AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1251240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251240 MtrunA17_Chr8g0366361 95.714 210 9 0 1 210 1 210 1.48e-148 411
Msa1251240 MtrunA17_Chr5g0410801 54.589 207 78 2 1 207 1 191 9.48e-73 218
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251240 AT1G32260.1 49.761 209 92 4 1 206 1 199 1.99e-58 183
Msa1251240 AT2G35480.1 42.584 209 101 6 1 207 1 192 5.89e-43 143
Msa1251240 AT2G35480.2 36.310 168 88 6 42 207 11 161 7.21e-19 80.5

Find 74 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTCAGGCGGTGTTATT+AGG 0.268752 8_2:+47029396 Msa1251240:CDS
CAAGTCACTGTTTCTCTTTG+TGG 0.288311 8_2:-47029470 None:intergenic
TTACGCCATCATCAAGTATA+TGG 0.301117 8_2:+47029252 Msa1251240:CDS
CAAGTTTCTTTACATTTCTA+AGG 0.311759 8_2:-47027568 None:intergenic
TCTCTTTGTGGATAATAAAC+TGG 0.325703 8_2:-47029458 None:intergenic
GACATAAGTATTGCAGTTGC+TGG 0.329674 8_2:+47029494 Msa1251240:three_prime_UTR
AAATTAATATGTAGAGCTTC+TGG 0.353355 8_2:-47029534 None:intergenic
AAACCGTCAGCTCAGATTCT+TGG 0.389574 8_2:+47029068 Msa1251240:CDS
GCAATGGTGGGGAAAGGCTT+TGG 0.392394 8_2:-47029275 None:intergenic
GTGGTCATCCCAGCAGGATT+AGG 0.393910 8_2:-47029314 None:intergenic
TTTGCAGCATCAACCATTGC+AGG 0.408698 8_2:-47029427 None:intergenic
CTGGGATGACCACTCATTAT+CGG 0.412313 8_2:+47029324 Msa1251240:CDS
AACCACAATGCTCGTTGGTC+AGG 0.423095 8_2:+47029383 Msa1251240:CDS
TGGTTAAAAGAACTAGCTAT+TGG 0.438906 8_2:+47029677 Msa1251240:three_prime_UTR
GACGGGTCTGAGGATGATTA+CGG 0.440079 8_2:-47027791 None:intergenic
TTCATCAGAAAATTCAAGAA+AGG 0.454213 8_2:+47028988 Msa1251240:CDS
CGCAAAATACTTGCAATGGT+GGG 0.455115 8_2:-47029287 None:intergenic
GAACTTACAGACACTCTCTA+AGG 0.460937 8_2:+47027748 Msa1251240:CDS
TTGCGACCACCTAATCCTGC+TGG 0.473916 8_2:+47029305 Msa1251240:CDS
CACCGCGATCATGGTGTTGT+GGG 0.474034 8_2:+47027686 Msa1251240:exon
CAAAGAAACTAAACGAAAAG+AGG 0.481706 8_2:-47029589 None:intergenic
ACAGAAGTGGGTCGGAATCT+CGG 0.496651 8_2:+47029014 Msa1251240:CDS
ACGGGTCTGAGGATGATTAC+GGG 0.501177 8_2:-47027790 None:intergenic
TCATCAGAAAATTCAAGAAA+GGG 0.505637 8_2:+47028989 Msa1251240:CDS
TATAAGAAACACCGCGATCA+TGG 0.507384 8_2:+47027677 Msa1251240:exon
TAATGAGTGGTCATCCCAGC+AGG 0.515172 8_2:-47029320 None:intergenic
TCGGAAACTTATTGCTGCGA+TGG 0.516527 8_2:+47029033 Msa1251240:CDS
TTTGGCCATATACTTGATGA+TGG 0.522132 8_2:-47029257 None:intergenic
TGGTTGATGCTGCAAAACTA+AGG 0.523042 8_2:+47029434 Msa1251240:CDS
CCGTCAGCTCAGATTCTTGG+TGG 0.524373 8_2:+47029071 Msa1251240:CDS
TTACTATGCTTTATTCAAGA+GGG 0.527984 8_2:+47029211 Msa1251240:CDS
ATTATTATTATTCAACAGGA+TGG 0.528872 8_2:-47029714 None:intergenic
CTTGGTGGATCACCCACCAC+TGG 0.535629 8_2:+47029086 Msa1251240:CDS
TTGAATAAAGCATAGTAACT+AGG 0.542478 8_2:-47029164 None:intergenic
TTGAATAAAGCATAGTAACT+AGG 0.542478 8_2:-47029206 None:intergenic
ACACCGCGATCATGGTGTTG+TGG 0.543146 8_2:+47027685 Msa1251240:exon
GTTACTATGCTTTATTCAAG+AGG 0.545448 8_2:+47029210 Msa1251240:CDS
TAGTCTGTTGAGCTTCCTTG+TGG 0.546609 8_2:+47029621 Msa1251240:three_prime_UTR
TGCGACCACCTAATCCTGCT+GGG 0.548349 8_2:+47029306 Msa1251240:CDS
AGACCCGTCAATTTCTTCAC+CGG 0.552293 8_2:+47027805 Msa1251240:CDS
ATACTTGCAATGGTGGGGAA+AGG 0.558932 8_2:-47029281 None:intergenic
GAAACTTGTGCAACAATGCA+AGG 0.560329 8_2:+47027583 Msa1251240:five_prime_UTR
GTCACTGCGATCGCGAATTG+TGG 0.560738 8_2:+47027542 Msa1251240:five_prime_UTR
TCGCAAAATACTTGCAATGG+TGG 0.568555 8_2:-47029288 None:intergenic
CACAATGCTCGTTGGTCAGG+CGG 0.569223 8_2:+47029386 Msa1251240:CDS
TACTATGCTTTATTCAAGAG+GGG 0.576632 8_2:+47029212 Msa1251240:CDS
GCAAAATACTTGCAATGGTG+GGG 0.578377 8_2:-47029286 None:intergenic
TCCTTGTGGAGAATGAATTG+TGG 0.579985 8_2:+47029635 Msa1251240:three_prime_UTR
GCTTGAGCTAGCACCAGTGG+TGG 0.581830 8_2:-47029099 None:intergenic
TCAAGAAAGGGACACAGAAG+TGG 0.582812 8_2:+47029001 Msa1251240:CDS
CCACCAAGAATCTGAGCTGA+CGG 0.586371 8_2:-47029071 None:intergenic
TGGTCGCAAAATACTTGCAA+TGG 0.588719 8_2:-47029291 None:intergenic
TACGCTTGAGCTAGCACCAG+TGG 0.596946 8_2:-47029102 None:intergenic
AACGCGTTCTGTATTTGATG+TGG 0.598254 8_2:+47028956 Msa1251240:CDS
ATACCGGTGAAGAAATTGAC+GGG 0.601735 8_2:-47027808 None:intergenic
TGAAGAAATTGACGGGTCTG+AGG 0.603278 8_2:-47027801 None:intergenic
ACTGCGATCGCGAATTGTGG+CGG 0.609328 8_2:+47027545 Msa1251240:five_prime_UTR
GATCAAACCACAATGCTCGT+TGG 0.610476 8_2:+47029378 Msa1251240:CDS
GTCATCCCAGCAGGATTAGG+TGG 0.610999 8_2:-47029311 None:intergenic
GTAATGACAAAGCTTCAAGA+TGG 0.625803 8_2:+47029122 Msa1251240:CDS
GTGTTGTGGGAGATTGCACT+TGG 0.626558 8_2:+47027699 Msa1251240:CDS
AATACCGGTGAAGAAATTGA+CGG 0.628953 8_2:-47027809 None:intergenic
AAAGGGACACAGAAGTGGGT+CGG 0.636704 8_2:+47029006 Msa1251240:CDS
TTAGGAAAGACATCCTGCAA+TGG 0.637537 8_2:+47029414 Msa1251240:CDS
TCCACAATTCATTCTCCACA+AGG 0.640075 8_2:-47029636 None:intergenic
TCAAGAGGGGATCGAATAAG+CGG 0.644249 8_2:+47029225 Msa1251240:CDS
CAAGAAAGGGACACAGAAGT+GGG 0.644643 8_2:+47029002 Msa1251240:CDS
TTTGATCGAAAAGCATCAAG+AGG 0.652615 8_2:-47029362 None:intergenic
CTCCCACAACACCATGATCG+CGG 0.656249 8_2:-47027688 None:intergenic
CATCAAGAGGATAGTAACTG+AGG 0.661219 8_2:-47029349 None:intergenic
CGCCTGACCAACGAGCATTG+TGG 0.670110 8_2:-47029385 None:intergenic
ACTGAGGTTCCGATAATGAG+TGG 0.671740 8_2:-47029333 None:intergenic
CTTGAGCTAGCACCAGTGGT+GGG 0.680988 8_2:-47029098 None:intergenic
ACTCTCTAAGGATTCAACGT+AGG 0.713218 8_2:+47027760 Msa1251240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATAAAATTGAAAAG+GGG - chr8_2:47027842-47027861 None:intergenic 10.0%
!! CAAAATTAATAAAATTGAAA+AGG - chr8_2:47027844-47027863 None:intergenic 10.0%
!! AAATTAATAAAATTGAAAAG+GGG - chr8_2:47027842-47027861 None:intergenic 10.0%
!! CAAAATTAATAAAATTGAAA+AGG - chr8_2:47027844-47027863 None:intergenic 10.0%
!! ACAAAACATTTATTATATTG+CGG + chr8_2:47028178-47028197 Msa1251240:intron 15.0%
!! ACAAAACATTTATTATATTG+CGG + chr8_2:47028178-47028197 Msa1251240:intron 15.0%
!!! AAAAGTGCAATTTTAAATTG+CGG - chr8_2:47028252-47028271 None:intergenic 20.0%
!! ATTGAGATTCTATTTGTTTA+AGG + chr8_2:47028438-47028457 Msa1251240:intron 20.0%
!!! ATTCTATTTGTTTAAGGTTT+AGG + chr8_2:47028444-47028463 Msa1251240:intron 20.0%
!!! TTCTATTTGTTTAAGGTTTA+GGG + chr8_2:47028445-47028464 Msa1251240:intron 20.0%
!! AAAAAGAATCTCATTAACTT+TGG + chr8_2:47028539-47028558 Msa1251240:intron 20.0%
!! AAAAGCAAATTAGAGAAAAA+GGG - chr8_2:47028834-47028853 None:intergenic 20.0%
!!! AAAAGTGCAATTTTAAATTG+CGG - chr8_2:47028252-47028271 None:intergenic 20.0%
!! ATTGAGATTCTATTTGTTTA+AGG + chr8_2:47028438-47028457 Msa1251240:intron 20.0%
!!! ATTCTATTTGTTTAAGGTTT+AGG + chr8_2:47028444-47028463 Msa1251240:intron 20.0%
!!! TTCTATTTGTTTAAGGTTTA+GGG + chr8_2:47028445-47028464 Msa1251240:intron 20.0%
!! AAAAAGAATCTCATTAACTT+TGG + chr8_2:47028539-47028558 Msa1251240:intron 20.0%
!! AAAAGCAAATTAGAGAAAAA+GGG - chr8_2:47028834-47028853 None:intergenic 20.0%
! CAAGTTTCTTTACATTTCTA+AGG - chr8_2:47027571-47027590 None:intergenic 25.0%
! ATAATTTACTTTCGCTCAAT+TGG - chr8_2:47027900-47027919 None:intergenic 25.0%
!!! TTCTTTTGAAAGTGATAACT+TGG + chr8_2:47028105-47028124 Msa1251240:intron 25.0%
! CGGTAAAATATGATAAAATG+TGG + chr8_2:47028198-47028217 Msa1251240:intron 25.0%
!!! ACAGCGATAAATTTGTTATT+AGG + chr8_2:47028364-47028383 Msa1251240:intron 25.0%
!!! TTTTTTCATTAAGTCGTTCA+CGG - chr8_2:47028517-47028536 None:intergenic 25.0%
!!! ATTTTAGAGCAAATAACATC+AGG - chr8_2:47028578-47028597 None:intergenic 25.0%
! AAAGCAAATTAGAGAAAAAG+GGG - chr8_2:47028833-47028852 None:intergenic 25.0%
! CAAAAGCAAATTAGAGAAAA+AGG - chr8_2:47028835-47028854 None:intergenic 25.0%
!!! CTTTTTCTCTAATTTGCTTT+TGG + chr8_2:47028833-47028852 Msa1251240:intron 25.0%
!!! ATTGATTTGTTTGCGTTATT+AGG + chr8_2:47028874-47028893 Msa1251240:intron 25.0%
! TTCATCAGAAAATTCAAGAA+AGG + chr8_2:47028988-47029007 Msa1251240:CDS 25.0%
! TCATCAGAAAATTCAAGAAA+GGG + chr8_2:47028989-47029008 Msa1251240:CDS 25.0%
! TTGAATAAAGCATAGTAACT+AGG - chr8_2:47029167-47029186 None:intergenic 25.0%
! TTGAATAAAGCATAGTAACT+AGG - chr8_2:47029167-47029186 None:intergenic 25.0%
!! TTACTATGCTTTATTCAAGA+GGG + chr8_2:47029169-47029188 Msa1251240:CDS 25.0%
! AAATTAATATGTAGAGCTTC+TGG - chr8_2:47029537-47029556 None:intergenic 25.0%
!! GAACATTTGTTTGTAGTTTT+TGG + chr8_2:47029657-47029676 Msa1251240:three_prime_UTR 25.0%
! CAAGTTTCTTTACATTTCTA+AGG - chr8_2:47027571-47027590 None:intergenic 25.0%
! ATAATTTACTTTCGCTCAAT+TGG - chr8_2:47027900-47027919 None:intergenic 25.0%
!!! TTCTTTTGAAAGTGATAACT+TGG + chr8_2:47028105-47028124 Msa1251240:intron 25.0%
! CGGTAAAATATGATAAAATG+TGG + chr8_2:47028198-47028217 Msa1251240:intron 25.0%
!!! ACAGCGATAAATTTGTTATT+AGG + chr8_2:47028364-47028383 Msa1251240:intron 25.0%
!!! TTTTTTCATTAAGTCGTTCA+CGG - chr8_2:47028517-47028536 None:intergenic 25.0%
!!! ATTTTAGAGCAAATAACATC+AGG - chr8_2:47028578-47028597 None:intergenic 25.0%
! AAAGCAAATTAGAGAAAAAG+GGG - chr8_2:47028833-47028852 None:intergenic 25.0%
! CAAAAGCAAATTAGAGAAAA+AGG - chr8_2:47028835-47028854 None:intergenic 25.0%
!!! CTTTTTCTCTAATTTGCTTT+TGG + chr8_2:47028833-47028852 Msa1251240:intron 25.0%
!!! ATTGATTTGTTTGCGTTATT+AGG + chr8_2:47028874-47028893 Msa1251240:intron 25.0%
! TTCATCAGAAAATTCAAGAA+AGG + chr8_2:47028988-47029007 Msa1251240:CDS 25.0%
! TCATCAGAAAATTCAAGAAA+GGG + chr8_2:47028989-47029008 Msa1251240:CDS 25.0%
! TTGAATAAAGCATAGTAACT+AGG - chr8_2:47029167-47029186 None:intergenic 25.0%
! TTGAATAAAGCATAGTAACT+AGG - chr8_2:47029167-47029186 None:intergenic 25.0%
!! TTACTATGCTTTATTCAAGA+GGG + chr8_2:47029169-47029188 Msa1251240:CDS 25.0%
! AAATTAATATGTAGAGCTTC+TGG - chr8_2:47029537-47029556 None:intergenic 25.0%
!! GAACATTTGTTTGTAGTTTT+TGG + chr8_2:47029657-47029676 Msa1251240:three_prime_UTR 25.0%
!!! ACTTGGAACTGCATATTTTT+TGG + chr8_2:47027716-47027735 Msa1251240:CDS 30.0%
!!! CTTGGAACTGCATATTTTTT+GGG + chr8_2:47027717-47027736 Msa1251240:CDS 30.0%
! ACATGGTAACATTTTTCTCA+CGG - chr8_2:47027966-47027985 None:intergenic 30.0%
ATGACAAAAACAAACTCACA+TGG - chr8_2:47027983-47028002 None:intergenic 30.0%
TTTGTAGAAAGCTTAAAACG+TGG + chr8_2:47028279-47028298 Msa1251240:intron 30.0%
!! GCGATAAATTTGTTATTAGG+AGG + chr8_2:47028367-47028386 Msa1251240:intron 30.0%
! TTTTTCATTAAGTCGTTCAC+GGG - chr8_2:47028516-47028535 None:intergenic 30.0%
AAATTAGAGAAAAAGGGGAA+AGG - chr8_2:47028828-47028847 None:intergenic 30.0%
! CATTATCTGTCTTCATGTTT+TGG + chr8_2:47028920-47028939 Msa1251240:intron 30.0%
! GTTACTATGCTTTATTCAAG+AGG + chr8_2:47029168-47029187 Msa1251240:CDS 30.0%
! TACTATGCTTTATTCAAGAG+GGG + chr8_2:47029170-47029189 Msa1251240:CDS 30.0%
TCTCTTTGTGGATAATAAAC+TGG - chr8_2:47029461-47029480 None:intergenic 30.0%
CAAAGAAACTAAACGAAAAG+AGG - chr8_2:47029592-47029611 None:intergenic 30.0%
!! CTCTTTTCGTTTAGTTTCTT+TGG + chr8_2:47029590-47029609 Msa1251240:three_prime_UTR 30.0%
TGGTTAAAAGAACTAGCTAT+TGG + chr8_2:47029677-47029696 Msa1251240:three_prime_UTR 30.0%
!!! ACTTGGAACTGCATATTTTT+TGG + chr8_2:47027716-47027735 Msa1251240:CDS 30.0%
!!! CTTGGAACTGCATATTTTTT+GGG + chr8_2:47027717-47027736 Msa1251240:CDS 30.0%
! ACATGGTAACATTTTTCTCA+CGG - chr8_2:47027966-47027985 None:intergenic 30.0%
ATGACAAAAACAAACTCACA+TGG - chr8_2:47027983-47028002 None:intergenic 30.0%
TTTGTAGAAAGCTTAAAACG+TGG + chr8_2:47028279-47028298 Msa1251240:intron 30.0%
!! GCGATAAATTTGTTATTAGG+AGG + chr8_2:47028367-47028386 Msa1251240:intron 30.0%
! TTTTTCATTAAGTCGTTCAC+GGG - chr8_2:47028516-47028535 None:intergenic 30.0%
AAATTAGAGAAAAAGGGGAA+AGG - chr8_2:47028828-47028847 None:intergenic 30.0%
! CATTATCTGTCTTCATGTTT+TGG + chr8_2:47028920-47028939 Msa1251240:intron 30.0%
! GTTACTATGCTTTATTCAAG+AGG + chr8_2:47029168-47029187 Msa1251240:CDS 30.0%
! TACTATGCTTTATTCAAGAG+GGG + chr8_2:47029170-47029189 Msa1251240:CDS 30.0%
TCTCTTTGTGGATAATAAAC+TGG - chr8_2:47029461-47029480 None:intergenic 30.0%
CAAAGAAACTAAACGAAAAG+AGG - chr8_2:47029592-47029611 None:intergenic 30.0%
!! CTCTTTTCGTTTAGTTTCTT+TGG + chr8_2:47029590-47029609 Msa1251240:three_prime_UTR 30.0%
TGGTTAAAAGAACTAGCTAT+TGG + chr8_2:47029677-47029696 Msa1251240:three_prime_UTR 30.0%
AATACCGGTGAAGAAATTGA+CGG - chr8_2:47027812-47027831 None:intergenic 35.0%
AAAAGGGGAAGAGTAAATAC+CGG - chr8_2:47027827-47027846 None:intergenic 35.0%
! CATGGTAACATTTTTCTCAC+GGG - chr8_2:47027965-47027984 None:intergenic 35.0%
GAAGCTATAAGCTCAAAATC+TGG + chr8_2:47028012-47028031 Msa1251240:intron 35.0%
ATAAGCTCAAAATCTGGCAT+TGG + chr8_2:47028018-47028037 Msa1251240:intron 35.0%
TAAGCTCAAAATCTGGCATT+GGG + chr8_2:47028019-47028038 Msa1251240:intron 35.0%
CTTTGTCCTAGTAGTTTATG+AGG + chr8_2:47028066-47028085 Msa1251240:intron 35.0%
CCATTTCCTCATAAACTACT+AGG - chr8_2:47028075-47028094 None:intergenic 35.0%
CCTAGTAGTTTATGAGGAAA+TGG + chr8_2:47028072-47028091 Msa1251240:intron 35.0%
TTAAATTGTAGCCGTGATTG+CGG + chr8_2:47028148-47028167 Msa1251240:intron 35.0%
TGTAGTTGCAATTGTGATTG+TGG + chr8_2:47028225-47028244 Msa1251240:intron 35.0%
! TGAATTTGATAACCGTCTCA+TGG + chr8_2:47028328-47028347 Msa1251240:intron 35.0%
ATTAGGAGGTCAAATGTGAA+TGG + chr8_2:47028381-47028400 Msa1251240:intron 35.0%
TCGTAATATCAAGAGAAAGC+TGG + chr8_2:47028602-47028621 Msa1251240:intron 35.0%
! AACATTGTCATTTGCCACAA+TGG - chr8_2:47028673-47028692 None:intergenic 35.0%
TATTTCATCTCTTGTCTGAC+TGG - chr8_2:47028734-47028753 None:intergenic 35.0%
GTAATGACAAAGCTTCAAGA+TGG + chr8_2:47029122-47029141 Msa1251240:CDS 35.0%
! CATAGTAACTAGGTCTTTTG+AGG - chr8_2:47029157-47029176 None:intergenic 35.0%
! CATAGTAACTAGGTCTTTTG+AGG - chr8_2:47029157-47029176 None:intergenic 35.0%
TTACGCCATCATCAAGTATA+TGG + chr8_2:47029252-47029271 Msa1251240:CDS 35.0%
TTTGGCCATATACTTGATGA+TGG - chr8_2:47029260-47029279 None:intergenic 35.0%
!! TTTGATCGAAAAGCATCAAG+AGG - chr8_2:47029365-47029384 None:intergenic 35.0%
GTTGCTGGAAAAACAATTGT+TGG + chr8_2:47029509-47029528 Msa1251240:three_prime_UTR 35.0%
AATACCGGTGAAGAAATTGA+CGG - chr8_2:47027812-47027831 None:intergenic 35.0%
AAAAGGGGAAGAGTAAATAC+CGG - chr8_2:47027827-47027846 None:intergenic 35.0%
! CATGGTAACATTTTTCTCAC+GGG - chr8_2:47027965-47027984 None:intergenic 35.0%
GAAGCTATAAGCTCAAAATC+TGG + chr8_2:47028012-47028031 Msa1251240:intron 35.0%
ATAAGCTCAAAATCTGGCAT+TGG + chr8_2:47028018-47028037 Msa1251240:intron 35.0%
TAAGCTCAAAATCTGGCATT+GGG + chr8_2:47028019-47028038 Msa1251240:intron 35.0%
CTTTGTCCTAGTAGTTTATG+AGG + chr8_2:47028066-47028085 Msa1251240:intron 35.0%
CCATTTCCTCATAAACTACT+AGG - chr8_2:47028075-47028094 None:intergenic 35.0%
CCTAGTAGTTTATGAGGAAA+TGG + chr8_2:47028072-47028091 Msa1251240:intron 35.0%
TTAAATTGTAGCCGTGATTG+CGG + chr8_2:47028148-47028167 Msa1251240:intron 35.0%
TGTAGTTGCAATTGTGATTG+TGG + chr8_2:47028225-47028244 Msa1251240:intron 35.0%
! TGAATTTGATAACCGTCTCA+TGG + chr8_2:47028328-47028347 Msa1251240:intron 35.0%
ATTAGGAGGTCAAATGTGAA+TGG + chr8_2:47028381-47028400 Msa1251240:intron 35.0%
TCGTAATATCAAGAGAAAGC+TGG + chr8_2:47028602-47028621 Msa1251240:intron 35.0%
! AACATTGTCATTTGCCACAA+TGG - chr8_2:47028673-47028692 None:intergenic 35.0%
TATTTCATCTCTTGTCTGAC+TGG - chr8_2:47028734-47028753 None:intergenic 35.0%
GTAATGACAAAGCTTCAAGA+TGG + chr8_2:47029122-47029141 Msa1251240:CDS 35.0%
! CATAGTAACTAGGTCTTTTG+AGG - chr8_2:47029157-47029176 None:intergenic 35.0%
! CATAGTAACTAGGTCTTTTG+AGG - chr8_2:47029157-47029176 None:intergenic 35.0%
TTACGCCATCATCAAGTATA+TGG + chr8_2:47029252-47029271 Msa1251240:CDS 35.0%
TTTGGCCATATACTTGATGA+TGG - chr8_2:47029260-47029279 None:intergenic 35.0%
!! TTTGATCGAAAAGCATCAAG+AGG - chr8_2:47029365-47029384 None:intergenic 35.0%
GTTGCTGGAAAAACAATTGT+TGG + chr8_2:47029509-47029528 Msa1251240:three_prime_UTR 35.0%
GAAACTTGTGCAACAATGCA+AGG + chr8_2:47027583-47027602 Msa1251240:five_prime_UTR 40.0%
TATAAGAAACACCGCGATCA+TGG + chr8_2:47027677-47027696 Msa1251240:exon 40.0%
GAACTTACAGACACTCTCTA+AGG + chr8_2:47027748-47027767 Msa1251240:CDS 40.0%
! ACTCTCTAAGGATTCAACGT+AGG + chr8_2:47027760-47027779 Msa1251240:CDS 40.0%
ATACCGGTGAAGAAATTGAC+GGG - chr8_2:47027811-47027830 None:intergenic 40.0%
TTGTAGTGTAACCGCAATCA+CGG - chr8_2:47028162-47028181 None:intergenic 40.0%
ATGTGAATGGCAACGTATAG+TGG + chr8_2:47028394-47028413 Msa1251240:intron 40.0%
CTATAGCAGGGTCTATGTAA+GGG + chr8_2:47028641-47028660 Msa1251240:intron 40.0%
AACGCGTTCTGTATTTGATG+TGG + chr8_2:47028956-47028975 Msa1251240:CDS 40.0%
!!! GTAACTAGGTCTTTTGAGGT+TGG - chr8_2:47029153-47029172 None:intergenic 40.0%
!!! GTAACTAGGTCTTTTGAGGT+TGG - chr8_2:47029153-47029172 None:intergenic 40.0%
GCAAAATACTTGCAATGGTG+GGG - chr8_2:47029289-47029308 None:intergenic 40.0%
CGCAAAATACTTGCAATGGT+GGG - chr8_2:47029290-47029309 None:intergenic 40.0%
TCGCAAAATACTTGCAATGG+TGG - chr8_2:47029291-47029310 None:intergenic 40.0%
TGGTCGCAAAATACTTGCAA+TGG - chr8_2:47029294-47029313 None:intergenic 40.0%
CATCAAGAGGATAGTAACTG+AGG - chr8_2:47029352-47029371 None:intergenic 40.0%
TTAGGAAAGACATCCTGCAA+TGG + chr8_2:47029414-47029433 Msa1251240:CDS 40.0%
! TGGTTGATGCTGCAAAACTA+AGG + chr8_2:47029434-47029453 Msa1251240:CDS 40.0%
! CAAGTCACTGTTTCTCTTTG+TGG - chr8_2:47029473-47029492 None:intergenic 40.0%
! GACATAAGTATTGCAGTTGC+TGG + chr8_2:47029494-47029513 Msa1251240:three_prime_UTR 40.0%
TCCTTGTGGAGAATGAATTG+TGG + chr8_2:47029635-47029654 Msa1251240:three_prime_UTR 40.0%
TCCACAATTCATTCTCCACA+AGG - chr8_2:47029639-47029658 None:intergenic 40.0%
GAAACTTGTGCAACAATGCA+AGG + chr8_2:47027583-47027602 Msa1251240:five_prime_UTR 40.0%
TATAAGAAACACCGCGATCA+TGG + chr8_2:47027677-47027696 Msa1251240:exon 40.0%
GAACTTACAGACACTCTCTA+AGG + chr8_2:47027748-47027767 Msa1251240:CDS 40.0%
! ACTCTCTAAGGATTCAACGT+AGG + chr8_2:47027760-47027779 Msa1251240:CDS 40.0%
ATACCGGTGAAGAAATTGAC+GGG - chr8_2:47027811-47027830 None:intergenic 40.0%
TTGTAGTGTAACCGCAATCA+CGG - chr8_2:47028162-47028181 None:intergenic 40.0%
ATGTGAATGGCAACGTATAG+TGG + chr8_2:47028394-47028413 Msa1251240:intron 40.0%
CTATAGCAGGGTCTATGTAA+GGG + chr8_2:47028641-47028660 Msa1251240:intron 40.0%
AACGCGTTCTGTATTTGATG+TGG + chr8_2:47028956-47028975 Msa1251240:CDS 40.0%
!!! GTAACTAGGTCTTTTGAGGT+TGG - chr8_2:47029153-47029172 None:intergenic 40.0%
!!! GTAACTAGGTCTTTTGAGGT+TGG - chr8_2:47029153-47029172 None:intergenic 40.0%
GCAAAATACTTGCAATGGTG+GGG - chr8_2:47029289-47029308 None:intergenic 40.0%
CGCAAAATACTTGCAATGGT+GGG - chr8_2:47029290-47029309 None:intergenic 40.0%
TCGCAAAATACTTGCAATGG+TGG - chr8_2:47029291-47029310 None:intergenic 40.0%
TGGTCGCAAAATACTTGCAA+TGG - chr8_2:47029294-47029313 None:intergenic 40.0%
CATCAAGAGGATAGTAACTG+AGG - chr8_2:47029352-47029371 None:intergenic 40.0%
TTAGGAAAGACATCCTGCAA+TGG + chr8_2:47029414-47029433 Msa1251240:CDS 40.0%
! TGGTTGATGCTGCAAAACTA+AGG + chr8_2:47029434-47029453 Msa1251240:CDS 40.0%
! CAAGTCACTGTTTCTCTTTG+TGG - chr8_2:47029473-47029492 None:intergenic 40.0%
! GACATAAGTATTGCAGTTGC+TGG + chr8_2:47029494-47029513 Msa1251240:three_prime_UTR 40.0%
TCCTTGTGGAGAATGAATTG+TGG + chr8_2:47029635-47029654 Msa1251240:three_prime_UTR 40.0%
TCCACAATTCATTCTCCACA+AGG - chr8_2:47029639-47029658 None:intergenic 40.0%
! TGAAGAAATTGACGGGTCTG+AGG - chr8_2:47027804-47027823 None:intergenic 45.0%
AGACCCGTCAATTTCTTCAC+CGG + chr8_2:47027805-47027824 Msa1251240:CDS 45.0%
!! TGATAACTTGGTGCAGCTGT+TGG + chr8_2:47028117-47028136 Msa1251240:intron 45.0%
CGTGGTGTCTTCAATTGATG+TGG + chr8_2:47028297-47028316 Msa1251240:intron 45.0%
ACCGTCTCATGGAATTGTGA+TGG + chr8_2:47028339-47028358 Msa1251240:intron 45.0%
CCCATCACAATTCCATGAGA+CGG - chr8_2:47028343-47028362 None:intergenic 45.0%
CCGTCTCATGGAATTGTGAT+GGG + chr8_2:47028340-47028359 Msa1251240:intron 45.0%
GCTATAGCAGGGTCTATGTA+AGG + chr8_2:47028640-47028659 Msa1251240:intron 45.0%
TGTAAGGGTTGGATCCATTG+TGG + chr8_2:47028656-47028675 Msa1251240:intron 45.0%
TCAAGAAAGGGACACAGAAG+TGG + chr8_2:47029001-47029020 Msa1251240:CDS 45.0%
CAAGAAAGGGACACAGAAGT+GGG + chr8_2:47029002-47029021 Msa1251240:CDS 45.0%
TCGGAAACTTATTGCTGCGA+TGG + chr8_2:47029033-47029052 Msa1251240:CDS 45.0%
AAACCGTCAGCTCAGATTCT+TGG + chr8_2:47029068-47029087 Msa1251240:CDS 45.0%
!!! ACTAGGTCTTTTGAGGTTGG+AGG - chr8_2:47029150-47029169 None:intergenic 45.0%
TCAAGAGGGGATCGAATAAG+CGG + chr8_2:47029225-47029244 Msa1251240:CDS 45.0%
! ATACTTGCAATGGTGGGGAA+AGG - chr8_2:47029284-47029303 None:intergenic 45.0%
CTGGGATGACCACTCATTAT+CGG + chr8_2:47029324-47029343 Msa1251240:CDS 45.0%
ACTGAGGTTCCGATAATGAG+TGG - chr8_2:47029336-47029355 None:intergenic 45.0%
GATCAAACCACAATGCTCGT+TGG + chr8_2:47029378-47029397 Msa1251240:CDS 45.0%
TTTGCAGCATCAACCATTGC+AGG - chr8_2:47029430-47029449 None:intergenic 45.0%
TAGTCTGTTGAGCTTCCTTG+TGG + chr8_2:47029621-47029640 Msa1251240:three_prime_UTR 45.0%
! TGAAGAAATTGACGGGTCTG+AGG - chr8_2:47027804-47027823 None:intergenic 45.0%
AGACCCGTCAATTTCTTCAC+CGG + chr8_2:47027805-47027824 Msa1251240:CDS 45.0%
!! TGATAACTTGGTGCAGCTGT+TGG + chr8_2:47028117-47028136 Msa1251240:intron 45.0%
CGTGGTGTCTTCAATTGATG+TGG + chr8_2:47028297-47028316 Msa1251240:intron 45.0%
ACCGTCTCATGGAATTGTGA+TGG + chr8_2:47028339-47028358 Msa1251240:intron 45.0%
CCCATCACAATTCCATGAGA+CGG - chr8_2:47028343-47028362 None:intergenic 45.0%
CCGTCTCATGGAATTGTGAT+GGG + chr8_2:47028340-47028359 Msa1251240:intron 45.0%
GCTATAGCAGGGTCTATGTA+AGG + chr8_2:47028640-47028659 Msa1251240:intron 45.0%
TGTAAGGGTTGGATCCATTG+TGG + chr8_2:47028656-47028675 Msa1251240:intron 45.0%
TCAAGAAAGGGACACAGAAG+TGG + chr8_2:47029001-47029020 Msa1251240:CDS 45.0%
CAAGAAAGGGACACAGAAGT+GGG + chr8_2:47029002-47029021 Msa1251240:CDS 45.0%
TCGGAAACTTATTGCTGCGA+TGG + chr8_2:47029033-47029052 Msa1251240:CDS 45.0%
AAACCGTCAGCTCAGATTCT+TGG + chr8_2:47029068-47029087 Msa1251240:CDS 45.0%
!!! ACTAGGTCTTTTGAGGTTGG+AGG - chr8_2:47029150-47029169 None:intergenic 45.0%
TCAAGAGGGGATCGAATAAG+CGG + chr8_2:47029225-47029244 Msa1251240:CDS 45.0%
! ATACTTGCAATGGTGGGGAA+AGG - chr8_2:47029284-47029303 None:intergenic 45.0%
CTGGGATGACCACTCATTAT+CGG + chr8_2:47029324-47029343 Msa1251240:CDS 45.0%
ACTGAGGTTCCGATAATGAG+TGG - chr8_2:47029336-47029355 None:intergenic 45.0%
GATCAAACCACAATGCTCGT+TGG + chr8_2:47029378-47029397 Msa1251240:CDS 45.0%
TTTGCAGCATCAACCATTGC+AGG - chr8_2:47029430-47029449 None:intergenic 45.0%
TAGTCTGTTGAGCTTCCTTG+TGG + chr8_2:47029621-47029640 Msa1251240:three_prime_UTR 45.0%
!! AAAATTAATAAAATTGAAAA+GGG - chr8_2:47027843-47027862 None:intergenic 5.0%
!! AAAATTAATAAAATTGAAAA+GGG - chr8_2:47027843-47027862 None:intergenic 5.0%
GTGTTGTGGGAGATTGCACT+TGG + chr8_2:47027699-47027718 Msa1251240:CDS 50.0%
ACGGGTCTGAGGATGATTAC+GGG - chr8_2:47027793-47027812 None:intergenic 50.0%
GACGGGTCTGAGGATGATTA+CGG - chr8_2:47027794-47027813 None:intergenic 50.0%
! CTTGGTGCAGCTGTTGGTTA+TGG + chr8_2:47028123-47028142 Msa1251240:intron 50.0%
GTTCACGGGTGCGAATAACA+GGG - chr8_2:47028502-47028521 None:intergenic 50.0%
AACGAAGCTTGCGCTATAGC+AGG + chr8_2:47028628-47028647 Msa1251240:intron 50.0%
ACGAAGCTTGCGCTATAGCA+GGG + chr8_2:47028629-47028648 Msa1251240:intron 50.0%
AGCAGGGTCTATGTAAGGGT+TGG + chr8_2:47028645-47028664 Msa1251240:intron 50.0%
AAAGGGACACAGAAGTGGGT+CGG + chr8_2:47029006-47029025 Msa1251240:CDS 50.0%
ACAGAAGTGGGTCGGAATCT+CGG + chr8_2:47029014-47029033 Msa1251240:CDS 50.0%
CCACCAAGAATCTGAGCTGA+CGG - chr8_2:47029074-47029093 None:intergenic 50.0%
TAATGAGTGGTCATCCCAGC+AGG - chr8_2:47029323-47029342 None:intergenic 50.0%
AACCACAATGCTCGTTGGTC+AGG + chr8_2:47029383-47029402 Msa1251240:CDS 50.0%
!! GTTGGTCAGGCGGTGTTATT+AGG + chr8_2:47029396-47029415 Msa1251240:CDS 50.0%
GTGTTGTGGGAGATTGCACT+TGG + chr8_2:47027699-47027718 Msa1251240:CDS 50.0%
ACGGGTCTGAGGATGATTAC+GGG - chr8_2:47027793-47027812 None:intergenic 50.0%
GACGGGTCTGAGGATGATTA+CGG - chr8_2:47027794-47027813 None:intergenic 50.0%
! CTTGGTGCAGCTGTTGGTTA+TGG + chr8_2:47028123-47028142 Msa1251240:intron 50.0%
GTTCACGGGTGCGAATAACA+GGG - chr8_2:47028502-47028521 None:intergenic 50.0%
AACGAAGCTTGCGCTATAGC+AGG + chr8_2:47028628-47028647 Msa1251240:intron 50.0%
ACGAAGCTTGCGCTATAGCA+GGG + chr8_2:47028629-47028648 Msa1251240:intron 50.0%
AGCAGGGTCTATGTAAGGGT+TGG + chr8_2:47028645-47028664 Msa1251240:intron 50.0%
AAAGGGACACAGAAGTGGGT+CGG + chr8_2:47029006-47029025 Msa1251240:CDS 50.0%
ACAGAAGTGGGTCGGAATCT+CGG + chr8_2:47029014-47029033 Msa1251240:CDS 50.0%
CCACCAAGAATCTGAGCTGA+CGG - chr8_2:47029074-47029093 None:intergenic 50.0%
TAATGAGTGGTCATCCCAGC+AGG - chr8_2:47029323-47029342 None:intergenic 50.0%
AACCACAATGCTCGTTGGTC+AGG + chr8_2:47029383-47029402 Msa1251240:CDS 50.0%
!! GTTGGTCAGGCGGTGTTATT+AGG + chr8_2:47029396-47029415 Msa1251240:CDS 50.0%
GTCACTGCGATCGCGAATTG+TGG + chr8_2:47027542-47027561 Msa1251240:five_prime_UTR 55.0%
ACTGCGATCGCGAATTGTGG+CGG + chr8_2:47027545-47027564 Msa1251240:five_prime_UTR 55.0%
! ACACCGCGATCATGGTGTTG+TGG + chr8_2:47027685-47027704 Msa1251240:exon 55.0%
!! CACCGCGATCATGGTGTTGT+GGG + chr8_2:47027686-47027705 Msa1251240:exon 55.0%
CTCCCACAACACCATGATCG+CGG - chr8_2:47027691-47027710 None:intergenic 55.0%
GGGTGCGAATAACAGGGAAG+TGG - chr8_2:47028496-47028515 None:intergenic 55.0%
CGTTCACGGGTGCGAATAAC+AGG - chr8_2:47028503-47028522 None:intergenic 55.0%
CCGTCAGCTCAGATTCTTGG+TGG + chr8_2:47029071-47029090 Msa1251240:CDS 55.0%
CTTGAGCTAGCACCAGTGGT+GGG - chr8_2:47029101-47029120 None:intergenic 55.0%
TACGCTTGAGCTAGCACCAG+TGG - chr8_2:47029105-47029124 None:intergenic 55.0%
! GCAATGGTGGGGAAAGGCTT+TGG - chr8_2:47029278-47029297 None:intergenic 55.0%
TTGCGACCACCTAATCCTGC+TGG + chr8_2:47029305-47029324 Msa1251240:CDS 55.0%
TGCGACCACCTAATCCTGCT+GGG + chr8_2:47029306-47029325 Msa1251240:CDS 55.0%
GTCATCCCAGCAGGATTAGG+TGG - chr8_2:47029314-47029333 None:intergenic 55.0%
GTGGTCATCCCAGCAGGATT+AGG - chr8_2:47029317-47029336 None:intergenic 55.0%
CACAATGCTCGTTGGTCAGG+CGG + chr8_2:47029386-47029405 Msa1251240:CDS 55.0%
GTCACTGCGATCGCGAATTG+TGG + chr8_2:47027542-47027561 Msa1251240:five_prime_UTR 55.0%
ACTGCGATCGCGAATTGTGG+CGG + chr8_2:47027545-47027564 Msa1251240:five_prime_UTR 55.0%
! ACACCGCGATCATGGTGTTG+TGG + chr8_2:47027685-47027704 Msa1251240:exon 55.0%
!! CACCGCGATCATGGTGTTGT+GGG + chr8_2:47027686-47027705 Msa1251240:exon 55.0%
CTCCCACAACACCATGATCG+CGG - chr8_2:47027691-47027710 None:intergenic 55.0%
GGGTGCGAATAACAGGGAAG+TGG - chr8_2:47028496-47028515 None:intergenic 55.0%
CGTTCACGGGTGCGAATAAC+AGG - chr8_2:47028503-47028522 None:intergenic 55.0%
CCGTCAGCTCAGATTCTTGG+TGG + chr8_2:47029071-47029090 Msa1251240:CDS 55.0%
CTTGAGCTAGCACCAGTGGT+GGG - chr8_2:47029101-47029120 None:intergenic 55.0%
TACGCTTGAGCTAGCACCAG+TGG - chr8_2:47029105-47029124 None:intergenic 55.0%
! GCAATGGTGGGGAAAGGCTT+TGG - chr8_2:47029278-47029297 None:intergenic 55.0%
TTGCGACCACCTAATCCTGC+TGG + chr8_2:47029305-47029324 Msa1251240:CDS 55.0%
TGCGACCACCTAATCCTGCT+GGG + chr8_2:47029306-47029325 Msa1251240:CDS 55.0%
GTCATCCCAGCAGGATTAGG+TGG - chr8_2:47029314-47029333 None:intergenic 55.0%
GTGGTCATCCCAGCAGGATT+AGG - chr8_2:47029317-47029336 None:intergenic 55.0%
CACAATGCTCGTTGGTCAGG+CGG + chr8_2:47029386-47029405 Msa1251240:CDS 55.0%
! CTTGGTGGATCACCCACCAC+TGG + chr8_2:47029086-47029105 Msa1251240:CDS 60.0%
GCTTGAGCTAGCACCAGTGG+TGG - chr8_2:47029102-47029121 None:intergenic 60.0%
! CGCCTGACCAACGAGCATTG+TGG - chr8_2:47029388-47029407 None:intergenic 60.0%
! CTTGGTGGATCACCCACCAC+TGG + chr8_2:47029086-47029105 Msa1251240:CDS 60.0%
GCTTGAGCTAGCACCAGTGG+TGG - chr8_2:47029102-47029121 None:intergenic 60.0%
! CGCCTGACCAACGAGCATTG+TGG - chr8_2:47029388-47029407 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 47027521 47029732 47027521 ID=Msa1251240;Name=Msa1251240
chr8_2 mRNA 47027521 47029732 47027521 ID=Msa1251240-mRNA-1;Parent=Msa1251240;Name=Msa1251240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=175|1|1|1|1|1|2|278|210
chr8_2 exon 47027521 47027826 47027521 ID=Msa1251240-mRNA-1:exon:14255;Parent=Msa1251240-mRNA-1
chr8_2 exon 47028953 47029732 47028953 ID=Msa1251240-mRNA-1:exon:14256;Parent=Msa1251240-mRNA-1
chr8_2 five_prime_UTR 47027521 47027695 47027521 ID=Msa1251240-mRNA-1:five_prime_utr;Parent=Msa1251240-mRNA-1
chr8_2 CDS 47027696 47027826 47027696 ID=Msa1251240-mRNA-1:cds;Parent=Msa1251240-mRNA-1
chr8_2 CDS 47028953 47029454 47028953 ID=Msa1251240-mRNA-1:cds;Parent=Msa1251240-mRNA-1
chr8_2 three_prime_UTR 47029455 47029732 47029455 ID=Msa1251240-mRNA-1:three_prime_utr;Parent=Msa1251240-mRNA-1
Gene Sequence

>Msa1251240

ATGGTGTTGTGGGAGATTGCACTTGGAACTGCATATTTTTTGGGTCTAAAGCGAACTTACAGACACTCTCTAAGGATTCAACGTAGGCTCATTACCCGTAATCATCCTCAGACCCGTCAATTTCTTCACCGAAGAACGCGTTCTGTATTTGATGTGGCAATCAAAGTTCATCAGAAAATTCAAGAAAGGGACACAGAAGTGGGTCGGAATCTCGGAAACTTATTGCTGCGATGGCTTAATAAAATGAAACCGTCAGCTCAGATTCTTGGTGGATCACCCACCACTGGTGCTAGCTCAAGCGTAATGACAAAGCTTCAAGATGGCTCCTCCAACCTCAAAAGACCTAGTTACTATGCTTTATTCAAGAGCTCCAACCTCAAAAGACCTAGTTACTATGCTTTATTCAAGAGGGGATCGAATAAGCGGTTATTTACGCCATCATCAAGTATATGGCCAAAGCCTTTCCCCACCATTGCAAGTATTTTGCGACCACCTAATCCTGCTGGGATGACCACTCATTATCGGAACCTCAGTTACTATCCTCTTGATGCTTTTCGATCAAACCACAATGCTCGTTGGTCAGGCGGTGTTATTAGGAAAGACATCCTGCAATGGTTGATGCTGCAAAACTAA
Protein sequence

>Msa1251240

MVLWEIALGTAYFLGLKRTYRHSLRIQRRLITRNHPQTRQFLHRRTRSVFDVAIKVHQKIQERDTEVGRNLGNLLLRWLNKMKPSAQILGGSPTTGASSSVMTKLQDGSSNLKRPSYYALFKSSNLKRPSYYALFKRGSNKRLFTPSSSIWPKPFPTIASILRPPNPAGMTTHYRNLSYYPLDAFRSNHNARWSGGVIRKDILQWLMLQN*