TATGAAGCACGGACACTTCT+CGG + chr8_2:58078511-58078530 Msa1257450:intron 45.0%
! GCATAACTAGTCTGGCATGA+TGG + chr8_2:58078660-58078679 Msa1257450:intron 45.0%
CACGTCCCATCAATTGTATC+TGG - chr8_2:58079153-58079172 None:intergenic 45.0%
CATGCATGCTGTTCATAAGG+TGG + chr8_2:58079176-58079195 Msa1257450:intron 45.0%
TTCTCGAACAGGTCTTCAGA+GGG - chr8_2:58079217-58079236 None:intergenic 45.0%
TTTCTCGAACAGGTCTTCAG+AGG - chr8_2:58079218-58079237 None:intergenic 45.0%
GTCGTTAGGATTTCTCGAAC+AGG - chr8_2:58079228-58079247 None:intergenic 45.0%
AGCTTGGTCTATCAGTTCCA+AGG + chr8_2:58079515-58079534 Msa1257450:CDS 45.0%
! TTACATTGGTGCCCAGAAAG+TGG + chr8_2:58079602-58079621 Msa1257450:three_prime_UTR 45.0%
GATGGATGAGGTGCGAATGT+TGG - chr8_2:58076552-58076571 None:intergenic 50.0%
AAGTGGAGAAGATGTGGCAG+TGG - chr8_2:58076588-58076607 None:intergenic 50.0%
GATGCAAATGATCGGCGAAG+TGG - chr8_2:58076605-58076624 None:intergenic 50.0%
! TCTACTAACTAAGCGCCGCA+TGG - chr8_2:58077440-58077459 None:intergenic 50.0%
AACAAGACCAAAGGAGGCTC+TGG - chr8_2:58077571-58077590 None:intergenic 50.0%
! GTTTCTAGGCACGTGTAGGT+TGG + chr8_2:58077601-58077620 Msa1257450:intron 50.0%
!!! GCACGTGTAGGTTGGTTTTG+TGG + chr8_2:58077609-58077628 Msa1257450:intron 50.0%
!!! CGTGTAGGTTGGTTTTGTGG+GGG + chr8_2:58077612-58077631 Msa1257450:intron 50.0%
TATGCGGGACCAGCCAATTA+AGG + chr8_2:58077723-58077742 Msa1257450:intron 50.0%
TGGCGGCTTCACCAGTTATT+TGG + chr8_2:58077873-58077892 Msa1257450:intron 50.0%
ATTGAGGGTTTAGGAGGGGA+AGG + chr8_2:58077993-58078012 Msa1257450:intron 50.0%
TTGAGGGTTTAGGAGGGGAA+GGG + chr8_2:58077994-58078013 Msa1257450:intron 50.0%
GGTTTAGGAGGAGAAAGGAG+GGG - chr8_2:58078185-58078204 None:intergenic 50.0%
GGGTTTAGGAGGAGAAAGGA+GGG - chr8_2:58078186-58078205 None:intergenic 50.0%
AGGGTTTAGGAGGAGAAAGG+AGG - chr8_2:58078187-58078206 None:intergenic 50.0%
!! GAGAAGTGTCCGTGCTTCAT+AGG - chr8_2:58078512-58078531 None:intergenic 50.0%
!! GGTTGGTTTGGGGATGGATG+AGG - chr8_2:58076564-58076583 None:intergenic 55.0%
!! AGTGGTGGTTGGTTTGGGGA+TGG - chr8_2:58076570-58076589 None:intergenic 55.0%
!! TGGCAGTGGTGGTTGGTTTG+GGG - chr8_2:58076574-58076593 None:intergenic 55.0%
!! GTGGCAGTGGTGGTTGGTTT+GGG - chr8_2:58076575-58076594 None:intergenic 55.0%
! TGTGGCAGTGGTGGTTGGTT+TGG - chr8_2:58076576-58076595 None:intergenic 55.0%
GAAGATGTGGCAGTGGTGGT+TGG - chr8_2:58076581-58076600 None:intergenic 55.0%
TGGAGAAGATGTGGCAGTGG+TGG - chr8_2:58076585-58076604 None:intergenic 55.0%
TCGGCGAAGTGGAGAAGATG+TGG - chr8_2:58076594-58076613 None:intergenic 55.0%
CACGTCTCAGCGTAACTTCG+AGG + chr8_2:58076702-58076721 Msa1257450:intron 55.0%
! CTAAGCGCCGCATGGATCTA+GGG - chr8_2:58077432-58077451 None:intergenic 55.0%
! ACTAAGCGCCGCATGGATCT+AGG - chr8_2:58077433-58077452 None:intergenic 55.0%
GAGTCTTCCAGAGCCTCCTT+TGG + chr8_2:58077561-58077580 Msa1257450:intron 55.0%
! TCGGGTTTCTAGGCACGTGT+AGG + chr8_2:58077597-58077616 Msa1257450:intron 55.0%
TAGGAGGAGAAAGGAGGGGA+GGG - chr8_2:58078181-58078200 None:intergenic 55.0%
TTAGGAGGAGAAAGGAGGGG+AGG - chr8_2:58078182-58078201 None:intergenic 55.0%
GCACGGACACTTCTCGGATA+AGG + chr8_2:58078517-58078536 Msa1257450:intron 55.0%
ACACGACACGTGTCTGACAC+GGG - chr8_2:58078549-58078568 None:intergenic 55.0%
GACACGACACGTGTCTGACA+CGG - chr8_2:58078550-58078569 None:intergenic 55.0%
GCACTATGAAGCACGGACAC+CGG - chr8_2:58078642-58078661 None:intergenic 55.0%
TCCAGAATTGCGCCGGAGCT+TGG + chr8_2:58076623-58076642 Msa1257450:CDS 60.0%
TCCAAGCTCCGGCGCAATTC+TGG - chr8_2:58076627-58076646 None:intergenic 60.0%
GACTGCACGCATCCAAGCTC+CGG - chr8_2:58076638-58076657 None:intergenic 60.0%
GCCACTTCACAGCGCAGTTG+CGG + chr8_2:58076664-58076683 Msa1257450:CDS 60.0%
ACTTCACAGCGCAGTTGCGG+CGG + chr8_2:58076667-58076686 Msa1257450:CDS 60.0%
ACTCTCTGCTGCACCTCTCC+CGG + chr8_2:58076749-58076768 Msa1257450:intron 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr8_2:58077997-58078016 Msa1257450:intron 60.0%
CACGACACGTGTCTGACACG+GGG - chr8_2:58078548-58078567 None:intergenic 60.0%
! GCTGTGTCGGTGTCCATGTC+CGG + chr8_2:58078620-58078639 Msa1257450:intron 60.0%
TGAAGCACGGACACCGGACA+TGG - chr8_2:58078636-58078655 None:intergenic 60.0%
GCCGCAACTGCGCTGTGAAG+TGG - chr8_2:58076668-58076687 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGCG+AGG + chr8_2:58078002-58078021 Msa1257450:intron 65.0%
TAGGAGGGGAAGGGAGGCGA+GGG + chr8_2:58078003-58078022 Msa1257450:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 58076482 58079957 58076482 ID=Msa1257450;Name=Msa1257450
chr8_2 mRNA 58076482 58079957 58076482 ID=Msa1257450-mRNA-1;Parent=Msa1257450;Name=Msa1257450-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0.5|1|1|1|2|383|96
chr8_2 exon 58076482 58076712 58076482 ID=Msa1257450-mRNA-1:exon:17942;Parent=Msa1257450-mRNA-1
chr8_2 exon 58079515 58079957 58079515 ID=Msa1257450-mRNA-1:exon:17943;Parent=Msa1257450-mRNA-1
chr8_2 CDS 58076482 58076712 58076482 ID=Msa1257450-mRNA-1:cds;Parent=Msa1257450-mRNA-1
chr8_2 CDS 58079515 58079574 58079515 ID=Msa1257450-mRNA-1:cds;Parent=Msa1257450-mRNA-1
chr8_2 three_prime_UTR 58079575 58079957 58079575 ID=Msa1257450-mRNA-1:three_prime_utr;Parent=Msa1257450-mRNA-1
Gene Sequence

>Msa1257450

ATGGCTTACGCATGCAGCAGAATCGCTCAAAGAGCATCAATTTCATCCATCAAATCCGCAATTAAATCCAACATTCGCACCTCATCCATCCCCAAACCAACCACCACTGCCACATCTTCTCCACTTCGCCGATCATTTGCATCCAGAATTGCGCCGGAGCTTGGATGCGTGCAGTCGATGTTGCCACTTCACAGCGCAGTTGCGGCGGCGAGAATGACTTCACGTCTCAGCAGCTTGGTCTATCAGTTCCAAGGTGACTTGCTAGAAGAAATATTGAAGAAAGCCTTTTAA
Protein sequence

>Msa1257450

MAYACSRIAQRASISSIKSAIKSNIRTSSIPKPTTTATSSPLRRSFASRIAPELGCVQSMLPLHSAVAAARMTSRLSSLVYQFQGDLLEEILKKAF*