AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1305260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305260 MtrunA17_Chr4g0056071 96.104 77 3 0 1 77 1 77 1.16e-45 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305260 AT4G17310.15 50.633 79 38 1 1 78 1 79 3.28e-14 62.8
Msa1305260 AT4G17310.16 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1305260 AT4G17310.7 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1305260 AT4G17310.14 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1305260 AT4G17310.8 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1305260 AT4G17310.10 50.633 79 38 1 1 78 1 79 3.78e-14 62.4
Msa1305260 AT4G17310.2 50.633 79 38 1 1 78 1 79 4.78e-14 62.4
Msa1305260 AT4G17310.11 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.13 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.1 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.6 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.12 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.9 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.5 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.17 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.4 50.633 79 38 1 1 78 1 79 5.07e-14 62.4
Msa1305260 AT4G17310.3 50.633 79 38 1 1 78 1 79 5.07e-14 62.4

Find 40 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCAGTGGTGGTTGGTTT+GGG 0.163477 8_3:-68633845 None:intergenic
CAGCAAGTATAACCACTTTC+TGG 0.185526 8_3:-68636887 None:intergenic
TCCAAGCTCCGGCGCAATTC+TGG 0.241996 8_3:-68633897 None:intergenic
TATGAATAAATATATCAATT+AGG 0.243988 8_3:-68636963 None:intergenic
TGTGGCAGTGGTGGTTGGTT+TGG 0.246581 8_3:-68633846 None:intergenic
AAGTTTGTAAGCTGGATTTA+TGG 0.256250 8_3:+68636846 Msa1305260:exon
ATCCAGCTTACAAACTTAAA+AGG 0.261444 8_3:-68636840 None:intergenic
ATGAATAAATATATCAATTA+GGG 0.347975 8_3:-68636962 None:intergenic
GGTTGGTTTGGGGATGGATG+AGG 0.393701 8_3:-68633834 None:intergenic
GGATTTAATTGCGGATTTGA+TGG 0.409263 8_3:-68633801 None:intergenic
AGTGGTGGTTGGTTTGGGGA+TGG 0.417037 8_3:-68633840 None:intergenic
AGCAAGTATAACCACTTTCT+GGG 0.418258 8_3:-68636886 None:intergenic
AAGAAATCATAAGCATAATC+TGG 0.422829 8_3:+68637090 Msa1305260:three_prime_UTR
TCCAGAATTGCGCCGGAGCT+TGG 0.422852 8_3:+68633896 Msa1305260:CDS
GATGGATGAGGTGCGAATGT+TGG 0.451724 8_3:-68633822 None:intergenic
GACTGCACGCATCCAAGCTC+CGG 0.453744 8_3:-68633908 None:intergenic
AATTGATATATTTATTCATA+CGG 0.472007 8_3:+68636966 Msa1305260:three_prime_UTR
TGGCAGTGGTGGTTGGTTTG+GGG 0.472598 8_3:-68633844 None:intergenic
GCCGCAACTGCGCTGTGAAG+TGG 0.483273 8_3:-68633938 None:intergenic
TGGTTAGATGATTAGTTCAA+AGG 0.492232 8_3:+68637110 Msa1305260:three_prime_UTR
GGTTAGATGATTAGTTCAAA+GGG 0.495301 8_3:+68637111 Msa1305260:three_prime_UTR
ATTTATGGTCTTTGTTACAT+TGG 0.498919 8_3:+68636861 Msa1305260:three_prime_UTR
GAAGATGTGGCAGTGGTGGT+TGG 0.504244 8_3:-68633851 None:intergenic
GCCACTTCACAGCGCAGTTG+CGG 0.509854 8_3:+68633937 Msa1305260:CDS
AGATGATTAGTTCAAAGGGA+GGG 0.543652 8_3:+68637115 Msa1305260:three_prime_UTR
TTTCTTCTAGCAAGTCACCT+TGG 0.551535 8_3:-68636805 None:intergenic
TTACATTGGTGCCCAGAAAG+TGG 0.557496 8_3:+68636875 Msa1305260:three_prime_UTR
ATTTGCATCCAGAATTGCGC+CGG 0.566247 8_3:+68633889 Msa1305260:CDS
GCGAATGTTGGATTTAATTG+CGG 0.569994 8_3:-68633810 None:intergenic
TGGAGAAGATGTGGCAGTGG+TGG 0.576473 8_3:-68633855 None:intergenic
CAATTCTGGATGCAAATGAT+CGG 0.592552 8_3:-68633883 None:intergenic
ACATAGTTCACGTTTGTAGT+CGG 0.600853 8_3:-68637008 None:intergenic
TAGATGATTAGTTCAAAGGG+AGG 0.605486 8_3:+68637114 Msa1305260:three_prime_UTR
GATTAACTTATAAGAACTAG+AGG 0.609972 8_3:-68637067 None:intergenic
AGCTTGGTCTATCAGTTCCA+AGG 0.611426 8_3:+68636788 Msa1305260:CDS
TAGTCACACACGTTTGTAGA+CGG 0.615341 8_3:-68637033 None:intergenic
GATGCAAATGATCGGCGAAG+TGG 0.621136 8_3:-68633875 None:intergenic
ACTTCACAGCGCAGTTGCGG+CGG 0.632388 8_3:+68633940 Msa1305260:CDS
TCGGCGAAGTGGAGAAGATG+TGG 0.637549 8_3:-68633864 None:intergenic
AAGTGGAGAAGATGTGGCAG+TGG 0.639588 8_3:-68633858 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTCATTTTTAAATTA+CGG + chr8_3:68635301-68635320 Msa1305260:intron 10.0%
!!! AAAAGCTTTATATAATAATA+TGG - chr8_3:68635993-68636012 None:intergenic 10.0%
!! ATGAATAAATATATCAATTA+GGG - chr8_3:68636965-68636984 None:intergenic 10.0%
!! TATGAATAAATATATCAATT+AGG - chr8_3:68636966-68636985 None:intergenic 10.0%
!! AATTGATATATTTATTCATA+CGG + chr8_3:68636966-68636985 Msa1305260:three_prime_UTR 10.0%
!! ATATTTAATTGCAATTGAAT+TGG + chr8_3:68634209-68634228 Msa1305260:intron 15.0%
!! CTAATTTACAACAAAAAAAA+AGG + chr8_3:68634361-68634380 Msa1305260:intron 15.0%
!! GAAAAAATAAAATTATTTGG+CGG + chr8_3:68634907-68634926 Msa1305260:intron 15.0%
!!! ATGTGATTTTCTAAAATTTT+TGG + chr8_3:68635871-68635890 Msa1305260:intron 15.0%
!! AAGAAAATTTGTGAATATTT+CGG - chr8_3:68635963-68635982 None:intergenic 15.0%
!! AATTTAGACGTAATAAAACA+TGG + chr8_3:68634391-68634410 Msa1305260:intron 20.0%
!! ATTTAGACGTAATAAAACAT+GGG + chr8_3:68634392-68634411 Msa1305260:intron 20.0%
!! GGGGAAAAAATAAAATTATT+TGG + chr8_3:68634904-68634923 Msa1305260:intron 20.0%
!! GTAATCTATTCAGAAATATT+AGG + chr8_3:68635082-68635101 Msa1305260:intron 20.0%
!!! TTTTGTTTGAGAAATGAAAA+GGG + chr8_3:68635209-68635228 Msa1305260:intron 20.0%
!!! AATCAATTTTATTTCCCTTA+GGG - chr8_3:68635496-68635515 None:intergenic 20.0%
!!! TAATCAATTTTATTTCCCTT+AGG - chr8_3:68635497-68635516 None:intergenic 20.0%
!! TTGTTCTTACTTAGTAAAAA+GGG - chr8_3:68636378-68636397 None:intergenic 20.0%
!! ATTGTTCTTACTTAGTAAAA+AGG - chr8_3:68636379-68636398 None:intergenic 20.0%
!!! TATGAACTTGTCTAAATTTT+TGG + chr8_3:68636680-68636699 Msa1305260:intron 20.0%
!!! TGTTTTATTTAGGTTTTCTA+CGG + chr8_3:68636725-68636744 Msa1305260:intron 20.0%
! AAAAAGAAAACATTAGAGTC+CGG - chr8_3:68634044-68634063 None:intergenic 25.0%
!!! TGAAAAGTTGATTTTGAATG+CGG - chr8_3:68634142-68634161 None:intergenic 25.0%
! TAAAAAAACTAATGCTACGA+GGG - chr8_3:68634485-68634504 None:intergenic 25.0%
! ATAAAAAAACTAATGCTACG+AGG - chr8_3:68634486-68634505 None:intergenic 25.0%
!!! ATACTTGATAATGAAGAAGA+AGG - chr8_3:68635022-68635041 None:intergenic 25.0%
!!! CTTTTGTTTGAGAAATGAAA+AGG + chr8_3:68635208-68635227 Msa1305260:intron 25.0%
! GAGAAACTTTAATTCTCTTA+GGG + chr8_3:68635234-68635253 Msa1305260:intron 25.0%
! ACAGAAAAAAGTAGAAAAAG+TGG + chr8_3:68635562-68635581 Msa1305260:intron 25.0%
! CAGAAAAAAGTAGAAAAAGT+GGG + chr8_3:68635563-68635582 Msa1305260:intron 25.0%
! AAAAATTCACAATGCATCTA+GGG - chr8_3:68635644-68635663 None:intergenic 25.0%
!!! AATTACTTAGAGCCATTTTT+GGG - chr8_3:68635697-68635716 None:intergenic 25.0%
!!! TAATTACTTAGAGCCATTTT+TGG - chr8_3:68635698-68635717 None:intergenic 25.0%
! GTTATGCAAAACAAAATGTA+TGG - chr8_3:68635763-68635782 None:intergenic 25.0%
! AATTCAGTGTAATTATGTGT+CGG - chr8_3:68635851-68635870 None:intergenic 25.0%
! TCTACCTAATGTAAACAAAT+GGG + chr8_3:68636050-68636069 Msa1305260:intron 25.0%
! ACTTGATATAATCTATCACA+TGG + chr8_3:68636137-68636156 Msa1305260:intron 25.0%
! ATTGAGTACAAACTATGAAA+CGG - chr8_3:68636523-68636542 None:intergenic 25.0%
! CTTAAGAGAAACATATGTTA+TGG - chr8_3:68636557-68636576 None:intergenic 25.0%
!!! CTCTAGAACAAATAAAACAA+AGG - chr8_3:68636770-68636789 None:intergenic 25.0%
! ATTTATGGTCTTTGTTACAT+TGG + chr8_3:68636861-68636880 Msa1305260:three_prime_UTR 25.0%
!!! ATACTTGCTGTAATTTTTTC+AGG + chr8_3:68636900-68636919 Msa1305260:three_prime_UTR 25.0%
! GATTAACTTATAAGAACTAG+AGG - chr8_3:68637070-68637089 None:intergenic 25.0%
! AAGAAATCATAAGCATAATC+TGG + chr8_3:68637090-68637109 Msa1305260:three_prime_UTR 25.0%
!!! AATTTTGACTCCATCAAAAA+TGG - chr8_3:68637176-68637195 None:intergenic 25.0%
AAAAGAAAACATTAGAGTCC+GGG - chr8_3:68634043-68634062 None:intergenic 30.0%
AACGTTGCAATAACTTAACA+AGG - chr8_3:68634265-68634284 None:intergenic 30.0%
!!! TATTGCAACGTTTGTTTTAC+TGG + chr8_3:68634273-68634292 Msa1305260:intron 30.0%
GGAAGTTATTGTATACACTT+GGG + chr8_3:68634431-68634450 Msa1305260:intron 30.0%
CTACTATAATAAGTGCTTGT+GGG - chr8_3:68634669-68634688 None:intergenic 30.0%
TCTACTATAATAAGTGCTTG+TGG - chr8_3:68634670-68634689 None:intergenic 30.0%
TCAATCAATAGAGTTTCCAT+TGG - chr8_3:68634794-68634813 None:intergenic 30.0%
GAAAGACTAACTGGAAAATT+TGG + chr8_3:68634945-68634964 Msa1305260:intron 30.0%
! TTTTCTTCAGGTAACAGAAA+AGG - chr8_3:68635118-68635137 None:intergenic 30.0%
! ACCTGAAGAAAAAGTTCTTT+TGG + chr8_3:68635126-68635145 Msa1305260:intron 30.0%
! TGAAGAAAAAGTTCTTTTGG+CGG + chr8_3:68635129-68635148 Msa1305260:intron 30.0%
TCTACAGATTTCCAAATAAC+TGG - chr8_3:68635160-68635179 None:intergenic 30.0%
! GAAATCTGTAGATTTTCCAT+AGG + chr8_3:68635168-68635187 Msa1305260:intron 30.0%
GGAGAAACTTTAATTCTCTT+AGG + chr8_3:68635233-68635252 Msa1305260:intron 30.0%
TCAGAGTTAATCACAACATA+AGG - chr8_3:68635537-68635556 None:intergenic 30.0%
! TGCATTATAGGGTTTGAAAT+GGG - chr8_3:68635603-68635622 None:intergenic 30.0%
CAAAAATTCACAATGCATCT+AGG - chr8_3:68635645-68635664 None:intergenic 30.0%
TCTTTAGTGTTTCCCAAAAA+TGG + chr8_3:68635682-68635701 Msa1305260:intron 30.0%
!!! TCTAAAATTTTTGGCTGTGT+CGG + chr8_3:68635880-68635899 Msa1305260:intron 30.0%
CTCTACCTAATGTAAACAAA+TGG + chr8_3:68636049-68636068 Msa1305260:intron 30.0%
!! AACACAAGTTTGTTTTCTGT+TGG + chr8_3:68636100-68636119 Msa1305260:intron 30.0%
ACTGAAAAAACACCTTTAGT+CGG + chr8_3:68636169-68636188 Msa1305260:intron 30.0%
! TGGTATAGAATTTTCTCCAA+AGG + chr8_3:68636205-68636224 Msa1305260:intron 30.0%
TCTTTCACAATATATCCAAC+AGG - chr8_3:68636235-68636254 None:intergenic 30.0%
!! ACAAAACAAAAGCTTTGAAG+TGG + chr8_3:68636254-68636273 Msa1305260:intron 30.0%
TATGTAGGGAAATTGAAACT+AGG + chr8_3:68636277-68636296 Msa1305260:intron 30.0%
CTTGATTTACAACAACATAC+TGG + chr8_3:68636303-68636322 Msa1305260:intron 30.0%
TTTCACCAGATACAATTGAT+GGG + chr8_3:68636418-68636437 Msa1305260:intron 30.0%
!!! ATTTTTGGTTCTTCAGTCTT+TGG + chr8_3:68636695-68636714 Msa1305260:intron 30.0%
!!! TGGCTCTTTGTGTTTTATTT+AGG + chr8_3:68636715-68636734 Msa1305260:intron 30.0%
!!! GTTTTATTTGTTCTAGAGCT+TGG + chr8_3:68636772-68636791 Msa1305260:intron 30.0%
ATCCAGCTTACAAACTTAAA+AGG - chr8_3:68636843-68636862 None:intergenic 30.0%
AAGTTTGTAAGCTGGATTTA+TGG + chr8_3:68636846-68636865 Msa1305260:exon 30.0%
! TGGTTAGATGATTAGTTCAA+AGG + chr8_3:68637110-68637129 Msa1305260:three_prime_UTR 30.0%
! GGTTAGATGATTAGTTCAAA+GGG + chr8_3:68637111-68637130 Msa1305260:three_prime_UTR 30.0%
!! ATGTTCGATTCCATTTTTGA+TGG + chr8_3:68637163-68637182 Msa1305260:three_prime_UTR 30.0%
GGATTTAATTGCGGATTTGA+TGG - chr8_3:68633804-68633823 None:intergenic 35.0%
GCGAATGTTGGATTTAATTG+CGG - chr8_3:68633813-68633832 None:intergenic 35.0%
CAATTCTGGATGCAAATGAT+CGG - chr8_3:68633886-68633905 None:intergenic 35.0%
GGGAAGTTATTGTATACACT+TGG + chr8_3:68634430-68634449 Msa1305260:intron 35.0%
! GCTGCTCTAATAAGCTATTT+TGG + chr8_3:68634582-68634601 Msa1305260:intron 35.0%
GTACAAAGTGAAAGACTAAC+TGG + chr8_3:68634936-68634955 Msa1305260:intron 35.0%
! CATAGTTAATTGGGAGTATG+CGG + chr8_3:68634980-68634999 Msa1305260:intron 35.0%
! ATAGTTAATTGGGAGTATGC+GGG + chr8_3:68634981-68635000 Msa1305260:intron 35.0%
ATGAAGAAGAAGGCCTTAAT+TGG - chr8_3:68635012-68635031 None:intergenic 35.0%
!! GCCAAAAGAACTTTTTCTTC+AGG - chr8_3:68635130-68635149 None:intergenic 35.0%
! GTAGATTTTCCATAGGATAG+CGG + chr8_3:68635175-68635194 Msa1305260:intron 35.0%
! TAGATTTTCCATAGGATAGC+GGG + chr8_3:68635176-68635195 Msa1305260:intron 35.0%
TGTTTGAGAAATGAAAAGGG+AGG + chr8_3:68635212-68635231 Msa1305260:intron 35.0%
TAATTCTCTTAGGGTGTGTT+TGG + chr8_3:68635243-68635262 Msa1305260:intron 35.0%
CTGCATTATAGGGTTTGAAA+TGG - chr8_3:68635604-68635623 None:intergenic 35.0%
TTGGGTCTTACTGCATTATA+GGG - chr8_3:68635614-68635633 None:intergenic 35.0%
ACTAAAGAAGAGCAGGTATA+TGG - chr8_3:68635670-68635689 None:intergenic 35.0%
TTTGCATAACCTATGAAGCA+CGG + chr8_3:68635773-68635792 Msa1305260:intron 35.0%
TAGTTATGCACTATGAAGCA+CGG - chr8_3:68635922-68635941 None:intergenic 35.0%
TTCATAGTGCATAACTAGTC+TGG + chr8_3:68635925-68635944 Msa1305260:intron 35.0%
! CTTTTGCTAGTGAGTTCAAT+TGG + chr8_3:68636008-68636027 Msa1305260:intron 35.0%
GTCACCCATTTGTTTACATT+AGG - chr8_3:68636057-68636076 None:intergenic 35.0%
ATATCCAACAGGAAAACCTT+TGG - chr8_3:68636224-68636243 None:intergenic 35.0%
!! AAAGCTTTGAAGTGGTATGT+AGG + chr8_3:68636262-68636281 Msa1305260:intron 35.0%
!! AAGCTTTGAAGTGGTATGTA+GGG + chr8_3:68636263-68636282 Msa1305260:intron 35.0%
CTTTCACCAGATACAATTGA+TGG + chr8_3:68636417-68636436 Msa1305260:intron 35.0%
TCTTAAGCAAACTGTATGAC+TGG + chr8_3:68636570-68636589 Msa1305260:intron 35.0%
!!! AGCCTTTTAAGTTTGTAAGC+TGG + chr8_3:68636838-68636857 Msa1305260:exon 35.0%
AGCAAGTATAACCACTTTCT+GGG - chr8_3:68636889-68636908 None:intergenic 35.0%
ACATAGTTCACGTTTGTAGT+CGG - chr8_3:68637011-68637030 None:intergenic 35.0%
! TAGATGATTAGTTCAAAGGG+AGG + chr8_3:68637114-68637133 Msa1305260:three_prime_UTR 35.0%
! AGATGATTAGTTCAAAGGGA+GGG + chr8_3:68637115-68637134 Msa1305260:three_prime_UTR 35.0%
AACTGTCAATCGCTTTCACA+GGG + chr8_3:68633998-68634017 Msa1305260:intron 40.0%
ACATGGGTGTAATGAAGATG+TGG + chr8_3:68634408-68634427 Msa1305260:intron 40.0%
CATGGGTGTAATGAAGATGT+GGG + chr8_3:68634409-68634428 Msa1305260:intron 40.0%
ATGGGTGTAATGAAGATGTG+GGG + chr8_3:68634410-68634429 Msa1305260:intron 40.0%
AAGTGCTTGTGGGACTATTT+GGG - chr8_3:68634659-68634678 None:intergenic 40.0%
TAAGTGCTTGTGGGACTATT+TGG - chr8_3:68634660-68634679 None:intergenic 40.0%
TGGTAAAGTCTACAGGTGAA+AGG - chr8_3:68634769-68634788 None:intergenic 40.0%
!! ATTGGCTTGGTAAAGTCTAC+AGG - chr8_3:68634776-68634795 None:intergenic 40.0%
TGTAGACTTTACCAAGCCAA+TGG + chr8_3:68634775-68634794 Msa1305260:intron 40.0%
! CAATAGAGTTTCCATTGGCT+TGG - chr8_3:68634789-68634808 None:intergenic 40.0%
!! CTTTGGTCTTGTTGTCTCTT+CGG + chr8_3:68634851-68634870 Msa1305260:intron 40.0%
!! TTTGGTCTTGTTGTCTCTTC+GGG + chr8_3:68634852-68634871 Msa1305260:intron 40.0%
CTGCAGGCACATAGTTAATT+GGG + chr8_3:68634971-68634990 Msa1305260:intron 40.0%
TTAGGGTGTGTTTGGATTGA+GGG + chr8_3:68635251-68635270 Msa1305260:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG + chr8_3:68635257-68635276 Msa1305260:intron 40.0%
TTTGGATTGAGGGTTTAGGA+GGG + chr8_3:68635261-68635280 Msa1305260:intron 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr8_3:68635257-68635276 None:intergenic 40.0%
TTAGGGTGTGTTTGGATTGA+GGG - chr8_3:68635251-68635270 None:intergenic 40.0%
TCAATCCAAACACACCCTAA+GGG + chr8_3:68635479-68635498 Msa1305260:intron 40.0%
TATTTCCCTTAGGGTGTGTT+TGG - chr8_3:68635487-68635506 None:intergenic 40.0%
GTTGGGTCTTACTGCATTAT+AGG - chr8_3:68635615-68635634 None:intergenic 40.0%
! GTGTAATTATGTGTCGGTGT+CGG - chr8_3:68635845-68635864 None:intergenic 40.0%
ACACTCAAAGTTCCGACTAA+AGG - chr8_3:68636184-68636203 None:intergenic 40.0%
TAGTCGGAACTTTGAGTGTA+TGG + chr8_3:68636185-68636204 Msa1305260:intron 40.0%
! TTCTCCAAAGGTTTTCCTGT+TGG + chr8_3:68636217-68636236 Msa1305260:intron 40.0%
AGACATGCATGCTGTTCATA+AGG + chr8_3:68636446-68636465 Msa1305260:intron 40.0%
ATGCATGCTGTTCATAAGGT+GGG + chr8_3:68636450-68636469 Msa1305260:intron 40.0%
CAAACTATGAAACGGTCGTT+AGG - chr8_3:68636515-68636534 None:intergenic 40.0%
TGCAGTGATGCTATCATACA+AGG - chr8_3:68636649-68636668 None:intergenic 40.0%
TTTCTTCTAGCAAGTCACCT+TGG - chr8_3:68636808-68636827 None:intergenic 40.0%
CAGCAAGTATAACCACTTTC+TGG - chr8_3:68636890-68636909 None:intergenic 40.0%
TAGTCACACACGTTTGTAGA+CGG - chr8_3:68637036-68637055 None:intergenic 40.0%
ATTTGCATCCAGAATTGCGC+CGG + chr8_3:68633889-68633908 Msa1305260:CDS 45.0%
GAACTGTCAATCGCTTTCAC+AGG + chr8_3:68633997-68634016 Msa1305260:intron 45.0%
AAAACATTAGAGTCCGGGAG+AGG - chr8_3:68634038-68634057 None:intergenic 45.0%
GTATACACTTGGGTCTGACT+TGG + chr8_3:68634441-68634460 Msa1305260:intron 45.0%
TCATAGACCCTAGATCCATG+CGG + chr8_3:68634695-68634714 Msa1305260:intron 45.0%
AGAGACAACAAGACCAAAGG+AGG - chr8_3:68634850-68634869 None:intergenic 45.0%
CGAAGAGACAACAAGACCAA+AGG - chr8_3:68634853-68634872 None:intergenic 45.0%
! TGTTGTCTCTTCGGGTTTCT+AGG + chr8_3:68634860-68634879 Msa1305260:intron 45.0%
!!! CACGTGTAGGTTGGTTTTGT+GGG + chr8_3:68634883-68634902 Msa1305260:intron 45.0%
!!! ACGTGTAGGTTGGTTTTGTG+GGG + chr8_3:68634884-68634903 Msa1305260:intron 45.0%
! CTGGAAAATTTGGTTCCTGC+AGG + chr8_3:68634955-68634974 Msa1305260:intron 45.0%
CCAATTAACTATGTGCCTGC+AGG - chr8_3:68634973-68634992 None:intergenic 45.0%
CCTGCAGGCACATAGTTAAT+TGG + chr8_3:68634970-68634989 Msa1305260:intron 45.0%
AGAAGAAGGCCTTAATTGGC+TGG - chr8_3:68635008-68635027 None:intergenic 45.0%
ATAGAGAACCCGCTATCCTA+TGG - chr8_3:68635187-68635206 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG + chr8_3:68635250-68635269 Msa1305260:intron 45.0%
GTTTGGATTGAGGGTTTAGG+AGG + chr8_3:68635260-68635279 Msa1305260:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG + chr8_3:68635262-68635281 Msa1305260:intron 45.0%
TTGAGGGTTTAGGAGGAGAA+AGG - chr8_3:68635463-68635482 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr8_3:68635260-68635279 None:intergenic 45.0%
CTTAGGGTGTGTTTGGATTG+AGG - chr8_3:68635250-68635269 None:intergenic 45.0%
CTCAATCCAAACACACCCTA+AGG + chr8_3:68635478-68635497 Msa1305260:intron 45.0%
!! TGCATCTAGGGTTAGCTGTT+GGG - chr8_3:68635632-68635651 None:intergenic 45.0%
!! ATGCATCTAGGGTTAGCTGT+TGG - chr8_3:68635633-68635652 None:intergenic 45.0%
GGGAAACACTAAAGAAGAGC+AGG - chr8_3:68635677-68635696 None:intergenic 45.0%
TATGAAGCACGGACACTTCT+CGG + chr8_3:68635784-68635803 Msa1305260:intron 45.0%
! GCATAACTAGTCTGGCATGA+TGG + chr8_3:68635933-68635952 Msa1305260:intron 45.0%
CACGTCCCATCAATTGTATC+TGG - chr8_3:68636426-68636445 None:intergenic 45.0%
CATGCATGCTGTTCATAAGG+TGG + chr8_3:68636449-68636468 Msa1305260:intron 45.0%
TTCTCGAACAGGTCTTCAGA+GGG - chr8_3:68636490-68636509 None:intergenic 45.0%
TTTCTCGAACAGGTCTTCAG+AGG - chr8_3:68636491-68636510 None:intergenic 45.0%
GTCGTTAGGATTTCTCGAAC+AGG - chr8_3:68636501-68636520 None:intergenic 45.0%
AGCTTGGTCTATCAGTTCCA+AGG + chr8_3:68636788-68636807 Msa1305260:CDS 45.0%
! TTACATTGGTGCCCAGAAAG+TGG + chr8_3:68636875-68636894 Msa1305260:three_prime_UTR 45.0%
GATGGATGAGGTGCGAATGT+TGG - chr8_3:68633825-68633844 None:intergenic 50.0%
AAGTGGAGAAGATGTGGCAG+TGG - chr8_3:68633861-68633880 None:intergenic 50.0%
GATGCAAATGATCGGCGAAG+TGG - chr8_3:68633878-68633897 None:intergenic 50.0%
! TCTACTAACTAAGCGCCGCA+TGG - chr8_3:68634713-68634732 None:intergenic 50.0%
AACAAGACCAAAGGAGGCTC+TGG - chr8_3:68634844-68634863 None:intergenic 50.0%
! GTTTCTAGGCACGTGTAGGT+TGG + chr8_3:68634874-68634893 Msa1305260:intron 50.0%
!!! GCACGTGTAGGTTGGTTTTG+TGG + chr8_3:68634882-68634901 Msa1305260:intron 50.0%
!!! CGTGTAGGTTGGTTTTGTGG+GGG + chr8_3:68634885-68634904 Msa1305260:intron 50.0%
TATGCGGGACCAGCCAATTA+AGG + chr8_3:68634996-68635015 Msa1305260:intron 50.0%
TGGCGGCTTCACCAGTTATT+TGG + chr8_3:68635146-68635165 Msa1305260:intron 50.0%
ATTGAGGGTTTAGGAGGGGA+AGG + chr8_3:68635266-68635285 Msa1305260:intron 50.0%
TTGAGGGTTTAGGAGGGGAA+GGG + chr8_3:68635267-68635286 Msa1305260:intron 50.0%
GGTTTAGGAGGAGAAAGGAG+GGG - chr8_3:68635458-68635477 None:intergenic 50.0%
GGGTTTAGGAGGAGAAAGGA+GGG - chr8_3:68635459-68635478 None:intergenic 50.0%
AGGGTTTAGGAGGAGAAAGG+AGG - chr8_3:68635460-68635479 None:intergenic 50.0%
!! GAGAAGTGTCCGTGCTTCAT+AGG - chr8_3:68635785-68635804 None:intergenic 50.0%
!! GGTTGGTTTGGGGATGGATG+AGG - chr8_3:68633837-68633856 None:intergenic 55.0%
!! AGTGGTGGTTGGTTTGGGGA+TGG - chr8_3:68633843-68633862 None:intergenic 55.0%
!! TGGCAGTGGTGGTTGGTTTG+GGG - chr8_3:68633847-68633866 None:intergenic 55.0%
!! GTGGCAGTGGTGGTTGGTTT+GGG - chr8_3:68633848-68633867 None:intergenic 55.0%
! TGTGGCAGTGGTGGTTGGTT+TGG - chr8_3:68633849-68633868 None:intergenic 55.0%
GAAGATGTGGCAGTGGTGGT+TGG - chr8_3:68633854-68633873 None:intergenic 55.0%
TGGAGAAGATGTGGCAGTGG+TGG - chr8_3:68633858-68633877 None:intergenic 55.0%
TCGGCGAAGTGGAGAAGATG+TGG - chr8_3:68633867-68633886 None:intergenic 55.0%
CACGTCTCAGCGTAACTTCG+AGG + chr8_3:68633975-68633994 Msa1305260:intron 55.0%
! CTAAGCGCCGCATGGATCTA+GGG - chr8_3:68634705-68634724 None:intergenic 55.0%
! ACTAAGCGCCGCATGGATCT+AGG - chr8_3:68634706-68634725 None:intergenic 55.0%
GAGTCTTCCAGAGCCTCCTT+TGG + chr8_3:68634834-68634853 Msa1305260:intron 55.0%
! TCGGGTTTCTAGGCACGTGT+AGG + chr8_3:68634870-68634889 Msa1305260:intron 55.0%
TAGGAGGAGAAAGGAGGGGA+GGG - chr8_3:68635454-68635473 None:intergenic 55.0%
TTAGGAGGAGAAAGGAGGGG+AGG - chr8_3:68635455-68635474 None:intergenic 55.0%
GCACGGACACTTCTCGGATA+AGG + chr8_3:68635790-68635809 Msa1305260:intron 55.0%
ACACGACACGTGTCTGACAC+GGG - chr8_3:68635822-68635841 None:intergenic 55.0%
GACACGACACGTGTCTGACA+CGG - chr8_3:68635823-68635842 None:intergenic 55.0%
GCACTATGAAGCACGGACAC+CGG - chr8_3:68635915-68635934 None:intergenic 55.0%
TCCAGAATTGCGCCGGAGCT+TGG + chr8_3:68633896-68633915 Msa1305260:CDS 60.0%
TCCAAGCTCCGGCGCAATTC+TGG - chr8_3:68633900-68633919 None:intergenic 60.0%
GACTGCACGCATCCAAGCTC+CGG - chr8_3:68633911-68633930 None:intergenic 60.0%
GCCACTTCACAGCGCAGTTG+CGG + chr8_3:68633937-68633956 Msa1305260:CDS 60.0%
ACTTCACAGCGCAGTTGCGG+CGG + chr8_3:68633940-68633959 Msa1305260:CDS 60.0%
ACTCTCTGCTGCACCTCTCC+CGG + chr8_3:68634022-68634041 Msa1305260:intron 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr8_3:68635270-68635289 Msa1305260:intron 60.0%
CACGACACGTGTCTGACACG+GGG - chr8_3:68635821-68635840 None:intergenic 60.0%
! GCTGTGTCGGTGTCCATGTC+CGG + chr8_3:68635893-68635912 Msa1305260:intron 60.0%
TGAAGCACGGACACCGGACA+TGG - chr8_3:68635909-68635928 None:intergenic 60.0%
GCCGCAACTGCGCTGTGAAG+TGG - chr8_3:68633941-68633960 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGCG+AGG + chr8_3:68635275-68635294 Msa1305260:intron 65.0%
TAGGAGGGGAAGGGAGGCGA+GGG + chr8_3:68635276-68635295 Msa1305260:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 68633755 68637230 68633755 ID=Msa1305260;Name=Msa1305260
chr8_3 mRNA 68633755 68637230 68633755 ID=Msa1305260-mRNA-1;Parent=Msa1305260;Name=Msa1305260-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0.5|1|0|0|2|383|96
chr8_3 exon 68633755 68633985 68633755 ID=Msa1305260-mRNA-1:exon:21238;Parent=Msa1305260-mRNA-1
chr8_3 exon 68636788 68637230 68636788 ID=Msa1305260-mRNA-1:exon:21239;Parent=Msa1305260-mRNA-1
chr8_3 CDS 68633755 68633985 68633755 ID=Msa1305260-mRNA-1:cds;Parent=Msa1305260-mRNA-1
chr8_3 CDS 68636788 68636847 68636788 ID=Msa1305260-mRNA-1:cds;Parent=Msa1305260-mRNA-1
chr8_3 three_prime_UTR 68636848 68637230 68636848 ID=Msa1305260-mRNA-1:three_prime_utr;Parent=Msa1305260-mRNA-1
Gene Sequence

>Msa1305260

ATGGCTTACGCATGCAGCAGAATCGCTCAAAGAGCATCAATTTCATCCATCAAATCCGCAATTAAATCCAACATTCGCACCTCATCCATCCCCAAACCAACCACCACTGCCACATCTTCTCCACTTCGCCGATCATTTGCATCCAGAATTGCGCCGGAGCTTGGATGCGTGCAGTCGATGTTGCCACTTCACAGCGCAGTTGCGGCGGCGAGAATGACTTCACGTCTCAGCAGCTTGGTCTATCAGTTCCAAGGTGACTTGCTAGAAGAAATATTGAAGAAAGCCTTTTAA
Protein sequence

>Msa1305260

MAYACSRIAQRASISSIKSAIKSNIRTSSIPKPTTTATSSPLRRSFASRIAPELGCVQSMLPLHSAVAAARMTSRLSSLVYQFQGDLLEEILKKAF*