AlfalfaGEDB | Msa1292840

M. sativa cultivar zhongmu-4 / Msa1292840

KEGG: -
GO: -
PFAM: PF14365.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292840	XP_013446150.2	96.205	448	17	0	1	448	1	448	0.0	914

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292840	A0A396GKI9	96.205	448	17	0	1	448	1	448	0.0	914

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0034210	Msa1292840	0.809720	1.645868e-50	-8.615850e-47
Msa0050660	Msa1292840	0.812142	4.919321e-51	-8.615850e-47
Msa0050700	Msa1292840	0.815302	9.911428e-52	-8.615850e-47
Msa0050790	Msa1292840	0.824953	6.105053e-54	-8.615850e-47
Msa0128310	Msa1292840	0.822748	2.006054e-53	-8.615850e-47
Msa0174440	Msa1292840	0.809634	1.717320e-50	-8.615850e-47
Msa0390970	Msa1292840	0.801441	8.991505e-49	-8.615850e-47
Msa0597270	Msa1292840	0.808619	2.833739e-50	-8.615850e-47
Msa1042160	Msa1292840	0.823604	1.266957e-53	-8.615850e-47
Msa1042190	Msa1292840	0.820736	5.859362e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292840	MtrunA17_Chr8g0368161	96.205	448	17	0	1	448	1	448	0.0	914
Msa1292840	MtrunA17_Chr5g0413131	69.710	449	114	7	20	448	17	463	0.0	631
Msa1292840	MtrunA17_Chr4g0058771	61.311	473	149	8	8	448	11	481	0.0	592
Msa1292840	MtrunA17_Chr3g0117271	69.251	387	116	3	63	448	112	496	0.0	576
Msa1292840	MtrunA17_Chr8g0368241	64.251	414	145	1	38	448	1	414	0.0	559
Msa1292840	MtrunA17_Chr2g0314461	67.342	395	120	5	57	448	18	406	0.0	547
Msa1292840	MtrunA17_Chr2g0314451	64.706	408	131	6	48	448	4	405	0.0	533
Msa1292840	MtrunA17_Chr2g0314471	62.379	412	142	6	44	448	1	406	0.0	525
Msa1292840	MtrunA17_Chr7g0274951	54.118	425	169	6	49	447	15	439	9.68e-164	468
Msa1292840	MtrunA17_Chr1g0189961	51.972	431	173	6	51	448	22	451	5.71e-159	457

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292840	AT1G10750.1	73.560	382	100	1	67	448	87	467	0.0	613
Msa1292840	AT1G23340.2	68.238	403	126	2	47	448	8	409	0.0	588
Msa1292840	AT1G23340.1	68.238	403	126	2	47	448	8	409	0.0	588
Msa1292840	AT1G70550.1	70.712	379	110	1	70	448	88	465	0.0	582
Msa1292840	AT5G50150.1	69.634	382	113	3	69	448	40	420	0.0	569
Msa1292840	AT1G55360.1	55.959	386	151	5	81	448	38	422	1.39e-158	455
Msa1292840	AT3G13510.1	53.511	413	161	7	53	448	21	419	3.03e-158	454
Msa1292840	AT5G18460.1	56.250	384	142	5	86	447	50	429	3.70e-157	452
Msa1292840	AT5G56530.2	53.222	419	154	7	51	447	21	419	2.05e-153	442
Msa1292840	AT5G56530.1	53.222	419	154	7	51	447	21	419	2.05e-153	442

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Find 124 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTAGCATAAATGTGT+GGG	0.619969	8_3:-49443047	Msa1292840:CDS
AAAATGTCCACTACCCATTT+GGG	0.415939	8_3:+49439992	None:intergenic
AAAATTATAACTTTCAATTG+TGG	0.487536	8_3:-49444280	Msa1292840:CDS
AAAATTGAAACGTGTTAGAA+TGG	0.456346	8_3:-49444159	Msa1292840:CDS
AAACAAGGAGAAGAAAATGA+AGG	0.472952	8_3:+49444771	None:intergenic
AAATATGTTCAGAGAGAGAA+AGG	0.428067	8_3:-49445037	Msa1292840:five_prime_UTR
AAATGTGTGGGTCCCACAAG+TGG	0.566747	8_3:-49443035	Msa1292840:CDS
AACATAGGATGGTCAAAAGC+TGG	0.536748	8_3:+49444469	None:intergenic
AACTTGTGAAACTCTTCATT+TGG	0.403131	8_3:+49444692	None:intergenic
AAGAAGGAACTAATAATGGG+TGG	0.603081	8_3:-49439845	Msa1292840:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATATGTTCAGAGAGAGAA+AGG	-	chr8_3:49439735-49439754	Msa1292840:three_prime_UTR	30.0%
AATATGTTCAGAGAGAGAAA+GGG	-	chr8_3:49439736-49439755	Msa1292840:three_prime_UTR	30.0%
AATACACACAAACACAAACA+TGG	-	chr8_3:49439950-49439969	Msa1292840:CDS	30.0%
AAACAAGGAGAAGAAAATGA+AGG	+	chr8_3:49440004-49440023	None:intergenic	30.0%
GTTTATTCTTCAGAAACAAG+TGG	-	chr8_3:49440038-49440057	Msa1292840:CDS	30.0%
AACTTGTGAAACTCTTCATT+TGG	+	chr8_3:49440083-49440102	None:intergenic	30.0%
ATGAAGAGTTTCACAAGTTT+AGG	-	chr8_3:49440084-49440103	Msa1292840:CDS	30.0%
ATGGAAATTGGTAAAGCATA+TGG	-	chr8_3:49440817-49440836	Msa1292840:intron	30.0%
TGGCTTATATAAAATTACGG+TGG	-	chr8_3:49440902-49440921	Msa1292840:intron	30.0%
ACATACTTTAGTCCTTATGT+AGG	+	chr8_3:49441031-49441050	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	49439713	49445081	49439713	ID=Msa1292840;Name=Msa1292840
chr8_3	mRNA	49439713	49445081	49439713	ID=Msa1292840-mRNA-1;Parent=Msa1292840;Name=Msa1292840-mRNA-1;_AED=0.06;_eAED=0.06;_QI=145\|1\|1\|1\|1\|1\|7\|81\|448
chr8_3	exon	49439713	49440171	49439713	ID=Msa1292840-mRNA-1:exon:13737;Parent=Msa1292840-mRNA-1
chr8_3	exon	49441623	49441769	49441623	ID=Msa1292840-mRNA-1:exon:13736;Parent=Msa1292840-mRNA-1
chr8_3	exon	49441906	49441959	49441906	ID=Msa1292840-mRNA-1:exon:13735;Parent=Msa1292840-mRNA-1
chr8_3	exon	49442946	49443110	49442946	ID=Msa1292840-mRNA-1:exon:13734;Parent=Msa1292840-mRNA-1
chr8_3	exon	49444136	49444345	49444136	ID=Msa1292840-mRNA-1:exon:13733;Parent=Msa1292840-mRNA-1
chr8_3	exon	49444450	49444536	49444450	ID=Msa1292840-mRNA-1:exon:13732;Parent=Msa1292840-mRNA-1
chr8_3	exon	49444631	49445081	49444631	ID=Msa1292840-mRNA-1:exon:13731;Parent=Msa1292840-mRNA-1
chr8_3	five_prime_UTR	49444937	49445081	49444937	ID=Msa1292840-mRNA-1:five_prime_utr;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49444631	49444936	49444631	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49444450	49444536	49444450	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49444136	49444345	49444136	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49442946	49443110	49442946	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49441906	49441959	49441906	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49441623	49441769	49441623	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	CDS	49439794	49440171	49439794	ID=Msa1292840-mRNA-1:cds;Parent=Msa1292840-mRNA-1
chr8_3	three_prime_UTR	49439713	49439793	49439713	ID=Msa1292840-mRNA-1:three_prime_utr;Parent=Msa1292840-mRNA-1

Gene Sequence

>Msa1292840

ATGGGGTTAGATTTGTTCCAATGGACAAAACCAAAATATAAGAACACTCAAATTTGTTTTCTTGATGCAAGAAAACAACACAAATGTGGAGAAATACACACAAACACAAACATGGATTTCAGCTTCCCAGTCATCTCCCTCCTCCTTCATTTTCTTCTCCTTGTTTCTTTGGTTTGTCATGTTTATTCTTCAGAAACAAGTGGCCAAAATCAAACTTTTCTACCAAATGAAGAGTTTCACAAGTTTAGGAGGACCATAAAAATGCATCTTCGACGGATCAACAAGCCTGCTGTTAAGACAATTCAGAGTCCTGATGGTGATATCATAGATTGTGTTATGTCTCATAGACAACCAGCTTTTGACCATCCTATGTTGAGAGGACAAAAACCATTGGATCCTCCGGAGAGACCAAGAGAGCATAAGCAAATGGGCATTGAAAATTATAACTTTCAATTGTGGAGTTTCTCAGGTGAATCATGTCCAGAAGGAACGATTCCGATTAGAAGAATAAAAGAAGATGACATGTTAAGAGCTTGCTCTATTAACACATTTGGAAGAAAATTGAAACGTGTTAGAATGGACACCACCGGCGATGGACATGAGCATGCAGTTGGATATGTGAGTGGGAATGAGTACTATGGAGCAAAAGCTAGCATAAATGTGTGGGTCCCACAAGTGGAAAGTCCATATGAATTCAGCTTGTCTCAAATATGGATCATCTCTGGTTCATTTGGAGTAGATCTCAATACCATTGAAGCTGGTTGGCAGGTCAGCCCAGAGCTCTATGGGGATAGCTACCCTAGATTCTTCACTTATTGGACGACTGATGCATATCAAGGAACTGGGTGCTACAATTTACTTTGCTCTGGCTTTGTTCAAACTAGCAGTAATATTGCTATTGGAGCAGCAATTTCTCCAACTTCTACATATAATGGAGGCCAATTTGATATAAGCTTAATGATCTGGAAGGATCCAAAGCATGGGAATTGGTGGCTTGAATATGGATCTGGAAACTTGATTGGGTATTGGCCATCATCCCTATTCACACACTTAAAGGATAGTGCAACAATGGTTCAATTTGGTGGAGAAATAGTGAATACAATGTCAACAGGGTCTCACACTTCTACCCAAATGGGTAGTGGACATTTTGCTGAGGAGGGTTTTGCAAAAGCTGCATACTTTAGAAATATGCAAGTTGTGGATTCTGATAACAGCTTGATTCCCTTGCCAAATCTCAATGTTGTAGCTAACCGTCCAAATTGTTATAATATACAAGAAGGAACTAATAATGGGTGGGGAAATTACTTTTACTATGGTGGTCCTGGAAGGAATGTAAAATGTCCCTAA

Protein sequence

>Msa1292840

MGLDLFQWTKPKYKNTQICFLDARKQHKCGEIHTNTNMDFSFPVISLLLHFLLLVSLVCHVYSSETSGQNQTFLPNEEFHKFRRTIKMHLRRINKPAVKTIQSPDGDIIDCVMSHRQPAFDHPMLRGQKPLDPPERPREHKQMGIENYNFQLWSFSGESCPEGTIPIRRIKEDDMLRACSINTFGRKLKRVRMDTTGDGHEHAVGYVSGNEYYGAKASINVWVPQVESPYEFSLSQIWIISGSFGVDLNTIEAGWQVSPELYGDSYPRFFTYWTTDAYQGTGCYNLLCSGFVQTSSNIAIGAAISPTSTYNGGQFDISLMIWKDPKHGNWWLEYGSGNLIGYWPSSLFTHLKDSATMVQFGGEIVNTMSTGSHTSTQMGSGHFAEEGFAKAAYFRNMQVVDSDNSLIPLPNLNVVANRPNCYNIQEGTNNGWGNYFYYGGPGRNVKCP*