AlfalfaGEDB | Msa0225470

M. sativa cultivar zhongmu-4 / Msa0225470

KEGG: K17302
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805
PFAM: PF00400.35

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225470	XP_013465536.1	96.815	157	5	0	1	157	148	304	2.90e-108	321

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225470	sp\|Q5VQ78\|COB21_ORYSJ	65.584	154	53	0	1	154	146	299	3.84e-70	230

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225470	A0A072VD91	96.815	157	5	0	1	157	148	304	1.38e-108	321

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0041390	Msa0225470	0.802854	4.605219e-49	-8.615850e-47
Msa0043730	Msa0225470	0.814297	1.655358e-51	-8.615850e-47
Msa0051210	Msa0225470	0.814202	1.736964e-51	-8.615850e-47
Msa0051450	Msa0225470	0.821577	3.749144e-53	-8.615850e-47
Msa0100080	Msa0225470	0.813582	2.379427e-51	-8.615850e-47
Msa0101830	Msa0225470	0.830633	2.630564e-55	-8.615850e-47
Msa0103860	Msa0225470	0.817304	3.534276e-52	-8.615850e-47
Msa0108560	Msa0225470	0.810883	9.238305e-51	-8.615850e-47
Msa0109590	Msa0225470	0.800161	1.641096e-48	-8.615850e-47
Msa0130520	Msa0225470	0.801532	8.612551e-49	-8.615850e-47
Msa0135410	Msa0225470	0.812834	3.472581e-51	-8.615850e-47
Msa0137570	Msa0225470	0.807427	5.082206e-50	-8.615850e-47
Msa0142280	Msa0225470	0.818823	1.603127e-52	-8.615850e-47
Msa0155340	Msa0225470	0.837316	5.586639e-57	-8.615850e-47
Msa0182570	Msa0225470	0.811158	8.051912e-51	-8.615850e-47
Msa0225470	Msa0329700	0.814040	1.886172e-51	-8.615850e-47
Msa0225470	Msa0389810	0.816097	6.589730e-52	-8.615850e-47
Msa0225470	Msa0433530	0.839103	1.935612e-57	-8.615850e-47
Msa0225470	Msa0438040	0.824585	7.453154e-54	-8.615850e-47
Msa0225470	Msa0455590	0.802570	5.268389e-49	-8.615850e-47
Msa0225470	Msa0464420	0.807354	5.266031e-50	-8.615850e-47
Msa0225470	Msa0468130	0.816593	5.104412e-52	-8.615850e-47
Msa0225470	Msa0477260	0.831567	1.551653e-55	-8.615850e-47
Msa0225470	Msa0489610	0.813040	3.129681e-51	-8.615850e-47
Msa0225470	Msa0510040	0.809655	1.699805e-50	-8.615850e-47
Msa0225470	Msa0530650	0.802705	4.942207e-49	-8.615850e-47
Msa0225470	Msa0534410	0.813840	2.087693e-51	-8.615850e-47
Msa0225470	Msa0541040	0.800508	1.394687e-48	-8.615850e-47
Msa0225470	Msa0545290	0.814610	1.411128e-51	-8.615850e-47
Msa0225470	Msa0587190	0.815136	1.078778e-51	-8.615850e-47
Msa0225470	Msa0590650	0.802114	6.540852e-49	-8.615850e-47
Msa0225470	Msa0597000	0.800695	1.277304e-48	-8.615850e-47
Msa0225470	Msa0600890	0.816989	4.160821e-52	-8.615850e-47
Msa0225470	Msa0615340	0.802923	4.456039e-49	-8.615850e-47
Msa0225470	Msa0626990	0.801073	1.069241e-48	-8.615850e-47
Msa0225470	Msa0627350	0.807921	3.990838e-50	-8.615850e-47
Msa0225470	Msa0630950	0.822892	1.856991e-53	-8.615850e-47
Msa0225470	Msa0660300	0.822470	2.328723e-53	-8.615850e-47
Msa0225470	Msa0679550	0.822708	2.049798e-53	-8.615850e-47
Msa0225470	Msa0679810	0.811397	7.145708e-51	-8.615850e-47
Msa0225470	Msa0696420	0.807575	4.726319e-50	-8.615850e-47
Msa0225470	Msa0715410	0.810950	8.931350e-51	-8.615850e-47
Msa0225470	Msa0731180	0.831581	1.539603e-55	-8.615850e-47
Msa0225470	Msa0742150	0.811249	7.695836e-51	-8.615850e-47
Msa0225470	Msa0771580	0.840259	9.686268e-58	-8.615850e-47
Msa0225470	Msa0786070	0.807243	5.559501e-50	-8.615850e-47
Msa0225470	Msa0876360	0.805354	1.390418e-49	-8.615850e-47
Msa0225470	Msa0932100	0.831116	2.003422e-55	-8.615850e-47
Msa0225470	Msa0932190	0.817273	3.591453e-52	-8.615850e-47
Msa0225470	Msa0970230	0.835211	1.915050e-56	-8.615850e-47
Msa0225470	Msa0982270	0.814905	1.214321e-51	-8.615850e-47
Msa0225470	Msa0996170	0.801356	9.359597e-49	-8.615850e-47
Msa0225470	Msa1023300	0.829767	4.280765e-55	-8.615850e-47
Msa0225470	Msa1027290	0.802297	5.997673e-49	-8.615850e-47
Msa0225470	Msa1052140	0.807093	5.981857e-50	-8.615850e-47
Msa0225470	Msa1087990	0.835733	1.412644e-56	-8.615850e-47
Msa0225470	Msa1115280	0.801844	7.434335e-49	-8.615850e-47
Msa0225470	Msa1150710	0.803816	2.910584e-49	-8.615850e-47
Msa0225470	Msa1150800	0.820742	5.841428e-53	-8.615850e-47
Msa0225470	Msa1150810	0.843427	1.410949e-58	-8.615850e-47
Msa0225470	Msa1224200	0.810134	1.340732e-50	-8.615850e-47
Msa0225470	Msa1246660	0.801009	1.102246e-48	-8.615850e-47
Msa0225470	Msa1285890	0.814878	1.231020e-51	-8.615850e-47
Msa0225470	Msa1287680	0.807699	4.449516e-50	-8.615850e-47
Msa0225470	Msa1306750	0.838864	2.231710e-57	-8.615850e-47
Msa0225470	Msa1307740	0.821703	3.507517e-53	-8.615850e-47
Msa0225470	Msa1314770	0.808950	2.407140e-50	-8.615850e-47
Msa0225470	Msa1348600	0.828006	1.142324e-54	-8.615850e-47
Msa0225470	Msa1348850	0.809724	1.642703e-50	-8.615850e-47
Msa0225470	Msa1377080	0.824043	9.992478e-54	-8.615850e-47
Msa0225470	Msa1396830	0.826808	2.213792e-54	-8.615850e-47
Msa0225470	Msa1437720	0.830133	3.485761e-55	-8.615850e-47
Msa0225470	Msa1448630	0.811005	8.690920e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225470	MtrunA17_Chr2g0331961	96.815	157	5	0	1	157	148	304	2.67e-112	321
Msa0225470	MtrunA17_Chr4g0004241	66.234	154	52	0	1	154	146	299	4.57e-71	230
Msa0225470	MtrunA17_Chr4g0004241	29.091	110	75	2	3	112	21	127	1.30e-12	64.7
Msa0225470	MtrunA17_Chr2g0331981	65.584	154	53	0	1	154	146	299	6.46e-70	226
Msa0225470	MtrunA17_Chr2g0331981	29.091	110	75	2	3	112	21	127	1.71e-12	64.3
Msa0225470	MtrunA17_Chr3g0136971	31.875	160	95	3	3	162	467	612	8.99e-19	82.4
Msa0225470	MtrunA17_Chr1g0185501	33.571	140	91	1	9	148	38	175	9.96e-19	81.3
Msa0225470	MtrunA17_Chr1g0185501	35.354	99	61	2	16	111	214	312	1.31e-13	67.0
Msa0225470	MtrunA17_Chr1g0185501	32.456	114	75	1	9	122	80	191	7.81e-12	62.0
Msa0225470	MtrunA17_Chr6g0448921	36.792	106	65	1	13	118	116	219	1.86e-17	78.6
Msa0225470	MtrunA17_Chr6g0448921	32.381	105	69	1	14	118	75	177	4.34e-14	68.9
Msa0225470	MtrunA17_Chr1g0197551	29.444	180	95	5	1	151	139	315	1.89e-16	75.9
Msa0225470	MtrunA17_Chr1g0197551	31.818	110	72	2	3	112	57	163	4.65e-15	71.6
Msa0225470	MtrunA17_Chr4g0035611	28.333	180	97	4	1	151	139	315	3.06e-16	75.1
Msa0225470	MtrunA17_Chr4g0035611	33.636	110	70	2	3	112	57	163	6.00e-16	74.3
Msa0225470	MtrunA17_Chr2g0295791	35.185	108	68	1	15	122	229	334	1.17e-15	73.2
Msa0225470	MtrunA17_Chr2g0295791	33.962	106	66	2	9	112	179	282	2.19e-15	72.4
Msa0225470	MtrunA17_Chr4g0067151	36.697	109	58	2	13	112	42	148	4.49e-15	71.2
Msa0225470	MtrunA17_Chr4g0067151	33.333	114	74	1	9	122	89	200	2.50e-13	66.2
Msa0225470	MtrunA17_Chr4g0067151	30.357	112	74	3	3	111	211	321	3.71e-11	60.1
Msa0225470	MtrunA17_Chr4g0059031	32.353	136	81	4	9	133	111	246	2.16e-14	69.7
Msa0225470	MtrunA17_Chr4g0059031	27.465	142	100	2	3	144	506	644	4.16e-11	60.5
Msa0225470	MtrunA17_Chr5g0401671	32.394	142	85	2	12	144	402	541	5.08e-14	68.6
Msa0225470	MtrunA17_Chr2g0281451	27.027	148	106	1	13	160	115	260	9.90e-14	67.8
Msa0225470	MtrunA17_Chr2g0281451	26.364	110	79	1	15	124	75	182	1.76e-11	61.6
Msa0225470	MtrunA17_Chr7g0246741	38.679	106	62	2	12	117	342	444	1.23e-13	67.8
Msa0225470	MtrunA17_Chr4g0070791	28.889	135	94	2	1	135	162	294	2.44e-13	66.2
Msa0225470	MtrunA17_Chr5g0401661	31.818	154	93	3	12	155	492	643	2.50e-13	66.6
Msa0225470	MtrunA17_Chr2g0285951	33.673	98	63	1	15	112	47	142	2.02e-12	63.2
Msa0225470	MtrunA17_Chr8g0392701	31.690	142	91	3	15	152	80	219	2.40e-12	63.5
Msa0225470	MtrunA17_Chr3g0093681	25.325	154	103	3	9	161	409	551	2.81e-12	63.5
Msa0225470	MtrunA17_Chr1g0172501	25.325	154	103	3	9	161	409	551	4.33e-12	63.2
Msa0225470	MtrunA17_Chr1g0172501	30.435	115	78	2	1	115	444	556	7.18e-11	59.7
Msa0225470	MtrunA17_Chr1g0176241	34.545	110	69	2	2	111	375	481	4.42e-12	63.2
Msa0225470	MtrunA17_Chr1g0176241	34.746	118	68	4	1	112	123	237	1.79e-11	61.2
Msa0225470	MtrunA17_Chr1g0176241	30.147	136	92	3	10	144	339	472	3.68e-11	60.5
Msa0225470	MtrunA17_Chr4g0053651	30.070	143	89	2	11	144	384	524	5.17e-12	62.8
Msa0225470	MtrunA17_Chr4g0069701	30.075	133	90	2	1	133	544	673	5.59e-12	62.8
Msa0225470	MtrunA17_Chr8g0390861	34.821	112	66	3	8	117	879	985	6.81e-12	62.8
Msa0225470	MtrunA17_Chr5g0405851	26.452	155	102	3	2	156	258	400	8.20e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225470	AT1G52360.3	66.883	154	51	0	1	154	146	299	7.13e-71	229
Msa0225470	AT1G52360.3	28.182	110	76	2	3	112	21	127	4.62e-12	63.5
Msa0225470	AT1G52360.4	66.883	154	51	0	1	154	146	299	8.62e-71	229
Msa0225470	AT1G52360.4	28.182	110	76	2	3	112	21	127	5.24e-12	63.5
Msa0225470	AT1G52360.1	66.883	154	51	0	1	154	146	299	8.62e-71	229
Msa0225470	AT1G52360.1	28.182	110	76	2	3	112	21	127	5.24e-12	63.5
Msa0225470	AT1G79990.2	66.883	154	51	0	1	154	146	299	1.04e-70	229
Msa0225470	AT1G79990.2	27.273	110	77	2	3	112	21	127	8.70e-12	62.8
Msa0225470	AT1G79990.1	66.883	154	51	0	1	154	146	299	1.40e-70	229
Msa0225470	AT1G79990.1	27.273	110	77	2	3	112	21	127	8.87e-12	62.8
Msa0225470	AT1G52360.2	66.883	154	51	0	1	154	190	343	1.76e-70	229
Msa0225470	AT1G52360.2	28.182	110	76	2	3	112	65	171	4.56e-12	63.5
Msa0225470	AT3G15980.1	66.234	154	52	0	1	154	146	299	3.33e-70	228
Msa0225470	AT3G15980.1	28.182	110	76	2	3	112	21	127	2.65e-12	64.3
Msa0225470	AT3G15980.7	66.234	154	52	0	1	154	146	299	3.63e-70	228
Msa0225470	AT3G15980.7	28.182	110	76	2	3	112	21	127	2.67e-12	64.3
Msa0225470	AT3G15980.3	66.234	154	52	0	1	154	146	299	3.81e-70	228
Msa0225470	AT3G15980.3	28.182	110	76	2	3	112	21	127	3.03e-12	63.9
Msa0225470	AT3G15980.6	66.234	154	52	0	1	154	146	299	3.81e-70	228
Msa0225470	AT3G15980.6	28.182	110	76	2	3	112	21	127	3.03e-12	63.9
Msa0225470	AT3G15980.2	66.234	154	52	0	1	154	146	299	3.81e-70	228
Msa0225470	AT3G15980.2	28.182	110	76	2	3	112	21	127	3.03e-12	63.9
Msa0225470	AT3G15980.4	66.234	154	52	0	1	154	146	299	3.81e-70	228
Msa0225470	AT3G15980.4	28.182	110	76	2	3	112	21	127	3.03e-12	63.9
Msa0225470	AT3G15980.5	66.234	154	52	0	1	154	146	299	4.28e-70	228
Msa0225470	AT3G15980.5	28.182	110	76	2	3	112	21	127	2.65e-12	64.3
Msa0225470	AT5G25150.2	34.000	150	86	2	13	162	475	611	6.75e-19	83.2
Msa0225470	AT5G25150.1	34.000	150	86	2	13	162	475	611	6.95e-19	83.2
Msa0225470	AT5G08390.1	35.849	106	66	1	13	118	116	219	7.80e-17	77.4
Msa0225470	AT5G08390.1	28.244	131	92	1	14	144	75	203	2.20e-12	64.3
Msa0225470	AT2G21390.1	28.889	180	96	5	1	151	139	315	8.45e-17	77.0
Msa0225470	AT2G21390.1	32.727	110	71	2	3	112	57	163	7.89e-16	74.3
Msa0225470	AT2G21390.1	34.545	110	69	2	3	112	15	121	2.06e-15	73.2
Msa0225470	AT5G23430.1	35.849	106	66	1	13	118	116	219	1.21e-16	76.6
Msa0225470	AT5G23430.1	29.524	105	72	1	14	118	75	177	5.03e-12	63.5
Msa0225470	AT5G23430.3	35.849	106	66	1	13	118	116	219	1.23e-16	76.6
Msa0225470	AT5G23430.3	29.524	105	72	1	14	118	75	177	5.12e-12	63.5
Msa0225470	AT5G23430.2	35.849	106	66	1	13	118	116	219	1.23e-16	76.6
Msa0225470	AT5G23430.2	29.524	105	72	1	14	118	75	177	5.12e-12	63.5
Msa0225470	AT1G62020.1	27.778	180	98	4	1	151	139	315	2.44e-16	75.9
Msa0225470	AT1G62020.1	34.545	110	69	2	3	112	15	121	1.40e-15	73.6
Msa0225470	AT1G62020.1	32.174	115	75	2	3	117	57	168	2.43e-15	73.2
Msa0225470	AT3G49660.1	30.882	136	87	2	9	139	35	168	1.87e-15	72.4
Msa0225470	AT3G49660.1	33.636	110	71	2	3	111	204	312	8.13e-15	70.9
Msa0225470	AT3G49660.1	32.500	120	78	2	3	122	77	193	2.04e-12	63.9
Msa0225470	AT3G16650.1	30.973	113	75	2	3	115	176	285	3.32e-15	72.4
Msa0225470	AT3G16650.1	34.694	98	61	2	15	112	229	323	2.02e-11	61.6
Msa0225470	AT4G15900.1	30.973	113	75	2	3	115	182	291	3.90e-15	72.4
Msa0225470	AT4G15900.1	33.333	108	70	1	15	122	235	340	9.81e-14	68.2
Msa0225470	AT1G11160.1	31.897	116	76	2	3	118	64	176	1.06e-14	71.2
Msa0225470	AT1G11160.1	27.027	148	106	1	13	160	115	260	9.59e-14	68.6
Msa0225470	AT1G11160.3	31.897	116	76	2	3	118	22	134	1.08e-14	71.2
Msa0225470	AT1G11160.3	27.027	148	106	1	13	160	73	218	9.63e-14	68.6
Msa0225470	AT4G02730.1	36.111	108	66	2	9	115	54	159	2.41e-14	69.7
Msa0225470	AT1G11160.4	31.897	116	76	2	3	118	64	176	4.52e-14	69.3
Msa0225470	AT1G11160.4	27.027	148	106	1	13	160	115	260	1.80e-13	67.8
Msa0225470	AT1G11160.2	31.897	116	76	2	3	118	64	176	4.57e-14	69.3
Msa0225470	AT1G11160.2	27.027	148	106	1	13	160	115	260	1.74e-13	67.8
Msa0225470	AT4G29830.1	30.370	135	92	2	1	135	162	294	8.86e-14	67.8
Msa0225470	AT1G61210.2	29.508	122	83	2	3	124	64	182	1.00e-13	68.2
Msa0225470	AT1G61210.2	27.703	148	105	1	13	160	115	260	1.77e-12	64.7
Msa0225470	AT1G61210.1	29.508	122	83	2	3	124	64	182	1.02e-13	68.2
Msa0225470	AT1G61210.1	27.703	148	105	1	13	160	115	260	1.85e-12	64.7
Msa0225470	AT1G61210.3	28.169	142	99	2	3	144	64	202	1.32e-13	68.2
Msa0225470	AT1G61210.3	27.703	148	105	1	13	160	115	260	1.59e-12	64.7
Msa0225470	AT5G16750.1	30.702	114	75	1	13	122	117	230	6.55e-13	65.9
Msa0225470	AT1G48630.1	34.821	112	67	3	15	122	80	189	1.31e-12	64.7
Msa0225470	AT1G15440.1	38.679	106	62	2	12	117	362	464	1.32e-12	65.1
Msa0225470	AT1G15440.2	38.679	106	62	2	12	117	322	424	1.48e-12	65.1
Msa0225470	AT1G73720.1	36.111	108	67	1	8	115	273	378	2.74e-12	63.9
Msa0225470	AT5G52820.1	33.036	112	72	2	2	113	365	473	5.25e-12	63.2
Msa0225470	AT5G67320.1	30.986	142	87	2	12	144	420	559	5.79e-12	63.2
Msa0225470	AT3G18130.1	33.929	112	68	3	15	122	80	189	7.58e-12	62.4
Msa0225470	AT1G18080.1	30.556	144	91	4	15	152	80	220	6.30e-11	59.7
Msa0225470	AT1G71840.1	29.915	117	77	3	3	117	290	403	6.59e-11	60.1
Msa0225470	AT1G49040.1	33.636	110	66	3	8	115	865	969	9.26e-11	59.7
Msa0225470	AT1G49040.3	33.636	110	66	3	8	115	865	969	9.59e-11	59.7
Msa0225470	AT2G47990.1	33.019	106	69	2	9	113	104	208	9.77e-11	59.7

Find 30 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAAGCAGTTATTATTAT+AGG	0.174991	2_1:-75595012	None:intergenic
TGTCCTTCAAAGGTAAAATT+TGG	0.208590	2_1:-75594732	None:intergenic
ATCCCTTGATGGCACTTTAA+AGG	0.246430	2_1:+75594520	Msa0225470:CDS
AGGATTCGACTGTTAAGATT+TGG	0.281018	2_1:+75595034	Msa0225470:CDS
ACTTTGGTCTTGAGAGAGTA+TGG	0.306267	2_1:+75595263	Msa0225470:CDS
TTTGCCAGTGCATCCCTTGA+TGG	0.337278	2_1:+75594509	Msa0225470:CDS
ACTTTGTGCAGACTTGCTTT+TGG	0.356628	2_1:+75595401	Msa0225470:intron
GGATTCGACTGTTAAGATTT+GGG	0.363840	2_1:+75595035	Msa0225470:CDS
CTTCAAAACACATTGGACTT+TGG	0.391193	2_1:+75595247	Msa0225470:CDS
GATGCACTGGCAAAGGTAGA+TGG	0.420841	2_1:-75594500	None:intergenic
AACTGTGTACAAACACTTGA+AGG	0.421132	2_1:+75594946	Msa0225470:CDS
GTTATTATTATAGGTATCTC+AGG	0.444378	2_1:-75595003	None:intergenic
GGCAAAGGTAGATGGATCCT+TGG	0.459900	2_1:-75594492	None:intergenic
CTTGAGAGAGTATGGAGTAT+TGG	0.472251	2_1:+75595271	Msa0225470:CDS
TTTGGGATGCTGTCACCTAT+AGG	0.482117	2_1:+75595052	Msa0225470:CDS
TCATTTAATCTTGTTTCACT+TGG	0.487565	2_1:-75595469	None:intergenic
AAAGGTAAAATTTGGAGTTG+AGG	0.495430	2_1:-75594724	None:intergenic
TATAATAATAACTGCTTCAG+AGG	0.502357	2_1:+75595014	Msa0225470:CDS
TTGTGACAAAGGATTTATCA+TGG	0.502790	2_1:+75595424	Msa0225470:CDS
GCAAAGGTAGATGGATCCTT+GGG	0.508898	2_1:-75594491	None:intergenic
ATACCTTTAAAGTGCCATCA+AGG	0.516706	2_1:-75594523	None:intergenic
ACCTTTGAAGGACACTTGAA+AGG	0.527737	2_1:+75594741	Msa0225470:CDS
ATCAAGGGATGCACTGGCAA+AGG	0.534501	2_1:-75594507	None:intergenic
TCCTTTCAAGTGTCCTTCAA+AGG	0.537839	2_1:-75594742	None:intergenic
AGTGCCATCAAGGGATGCAC+TGG	0.540108	2_1:-75594513	None:intergenic
TTCTGATGATTACACTGCCA+AGG	0.549721	2_1:+75594818	Msa0225470:CDS
TACCTTTAAAGTGCCATCAA+GGG	0.594524	2_1:-75594522	None:intergenic
TTGCAGGCTTCAAAACACAT+TGG	0.596689	2_1:+75595240	Msa0225470:intron
GCAAGTGGCATTTAATCCCA+AGG	0.613075	2_1:+75594475	Msa0225470:CDS
CTCACATTATGTGATGCAAG+TGG	0.679660	2_1:+75594460	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATAGAATTATATATTTCAA+AGG	-	chr2_1:75594880-75594899	None:intergenic	15.0%
!!	GATAGAATTATATATTTCAA+AGG	-	chr2_1:75594880-75594899	None:intergenic	15.0%
!!	AATCTACAAAAAGAGTAAAT+TGG	-	chr2_1:75594691-75594710	None:intergenic	20.0%
!!!	ATTTACTCTTTTTGTAGATT+TGG	+	chr2_1:75594691-75594710	Msa0225470:intron	20.0%
!!	TATATATTTCAAAGGCTAAA+AGG	-	chr2_1:75594872-75594891	None:intergenic	20.0%
!!	TTGAAATGTGAATTTAGTAT+AGG	-	chr2_1:75594902-75594921	None:intergenic	20.0%
!!	TGAAAATATGAAATACCTAT+AGG	-	chr2_1:75595070-75595089	None:intergenic	20.0%
!!	GTATTTAATTAAATTTCTGC+TGG	+	chr2_1:75595187-75595206	Msa0225470:intron	20.0%
!!	AATCTACAAAAAGAGTAAAT+TGG	-	chr2_1:75594691-75594710	None:intergenic	20.0%
!!!	ATTTACTCTTTTTGTAGATT+TGG	+	chr2_1:75594691-75594710	Msa0225470:intron	20.0%
!!	TATATATTTCAAAGGCTAAA+AGG	-	chr2_1:75594872-75594891	None:intergenic	20.0%
!!	TTGAAATGTGAATTTAGTAT+AGG	-	chr2_1:75594902-75594921	None:intergenic	20.0%
!!	TGAAAATATGAAATACCTAT+AGG	-	chr2_1:75595070-75595089	None:intergenic	20.0%
!!	GTATTTAATTAAATTTCTGC+TGG	+	chr2_1:75595187-75595206	Msa0225470:intron	20.0%
!!	GATAAGCAATATCTTTTAAG+TGG	+	chr2_1:75594795-75594814	Msa0225470:CDS	25.0%
!	TACTAAATTCACATTTCAAC+AGG	+	chr2_1:75594903-75594922	Msa0225470:intron	25.0%
!	GTTATTATTATAGGTATCTC+AGG	-	chr2_1:75595006-75595025	None:intergenic	25.0%
!!	TCTGAAGCAGTTATTATTAT+AGG	-	chr2_1:75595015-75595034	None:intergenic	25.0%
!	TATAATAATAACTGCTTCAG+AGG	+	chr2_1:75595014-75595033	Msa0225470:CDS	25.0%
!	CAATATGTTGTCAATTAATG+AGG	-	chr2_1:75595379-75595398	None:intergenic	25.0%
!!	GATAAGCAATATCTTTTAAG+TGG	+	chr2_1:75594795-75594814	Msa0225470:CDS	25.0%
!	TACTAAATTCACATTTCAAC+AGG	+	chr2_1:75594903-75594922	Msa0225470:intron	25.0%
!	GTTATTATTATAGGTATCTC+AGG	-	chr2_1:75595006-75595025	None:intergenic	25.0%
!!	TCTGAAGCAGTTATTATTAT+AGG	-	chr2_1:75595015-75595034	None:intergenic	25.0%
!	TATAATAATAACTGCTTCAG+AGG	+	chr2_1:75595014-75595033	Msa0225470:CDS	25.0%
!	CAATATGTTGTCAATTAATG+AGG	-	chr2_1:75595379-75595398	None:intergenic	25.0%
	GAAATATGTAGTAAGAAGCA+GGG	-	chr2_1:75594644-75594663	None:intergenic	30.0%
	TGAAATATGTAGTAAGAAGC+AGG	-	chr2_1:75594645-75594664	None:intergenic	30.0%
!	AAAGGTAAAATTTGGAGTTG+AGG	-	chr2_1:75594727-75594746	None:intergenic	30.0%
!	ACTCCAAATTTTACCTTTGA+AGG	+	chr2_1:75594729-75594748	Msa0225470:CDS	30.0%
	TGTCCTTCAAAGGTAAAATT+TGG	-	chr2_1:75594735-75594754	None:intergenic	30.0%
	AATTCACATTTCAACAGGTA+TGG	+	chr2_1:75594908-75594927	Msa0225470:intron	30.0%
	ATTCACATTTCAACAGGTAT+GGG	+	chr2_1:75594909-75594928	Msa0225470:intron	30.0%
	TTGTGACAAAGGATTTATCA+TGG	+	chr2_1:75595424-75595443	Msa0225470:CDS	30.0%
	GAAATATGTAGTAAGAAGCA+GGG	-	chr2_1:75594644-75594663	None:intergenic	30.0%
	TGAAATATGTAGTAAGAAGC+AGG	-	chr2_1:75594645-75594664	None:intergenic	30.0%
!	AAAGGTAAAATTTGGAGTTG+AGG	-	chr2_1:75594727-75594746	None:intergenic	30.0%
!	ACTCCAAATTTTACCTTTGA+AGG	+	chr2_1:75594729-75594748	Msa0225470:CDS	30.0%
	TGTCCTTCAAAGGTAAAATT+TGG	-	chr2_1:75594735-75594754	None:intergenic	30.0%
	AATTCACATTTCAACAGGTA+TGG	+	chr2_1:75594908-75594927	Msa0225470:intron	30.0%
	ATTCACATTTCAACAGGTAT+GGG	+	chr2_1:75594909-75594928	Msa0225470:intron	30.0%
	TTGTGACAAAGGATTTATCA+TGG	+	chr2_1:75595424-75595443	Msa0225470:CDS	30.0%
!!	TACCTTTAAAGTGCCATCAA+GGG	-	chr2_1:75594525-75594544	None:intergenic	35.0%
!!	ATACCTTTAAAGTGCCATCA+AGG	-	chr2_1:75594526-75594545	None:intergenic	35.0%
	ACAATAATATCGAGGAGTTG+AGG	-	chr2_1:75594562-75594581	None:intergenic	35.0%
	TCAACTCCTCGATATTATTG+TGG	+	chr2_1:75594561-75594580	Msa0225470:intron	35.0%
	AGTAACTGATATGCAAACCT+TGG	-	chr2_1:75594838-75594857	None:intergenic	35.0%
	AACTGTGTACAAACACTTGA+AGG	+	chr2_1:75594946-75594965	Msa0225470:CDS	35.0%
	AGGATTCGACTGTTAAGATT+TGG	+	chr2_1:75595034-75595053	Msa0225470:CDS	35.0%
	GGATTCGACTGTTAAGATTT+GGG	+	chr2_1:75595035-75595054	Msa0225470:CDS	35.0%
	GCTAATGAAAGTTTCTCAGT+AGG	+	chr2_1:75595141-75595160	Msa0225470:intron	35.0%
	CAAATGCTACTAACTATTGC+AGG	+	chr2_1:75595224-75595243	Msa0225470:intron	35.0%
	CTTCAAAACACATTGGACTT+TGG	+	chr2_1:75595247-75595266	Msa0225470:CDS	35.0%
!!	TGGAGTATTGGCTACAAAAA+AGG	+	chr2_1:75595283-75595302	Msa0225470:CDS	35.0%
!!	TACCTTTAAAGTGCCATCAA+GGG	-	chr2_1:75594525-75594544	None:intergenic	35.0%
!!	ATACCTTTAAAGTGCCATCA+AGG	-	chr2_1:75594526-75594545	None:intergenic	35.0%
	ACAATAATATCGAGGAGTTG+AGG	-	chr2_1:75594562-75594581	None:intergenic	35.0%
	TCAACTCCTCGATATTATTG+TGG	+	chr2_1:75594561-75594580	Msa0225470:intron	35.0%
	AGTAACTGATATGCAAACCT+TGG	-	chr2_1:75594838-75594857	None:intergenic	35.0%
	AACTGTGTACAAACACTTGA+AGG	+	chr2_1:75594946-75594965	Msa0225470:CDS	35.0%
	AGGATTCGACTGTTAAGATT+TGG	+	chr2_1:75595034-75595053	Msa0225470:CDS	35.0%
	GGATTCGACTGTTAAGATTT+GGG	+	chr2_1:75595035-75595054	Msa0225470:CDS	35.0%
	GCTAATGAAAGTTTCTCAGT+AGG	+	chr2_1:75595141-75595160	Msa0225470:intron	35.0%
	CAAATGCTACTAACTATTGC+AGG	+	chr2_1:75595224-75595243	Msa0225470:intron	35.0%
	CTTCAAAACACATTGGACTT+TGG	+	chr2_1:75595247-75595266	Msa0225470:CDS	35.0%
!!	TGGAGTATTGGCTACAAAAA+AGG	+	chr2_1:75595283-75595302	Msa0225470:CDS	35.0%
!	ATCCCTTGATGGCACTTTAA+AGG	+	chr2_1:75594520-75594539	Msa0225470:CDS	40.0%
!	ATCGAGGAGTTGAGGATTTT+CGG	-	chr2_1:75594554-75594573	None:intergenic	40.0%
	GACACTCCACAATAATATCG+AGG	-	chr2_1:75594570-75594589	None:intergenic	40.0%
!	ACCTTTGAAGGACACTTGAA+AGG	+	chr2_1:75594741-75594760	Msa0225470:CDS	40.0%
!	TCCTTTCAAGTGTCCTTCAA+AGG	-	chr2_1:75594745-75594764	None:intergenic	40.0%
	TTCTGATGATTACACTGCCA+AGG	+	chr2_1:75594818-75594837	Msa0225470:CDS	40.0%
	TTGCAGGCTTCAAAACACAT+TGG	+	chr2_1:75595240-75595259	Msa0225470:intron	40.0%
!!	ACTTTGGTCTTGAGAGAGTA+TGG	+	chr2_1:75595263-75595282	Msa0225470:CDS	40.0%
!	CTTGAGAGAGTATGGAGTAT+TGG	+	chr2_1:75595271-75595290	Msa0225470:CDS	40.0%
!!	ACTTTGTGCAGACTTGCTTT+TGG	+	chr2_1:75595401-75595420	Msa0225470:intron	40.0%
!!	CTTGCTTTTGGTTGTGACAA+AGG	+	chr2_1:75595413-75595432	Msa0225470:CDS	40.0%
!	ATCCCTTGATGGCACTTTAA+AGG	+	chr2_1:75594520-75594539	Msa0225470:CDS	40.0%
!	ATCGAGGAGTTGAGGATTTT+CGG	-	chr2_1:75594554-75594573	None:intergenic	40.0%
	GACACTCCACAATAATATCG+AGG	-	chr2_1:75594570-75594589	None:intergenic	40.0%
!	ACCTTTGAAGGACACTTGAA+AGG	+	chr2_1:75594741-75594760	Msa0225470:CDS	40.0%
!	TCCTTTCAAGTGTCCTTCAA+AGG	-	chr2_1:75594745-75594764	None:intergenic	40.0%
	TTCTGATGATTACACTGCCA+AGG	+	chr2_1:75594818-75594837	Msa0225470:CDS	40.0%
	TTGCAGGCTTCAAAACACAT+TGG	+	chr2_1:75595240-75595259	Msa0225470:intron	40.0%
!!	ACTTTGGTCTTGAGAGAGTA+TGG	+	chr2_1:75595263-75595282	Msa0225470:CDS	40.0%
!	CTTGAGAGAGTATGGAGTAT+TGG	+	chr2_1:75595271-75595290	Msa0225470:CDS	40.0%
!!	ACTTTGTGCAGACTTGCTTT+TGG	+	chr2_1:75595401-75595420	Msa0225470:intron	40.0%
!!	CTTGCTTTTGGTTGTGACAA+AGG	+	chr2_1:75595413-75595432	Msa0225470:CDS	40.0%
	GCAAGTGGCATTTAATCCCA+AGG	+	chr2_1:75594475-75594494	Msa0225470:CDS	45.0%
	GCAAAGGTAGATGGATCCTT+GGG	-	chr2_1:75594494-75594513	None:intergenic	45.0%
!	TTTGGGATGCTGTCACCTAT+AGG	+	chr2_1:75595052-75595071	Msa0225470:CDS	45.0%
	GCAAGTGGCATTTAATCCCA+AGG	+	chr2_1:75594475-75594494	Msa0225470:CDS	45.0%
	GCAAAGGTAGATGGATCCTT+GGG	-	chr2_1:75594494-75594513	None:intergenic	45.0%
!	TTTGGGATGCTGTCACCTAT+AGG	+	chr2_1:75595052-75595071	Msa0225470:CDS	45.0%
	GGCAAAGGTAGATGGATCCT+TGG	-	chr2_1:75594495-75594514	None:intergenic	50.0%
!	GATGCACTGGCAAAGGTAGA+TGG	-	chr2_1:75594503-75594522	None:intergenic	50.0%
!	ATCAAGGGATGCACTGGCAA+AGG	-	chr2_1:75594510-75594529	None:intergenic	50.0%
	TTTGCCAGTGCATCCCTTGA+TGG	+	chr2_1:75594509-75594528	Msa0225470:CDS	50.0%
	GGCAAAGGTAGATGGATCCT+TGG	-	chr2_1:75594495-75594514	None:intergenic	50.0%
!	GATGCACTGGCAAAGGTAGA+TGG	-	chr2_1:75594503-75594522	None:intergenic	50.0%
!	ATCAAGGGATGCACTGGCAA+AGG	-	chr2_1:75594510-75594529	None:intergenic	50.0%
	TTTGCCAGTGCATCCCTTGA+TGG	+	chr2_1:75594509-75594528	Msa0225470:CDS	50.0%
	AGTGCCATCAAGGGATGCAC+TGG	-	chr2_1:75594516-75594535	None:intergenic	55.0%
	AGTGCCATCAAGGGATGCAC+TGG	-	chr2_1:75594516-75594535	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	75594473	75595487	75594473	ID=Msa0225470;Name=Msa0225470
chr2_1	mRNA	75594473	75595487	75594473	ID=Msa0225470-mRNA-1;Parent=Msa0225470;Name=Msa0225470-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|1\|1\|1\|5\|0\|164
chr2_1	exon	75594473	75594541	75594473	ID=Msa0225470-mRNA-1:exon:22656;Parent=Msa0225470-mRNA-1
chr2_1	exon	75594708	75594839	75594708	ID=Msa0225470-mRNA-1:exon:22657;Parent=Msa0225470-mRNA-1
chr2_1	exon	75594925	75595073	75594925	ID=Msa0225470-mRNA-1:exon:22658;Parent=Msa0225470-mRNA-1
chr2_1	exon	75595246	75595314	75595246	ID=Msa0225470-mRNA-1:exon:22659;Parent=Msa0225470-mRNA-1
chr2_1	exon	75595412	75595487	75595412	ID=Msa0225470-mRNA-1:exon:22660;Parent=Msa0225470-mRNA-1
chr2_1	CDS	75594473	75594541	75594473	ID=Msa0225470-mRNA-1:cds;Parent=Msa0225470-mRNA-1
chr2_1	CDS	75594708	75594839	75594708	ID=Msa0225470-mRNA-1:cds;Parent=Msa0225470-mRNA-1
chr2_1	CDS	75594925	75595073	75594925	ID=Msa0225470-mRNA-1:cds;Parent=Msa0225470-mRNA-1
chr2_1	CDS	75595246	75595314	75595246	ID=Msa0225470-mRNA-1:cds;Parent=Msa0225470-mRNA-1
chr2_1	CDS	75595412	75595487	75595412	ID=Msa0225470-mRNA-1:cds;Parent=Msa0225470-mRNA-1

Gene Sequence

>Msa0225470

ATGCAAGTGGCATTTAATCCCAAGGATCCATCTACCTTTGCCAGTGCATCCCTTGATGGCACTTTAAAGATTTGGTCTATTGATTCCTCAACTCCAAATTTTACCTTTGAAGGACACTTGAAAGGAGTGAATTGTGTAGACTATTTTGAAAGCAATGATAAGCAATATCTTTTAAGTGGTTCTGATGATTACACTGCCAAGGTATGGGATTATGATTCTAAAAACTGTGTACAAACACTTGAAGGACACAAAAATAATGTTACAGCAATATGTGCTCATCCTGAGATACCTATAATAATAACTGCTTCAGAGGATTCGACTGTTAAGATTTGGGATGCTGTCACCTATAGGCTTCAAAACACATTGGACTTTGGTCTTGAGAGAGTATGGAGTATTGGCTACAAAAAAGGATCATCTGAACTTGCTTTTGGTTGTGACAAAGGATTTATCATGGTTACAGTGAACTCTGCAACTTGCCAAGTGAAACAAGATTAA

Protein sequence

>Msa0225470

MQVAFNPKDPSTFASASLDGTLKIWSIDSSTPNFTFEGHLKGVNCVDYFESNDKQYLLSGSDDYTAKVWDYDSKNCVQTLEGHKNNVTAICAHPEIPIIITASEDSTVKIWDAVTYRLQNTLDFGLERVWSIGYKKGSSELAFGCDKGFIMVTVNSATCQVKQD*