AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1268680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1268680 MtrunA17_Chr3g0131151 89.617 183 4 1 1 168 1 183 1.12e-108 337
Msa1268680 MtrunA17_Chr3g0131151 97.297 37 1 0 188 224 285 321 2.21e-16 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1268680 AT5G23630.1 65.574 183 48 1 1 168 1 183 1.30e-77 253
Msa1268680 AT5G23630.1 75.000 40 10 0 185 224 283 322 4.25e-12 65.5

Find 55 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCAGACATGCTTATTT+TGG 0.221087 8_3:-1781986 Msa1268680:CDS
TTCTTCATTAGCTGTGGATT+TGG 0.298327 8_3:-1782954 Msa1268680:CDS
GTGATTAGGGTTTGTAGATC+TGG 0.330963 8_3:+1785954 None:intergenic
ATCGATTAAGAGTGGTGATT+AGG 0.344543 8_3:+1785940 None:intergenic
TCGATTAAGAGTGGTGATTA+GGG 0.355394 8_3:+1785941 None:intergenic
TACCCTACCAAGGAAACATT+TGG 0.364945 8_3:-1782857 Msa1268680:CDS
CCTGGAGATGTTGTCTCTAT+AGG 0.374029 8_3:-1782044 Msa1268680:CDS
CTTTGCTGCTACTGAGAAAT+GGG 0.376419 8_3:-1782792 Msa1268680:CDS
TCTTTGCTGCTACTGAGAAA+TGG 0.382427 8_3:-1782793 Msa1268680:CDS
TATCCAAATGTTTCCTTGGT+AGG 0.414685 8_3:+1782854 None:intergenic
TTTCGCCGGAGTTATCTTGC+AGG 0.415946 8_3:+1785547 None:intergenic
TCGTCTGTCTCTTCACTGCT+TGG 0.430171 8_3:-1785709 Msa1268680:CDS
CTCAGTAGCAGCAAAGACTT+TGG 0.432265 8_3:+1782799 None:intergenic
ATCCAAATGTTTCCTTGGTA+GGG 0.444882 8_3:+1782855 None:intergenic
GCCATGGCAACTGCTTCTTC+CGG 0.448621 8_3:+1785849 None:intergenic
AGCAGCAAAGACTTTGGCTT+CGG 0.451999 8_3:+1782805 None:intergenic
TTGGTGAATGTGAAGAGGTT+GGG 0.454013 8_3:+1785987 None:intergenic
ATAATATCCAAATGTTTCCT+TGG 0.465319 8_3:+1782850 None:intergenic
TGCAGGTTCTTCATTAGCTG+TGG 0.470293 8_3:-1782960 Msa1268680:intron
TCAGGTTAAGAATATTGATC+AGG 0.472296 8_3:-1785576 Msa1268680:intron
ACTACAAGGCGAATCATAGT+TGG 0.485273 8_3:-1786061 Msa1268680:five_prime_UTR
TTTCGTTTATTCGAAGGAGA+AGG 0.500968 8_3:-1782900 Msa1268680:CDS
ACAATGTTTCGTTTATTCGA+AGG 0.510158 8_3:-1782906 Msa1268680:CDS
TTTGGTGAATGTGAAGAGGT+TGG 0.511089 8_3:+1785986 None:intergenic
AGACAACATCTCCAGGCAAA+AGG 0.518593 8_3:+1782051 None:intergenic
AGGTCGCTCTGTGGTCAGAA+TGG 0.518779 8_3:-1782024 Msa1268680:CDS
AGGGTAAGAAAGTTTGCAAA+AGG 0.521237 8_3:+1782874 None:intergenic
TTCGTTTATTCGAAGGAGAA+GGG 0.527342 8_3:-1782899 Msa1268680:CDS
CAGGCGAGTCTACTCCTCAA+TGG 0.528010 8_3:-1781930 Msa1268680:CDS
CTACAAGGCGAATCATAGTT+GGG 0.546331 8_3:-1786060 Msa1268680:five_prime_UTR
CGCAAAGCATTTGAAATCAA+CGG 0.561460 8_3:+1785685 None:intergenic
CAAACTTTCTTACCCTACCA+AGG 0.565016 8_3:-1782867 Msa1268680:CDS
GGATATTATGTCAAGAGTAG+TGG 0.570699 8_3:-1782836 Msa1268680:CDS
GCCAAAATAAGCATGTCTGC+AGG 0.576308 8_3:+1781985 None:intergenic
GTGAATGAAGCTATTCTCAC+AGG 0.578492 8_3:-1781949 Msa1268680:CDS
TCGTTTATTCGAAGGAGAAG+GGG 0.582425 8_3:-1782898 Msa1268680:CDS
AAGAGAAACACCTTCCATTG+AGG 0.582504 8_3:+1781916 None:intergenic
TTTGAACCACAAAATTTCGC+CGG 0.584168 8_3:+1785533 None:intergenic
CCTATAGAGACAACATCTCC+AGG 0.586206 8_3:+1782044 None:intergenic
TAGACAAAGTTGATCTACTC+CGG 0.590757 8_3:-1785868 Msa1268680:CDS
TGGGTTTAACGCCACGCTAG+TGG 0.593151 8_3:+1786006 None:intergenic
TATGTCAAGAGTAGTGGTCA+TGG 0.596270 8_3:-1782830 Msa1268680:CDS
TCCGGAAGAAGCAGTTGCCA+TGG 0.599657 8_3:-1785850 Msa1268680:CDS
CGAGTCTACTCCTCAATGGA+AGG 0.601332 8_3:-1781926 None:intergenic
ACAAATCCTCGCCACTAGCG+TGG 0.609403 8_3:-1786017 Msa1268680:five_prime_UTR
AACGGAAAAGAGAGACTACA+AGG 0.614754 8_3:-1786075 Msa1268680:five_prime_UTR
TTAACGCCACGCTAGTGGCG+AGG 0.615274 8_3:+1786011 None:intergenic
ATGCTTTGCGTATTACAGCA+AGG 0.619929 8_3:-1785672 Msa1268680:intron
GCGCCGTATAAAATAGCGAA+AGG 0.633523 8_3:+1785812 None:intergenic
GCATCGACGAAATCGAGACT+AGG 0.658093 8_3:+1785770 None:intergenic
TGATTCCTGCAAGATAACTC+CGG 0.669263 8_3:-1785552 Msa1268680:CDS
TTGCTGCTACTGAGAAATGG+GGG 0.674653 8_3:-1782790 Msa1268680:CDS
TTTGCTGCTACTGAGAAATG+GGG 0.684736 8_3:-1782791 Msa1268680:CDS
GCTCTGTGGTCAGAATGGAG+AGG 0.727356 8_3:-1782019 Msa1268680:CDS
TGTCTCTATAGGTCGCTCTG+TGG 0.736469 8_3:-1782033 Msa1268680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTTATATTAATATA+TGG + chr8_3:1784482-1784501 None:intergenic 0.0%
!! ATTTAGAAAAAAATAGAATT+TGG + chr8_3:1783833-1783852 None:intergenic 10.0%
!!! TTTAGTATTTTAATAAGTTG+CGG - chr8_3:1782727-1782746 Msa1268680:intron 15.0%
!!! TTTCTTTTGAGTTGTTTAAT+TGG - chr8_3:1782593-1782612 Msa1268680:intron 20.0%
!! GATTTGAAAAATTCAATAGA+AGG - chr8_3:1783246-1783265 Msa1268680:intron 20.0%
!! AAAAATTCAATAGAAGGATT+TGG - chr8_3:1783252-1783271 Msa1268680:intron 20.0%
!! GAAACTATATTGAAAATTCA+GGG - chr8_3:1783294-1783313 Msa1268680:intron 20.0%
!! TCAAAAAAATCCAAAACAAA+CGG + chr8_3:1783677-1783696 None:intergenic 20.0%
!!! AAATGATTTTTCTAGCAAAA+TGG + chr8_3:1783773-1783792 None:intergenic 20.0%
!!! TGAAATAGATTTTCATTTTG+AGG + chr8_3:1783800-1783819 None:intergenic 20.0%
!!! TCTATTTTTTTCTAAATGCA+AGG - chr8_3:1783836-1783855 Msa1268680:intron 20.0%
!!! ATGTAATTTGTCCAAAAAAA+TGG + chr8_3:1784425-1784444 None:intergenic 20.0%
!!! TTCTTTATGATTTTGTGTTT+AGG - chr8_3:1784723-1784742 Msa1268680:intron 20.0%
!! TTTACCTGAAATACAAAAAA+AGG + chr8_3:1785577-1785596 None:intergenic 20.0%
!!! TTTGTATTTGTGTTGATTTT+AGG - chr8_3:1785923-1785942 Msa1268680:five_prime_UTR 20.0%
!!! TTTCCTTTCGCTATTTTATA+CGG - chr8_3:1782206-1782225 Msa1268680:intron 25.0%
!! TGATCAAGTTTGTTCAATTT+CGG - chr8_3:1782393-1782412 Msa1268680:intron 25.0%
! TTTGAATAGAAGCTAACAAT+TGG - chr8_3:1782762-1782781 Msa1268680:intron 25.0%
!!! TTGTTCAACTCGTTTTTTTA+CGG + chr8_3:1782959-1782978 None:intergenic 25.0%
! TAAAATATAAACTGTTCCAG+AGG + chr8_3:1783142-1783161 None:intergenic 25.0%
!!! AGTATTTTGGATCTTATAGT+TGG - chr8_3:1783183-1783202 Msa1268680:intron 25.0%
! GGAAACTATATTGAAAATTC+AGG - chr8_3:1783293-1783312 Msa1268680:intron 25.0%
! AAGTCTATTACTAGTGTTTA+CGG - chr8_3:1783406-1783425 Msa1268680:intron 25.0%
! AATTTATACTAGTCCTCTTT+AGG + chr8_3:1783588-1783607 None:intergenic 25.0%
! TAAGAATTGCTCAAAAATGA+TGG + chr8_3:1783619-1783638 None:intergenic 25.0%
! AAAAACTAATGCCTGATTTA+GGG + chr8_3:1784013-1784032 None:intergenic 25.0%
! AAAAAACTAATGCCTGATTT+AGG + chr8_3:1784014-1784033 None:intergenic 25.0%
!!! TCAGGCATTAGTTTTTTTTT+TGG - chr8_3:1784017-1784036 Msa1268680:intron 25.0%
! TATGTCTTAGATTAAGACTT+GGG + chr8_3:1784574-1784593 None:intergenic 25.0%
!!! ATTTTGTTGGTATTTGGAAT+TGG + chr8_3:1784600-1784619 None:intergenic 25.0%
! AATCTTTGCTTGATTTATGT+TGG - chr8_3:1784749-1784768 Msa1268680:intron 25.0%
!! TATGTTGGAAAATTTTCTTG+TGG - chr8_3:1784764-1784783 Msa1268680:intron 25.0%
! ATAATATCCAAATGTTTCCT+TGG + chr8_3:1785174-1785193 None:intergenic 25.0%
! ACATTATAATGTGTACTGAA+GGG + chr8_3:1785316-1785335 None:intergenic 25.0%
!!! TTCATCAATTTCTGAAATGT+TGG + chr8_3:1785538-1785557 None:intergenic 25.0%
!! TTTTCTCTGTTAATTGTGTT+TGG - chr8_3:1785610-1785629 Msa1268680:intron 25.0%
!!! TACTATAGTTTGTTCACTTT+AGG - chr8_3:1785723-1785742 Msa1268680:CDS 25.0%
!!! TATTTGTGTTGATTTTAGGT+GGG - chr8_3:1785927-1785946 Msa1268680:five_prime_UTR 25.0%
GCAGACTGAAAGTAAAAAAA+CGG - chr8_3:1781928-1781947 Msa1268680:CDS 30.0%
!!! AATTTTTGGTGAATGTGAAG+AGG + chr8_3:1782042-1782061 None:intergenic 30.0%
! TAGATCTGGTTGAGAATTTT+TGG + chr8_3:1782056-1782075 None:intergenic 30.0%
!! TTTTGAGAATCGATTAAGAG+TGG + chr8_3:1782092-1782111 None:intergenic 30.0%
! TTAATGTCAACTTTTCACGT+CGG - chr8_3:1782119-1782138 Msa1268680:intron 30.0%
TCAGGTTAAGAATATTGATC+AGG - chr8_3:1782445-1782464 Msa1268680:intron 30.0%
!! ATTGAGTTGAAATTGAAGCT+AGG - chr8_3:1782568-1782587 Msa1268680:intron 30.0%
! CTGATTGAGATAGTATTGAA+AGG - chr8_3:1782669-1782688 Msa1268680:intron 30.0%
ATGTTAAAATCACTTCTGGA+GGG - chr8_3:1782988-1783007 Msa1268680:intron 30.0%
!!! TTTTTTGACACATCCAAAAG+TGG - chr8_3:1783097-1783116 Msa1268680:intron 30.0%
!! ATACTCTCTAAAACAGGATT+GGG + chr8_3:1783168-1783187 None:intergenic 30.0%
!! AATACTCTCTAAAACAGGAT+TGG + chr8_3:1783169-1783188 None:intergenic 30.0%
!!! TCCTGTTTTAGAGAGTATTT+TGG - chr8_3:1783170-1783189 Msa1268680:intron 30.0%
!! TCCAAAATACTCTCTAAAAC+AGG + chr8_3:1783174-1783193 None:intergenic 30.0%
CAAACGAAGATCCTAAAATA+TGG + chr8_3:1783227-1783246 None:intergenic 30.0%
GATTTGGATACAGGATTTAA+GGG - chr8_3:1783268-1783287 Msa1268680:intron 30.0%
ATTTGGATACAGGATTTAAG+GGG - chr8_3:1783269-1783288 Msa1268680:intron 30.0%
ACTGAGCTGCAAAATTATTA+TGG + chr8_3:1783368-1783387 None:intergenic 30.0%
ATTCTTAATCGCCAATTTAG+AGG - chr8_3:1783632-1783651 Msa1268680:intron 30.0%
! GAAAGCTAGATTCAGTTTTA+AGG - chr8_3:1784086-1784105 Msa1268680:intron 30.0%
! TTTGTTGCTACCTCTTTTAT+TGG - chr8_3:1784116-1784135 Msa1268680:intron 30.0%
GAACACAAAAAAGCAAAGAT+CGG + chr8_3:1784147-1784166 None:intergenic 30.0%
CAATAAACTAATGGAAGATG+AGG + chr8_3:1784273-1784292 None:intergenic 30.0%
CGATGTTGACAATAAACTAA+TGG + chr8_3:1784282-1784301 None:intergenic 30.0%
GTATGTCTTAGATTAAGACT+TGG + chr8_3:1784575-1784594 None:intergenic 30.0%
! CCTTGAATTTTGTTGGTATT+TGG + chr8_3:1784606-1784625 None:intergenic 30.0%
CCAAATACCAACAAAATTCA+AGG - chr8_3:1784603-1784622 Msa1268680:intron 30.0%
TACCAACAAAATTCAAGGAT+TGG - chr8_3:1784608-1784627 Msa1268680:intron 30.0%
AAGAACCTATGAAGAAAGAA+CGG + chr8_3:1784999-1785018 None:intergenic 30.0%
CACAAGAAAATACTTAACCA+TGG + chr8_3:1785036-1785055 None:intergenic 30.0%
ACAATGTTTCGTTTATTCGA+AGG - chr8_3:1785115-1785134 Msa1268680:intron 30.0%
! ATGATGATGATCGAAGAAAA+AGG + chr8_3:1785272-1785291 None:intergenic 30.0%
CACATTATAATGTGTACTGA+AGG + chr8_3:1785317-1785336 None:intergenic 30.0%
ATCACAAATACCAAAGTAAC+GGG + chr8_3:1785400-1785419 None:intergenic 30.0%
CATCACAAATACCAAAGTAA+CGG + chr8_3:1785401-1785420 None:intergenic 30.0%
!!! CTTTCTCAAAACGTGTTTTT+TGG - chr8_3:1785439-1785458 Msa1268680:intron 30.0%
!!! GGAACCTTTTTTTGTATTTC+AGG - chr8_3:1785570-1785589 Msa1268680:CDS 30.0%
CTTCATTCTAACTTTCGATT+TGG - chr8_3:1785634-1785653 Msa1268680:intron 30.0%
!!! GTATTTGTGTTGATTTTAGG+TGG - chr8_3:1785926-1785945 Msa1268680:five_prime_UTR 30.0%
TCGATTAAGAGTGGTGATTA+GGG + chr8_3:1782083-1782102 None:intergenic 35.0%
ATCGATTAAGAGTGGTGATT+AGG + chr8_3:1782084-1782103 None:intergenic 35.0%
! TAGACAAAGTTGATCTACTC+CGG - chr8_3:1782153-1782172 Msa1268680:intron 35.0%
! GTCGATGCTTGTATTGTTTT+AGG - chr8_3:1782266-1782285 Msa1268680:intron 35.0%
! CGCAAAGCATTTGAAATCAA+CGG + chr8_3:1782339-1782358 None:intergenic 35.0%
TTTGAACCACAAAATTTCGC+CGG + chr8_3:1782491-1782510 None:intergenic 35.0%
TTAGCTTCTATTCAAACGTG+CGG + chr8_3:1782758-1782777 None:intergenic 35.0%
AAGCTAACAATTGGAGCTTT+AGG - chr8_3:1782771-1782790 Msa1268680:intron 35.0%
CTGCATGTTAAAATCACTTC+TGG - chr8_3:1782984-1783003 Msa1268680:intron 35.0%
CATGTTAAAATCACTTCTGG+AGG - chr8_3:1782987-1783006 Msa1268680:intron 35.0%
!! TGTAAGTTTGGTTCCACTTT+TGG + chr8_3:1783113-1783132 None:intergenic 35.0%
!! GGATCTTATAGTTGGTAAAG+AGG - chr8_3:1783191-1783210 Msa1268680:intron 35.0%
! GCAAGTACACACCATATTTT+AGG - chr8_3:1783213-1783232 Msa1268680:intron 35.0%
CAATAGAAGGATTTGGATAC+AGG - chr8_3:1783259-1783278 Msa1268680:intron 35.0%
GGATTTGGATACAGGATTTA+AGG - chr8_3:1783267-1783286 Msa1268680:intron 35.0%
TGAAATGGCTTAAAAAAGGC+AGG - chr8_3:1783514-1783533 Msa1268680:intron 35.0%
GAAATGGCTTAAAAAAGGCA+GGG - chr8_3:1783515-1783534 Msa1268680:intron 35.0%
AAATGGCTTAAAAAAGGCAG+GGG - chr8_3:1783516-1783535 Msa1268680:intron 35.0%
TCCTCTCAAAACCTCTAAAT+TGG + chr8_3:1783646-1783665 None:intergenic 35.0%
ATCGGTCTATCCAATAAAAG+AGG + chr8_3:1784129-1784148 None:intergenic 35.0%
GGTTTCATTATTGGTATGCT+AGG - chr8_3:1784191-1784210 Msa1268680:intron 35.0%
GTTTATTGTCAACATCGTAG+AGG - chr8_3:1784285-1784304 Msa1268680:intron 35.0%
TAGTTGTGAAGTCTTTCCTT+CGG - chr8_3:1784326-1784345 Msa1268680:intron 35.0%
TCCAAAAAAATGGTCAATCC+CGG + chr8_3:1784415-1784434 None:intergenic 35.0%
! AACAAAATTCAAGGATTGGC+CGG - chr8_3:1784612-1784631 Msa1268680:intron 35.0%
ATGCTCATTGTGCATAATTC+TGG - chr8_3:1784807-1784826 Msa1268680:intron 35.0%
TTATCCCGTTCTTTCTTCAT+AGG - chr8_3:1784991-1785010 Msa1268680:intron 35.0%
AGAACCTATGAAGAAAGAAC+GGG + chr8_3:1784998-1785017 None:intergenic 35.0%
TTCTTCATTAGCTGTGGATT+TGG - chr8_3:1785067-1785086 Msa1268680:intron 35.0%
TTTCGTTTATTCGAAGGAGA+AGG - chr8_3:1785121-1785140 Msa1268680:intron 35.0%
TTCGTTTATTCGAAGGAGAA+GGG - chr8_3:1785122-1785141 Msa1268680:intron 35.0%
AGGGTAAGAAAGTTTGCAAA+AGG + chr8_3:1785150-1785169 None:intergenic 35.0%
ATCCAAATGTTTCCTTGGTA+GGG + chr8_3:1785169-1785188 None:intergenic 35.0%
TATCCAAATGTTTCCTTGGT+AGG + chr8_3:1785170-1785189 None:intergenic 35.0%
GGATATTATGTCAAGAGTAG+TGG - chr8_3:1785185-1785204 Msa1268680:intron 35.0%
TTTGAGAAAGACAGAAGGAA+CGG + chr8_3:1785429-1785448 None:intergenic 35.0%
!!! AACGTGTTTTTTGGCTTCTT+AGG - chr8_3:1785448-1785467 Msa1268680:intron 35.0%
TTTGATCTCTAATGTTCTGC+TGG - chr8_3:1785484-1785503 Msa1268680:intron 35.0%
!! AATTTCTGAAATGTTGGCTG+AGG + chr8_3:1785532-1785551 None:intergenic 35.0%
!! TTCTTGCTTTTATCATGACC+AGG - chr8_3:1785662-1785681 Msa1268680:intron 35.0%
TCTGGTGTTTGGATGAATAT+TGG - chr8_3:1785700-1785719 Msa1268680:CDS 35.0%
TGTTTATGTTGAGTCAACGA+TGG - chr8_3:1785751-1785770 Msa1268680:CDS 35.0%
AGTGGATAATCAGATCGTAA+TGG - chr8_3:1785817-1785836 Msa1268680:CDS 35.0%
!! ATCATTAGCTTGCTCATACT+TGG - chr8_3:1785868-1785887 Msa1268680:CDS 35.0%
!! TCATTAGCTTGCTCATACTT+GGG - chr8_3:1785869-1785888 Msa1268680:CDS 35.0%
TTAGGTGGGTAAAAAATCTC+TGG - chr8_3:1785941-1785960 Msa1268680:five_prime_UTR 35.0%
AACGGAAAAGAGAGACTACA+AGG - chr8_3:1781946-1781965 Msa1268680:CDS 40.0%
ACTACAAGGCGAATCATAGT+TGG - chr8_3:1781960-1781979 Msa1268680:CDS 40.0%
CTACAAGGCGAATCATAGTT+GGG - chr8_3:1781961-1781980 Msa1268680:CDS 40.0%
TTGGTGAATGTGAAGAGGTT+GGG + chr8_3:1782037-1782056 None:intergenic 40.0%
TTTGGTGAATGTGAAGAGGT+TGG + chr8_3:1782038-1782057 None:intergenic 40.0%
GTGATTAGGGTTTGTAGATC+TGG + chr8_3:1782070-1782089 None:intergenic 40.0%
ATGCTTTGCGTATTACAGCA+AGG - chr8_3:1782349-1782368 Msa1268680:intron 40.0%
ATAATCGCGTTTGTGTGTTC+AGG - chr8_3:1782427-1782446 Msa1268680:intron 40.0%
TGATTCCTGCAAGATAACTC+CGG - chr8_3:1782469-1782488 Msa1268680:intron 40.0%
! ATAACTCCGGCGAAATTTTG+TGG - chr8_3:1782482-1782501 Msa1268680:intron 40.0%
CAGAGGACTAAGTGTAAGTT+TGG + chr8_3:1783125-1783144 None:intergenic 40.0%
CAAACTTACACTTAGTCCTC+TGG - chr8_3:1783123-1783142 Msa1268680:intron 40.0%
CAGCTCAGTGCAATGAAAAT+TGG - chr8_3:1783379-1783398 Msa1268680:intron 40.0%
CGTGCATGGAATATCCTTTA+AGG - chr8_3:1783460-1783479 Msa1268680:intron 40.0%
CGGGTGAAATGGCTTAAAAA+AGG - chr8_3:1783510-1783529 Msa1268680:intron 40.0%
!! TGTGTTTCTAGCACCTAAAG+AGG - chr8_3:1783572-1783591 Msa1268680:intron 40.0%
!!! GCCAATTTAGAGGTTTTGAG+AGG - chr8_3:1783642-1783661 Msa1268680:intron 40.0%
!!! TAGAGGTTTTGAGAGGAGTA+AGG - chr8_3:1783649-1783668 Msa1268680:intron 40.0%
!!! AGAGGTTTTGAGAGGAGTAA+GGG - chr8_3:1783650-1783669 Msa1268680:intron 40.0%
ATGCAAGGAACTCAAGTGTA+AGG - chr8_3:1783851-1783870 Msa1268680:intron 40.0%
TGCAAGGAACTCAAGTGTAA+GGG - chr8_3:1783852-1783871 Msa1268680:intron 40.0%
AAACAAACGCACCCTAAATC+AGG - chr8_3:1783999-1784018 Msa1268680:intron 40.0%
CTAGGTAGTCAAGTCAATTC+TGG - chr8_3:1784209-1784228 Msa1268680:intron 40.0%
GTCAAGTCAATTCTGGATAG+CGG - chr8_3:1784216-1784235 Msa1268680:intron 40.0%
TATTGTCAACATCGTAGAGG+TGG - chr8_3:1784288-1784307 Msa1268680:intron 40.0%
AGTTGTGAAGTCTTTCCTTC+GGG - chr8_3:1784327-1784346 Msa1268680:intron 40.0%
AGATCAGACAAATAACCCGA+AGG + chr8_3:1784345-1784364 None:intergenic 40.0%
! GGCCAATCCTTGAATTTTGT+TGG + chr8_3:1784613-1784632 None:intergenic 40.0%
!! ACAAAATTCAAGGATTGGCC+GGG - chr8_3:1784613-1784632 Msa1268680:intron 40.0%
GCAAAAGAGTCAGTAAGACA+AGG + chr8_3:1784949-1784968 None:intergenic 40.0%
GAAAGAACGGGATAAGAGAA+GGG + chr8_3:1784986-1785005 None:intergenic 40.0%
AGAAAGAACGGGATAAGAGA+AGG + chr8_3:1784987-1785006 None:intergenic 40.0%
CTTGCTTTGATTGTCTACCA+TGG - chr8_3:1785016-1785035 Msa1268680:intron 40.0%
! TATTTTCTTGTGCAGTCTGC+AGG - chr8_3:1785044-1785063 Msa1268680:intron 40.0%
TCGTTTATTCGAAGGAGAAG+GGG - chr8_3:1785123-1785142 Msa1268680:intron 40.0%
CAAACTTTCTTACCCTACCA+AGG - chr8_3:1785154-1785173 Msa1268680:intron 40.0%
TACCCTACCAAGGAAACATT+TGG - chr8_3:1785164-1785183 Msa1268680:intron 40.0%
TATGTCAAGAGTAGTGGTCA+TGG - chr8_3:1785191-1785210 Msa1268680:intron 40.0%
TCTTTGCTGCTACTGAGAAA+TGG - chr8_3:1785228-1785247 Msa1268680:intron 40.0%
CTTTGCTGCTACTGAGAAAT+GGG - chr8_3:1785229-1785248 Msa1268680:intron 40.0%
TTTGCTGCTACTGAGAAATG+GGG - chr8_3:1785230-1785249 Msa1268680:intron 40.0%
TGAAGGGAAATAGATGCATG+TGG + chr8_3:1785300-1785319 None:intergenic 40.0%
TAGATGCAAACCCGTTACTT+TGG - chr8_3:1785387-1785406 Msa1268680:intron 40.0%
!!! CACGTTTTGAGAAAGACAGA+AGG + chr8_3:1785434-1785453 None:intergenic 40.0%
!! ATTGATGAAAGAGCACTGCA+TGG - chr8_3:1785549-1785568 Msa1268680:CDS 40.0%
AGTCAACGATGGCAAAAAGT+CGG - chr8_3:1785762-1785781 Msa1268680:CDS 40.0%
TGAGTTAAGACGTGTTAGAG+TGG - chr8_3:1785799-1785818 Msa1268680:CDS 40.0%
ATCGTAATGGTACATCGTTG+TGG - chr8_3:1785830-1785849 Msa1268680:CDS 40.0%
!! CATTAGCTTGCTCATACTTG+GGG - chr8_3:1785870-1785889 Msa1268680:CDS 40.0%
!! ATTAGCTTGCTCATACTTGG+GGG - chr8_3:1785871-1785890 Msa1268680:CDS 40.0%
! ACCTGCAGACATGCTTATTT+TGG - chr8_3:1786035-1786054 Msa1268680:five_prime_UTR 40.0%
GTGAATGAAGCTATTCTCAC+AGG - chr8_3:1786072-1786091 Msa1268680:five_prime_UTR 40.0%
! ATGTCAACTTTTCACGTCGG+CGG - chr8_3:1782122-1782141 Msa1268680:intron 45.0%
GCGCCGTATAAAATAGCGAA+AGG + chr8_3:1782212-1782231 None:intergenic 45.0%
!! AAGTTGTGCCGCTTAATTCG+CGG - chr8_3:1782513-1782532 Msa1268680:intron 45.0%
! ACCGATTTCCGCGAATTAAG+CGG + chr8_3:1782524-1782543 None:intergenic 45.0%
TGGATACAGGATTTAAGGGG+AGG - chr8_3:1783272-1783291 Msa1268680:intron 45.0%
TATTCCATGCACGAACTCAC+TGG + chr8_3:1783453-1783472 None:intergenic 45.0%
TAACCTACCTTGCCTCAACT+GGG + chr8_3:1783489-1783508 None:intergenic 45.0%
ATAACCTACCTTGCCTCAAC+TGG + chr8_3:1783490-1783509 None:intergenic 45.0%
GTTGAGGCAAGGTAGGTTAT+CGG - chr8_3:1783490-1783509 Msa1268680:intron 45.0%
TTGAGGCAAGGTAGGTTATC+GGG - chr8_3:1783491-1783510 Msa1268680:intron 45.0%
AGGTAGGTTATCGGGTGAAA+TGG - chr8_3:1783499-1783518 Msa1268680:intron 45.0%
GCTTAAAAAAGGCAGGGGTT+GGG - chr8_3:1783521-1783540 Msa1268680:intron 45.0%
CTTAAAAAAGGCAGGGGTTG+GGG - chr8_3:1783522-1783541 Msa1268680:intron 45.0%
TTAAAAAAGGCAGGGGTTGG+GGG - chr8_3:1783523-1783542 Msa1268680:intron 45.0%
! GAGTAAGGGTCCGTTTGTTT+TGG - chr8_3:1783664-1783683 Msa1268680:intron 45.0%
AGCTTTCCTTTCCGAACCAT+AGG + chr8_3:1784073-1784092 None:intergenic 45.0%
ATAATGAAACCCTGCCATGG+TGG + chr8_3:1784182-1784201 None:intergenic 45.0%
CCAATAATGAAACCCTGCCA+TGG + chr8_3:1784185-1784204 None:intergenic 45.0%
CCATGGCAGGGTTTCATTAT+TGG - chr8_3:1784182-1784201 Msa1268680:intron 45.0%
!!! GTTCATTGCTCGCCACTTTT+GGG + chr8_3:1784251-1784270 None:intergenic 45.0%
AGAACACGGCTTAAAGTAGC+AGG - chr8_3:1784385-1784404 Msa1268680:intron 45.0%
!!! GCCGGGATTGACCATTTTTT+TGG - chr8_3:1784411-1784430 Msa1268680:intron 45.0%
ATGCCAACTGTGTACAATCC+AGG + chr8_3:1784675-1784694 None:intergenic 45.0%
GTAAACAAAGAAGCAGCTGC+TGG + chr8_3:1784919-1784938 None:intergenic 45.0%
TGCAGGTTCTTCATTAGCTG+TGG - chr8_3:1785061-1785080 Msa1268680:intron 45.0%
! AGCAGCAAAGACTTTGGCTT+CGG + chr8_3:1785219-1785238 None:intergenic 45.0%
CTCAGTAGCAGCAAAGACTT+TGG + chr8_3:1785225-1785244 None:intergenic 45.0%
TTGCTGCTACTGAGAAATGG+GGG - chr8_3:1785231-1785250 Msa1268680:intron 45.0%
!! TTAGCTTGCTCATACTTGGG+GGG - chr8_3:1785872-1785891 Msa1268680:CDS 45.0%
AGACAACATCTCCAGGCAAA+AGG + chr8_3:1785973-1785992 None:intergenic 45.0%
CCTATAGAGACAACATCTCC+AGG + chr8_3:1785980-1785999 None:intergenic 45.0%
CCTGGAGATGTTGTCTCTAT+AGG - chr8_3:1785977-1785996 Msa1268680:five_prime_UTR 45.0%
GCCAAAATAAGCATGTCTGC+AGG + chr8_3:1786039-1786058 None:intergenic 45.0%
!!! GCAGACATGCTTATTTTGGC+TGG - chr8_3:1786039-1786058 Msa1268680:five_prime_UTR 45.0%
!!! ATAATTTATTTTGAAATATT+TGG + chr8_3:1783026-1783045 None:intergenic 5.0%
! AACTTTTCACGTCGGCGGTA+AGG - chr8_3:1782127-1782146 Msa1268680:intron 50.0%
GGAAAAACGTCTAAGCGCCA+TGG + chr8_3:1782191-1782210 None:intergenic 50.0%
!! TCGCTATTTTATACGGCGCG+TGG - chr8_3:1782213-1782232 Msa1268680:intron 50.0%
! GCATCGACGAAATCGAGACT+AGG + chr8_3:1782254-1782273 None:intergenic 50.0%
TCGTCTGTCTCTTCACTGCT+TGG - chr8_3:1782312-1782331 Msa1268680:intron 50.0%
TTTCGCCGGAGTTATCTTGC+AGG + chr8_3:1782477-1782496 None:intergenic 50.0%
GCCGCTTAATTCGCGGAAAT+CGG - chr8_3:1782520-1782539 Msa1268680:intron 50.0%
!! TTGGAGCTTTAGGAGACCTG+CGG - chr8_3:1782781-1782800 Msa1268680:intron 50.0%
TGGGTTGTCAAACACCCACT+TGG - chr8_3:1782841-1782860 Msa1268680:CDS 50.0%
AACCGAAACACGCACCAAGT+GGG + chr8_3:1782858-1782877 None:intergenic 50.0%
TAACCGAAACACGCACCAAG+TGG + chr8_3:1782859-1782878 None:intergenic 50.0%
TGGTGCGTGTTTCGGTTATG+CGG - chr8_3:1782861-1782880 Msa1268680:CDS 50.0%
GGCTTAAAAAAGGCAGGGGT+TGG - chr8_3:1783520-1783539 Msa1268680:intron 50.0%
GGTGCTAGAAACACACAACG+AGG + chr8_3:1783567-1783586 None:intergenic 50.0%
! TTTGCACTGCATGCACTGAC+TGG - chr8_3:1784043-1784062 Msa1268680:intron 50.0%
!! TTGCACTGCATGCACTGACT+GGG - chr8_3:1784044-1784063 Msa1268680:intron 50.0%
TGACTGGGTTGCCTATGGTT+CGG - chr8_3:1784059-1784078 Msa1268680:intron 50.0%
TCCTAGTATACCAGCCACCA+TGG - chr8_3:1784165-1784184 Msa1268680:intron 50.0%
! GTCAATTCTGGATAGCGGTG+TGG - chr8_3:1784221-1784240 Msa1268680:intron 50.0%
!!! ATTGCTCGCCACTTTTGGGT+CGG + chr8_3:1784247-1784266 None:intergenic 50.0%
!! GGTTCATTGCTCGCCACTTT+TGG + chr8_3:1784252-1784271 None:intergenic 50.0%
AGTGTTTGAGCGCGAGAACA+CGG - chr8_3:1784371-1784390 Msa1268680:intron 50.0%
GCTTAAAGTAGCAGGATCGC+CGG - chr8_3:1784393-1784412 Msa1268680:intron 50.0%
CTTAAAGTAGCAGGATCGCC+GGG - chr8_3:1784394-1784413 Msa1268680:intron 50.0%
ACGCCTGGATTGTACACAGT+TGG - chr8_3:1784669-1784688 Msa1268680:intron 50.0%
! CATGACCAGGTTTTCTGCGT+TGG - chr8_3:1785675-1785694 Msa1268680:CDS 50.0%
AGAGACCAACGCAGAAAACC+TGG + chr8_3:1785683-1785702 None:intergenic 50.0%
!! AGGTTTTCTGCGTTGGTCTC+TGG - chr8_3:1785682-1785701 Msa1268680:CDS 50.0%
CGATGGCAAAAAGTCGGTTG+AGG - chr8_3:1785768-1785787 Msa1268680:CDS 50.0%
! TCTGGTACAGACCTTTTGCC+TGG - chr8_3:1785959-1785978 Msa1268680:five_prime_UTR 50.0%
TGTCTCTATAGGTCGCTCTG+TGG - chr8_3:1785988-1786007 Msa1268680:five_prime_UTR 50.0%
ACAAATCCTCGCCACTAGCG+TGG - chr8_3:1782004-1782023 Msa1268680:CDS 55.0%
TGGGTTTAACGCCACGCTAG+TGG + chr8_3:1782018-1782037 None:intergenic 55.0%
TCCGGAAGAAGCAGTTGCCA+TGG - chr8_3:1782171-1782190 Msa1268680:intron 55.0%
GCCATGGCAACTGCTTCTTC+CGG + chr8_3:1782175-1782194 None:intergenic 55.0%
TTTAGGAGACCTGCGGTGAG+AGG - chr8_3:1782788-1782807 Msa1268680:CDS 55.0%
TTAGGAGACCTGCGGTGAGA+GGG - chr8_3:1782789-1782808 Msa1268680:CDS 55.0%
TGAGAGGGGTCTGAAAGGTG+AGG - chr8_3:1782804-1782823 Msa1268680:CDS 55.0%
AGGGGTCTGAAAGGTGAGGT+CGG - chr8_3:1782808-1782827 Msa1268680:CDS 55.0%
!! CACCCACTTGGTGCGTGTTT+CGG - chr8_3:1782853-1782872 Msa1268680:CDS 55.0%
TGCGTGTTTCGGTTATGCGG+TGG - chr8_3:1782864-1782883 Msa1268680:CDS 55.0%
GCGTGTTTCGGTTATGCGGT+GGG - chr8_3:1782865-1782884 Msa1268680:CDS 55.0%
CATGCCAGTGAGTTCGTGCA+TGG - chr8_3:1783446-1783465 Msa1268680:intron 55.0%
CCTCAACTGGGCAGCCTTAA+AGG + chr8_3:1783477-1783496 None:intergenic 55.0%
!! CCTTTAAGGCTGCCCAGTTG+AGG - chr8_3:1783474-1783493 Msa1268680:intron 55.0%
!! TGCACTGACTGGGTTGCCTA+TGG - chr8_3:1784054-1784073 Msa1268680:intron 55.0%
! GGGTTGCCTATGGTTCGGAA+AGG - chr8_3:1784064-1784083 Msa1268680:intron 55.0%
GCCATGGTGGCTGGTATACT+AGG + chr8_3:1784169-1784188 None:intergenic 55.0%
AGTATACCAGCCACCATGGC+AGG - chr8_3:1784169-1784188 Msa1268680:intron 55.0%
GTATACCAGCCACCATGGCA+GGG - chr8_3:1784170-1784189 Msa1268680:intron 55.0%
! GATTGGCCGGGATCCTTTCT+GGG - chr8_3:1784625-1784644 Msa1268680:intron 55.0%
AGAAGCAGCTGCTGGCAAGT+GGG + chr8_3:1784911-1784930 None:intergenic 55.0%
AAGAAGCAGCTGCTGGCAAG+TGG + chr8_3:1784912-1784931 None:intergenic 55.0%
CTGCTACTGAGAAATGGGGG+CGG - chr8_3:1785234-1785253 Msa1268680:intron 55.0%
AGAAATGGGGGCGGAATGTG+TGG - chr8_3:1785243-1785262 Msa1268680:intron 55.0%
GAAATGGGGGCGGAATGTGT+GGG - chr8_3:1785244-1785263 Msa1268680:intron 55.0%
! CTGCGTTGGTCTCTGGTGTT+TGG - chr8_3:1785689-1785708 Msa1268680:CDS 55.0%
AGGTCGCTCTGTGGTCAGAA+TGG - chr8_3:1785997-1786016 Msa1268680:five_prime_UTR 55.0%
GCTCTGTGGTCAGAATGGAG+AGG - chr8_3:1786002-1786021 Msa1268680:five_prime_UTR 55.0%
CAGGCGAGTCTACTCCTCAA+TGG - chr8_3:1786091-1786110 Msa1268680:five_prime_UTR 55.0%
TTAACGCCACGCTAGTGGCG+AGG + chr8_3:1782013-1782032 None:intergenic 60.0%
TAGGAGACCTGCGGTGAGAG+GGG - chr8_3:1782790-1782809 Msa1268680:CDS 60.0%
TTTCAGACCCCTCTCACCGC+AGG + chr8_3:1782800-1782819 None:intergenic 60.0%
TGCGGTGAGAGGGGTCTGAA+AGG - chr8_3:1782799-1782818 Msa1268680:CDS 60.0%
GGGGTCTGAAAGGTGAGGTC+GGG - chr8_3:1782809-1782828 Msa1268680:CDS 60.0%
GGGTCTGAAAGGTGAGGTCG+GGG - chr8_3:1782810-1782829 Msa1268680:CDS 60.0%
TGAGGTCGGGGTGAGCTACT+GGG - chr8_3:1782822-1782841 Msa1268680:CDS 60.0%
AAGGCTGCCCAGTTGAGGCA+AGG - chr8_3:1783479-1783498 Msa1268680:intron 60.0%
CTGCCCAGTTGAGGCAAGGT+AGG - chr8_3:1783483-1783502 Msa1268680:intron 60.0%
TGAAACCCTGCCATGGTGGC+TGG + chr8_3:1784178-1784197 None:intergenic 60.0%
!! GGATTGGCCGGGATCCTTTC+TGG - chr8_3:1784624-1784643 Msa1268680:intron 60.0%
GAGCAGCCCAGAAAGGATCC+CGG + chr8_3:1784634-1784653 None:intergenic 60.0%
AGGAGCAGAGCAGCCCAGAA+AGG + chr8_3:1784641-1784660 None:intergenic 60.0%
CAATCCAGGCGTGATCCCAG+AGG + chr8_3:1784661-1784680 None:intergenic 60.0%
GTGAGGTCGGGGTGAGCTAC+TGG - chr8_3:1782821-1782840 Msa1268680:CDS 65.0%
CGGTGTGGCCGACCCAAAAG+TGG - chr8_3:1784236-1784255 Msa1268680:intron 65.0%
TCTGGGCTGCTCTGCTCCTC+TGG - chr8_3:1784642-1784661 Msa1268680:intron 65.0%
CTGGGCTGCTCTGCTCCTCT+GGG - chr8_3:1784643-1784662 Msa1268680:intron 65.0%
!! TGCTCCTCTGGGATCACGCC+TGG - chr8_3:1784654-1784673 Msa1268680:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 1781927 1786116 1781927 ID=Msa1268680;Name=Msa1268680
chr8_3 mRNA 1781927 1786116 1781927 ID=Msa1268680-mRNA-1;Parent=Msa1268680;Name=Msa1268680-mRNA-1;_AED=0.13;_eAED=0.13;_QI=195|0.33|0.25|0.75|0.33|0.25|4|0|224
chr8_3 exon 1781927 1782098 1781927 ID=Msa1268680-mRNA-1:exon:530;Parent=Msa1268680-mRNA-1
chr8_3 exon 1782782 1782977 1782782 ID=Msa1268680-mRNA-1:exon:529;Parent=Msa1268680-mRNA-1
chr8_3 exon 1785540 1785594 1785540 ID=Msa1268680-mRNA-1:exon:528;Parent=Msa1268680-mRNA-1
chr8_3 exon 1785673 1786116 1785673 ID=Msa1268680-mRNA-1:exon:527;Parent=Msa1268680-mRNA-1
chr8_3 five_prime_UTR 1785922 1786116 1785922 ID=Msa1268680-mRNA-1:five_prime_utr;Parent=Msa1268680-mRNA-1
chr8_3 CDS 1785673 1785921 1785673 ID=Msa1268680-mRNA-1:cds;Parent=Msa1268680-mRNA-1
chr8_3 CDS 1785540 1785594 1785540 ID=Msa1268680-mRNA-1:cds;Parent=Msa1268680-mRNA-1
chr8_3 CDS 1782782 1782977 1782782 ID=Msa1268680-mRNA-1:cds;Parent=Msa1268680-mRNA-1
chr8_3 CDS 1781927 1782098 1781927 ID=Msa1268680-mRNA-1:cds;Parent=Msa1268680-mRNA-1
Gene Sequence

>Msa1268680

ATGTCAACTTTTCACGTCGGCGGTAAGGTCGTAGACAAAGTTGATCTACTCCGGAAGAAGCAGTTGCCATGGCGCTTAGACGTTTTTCCTTTCGCTATTTTATACGGCGCGTGGATTTCAGTCATTCTTCCTAGTCTCGATTTCGTCGATGCTTGTATTGTTTTAGGTGCACTCGCTTCTTTTCATATTCTCGTCTGTCTCTTCACTGCTTGGTCCGTTGATTTCAAATGCTTTGCGTATTACAGCAAGGTTAAGAATATTGATCAGGCTGATTCCTGCAAGATAACTCCGGCGAAATTTTGTGGTTCTTCATTAGCTGTGGATTTGGAAGAAATTTATTTTGATTTTAGAAAACAATGTTTCGTTTATTCGAAGGAGAAGGGGACCTTTTGCAAACTTTCTTACCCTACCAAGGAAACATTTGGATATTATGTCAAGAGTAGTGGTCATGGTTCCGAAGCCAAAGTCTTTGCTGCTACTGAGAAATGGGGGCGGAATGTGTGGGTAAAAAATCTCTGGTACAGACCTTTTGCCTGGAGATGTTGTCTCTATAGGTCGCTCTGTGGTCAGAATGGAGAGGAGAAATCTGTACCTGCAGACATGCTTATTTTGGCTGGAAGTGCAATTGTGAATGAAGCTATTCTCACAGGCGAGTCTACTCCTCAATGGAAG
Protein sequence

>Msa1268680

MSTFHVGGKVVDKVDLLRKKQLPWRLDVFPFAILYGAWISVILPSLDFVDACIVLGALASFHILVCLFTAWSVDFKCFAYYSKVKNIDQADSCKITPAKFCGSSLAVDLEEIYFDFRKQCFVYSKEKGTFCKLSYPTKETFGYYVKSSGHGSEAKVFAATEKWGRNVWVKNLWYRPFAWRCCLYRSLCGQNGEEKSVPADMLILAGSAIVNEAILTGESTPQWK