AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0177220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177220 MtrunA17_Chr1g0200071 100.000 448 0 0 1 448 1 448 0.0 922
Msa0177220 MtrunA17_Chr1g0200091 100.000 448 0 0 1 448 1 448 0.0 922
Msa0177220 MtrunA17_Chr1g0200121 99.777 448 1 0 1 448 1 448 0.0 921
Msa0177220 MtrunA17_Chr6g0458111 96.652 448 14 1 1 448 1 447 0.0 895
Msa0177220 MtrunA17_Chr6g0458091 96.652 448 14 1 1 448 1 447 0.0 895
Msa0177220 MtrunA17_Chr4g0005781 96.429 448 15 1 1 448 1 447 0.0 890
Msa0177220 MtrunA17_Chr1g0150331 91.574 451 9 2 1 448 1 425 0.0 829
Msa0177220 MtrunA17_Chr1g0193591 82.719 434 75 0 1 434 1 434 0.0 744
Msa0177220 MtrunA17_Chr1g0204031 82.151 437 73 3 1 437 1 432 0.0 736
Msa0177220 MtrunA17_Chr1g0156421 79.263 434 90 0 1 434 1 434 0.0 728
Msa0177220 MtrunA17_Chr8g0340771 79.955 449 88 1 1 447 1 449 0.0 723
Msa0177220 MtrunA17_Chr5g0415391 73.048 397 107 0 39 435 1 397 0.0 597
Msa0177220 MtrunA17_Chr1g0196251 69.727 403 94 2 4 405 5 380 0.0 576
Msa0177220 MtrunA17_Chr8g0336511 78.593 327 69 1 1 326 66 392 0.0 543
Msa0177220 MtrunA17_Chr2g0319341 72.254 346 93 2 102 445 1 345 0.0 517
Msa0177220 MtrunA17_Chr2g0298371 68.923 325 82 2 102 424 1 308 3.20e-161 457
Msa0177220 MtrunA17_Chr8g0340371 70.124 241 69 2 1 240 1 239 1.91e-118 345
Msa0177220 MtrunA17_Chr1g0210931 63.087 298 56 4 155 447 1 249 5.74e-118 345
Msa0177220 MtrunA17_Chr2g0318291 71.681 226 59 3 1 223 1 224 2.50e-112 329
Msa0177220 MtrunA17_Chr6g0483121 37.963 432 254 6 5 428 89 514 3.35e-97 301
Msa0177220 MtrunA17_Chr8g0392641 37.963 432 258 6 4 429 143 570 2.46e-92 290
Msa0177220 MtrunA17_Chr8g0380091 36.028 433 268 6 7 433 275 704 8.55e-92 292
Msa0177220 MtrunA17_Chr1g0204041 86.452 155 21 0 1 155 1 155 1.05e-90 272
Msa0177220 MtrunA17_Chr1g0200111 76.271 177 32 1 264 430 1 177 1.04e-87 265
Msa0177220 MtrunA17_Chr2g0318281 68.421 190 60 0 243 432 5 194 3.08e-84 258
Msa0177220 MtrunA17_Chr1g0156411 81.429 140 26 0 6 145 10 149 9.94e-79 241
Msa0177220 MtrunA17_Chr8g0340361 60.541 185 57 2 264 433 1 184 2.27e-66 210
Msa0177220 MtrunA17_Chr4g0026961 54.639 194 72 4 195 385 32 212 9.25e-59 192
Msa0177220 MtrunA17_Chr4g0026961 76.190 42 10 0 1 42 1 42 6.34e-13 67.8
Msa0177220 MtrunA17_Chr2g0298381 77.778 99 22 0 1 99 1 99 6.32e-48 159
Msa0177220 MtrunA17_Chr8g0341021 76.471 102 24 0 3 104 4 105 1.17e-47 159
Msa0177220 MtrunA17_Chr7g0259481 30.365 438 245 14 3 429 59 447 1.16e-45 164
Msa0177220 MtrunA17_Chr3g0116821 29.955 444 250 15 3 429 88 487 6.59e-41 152
Msa0177220 MtrunA17_Chr1g0211481 79.747 79 16 0 5 83 4 82 3.54e-36 129
Msa0177220 MtrunA17_Chr2g0319351 82.192 73 13 0 5 77 5 77 4.27e-35 125
Msa0177220 MtrunA17_Chr1g0200101 89.231 65 7 0 172 236 49 113 7.74e-35 125
Msa0177220 MtrunA17_Chr1g0200101 73.469 49 13 0 21 69 2 50 8.71e-17 76.3
Msa0177220 MtrunA17_Chr2g0287231 32.389 247 135 6 1 244 10 227 8.03e-31 119
Msa0177220 MtrunA17_Chr8g0341041 35.000 220 80 5 213 432 2 158 7.54e-29 111
Msa0177220 MtrunA17_Chr7g0261801 69.697 66 17 1 155 220 1 63 3.98e-24 95.9
Msa0177220 MtrunA17_Chr3g0130991 26.471 272 143 9 9 272 85 307 1.10e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177220 AT1G07920.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07940.4 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07940.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07940.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07940.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT5G60390.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT5G60390.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT5G60390.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07930.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177220 AT1G07930.2 98.824 255 3 0 1 255 1 255 0.0 530
Msa0177220 AT1G07930.2 87.179 117 14 1 331 447 254 369 9.10e-65 213
Msa0177220 AT1G18070.1 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa0177220 AT1G18070.2 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa0177220 AT1G18070.3 36.927 436 256 6 5 424 99 531 3.87e-91 286
Msa0177220 AT5G10630.1 35.765 425 265 5 7 426 239 660 1.08e-90 289
Msa0177220 AT5G10630.5 35.765 425 265 5 7 426 240 661 1.11e-90 289
Msa0177220 AT5G10630.2 35.765 425 265 5 7 426 240 661 1.11e-90 289
Msa0177220 AT5G10630.4 35.765 425 265 5 7 426 315 736 6.71e-90 289
Msa0177220 AT5G10630.3 35.765 425 265 5 7 426 310 731 7.31e-90 288
Msa0177220 AT1G35550.1 78.641 103 22 0 335 437 1 103 1.47e-53 174
Msa0177220 AT4G02930.1 30.296 439 245 15 3 429 63 452 8.29e-44 159
Msa0177220 AT4G20360.1 30.137 438 257 10 3 429 75 474 9.60e-44 160
Msa0177220 AT4G20360.2 30.137 438 257 10 3 429 75 474 9.60e-44 160
Msa0177220 AT2G31060.3 25.000 264 160 8 9 263 61 295 3.24e-12 68.9
Msa0177220 AT5G08650.2 26.667 270 145 10 9 272 88 310 2.83e-11 66.2
Msa0177220 AT5G08650.1 26.667 270 145 10 9 272 88 310 2.86e-11 66.2
Msa0177220 AT2G31060.2 24.615 260 162 7 9 263 61 291 3.81e-11 65.9

Find 180 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACCATTCTTCAAGAATT+TGG 0.066665 1_4:+64118780 None:intergenic
ATCATAGTTATTACAATAAA+TGG 0.259832 1_4:-64118534 Msa0177220:three_prime_UTR
TGCTGGGGCATGGTTCTATA+TGG 0.275303 1_4:-64118233 Msa0177220:three_prime_UTR
CTCCTTCTCAATTTCCTTAC+CGG 0.285465 1_4:+64118804 None:intergenic
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 1_4:-64119604 Msa0177220:CDS
GTCTACAAGATTGGAGGAAT+TGG 0.315206 1_4:-64119195 Msa0177220:CDS
GCCTCTTAGGCTCATTGATT+TGG 0.315866 1_4:+64119253 None:intergenic
TTATTACAATAAATGGTCTT+TGG 0.319176 1_4:-64118527 Msa0177220:three_prime_UTR
TGTCAAGAATGTTGCTGTTA+AGG 0.325240 1_4:-64119019 Msa0177220:CDS
TCACCATTCTTCAAGAATTT+GGG 0.331757 1_4:+64118781 None:intergenic
ATAGCCAACCTTCTTCAAAT+AGG 0.332380 1_4:+64119389 None:intergenic
AAGTTAGCAGCCTCCTTGGC+TGG 0.333938 1_4:+64118955 None:intergenic
ACCTCGTGAAGAAGGGAAAA+AGG 0.344425 1_4:+64121307 None:intergenic
CTTCTTCTTCAATGCTGACT+TGG 0.345006 1_4:+64118594 None:intergenic
CACATCAACATTGTGGTCAT+TGG 0.354316 1_4:-64119994 Msa0177220:CDS
GAGGTGTGGCAGTCAAGAAC+AGG 0.357372 1_4:+64118877 None:intergenic
ATTCCATTTGTTCCCATCTC+TGG 0.368209 1_4:-64119354 Msa0177220:CDS
GCTCTCACAGAGGCCCTTCC+TGG 0.369900 1_4:-64119060 Msa0177220:CDS
CTCCTTGGCTGGGTCATCCT+TGG 0.371873 1_4:+64118966 None:intergenic
GTAAGCAAGCAGTAAGTTCC+AGG 0.376045 1_4:-64118328 Msa0177220:three_prime_UTR
ATATCTACCCTTGGAGTACT+TGG 0.380807 1_4:+64119437 None:intergenic
TCACCATTGACATTGCTCTT+TGG 0.386025 1_4:-64119801 Msa0177220:CDS
GTCATTGGCCATGTCGACTC+TGG 0.395247 1_4:-64119979 Msa0177220:CDS
TGCGTCAAACTGTAGCTGTT+GGG 0.401918 1_4:-64118666 Msa0177220:CDS
CCTGTGGGGCGTGTTGAAAC+TGG 0.405151 1_4:-64119165 Msa0177220:CDS
GCAAAAGTGACAACCATTCC+AGG 0.418006 1_4:+64119132 None:intergenic
CTCATCATTGATTCCACTAC+TGG 0.419293 1_4:-64119673 Msa0177220:CDS
ATGAATCACCCTGGCCAGAT+TGG 0.425183 1_4:-64118913 Msa0177220:CDS
TCATTCCTTGCTACTCGACT+AGG 0.426624 1_4:-64121345 Msa0177220:five_prime_UTR
CTTAATGAAATCCCTGTGTC+CGG 0.429384 1_4:+64119735 None:intergenic
CACCCTGGCCAGATTGGAAA+TGG 0.432985 1_4:-64118907 Msa0177220:CDS
GCCCCTCCGACTACCACTTC+AGG 0.433104 1_4:-64119220 Msa0177220:CDS
ACTAAGATTGATAGGCGTTC+CGG 0.433848 1_4:-64118823 Msa0177220:CDS
TGACAAGCTTAAGGCTGAAC+GGG 0.436450 1_4:-64119833 Msa0177220:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 1_4:-64120019 Msa0177220:exon
AGCGTTCATTCAAGTATGCC+TGG 0.444655 1_4:-64119861 Msa0177220:CDS
GGCTTGTCTGAAGGCCTCTT+AGG 0.446650 1_4:+64119240 None:intergenic
GGGCATAGCCATTTCCAATC+TGG 0.448022 1_4:+64118899 None:intergenic
AAACCAGAGATGGGAACAAA+TGG 0.449170 1_4:+64119351 None:intergenic
GGTCTGCTTAAGTCCTCCAC+TGG 0.451209 1_4:+64118615 None:intergenic
GGCATGGTTCTATATGGTTT+AGG 0.454376 1_4:-64118227 Msa0177220:three_prime_UTR
ACTTCAGTTGTCAGTCCAGT+AGG 0.456008 1_4:+64119108 None:intergenic
CTTCAGGATGTCTACAAGAT+TGG 0.458119 1_4:-64119204 Msa0177220:CDS
AGTCTCAACCACCATGGGCT+TGG 0.462350 1_4:+64118732 None:intergenic
ACCAAATCAATGAGCCTAAG+AGG 0.463105 1_4:-64119254 Msa0177220:CDS
CTGGGTCCTTGACAAGCTTA+AGG 0.464518 1_4:-64119842 Msa0177220:CDS
TGGGTCATCCTTGGAGTTTG+AGG 0.465051 1_4:+64118975 None:intergenic
CACAAACCTTGACTGGTACA+AGG 0.466653 1_4:-64119301 Msa0177220:CDS
CTCAACCACCATGGGCTTGG+TGG 0.468734 1_4:+64118735 None:intergenic
GGGTTGTTACCTCGTGAAGA+AGG 0.471526 1_4:+64121299 None:intergenic
TAGGCTCATTGATTTGGTCA+AGG 0.472707 1_4:+64119259 None:intergenic
TTAATGAAATCCCTGTGTCC+GGG 0.473933 1_4:+64119736 None:intergenic
TAGCCATTTCCAATCTGGCC+AGG 0.474048 1_4:+64118904 None:intergenic
AGGCTCATTGATTTGGTCAA+GGG 0.475242 1_4:+64119260 None:intergenic
TGTAAAGTTAGCAGCCTCCT+TGG 0.477279 1_4:+64118951 None:intergenic
AGCTTGTTACTAAGATTGAT+AGG 0.481965 1_4:-64118831 Msa0177220:CDS
ACAATCAGCTTGGGATGTTC+CGG 0.483792 1_4:+64119702 None:intergenic
GACGAGAAATAGAAATACTA+AGG 0.487881 1_4:+64118503 None:intergenic
TCATCCTATTTGAAGAAGGT+TGG 0.491235 1_4:-64119393 Msa0177220:CDS
CTTGTACCAGTCAAGGTTTG+TGG 0.492698 1_4:+64119302 None:intergenic
GAGAATAGAGAGGGACGGTA+GGG 0.495192 1_4:+64121375 None:intergenic
ATCTCCCTCAAAACCAGAGA+TGG 0.496573 1_4:+64119341 None:intergenic
GGGTTTATAAACTAGTACTC+TGG 0.498858 1_4:+64121418 None:intergenic
CATCAAGGCTGTGGAGAAGA+AGG 0.499167 1_4:-64118641 Msa0177220:CDS
GAAACAGAGTCAGCAAGTCC+TGG 0.500128 1_4:+64118310 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 1_4:+64119639 None:intergenic
AGTTTCATCCTATTTGAAGA+AGG 0.501588 1_4:-64119397 Msa0177220:CDS
ATCATTGATTCCACTACTGG+TGG 0.502099 1_4:-64119670 Msa0177220:CDS
CTTCAGTTGTCAGTCCAGTA+GGG 0.506099 1_4:+64119109 None:intergenic
GTTGTGTCTAGTCAGAAACT+TGG 0.507294 1_4:-64118356 Msa0177220:three_prime_UTR
AATTTCATCATATCTACCCT+TGG 0.508007 1_4:+64119428 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 1_4:+64119765 None:intergenic
AGCAGGATGATCTATCTACC+TGG 0.510712 1_4:-64118564 Msa0177220:three_prime_UTR
CATTCCTTGCTACTCGACTA+GGG 0.514565 1_4:-64121344 Msa0177220:five_prime_UTR
TGAGAACGGCACAATCAGCT+TGG 0.516820 1_4:+64119692 None:intergenic
AGGAATTGGAACTGTGCCTG+TGG 0.517140 1_4:-64119181 Msa0177220:CDS
TGAGAATAGAGAGGGACGGT+AGG 0.520249 1_4:+64121374 None:intergenic
GAAGAAGAAGTGAACCGAGC+AGG 0.523086 1_4:-64118581 Msa0177220:exon
CAGGATGTCTACAAGATTGG+AGG 0.523217 1_4:-64119201 Msa0177220:CDS
GAACAAAAGCTAAGAGATGA+AGG 0.523844 1_4:+64118442 None:intergenic
CTTAAGGCTGAACGGGAAAG+AGG 0.523952 1_4:-64119826 Msa0177220:CDS
GAGCCCAAATTCTTGAAGAA+TGG 0.528480 1_4:-64118784 Msa0177220:CDS
ATGCGTCAAACTGTAGCTGT+TGG 0.528651 1_4:-64118667 Msa0177220:CDS
TCCCACATTGTCACCAGGAA+GGG 0.528721 1_4:+64119047 None:intergenic
AGTCGGAGGGGCTTGTCTGA+AGG 0.529275 1_4:+64119231 None:intergenic
GAGAACGGCACAATCAGCTT+GGG 0.529995 1_4:+64119693 None:intergenic
GATTGATAGGCGTTCCGGTA+AGG 0.530821 1_4:-64118818 Msa0177220:CDS
CTTCCAAAGAGCAATGTCAA+TGG 0.534369 1_4:+64119798 None:intergenic
GGAATTGGAACTGTGCCTGT+GGG 0.536194 1_4:-64119180 Msa0177220:CDS
AGCAAACTTGACAGCAATGT+GGG 0.541075 1_4:+64118855 None:intergenic
TGTAGCTGTTGGGGTCATCA+AGG 0.544261 1_4:-64118656 Msa0177220:CDS
TGCACTGTCATTGATGCCCC+CGG 0.544718 1_4:-64119754 Msa0177220:CDS
CACTTGATCTACAAGCTTGG+AGG 0.545798 1_4:-64119937 Msa0177220:CDS
GTCTGCTTAAGTCCTCCACT+GGG 0.549538 1_4:+64118616 None:intergenic
GGCTTCAAAACCACCAGTAG+TGG 0.551737 1_4:+64119660 None:intergenic
GGTCACTTGATCTACAAGCT+TGG 0.555547 1_4:-64119940 Msa0177220:CDS
TCAAGGGCCTCAAGAAGGGT+TGG 0.557590 1_4:+64119276 None:intergenic
TCAACCACCATGGGCTTGGT+GGG 0.557624 1_4:+64118736 None:intergenic
TTGGTCAAGGGCCTCAAGAA+GGG 0.558003 1_4:+64119272 None:intergenic
TTGGACCCTTGTACCAGTCA+AGG 0.558366 1_4:+64119295 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 1_4:+64119614 None:intergenic
TCATTGGCCATGTCGACTCT+GGG 0.559180 1_4:-64119978 Msa0177220:CDS
AGCGCTCCACAAACCTTGAC+TGG 0.559246 1_4:-64119308 Msa0177220:CDS
AGGTGTGGCAGTCAAGAACA+GGG 0.559985 1_4:+64118878 None:intergenic
ATAACTATGATATGCTTCCC+AGG 0.560332 1_4:+64118546 None:intergenic
TTTGGTCAAGGGCCTCAAGA+AGG 0.560467 1_4:+64119271 None:intergenic
TTCTTGAAGAATGGTGATGC+TGG 0.561159 1_4:-64118775 Msa0177220:CDS
AGTTAGCAGCCTCCTTGGCT+GGG 0.563218 1_4:+64118956 None:intergenic
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 1_4:-64119643 Msa0177220:CDS
TCTGGGAAATCAACCACCAC+TGG 0.564990 1_4:-64119961 Msa0177220:CDS
ACAAACCTTGACTGGTACAA+GGG 0.567625 1_4:-64119300 Msa0177220:CDS
GGTTGTTACCTCGTGAAGAA+GGG 0.568985 1_4:+64121300 None:intergenic
TAATGAAATCCCTGTGTCCG+GGG 0.570266 1_4:+64119737 None:intergenic
TTCATTAAGAACATGATCAC+CGG 0.572337 1_4:-64119721 Msa0177220:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 1_4:+64119615 None:intergenic
CTTAAGCTTGTCAAGGACCC+AGG 0.575518 1_4:+64119843 None:intergenic
GAGAAAGTCTCAACCACCAT+GGG 0.575954 1_4:+64118727 None:intergenic
ATCCTGAAGTGGTAGTCGGA+GGG 0.576252 1_4:+64119218 None:intergenic
CCAGTTTCAACACGCCCCAC+AGG 0.580692 1_4:+64119165 None:intergenic
TATCTACCCTTGGAGTACTT+GGG 0.580849 1_4:+64119438 None:intergenic
GCGTCAAACTGTAGCTGTTG+GGG 0.581681 1_4:-64118665 Msa0177220:CDS
ATCCCACATTGTCACCAGGA+AGG 0.585536 1_4:+64119046 None:intergenic
GGCCCTTCCTGGTGACAATG+TGG 0.586985 1_4:-64119049 Msa0177220:CDS
TTGATTTCCCAGAGTCGACA+TGG 0.588973 1_4:+64119971 None:intergenic
CTGTTAAGGATCTCAAGCGT+GGG 0.589274 1_4:-64119005 Msa0177220:CDS
TTCTCTGAATATCCTCCACT+CGG 0.591172 1_4:-64118709 Msa0177220:CDS
GCCCTTCCTGGTGACAATGT+GGG 0.592402 1_4:-64119048 Msa0177220:CDS
TTGACAAGCTTAAGGCTGAA+CGG 0.594016 1_4:-64119834 Msa0177220:CDS
TGTTGGGGTCATCAAGGCTG+TGG 0.596509 1_4:-64118650 Msa0177220:CDS
TGGTGTCATAAAACCTGGAA+TGG 0.598505 1_4:-64119145 Msa0177220:CDS
TAGATATGATGAAATTGTGA+AGG 0.599770 1_4:-64119421 Msa0177220:CDS
GCATGTCTCTAACAGCAAAA+CGG 0.600985 1_4:+64118686 None:intergenic
CTCCAAGGATGACCCAGCCA+AGG 0.602045 1_4:-64118968 Msa0177220:CDS
GCGTTCATTCAAGTATGCCT+GGG 0.605879 1_4:-64119860 Msa0177220:CDS
TCTCCCTCAAAACCAGAGAT+GGG 0.607478 1_4:+64119342 None:intergenic
TCCTGAAGTGGTAGTCGGAG+GGG 0.608492 1_4:+64119219 None:intergenic
GATGATTCCCACCAAGCCCA+TGG 0.611182 1_4:-64118743 Msa0177220:CDS
TACTACACCCAAGTACTCCA+AGG 0.613862 1_4:-64119445 Msa0177220:CDS
GTTCAGCCTTAAGCTTGTCA+AGG 0.618150 1_4:+64119836 None:intergenic
CAAGGGTCCAACCCTTCTTG+AGG 0.620566 1_4:-64119283 Msa0177220:CDS
TCACACCAAGAGTGAAAGCA+AGG 0.621193 1_4:+64119599 None:intergenic
ATCTTGTAGACATCCTGAAG+TGG 0.621388 1_4:+64119207 None:intergenic
GCTGTTAAGGATCTCAAGCG+TGG 0.622485 1_4:-64119006 Msa0177220:CDS
TTCCGGTAAGGAAATTGAGA+AGG 0.622677 1_4:-64118806 Msa0177220:CDS
TTCAGTTGTCAGTCCAGTAG+GGG 0.622733 1_4:+64119110 None:intergenic
AGCCATTTCCAATCTGGCCA+GGG 0.623271 1_4:+64118905 None:intergenic
GAAACTGGTGTCATAAAACC+TGG 0.623591 1_4:-64119150 Msa0177220:CDS
GACAACTGAAGTCAAGTCTG+TGG 0.624055 1_4:-64119097 Msa0177220:CDS
ACAGCAAAACGGCCGAGTGG+AGG 0.624787 1_4:+64118697 None:intergenic
GCAGGATGATCTATCTACCT+GGG 0.625090 1_4:-64118563 Msa0177220:three_prime_UTR
TTGACAAGCGTGTCATCGAG+AGG 0.625348 1_4:-64119912 Msa0177220:CDS
GTATGTTGCCTCAAACTCCA+AGG 0.633897 1_4:-64118983 Msa0177220:CDS
CATCCTGAAGTGGTAGTCGG+AGG 0.637451 1_4:+64119217 None:intergenic
AGACATCCTGAAGTGGTAGT+CGG 0.638051 1_4:+64119214 None:intergenic
CAGCAAACTTGACAGCAATG+TGG 0.638328 1_4:+64118854 None:intergenic
AGAACTGGGTTCTAGATCGA+CGG 0.639454 1_4:-64118417 Msa0177220:three_prime_UTR
AGAGAAAGTCTCAACCACCA+TGG 0.641605 1_4:+64118726 None:intergenic
GGTAGATAGATCATCCTGCT+CGG 0.643375 1_4:+64118567 None:intergenic
GAATTGGAACTGTGCCTGTG+GGG 0.644316 1_4:-64119179 Msa0177220:CDS
AGTGGAATCAATGATGAGAA+CGG 0.644693 1_4:+64119678 None:intergenic
TGACAGCAATGTGGGAGGTG+TGG 0.650950 1_4:+64118863 None:intergenic
GAAACCCTAGTCGAGTAGCA+AGG 0.651284 1_4:+64121340 None:intergenic
TTAAATCCCACATTGTCACC+AGG 0.657091 1_4:+64119042 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 1_4:-64119572 Msa0177220:intron
CAAGGATGACCCAGCCAAGG+AGG 0.659665 1_4:-64118965 Msa0177220:CDS
AATGAAATCCCTGTGTCCGG+GGG 0.661737 1_4:+64119738 None:intergenic
GAGAAGAAGGATCCCAGTGG+AGG 0.667988 1_4:-64118628 Msa0177220:CDS
AAAGGTTCACATCAACATTG+TGG 0.675059 1_4:-64120001 Msa0177220:CDS
ACTACACCCAAGTACTCCAA+GGG 0.678033 1_4:-64119444 Msa0177220:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 1_4:+64119945 None:intergenic
CTAACAGCAAAACGGCCGAG+TGG 0.692072 1_4:+64118694 None:intergenic
AAACTTGACAGCAATGTGGG+AGG 0.695398 1_4:+64118858 None:intergenic
GTAGATCAAGTGACCAGTGG+TGG 0.699002 1_4:+64119948 None:intergenic
GTGATCATAATGAATCACCC+TGG 0.699056 1_4:-64118922 Msa0177220:CDS
GGTGTGGCAGTCAAGAACAG+GGG 0.711459 1_4:+64118879 None:intergenic
GATTCCCACCAAGCCCATGG+TGG 0.723849 1_4:-64118740 Msa0177220:CDS
AAAGCTTAAACATCACACCA+AGG 0.724807 1_4:+64118256 None:intergenic
ACTGGGTTCTAGATCGACGG+TGG 0.727144 1_4:-64118414 Msa0177220:three_prime_UTR
GTGGAGAAGAAGGATCCCAG+TGG 0.734762 1_4:-64118631 Msa0177220:CDS
GCATCATGAAGCTCTCACAG+AGG 0.758446 1_4:-64119070 Msa0177220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCATTTTTTTTTTACTT+TGG - chr1_4:64119294-64119313 Msa0177220:CDS 10.0%
!!! ATTTAATTTTTTTCTTTCTT+TGG - chr1_4:64119542-64119561 Msa0177220:intron 10.0%
!!! TTTAATTTTTTTCTTTCTTT+GGG - chr1_4:64119543-64119562 Msa0177220:intron 10.0%
!!! TATGAAATTTAGTTTCAATT+TGG - chr1_4:64118768-64118787 Msa0177220:CDS 15.0%
!!! AATCCTTTATGTTTTTAAAT+AGG - chr1_4:64118862-64118881 Msa0177220:CDS 15.0%
!!! TTAATTTTTTTCTTTCTTTG+GGG - chr1_4:64119544-64119563 Msa0177220:intron 15.0%
!! ATCATAGTTATTACAATAAA+TGG - chr1_4:64121106-64121125 Msa0177220:intron 15.0%
!!! TGTTATATACTTCTTTTGTT+TGG - chr1_4:64118656-64118675 Msa0177220:CDS 20.0%
!! TAAATTTCATAACGTAAAAC+AGG + chr1_4:64118759-64118778 None:intergenic 20.0%
!! ATTCAGAATTACAATTAAAC+AGG + chr1_4:64118828-64118847 None:intergenic 20.0%
!! ATCTTGAATTCATTTATGTA+TGG - chr1_4:64120139-64120158 Msa0177220:intron 20.0%
!! TTATTACAATAAATGGTCTT+TGG - chr1_4:64121113-64121132 Msa0177220:intron 20.0%
!!! TTATTTTTGTGTTTATTCCT+TGG - chr1_4:64121367-64121386 Msa0177220:five_prime_UTR 20.0%
!! TTTTAATATAAGGGTTTCAG+GGG + chr1_4:64118245-64118264 None:intergenic 25.0%
!!! GTTTTAATATAAGGGTTTCA+GGG + chr1_4:64118246-64118265 None:intergenic 25.0%
!!! AAACGAAATTGATGGATTTA+GGG + chr1_4:64118364-64118383 None:intergenic 25.0%
! CATATCATCAATTCATTCAT+AGG - chr1_4:64118406-64118425 Msa0177220:three_prime_UTR 25.0%
! CTGATAAATAAAAGAACGAT+TGG + chr1_4:64118530-64118549 None:intergenic 25.0%
!!! CTGTTTTAGTGTAATTACAT+AGG - chr1_4:64118716-64118735 Msa0177220:CDS 25.0%
!! TACATAGGATCATTTGAAAT+TGG - chr1_4:64118731-64118750 Msa0177220:CDS 25.0%
!! TAAAGGATTATCACACTTTT+TGG + chr1_4:64118851-64118870 None:intergenic 25.0%
! CCACCTATTTAAAAACATAA+AGG + chr1_4:64118868-64118887 None:intergenic 25.0%
!!! CCTTTATGTTTTTAAATAGG+TGG - chr1_4:64118865-64118884 Msa0177220:CDS 25.0%
!!! TTGTGAAGGTTTTAACTTTT+TGG - chr1_4:64118898-64118917 Msa0177220:CDS 25.0%
! AGTTAGTAGTTATTTGTTTC+TGG - chr1_4:64118989-64119008 Msa0177220:CDS 25.0%
!! TTGTTCTCCTATTTTATTCT+TGG - chr1_4:64119057-64119076 Msa0177220:CDS 25.0%
! TCAGTAACCAAGAATAAAAT+AGG + chr1_4:64119067-64119086 None:intergenic 25.0%
! TCTGATCTTATCTATTAATC+AGG + chr1_4:64119134-64119153 None:intergenic 25.0%
! CAAAAGAAGAAGAAGAAAAA+GGG + chr1_4:64119236-64119255 None:intergenic 25.0%
! TCAAAAGAAGAAGAAGAAAA+AGG + chr1_4:64119237-64119256 None:intergenic 25.0%
! CAAAATGTTATCCCATAAAT+GGG + chr1_4:64119430-64119449 None:intergenic 25.0%
! ACAAAATGTTATCCCATAAA+TGG + chr1_4:64119431-64119450 None:intergenic 25.0%
! TAGATATGATGAAATTGTGA+AGG - chr1_4:64120219-64120238 Msa0177220:intron 25.0%
!! GGTTTTAATATAAGGGTTTC+AGG + chr1_4:64118247-64118266 None:intergenic 30.0%
!! ACGGTAGGGTTTTAATATAA+GGG + chr1_4:64118254-64118273 None:intergenic 30.0%
!! GAGAATTTTGAGAATAGAGA+GGG + chr1_4:64118277-64118296 None:intergenic 30.0%
!! TGAGAATTTTGAGAATAGAG+AGG + chr1_4:64118278-64118297 None:intergenic 30.0%
!! GAAACGAAATTGATGGATTT+AGG + chr1_4:64118365-64118384 None:intergenic 30.0%
TCGATAAGAAACGAAATTGA+TGG + chr1_4:64118372-64118391 None:intergenic 30.0%
!! TTTTATTTATCAGTCATCGC+TGG - chr1_4:64118537-64118556 Msa0177220:three_prime_UTR 30.0%
AGGATGATATAATAATCGCA+TGG + chr1_4:64118609-64118628 None:intergenic 30.0%
CTCAAAAAAAGAACAACGTA+AGG + chr1_4:64118629-64118648 None:intergenic 30.0%
!! TTCTTTTGTTTGGTATTCCT+TGG - chr1_4:64118666-64118685 Msa0177220:CDS 30.0%
TTGCAAAAAAACAGAATCCA+AGG + chr1_4:64118686-64118705 None:intergenic 30.0%
ATGAAATATGATGTTTCAGC+AGG + chr1_4:64119017-64119036 None:intergenic 30.0%
! TGCTCATGTATTATTTTCCT+TGG - chr1_4:64119570-64119589 Msa0177220:intron 30.0%
! TTTGTGGTTTTCAGCTTTTA+AGG - chr1_4:64119601-64119620 Msa0177220:CDS 30.0%
!! TTTCAGCTTTTAAGGACTAA+TGG - chr1_4:64119609-64119628 Msa0177220:CDS 30.0%
!! TTCAGCTTTTAAGGACTAAT+GGG - chr1_4:64119610-64119629 Msa0177220:CDS 30.0%
TTCATTAAGAACATGATCAC+CGG - chr1_4:64119919-64119938 Msa0177220:CDS 30.0%
AATTTCATCATATCTACCCT+TGG + chr1_4:64120215-64120234 None:intergenic 30.0%
AGTTTCATCCTATTTGAAGA+AGG - chr1_4:64120243-64120262 Msa0177220:intron 30.0%
! GAACAAATGGAATTTTGTCT+GGG + chr1_4:64120279-64120298 None:intergenic 30.0%
!! AGCTTGTTACTAAGATTGAT+AGG - chr1_4:64120809-64120828 Msa0177220:intron 30.0%
TCACCATTCTTCAAGAATTT+GGG + chr1_4:64120862-64120881 None:intergenic 30.0%
ATCACCATTCTTCAAGAATT+TGG + chr1_4:64120863-64120882 None:intergenic 30.0%
GACGAGAAATAGAAATACTA+AGG + chr1_4:64121140-64121159 None:intergenic 30.0%
GGGTTTATAAACTAGTACTC+TGG + chr1_4:64118225-64118244 None:intergenic 35.0%
!! GACGGTAGGGTTTTAATATA+AGG + chr1_4:64118255-64118274 None:intergenic 35.0%
!! ATTTTGAGAATAGAGAGGGA+CGG + chr1_4:64118273-64118292 None:intergenic 35.0%
!! GTGGTTTTACTTTGTTGTGA+AGG - chr1_4:64118884-64118903 Msa0177220:CDS 35.0%
ACAACAACAATATGTCAGCA+GGG + chr1_4:64119041-64119060 None:intergenic 35.0%
AACAACAACAATATGTCAGC+AGG + chr1_4:64119042-64119061 None:intergenic 35.0%
GATTCTTCAGCAGATATAAC+AGG + chr1_4:64119101-64119120 None:intergenic 35.0%
! CATCAGTCTTGTAAAACTGA+AGG - chr1_4:64119354-64119373 Msa0177220:CDS 35.0%
ATTGTTGCCAAACCCATTTA+TGG - chr1_4:64119415-64119434 Msa0177220:CDS 35.0%
TTGTTGCCAAACCCATTTAT+GGG - chr1_4:64119416-64119435 Msa0177220:CDS 35.0%
! TGTTATCCCATAAATGGGTT+TGG + chr1_4:64119425-64119444 None:intergenic 35.0%
!! CTTTTAAGGACTAATGGGTA+AGG - chr1_4:64119615-64119634 Msa0177220:CDS 35.0%
AAAGGTTCACATCAACATTG+TGG - chr1_4:64119639-64119658 Msa0177220:CDS 35.0%
AGTGGAATCAATGATGAGAA+CGG + chr1_4:64119965-64119984 None:intergenic 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG - chr1_4:64119993-64120012 Msa0177220:CDS 35.0%
ATCCATCTGTTCAAGATAGA+TGG + chr1_4:64120173-64120192 None:intergenic 35.0%
TCATCCTATTTGAAGAAGGT+TGG - chr1_4:64120247-64120266 Msa0177220:intron 35.0%
ATAGCCAACCTTCTTCAAAT+AGG + chr1_4:64120254-64120273 None:intergenic 35.0%
! GGAACAAATGGAATTTTGTC+TGG + chr1_4:64120280-64120299 None:intergenic 35.0%
! TGTCAAGAATGTTGCTGTTA+AGG - chr1_4:64120621-64120640 Msa0177220:intron 35.0%
ATAACTATGATATGCTTCCC+AGG + chr1_4:64121097-64121116 None:intergenic 35.0%
GAACAAAAGCTAAGAGATGA+AGG + chr1_4:64121201-64121220 None:intergenic 35.0%
!! TTAGCTTTTGTTCTCAGAAC+TGG - chr1_4:64121208-64121227 Msa0177220:intron 35.0%
! TAGCTTTTGTTCTCAGAACT+GGG - chr1_4:64121209-64121228 Msa0177220:intron 35.0%
AAAGCTTAAACATCACACCA+AGG + chr1_4:64121387-64121406 None:intergenic 35.0%
! TGTGATGTTTAAGCTTTTGC+TGG - chr1_4:64121390-64121409 Msa0177220:five_prime_UTR 35.0%
! GTGATGTTTAAGCTTTTGCT+GGG - chr1_4:64121391-64121410 Msa0177220:five_prime_UTR 35.0%
! TGATGTTTAAGCTTTTGCTG+GGG - chr1_4:64121392-64121411 Msa0177220:five_prime_UTR 35.0%
CAACAACAATATGTCAGCAG+GGG + chr1_4:64119040-64119059 None:intergenic 40.0%
TCAAATGGAGCCCTTTAACT+CGG - chr1_4:64119188-64119207 Msa0177220:CDS 40.0%
TACTCTACCACCGAGTTAAA+GGG + chr1_4:64119201-64119220 None:intergenic 40.0%
TTACTCTACCACCGAGTTAA+AGG + chr1_4:64119202-64119221 None:intergenic 40.0%
AAGAAGAAAAAGGGACTCCA+CGG + chr1_4:64119227-64119246 None:intergenic 40.0%
! GTCAACAACACACTGACTAA+AGG + chr1_4:64119511-64119530 None:intergenic 40.0%
! TTAGTCAGTGTGTTGTTGAC+AGG - chr1_4:64119511-64119530 Msa0177220:intron 40.0%
! TTCCTTGGCAGATCTTTTTG+TGG - chr1_4:64119585-64119604 Msa0177220:CDS 40.0%
AACCACAAAAAGATCTGCCA+AGG + chr1_4:64119590-64119609 None:intergenic 40.0%
! AGGACTAATGGGTAAGGAAA+AGG - chr1_4:64119621-64119640 Msa0177220:CDS 40.0%
CACATCAACATTGTGGTCAT+TGG - chr1_4:64119646-64119665 Msa0177220:CDS 40.0%
TTGACAAGCTTAAGGCTGAA+CGG - chr1_4:64119806-64119825 Msa0177220:CDS 40.0%
TCACCATTGACATTGCTCTT+TGG - chr1_4:64119839-64119858 Msa0177220:CDS 40.0%
CTTCCAAAGAGCAATGTCAA+TGG + chr1_4:64119845-64119864 None:intergenic 40.0%
AATGACAGTGCAGTAGTACT+TGG + chr1_4:64119878-64119897 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+GGG + chr1_4:64119907-64119926 None:intergenic 40.0%
CTTAATGAAATCCCTGTGTC+CGG + chr1_4:64119908-64119927 None:intergenic 40.0%
!! CTCATCATTGATTCCACTAC+TGG - chr1_4:64119967-64119986 Msa0177220:CDS 40.0%
!! ATCATTGATTCCACTACTGG+TGG - chr1_4:64119970-64119989 Msa0177220:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG + chr1_4:64120004-64120023 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG - chr1_4:64120068-64120087 Msa0177220:intron 40.0%
TGCCATCTATCTTGAACAGA+TGG - chr1_4:64120168-64120187 Msa0177220:intron 40.0%
! TATCTACCCTTGGAGTACTT+GGG + chr1_4:64120205-64120224 None:intergenic 40.0%
! ATATCTACCCTTGGAGTACT+TGG + chr1_4:64120206-64120225 None:intergenic 40.0%
ATTCCATTTGTTCCCATCTC+TGG - chr1_4:64120286-64120305 Msa0177220:intron 40.0%
AAACCAGAGATGGGAACAAA+TGG + chr1_4:64120292-64120311 None:intergenic 40.0%
ACAAACCTTGACTGGTACAA+GGG - chr1_4:64120340-64120359 Msa0177220:intron 40.0%
!! AGGCTCATTGATTTGGTCAA+GGG + chr1_4:64120383-64120402 None:intergenic 40.0%
!! TAGGCTCATTGATTTGGTCA+AGG + chr1_4:64120384-64120403 None:intergenic 40.0%
ACCAAATCAATGAGCCTAAG+AGG - chr1_4:64120386-64120405 Msa0177220:intron 40.0%
ATCTTGTAGACATCCTGAAG+TGG + chr1_4:64120436-64120455 None:intergenic 40.0%
CTTCAGGATGTCTACAAGAT+TGG - chr1_4:64120436-64120455 Msa0177220:intron 40.0%
GTCTACAAGATTGGAGGAAT+TGG - chr1_4:64120445-64120464 Msa0177220:intron 40.0%
!! GAAACTGGTGTCATAAAACC+TGG - chr1_4:64120490-64120509 Msa0177220:intron 40.0%
!! TGGTGTCATAAAACCTGGAA+TGG - chr1_4:64120495-64120514 Msa0177220:intron 40.0%
TTAAATCCCACATTGTCACC+AGG + chr1_4:64120601-64120620 None:intergenic 40.0%
GTGATCATAATGAATCACCC+TGG - chr1_4:64120718-64120737 Msa0177220:intron 40.0%
AGCAAACTTGACAGCAATGT+GGG + chr1_4:64120788-64120807 None:intergenic 40.0%
ACTAAGATTGATAGGCGTTC+CGG - chr1_4:64120817-64120836 Msa0177220:intron 40.0%
TTCCGGTAAGGAAATTGAGA+AGG - chr1_4:64120834-64120853 Msa0177220:intron 40.0%
CTCCTTCTCAATTTCCTTAC+CGG + chr1_4:64120839-64120858 None:intergenic 40.0%
GAGCCCAAATTCTTGAAGAA+TGG - chr1_4:64120856-64120875 Msa0177220:intron 40.0%
! TTCTTGAAGAATGGTGATGC+TGG - chr1_4:64120865-64120884 Msa0177220:intron 40.0%
TTCTCTGAATATCCTCCACT+CGG - chr1_4:64120931-64120950 Msa0177220:intron 40.0%
GCATGTCTCTAACAGCAAAA+CGG + chr1_4:64120957-64120976 None:intergenic 40.0%
! CTTCTTCTTCAATGCTGACT+TGG + chr1_4:64121049-64121068 None:intergenic 40.0%
GTTGTGTCTAGTCAGAAACT+TGG - chr1_4:64121284-64121303 Msa0177220:intron 40.0%
! GGCATGGTTCTATATGGTTT+AGG - chr1_4:64121413-64121432 Msa0177220:five_prime_UTR 40.0%
TCATTCCTTGCTACTCGACT+AGG - chr1_4:64118295-64118314 Msa0177220:three_prime_UTR 45.0%
CATTCCTTGCTACTCGACTA+GGG - chr1_4:64118296-64118315 Msa0177220:three_prime_UTR 45.0%
ACCTCGTGAAGAAGGGAAAA+AGG + chr1_4:64118336-64118355 None:intergenic 45.0%
GGTTGTTACCTCGTGAAGAA+GGG + chr1_4:64118343-64118362 None:intergenic 45.0%
!!! TCAGTCATCGCTGGTTTTGA+AGG - chr1_4:64118546-64118565 Msa0177220:three_prime_UTR 45.0%
GACACACACATATGCTTGTC+CGG - chr1_4:64119157-64119176 Msa0177220:CDS 45.0%
ACACACACATATGCTTGTCC+GGG - chr1_4:64119158-64119177 Msa0177220:CDS 45.0%
AACTTGCCCTTATAGCTCAG+TGG - chr1_4:64119326-64119345 Msa0177220:CDS 45.0%
GCTCTACCACTGAGCTATAA+GGG + chr1_4:64119335-64119354 None:intergenic 45.0%
TGCTCTACCACTGAGCTATA+AGG + chr1_4:64119336-64119355 None:intergenic 45.0%
TTGATTTCCCAGAGTCGACA+TGG + chr1_4:64119672-64119691 None:intergenic 45.0%
CTTGTAGATCAAGTGACCAG+TGG + chr1_4:64119698-64119717 None:intergenic 45.0%
GGTCACTTGATCTACAAGCT+TGG - chr1_4:64119700-64119719 Msa0177220:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG - chr1_4:64119703-64119722 Msa0177220:CDS 45.0%
AGCGTTCATTCAAGTATGCC+TGG - chr1_4:64119779-64119798 Msa0177220:CDS 45.0%
GCGTTCATTCAAGTATGCCT+GGG - chr1_4:64119780-64119799 Msa0177220:CDS 45.0%
GTTCAGCCTTAAGCTTGTCA+AGG + chr1_4:64119807-64119826 None:intergenic 45.0%
TGACAAGCTTAAGGCTGAAC+GGG - chr1_4:64119807-64119826 Msa0177220:CDS 45.0%
TAATGAAATCCCTGTGTCCG+GGG + chr1_4:64119906-64119925 None:intergenic 45.0%
ACAATCAGCTTGGGATGTTC+CGG + chr1_4:64119941-64119960 None:intergenic 45.0%
!!! ACTACTGGTGGTTTTGAAGC+CGG - chr1_4:64119982-64120001 Msa0177220:CDS 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG - chr1_4:64119997-64120016 Msa0177220:CDS 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG + chr1_4:64120029-64120048 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG + chr1_4:64120044-64120063 None:intergenic 45.0%
TACTACACCCAAGTACTCCA+AGG - chr1_4:64120195-64120214 Msa0177220:intron 45.0%
ACTACACCCAAGTACTCCAA+GGG - chr1_4:64120196-64120215 Msa0177220:intron 45.0%
!!! TGTTCCCATCTCTGGTTTTG+AGG - chr1_4:64120294-64120313 Msa0177220:intron 45.0%
!!! GTTCCCATCTCTGGTTTTGA+GGG - chr1_4:64120295-64120314 Msa0177220:intron 45.0%
TCTCCCTCAAAACCAGAGAT+GGG + chr1_4:64120301-64120320 None:intergenic 45.0%
ATCTCCCTCAAAACCAGAGA+TGG + chr1_4:64120302-64120321 None:intergenic 45.0%
CTTGTACCAGTCAAGGTTTG+TGG + chr1_4:64120341-64120360 None:intergenic 45.0%
CACAAACCTTGACTGGTACA+AGG - chr1_4:64120339-64120358 Msa0177220:intron 45.0%
! GCCTCTTAGGCTCATTGATT+TGG + chr1_4:64120390-64120409 None:intergenic 45.0%
AGACATCCTGAAGTGGTAGT+CGG + chr1_4:64120429-64120448 None:intergenic 45.0%
CAGGATGTCTACAAGATTGG+AGG - chr1_4:64120439-64120458 Msa0177220:intron 45.0%
GCAAAAGTGACAACCATTCC+AGG + chr1_4:64120511-64120530 None:intergenic 45.0%
TTCAGTTGTCAGTCCAGTAG+GGG + chr1_4:64120533-64120552 None:intergenic 45.0%
CTTCAGTTGTCAGTCCAGTA+GGG + chr1_4:64120534-64120553 None:intergenic 45.0%
ACTTCAGTTGTCAGTCCAGT+AGG + chr1_4:64120535-64120554 None:intergenic 45.0%
GACAACTGAAGTCAAGTCTG+TGG - chr1_4:64120543-64120562 Msa0177220:intron 45.0%
CTGTTAAGGATCTCAAGCGT+GGG - chr1_4:64120635-64120654 Msa0177220:intron 45.0%
GTATGTTGCCTCAAACTCCA+AGG - chr1_4:64120657-64120676 Msa0177220:intron 45.0%
TGTAAAGTTAGCAGCCTCCT+TGG + chr1_4:64120692-64120711 None:intergenic 45.0%
AAACTTGACAGCAATGTGGG+AGG + chr1_4:64120785-64120804 None:intergenic 45.0%
CAGCAAACTTGACAGCAATG+TGG + chr1_4:64120789-64120808 None:intergenic 45.0%
GAGAAAGTCTCAACCACCAT+GGG + chr1_4:64120916-64120935 None:intergenic 45.0%
AGAGAAAGTCTCAACCACCA+TGG + chr1_4:64120917-64120936 None:intergenic 45.0%
ATGCGTCAAACTGTAGCTGT+TGG - chr1_4:64120973-64120992 Msa0177220:intron 45.0%
TGCGTCAAACTGTAGCTGTT+GGG - chr1_4:64120974-64120993 Msa0177220:intron 45.0%
GGTAGATAGATCATCCTGCT+CGG + chr1_4:64121076-64121095 None:intergenic 45.0%
AGCAGGATGATCTATCTACC+TGG - chr1_4:64121076-64121095 Msa0177220:intron 45.0%
GCAGGATGATCTATCTACCT+GGG - chr1_4:64121077-64121096 Msa0177220:intron 45.0%
!! AGAACTGGGTTCTAGATCGA+CGG - chr1_4:64121223-64121242 Msa0177220:intron 45.0%
GTAAGCAAGCAGTAAGTTCC+AGG - chr1_4:64121312-64121331 Msa0177220:five_prime_UTR 45.0%
! TTTAAGCTTTTGCTGGGGCA+TGG - chr1_4:64121397-64121416 Msa0177220:five_prime_UTR 45.0%
GAGAATAGAGAGGGACGGTA+GGG + chr1_4:64118268-64118287 None:intergenic 50.0%
TGAGAATAGAGAGGGACGGT+AGG + chr1_4:64118269-64118288 None:intergenic 50.0%
GAAACCCTAGTCGAGTAGCA+AGG + chr1_4:64118303-64118322 None:intergenic 50.0%
! GCCTTTTTCCCTTCTTCACG+AGG - chr1_4:64118332-64118351 Msa0177220:three_prime_UTR 50.0%
GGGTTGTTACCTCGTGAAGA+AGG + chr1_4:64118344-64118363 None:intergenic 50.0%
!!! GCTGGTTTTGAAGGAGTCGA+TGG - chr1_4:64118555-64118574 Msa0177220:three_prime_UTR 50.0%
TGTCCGGGACTTGACTCAAA+TGG - chr1_4:64119173-64119192 Msa0177220:CDS 50.0%
GCTCCATTTGAGTCAAGTCC+CGG + chr1_4:64119179-64119198 None:intergenic 50.0%
AATGGAGCCCTTTAACTCGG+TGG - chr1_4:64119191-64119210 Msa0177220:CDS 50.0%
GAAATGAATTGCCCTCACGC+AGG + chr1_4:64119393-64119412 None:intergenic 50.0%
!! TCATTGGCCATGTCGACTCT+GGG - chr1_4:64119662-64119681 Msa0177220:CDS 50.0%
TCTGGGAAATCAACCACCAC+TGG - chr1_4:64119679-64119698 Msa0177220:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG + chr1_4:64119695-64119714 None:intergenic 50.0%
TTGACAAGCGTGTCATCGAG+AGG - chr1_4:64119728-64119747 Msa0177220:CDS 50.0%
CTTAAGCTTGTCAAGGACCC+AGG + chr1_4:64119800-64119819 None:intergenic 50.0%
CTGGGTCCTTGACAAGCTTA+AGG - chr1_4:64119798-64119817 Msa0177220:CDS 50.0%
CTTAAGGCTGAACGGGAAAG+AGG - chr1_4:64119814-64119833 Msa0177220:CDS 50.0%
AATGAAATCCCTGTGTCCGG+GGG + chr1_4:64119905-64119924 None:intergenic 50.0%
GAGAACGGCACAATCAGCTT+GGG + chr1_4:64119950-64119969 None:intergenic 50.0%
TGAGAACGGCACAATCAGCT+TGG + chr1_4:64119951-64119970 None:intergenic 50.0%
GGCTTCAAAACCACCAGTAG+TGG + chr1_4:64119983-64120002 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG + chr1_4:64120028-64120047 None:intergenic 50.0%
GCTCTCCTTGCTTTCACTCT+TGG - chr1_4:64120036-64120055 Msa0177220:five_prime_UTR 50.0%
TTGGACCCTTGTACCAGTCA+AGG + chr1_4:64120348-64120367 None:intergenic 50.0%
TTGGTCAAGGGCCTCAAGAA+GGG + chr1_4:64120371-64120390 None:intergenic 50.0%
TTTGGTCAAGGGCCTCAAGA+AGG + chr1_4:64120372-64120391 None:intergenic 50.0%
ATCCTGAAGTGGTAGTCGGA+GGG + chr1_4:64120425-64120444 None:intergenic 50.0%
AGGAATTGGAACTGTGCCTG+TGG - chr1_4:64120459-64120478 Msa0177220:intron 50.0%
GGAATTGGAACTGTGCCTGT+GGG - chr1_4:64120460-64120479 Msa0177220:intron 50.0%
GAATTGGAACTGTGCCTGTG+GGG - chr1_4:64120461-64120480 Msa0177220:intron 50.0%
!!! GTTGTCACTTTTGCCCCTAC+TGG - chr1_4:64120517-64120536 Msa0177220:intron 50.0%
GCATCATGAAGCTCTCACAG+AGG - chr1_4:64120570-64120589 Msa0177220:intron 50.0%
TCCCACATTGTCACCAGGAA+GGG + chr1_4:64120596-64120615 None:intergenic 50.0%
ATCCCACATTGTCACCAGGA+AGG + chr1_4:64120597-64120616 None:intergenic 50.0%
GCTGTTAAGGATCTCAAGCG+TGG - chr1_4:64120634-64120653 Msa0177220:intron 50.0%
TGGGTCATCCTTGGAGTTTG+AGG + chr1_4:64120668-64120687 None:intergenic 50.0%
ATGAATCACCCTGGCCAGAT+TGG - chr1_4:64120727-64120746 Msa0177220:intron 50.0%
AGCCATTTCCAATCTGGCCA+GGG + chr1_4:64120738-64120757 None:intergenic 50.0%
TAGCCATTTCCAATCTGGCC+AGG + chr1_4:64120739-64120758 None:intergenic 50.0%
GGGCATAGCCATTTCCAATC+TGG + chr1_4:64120744-64120763 None:intergenic 50.0%
AGGTGTGGCAGTCAAGAACA+GGG + chr1_4:64120765-64120784 None:intergenic 50.0%
GATTGATAGGCGTTCCGGTA+AGG - chr1_4:64120822-64120841 Msa0177220:intron 50.0%
GCGTCAAACTGTAGCTGTTG+GGG - chr1_4:64120975-64120994 Msa0177220:intron 50.0%
TGTAGCTGTTGGGGTCATCA+AGG - chr1_4:64120984-64121003 Msa0177220:intron 50.0%
CATCAAGGCTGTGGAGAAGA+AGG - chr1_4:64120999-64121018 Msa0177220:intron 50.0%
GTCTGCTTAAGTCCTCCACT+GGG + chr1_4:64121027-64121046 None:intergenic 50.0%
GAAGAAGAAGTGAACCGAGC+AGG - chr1_4:64121059-64121078 Msa0177220:intron 50.0%
GAAACAGAGTCAGCAAGTCC+TGG + chr1_4:64121333-64121352 None:intergenic 50.0%
! TGCTGGGGCATGGTTCTATA+TGG - chr1_4:64121407-64121426 Msa0177220:five_prime_UTR 50.0%
AAGGGAAAAAGGCTGCGCAG+CGG + chr1_4:64118325-64118344 None:intergenic 55.0%
TCGGTGGTAGAGTAACACCG+TGG - chr1_4:64119207-64119226 Msa0177220:CDS 55.0%
CCTCACGCAGGATCGAACTA+CGG + chr1_4:64119381-64119400 None:intergenic 55.0%
CGTAGTTCGATCCTGCGTGA+GGG - chr1_4:64119379-64119398 Msa0177220:CDS 55.0%
!! GTCATTGGCCATGTCGACTC+TGG - chr1_4:64119661-64119680 Msa0177220:CDS 55.0%
TGCACTGTCATTGATGCCCC+CGG - chr1_4:64119886-64119905 Msa0177220:CDS 55.0%
AGCGCTCCACAAACCTTGAC+TGG - chr1_4:64120332-64120351 Msa0177220:intron 55.0%
CAAGGGTCCAACCCTTCTTG+AGG - chr1_4:64120357-64120376 Msa0177220:intron 55.0%
TCAAGGGCCTCAAGAAGGGT+TGG + chr1_4:64120367-64120386 None:intergenic 55.0%
GGCTTGTCTGAAGGCCTCTT+AGG + chr1_4:64120403-64120422 None:intergenic 55.0%
TCCTGAAGTGGTAGTCGGAG+GGG + chr1_4:64120424-64120443 None:intergenic 55.0%
CATCCTGAAGTGGTAGTCGG+AGG + chr1_4:64120426-64120445 None:intergenic 55.0%
GCCCTTCCTGGTGACAATGT+GGG - chr1_4:64120592-64120611 Msa0177220:intron 55.0%
AGTTAGCAGCCTCCTTGGCT+GGG + chr1_4:64120687-64120706 None:intergenic 55.0%
AAGTTAGCAGCCTCCTTGGC+TGG + chr1_4:64120688-64120707 None:intergenic 55.0%
CACCCTGGCCAGATTGGAAA+TGG - chr1_4:64120733-64120752 Msa0177220:intron 55.0%
GGTGTGGCAGTCAAGAACAG+GGG + chr1_4:64120764-64120783 None:intergenic 55.0%
GAGGTGTGGCAGTCAAGAAC+AGG + chr1_4:64120766-64120785 None:intergenic 55.0%
TGACAGCAATGTGGGAGGTG+TGG + chr1_4:64120780-64120799 None:intergenic 55.0%
GATGATTCCCACCAAGCCCA+TGG - chr1_4:64120897-64120916 Msa0177220:intron 55.0%
! TCAACCACCATGGGCTTGGT+GGG + chr1_4:64120907-64120926 None:intergenic 55.0%
AGTCTCAACCACCATGGGCT+TGG + chr1_4:64120911-64120930 None:intergenic 55.0%
CTAACAGCAAAACGGCCGAG+TGG + chr1_4:64120949-64120968 None:intergenic 55.0%
TGTTGGGGTCATCAAGGCTG+TGG - chr1_4:64120990-64121009 Msa0177220:intron 55.0%
GTGGAGAAGAAGGATCCCAG+TGG - chr1_4:64121009-64121028 Msa0177220:intron 55.0%
GAGAAGAAGGATCCCAGTGG+AGG - chr1_4:64121012-64121031 Msa0177220:intron 55.0%
GGTCTGCTTAAGTCCTCCAC+TGG + chr1_4:64121028-64121047 None:intergenic 55.0%
!! ACTGGGTTCTAGATCGACGG+TGG - chr1_4:64121226-64121245 Msa0177220:intron 55.0%
CCGTAGTTCGATCCTGCGTG+AGG - chr1_4:64119378-64119397 Msa0177220:CDS 60.0%
TCATTGATGCCCCCGGACAC+AGG - chr1_4:64119893-64119912 Msa0177220:CDS 60.0%
CATTGATGCCCCCGGACACA+GGG - chr1_4:64119894-64119913 Msa0177220:CDS 60.0%
AGTCGGAGGGGCTTGTCTGA+AGG + chr1_4:64120412-64120431 None:intergenic 60.0%
CCAGTTTCAACACGCCCCAC+AGG + chr1_4:64120478-64120497 None:intergenic 60.0%
CCTGTGGGGCGTGTTGAAAC+TGG - chr1_4:64120475-64120494 Msa0177220:intron 60.0%
GGCCCTTCCTGGTGACAATG+TGG - chr1_4:64120591-64120610 Msa0177220:intron 60.0%
CTCCAAGGATGACCCAGCCA+AGG - chr1_4:64120672-64120691 Msa0177220:intron 60.0%
CTCCTTGGCTGGGTCATCCT+TGG + chr1_4:64120677-64120696 None:intergenic 60.0%
CAAGGATGACCCAGCCAAGG+AGG - chr1_4:64120675-64120694 Msa0177220:intron 60.0%
GATTCCCACCAAGCCCATGG+TGG - chr1_4:64120900-64120919 Msa0177220:intron 60.0%
CTCAACCACCATGGGCTTGG+TGG + chr1_4:64120908-64120927 None:intergenic 60.0%
ACAGCAAAACGGCCGAGTGG+AGG + chr1_4:64120946-64120965 None:intergenic 60.0%
GCCCCTCCGACTACCACTTC+AGG - chr1_4:64120420-64120439 Msa0177220:intron 65.0%
GCTCTCACAGAGGCCCTTCC+TGG - chr1_4:64120580-64120599 Msa0177220:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 64118216 64121446 64118216 ID=Msa0177220;Name=Msa0177220
chr1_4 mRNA 64118216 64121446 64118216 ID=Msa0177220-mRNA-1;Parent=Msa0177220;Name=Msa0177220-mRNA-1;_AED=0.05;_eAED=0.05;_QI=170|1|1|1|0.5|0.33|3|375|448
chr1_4 exon 64118216 64119475 64118216 ID=Msa0177220-mRNA-1:exon:20840;Parent=Msa0177220-mRNA-1
chr1_4 exon 64119573 64120066 64119573 ID=Msa0177220-mRNA-1:exon:20839;Parent=Msa0177220-mRNA-1
chr1_4 exon 64121309 64121446 64121309 ID=Msa0177220-mRNA-1:exon:20838;Parent=Msa0177220-mRNA-1
chr1_4 five_prime_UTR 64121309 64121446 64121309 ID=Msa0177220-mRNA-1:five_prime_utr;Parent=Msa0177220-mRNA-1
chr1_4 five_prime_UTR 64120035 64120066 64120035 ID=Msa0177220-mRNA-1:five_prime_utr;Parent=Msa0177220-mRNA-1
chr1_4 CDS 64119573 64120034 64119573 ID=Msa0177220-mRNA-1:cds;Parent=Msa0177220-mRNA-1
chr1_4 CDS 64118591 64119475 64118591 ID=Msa0177220-mRNA-1:cds;Parent=Msa0177220-mRNA-1
chr1_4 three_prime_UTR 64118216 64118590 64118216 ID=Msa0177220-mRNA-1:three_prime_utr;Parent=Msa0177220-mRNA-1
Gene Sequence

>Msa0177220

ATGGGTAAGGAAAAGGTTCACATCAACATTGTGGTCATTGGCCATGTCGACTCTGGGAAATCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATCGAGAGGTTTGAGAAAGAAGCAGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTTAAGGCTGAACGGGAAAGAGGTATCACCATTGACATTGCTCTTTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCCGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGATTGTGCCGTTCTCATCATTGATTCCACTACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGATGGATGCTACTACACCCAAGTACTCCAAGGGTAGATATGATGAAATTGTGAAGGAAGTTTCATCCTATTTGAAGAAGGTTGGCTATAACCCAGACAAAATTCCATTTGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCCACAAACCTTGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCAAATCAATGAGCCTAAGAGGCCTTCAGACAAGCCCCTCCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTGCCTGTGGGGCGTGTTGAAACTGGTGTCATAAAACCTGGAATGGTTGTCACTTTTGCCCCTACTGGACTGACAACTGAAGTCAAGTCTGTGGAGATGCATCATGAAGCTCTCACAGAGGCCCTTCCTGGTGACAATGTGGGATTTAATGTCAAGAATGTTGCTGTTAAGGATCTCAAGCGTGGGTATGTTGCCTCAAACTCCAAGGATGACCCAGCCAAGGAGGCTGCTAACTTTACATCTCAAGTGATCATAATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTTCTTGACTGCCACACCTCCCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGATAGGCGTTCCGGTAAGGAAATTGAGAAGGAGCCCAAATTCTTGAAGAATGGTGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTGGTTGAGACTTTCTCTGAATATCCTCCACTCGGCCGTTTTGCTGTTAGAGACATGCGTCAAACTGTAGCTGTTGGGGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGTGGAGGACTTAAGCAGACCAAGTCAGCATTGAAGAAGAAGTGA
Protein sequence

>Msa0177220

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGLTTEVKSVEMHHEALTEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKAVEKKDPSGGLKQTKSALKKK*