AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1363390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363390 MtrunA17_Chr8g0387471 92.481 133 4 1 1 133 1 127 4.21e-85 244
Msa1363390 MtrunA17_Chr8g0348361 53.691 149 53 1 1 133 1 149 6.68e-37 123
Msa1363390 MtrunA17_Chr6g0469651 52.941 136 58 2 1 133 1 133 2.09e-33 114
Msa1363390 MtrunA17_Chr6g0469681 50.000 138 53 3 1 133 1 127 5.18e-33 112
Msa1363390 MtrunA17_Chr6g0469571 48.120 133 60 1 1 133 1 124 1.41e-32 111
Msa1363390 MtrunA17_Chr6g0469551 47.368 133 61 1 1 133 1 124 4.16e-32 110
Msa1363390 MtrunA17_Chr1g0202541 47.407 135 68 2 1 132 1 135 4.28e-32 110
Msa1363390 MtrunA17_Chr6g0469591 46.617 133 62 1 1 133 1 124 2.06e-30 105
Msa1363390 MtrunA17_Chr1g0202561 49.306 144 55 4 1 132 1 138 5.20e-29 102
Msa1363390 MtrunA17_Chr1g0202501 50.000 134 62 3 1 133 1 130 5.55e-29 102
Msa1363390 MtrunA17_Chr1g0202581 49.242 132 66 1 1 132 1 131 7.45e-29 102
Msa1363390 MtrunA17_Chr7g0248671 73.239 71 19 0 1 71 1 71 3.00e-28 102
Msa1363390 MtrunA17_Chr1g0204291 45.000 140 60 3 1 132 1 131 7.39e-28 99.8
Msa1363390 MtrunA17_Chr6g0469671 62.857 70 26 0 1 70 1 70 1.23e-27 98.6
Msa1363390 MtrunA17_Chr7g0233181 54.667 75 34 0 1 75 1 75 1.41e-24 90.5
Msa1363390 MtrunA17_Chr7g0233251 54.667 75 34 0 1 75 1 75 3.08e-24 89.7
Msa1363390 MtrunA17_Chr6g0469541 48.750 80 41 0 1 80 1 80 7.47e-24 89.0
Msa1363390 MtrunA17_Chr7g0233191 40.496 121 60 2 1 120 1 110 8.99e-24 88.6
Msa1363390 MtrunA17_Chr7g0233211 54.430 79 34 1 1 79 1 77 1.08e-23 88.6
Msa1363390 MtrunA17_Chr7g0233201 40.496 121 60 2 1 120 1 110 1.30e-23 88.2
Msa1363390 MtrunA17_Chr7g0233231 53.333 75 35 0 1 75 1 75 9.00e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363390 AT1G01490.1 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa1363390 AT1G01490.2 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa1363390 AT1G01490.4 48.571 175 46 3 3 133 30 204 1.41e-42 139
Msa1363390 AT1G01490.3 40.278 144 42 3 34 133 1 144 2.81e-21 83.6
Msa1363390 AT5G52740.1 43.609 133 56 4 1 131 1 116 6.51e-19 76.6
Msa1363390 AT5G52760.1 38.017 121 57 4 2 119 4 109 5.34e-16 69.7
Msa1363390 AT1G63950.2 44.776 67 37 0 1 67 1 67 1.76e-13 62.8
Msa1363390 AT1G63960.2 40.000 70 41 1 1 69 11 80 2.22e-12 60.5
Msa1363390 AT1G63950.1 42.188 64 37 0 4 67 1 64 1.18e-11 57.8
Msa1363390 AT5G52750.1 43.077 65 37 0 3 67 5 69 4.33e-11 57.0

Find 59 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCGGTTTCTAGCCTTTC+AGG 0.141123 tig0018036:+230041 Msa1363390:CDS
GGCACTTGTTTGACTTGTTT+TGG 0.244120 tig0018036:+229241 Msa1363390:five_prime_UTR
AGCTCAGCAATTTGTTCATT+TGG 0.261412 tig0018036:-230913 None:intergenic
TATGATAACACAAATTTAAA+TGG 0.263263 tig0018036:-231504 None:intergenic
CGTGGTCATTGGATTGAAAT+TGG 0.276908 tig0018036:+231123 Msa1363390:three_prime_UTR
TTGCGTGTAAATTATGAATT+AGG 0.295560 tig0018036:+231350 Msa1363390:three_prime_UTR
TTTCACACATTCACGCTATT+AGG 0.314334 tig0018036:+231094 Msa1363390:three_prime_UTR
ATCTAAATCCATAGTTGAAT+TGG 0.318872 tig0018036:+231281 Msa1363390:three_prime_UTR
AAAGCATAGAATAGAAGATT+TGG 0.328856 tig0018036:+231054 Msa1363390:three_prime_UTR
CAACAGATAACACAAGCATT+TGG 0.350285 tig0018036:-231006 None:intergenic
GGATTATGGTGTATTAAAAT+AGG 0.352846 tig0018036:+231415 Msa1363390:three_prime_UTR
TTGACTGATCTTCAAAACTT+TGG 0.364724 tig0018036:+229429 Msa1363390:five_prime_UTR
TGAGCTCACCAATTCAACTA+TGG 0.367558 tig0018036:-231289 None:intergenic
AAGTGTTGATATTTAATTGT+AGG 0.377594 tig0018036:-229408 None:intergenic
AATTTGCTCACAACATCAAC+AGG 0.390553 tig0018036:-230772 None:intergenic
AAGGCTAGAAACCGATTTCA+TGG 0.398452 tig0018036:-230036 None:intergenic
ATTCTATGCTTTCTCAATCA+AGG 0.399212 tig0018036:-231043 None:intergenic
CTTCTTGTCTTTCATGTCCA+TGG 0.402527 tig0018036:-230725 None:intergenic
AACAGATAACACAAGCATTT+GGG 0.403989 tig0018036:-231005 None:intergenic
TTAGGAGTGTCCGTGGTCAT+TGG 0.437252 tig0018036:+231112 Msa1363390:three_prime_UTR
AGACGTCCTGATGAGGATTA+TGG 0.438970 tig0018036:+231401 Msa1363390:three_prime_UTR
ATGATAGTATTGAGTCATGT+AGG 0.445771 tig0018036:-230962 None:intergenic
TGTATTAAAATAGGCTATGA+AGG 0.452511 tig0018036:+231424 Msa1363390:three_prime_UTR
GTGAGCTCAATGGAATTGAT+TGG 0.453628 tig0018036:+231303 Msa1363390:three_prime_UTR
GGAGAAGGAGCAGAGAAAGA+TGG 0.462159 tig0018036:-229326 None:intergenic
ATGGAATGCAACTTCGTGTT+GGG 0.465202 tig0018036:-229307 None:intergenic
TGAATGTTTAGAAGTGGAGA+AGG 0.466461 tig0018036:-229341 None:intergenic
TTTGTTCTTGTAGAAAATAG+TGG 0.476254 tig0018036:+229974 Msa1363390:intron
AATACACCATAATCCTCATC+AGG 0.477270 tig0018036:-231407 None:intergenic
GTAGAAAATAGTGGTGAAGT+TGG 0.484582 tig0018036:+229983 Msa1363390:intron
AAGGATTGAATGTTTAGAAG+TGG 0.485327 tig0018036:-229347 None:intergenic
ATGGCACAATGAACAAGTCC+TGG 0.485963 tig0018036:-231485 None:intergenic
TCAGAGGCGTCACTAATCAT+TGG 0.487006 tig0018036:-231225 None:intergenic
AAAGCAGAAAGCCATGAAAT+CGG 0.493020 tig0018036:+230025 Msa1363390:CDS
GTTGATATTTAATTGTAGGA+AGG 0.500952 tig0018036:-229404 None:intergenic
TGTTATCATACTTGTCAACT+TGG 0.503441 tig0018036:+231517 Msa1363390:three_prime_UTR
GATATTTAATTGTAGGAAGG+AGG 0.504732 tig0018036:-229401 None:intergenic
AGTTGAATTGGTGAGCTCAA+TGG 0.520253 tig0018036:+231293 Msa1363390:three_prime_UTR
AATTAAGAGAGGGAGTAGGA+AGG 0.531811 tig0018036:-229366 None:intergenic
GGTTGGGCCGGCAAAAGAGC+CGG 0.542911 tig0018036:+230825 Msa1363390:CDS
GACAAGAAGTTGACAGTGGT+GGG 0.556215 tig0018036:+230739 Msa1363390:CDS
GTGTTTCAATTCACACATCC+AGG 0.563797 tig0018036:+231467 Msa1363390:three_prime_UTR
TGATAGTATTGAGTCATGTA+GGG 0.568680 tig0018036:-230961 None:intergenic
TAGAAGATTTGGATTTAATG+CGG 0.573195 tig0018036:+231065 Msa1363390:three_prime_UTR
GATGGAATGCAACTTCGTGT+TGG 0.579616 tig0018036:-229308 None:intergenic
AGACAAGAAGTTGACAGTGG+TGG 0.596259 tig0018036:+230738 Msa1363390:CDS
TTGGACTCAAGTGAAAATGA+AGG 0.600404 tig0018036:+229448 Msa1363390:exon
CCTCTGACTACATGAATGTG+TGG 0.619190 tig0018036:+231241 Msa1363390:three_prime_UTR
AGGCATTGATTCTATCGCCA+TGG 0.631632 tig0018036:+230708 Msa1363390:intron
CCACACATTCATGTAGTCAG+AGG 0.634164 tig0018036:-231241 None:intergenic
TAGATTGAGACGTCCTGATG+AGG 0.634492 tig0018036:+231394 Msa1363390:three_prime_UTR
CACGCTATTAGGAGTGTCCG+TGG 0.642475 tig0018036:+231105 Msa1363390:three_prime_UTR
ATCATTGGATTGAGATCACA+CGG 0.642508 tig0018036:-231210 None:intergenic
GTGACCAGTGAGTACCTGAA+AGG 0.659865 tig0018036:-230055 None:intergenic
GAGCTTATTAAGCAATACAG+AGG 0.663132 tig0018036:+230931 Msa1363390:CDS
CAATTTCAATCCAATGACCA+CGG 0.698408 tig0018036:-231122 None:intergenic
ATTTGCTCACAACATCAACA+GGG 0.737533 tig0018036:-230771 None:intergenic
GAAAGACAAGAAGTTGACAG+TGG 0.740845 tig0018036:+230735 Msa1363390:CDS
AATGTGTGGATTGTTAATTG+CGG 0.752410 tig0018036:+231255 Msa1363390:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATTTATTAATTATT+TGG + tig0018036:230353-230372 Msa1363390:intron 0.0%
!! ATATTTATTTATTAATTATT+TGG + tig0018036:230353-230372 Msa1363390:intron 0.0%
!! ATTAATTAAACTATTATAGT+TGG + tig0018036:229722-229741 Msa1363390:intron 10.0%
!! ATTTACTATTACTAAATTAA+TGG - tig0018036:230106-230125 None:intergenic 10.0%
!! ATTAATTAAACTATTATAGT+TGG + tig0018036:229722-229741 Msa1363390:intron 10.0%
!! ATTTACTATTACTAAATTAA+TGG - tig0018036:230106-230125 None:intergenic 10.0%
!! ATTTCATATCAATTCAAATA+AGG + tig0018036:229585-229604 Msa1363390:intron 15.0%
!! TTTAATTAATGAAACAAGTA+AGG - tig0018036:229712-229731 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG + tig0018036:229943-229962 Msa1363390:intron 15.0%
!! ATTAATAAATAAATATCTGC+TGG - tig0018036:230349-230368 None:intergenic 15.0%
!!! ATGTATTTTTACACATAAAT+GGG - tig0018036:230599-230618 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG - tig0018036:230632-230651 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG - tig0018036:230686-230705 None:intergenic 15.0%
!! TATGATAACACAAATTTAAA+TGG - tig0018036:231507-231526 None:intergenic 15.0%
!! ATTTCATATCAATTCAAATA+AGG + tig0018036:229585-229604 Msa1363390:intron 15.0%
!! TTTAATTAATGAAACAAGTA+AGG - tig0018036:229712-229731 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG + tig0018036:229943-229962 Msa1363390:intron 15.0%
!! ATTAATAAATAAATATCTGC+TGG - tig0018036:230349-230368 None:intergenic 15.0%
!!! ATGTATTTTTACACATAAAT+GGG - tig0018036:230599-230618 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG - tig0018036:230632-230651 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG - tig0018036:230686-230705 None:intergenic 15.0%
!! TATGATAACACAAATTTAAA+TGG - tig0018036:231507-231526 None:intergenic 15.0%
!! TGAATAGAAAAATTAAGAGA+GGG - tig0018036:229379-229398 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG - tig0018036:229411-229430 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG - tig0018036:229570-229589 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG - tig0018036:229615-229634 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG - tig0018036:230099-230118 None:intergenic 20.0%
!! TTAATGGAATTTGTACTAAA+AGG - tig0018036:230137-230156 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG - tig0018036:230153-230172 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG + tig0018036:230319-230338 Msa1363390:intron 20.0%
!!! GATGTATTTTTACACATAAA+TGG - tig0018036:230600-230619 None:intergenic 20.0%
!! GAAAAATTAAACAATAAGCT+TGG + tig0018036:231179-231198 Msa1363390:three_prime_UTR 20.0%
!! TGAATAGAAAAATTAAGAGA+GGG - tig0018036:229379-229398 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG - tig0018036:229411-229430 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG - tig0018036:229570-229589 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG - tig0018036:229615-229634 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG - tig0018036:230099-230118 None:intergenic 20.0%
!! TTAATGGAATTTGTACTAAA+AGG - tig0018036:230137-230156 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG - tig0018036:230153-230172 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG + tig0018036:230319-230338 Msa1363390:intron 20.0%
!!! GATGTATTTTTACACATAAA+TGG - tig0018036:230600-230619 None:intergenic 20.0%
!! GAAAAATTAAACAATAAGCT+TGG + tig0018036:231179-231198 Msa1363390:three_prime_UTR 20.0%
! GTGAATAGAAAAATTAAGAG+AGG - tig0018036:229380-229399 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG - tig0018036:229407-229426 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG + tig0018036:229474-229493 Msa1363390:intron 25.0%
! TCAAAGAAAAAAACAGAACA+TGG - tig0018036:229526-229545 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG - tig0018036:229560-229579 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG - tig0018036:229647-229666 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG - tig0018036:229650-229669 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG - tig0018036:229705-229724 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG - tig0018036:229706-229725 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG - tig0018036:229898-229917 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG + tig0018036:229974-229993 Msa1363390:intron 25.0%
! CCTTTGTTAATTAAAATGGA+CGG + tig0018036:230323-230342 Msa1363390:intron 25.0%
!!! ATATGATTTTTTGCTTGCTT+TGG - tig0018036:230540-230559 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG + tig0018036:230688-230707 Msa1363390:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG + tig0018036:231054-231073 Msa1363390:three_prime_UTR 25.0%
! TAGAAGATTTGGATTTAATG+CGG + tig0018036:231065-231084 Msa1363390:three_prime_UTR 25.0%
! ATCTAAATCCATAGTTGAAT+TGG + tig0018036:231281-231300 Msa1363390:three_prime_UTR 25.0%
! TTGCGTGTAAATTATGAATT+AGG + tig0018036:231350-231369 Msa1363390:three_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG + tig0018036:231415-231434 Msa1363390:three_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG + tig0018036:231424-231443 Msa1363390:three_prime_UTR 25.0%
! GTGAATAGAAAAATTAAGAG+AGG - tig0018036:229380-229399 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG - tig0018036:229407-229426 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG + tig0018036:229474-229493 Msa1363390:intron 25.0%
! TCAAAGAAAAAAACAGAACA+TGG - tig0018036:229526-229545 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG - tig0018036:229560-229579 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG - tig0018036:229647-229666 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG - tig0018036:229650-229669 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG - tig0018036:229705-229724 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG - tig0018036:229706-229725 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG - tig0018036:229898-229917 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG + tig0018036:229974-229993 Msa1363390:intron 25.0%
! CCTTTGTTAATTAAAATGGA+CGG + tig0018036:230323-230342 Msa1363390:intron 25.0%
!!! ATATGATTTTTTGCTTGCTT+TGG - tig0018036:230540-230559 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG + tig0018036:230688-230707 Msa1363390:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG + tig0018036:231054-231073 Msa1363390:three_prime_UTR 25.0%
! TAGAAGATTTGGATTTAATG+CGG + tig0018036:231065-231084 Msa1363390:three_prime_UTR 25.0%
! ATCTAAATCCATAGTTGAAT+TGG + tig0018036:231281-231300 Msa1363390:three_prime_UTR 25.0%
! TTGCGTGTAAATTATGAATT+AGG + tig0018036:231350-231369 Msa1363390:three_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG + tig0018036:231415-231434 Msa1363390:three_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG + tig0018036:231424-231443 Msa1363390:three_prime_UTR 25.0%
AAGGATTGAATGTTTAGAAG+TGG - tig0018036:229350-229369 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG - tig0018036:229404-229423 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG + tig0018036:229429-229448 Msa1363390:five_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG - tig0018036:229704-229723 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG + tig0018036:229818-229837 Msa1363390:intron 30.0%
CAGGAAAATGAAATCATTGT+TGG - tig0018036:230253-230272 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG + tig0018036:230267-230286 Msa1363390:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG - tig0018036:230326-230345 None:intergenic 30.0%
ATTCAAAAAACATCACTCCA+TGG - tig0018036:230397-230416 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG + tig0018036:230506-230525 Msa1363390:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG + tig0018036:230640-230659 Msa1363390:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG + tig0018036:230785-230804 Msa1363390:CDS 30.0%
! TTGGCACACAGAAATTTTAA+CGG + tig0018036:230804-230823 Msa1363390:CDS 30.0%
!! ACACAGAAATTTTAACGGTT+GGG + tig0018036:230809-230828 Msa1363390:CDS 30.0%
! TGATAGTATTGAGTCATGTA+GGG - tig0018036:230964-230983 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG - tig0018036:230965-230984 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG - tig0018036:231008-231027 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG - tig0018036:231046-231065 None:intergenic 30.0%
! AATGTGTGGATTGTTAATTG+CGG + tig0018036:231255-231274 Msa1363390:three_prime_UTR 30.0%
TTGGACATGAACAAAAAGTA+AGG + tig0018036:231322-231341 Msa1363390:three_prime_UTR 30.0%
TGTTATCATACTTGTCAACT+TGG + tig0018036:231517-231536 Msa1363390:three_prime_UTR 30.0%
AAGGATTGAATGTTTAGAAG+TGG - tig0018036:229350-229369 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG - tig0018036:229404-229423 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG + tig0018036:229429-229448 Msa1363390:five_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG - tig0018036:229704-229723 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG + tig0018036:229818-229837 Msa1363390:intron 30.0%
CAGGAAAATGAAATCATTGT+TGG - tig0018036:230253-230272 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG + tig0018036:230267-230286 Msa1363390:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG - tig0018036:230326-230345 None:intergenic 30.0%
ATTCAAAAAACATCACTCCA+TGG - tig0018036:230397-230416 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG + tig0018036:230506-230525 Msa1363390:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG + tig0018036:230640-230659 Msa1363390:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG + tig0018036:230785-230804 Msa1363390:CDS 30.0%
! TTGGCACACAGAAATTTTAA+CGG + tig0018036:230804-230823 Msa1363390:CDS 30.0%
!! ACACAGAAATTTTAACGGTT+GGG + tig0018036:230809-230828 Msa1363390:CDS 30.0%
! TGATAGTATTGAGTCATGTA+GGG - tig0018036:230964-230983 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG - tig0018036:230965-230984 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG - tig0018036:231008-231027 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG - tig0018036:231046-231065 None:intergenic 30.0%
! AATGTGTGGATTGTTAATTG+CGG + tig0018036:231255-231274 Msa1363390:three_prime_UTR 30.0%
TTGGACATGAACAAAAAGTA+AGG + tig0018036:231322-231341 Msa1363390:three_prime_UTR 30.0%
TGTTATCATACTTGTCAACT+TGG + tig0018036:231517-231536 Msa1363390:three_prime_UTR 30.0%
TGAATGTTTAGAAGTGGAGA+AGG - tig0018036:229344-229363 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG - tig0018036:229373-229392 None:intergenic 35.0%
TTGGACTCAAGTGAAAATGA+AGG + tig0018036:229448-229467 Msa1363390:exon 35.0%
GTAGAAAATAGTGGTGAAGT+TGG + tig0018036:229983-230002 Msa1363390:intron 35.0%
AAAGCAGAAAGCCATGAAAT+CGG + tig0018036:230025-230044 Msa1363390:CDS 35.0%
GGAATTTGTACTAAAAGGAC+AGG - tig0018036:230132-230151 None:intergenic 35.0%
GTCCAATTGTATACTTACTC+AGG - tig0018036:230272-230291 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG + tig0018036:230377-230396 Msa1363390:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG + tig0018036:230457-230476 Msa1363390:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG + tig0018036:230458-230477 Msa1363390:intron 35.0%
ACACATTGTCATGTATGAGT+CGG - tig0018036:230502-230521 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG + tig0018036:230511-230530 Msa1363390:intron 35.0%
ATTTGCTCACAACATCAACA+GGG - tig0018036:230774-230793 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG - tig0018036:230775-230794 None:intergenic 35.0%
!! CACACAGAAATTTTAACGGT+TGG + tig0018036:230808-230827 Msa1363390:CDS 35.0%
! AGCTCAGCAATTTGTTCATT+TGG - tig0018036:230916-230935 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG + tig0018036:230931-230950 Msa1363390:CDS 35.0%
CAACAGATAACACAAGCATT+TGG - tig0018036:231009-231028 None:intergenic 35.0%
TTTCACACATTCACGCTATT+AGG + tig0018036:231094-231113 Msa1363390:three_prime_UTR 35.0%
CAATTTCAATCCAATGACCA+CGG - tig0018036:231125-231144 None:intergenic 35.0%
ATCATTGGATTGAGATCACA+CGG - tig0018036:231213-231232 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG - tig0018036:231410-231429 None:intergenic 35.0%
TGAATGTTTAGAAGTGGAGA+AGG - tig0018036:229344-229363 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG - tig0018036:229373-229392 None:intergenic 35.0%
TTGGACTCAAGTGAAAATGA+AGG + tig0018036:229448-229467 Msa1363390:exon 35.0%
GTAGAAAATAGTGGTGAAGT+TGG + tig0018036:229983-230002 Msa1363390:intron 35.0%
AAAGCAGAAAGCCATGAAAT+CGG + tig0018036:230025-230044 Msa1363390:CDS 35.0%
GGAATTTGTACTAAAAGGAC+AGG - tig0018036:230132-230151 None:intergenic 35.0%
GTCCAATTGTATACTTACTC+AGG - tig0018036:230272-230291 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG + tig0018036:230377-230396 Msa1363390:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG + tig0018036:230457-230476 Msa1363390:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG + tig0018036:230458-230477 Msa1363390:intron 35.0%
ACACATTGTCATGTATGAGT+CGG - tig0018036:230502-230521 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG + tig0018036:230511-230530 Msa1363390:intron 35.0%
ATTTGCTCACAACATCAACA+GGG - tig0018036:230774-230793 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG - tig0018036:230775-230794 None:intergenic 35.0%
!! CACACAGAAATTTTAACGGT+TGG + tig0018036:230808-230827 Msa1363390:CDS 35.0%
! AGCTCAGCAATTTGTTCATT+TGG - tig0018036:230916-230935 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG + tig0018036:230931-230950 Msa1363390:CDS 35.0%
CAACAGATAACACAAGCATT+TGG - tig0018036:231009-231028 None:intergenic 35.0%
TTTCACACATTCACGCTATT+AGG + tig0018036:231094-231113 Msa1363390:three_prime_UTR 35.0%
CAATTTCAATCCAATGACCA+CGG - tig0018036:231125-231144 None:intergenic 35.0%
ATCATTGGATTGAGATCACA+CGG - tig0018036:231213-231232 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG - tig0018036:231410-231429 None:intergenic 35.0%
!!! GGCACTTGTTTGACTTGTTT+TGG + tig0018036:229241-229260 Msa1363390:five_prime_UTR 40.0%
ATGGAATGCAACTTCGTGTT+GGG - tig0018036:229310-229329 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG - tig0018036:229369-229388 None:intergenic 40.0%
! AAGGCTAGAAACCGATTTCA+TGG - tig0018036:230039-230058 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG + tig0018036:230041-230060 Msa1363390:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG + tig0018036:230459-230478 Msa1363390:intron 40.0%
ACATAAATGGGACAACTGCA+AGG - tig0018036:230587-230606 None:intergenic 40.0%
! TGTCCGATTGAGACATGTTT+TGG - tig0018036:230646-230665 None:intergenic 40.0%
CTTCTTGTCTTTCATGTCCA+TGG - tig0018036:230728-230747 None:intergenic 40.0%
GAAAGACAAGAAGTTGACAG+TGG + tig0018036:230735-230754 Msa1363390:CDS 40.0%
!! AGAAATTTTAACGGTTGGGC+CGG + tig0018036:230813-230832 Msa1363390:CDS 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG - tig0018036:230847-230866 None:intergenic 40.0%
CGTGGTCATTGGATTGAAAT+TGG + tig0018036:231123-231142 Msa1363390:three_prime_UTR 40.0%
TGAGCTCACCAATTCAACTA+TGG - tig0018036:231292-231311 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG + tig0018036:231293-231312 Msa1363390:three_prime_UTR 40.0%
GTGAGCTCAATGGAATTGAT+TGG + tig0018036:231303-231322 Msa1363390:three_prime_UTR 40.0%
GTGTTTCAATTCACACATCC+AGG + tig0018036:231467-231486 Msa1363390:three_prime_UTR 40.0%
!!! GGCACTTGTTTGACTTGTTT+TGG + tig0018036:229241-229260 Msa1363390:five_prime_UTR 40.0%
ATGGAATGCAACTTCGTGTT+GGG - tig0018036:229310-229329 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG - tig0018036:229369-229388 None:intergenic 40.0%
! AAGGCTAGAAACCGATTTCA+TGG - tig0018036:230039-230058 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG + tig0018036:230041-230060 Msa1363390:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG + tig0018036:230459-230478 Msa1363390:intron 40.0%
ACATAAATGGGACAACTGCA+AGG - tig0018036:230587-230606 None:intergenic 40.0%
! TGTCCGATTGAGACATGTTT+TGG - tig0018036:230646-230665 None:intergenic 40.0%
CTTCTTGTCTTTCATGTCCA+TGG - tig0018036:230728-230747 None:intergenic 40.0%
GAAAGACAAGAAGTTGACAG+TGG + tig0018036:230735-230754 Msa1363390:CDS 40.0%
!! AGAAATTTTAACGGTTGGGC+CGG + tig0018036:230813-230832 Msa1363390:CDS 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG - tig0018036:230847-230866 None:intergenic 40.0%
CGTGGTCATTGGATTGAAAT+TGG + tig0018036:231123-231142 Msa1363390:three_prime_UTR 40.0%
TGAGCTCACCAATTCAACTA+TGG - tig0018036:231292-231311 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG + tig0018036:231293-231312 Msa1363390:three_prime_UTR 40.0%
GTGAGCTCAATGGAATTGAT+TGG + tig0018036:231303-231322 Msa1363390:three_prime_UTR 40.0%
GTGTTTCAATTCACACATCC+AGG + tig0018036:231467-231486 Msa1363390:three_prime_UTR 40.0%
GATGGAATGCAACTTCGTGT+TGG - tig0018036:229311-229330 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG - tig0018036:229507-229526 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG + tig0018036:230068-230087 Msa1363390:intron 45.0%
ACAGTTGTCCCCAAAACGAA+CGG - tig0018036:230470-230489 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG + tig0018036:230708-230727 Msa1363390:intron 45.0%
AGACAAGAAGTTGACAGTGG+TGG + tig0018036:230738-230757 Msa1363390:CDS 45.0%
GACAAGAAGTTGACAGTGGT+GGG + tig0018036:230739-230758 Msa1363390:CDS 45.0%
CCGGAGAAGAAAAAAGACGA+AGG + tig0018036:230844-230863 Msa1363390:CDS 45.0%
!! TCAGAGGCGTCACTAATCAT+TGG - tig0018036:231228-231247 None:intergenic 45.0%
CCACACATTCATGTAGTCAG+AGG - tig0018036:231244-231263 None:intergenic 45.0%
CCTCTGACTACATGAATGTG+TGG + tig0018036:231241-231260 Msa1363390:three_prime_UTR 45.0%
TAGATTGAGACGTCCTGATG+AGG + tig0018036:231394-231413 Msa1363390:three_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG + tig0018036:231401-231420 Msa1363390:three_prime_UTR 45.0%
ATGGCACAATGAACAAGTCC+TGG - tig0018036:231488-231507 None:intergenic 45.0%
GATGGAATGCAACTTCGTGT+TGG - tig0018036:229311-229330 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG - tig0018036:229507-229526 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG + tig0018036:230068-230087 Msa1363390:intron 45.0%
ACAGTTGTCCCCAAAACGAA+CGG - tig0018036:230470-230489 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG + tig0018036:230708-230727 Msa1363390:intron 45.0%
AGACAAGAAGTTGACAGTGG+TGG + tig0018036:230738-230757 Msa1363390:CDS 45.0%
GACAAGAAGTTGACAGTGGT+GGG + tig0018036:230739-230758 Msa1363390:CDS 45.0%
CCGGAGAAGAAAAAAGACGA+AGG + tig0018036:230844-230863 Msa1363390:CDS 45.0%
!! TCAGAGGCGTCACTAATCAT+TGG - tig0018036:231228-231247 None:intergenic 45.0%
CCACACATTCATGTAGTCAG+AGG - tig0018036:231244-231263 None:intergenic 45.0%
CCTCTGACTACATGAATGTG+TGG + tig0018036:231241-231260 Msa1363390:three_prime_UTR 45.0%
TAGATTGAGACGTCCTGATG+AGG + tig0018036:231394-231413 Msa1363390:three_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG + tig0018036:231401-231420 Msa1363390:three_prime_UTR 45.0%
ATGGCACAATGAACAAGTCC+TGG - tig0018036:231488-231507 None:intergenic 45.0%
!! ATATCAAAAATAAATAATAA+AGG + tig0018036:230556-230575 Msa1363390:intron 5.0%
!! ATATCAAAAATAAATAATAA+AGG + tig0018036:230556-230575 Msa1363390:intron 5.0%
GGAGAAGGAGCAGAGAAAGA+TGG - tig0018036:229329-229348 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG + tig0018036:230051-230070 Msa1363390:intron 50.0%
GTGACCAGTGAGTACCTGAA+AGG - tig0018036:230058-230077 None:intergenic 50.0%
AAATGGGACAACTGCAAGGC+TGG - tig0018036:230583-230602 None:intergenic 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG - tig0018036:230835-230854 None:intergenic 50.0%
!! TTAGGAGTGTCCGTGGTCAT+TGG + tig0018036:231112-231131 Msa1363390:three_prime_UTR 50.0%
GGAGAAGGAGCAGAGAAAGA+TGG - tig0018036:229329-229348 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG + tig0018036:230051-230070 Msa1363390:intron 50.0%
GTGACCAGTGAGTACCTGAA+AGG - tig0018036:230058-230077 None:intergenic 50.0%
AAATGGGACAACTGCAAGGC+TGG - tig0018036:230583-230602 None:intergenic 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG - tig0018036:230835-230854 None:intergenic 50.0%
!! TTAGGAGTGTCCGTGGTCAT+TGG + tig0018036:231112-231131 Msa1363390:three_prime_UTR 50.0%
!! CACGCTATTAGGAGTGTCCG+TGG + tig0018036:231105-231124 Msa1363390:three_prime_UTR 55.0%
!! CACGCTATTAGGAGTGTCCG+TGG + tig0018036:231105-231124 Msa1363390:three_prime_UTR 55.0%
GGTTGGGCCGGCAAAAGAGC+CGG + tig0018036:230825-230844 Msa1363390:CDS 65.0%
GGTTGGGCCGGCAAAAGAGC+CGG + tig0018036:230825-230844 Msa1363390:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018036 gene 229237 231575 229237 ID=Msa1363390;Name=Msa1363390
tig0018036 mRNA 229237 231575 229237 ID=Msa1363390-mRNA-1;Parent=Msa1363390;Name=Msa1363390-mRNA-1;_AED=0.07;_eAED=0.07;_QI=227|1|1|1|1|1|3|546|133
tig0018036 exon 229237 229469 229237 ID=Msa1363390-mRNA-1:exon:13476;Parent=Msa1363390-mRNA-1
tig0018036 exon 229987 230062 229987 ID=Msa1363390-mRNA-1:exon:13477;Parent=Msa1363390-mRNA-1
tig0018036 exon 230710 231575 230710 ID=Msa1363390-mRNA-1:exon:13478;Parent=Msa1363390-mRNA-1
tig0018036 five_prime_UTR 229237 229463 229237 ID=Msa1363390-mRNA-1:five_prime_utr;Parent=Msa1363390-mRNA-1
tig0018036 CDS 229464 229469 229464 ID=Msa1363390-mRNA-1:cds;Parent=Msa1363390-mRNA-1
tig0018036 CDS 229987 230062 229987 ID=Msa1363390-mRNA-1:cds;Parent=Msa1363390-mRNA-1
tig0018036 CDS 230710 231029 230710 ID=Msa1363390-mRNA-1:cds;Parent=Msa1363390-mRNA-1
tig0018036 three_prime_UTR 231030 231575 231030 ID=Msa1363390-mRNA-1:three_prime_utr;Parent=Msa1363390-mRNA-1
Gene Sequence

>Msa1363390

ATGAAGAAAATAGTGGTGAAGTTGGACTTGCATGATGACAAAGCAAAGCAGAAAGCCATGAAATCGGTTTCTAGCCTTTCAGGCATTGATTCTATCGCCATGGACATGAAAGACAAGAAGTTGACAGTGGTGGGCAACATAGACCCTGTTGATGTTGTGAGCAAATTGAGAAAAACTTGGCACACAGAAATTTTAACGGTTGGGCCGGCAAAAGAGCCGGAGAAGAAAAAAGACGAAGGCAAAAAAGATGATAATAAGAAGAAAGAAGATGACAAGAAGAAAGATCCAAATGAACAAATTGCTGAGCTTATTAAGCAATACAGAGGATACAATCCCTACATGACTCAATACTATCATGTTCAGAGTGTAGAAGAGAACCCAAATGCTTGTGTTATCTGTTGA
Protein sequence

>Msa1363390

MKKIVVKLDLHDDKAKQKAMKSVSSLSGIDSIAMDMKDKKLTVVGNIDPVDVVSKLRKTWHTEILTVGPAKEPEKKKDEGKKDDNKKKEDDKKKDPNEQIAELIKQYRGYNPYMTQYYHVQSVEENPNACVIC*