AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0306610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0306610 MtrunA17_Chr8g0387471 92.481 133 4 1 1 133 1 127 4.21e-85 244
Msa0306610 MtrunA17_Chr8g0348361 53.691 149 53 1 1 133 1 149 6.68e-37 123
Msa0306610 MtrunA17_Chr6g0469651 52.941 136 58 2 1 133 1 133 2.09e-33 114
Msa0306610 MtrunA17_Chr6g0469681 50.000 138 53 3 1 133 1 127 5.18e-33 112
Msa0306610 MtrunA17_Chr6g0469571 48.120 133 60 1 1 133 1 124 1.41e-32 111
Msa0306610 MtrunA17_Chr6g0469551 47.368 133 61 1 1 133 1 124 4.16e-32 110
Msa0306610 MtrunA17_Chr1g0202541 47.407 135 68 2 1 132 1 135 4.28e-32 110
Msa0306610 MtrunA17_Chr6g0469591 46.617 133 62 1 1 133 1 124 2.06e-30 105
Msa0306610 MtrunA17_Chr1g0202561 49.306 144 55 4 1 132 1 138 5.20e-29 102
Msa0306610 MtrunA17_Chr1g0202501 50.000 134 62 3 1 133 1 130 5.55e-29 102
Msa0306610 MtrunA17_Chr1g0202581 49.242 132 66 1 1 132 1 131 7.45e-29 102
Msa0306610 MtrunA17_Chr7g0248671 73.239 71 19 0 1 71 1 71 3.00e-28 102
Msa0306610 MtrunA17_Chr1g0204291 45.000 140 60 3 1 132 1 131 7.39e-28 99.8
Msa0306610 MtrunA17_Chr6g0469671 62.857 70 26 0 1 70 1 70 1.23e-27 98.6
Msa0306610 MtrunA17_Chr7g0233181 54.667 75 34 0 1 75 1 75 1.41e-24 90.5
Msa0306610 MtrunA17_Chr7g0233251 54.667 75 34 0 1 75 1 75 3.08e-24 89.7
Msa0306610 MtrunA17_Chr6g0469541 48.750 80 41 0 1 80 1 80 7.47e-24 89.0
Msa0306610 MtrunA17_Chr7g0233191 40.496 121 60 2 1 120 1 110 8.99e-24 88.6
Msa0306610 MtrunA17_Chr7g0233211 54.430 79 34 1 1 79 1 77 1.08e-23 88.6
Msa0306610 MtrunA17_Chr7g0233201 40.496 121 60 2 1 120 1 110 1.30e-23 88.2
Msa0306610 MtrunA17_Chr7g0233231 53.333 75 35 0 1 75 1 75 9.00e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0306610 AT1G01490.1 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa0306610 AT1G01490.2 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa0306610 AT1G01490.4 48.571 175 46 3 3 133 30 204 1.41e-42 139
Msa0306610 AT1G01490.3 40.278 144 42 3 34 133 1 144 2.81e-21 83.6
Msa0306610 AT5G52740.1 43.609 133 56 4 1 131 1 116 6.51e-19 76.6
Msa0306610 AT5G52760.1 38.017 121 57 4 2 119 4 109 5.34e-16 69.7
Msa0306610 AT1G63950.2 44.776 67 37 0 1 67 1 67 1.76e-13 62.8
Msa0306610 AT1G63960.2 40.000 70 41 1 1 69 11 80 2.22e-12 60.5
Msa0306610 AT1G63950.1 42.188 64 37 0 4 67 1 64 1.18e-11 57.8
Msa0306610 AT5G52750.1 43.077 65 37 0 3 67 5 69 4.33e-11 57.0

Find 59 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCGGTTTCTAGCCTTTC+AGG 0.141123 2_3:-65641820 Msa0306610:intron
GGCACTTGTTTGACTTGTTT+TGG 0.244120 2_3:-65642619 Msa0306610:five_prime_UTR
AGCTCAGCAATTTGTTCATT+TGG 0.261412 2_3:+65640948 None:intergenic
TATGATAACACAAATTTAAA+TGG 0.263263 2_3:+65640357 None:intergenic
CGTGGTCATTGGATTGAAAT+TGG 0.276908 2_3:-65640738 Msa0306610:three_prime_UTR
TTGCGTGTAAATTATGAATT+AGG 0.295560 2_3:-65640511 Msa0306610:three_prime_UTR
TTTCACACATTCACGCTATT+AGG 0.314334 2_3:-65640767 Msa0306610:three_prime_UTR
ATCTAAATCCATAGTTGAAT+TGG 0.318872 2_3:-65640580 Msa0306610:three_prime_UTR
AAAGCATAGAATAGAAGATT+TGG 0.328856 2_3:-65640807 Msa0306610:three_prime_UTR
CAACAGATAACACAAGCATT+TGG 0.350285 2_3:+65640855 None:intergenic
GGATTATGGTGTATTAAAAT+AGG 0.352846 2_3:-65640446 Msa0306610:three_prime_UTR
TTGACTGATCTTCAAAACTT+TGG 0.364724 2_3:-65642431 Msa0306610:five_prime_UTR
TGAGCTCACCAATTCAACTA+TGG 0.367558 2_3:+65640572 None:intergenic
AAGTGTTGATATTTAATTGT+AGG 0.377594 2_3:+65642452 None:intergenic
AATTTGCTCACAACATCAAC+AGG 0.390553 2_3:+65641089 None:intergenic
AAGGCTAGAAACCGATTTCA+TGG 0.398452 2_3:+65641825 None:intergenic
ATTCTATGCTTTCTCAATCA+AGG 0.399212 2_3:+65640818 None:intergenic
CTTCTTGTCTTTCATGTCCA+TGG 0.402527 2_3:+65641136 None:intergenic
AACAGATAACACAAGCATTT+GGG 0.403989 2_3:+65640856 None:intergenic
TTAGGAGTGTCCGTGGTCAT+TGG 0.437252 2_3:-65640749 Msa0306610:three_prime_UTR
AGACGTCCTGATGAGGATTA+TGG 0.438970 2_3:-65640460 Msa0306610:three_prime_UTR
ATGATAGTATTGAGTCATGT+AGG 0.445771 2_3:+65640899 None:intergenic
TGTATTAAAATAGGCTATGA+AGG 0.452511 2_3:-65640437 Msa0306610:three_prime_UTR
GTGAGCTCAATGGAATTGAT+TGG 0.453628 2_3:-65640558 Msa0306610:three_prime_UTR
GGAGAAGGAGCAGAGAAAGA+TGG 0.462159 2_3:+65642534 None:intergenic
ATGGAATGCAACTTCGTGTT+GGG 0.465202 2_3:+65642553 None:intergenic
TGAATGTTTAGAAGTGGAGA+AGG 0.466461 2_3:+65642519 None:intergenic
TTTGTTCTTGTAGAAAATAG+TGG 0.476254 2_3:-65641887 Msa0306610:intron
AATACACCATAATCCTCATC+AGG 0.477270 2_3:+65640454 None:intergenic
GTAGAAAATAGTGGTGAAGT+TGG 0.484582 2_3:-65641878 Msa0306610:intron
AAGGATTGAATGTTTAGAAG+TGG 0.485327 2_3:+65642513 None:intergenic
ATGGCACAATGAACAAGTCC+TGG 0.485963 2_3:+65640376 None:intergenic
TCAGAGGCGTCACTAATCAT+TGG 0.487006 2_3:+65640636 None:intergenic
AAAGCAGAAAGCCATGAAAT+CGG 0.493020 2_3:-65641836 Msa0306610:CDS
GTTGATATTTAATTGTAGGA+AGG 0.500952 2_3:+65642456 None:intergenic
TGTTATCATACTTGTCAACT+TGG 0.503441 2_3:-65640344 Msa0306610:three_prime_UTR
GATATTTAATTGTAGGAAGG+AGG 0.504732 2_3:+65642459 None:intergenic
AGTTGAATTGGTGAGCTCAA+TGG 0.520253 2_3:-65640568 Msa0306610:three_prime_UTR
AATTAAGAGAGGGAGTAGGA+AGG 0.531811 2_3:+65642494 None:intergenic
GGTTGGGCCGGCAAAAGAGC+CGG 0.542911 2_3:-65641036 Msa0306610:CDS
GACAAGAAGTTGACAGTGGT+GGG 0.556215 2_3:-65641122 Msa0306610:CDS
GTGTTTCAATTCACACATCC+AGG 0.563797 2_3:-65640394 Msa0306610:three_prime_UTR
TGATAGTATTGAGTCATGTA+GGG 0.568680 2_3:+65640900 None:intergenic
TAGAAGATTTGGATTTAATG+CGG 0.573195 2_3:-65640796 Msa0306610:three_prime_UTR
GATGGAATGCAACTTCGTGT+TGG 0.579616 2_3:+65642552 None:intergenic
AGACAAGAAGTTGACAGTGG+TGG 0.596259 2_3:-65641123 Msa0306610:CDS
TTGGACTCAAGTGAAAATGA+AGG 0.600404 2_3:-65642412 Msa0306610:intron
CCTCTGACTACATGAATGTG+TGG 0.619190 2_3:-65640620 Msa0306610:three_prime_UTR
AGGCATTGATTCTATCGCCA+TGG 0.631632 2_3:-65641153 Msa0306610:CDS
CCACACATTCATGTAGTCAG+AGG 0.634164 2_3:+65640620 None:intergenic
TAGATTGAGACGTCCTGATG+AGG 0.634492 2_3:-65640467 Msa0306610:three_prime_UTR
CACGCTATTAGGAGTGTCCG+TGG 0.642475 2_3:-65640756 Msa0306610:three_prime_UTR
ATCATTGGATTGAGATCACA+CGG 0.642508 2_3:+65640651 None:intergenic
GTGACCAGTGAGTACCTGAA+AGG 0.659865 2_3:+65641806 None:intergenic
GAGCTTATTAAGCAATACAG+AGG 0.663132 2_3:-65640930 Msa0306610:CDS
CAATTTCAATCCAATGACCA+CGG 0.698408 2_3:+65640739 None:intergenic
ATTTGCTCACAACATCAACA+GGG 0.737533 2_3:+65641090 None:intergenic
GAAAGACAAGAAGTTGACAG+TGG 0.740845 2_3:-65641126 Msa0306610:CDS
AATGTGTGGATTGTTAATTG+CGG 0.752410 2_3:-65640606 Msa0306610:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATTTATTAATTATT+TGG - chr2_3:65641423-65641442 Msa0306610:intron 0.0%
!! ATATTTATTTATTAATTATT+TGG - chr2_3:65641423-65641442 Msa0306610:intron 0.0%
!! ATTAATTAAACTATTATAGT+TGG - chr2_3:65640793-65640812 Msa0306610:three_prime_UTR 10.0%
!! ATTTACTATTACTAAATTAA+TGG + chr2_3:65641176-65641195 None:intergenic 10.0%
!! ATTAATTAAACTATTATAGT+TGG - chr2_3:65640793-65640812 Msa0306610:three_prime_UTR 10.0%
!! ATTTACTATTACTAAATTAA+TGG + chr2_3:65641176-65641195 None:intergenic 10.0%
!! ATTTCATATCAATTCAAATA+AGG - chr2_3:65640656-65640675 Msa0306610:three_prime_UTR 15.0%
!! TTTAATTAATGAAACAAGTA+AGG + chr2_3:65640783-65640802 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG - chr2_3:65641014-65641033 Msa0306610:CDS 15.0%
!! ATTAATAAATAAATATCTGC+TGG + chr2_3:65641419-65641438 None:intergenic 15.0%
!!! ATGTATTTTTACACATAAAT+GGG + chr2_3:65641669-65641688 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG + chr2_3:65641702-65641721 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG + chr2_3:65641756-65641775 None:intergenic 15.0%
!! TATGATAACACAAATTTAAA+TGG + chr2_3:65642577-65642596 None:intergenic 15.0%
!! ATTTCATATCAATTCAAATA+AGG - chr2_3:65640656-65640675 Msa0306610:three_prime_UTR 15.0%
!! TTTAATTAATGAAACAAGTA+AGG + chr2_3:65640783-65640802 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG - chr2_3:65641014-65641033 Msa0306610:CDS 15.0%
!! ATTAATAAATAAATATCTGC+TGG + chr2_3:65641419-65641438 None:intergenic 15.0%
!!! ATGTATTTTTACACATAAAT+GGG + chr2_3:65641669-65641688 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG + chr2_3:65641702-65641721 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG + chr2_3:65641756-65641775 None:intergenic 15.0%
!! TATGATAACACAAATTTAAA+TGG + chr2_3:65642577-65642596 None:intergenic 15.0%
!! TGAATAGAAAAATTAAGAGA+GGG + chr2_3:65640450-65640469 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG + chr2_3:65640482-65640501 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG + chr2_3:65640641-65640660 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG + chr2_3:65640686-65640705 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG + chr2_3:65641169-65641188 None:intergenic 20.0%
!! TTAATGGAATTTGTACTAAA+AGG + chr2_3:65641207-65641226 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG + chr2_3:65641223-65641242 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG - chr2_3:65641389-65641408 Msa0306610:intron 20.0%
!!! GATGTATTTTTACACATAAA+TGG + chr2_3:65641670-65641689 None:intergenic 20.0%
!! GAAAAATTAAACAATAAGCT+TGG - chr2_3:65642249-65642268 Msa0306610:intron 20.0%
!! TGAATAGAAAAATTAAGAGA+GGG + chr2_3:65640450-65640469 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG + chr2_3:65640482-65640501 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG + chr2_3:65640641-65640660 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG + chr2_3:65640686-65640705 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG + chr2_3:65641169-65641188 None:intergenic 20.0%
!! TTAATGGAATTTGTACTAAA+AGG + chr2_3:65641207-65641226 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG + chr2_3:65641223-65641242 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG - chr2_3:65641389-65641408 Msa0306610:intron 20.0%
!!! GATGTATTTTTACACATAAA+TGG + chr2_3:65641670-65641689 None:intergenic 20.0%
!! GAAAAATTAAACAATAAGCT+TGG - chr2_3:65642249-65642268 Msa0306610:intron 20.0%
! GTGAATAGAAAAATTAAGAG+AGG + chr2_3:65640451-65640470 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG + chr2_3:65640478-65640497 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG - chr2_3:65640545-65640564 Msa0306610:three_prime_UTR 25.0%
! TCAAAGAAAAAAACAGAACA+TGG + chr2_3:65640597-65640616 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG + chr2_3:65640631-65640650 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG + chr2_3:65640718-65640737 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG + chr2_3:65640721-65640740 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG + chr2_3:65640776-65640795 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG + chr2_3:65640777-65640796 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG + chr2_3:65640969-65640988 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG - chr2_3:65641044-65641063 Msa0306610:CDS 25.0%
! CCTTTGTTAATTAAAATGGA+CGG - chr2_3:65641393-65641412 Msa0306610:intron 25.0%
!!! ATATGATTTTTTGCTTGCTT+TGG + chr2_3:65641610-65641629 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG - chr2_3:65641758-65641777 Msa0306610:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG - chr2_3:65642124-65642143 Msa0306610:intron 25.0%
! TAGAAGATTTGGATTTAATG+CGG - chr2_3:65642135-65642154 Msa0306610:intron 25.0%
! ATCTAAATCCATAGTTGAAT+TGG - chr2_3:65642351-65642370 Msa0306610:intron 25.0%
! TTGCGTGTAAATTATGAATT+AGG - chr2_3:65642420-65642439 Msa0306610:five_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG - chr2_3:65642485-65642504 Msa0306610:five_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG - chr2_3:65642494-65642513 Msa0306610:five_prime_UTR 25.0%
! GTGAATAGAAAAATTAAGAG+AGG + chr2_3:65640451-65640470 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG + chr2_3:65640478-65640497 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG - chr2_3:65640545-65640564 Msa0306610:three_prime_UTR 25.0%
! TCAAAGAAAAAAACAGAACA+TGG + chr2_3:65640597-65640616 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG + chr2_3:65640631-65640650 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG + chr2_3:65640718-65640737 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG + chr2_3:65640721-65640740 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG + chr2_3:65640776-65640795 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG + chr2_3:65640777-65640796 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG + chr2_3:65640969-65640988 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG - chr2_3:65641044-65641063 Msa0306610:CDS 25.0%
! CCTTTGTTAATTAAAATGGA+CGG - chr2_3:65641393-65641412 Msa0306610:intron 25.0%
!!! ATATGATTTTTTGCTTGCTT+TGG + chr2_3:65641610-65641629 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG - chr2_3:65641758-65641777 Msa0306610:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG - chr2_3:65642124-65642143 Msa0306610:intron 25.0%
! TAGAAGATTTGGATTTAATG+CGG - chr2_3:65642135-65642154 Msa0306610:intron 25.0%
! ATCTAAATCCATAGTTGAAT+TGG - chr2_3:65642351-65642370 Msa0306610:intron 25.0%
! TTGCGTGTAAATTATGAATT+AGG - chr2_3:65642420-65642439 Msa0306610:five_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG - chr2_3:65642485-65642504 Msa0306610:five_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG - chr2_3:65642494-65642513 Msa0306610:five_prime_UTR 25.0%
AAGGATTGAATGTTTAGAAG+TGG + chr2_3:65640421-65640440 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG + chr2_3:65640475-65640494 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG - chr2_3:65640500-65640519 Msa0306610:three_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG + chr2_3:65640775-65640794 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG - chr2_3:65640889-65640908 Msa0306610:CDS 30.0%
CAGGAAAATGAAATCATTGT+TGG + chr2_3:65641323-65641342 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG - chr2_3:65641337-65641356 Msa0306610:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG + chr2_3:65641396-65641415 None:intergenic 30.0%
ATTCAAAAAACATCACTCCA+TGG + chr2_3:65641467-65641486 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG - chr2_3:65641576-65641595 Msa0306610:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG - chr2_3:65641710-65641729 Msa0306610:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG - chr2_3:65641855-65641874 Msa0306610:CDS 30.0%
! TTGGCACACAGAAATTTTAA+CGG - chr2_3:65641874-65641893 Msa0306610:CDS 30.0%
!! ACACAGAAATTTTAACGGTT+GGG - chr2_3:65641879-65641898 Msa0306610:intron 30.0%
! TGATAGTATTGAGTCATGTA+GGG + chr2_3:65642034-65642053 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG + chr2_3:65642035-65642054 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG + chr2_3:65642078-65642097 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG + chr2_3:65642116-65642135 None:intergenic 30.0%
! AATGTGTGGATTGTTAATTG+CGG - chr2_3:65642325-65642344 Msa0306610:intron 30.0%
TTGGACATGAACAAAAAGTA+AGG - chr2_3:65642392-65642411 Msa0306610:intron 30.0%
TGTTATCATACTTGTCAACT+TGG - chr2_3:65642587-65642606 Msa0306610:five_prime_UTR 30.0%
AAGGATTGAATGTTTAGAAG+TGG + chr2_3:65640421-65640440 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG + chr2_3:65640475-65640494 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG - chr2_3:65640500-65640519 Msa0306610:three_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG + chr2_3:65640775-65640794 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG - chr2_3:65640889-65640908 Msa0306610:CDS 30.0%
CAGGAAAATGAAATCATTGT+TGG + chr2_3:65641323-65641342 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG - chr2_3:65641337-65641356 Msa0306610:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG + chr2_3:65641396-65641415 None:intergenic 30.0%
ATTCAAAAAACATCACTCCA+TGG + chr2_3:65641467-65641486 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG - chr2_3:65641576-65641595 Msa0306610:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG - chr2_3:65641710-65641729 Msa0306610:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG - chr2_3:65641855-65641874 Msa0306610:CDS 30.0%
! TTGGCACACAGAAATTTTAA+CGG - chr2_3:65641874-65641893 Msa0306610:CDS 30.0%
!! ACACAGAAATTTTAACGGTT+GGG - chr2_3:65641879-65641898 Msa0306610:intron 30.0%
! TGATAGTATTGAGTCATGTA+GGG + chr2_3:65642034-65642053 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG + chr2_3:65642035-65642054 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG + chr2_3:65642078-65642097 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG + chr2_3:65642116-65642135 None:intergenic 30.0%
! AATGTGTGGATTGTTAATTG+CGG - chr2_3:65642325-65642344 Msa0306610:intron 30.0%
TTGGACATGAACAAAAAGTA+AGG - chr2_3:65642392-65642411 Msa0306610:intron 30.0%
TGTTATCATACTTGTCAACT+TGG - chr2_3:65642587-65642606 Msa0306610:five_prime_UTR 30.0%
TGAATGTTTAGAAGTGGAGA+AGG + chr2_3:65640415-65640434 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG + chr2_3:65640444-65640463 None:intergenic 35.0%
TTGGACTCAAGTGAAAATGA+AGG - chr2_3:65640519-65640538 Msa0306610:three_prime_UTR 35.0%
GTAGAAAATAGTGGTGAAGT+TGG - chr2_3:65641053-65641072 Msa0306610:CDS 35.0%
AAAGCAGAAAGCCATGAAAT+CGG - chr2_3:65641095-65641114 Msa0306610:CDS 35.0%
GGAATTTGTACTAAAAGGAC+AGG + chr2_3:65641202-65641221 None:intergenic 35.0%
GTCCAATTGTATACTTACTC+AGG + chr2_3:65641342-65641361 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG - chr2_3:65641447-65641466 Msa0306610:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG - chr2_3:65641527-65641546 Msa0306610:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG - chr2_3:65641528-65641547 Msa0306610:intron 35.0%
ACACATTGTCATGTATGAGT+CGG + chr2_3:65641572-65641591 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG - chr2_3:65641581-65641600 Msa0306610:intron 35.0%
ATTTGCTCACAACATCAACA+GGG + chr2_3:65641844-65641863 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG + chr2_3:65641845-65641864 None:intergenic 35.0%
!! CACACAGAAATTTTAACGGT+TGG - chr2_3:65641878-65641897 Msa0306610:intron 35.0%
! AGCTCAGCAATTTGTTCATT+TGG + chr2_3:65641986-65642005 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG - chr2_3:65642001-65642020 Msa0306610:intron 35.0%
CAACAGATAACACAAGCATT+TGG + chr2_3:65642079-65642098 None:intergenic 35.0%
TTTCACACATTCACGCTATT+AGG - chr2_3:65642164-65642183 Msa0306610:intron 35.0%
CAATTTCAATCCAATGACCA+CGG + chr2_3:65642195-65642214 None:intergenic 35.0%
ATCATTGGATTGAGATCACA+CGG + chr2_3:65642283-65642302 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG + chr2_3:65642480-65642499 None:intergenic 35.0%
TGAATGTTTAGAAGTGGAGA+AGG + chr2_3:65640415-65640434 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG + chr2_3:65640444-65640463 None:intergenic 35.0%
TTGGACTCAAGTGAAAATGA+AGG - chr2_3:65640519-65640538 Msa0306610:three_prime_UTR 35.0%
GTAGAAAATAGTGGTGAAGT+TGG - chr2_3:65641053-65641072 Msa0306610:CDS 35.0%
AAAGCAGAAAGCCATGAAAT+CGG - chr2_3:65641095-65641114 Msa0306610:CDS 35.0%
GGAATTTGTACTAAAAGGAC+AGG + chr2_3:65641202-65641221 None:intergenic 35.0%
GTCCAATTGTATACTTACTC+AGG + chr2_3:65641342-65641361 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG - chr2_3:65641447-65641466 Msa0306610:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG - chr2_3:65641527-65641546 Msa0306610:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG - chr2_3:65641528-65641547 Msa0306610:intron 35.0%
ACACATTGTCATGTATGAGT+CGG + chr2_3:65641572-65641591 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG - chr2_3:65641581-65641600 Msa0306610:intron 35.0%
ATTTGCTCACAACATCAACA+GGG + chr2_3:65641844-65641863 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG + chr2_3:65641845-65641864 None:intergenic 35.0%
!! CACACAGAAATTTTAACGGT+TGG - chr2_3:65641878-65641897 Msa0306610:intron 35.0%
! AGCTCAGCAATTTGTTCATT+TGG + chr2_3:65641986-65642005 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG - chr2_3:65642001-65642020 Msa0306610:intron 35.0%
CAACAGATAACACAAGCATT+TGG + chr2_3:65642079-65642098 None:intergenic 35.0%
TTTCACACATTCACGCTATT+AGG - chr2_3:65642164-65642183 Msa0306610:intron 35.0%
CAATTTCAATCCAATGACCA+CGG + chr2_3:65642195-65642214 None:intergenic 35.0%
ATCATTGGATTGAGATCACA+CGG + chr2_3:65642283-65642302 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG + chr2_3:65642480-65642499 None:intergenic 35.0%
!!! GGCACTTGTTTGACTTGTTT+TGG - chr2_3:65640312-65640331 Msa0306610:three_prime_UTR 40.0%
ATGGAATGCAACTTCGTGTT+GGG + chr2_3:65640381-65640400 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG + chr2_3:65640440-65640459 None:intergenic 40.0%
! AAGGCTAGAAACCGATTTCA+TGG + chr2_3:65641109-65641128 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG - chr2_3:65641111-65641130 Msa0306610:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG - chr2_3:65641529-65641548 Msa0306610:intron 40.0%
ACATAAATGGGACAACTGCA+AGG + chr2_3:65641657-65641676 None:intergenic 40.0%
! TGTCCGATTGAGACATGTTT+TGG + chr2_3:65641716-65641735 None:intergenic 40.0%
CTTCTTGTCTTTCATGTCCA+TGG + chr2_3:65641798-65641817 None:intergenic 40.0%
GAAAGACAAGAAGTTGACAG+TGG - chr2_3:65641805-65641824 Msa0306610:intron 40.0%
!! AGAAATTTTAACGGTTGGGC+CGG - chr2_3:65641883-65641902 Msa0306610:intron 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG + chr2_3:65641917-65641936 None:intergenic 40.0%
CGTGGTCATTGGATTGAAAT+TGG - chr2_3:65642193-65642212 Msa0306610:intron 40.0%
TGAGCTCACCAATTCAACTA+TGG + chr2_3:65642362-65642381 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG - chr2_3:65642363-65642382 Msa0306610:intron 40.0%
GTGAGCTCAATGGAATTGAT+TGG - chr2_3:65642373-65642392 Msa0306610:intron 40.0%
GTGTTTCAATTCACACATCC+AGG - chr2_3:65642537-65642556 Msa0306610:five_prime_UTR 40.0%
!!! GGCACTTGTTTGACTTGTTT+TGG - chr2_3:65640312-65640331 Msa0306610:three_prime_UTR 40.0%
ATGGAATGCAACTTCGTGTT+GGG + chr2_3:65640381-65640400 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG + chr2_3:65640440-65640459 None:intergenic 40.0%
! AAGGCTAGAAACCGATTTCA+TGG + chr2_3:65641109-65641128 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG - chr2_3:65641111-65641130 Msa0306610:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG - chr2_3:65641529-65641548 Msa0306610:intron 40.0%
ACATAAATGGGACAACTGCA+AGG + chr2_3:65641657-65641676 None:intergenic 40.0%
! TGTCCGATTGAGACATGTTT+TGG + chr2_3:65641716-65641735 None:intergenic 40.0%
CTTCTTGTCTTTCATGTCCA+TGG + chr2_3:65641798-65641817 None:intergenic 40.0%
GAAAGACAAGAAGTTGACAG+TGG - chr2_3:65641805-65641824 Msa0306610:intron 40.0%
!! AGAAATTTTAACGGTTGGGC+CGG - chr2_3:65641883-65641902 Msa0306610:intron 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG + chr2_3:65641917-65641936 None:intergenic 40.0%
CGTGGTCATTGGATTGAAAT+TGG - chr2_3:65642193-65642212 Msa0306610:intron 40.0%
TGAGCTCACCAATTCAACTA+TGG + chr2_3:65642362-65642381 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG - chr2_3:65642363-65642382 Msa0306610:intron 40.0%
GTGAGCTCAATGGAATTGAT+TGG - chr2_3:65642373-65642392 Msa0306610:intron 40.0%
GTGTTTCAATTCACACATCC+AGG - chr2_3:65642537-65642556 Msa0306610:five_prime_UTR 40.0%
GATGGAATGCAACTTCGTGT+TGG + chr2_3:65640382-65640401 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG + chr2_3:65640578-65640597 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG - chr2_3:65641138-65641157 Msa0306610:CDS 45.0%
ACAGTTGTCCCCAAAACGAA+CGG + chr2_3:65641540-65641559 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG - chr2_3:65641778-65641797 Msa0306610:intron 45.0%
AGACAAGAAGTTGACAGTGG+TGG - chr2_3:65641808-65641827 Msa0306610:intron 45.0%
GACAAGAAGTTGACAGTGGT+GGG - chr2_3:65641809-65641828 Msa0306610:intron 45.0%
CCGGAGAAGAAAAAAGACGA+AGG - chr2_3:65641914-65641933 Msa0306610:intron 45.0%
!! TCAGAGGCGTCACTAATCAT+TGG + chr2_3:65642298-65642317 None:intergenic 45.0%
CCACACATTCATGTAGTCAG+AGG + chr2_3:65642314-65642333 None:intergenic 45.0%
CCTCTGACTACATGAATGTG+TGG - chr2_3:65642311-65642330 Msa0306610:intron 45.0%
TAGATTGAGACGTCCTGATG+AGG - chr2_3:65642464-65642483 Msa0306610:five_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG - chr2_3:65642471-65642490 Msa0306610:five_prime_UTR 45.0%
ATGGCACAATGAACAAGTCC+TGG + chr2_3:65642558-65642577 None:intergenic 45.0%
GATGGAATGCAACTTCGTGT+TGG + chr2_3:65640382-65640401 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG + chr2_3:65640578-65640597 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG - chr2_3:65641138-65641157 Msa0306610:CDS 45.0%
ACAGTTGTCCCCAAAACGAA+CGG + chr2_3:65641540-65641559 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG - chr2_3:65641778-65641797 Msa0306610:intron 45.0%
AGACAAGAAGTTGACAGTGG+TGG - chr2_3:65641808-65641827 Msa0306610:intron 45.0%
GACAAGAAGTTGACAGTGGT+GGG - chr2_3:65641809-65641828 Msa0306610:intron 45.0%
CCGGAGAAGAAAAAAGACGA+AGG - chr2_3:65641914-65641933 Msa0306610:intron 45.0%
!! TCAGAGGCGTCACTAATCAT+TGG + chr2_3:65642298-65642317 None:intergenic 45.0%
CCACACATTCATGTAGTCAG+AGG + chr2_3:65642314-65642333 None:intergenic 45.0%
CCTCTGACTACATGAATGTG+TGG - chr2_3:65642311-65642330 Msa0306610:intron 45.0%
TAGATTGAGACGTCCTGATG+AGG - chr2_3:65642464-65642483 Msa0306610:five_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG - chr2_3:65642471-65642490 Msa0306610:five_prime_UTR 45.0%
ATGGCACAATGAACAAGTCC+TGG + chr2_3:65642558-65642577 None:intergenic 45.0%
!! ATATCAAAAATAAATAATAA+AGG - chr2_3:65641626-65641645 Msa0306610:intron 5.0%
!! ATATCAAAAATAAATAATAA+AGG - chr2_3:65641626-65641645 Msa0306610:intron 5.0%
GGAGAAGGAGCAGAGAAAGA+TGG + chr2_3:65640400-65640419 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG - chr2_3:65641121-65641140 Msa0306610:CDS 50.0%
GTGACCAGTGAGTACCTGAA+AGG + chr2_3:65641128-65641147 None:intergenic 50.0%
AAATGGGACAACTGCAAGGC+TGG + chr2_3:65641653-65641672 None:intergenic 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG + chr2_3:65641905-65641924 None:intergenic 50.0%
!! TTAGGAGTGTCCGTGGTCAT+TGG - chr2_3:65642182-65642201 Msa0306610:intron 50.0%
GGAGAAGGAGCAGAGAAAGA+TGG + chr2_3:65640400-65640419 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG - chr2_3:65641121-65641140 Msa0306610:CDS 50.0%
GTGACCAGTGAGTACCTGAA+AGG + chr2_3:65641128-65641147 None:intergenic 50.0%
AAATGGGACAACTGCAAGGC+TGG + chr2_3:65641653-65641672 None:intergenic 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG + chr2_3:65641905-65641924 None:intergenic 50.0%
!! TTAGGAGTGTCCGTGGTCAT+TGG - chr2_3:65642182-65642201 Msa0306610:intron 50.0%
!! CACGCTATTAGGAGTGTCCG+TGG - chr2_3:65642175-65642194 Msa0306610:intron 55.0%
!! CACGCTATTAGGAGTGTCCG+TGG - chr2_3:65642175-65642194 Msa0306610:intron 55.0%
GGTTGGGCCGGCAAAAGAGC+CGG - chr2_3:65641895-65641914 Msa0306610:intron 65.0%
GGTTGGGCCGGCAAAAGAGC+CGG - chr2_3:65641895-65641914 Msa0306610:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 65640308 65642645 65640308 ID=Msa0306610;Name=Msa0306610
chr2_3 mRNA 65640308 65642645 65640308 ID=Msa0306610-mRNA-1;Parent=Msa0306610;Name=Msa0306610-mRNA-1;_AED=0.07;_eAED=0.07;_QI=227|1|1|1|1|1|3|546|133
chr2_3 exon 65640308 65641173 65640308 ID=Msa0306610-mRNA-1:exon:21853;Parent=Msa0306610-mRNA-1
chr2_3 exon 65641821 65641896 65641821 ID=Msa0306610-mRNA-1:exon:21852;Parent=Msa0306610-mRNA-1
chr2_3 exon 65642413 65642645 65642413 ID=Msa0306610-mRNA-1:exon:21851;Parent=Msa0306610-mRNA-1
chr2_3 five_prime_UTR 65642419 65642645 65642419 ID=Msa0306610-mRNA-1:five_prime_utr;Parent=Msa0306610-mRNA-1
chr2_3 CDS 65642413 65642418 65642413 ID=Msa0306610-mRNA-1:cds;Parent=Msa0306610-mRNA-1
chr2_3 CDS 65641821 65641896 65641821 ID=Msa0306610-mRNA-1:cds;Parent=Msa0306610-mRNA-1
chr2_3 CDS 65640854 65641173 65640854 ID=Msa0306610-mRNA-1:cds;Parent=Msa0306610-mRNA-1
chr2_3 three_prime_UTR 65640308 65640853 65640308 ID=Msa0306610-mRNA-1:three_prime_utr;Parent=Msa0306610-mRNA-1
Gene Sequence

>Msa0306610

ATGAAGAAAATAGTGGTGAAGTTGGACTTGCATGATGACAAAGCAAAGCAGAAAGCCATGAAATCGGTTTCTAGCCTTTCAGGCATTGATTCTATCGCCATGGACATGAAAGACAAGAAGTTGACAGTGGTGGGCAACATAGACCCTGTTGATGTTGTGAGCAAATTGAGAAAAACTTGGCACACAGAAATTTTAACGGTTGGGCCGGCAAAAGAGCCGGAGAAGAAAAAAGACGAAGGCAAAAAAGATGATAATAAGAAGAAAGAAGATGACAAGAAGAAAGATCCAAATGAACAAATTGCTGAGCTTATTAAGCAATACAGAGGATACAATCCCTACATGACTCAATACTATCATGTTCAGAGTGTAGAAGAGAACCCAAATGCTTGTGTTATCTGTTGA
Protein sequence

>Msa0306610

MKKIVVKLDLHDDKAKQKAMKSVSSLSGIDSIAMDMKDKKLTVVGNIDPVDVVSKLRKTWHTEILTVGPAKEPEKKKDEGKKDDNKKKEDDKKKDPNEQIAELIKQYRGYNPYMTQYYHVQSVEENPNACVIC*