Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246660 | XP_013445609.2 | 90.086 | 817 | 81 | 0 | 1 | 817 | 32 | 848 | 0.0 | 1549 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246660 | sp|O81832|Y4729_ARATH | 49.148 | 822 | 357 | 11 | 9 | 817 | 10 | 783 | 0.0 | 778 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246660 | A0A396GQP4 | 90.086 | 817 | 81 | 0 | 1 | 817 | 32 | 848 | 0.0 | 1549 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0225470 | Msa1246660 | 0.801009 | 1.102246e-48 | -8.615850e-47 |
| Msa0389810 | Msa1246660 | 0.810702 | 1.011029e-50 | -8.615850e-47 |
| Msa0464420 | Msa1246660 | 0.809116 | 2.218448e-50 | -8.615850e-47 |
| Msa0468130 | Msa1246660 | 0.800952 | 1.131864e-48 | -8.615850e-47 |
| Msa0510040 | Msa1246660 | 0.814894 | 1.221216e-51 | -8.615850e-47 |
| Msa0982270 | Msa1246660 | 0.809567 | 1.775568e-50 | -8.615850e-47 |
| Msa1052140 | Msa1246660 | 0.809679 | 1.679938e-50 | -8.615850e-47 |
| Msa1171800 | Msa1246660 | 0.805552 | 1.263526e-49 | -8.615850e-47 |
| Msa1246660 | Msa1246670 | 0.882221 | 1.248953e-70 | -8.615850e-47 |
| Msa1246660 | Msa1246680 | 0.826221 | 3.055183e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246660 | MtrunA17_Chr8g0361261 | 90.086 | 817 | 81 | 0 | 1 | 817 | 32 | 848 | 0.0 | 1549 |
| Msa1246660 | MtrunA17_Chr8g0361301 | 89.963 | 817 | 82 | 0 | 1 | 817 | 1 | 817 | 0.0 | 1540 |
| Msa1246660 | MtrunA17_Chr8g0361251 | 76.781 | 814 | 188 | 1 | 5 | 817 | 4 | 817 | 0.0 | 1316 |
| Msa1246660 | MtrunA17_Chr3g0084411 | 52.734 | 823 | 363 | 10 | 9 | 817 | 10 | 820 | 0.0 | 849 |
| Msa1246660 | MtrunA17_Chr3g0084681 | 51.111 | 810 | 342 | 8 | 13 | 817 | 17 | 777 | 0.0 | 820 |
| Msa1246660 | MtrunA17_Chr3g0084491 | 51.220 | 820 | 369 | 11 | 9 | 817 | 24 | 823 | 0.0 | 817 |
| Msa1246660 | MtrunA17_Chr3g0084421 | 52.484 | 785 | 344 | 10 | 49 | 817 | 12 | 783 | 0.0 | 807 |
| Msa1246660 | MtrunA17_Chr3g0084441 | 50.310 | 807 | 377 | 9 | 24 | 817 | 28 | 823 | 0.0 | 805 |
| Msa1246660 | MtrunA17_Chr3g0084431 | 52.146 | 792 | 349 | 10 | 11 | 795 | 15 | 783 | 0.0 | 803 |
| Msa1246660 | MtrunA17_Chr3g0084451 | 50.868 | 806 | 374 | 9 | 24 | 817 | 28 | 823 | 0.0 | 802 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246660 | AT4G27290.2 | 49.819 | 829 | 387 | 12 | 9 | 817 | 10 | 829 | 0.0 | 801 |
| Msa1246660 | AT4G27290.1 | 49.148 | 822 | 357 | 11 | 9 | 817 | 10 | 783 | 0.0 | 778 |
| Msa1246660 | AT4G27300.1 | 46.477 | 809 | 393 | 15 | 28 | 817 | 28 | 815 | 0.0 | 687 |
| Msa1246660 | AT4G21380.1 | 43.446 | 801 | 412 | 12 | 22 | 792 | 26 | 815 | 0.0 | 672 |
| Msa1246660 | AT1G65790.1 | 42.892 | 823 | 428 | 10 | 27 | 817 | 31 | 843 | 0.0 | 659 |
| Msa1246660 | AT1G65800.1 | 43.357 | 828 | 421 | 14 | 27 | 817 | 31 | 847 | 0.0 | 647 |
| Msa1246660 | AT1G11340.1 | 42.875 | 793 | 427 | 13 | 24 | 796 | 18 | 804 | 0.0 | 642 |
| Msa1246660 | AT1G11410.1 | 42.929 | 799 | 431 | 12 | 20 | 796 | 17 | 812 | 0.0 | 625 |
| Msa1246660 | AT1G11410.4 | 42.929 | 799 | 431 | 12 | 20 | 796 | 24 | 819 | 0.0 | 624 |
| Msa1246660 | AT1G11410.3 | 43.110 | 791 | 425 | 12 | 28 | 796 | 1 | 788 | 0.0 | 622 |
Find 176 sgRNAs with CRISPR-Local
Find 530 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAACGCCATTTGGTCCT+CGG | 0.524564 | 8_2:+38317394 | Msa1246660:CDS |
| AAACCAAAGTCTGGCTATCA+TGG | 0.429147 | 8_2:-38317181 | None:intergenic |
| AAACTAAATATTGGTAGATC+AGG | 0.415406 | 8_2:-38319055 | None:intergenic |
| AAACTTGGGGAAGGTGGATT+TGG | 0.277862 | 8_2:+38319121 | Msa1246660:CDS |
| AAAGCCTGTAAATTTCCTAA+AGG | 0.499864 | 8_2:-38317857 | None:intergenic |
| AAAGTCCCTTCTTCAGAATT+GGG | 0.409992 | 8_2:+38318337 | Msa1246660:CDS |
| AAATCAGATGTGTTTAGTTT+CGG | 0.367177 | 8_2:+38319909 | Msa1246660:CDS |
| AAATTCCTCCAACCCTTGAT+CGG | 0.525326 | 8_2:-38319291 | None:intergenic |
| AACAACCACTTCCTCCATTT+CGG | 0.341467 | 8_2:-38318249 | None:intergenic |
| AACAATGGAATATATCATAT+TGG | 0.449207 | 8_2:+38318038 | Msa1246660:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAGTAGGATAGGAATATAG+TGG | - | chr8_2:38316997-38317016 | None:intergenic | 30.0% | |
| TGATGATAGAATAGTAGGAT+AGG | - | chr8_2:38317007-38317026 | None:intergenic | 30.0% | |
| TTTAGTCCAGGAAATTCAAA+AGG | + | chr8_2:38317233-38317252 | Msa1246660:CDS | 30.0% | |
| AAGGAAGATATTTAGGTATG+TGG | + | chr8_2:38317252-38317271 | Msa1246660:CDS | 30.0% | |
| GCAGTTATGGAATTATTTGA+TGG | - | chr8_2:38317434-38317453 | None:intergenic | 30.0% | |
| TCTGTTAACATTGACATTAG+AGG | + | chr8_2:38317638-38317657 | Msa1246660:CDS | 30.0% | |
| TCCTCAACTAGTTATTATGA+AGG | + | chr8_2:38317664-38317683 | Msa1246660:CDS | 30.0% | |
| CCTCAACTAGTTATTATGAA+GGG | + | chr8_2:38317665-38317684 | Msa1246660:CDS | 30.0% | |
| TCTGTGATAAGATTGAGAAT+AGG | + | chr8_2:38317689-38317708 | Msa1246660:CDS | 30.0% | |
| TTATATAAACTCTCCCCTTT+AGG | + | chr8_2:38317842-38317861 | Msa1246660:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 38316519 | 38320609 | 38316519 | ID=Msa1246660;Name=Msa1246660 |
| chr8_2 | mRNA | 38316519 | 38320609 | 38316519 | ID=Msa1246660-mRNA-1;Parent=Msa1246660;Name=Msa1246660-mRNA-1;_AED=0.15;_eAED=0.15;_QI=240|1|1|1|0.85|0.75|8|219|817 |
| chr8_2 | exon | 38316519 | 38316613 | 38316519 | ID=Msa1246660-mRNA-1:exon:11962;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38316935 | 38318358 | 38316935 | ID=Msa1246660-mRNA-1:exon:11963;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38318629 | 38318745 | 38318629 | ID=Msa1246660-mRNA-1:exon:11964;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38318984 | 38319156 | 38318984 | ID=Msa1246660-mRNA-1:exon:11965;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38319239 | 38319449 | 38319239 | ID=Msa1246660-mRNA-1:exon:11966;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38319545 | 38319782 | 38319545 | ID=Msa1246660-mRNA-1:exon:11967;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38319863 | 38320013 | 38319863 | ID=Msa1246660-mRNA-1:exon:11968;Parent=Msa1246660-mRNA-1 |
| chr8_2 | exon | 38320106 | 38320609 | 38320106 | ID=Msa1246660-mRNA-1:exon:11969;Parent=Msa1246660-mRNA-1 |
| chr8_2 | five_prime_UTR | 38316519 | 38316613 | 38316519 | ID=Msa1246660-mRNA-1:five_prime_utr;Parent=Msa1246660-mRNA-1 |
| chr8_2 | five_prime_UTR | 38316935 | 38317079 | 38316935 | ID=Msa1246660-mRNA-1:five_prime_utr;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38317080 | 38318358 | 38317080 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38318629 | 38318745 | 38318629 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38318984 | 38319156 | 38318984 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38319239 | 38319449 | 38319239 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38319545 | 38319782 | 38319545 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38319863 | 38320013 | 38319863 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | CDS | 38320106 | 38320390 | 38320106 | ID=Msa1246660-mRNA-1:cds;Parent=Msa1246660-mRNA-1 |
| chr8_2 | three_prime_UTR | 38320391 | 38320609 | 38320391 | ID=Msa1246660-mRNA-1:three_prime_utr;Parent=Msa1246660-mRNA-1 |
| Gene Sequence |
>Msa1246660 ATGGTTGATATTAGCTTCAAAAAGTTAATCATTTTGTTCTTTTTATCTTCCAGCTTGAGATCAACCACTTCACTTGACAGTTTAACCTTGAATCAATCTATCCATGATAGCCAGACTTTGGTTTCAGCCAAAGGAACTTTTGAAGTCGGTTTCTTTAGTCCAGGAAATTCAAAAGGAAGATATTTAGGTATGTGGTACAGAAATTTAACCCCTTTAACAGTTGTTTGGGTTGCCAACAGAGAAACACCACTGCACAATAATTTAGGAGTCTTAAAACTCAATGAAAATGGAGTTCTTGTGATTCTCAACGACACAAACAACGCCATTTGGTCCTCGGATGCATCAAACAAACCATCAAATAATTCCATAACTGCACAGCTATTGGACAATGGAAATCTTGTATTGAAAAATTCGTCAAACAATATTTTGTGGCAGACTTTTGATTATCCATGTGATACATTACTACCGGGAATGAAGCTTGGATGGAACTTAGTAACTGGTTTAAACATGTTTGAATCGTCGTGGAAAAGTGCAGATGATCCAGCAAAGGGAATTTATTCTGTTAACATTGACATTAGAGGATATCCTCAACTAGTTATTATGAAGGGATCTGTGATAAGATTGAGAATAGGATCGTGGAACGGTCGTGCTTTTACCGGATATCCAACACAACCATTGAAACAAAAGCAAAGATTTGAATTTGTCATGACTGAAAAAGAAGTGTATCATCGGTACAAAGTTGTTGATAATTCTGTTGTATCTTTATATAAACTCTCCCCTTTAGGAAATTTACAGGCTTTAGCTTGGACAAGTGAAAAAAGCGAAAGGATAGTGATTGAAACGGGCATGGAAGATTCGTGTGATAGTTATGCAATGTGTGGTGCTAATTCTGTATGCAATATGGATGGTAATGTTCCTAAATGTCAATGTTTAAAAGGTTATGTTCCTAAGTTTCCGGAACAATGGAATATATCATATTGGTCTAGTGGTTGTGTTCCAAAAATGAAGATTTTTTGTGGAAACAATAACACAAGTGGTTTCTTGAAATACAGTGGACTGAAATTGCCAGATACGTCTTCTACGTGGTACAATAAAACCATGAACCTTGTGGAATGTCAGAAGGCGTGTACGAACAATTGTTCATGTACGGCTTATACAAATGTGGATATCCGAAATGGAGGAAGTGGTTGTTTACTTTGGTTTGATGATCTAATTGACATGAGAGTATTTTCTCAATGGGGACAAGATCTTTATATCAAAGTCCCTTCTTCAGAATTGGATGATGTTAGTGTTAATAGCAACAAAAACCAGAGGCAGCAGTTGATACGGATCACAGTTGCCGTGATAATTTCAGGATTCCTCACATGTGCTTGCATAGTAATATTTATAAAGAGAGTGGCGCCGAGAGTATATCATCTTGCACCATTTCAATGGAGGAAAAAATATTTTAGGTTGAGGAAAGAAGAGCCTGATCTACCAATATTTAGTTTTCCAATCATTGTTAAAGCAACTGGTAATTTTTCTAGCCGAAATAAACTTGGGGAAGGTGGATTTGGACCAGTTTATAAGGGTACCCTAATAGGTGGGAAAGTGGTAGCCATAAAACGGAACTCAAAAATGTCCGATCAAGGGTTGGAGGAATTTAAAAATGAAGTTTTGTTGATTGCAAAACTTCAGCACCGTAATCTTGTGAAGCTTCTTGGCTGTTGTATTCACAGAGAAGAAAAATTGTTGATCTATGAATACATGCCCAATAGGAGCTTAGACTACTTCATCTTTGATGAAACTAGAAGCAAACTCTTGGACTGGTCGAAGCGTTCCCATATTATTGCTGGCGTTGCTAGAGGACTTCTTTATCTTCACCAAGACTCCAGACTTAGAATAATTCACAGAGATTTAAAGCTAAGCAATATCTTACTAGACGCCCTTATGAATCCAAAAATCTCAGACTTTGGCCTGGCTCGGACATTTTGTGGAGATCAAGTTGAAGCCAAAACAAGGAAATTGGTTGGAACATATGGCTACATGCCTCCAGAGTATGCTGTTCATGGTCGTTACTCCATGAAATCAGATGTGTTTAGTTTCGGAGTAATAGTATTGGAGATAATAAGCGGGAAAAAGATTAAGGTGTTTTATGATCCAGAACACTCTCTCAACCTTCTTGGCCATGCATGGAGACTCTGGATTGATAACATGGCAATGGAACTAGTTGATATCCACTTACTTGAAACAATCAATTATTCTGAAATCTTACGATATATACATGTTGGGTTGTTGTGCGTACAAGAAAAACCGGGAGATAGACCAGACATGTCATTTGTAATTCTTATGTTGAATGGTGAGAAATTATTGTCTCAGCCGAAAGCTCCGGGATTTTATACTGGAAGGGGATTACCTAAAACAATATCGCCAACTTCAAATGAGATGTCACTTACAACTTTTGAGGCAAGATAA |
| Protein sequence |
>Msa1246660 MVDISFKKLIILFFLSSSLRSTTSLDSLTLNQSIHDSQTLVSAKGTFEVGFFSPGNSKGRYLGMWYRNLTPLTVVWVANRETPLHNNLGVLKLNENGVLVILNDTNNAIWSSDASNKPSNNSITAQLLDNGNLVLKNSSNNILWQTFDYPCDTLLPGMKLGWNLVTGLNMFESSWKSADDPAKGIYSVNIDIRGYPQLVIMKGSVIRLRIGSWNGRAFTGYPTQPLKQKQRFEFVMTEKEVYHRYKVVDNSVVSLYKLSPLGNLQALAWTSEKSERIVIETGMEDSCDSYAMCGANSVCNMDGNVPKCQCLKGYVPKFPEQWNISYWSSGCVPKMKIFCGNNNTSGFLKYSGLKLPDTSSTWYNKTMNLVECQKACTNNCSCTAYTNVDIRNGGSGCLLWFDDLIDMRVFSQWGQDLYIKVPSSELDDVSVNSNKNQRQQLIRITVAVIISGFLTCACIVIFIKRVAPRVYHLAPFQWRKKYFRLRKEEPDLPIFSFPIIVKATGNFSSRNKLGEGGFGPVYKGTLIGGKVVAIKRNSKMSDQGLEEFKNEVLLIAKLQHRNLVKLLGCCIHREEKLLIYEYMPNRSLDYFIFDETRSKLLDWSKRSHIIAGVARGLLYLHQDSRLRIIHRDLKLSNILLDALMNPKISDFGLARTFCGDQVEAKTRKLVGTYGYMPPEYAVHGRYSMKSDVFSFGVIVLEIISGKKIKVFYDPEHSLNLLGHAWRLWIDNMAMELVDIHLLETINYSEILRYIHVGLLCVQEKPGDRPDMSFVILMLNGEKLLSQPKAPGFYTGRGLPKTISPTSNEMSLTTFEAR* |
