Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1295300 | XP_003629413.1 | 95.135 | 370 | 18 | 0 | 1 | 370 | 1 | 370 | 0.0 | 733 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1295300 | sp|Q9SPV4|SAMT_CLABR | 58.760 | 371 | 135 | 5 | 1 | 368 | 1 | 356 | 2.37e-157 | 449 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1295300 | G7LDX4 | 95.135 | 370 | 18 | 0 | 1 | 370 | 1 | 370 | 0.0 | 733 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001920 | Msa1295300 | 0.813814 | 2.115840e-51 | -8.615850e-47 |
| Msa0020320 | Msa1295300 | 0.824732 | 6.881646e-54 | -8.615850e-47 |
| Msa0116510 | Msa1295300 | 0.801694 | 7.977133e-49 | -8.615850e-47 |
| Msa0610780 | Msa1295300 | 0.813155 | 2.953501e-51 | -8.615850e-47 |
| Msa0610810 | Msa1295300 | 0.800534 | 1.377944e-48 | -8.615850e-47 |
| Msa0640220 | Msa1295300 | 0.804684 | 1.918990e-49 | -8.615850e-47 |
| Msa0651310 | Msa1295300 | 0.802203 | 6.272651e-49 | -8.615850e-47 |
| Msa0697490 | Msa1295300 | 0.825516 | 4.492213e-54 | -8.615850e-47 |
| Msa0803530 | Msa1295300 | 0.801955 | 7.052155e-49 | -8.615850e-47 |
| Msa0148430 | Msa1295300 | 0.800256 | 1.569294e-48 | -8.615850e-47 |
| Msa0218430 | Msa1295300 | 0.829326 | 5.481720e-55 | -8.615850e-47 |
| Msa1295290 | Msa1295300 | 0.978482 | 6.451048e-146 | -8.615850e-47 |
| Msa1295300 | Msa1317240 | 0.801118 | 1.047086e-48 | -8.615850e-47 |
| Msa1295300 | Msa1460530 | 0.822342 | 2.493635e-53 | -8.615850e-47 |
| Msa0239610 | Msa1295300 | 0.810439 | 1.151937e-50 | -8.615850e-47 |
| Msa1136120 | Msa1295300 | 0.816449 | 5.499601e-52 | -8.615850e-47 |
| Msa1202680 | Msa1295300 | 0.820519 | 6.571431e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1295300 | MtrunA17_Chr8g0372821 | 95.135 | 370 | 18 | 0 | 1 | 370 | 1 | 370 | 0.0 | 733 |
| Msa1295300 | MtrunA17_Chr5g0404631 | 71.545 | 369 | 104 | 1 | 1 | 368 | 11 | 379 | 0.0 | 570 |
| Msa1295300 | MtrunA17_Chr3g0107591 | 71.351 | 370 | 105 | 1 | 1 | 370 | 1 | 369 | 0.0 | 561 |
| Msa1295300 | MtrunA17_Chr4g0008111 | 71.467 | 368 | 94 | 2 | 1 | 368 | 1 | 357 | 0.0 | 543 |
| Msa1295300 | MtrunA17_Chr8g0372671 | 68.207 | 368 | 109 | 1 | 1 | 368 | 1 | 360 | 0.0 | 527 |
| Msa1295300 | MtrunA17_Chr6g0475241 | 62.803 | 371 | 123 | 3 | 1 | 370 | 1 | 357 | 1.65e-167 | 472 |
| Msa1295300 | MtrunA17_Chr8g0343971 | 60.163 | 369 | 135 | 2 | 1 | 368 | 1 | 358 | 3.09e-161 | 456 |
| Msa1295300 | MtrunA17_Chr6g0475221 | 62.162 | 370 | 124 | 4 | 1 | 370 | 1 | 354 | 4.43e-160 | 452 |
| Msa1295300 | MtrunA17_Chr8g0343981 | 59.350 | 369 | 135 | 2 | 1 | 368 | 1 | 355 | 1.32e-159 | 452 |
| Msa1295300 | MtrunA17_Chr3g0144871 | 44.180 | 378 | 190 | 4 | 1 | 368 | 1 | 367 | 1.39e-113 | 335 |
| Msa1295300 | MtrunA17_Chr5g0419851 | 39.840 | 374 | 205 | 3 | 1 | 368 | 61 | 420 | 2.70e-99 | 300 |
| Msa1295300 | MtrunA17_Chr3g0107871 | 42.450 | 351 | 185 | 5 | 24 | 368 | 1 | 340 | 6.95e-94 | 284 |
| Msa1295300 | MtrunA17_Chr7g0252361 | 37.234 | 376 | 217 | 5 | 3 | 370 | 1 | 365 | 4.81e-85 | 262 |
| Msa1295300 | MtrunA17_Chr8g0372801 | 72.180 | 133 | 37 | 0 | 90 | 222 | 31 | 163 | 3.69e-68 | 212 |
| Msa1295300 | MtrunA17_Chr1g0155451 | 43.781 | 201 | 106 | 1 | 168 | 368 | 22 | 215 | 1.32e-55 | 181 |
| Msa1295300 | MtrunA17_Chr3g0100101 | 35.890 | 365 | 198 | 12 | 1 | 346 | 10 | 357 | 1.82e-54 | 184 |
| Msa1295300 | MtrunA17_Chr5g0444921 | 32.737 | 391 | 225 | 11 | 1 | 367 | 13 | 389 | 1.26e-51 | 176 |
| Msa1295300 | MtrunA17_Chr1g0208641 | 33.333 | 357 | 190 | 12 | 29 | 368 | 40 | 365 | 2.34e-51 | 175 |
| Msa1295300 | MtrunA17_Chr7g0252501 | 39.024 | 205 | 111 | 4 | 168 | 370 | 38 | 230 | 2.54e-44 | 152 |
| Msa1295300 | MtrunA17_Chr1g0155491 | 46.541 | 159 | 75 | 4 | 1 | 149 | 1 | 159 | 1.19e-41 | 144 |
| Msa1295300 | MtrunA17_Chr1g0155461 | 45.283 | 159 | 77 | 4 | 1 | 149 | 1 | 159 | 1.52e-40 | 141 |
| Msa1295300 | MtrunA17_Chr6g0455071 | 47.581 | 124 | 54 | 3 | 137 | 254 | 11 | 129 | 6.16e-34 | 122 |
| Msa1295300 | MtrunA17_Chr7g0252341 | 42.748 | 131 | 69 | 3 | 34 | 161 | 5 | 132 | 4.85e-28 | 107 |
| Msa1295300 | MtrunA17_Chr3g0099281 | 36.686 | 169 | 99 | 3 | 1 | 161 | 13 | 181 | 3.29e-27 | 106 |
| Msa1295300 | MtrunA17_Chr1g0155471 | 36.290 | 124 | 71 | 2 | 245 | 368 | 8 | 123 | 4.77e-23 | 93.2 |
| Msa1295300 | MtrunA17_Chr1g0155481 | 52.564 | 78 | 37 | 0 | 168 | 245 | 22 | 99 | 2.15e-22 | 92.0 |
| Msa1295300 | MtrunA17_Chr6g0455081 | 30.137 | 146 | 81 | 3 | 228 | 369 | 2 | 130 | 7.00e-17 | 76.6 |
| Msa1295300 | MtrunA17_Chr3g0099271 | 30.198 | 202 | 117 | 6 | 161 | 351 | 2 | 190 | 1.15e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1295300 | AT1G19640.1 | 44.730 | 389 | 192 | 7 | 1 | 368 | 1 | 387 | 1.10e-99 | 301 |
| Msa1295300 | AT3G11480.1 | 41.892 | 370 | 191 | 6 | 5 | 368 | 27 | 378 | 6.07e-98 | 296 |
| Msa1295300 | AT5G66430.1 | 41.160 | 362 | 202 | 5 | 9 | 368 | 1 | 353 | 4.42e-96 | 290 |
| Msa1295300 | AT4G36470.1 | 41.645 | 377 | 190 | 7 | 7 | 369 | 10 | 370 | 2.86e-95 | 289 |
| Msa1295300 | AT5G04370.1 | 42.857 | 364 | 193 | 5 | 7 | 368 | 39 | 389 | 4.40e-94 | 286 |
| Msa1295300 | AT5G04370.2 | 42.857 | 364 | 193 | 5 | 7 | 368 | 58 | 408 | 9.69e-94 | 286 |
| Msa1295300 | AT5G04370.3 | 42.857 | 364 | 193 | 5 | 7 | 368 | 44 | 394 | 9.90e-94 | 286 |
| Msa1295300 | AT5G38020.1 | 42.049 | 371 | 201 | 6 | 6 | 368 | 3 | 367 | 2.87e-91 | 278 |
| Msa1295300 | AT3G21950.1 | 41.333 | 375 | 198 | 8 | 6 | 368 | 3 | 367 | 9.79e-90 | 275 |
| Msa1295300 | AT5G04380.3 | 39.946 | 368 | 202 | 6 | 6 | 368 | 35 | 388 | 7.32e-89 | 273 |
| Msa1295300 | AT5G04380.1 | 39.402 | 368 | 197 | 7 | 6 | 368 | 31 | 377 | 1.42e-84 | 261 |
| Msa1295300 | AT3G21950.2 | 38.329 | 407 | 197 | 9 | 6 | 368 | 3 | 399 | 8.07e-84 | 260 |
| Msa1295300 | AT2G14060.1 | 37.265 | 373 | 212 | 5 | 6 | 368 | 11 | 371 | 4.27e-83 | 258 |
| Msa1295300 | AT5G04380.2 | 40.062 | 322 | 181 | 4 | 48 | 368 | 7 | 317 | 2.85e-82 | 254 |
| Msa1295300 | AT5G38020.2 | 42.073 | 328 | 177 | 5 | 48 | 368 | 7 | 328 | 4.93e-82 | 253 |
| Msa1295300 | AT2G14060.2 | 43.421 | 228 | 125 | 2 | 6 | 232 | 11 | 235 | 1.74e-64 | 206 |
| Msa1295300 | AT1G68040.1 | 34.865 | 370 | 202 | 8 | 3 | 359 | 7 | 350 | 2.33e-64 | 209 |
| Msa1295300 | AT5G55250.1 | 34.417 | 369 | 197 | 12 | 1 | 345 | 13 | 360 | 3.18e-57 | 191 |
| Msa1295300 | AT1G15125.1 | 31.467 | 375 | 214 | 11 | 9 | 368 | 1 | 347 | 4.35e-50 | 172 |
| Msa1295300 | AT5G37990.1 | 34.109 | 387 | 201 | 14 | 5 | 368 | 6 | 361 | 5.81e-50 | 172 |
| Msa1295300 | AT5G55250.2 | 33.436 | 326 | 176 | 9 | 41 | 345 | 17 | 322 | 3.35e-49 | 169 |
| Msa1295300 | AT5G38100.1 | 33.518 | 361 | 195 | 9 | 4 | 348 | 5 | 336 | 4.61e-49 | 169 |
| Msa1295300 | AT5G38780.1 | 33.058 | 363 | 196 | 10 | 4 | 348 | 5 | 338 | 5.16e-49 | 169 |
| Msa1295300 | AT5G37970.1 | 31.948 | 385 | 212 | 10 | 5 | 368 | 6 | 361 | 5.37e-48 | 166 |
| Msa1295300 | AT4G26420.1 | 34.435 | 363 | 198 | 13 | 3 | 344 | 7 | 350 | 4.00e-47 | 164 |
| Msa1295300 | AT5G38100.3 | 35.370 | 311 | 159 | 8 | 51 | 348 | 32 | 313 | 9.05e-47 | 162 |
| Msa1295300 | AT5G38780.2 | 34.185 | 313 | 163 | 9 | 50 | 348 | 6 | 289 | 2.09e-46 | 161 |
| Msa1295300 | AT5G56300.1 | 30.748 | 361 | 215 | 10 | 3 | 345 | 18 | 361 | 3.88e-46 | 162 |
| Msa1295300 | AT3G44840.1 | 32.410 | 361 | 197 | 10 | 3 | 348 | 1 | 329 | 2.13e-44 | 157 |
| Msa1295300 | AT3G44860.1 | 36.364 | 286 | 163 | 6 | 3 | 281 | 1 | 274 | 8.59e-41 | 147 |
| Msa1295300 | AT3G44870.1 | 36.014 | 286 | 161 | 7 | 3 | 281 | 1 | 271 | 4.08e-40 | 146 |
| Msa1295300 | AT1G66720.1 | 33.000 | 300 | 172 | 10 | 9 | 294 | 9 | 293 | 6.28e-39 | 142 |
| Msa1295300 | AT4G26420.3 | 38.095 | 231 | 119 | 8 | 3 | 220 | 7 | 226 | 3.59e-38 | 137 |
| Msa1295300 | AT1G66700.1 | 32.082 | 293 | 157 | 7 | 9 | 281 | 9 | 279 | 1.49e-37 | 139 |
| Msa1295300 | AT4G26420.2 | 38.739 | 222 | 118 | 7 | 9 | 220 | 1 | 214 | 1.86e-36 | 133 |
| Msa1295300 | AT5G38100.2 | 38.117 | 223 | 114 | 6 | 4 | 213 | 5 | 216 | 5.73e-35 | 129 |
| Msa1295300 | AT5G38100.4 | 38.117 | 223 | 114 | 6 | 4 | 213 | 5 | 216 | 6.35e-35 | 129 |
| Msa1295300 | AT1G15125.2 | 35.160 | 219 | 119 | 6 | 9 | 217 | 1 | 206 | 1.98e-33 | 125 |
| Msa1295300 | AT1G66690.1 | 32.107 | 299 | 165 | 7 | 1 | 281 | 1 | 279 | 7.73e-33 | 126 |
| Msa1295300 | AT1G66700.3 | 31.907 | 257 | 133 | 7 | 9 | 245 | 9 | 243 | 5.64e-30 | 116 |
Find 81 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAAATATATTGGGGAAAA+TGG | 0.160397 | 8_3:-53444409 | None:intergenic |
| CCACAACTTTCTAAATATAT+TGG | 0.193245 | 8_3:-53444418 | None:intergenic |
| TCTGCAATTCCTAAGCTATT+TGG | 0.193298 | 8_3:-53442602 | None:intergenic |
| CACAACTTTCTAAATATATT+GGG | 0.228990 | 8_3:-53444417 | None:intergenic |
| GCAGACTTGGGTTGCTCTTC+CGG | 0.254531 | 8_3:+53442620 | Msa1295300:CDS |
| CAGCCTTATGTGGCTATCTA+AGG | 0.259712 | 8_3:+53442916 | Msa1295300:CDS |
| GGAGTTCCTGGTTCTTTCTA+TGG | 0.263034 | 8_3:+53442848 | Msa1295300:CDS |
| GTTCAAGAAAACCTTGTATT+CGG | 0.272656 | 8_3:-53442720 | None:intergenic |
| GCAAGGAGTGTTGCTACATT+TGG | 0.284975 | 8_3:+53443515 | Msa1295300:CDS |
| TTGCTACATTTGGGAACTTA+TGG | 0.286140 | 8_3:+53443525 | Msa1295300:CDS |
| GATTATGGCTTCTTCTCTAA+TGG | 0.308035 | 8_3:-53442558 | None:intergenic |
| CTGCAATTCCTAAGCTATTT+GGG | 0.311964 | 8_3:-53442601 | None:intergenic |
| TGCTATATATCTGGAGTTCC+TGG | 0.312996 | 8_3:+53442836 | Msa1295300:CDS |
| ATTACAGTAGAGACTGATTA+TGG | 0.317438 | 8_3:-53442573 | None:intergenic |
| TCTTAATGATATGGTCTTGC+AGG | 0.323770 | 8_3:+53443555 | Msa1295300:CDS |
| GGAGAATGTGAACAACAATA+AGG | 0.339279 | 8_3:+53443327 | Msa1295300:CDS |
| GGCTATAGCTCTTAATGATA+TGG | 0.347430 | 8_3:+53443546 | Msa1295300:CDS |
| ACAAAATGCAAACTTTGATT+AGG | 0.356644 | 8_3:-53442881 | None:intergenic |
| ATGGATCAGTGCTATATATC+TGG | 0.358943 | 8_3:+53442827 | Msa1295300:CDS |
| GTGTCCATCGGAAGTGAAAT+TGG | 0.373571 | 8_3:+53444061 | Msa1295300:CDS |
| TTCCGGACCAAACACTTTGT+TGG | 0.378768 | 8_3:+53442637 | Msa1295300:CDS |
| CAAGGAGTGTTGCTACATTT+GGG | 0.384196 | 8_3:+53443516 | Msa1295300:CDS |
| TTCAAGAAAACCTTGTATTC+GGG | 0.384731 | 8_3:-53442719 | None:intergenic |
| CATAGCTAAGAACTAATATC+TGG | 0.397399 | 8_3:-53444654 | None:intergenic |
| ATCATTTACCATCGATCGAT+TGG | 0.399987 | 8_3:+53444100 | Msa1295300:CDS |
| TGCAAACAACTCCTTAGTTC+AGG | 0.408183 | 8_3:+53442257 | Msa1295300:CDS |
| TTCCTGGTTCTTTCTATGGC+AGG | 0.412204 | 8_3:+53442852 | Msa1295300:CDS |
| TCATTTACCATCGATCGATT+GGG | 0.423277 | 8_3:+53444101 | Msa1295300:CDS |
| TTATTGTTGTTCACATTCTC+CGG | 0.423470 | 8_3:-53443325 | None:intergenic |
| ATCAGAATTTATCAAGGTTG+TGG | 0.432422 | 8_3:+53442664 | Msa1295300:CDS |
| AGGATTTCTAGTCAATAATA+CGG | 0.440066 | 8_3:-53444360 | None:intergenic |
| GATACCACCAACAAAGTGTT+TGG | 0.447192 | 8_3:-53442644 | None:intergenic |
| CGGTGAAACAGAAACTGAAA+TGG | 0.453352 | 8_3:+53442808 | Msa1295300:CDS |
| CCTTTGCTAATTAGCCACTT+TGG | 0.456393 | 8_3:+53444248 | Msa1295300:CDS |
| TATGCATTAAGTACATTTGA+GGG | 0.463714 | 8_3:-53443376 | None:intergenic |
| AGAAAGTTGTGGTGTGTTGT+TGG | 0.472283 | 8_3:+53444429 | Msa1295300:three_prime_UTR |
| ATAAATTTATTATACAACTT+AGG | 0.481986 | 8_3:-53444562 | None:intergenic |
| GAGAATGTGAACAACAATAA+GGG | 0.486332 | 8_3:+53443328 | Msa1295300:CDS |
| CGGACCAAACACTTTGTTGG+TGG | 0.489740 | 8_3:+53442640 | Msa1295300:CDS |
| ATTGGAATGCTCTCGATCAA+TGG | 0.490851 | 8_3:+53444138 | Msa1295300:CDS |
| TAGCTTAGGAATTGCAGACT+TGG | 0.496344 | 8_3:+53442607 | Msa1295300:CDS |
| GTATGCATTAAGTACATTTG+AGG | 0.501880 | 8_3:-53443377 | None:intergenic |
| AGCTTAGGAATTGCAGACTT+GGG | 0.505553 | 8_3:+53442608 | Msa1295300:CDS |
| GGTGGTATCAGAATTTATCA+AGG | 0.507106 | 8_3:+53442658 | Msa1295300:CDS |
| TAGCCACATAAGGCTGTAAG+AGG | 0.511113 | 8_3:-53442909 | None:intergenic |
| AATACATACCCAAATAGCTT+AGG | 0.511941 | 8_3:+53442593 | Msa1295300:CDS |
| TCGTGAATCTCCCGAATACA+AGG | 0.518497 | 8_3:+53442709 | Msa1295300:CDS |
| ATTCCTCTTACAGCCTTATG+TGG | 0.518669 | 8_3:+53442906 | Msa1295300:CDS |
| CCAATATATTTAGAAAGTTG+TGG | 0.520390 | 8_3:+53444418 | Msa1295300:three_prime_UTR |
| ATCAAGAAATCTTAGTTGAT+CGG | 0.522974 | 8_3:+53444303 | Msa1295300:CDS |
| ATGGACACGGAGTGTATTGA+GGG | 0.523168 | 8_3:-53444046 | None:intergenic |
| CATTTACCATCGATCGATTG+GGG | 0.529876 | 8_3:+53444102 | Msa1295300:CDS |
| CGTGCAGAGGAAGTAGTTGA+AGG | 0.533351 | 8_3:+53443442 | Msa1295300:CDS |
| AAACCTTAGATAGCCACATA+AGG | 0.534886 | 8_3:-53442919 | None:intergenic |
| ACTTCCAATTTCACTTCCGA+TGG | 0.537214 | 8_3:-53444065 | None:intergenic |
| TCACAAGTACTGCACATGAA+TGG | 0.538311 | 8_3:+53442213 | Msa1295300:CDS |
| CACCAACAAAGTGTTTGGTC+CGG | 0.545185 | 8_3:-53442639 | None:intergenic |
| GATGGACACGGAGTGTATTG+AGG | 0.546758 | 8_3:-53444047 | None:intergenic |
| ATTCATGTATACCTGAACTA+AGG | 0.548252 | 8_3:-53442268 | None:intergenic |
| CAATAAGGGAAACATTTACA+TGG | 0.550411 | 8_3:+53443342 | Msa1295300:CDS |
| ACAACTTTCTAAATATATTG+GGG | 0.568794 | 8_3:-53444416 | None:intergenic |
| AAATTGGAAGTTCTCAAAGA+AGG | 0.571795 | 8_3:+53444077 | Msa1295300:CDS |
| ACATAAGGCTGTAAGAGGAA+TGG | 0.572052 | 8_3:-53442904 | None:intergenic |
| CTGCACATGAATGGAGGTGT+TGG | 0.572205 | 8_3:+53442222 | Msa1295300:CDS |
| GTGCAGAGGAAGTAGTTGAA+GGG | 0.581334 | 8_3:+53443443 | Msa1295300:CDS |
| ATGTCTGAATCACTCGGCGA+TGG | 0.582272 | 8_3:+53444185 | Msa1295300:CDS |
| AATTAGCAAAGGTTCAGAAA+CGG | 0.583320 | 8_3:-53444237 | None:intergenic |
| AATTTCACTTCCGATGGACA+CGG | 0.587997 | 8_3:-53444059 | None:intergenic |
| TCAATACACTCCGTGTCCAT+CGG | 0.588900 | 8_3:+53444049 | Msa1295300:CDS |
| CCAAAGTGGCTAATTAGCAA+AGG | 0.591173 | 8_3:-53444248 | None:intergenic |
| CTTAGTTGATCGGATGTCCA+AGG | 0.603686 | 8_3:+53444313 | Msa1295300:CDS |
| ATCCTGCCATAGAAAGAACC+AGG | 0.607110 | 8_3:-53442854 | None:intergenic |
| ATATTATTGAAGTCGTTACC+CGG | 0.611901 | 8_3:-53442749 | None:intergenic |
| TGCAGAGGAAGTAGTTGAAG+GGG | 0.612981 | 8_3:+53443444 | Msa1295300:CDS |
| CAATGATAGACTCTCCAAAG+TGG | 0.620746 | 8_3:-53444262 | None:intergenic |
| TCTCAAATGTCTGAATCACT+CGG | 0.620788 | 8_3:+53444179 | Msa1295300:CDS |
| AAGTGACAAGATGTATAGCA+AGG | 0.623819 | 8_3:+53443498 | Msa1295300:CDS |
| ATGCATTAAGTACATTTGAG+GGG | 0.624171 | 8_3:-53443375 | None:intergenic |
| ATTTACCATCGATCGATTGG+GGG | 0.660045 | 8_3:+53444103 | Msa1295300:CDS |
| CAAGTACTGCACATGAATGG+AGG | 0.661451 | 8_3:+53442216 | Msa1295300:CDS |
| GCAGAGGAAGTAGTTGAAGG+GGG | 0.713278 | 8_3:+53443445 | Msa1295300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCATATAATATTTTTTTTTA+AGG | + | chr8_3:53442458-53442477 | Msa1295300:intron | 10.0% |
| !! | AAATCCTTAAAAAATTATTA+GGG | + | chr8_3:53444376-53444395 | Msa1295300:exon | 10.0% |
| !! | ATAAATTTATTATACAACTT+AGG | - | chr8_3:53444565-53444584 | None:intergenic | 10.0% |
| !!! | ATAATAAATTTATTTTTACG+TGG | + | chr8_3:53444572-53444591 | Msa1295300:three_prime_UTR | 10.0% |
| !!! | TAATAAATTTATTTTTACGT+GGG | + | chr8_3:53444573-53444592 | Msa1295300:three_prime_UTR | 10.0% |
| !! | ATTATATATGAACACATAAT+AGG | - | chr8_3:53442341-53442360 | None:intergenic | 15.0% |
| !! | GAAATTACATATTAATATCT+TGG | - | chr8_3:53443971-53443990 | None:intergenic | 15.0% |
| !! | GAAATCCTTAAAAAATTATT+AGG | + | chr8_3:53444375-53444394 | Msa1295300:exon | 15.0% |
| !!! | TAAACCCTAATAATTTTTTA+AGG | - | chr8_3:53444383-53444402 | None:intergenic | 15.0% |
| !! | AATGCTATATATAACAGAAT+CGG | - | chr8_3:53442369-53442388 | None:intergenic | 20.0% |
| !!! | GTTTTTGTAATTGATGTAAT+TGG | + | chr8_3:53442498-53442517 | Msa1295300:intron | 20.0% |
| !! | GTAAATGTATATGTTGTTAT+TGG | + | chr8_3:53443655-53443674 | Msa1295300:intron | 20.0% |
| !! | TTAAAATATGCACTATCTTT+AGG | + | chr8_3:53443834-53443853 | Msa1295300:intron | 20.0% |
| !! | TAAAATATGCACTATCTTTA+GGG | + | chr8_3:53443835-53443854 | Msa1295300:intron | 20.0% |
| !! | TTAATTATATTTAGGGACAA+AGG | + | chr8_3:53443895-53443914 | Msa1295300:intron | 20.0% |
| !!! | TATGTAATTTCTTTTCTATG+TGG | + | chr8_3:53443980-53443999 | Msa1295300:intron | 20.0% |
| !! | ACAACTTTCTAAATATATTG+GGG | - | chr8_3:53444419-53444438 | None:intergenic | 20.0% |
| !! | CACAACTTTCTAAATATATT+GGG | - | chr8_3:53444420-53444439 | None:intergenic | 20.0% |
| !! | TGTGATTTAAAAAAAACACA+TGG | + | chr8_3:53444486-53444505 | Msa1295300:three_prime_UTR | 20.0% |
| ! | ATGCTATATATAACAGAATC+GGG | - | chr8_3:53442368-53442387 | None:intergenic | 25.0% |
| !! | CTTTATTATTTTCAGCTAGA+AGG | - | chr8_3:53442428-53442447 | None:intergenic | 25.0% |
| ! | ACAAAATGCAAACTTTGATT+AGG | - | chr8_3:53442884-53442903 | None:intergenic | 25.0% |
| !!! | TATTTTTGTGTCTTAGTCAA+TGG | + | chr8_3:53442971-53442990 | Msa1295300:intron | 25.0% |
| ! | TAAGATGAAAAATCAACTGA+GGG | - | chr8_3:53443146-53443165 | None:intergenic | 25.0% |
| ! | ATAAGATGAAAAATCAACTG+AGG | - | chr8_3:53443147-53443166 | None:intergenic | 25.0% |
| ! | TATGCATTAAGTACATTTGA+GGG | - | chr8_3:53443379-53443398 | None:intergenic | 25.0% |
| !!! | GAGAAAAATCTCTTTGAAAT+TGG | - | chr8_3:53443408-53443427 | None:intergenic | 25.0% |
| ! | TCCTAAATATAATGTGTTAG+TGG | + | chr8_3:53443590-53443609 | Msa1295300:intron | 25.0% |
| ! | ACCACTAACACATTATATTT+AGG | - | chr8_3:53443594-53443613 | None:intergenic | 25.0% |
| !! | ATTTCTTTTCTATGTGGATT+AGG | + | chr8_3:53443986-53444005 | Msa1295300:intron | 25.0% |
| !!! | TTTCTTTTCTATGTGGATTA+GGG | + | chr8_3:53443987-53444006 | Msa1295300:intron | 25.0% |
| ! | ATCAAGAAATCTTAGTTGAT+CGG | + | chr8_3:53444303-53444322 | Msa1295300:CDS | 25.0% |
| !!! | AGGATTTCTAGTCAATAATA+CGG | - | chr8_3:53444363-53444382 | None:intergenic | 25.0% |
| ! | TCTAAATATATTGGGGAAAA+TGG | - | chr8_3:53444412-53444431 | None:intergenic | 25.0% |
| ! | CCACAACTTTCTAAATATAT+TGG | - | chr8_3:53444421-53444440 | None:intergenic | 25.0% |
| ! | CCAATATATTTAGAAAGTTG+TGG | + | chr8_3:53444418-53444437 | Msa1295300:three_prime_UTR | 25.0% |
| !! | TTTCATATCGCCAAATTTTT+AGG | + | chr8_3:53444622-53444641 | Msa1295300:three_prime_UTR | 25.0% |
| !! | TTCATATCGCCAAATTTTTA+GGG | + | chr8_3:53444623-53444642 | Msa1295300:three_prime_UTR | 25.0% |
| ATTCATGTATACCTGAACTA+AGG | - | chr8_3:53442271-53442290 | None:intergenic | 30.0% | |
| ATTACAGTAGAGACTGATTA+TGG | - | chr8_3:53442576-53442595 | None:intergenic | 30.0% | |
| AATACATACCCAAATAGCTT+AGG | + | chr8_3:53442593-53442612 | Msa1295300:CDS | 30.0% | |
| ATCAGAATTTATCAAGGTTG+TGG | + | chr8_3:53442664-53442683 | Msa1295300:CDS | 30.0% | |
| TTCAAGAAAACCTTGTATTC+GGG | - | chr8_3:53442722-53442741 | None:intergenic | 30.0% | |
| GTTCAAGAAAACCTTGTATT+CGG | - | chr8_3:53442723-53442742 | None:intergenic | 30.0% | |
| ATATTATTGAAGTCGTTACC+CGG | - | chr8_3:53442752-53442771 | None:intergenic | 30.0% | |
| AATCCTTAACATGTGTCATA+GGG | + | chr8_3:53443113-53443132 | Msa1295300:intron | 30.0% | |
| !! | TTGTTGGTTGGTGATTATTT+AGG | + | chr8_3:53443300-53443319 | Msa1295300:intron | 30.0% |
| ! | TTATTGTTGTTCACATTCTC+CGG | - | chr8_3:53443328-53443347 | None:intergenic | 30.0% |
| GAGAATGTGAACAACAATAA+GGG | + | chr8_3:53443328-53443347 | Msa1295300:CDS | 30.0% | |
| CAATAAGGGAAACATTTACA+TGG | + | chr8_3:53443342-53443361 | Msa1295300:CDS | 30.0% | |
| ATGCATTAAGTACATTTGAG+GGG | - | chr8_3:53443378-53443397 | None:intergenic | 30.0% | |
| GTATGCATTAAGTACATTTG+AGG | - | chr8_3:53443380-53443399 | None:intergenic | 30.0% | |
| ! | CGCATGATTCTAACATTTTT+AGG | + | chr8_3:53443469-53443488 | Msa1295300:CDS | 30.0% |
| ! | ATCAAGCTTCTCTTCTTTTA+TGG | - | chr8_3:53444015-53444034 | None:intergenic | 30.0% |
| AAATTGGAAGTTCTCAAAGA+AGG | + | chr8_3:53444077-53444096 | Msa1295300:CDS | 30.0% | |
| AATTAGCAAAGGTTCAGAAA+CGG | - | chr8_3:53444240-53444259 | None:intergenic | 30.0% | |
| ! | TGGCGATATGAAATTTTACA+TGG | - | chr8_3:53444615-53444634 | None:intergenic | 30.0% |
| !! | TCATATCGCCAAATTTTTAG+GGG | + | chr8_3:53444624-53444643 | Msa1295300:three_prime_UTR | 30.0% |
| CATAGCTAAGAACTAATATC+TGG | - | chr8_3:53444657-53444676 | None:intergenic | 30.0% | |
| GATTATGGCTTCTTCTCTAA+TGG | - | chr8_3:53442561-53442580 | None:intergenic | 35.0% | |
| CTGCAATTCCTAAGCTATTT+GGG | - | chr8_3:53442604-53442623 | None:intergenic | 35.0% | |
| TCTGCAATTCCTAAGCTATT+TGG | - | chr8_3:53442605-53442624 | None:intergenic | 35.0% | |
| GGTGGTATCAGAATTTATCA+AGG | + | chr8_3:53442658-53442677 | Msa1295300:CDS | 35.0% | |
| ATGGATCAGTGCTATATATC+TGG | + | chr8_3:53442827-53442846 | Msa1295300:CDS | 35.0% | |
| AAACCTTAGATAGCCACATA+AGG | - | chr8_3:53442922-53442941 | None:intergenic | 35.0% | |
| GAATCCTTAACATGTGTCAT+AGG | + | chr8_3:53443112-53443131 | Msa1295300:intron | 35.0% | |
| !! | ATGGTGGAGAACATTTTTGT+TGG | + | chr8_3:53443284-53443303 | Msa1295300:intron | 35.0% |
| ! | TGGAGAACATTTTTGTTGGT+TGG | + | chr8_3:53443288-53443307 | Msa1295300:intron | 35.0% |
| !! | GTTGGTGATTATTTAGGTTC+CGG | + | chr8_3:53443306-53443325 | Msa1295300:intron | 35.0% |
| GGAGAATGTGAACAACAATA+AGG | + | chr8_3:53443327-53443346 | Msa1295300:CDS | 35.0% | |
| AAGTGACAAGATGTATAGCA+AGG | + | chr8_3:53443498-53443517 | Msa1295300:CDS | 35.0% | |
| TTGCTACATTTGGGAACTTA+TGG | + | chr8_3:53443525-53443544 | Msa1295300:CDS | 35.0% | |
| GGCTATAGCTCTTAATGATA+TGG | + | chr8_3:53443546-53443565 | Msa1295300:CDS | 35.0% | |
| ! | TCTTAATGATATGGTCTTGC+AGG | + | chr8_3:53443555-53443574 | Msa1295300:CDS | 35.0% |
| ATCATTTACCATCGATCGAT+TGG | + | chr8_3:53444100-53444119 | Msa1295300:CDS | 35.0% | |
| TCATTTACCATCGATCGATT+GGG | + | chr8_3:53444101-53444120 | Msa1295300:CDS | 35.0% | |
| TCTCAAATGTCTGAATCACT+CGG | + | chr8_3:53444179-53444198 | Msa1295300:CDS | 35.0% | |
| !! | GACAAACTTAGTTTTCTCCT+TGG | - | chr8_3:53444333-53444352 | None:intergenic | 35.0% |
| GCGATATTCCCCTAAAAATT+TGG | - | chr8_3:53444635-53444654 | None:intergenic | 35.0% | |
| ! | TCACAAGTACTGCACATGAA+TGG | + | chr8_3:53442213-53442232 | Msa1295300:CDS | 40.0% |
| TGCAAACAACTCCTTAGTTC+AGG | + | chr8_3:53442257-53442276 | Msa1295300:CDS | 40.0% | |
| TAGCTTAGGAATTGCAGACT+TGG | + | chr8_3:53442607-53442626 | Msa1295300:CDS | 40.0% | |
| AGCTTAGGAATTGCAGACTT+GGG | + | chr8_3:53442608-53442627 | Msa1295300:CDS | 40.0% | |
| ! | GATACCACCAACAAAGTGTT+TGG | - | chr8_3:53442647-53442666 | None:intergenic | 40.0% |
| ! | GGTTTTCTTGAACGATTTGC+CGG | + | chr8_3:53442730-53442749 | Msa1295300:CDS | 40.0% |
| ! | GTTTTCTTGAACGATTTGCC+GGG | + | chr8_3:53442731-53442750 | Msa1295300:CDS | 40.0% |
| AACTTCAAAAAGAGACTGCG+CGG | + | chr8_3:53442788-53442807 | Msa1295300:CDS | 40.0% | |
| CGGTGAAACAGAAACTGAAA+TGG | + | chr8_3:53442808-53442827 | Msa1295300:CDS | 40.0% | |
| ! | TGCTATATATCTGGAGTTCC+TGG | + | chr8_3:53442836-53442855 | Msa1295300:CDS | 40.0% |
| ACATAAGGCTGTAAGAGGAA+TGG | - | chr8_3:53442907-53442926 | None:intergenic | 40.0% | |
| ! | ATTCCTCTTACAGCCTTATG+TGG | + | chr8_3:53442906-53442925 | Msa1295300:CDS | 40.0% |
| GACGAATGAGTTGCTTAACA+TGG | + | chr8_3:53443265-53443284 | Msa1295300:intron | 40.0% | |
| GAATGAGTTGCTTAACATGG+TGG | + | chr8_3:53443268-53443287 | Msa1295300:intron | 40.0% | |
| !! | CAAGGAGTGTTGCTACATTT+GGG | + | chr8_3:53443516-53443535 | Msa1295300:CDS | 40.0% |
| AATTTCACTTCCGATGGACA+CGG | - | chr8_3:53444062-53444081 | None:intergenic | 40.0% | |
| ACTTCCAATTTCACTTCCGA+TGG | - | chr8_3:53444068-53444087 | None:intergenic | 40.0% | |
| CATTTACCATCGATCGATTG+GGG | + | chr8_3:53444102-53444121 | Msa1295300:CDS | 40.0% | |
| ATTTACCATCGATCGATTGG+GGG | + | chr8_3:53444103-53444122 | Msa1295300:CDS | 40.0% | |
| ATTGGAATGCTCTCGATCAA+TGG | + | chr8_3:53444138-53444157 | Msa1295300:CDS | 40.0% | |
| CCAAAGTGGCTAATTAGCAA+AGG | - | chr8_3:53444251-53444270 | None:intergenic | 40.0% | |
| CCTTTGCTAATTAGCCACTT+TGG | + | chr8_3:53444248-53444267 | Msa1295300:CDS | 40.0% | |
| CAATGATAGACTCTCCAAAG+TGG | - | chr8_3:53444265-53444284 | None:intergenic | 40.0% | |
| AGAAAGTTGTGGTGTGTTGT+TGG | + | chr8_3:53444429-53444448 | Msa1295300:three_prime_UTR | 40.0% | |
| ! | CAAGTACTGCACATGAATGG+AGG | + | chr8_3:53442216-53442235 | Msa1295300:CDS | 45.0% |
| TTCCGGACCAAACACTTTGT+TGG | + | chr8_3:53442637-53442656 | Msa1295300:CDS | 45.0% | |
| ! | CACCAACAAAGTGTTTGGTC+CGG | - | chr8_3:53442642-53442661 | None:intergenic | 45.0% |
| TCGTGAATCTCCCGAATACA+AGG | + | chr8_3:53442709-53442728 | Msa1295300:CDS | 45.0% | |
| ! | GGAGTTCCTGGTTCTTTCTA+TGG | + | chr8_3:53442848-53442867 | Msa1295300:CDS | 45.0% |
| ! | TTCCTGGTTCTTTCTATGGC+AGG | + | chr8_3:53442852-53442871 | Msa1295300:CDS | 45.0% |
| ATCCTGCCATAGAAAGAACC+AGG | - | chr8_3:53442857-53442876 | None:intergenic | 45.0% | |
| TAGCCACATAAGGCTGTAAG+AGG | - | chr8_3:53442912-53442931 | None:intergenic | 45.0% | |
| ! | CAGCCTTATGTGGCTATCTA+AGG | + | chr8_3:53442916-53442935 | Msa1295300:CDS | 45.0% |
| GTGCCCTATGACACATGTTA+AGG | - | chr8_3:53443119-53443138 | None:intergenic | 45.0% | |
| !! | TTTTCTCAAGTGTCGTGCAG+AGG | + | chr8_3:53443429-53443448 | Msa1295300:CDS | 45.0% |
| GTGCAGAGGAAGTAGTTGAA+GGG | + | chr8_3:53443443-53443462 | Msa1295300:CDS | 45.0% | |
| TGCAGAGGAAGTAGTTGAAG+GGG | + | chr8_3:53443444-53443463 | Msa1295300:CDS | 45.0% | |
| !! | GCAAGGAGTGTTGCTACATT+TGG | + | chr8_3:53443515-53443534 | Msa1295300:CDS | 45.0% |
| ATGGACACGGAGTGTATTGA+GGG | - | chr8_3:53444049-53444068 | None:intergenic | 45.0% | |
| TCAATACACTCCGTGTCCAT+CGG | + | chr8_3:53444049-53444068 | Msa1295300:CDS | 45.0% | |
| GTGTCCATCGGAAGTGAAAT+TGG | + | chr8_3:53444061-53444080 | Msa1295300:CDS | 45.0% | |
| !! | TGGGGGTTTCTGAAGTGAAT+TGG | + | chr8_3:53444120-53444139 | Msa1295300:CDS | 45.0% |
| CTTAGTTGATCGGATGTCCA+AGG | + | chr8_3:53444313-53444332 | Msa1295300:CDS | 45.0% | |
| !!! | CATATAATATTTTTTTTTAA+GGG | + | chr8_3:53442459-53442478 | Msa1295300:intron | 5.0% |
| !! | AAAAAAACTTAATTATATTT+AGG | + | chr8_3:53443887-53443906 | Msa1295300:intron | 5.0% |
| !! | AAAAAACTTAATTATATTTA+GGG | + | chr8_3:53443888-53443907 | Msa1295300:intron | 5.0% |
| ! | CTGCACATGAATGGAGGTGT+TGG | + | chr8_3:53442222-53442241 | Msa1295300:CDS | 50.0% |
| CGGACCAAACACTTTGTTGG+TGG | + | chr8_3:53442640-53442659 | Msa1295300:CDS | 50.0% | |
| CGTGCAGAGGAAGTAGTTGA+AGG | + | chr8_3:53443442-53443461 | Msa1295300:CDS | 50.0% | |
| GCAGAGGAAGTAGTTGAAGG+GGG | + | chr8_3:53443445-53443464 | Msa1295300:CDS | 50.0% | |
| GATGGACACGGAGTGTATTG+AGG | - | chr8_3:53444050-53444069 | None:intergenic | 50.0% | |
| AGAAACCCCCAATCGATCGA+TGG | - | chr8_3:53444111-53444130 | None:intergenic | 50.0% | |
| ATGTCTGAATCACTCGGCGA+TGG | + | chr8_3:53444185-53444204 | Msa1295300:CDS | 50.0% | |
| ! | GCAGACTTGGGTTGCTCTTC+CGG | + | chr8_3:53442620-53442639 | Msa1295300:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 53442145 | 53444690 | 53442145 | ID=Msa1295300;Name=Msa1295300 |
| chr8_3 | mRNA | 53442145 | 53444690 | 53442145 | ID=Msa1295300-mRNA-1;Parent=Msa1295300;Name=Msa1295300-mRNA-1;_AED=0.30;_eAED=0.30;_QI=59|1|1|1|1|1|4|305|370 |
| chr8_3 | exon | 53442145 | 53442278 | 53442145 | ID=Msa1295300-mRNA-1:exon:15327;Parent=Msa1295300-mRNA-1 |
| chr8_3 | exon | 53442533 | 53442937 | 53442533 | ID=Msa1295300-mRNA-1:exon:15328;Parent=Msa1295300-mRNA-1 |
| chr8_3 | exon | 53443322 | 53443576 | 53443322 | ID=Msa1295300-mRNA-1:exon:15329;Parent=Msa1295300-mRNA-1 |
| chr8_3 | exon | 53444008 | 53444690 | 53444008 | ID=Msa1295300-mRNA-1:exon:15330;Parent=Msa1295300-mRNA-1 |
| chr8_3 | five_prime_UTR | 53442145 | 53442203 | 53442145 | ID=Msa1295300-mRNA-1:five_prime_utr;Parent=Msa1295300-mRNA-1 |
| chr8_3 | CDS | 53442204 | 53442278 | 53442204 | ID=Msa1295300-mRNA-1:cds;Parent=Msa1295300-mRNA-1 |
| chr8_3 | CDS | 53442533 | 53442937 | 53442533 | ID=Msa1295300-mRNA-1:cds;Parent=Msa1295300-mRNA-1 |
| chr8_3 | CDS | 53443322 | 53443576 | 53443322 | ID=Msa1295300-mRNA-1:cds;Parent=Msa1295300-mRNA-1 |
| chr8_3 | CDS | 53444008 | 53444385 | 53444008 | ID=Msa1295300-mRNA-1:cds;Parent=Msa1295300-mRNA-1 |
| chr8_3 | three_prime_UTR | 53444386 | 53444690 | 53444386 | ID=Msa1295300-mRNA-1:three_prime_utr;Parent=Msa1295300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
