AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576900 MtrunA17_Chr8g0387471 92.481 133 4 1 1 133 1 127 4.21e-85 244
Msa0576900 MtrunA17_Chr8g0348361 53.691 149 53 1 1 133 1 149 6.68e-37 123
Msa0576900 MtrunA17_Chr6g0469651 52.941 136 58 2 1 133 1 133 2.09e-33 114
Msa0576900 MtrunA17_Chr6g0469681 50.000 138 53 3 1 133 1 127 5.18e-33 112
Msa0576900 MtrunA17_Chr6g0469571 48.120 133 60 1 1 133 1 124 1.41e-32 111
Msa0576900 MtrunA17_Chr6g0469551 47.368 133 61 1 1 133 1 124 4.16e-32 110
Msa0576900 MtrunA17_Chr1g0202541 47.407 135 68 2 1 132 1 135 4.28e-32 110
Msa0576900 MtrunA17_Chr6g0469591 46.617 133 62 1 1 133 1 124 2.06e-30 105
Msa0576900 MtrunA17_Chr1g0202561 49.306 144 55 4 1 132 1 138 5.20e-29 102
Msa0576900 MtrunA17_Chr1g0202501 50.000 134 62 3 1 133 1 130 5.55e-29 102
Msa0576900 MtrunA17_Chr1g0202581 49.242 132 66 1 1 132 1 131 7.45e-29 102
Msa0576900 MtrunA17_Chr7g0248671 73.239 71 19 0 1 71 1 71 3.00e-28 102
Msa0576900 MtrunA17_Chr1g0204291 45.000 140 60 3 1 132 1 131 7.39e-28 99.8
Msa0576900 MtrunA17_Chr6g0469671 62.857 70 26 0 1 70 1 70 1.23e-27 98.6
Msa0576900 MtrunA17_Chr7g0233181 54.667 75 34 0 1 75 1 75 1.41e-24 90.5
Msa0576900 MtrunA17_Chr7g0233251 54.667 75 34 0 1 75 1 75 3.08e-24 89.7
Msa0576900 MtrunA17_Chr6g0469541 48.750 80 41 0 1 80 1 80 7.47e-24 89.0
Msa0576900 MtrunA17_Chr7g0233191 40.496 121 60 2 1 120 1 110 8.99e-24 88.6
Msa0576900 MtrunA17_Chr7g0233211 54.430 79 34 1 1 79 1 77 1.08e-23 88.6
Msa0576900 MtrunA17_Chr7g0233201 40.496 121 60 2 1 120 1 110 1.30e-23 88.2
Msa0576900 MtrunA17_Chr7g0233231 53.333 75 35 0 1 75 1 75 9.00e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576900 AT1G01490.1 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa0576900 AT1G01490.2 49.153 177 46 3 1 133 1 177 8.60e-44 142
Msa0576900 AT1G01490.4 48.571 175 46 3 3 133 30 204 1.41e-42 139
Msa0576900 AT1G01490.3 40.278 144 42 3 34 133 1 144 2.81e-21 83.6
Msa0576900 AT5G52740.1 43.609 133 56 4 1 131 1 116 6.51e-19 76.6
Msa0576900 AT5G52760.1 38.017 121 57 4 2 119 4 109 5.34e-16 69.7
Msa0576900 AT1G63950.2 44.776 67 37 0 1 67 1 67 1.76e-13 62.8
Msa0576900 AT1G63960.2 40.000 70 41 1 1 69 11 80 2.22e-12 60.5
Msa0576900 AT1G63950.1 42.188 64 37 0 4 67 1 64 1.18e-11 57.8
Msa0576900 AT5G52750.1 43.077 65 37 0 3 67 5 69 4.33e-11 57.0

Find 59 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCGGTTTCTAGCCTTTC+AGG 0.141123 4_1:-81059010 Msa0576900:intron
GGCACTTGTTTGACTTGTTT+TGG 0.244120 4_1:-81059823 Msa0576900:five_prime_UTR
AGTGGTCATTGGATTGAAAT+TGG 0.257554 4_1:-81057913 Msa0576900:three_prime_UTR
AGCTCAGCAATTTGTTCATT+TGG 0.261412 4_1:+81058127 None:intergenic
ATTCATGCATTCATGTTATT+AGG 0.265433 4_1:-81057942 Msa0576900:three_prime_UTR
ATGATAACACAAACTTTAAA+TGG 0.284136 4_1:+81057532 None:intergenic
TTGCGTGTAAATTATGAATT+AGG 0.295560 4_1:-81057686 Msa0576900:three_prime_UTR
ATCTAAATCCATAGTTGAAT+TGG 0.318872 4_1:-81057755 Msa0576900:three_prime_UTR
AAAGCATAGAATAGAAGATT+TGG 0.328856 4_1:-81057982 Msa0576900:three_prime_UTR
CAACAGATAACACAAGCATT+TGG 0.350285 4_1:+81058034 None:intergenic
GGATTATGGTGTATTAAAAT+AGG 0.352846 4_1:-81057621 Msa0576900:three_prime_UTR
TTGACTGATCTTCAAAACTT+TGG 0.364724 4_1:-81059635 Msa0576900:five_prime_UTR
TGAGCTCACCAATTCAACTA+TGG 0.367558 4_1:+81057747 None:intergenic
AAGTGTTGATATTTAATTGT+AGG 0.377594 4_1:+81059656 None:intergenic
AATTTGCTCACAACATCAAC+AGG 0.390553 4_1:+81058268 None:intergenic
AAGGCTAGAAACCGATTTCA+TGG 0.398452 4_1:+81059015 None:intergenic
ATTCTATGCTTTCTCAATCA+AGG 0.399212 4_1:+81057993 None:intergenic
AACAGATAACACAAGCATTT+GGG 0.403989 4_1:+81058035 None:intergenic
TTAGGAGCGTCAGTGGTCAT+TGG 0.427114 4_1:-81057924 Msa0576900:three_prime_UTR
AGACGTCCTGATGAGGATTA+TGG 0.438970 4_1:-81057635 Msa0576900:three_prime_UTR
ATGATAGTATTGAGTCATGT+AGG 0.445771 4_1:+81058078 None:intergenic
TGTATTAAAATAGGCTATGA+AGG 0.452511 4_1:-81057612 Msa0576900:three_prime_UTR
GTGAGCTCAATGGAATTGAT+TGG 0.453628 4_1:-81057733 Msa0576900:three_prime_UTR
TCTCTGACTACATGAATGTA+TGG 0.459397 4_1:-81057795 Msa0576900:three_prime_UTR
GGAGAAGGAGCAGAGAAAGA+TGG 0.462159 4_1:+81059738 None:intergenic
ATGGAATGCAACTTCGTGTT+GGG 0.465202 4_1:+81059757 None:intergenic
TGAATGTTTAGAAGTGGAGA+AGG 0.466461 4_1:+81059723 None:intergenic
CTTCTTGTCCTTCATGTCCA+TGG 0.471694 4_1:+81058315 None:intergenic
TTTGTTCTTGTAGAAAATAG+TGG 0.476254 4_1:-81059077 Msa0576900:intron
AATACACCATAATCCTCATC+AGG 0.477270 4_1:+81057629 None:intergenic
TCAGAGACGTCACTAATCAT+TGG 0.479371 4_1:+81057811 None:intergenic
ATGGCACAATGAACAAGTCT+TGG 0.479626 4_1:+81057551 None:intergenic
GTAGAAAATAGTGGTGAAGT+TGG 0.484582 4_1:-81059068 Msa0576900:intron
AAGGATTGAATGTTTAGAAG+TGG 0.485327 4_1:+81059717 None:intergenic
AAAGCAGAAAGCCATGAAAT+CGG 0.493020 4_1:-81059026 Msa0576900:CDS
GTTGATATTTAATTGTAGGA+AGG 0.500952 4_1:+81059660 None:intergenic
TGTTATCATACTTGTCAACT+TGG 0.503441 4_1:-81057518 Msa0576900:three_prime_UTR
GATATTTAATTGTAGGAAGG+AGG 0.504732 4_1:+81059663 None:intergenic
AGTTGAATTGGTGAGCTCAA+TGG 0.520253 4_1:-81057743 Msa0576900:three_prime_UTR
TTCTATCGCCATGGACATGA+AGG 0.528603 4_1:-81058323 Msa0576900:CDS
TAGAAGATTTGGATTTAATG+TGG 0.529026 4_1:-81057971 Msa0576900:three_prime_UTR
AATTAAGAGAGGGAGTAGGA+AGG 0.531811 4_1:+81059698 None:intergenic
GGTTGGGCCGGCAAAAGAGC+CGG 0.542911 4_1:-81058215 Msa0576900:CDS
GGACAAGAAGCTGACAGTGG+TGG 0.567439 4_1:-81058302 Msa0576900:CDS
TGATAGTATTGAGTCATGTA+GGG 0.568680 4_1:+81058079 None:intergenic
GATGGAATGCAACTTCGTGT+TGG 0.579616 4_1:+81059756 None:intergenic
GACAAGAAGCTGACAGTGGT+GGG 0.581359 4_1:-81058301 Msa0576900:CDS
CATGTTATTAGGAGCGTCAG+TGG 0.586572 4_1:-81057931 Msa0576900:three_prime_UTR
AGGCATTGATTCTATCGCCA+TGG 0.631632 4_1:-81058332 Msa0576900:CDS
TTGGACTCGAGTGAAAATGA+AGG 0.633040 4_1:-81059616 Msa0576900:intron
TAGATTGAGACGTCCTGATG+AGG 0.634492 4_1:-81057642 Msa0576900:three_prime_UTR
TTCGTGTTGGGACAATATGT+AGG 0.635330 4_1:+81059769 None:intergenic
ATCATTGGATTGAGATCACA+CGG 0.642508 4_1:+81057826 None:intergenic
CTATCATGTTCAGAGTGTAG+AGG 0.659008 4_1:-81058062 Msa0576900:CDS
GTGACCAGTGAGTACCTGAA+AGG 0.659865 4_1:+81058996 None:intergenic
GAGCTTATTAAGCAATACAG+AGG 0.663132 4_1:-81058109 Msa0576900:CDS
AATGTATGGATTGTTAATTG+CGG 0.716916 4_1:-81057781 Msa0576900:three_prime_UTR
GAAGGACAAGAAGCTGACAG+TGG 0.724331 4_1:-81058305 Msa0576900:CDS
ATTTGCTCACAACATCAACA+GGG 0.737533 4_1:+81058269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATTTATTAATTATT+TGG - chr4_1:81058611-81058630 Msa0576900:intron 0.0%
!! ATATTTATTTATTAATTATT+TGG - chr4_1:81058611-81058630 Msa0576900:intron 0.0%
!! ATTAATTAAACTATTATAGT+TGG - chr4_1:81057988-81058007 Msa0576900:three_prime_UTR 10.0%
!! ATTTACTATTACTAAATTAA+TGG + chr4_1:81058364-81058383 None:intergenic 10.0%
!! ATTAATTAAACTATTATAGT+TGG - chr4_1:81057988-81058007 Msa0576900:three_prime_UTR 10.0%
!! ATTTACTATTACTAAATTAA+TGG + chr4_1:81058364-81058383 None:intergenic 10.0%
!! ATTTCATATCAATTCAAATA+AGG - chr4_1:81057830-81057849 Msa0576900:three_prime_UTR 15.0%
!! TTTAATTAATGAAACAAGTA+AGG + chr4_1:81057978-81057997 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG - chr4_1:81058201-81058220 Msa0576900:CDS 15.0%
!! ATTAATAAATAAATATCTGC+TGG + chr4_1:81058607-81058626 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG + chr4_1:81058901-81058920 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG + chr4_1:81058955-81058974 None:intergenic 15.0%
!! AAAAAATTAAACAATAAGCT+TGG - chr4_1:81059452-81059471 Msa0576900:intron 15.0%
!! ATTTCATATCAATTCAAATA+AGG - chr4_1:81057830-81057849 Msa0576900:three_prime_UTR 15.0%
!! TTTAATTAATGAAACAAGTA+AGG + chr4_1:81057978-81057997 None:intergenic 15.0%
!! TAATAATGATTTCAATTCTT+AGG - chr4_1:81058201-81058220 Msa0576900:CDS 15.0%
!! ATTAATAAATAAATATCTGC+TGG + chr4_1:81058607-81058626 None:intergenic 15.0%
!!! ATGTTTTGGTTTTTTATATT+TGG + chr4_1:81058901-81058920 None:intergenic 15.0%
!!! ATTCAAATCATTAACTATTT+TGG + chr4_1:81058955-81058974 None:intergenic 15.0%
!! AAAAAATTAAACAATAAGCT+TGG - chr4_1:81059452-81059471 Msa0576900:intron 15.0%
!! TGAATAGAAAAATTAAGAGA+GGG + chr4_1:81057624-81057643 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG + chr4_1:81057656-81057675 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG + chr4_1:81057815-81057834 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG + chr4_1:81057860-81057879 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG + chr4_1:81058357-81058376 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG + chr4_1:81058411-81058430 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG - chr4_1:81058577-81058596 Msa0576900:intron 20.0%
!! ATGATAACACAAACTTTAAA+TGG + chr4_1:81059780-81059799 None:intergenic 20.0%
!! TGAATAGAAAAATTAAGAGA+GGG + chr4_1:81057624-81057643 None:intergenic 20.0%
!!! AAGTGTTGATATTTAATTGT+AGG + chr4_1:81057656-81057675 None:intergenic 20.0%
!! GAAATAAACAAGAAATTGAA+AGG + chr4_1:81057815-81057834 None:intergenic 20.0%
!! AGAAATATTGAATACTTGAA+TGG + chr4_1:81057860-81057879 None:intergenic 20.0%
!! ATTACTAAATTAATGGAGTA+AGG + chr4_1:81058357-81058376 None:intergenic 20.0%
!!! TCGAGTTTTAAAATGTTTAA+TGG + chr4_1:81058411-81058430 None:intergenic 20.0%
!! TGAACCTTTGTTAATTAAAA+TGG - chr4_1:81058577-81058596 Msa0576900:intron 20.0%
!! ATGATAACACAAACTTTAAA+TGG + chr4_1:81059780-81059799 None:intergenic 20.0%
! GTGAATAGAAAAATTAAGAG+AGG + chr4_1:81057625-81057644 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG + chr4_1:81057652-81057671 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG - chr4_1:81057719-81057738 Msa0576900:three_prime_UTR 25.0%
! TCAAAGAAAAAAACAGAACA+TGG + chr4_1:81057771-81057790 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG + chr4_1:81057805-81057824 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG + chr4_1:81057910-81057929 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG + chr4_1:81057913-81057932 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG + chr4_1:81057971-81057990 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG + chr4_1:81057972-81057991 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG + chr4_1:81058156-81058175 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG - chr4_1:81058232-81058251 Msa0576900:CDS 25.0%
! CCTTTGTTAATTAAAATGGA+CGG - chr4_1:81058581-81058600 Msa0576900:intron 25.0%
! CACATAAATGTACACTAAAT+GGG + chr4_1:81058857-81058876 None:intergenic 25.0%
! ACACATAAATGTACACTAAA+TGG + chr4_1:81058858-81058877 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG - chr4_1:81058957-81058976 Msa0576900:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG - chr4_1:81059327-81059346 Msa0576900:intron 25.0%
! TAGAAGATTTGGATTTAATG+TGG - chr4_1:81059338-81059357 Msa0576900:intron 25.0%
! ATTCATGCATTCATGTTATT+AGG - chr4_1:81059367-81059386 Msa0576900:intron 25.0%
!! AATGTATGGATTGTTAATTG+CGG - chr4_1:81059528-81059547 Msa0576900:intron 25.0%
! ATCTAAATCCATAGTTGAAT+TGG - chr4_1:81059554-81059573 Msa0576900:intron 25.0%
! TTGCGTGTAAATTATGAATT+AGG - chr4_1:81059623-81059642 Msa0576900:five_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG - chr4_1:81059688-81059707 Msa0576900:five_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG - chr4_1:81059697-81059716 Msa0576900:five_prime_UTR 25.0%
! GTGAATAGAAAAATTAAGAG+AGG + chr4_1:81057625-81057644 None:intergenic 25.0%
! GTTGATATTTAATTGTAGGA+AGG + chr4_1:81057652-81057671 None:intergenic 25.0%
! GATTTCTTGCTCTAATTTAA+AGG - chr4_1:81057719-81057738 Msa0576900:three_prime_UTR 25.0%
! TCAAAGAAAAAAACAGAACA+TGG + chr4_1:81057771-81057790 None:intergenic 25.0%
! AGAAATTGAAAGGAAAAAAG+AGG + chr4_1:81057805-81057824 None:intergenic 25.0%
! AAAAAAAAAACTGAGTAAGG+TGG + chr4_1:81057910-81057929 None:intergenic 25.0%
! CTGAAAAAAAAAACTGAGTA+AGG + chr4_1:81057913-81057932 None:intergenic 25.0%
! AATGAAACAAGTAAGGAAAT+GGG + chr4_1:81057971-81057990 None:intergenic 25.0%
! TAATGAAACAAGTAAGGAAA+TGG + chr4_1:81057972-81057991 None:intergenic 25.0%
! ATGTAATGAAAAAACAAAGC+AGG + chr4_1:81058156-81058175 None:intergenic 25.0%
! TTTGTTCTTGTAGAAAATAG+TGG - chr4_1:81058232-81058251 Msa0576900:CDS 25.0%
! CCTTTGTTAATTAAAATGGA+CGG - chr4_1:81058581-81058600 Msa0576900:intron 25.0%
! CACATAAATGTACACTAAAT+GGG + chr4_1:81058857-81058876 None:intergenic 25.0%
! ACACATAAATGTACACTAAA+TGG + chr4_1:81058858-81058877 None:intergenic 25.0%
! ATAGTTAATGATTTGAATGC+AGG - chr4_1:81058957-81058976 Msa0576900:intron 25.0%
! AAAGCATAGAATAGAAGATT+TGG - chr4_1:81059327-81059346 Msa0576900:intron 25.0%
! TAGAAGATTTGGATTTAATG+TGG - chr4_1:81059338-81059357 Msa0576900:intron 25.0%
! ATTCATGCATTCATGTTATT+AGG - chr4_1:81059367-81059386 Msa0576900:intron 25.0%
!! AATGTATGGATTGTTAATTG+CGG - chr4_1:81059528-81059547 Msa0576900:intron 25.0%
! ATCTAAATCCATAGTTGAAT+TGG - chr4_1:81059554-81059573 Msa0576900:intron 25.0%
! TTGCGTGTAAATTATGAATT+AGG - chr4_1:81059623-81059642 Msa0576900:five_prime_UTR 25.0%
! GGATTATGGTGTATTAAAAT+AGG - chr4_1:81059688-81059707 Msa0576900:five_prime_UTR 25.0%
! TGTATTAAAATAGGCTATGA+AGG - chr4_1:81059697-81059716 Msa0576900:five_prime_UTR 25.0%
AAGGATTGAATGTTTAGAAG+TGG + chr4_1:81057595-81057614 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG + chr4_1:81057649-81057668 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG - chr4_1:81057674-81057693 Msa0576900:three_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG + chr4_1:81057970-81057989 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG - chr4_1:81058076-81058095 Msa0576900:CDS 30.0%
GGAATTTGTACTAAAATGAC+AGG + chr4_1:81058390-81058409 None:intergenic 30.0%
CAGGAAAATGAAATCATTGT+TGG + chr4_1:81058511-81058530 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG - chr4_1:81058525-81058544 Msa0576900:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG + chr4_1:81058584-81058603 None:intergenic 30.0%
ATTCAAATAACATCACTCCA+TGG + chr4_1:81058655-81058674 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG - chr4_1:81058764-81058783 Msa0576900:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG - chr4_1:81058909-81058928 Msa0576900:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG - chr4_1:81059054-81059073 Msa0576900:CDS 30.0%
! TGATAGTATTGAGTCATGTA+GGG + chr4_1:81059233-81059252 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG + chr4_1:81059234-81059253 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG + chr4_1:81059277-81059296 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG + chr4_1:81059319-81059338 None:intergenic 30.0%
TGTTATCATACTTGTCAACT+TGG - chr4_1:81059791-81059810 Msa0576900:five_prime_UTR 30.0%
AAGGATTGAATGTTTAGAAG+TGG + chr4_1:81057595-81057614 None:intergenic 30.0%
GATATTTAATTGTAGGAAGG+AGG + chr4_1:81057649-81057668 None:intergenic 30.0%
TTGACTGATCTTCAAAACTT+TGG - chr4_1:81057674-81057693 Msa0576900:three_prime_UTR 30.0%
ATGAAACAAGTAAGGAAATG+GGG + chr4_1:81057970-81057989 None:intergenic 30.0%
GCTAAACACGATTTAATAGA+AGG - chr4_1:81058076-81058095 Msa0576900:CDS 30.0%
GGAATTTGTACTAAAATGAC+AGG + chr4_1:81058390-81058409 None:intergenic 30.0%
CAGGAAAATGAAATCATTGT+TGG + chr4_1:81058511-81058530 None:intergenic 30.0%
TTCCTGAGTAAGTATACAAT+TGG - chr4_1:81058525-81058544 Msa0576900:intron 30.0%
!!! CCGTCCATTTTAATTAACAA+AGG + chr4_1:81058584-81058603 None:intergenic 30.0%
ATTCAAATAACATCACTCCA+TGG + chr4_1:81058655-81058674 None:intergenic 30.0%
ATACATGACAATGTGTGAAA+TGG - chr4_1:81058764-81058783 Msa0576900:intron 30.0%
AAACCAAAACATGTCTCAAT+CGG - chr4_1:81058909-81058928 Msa0576900:intron 30.0%
TGAGCAAATTGAGAAAAACT+TGG - chr4_1:81059054-81059073 Msa0576900:CDS 30.0%
! TGATAGTATTGAGTCATGTA+GGG + chr4_1:81059233-81059252 None:intergenic 30.0%
! ATGATAGTATTGAGTCATGT+AGG + chr4_1:81059234-81059253 None:intergenic 30.0%
AACAGATAACACAAGCATTT+GGG + chr4_1:81059277-81059296 None:intergenic 30.0%
ATTCTATGCTTTCTCAATCA+AGG + chr4_1:81059319-81059338 None:intergenic 30.0%
TGTTATCATACTTGTCAACT+TGG - chr4_1:81059791-81059810 Msa0576900:five_prime_UTR 30.0%
TGAATGTTTAGAAGTGGAGA+AGG + chr4_1:81057589-81057608 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG + chr4_1:81057618-81057637 None:intergenic 35.0%
GTAGAAAATAGTGGTGAAGT+TGG - chr4_1:81058241-81058260 Msa0576900:CDS 35.0%
AAAGCAGAAAGCCATGAAAT+CGG - chr4_1:81058283-81058302 Msa0576900:CDS 35.0%
GTCCAATTGTATACTTACTC+AGG + chr4_1:81058530-81058549 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG - chr4_1:81058635-81058654 Msa0576900:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG - chr4_1:81058715-81058734 Msa0576900:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG - chr4_1:81058716-81058735 Msa0576900:intron 35.0%
ACACATTGTCATGTATGAGT+CGG + chr4_1:81058760-81058779 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG - chr4_1:81058769-81058788 Msa0576900:intron 35.0%
AAATGGGACAACTACAAGAT+AGG + chr4_1:81058841-81058860 None:intergenic 35.0%
ATTTGCTCACAACATCAACA+GGG + chr4_1:81059043-81059062 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG + chr4_1:81059044-81059063 None:intergenic 35.0%
ATTGAGAAAAACTTGGCACA+CGG - chr4_1:81059061-81059080 Msa0576900:CDS 35.0%
! TTGGCACACGGAAATTTTAA+CGG - chr4_1:81059073-81059092 Msa0576900:intron 35.0%
!! ACACGGAAATTTTAACGGTT+GGG - chr4_1:81059078-81059097 Msa0576900:intron 35.0%
! AGCTCAGCAATTTGTTCATT+TGG + chr4_1:81059185-81059204 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG - chr4_1:81059200-81059219 Msa0576900:intron 35.0%
CAACAGATAACACAAGCATT+TGG + chr4_1:81059278-81059297 None:intergenic 35.0%
AGTGGTCATTGGATTGAAAT+TGG - chr4_1:81059396-81059415 Msa0576900:intron 35.0%
ATCATTGGATTGAGATCACA+CGG + chr4_1:81059486-81059505 None:intergenic 35.0%
TCTCTGACTACATGAATGTA+TGG - chr4_1:81059514-81059533 Msa0576900:intron 35.0%
TTGGACATGACCAAAAAGTA+AGG - chr4_1:81059595-81059614 Msa0576900:intron 35.0%
!! CGCAAACATTCCTTACTTTT+TGG + chr4_1:81059608-81059627 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG + chr4_1:81059683-81059702 None:intergenic 35.0%
TGAATGTTTAGAAGTGGAGA+AGG + chr4_1:81057589-81057608 None:intergenic 35.0%
GAAAAATTAAGAGAGGGAGT+AGG + chr4_1:81057618-81057637 None:intergenic 35.0%
GTAGAAAATAGTGGTGAAGT+TGG - chr4_1:81058241-81058260 Msa0576900:CDS 35.0%
AAAGCAGAAAGCCATGAAAT+CGG - chr4_1:81058283-81058302 Msa0576900:CDS 35.0%
GTCCAATTGTATACTTACTC+AGG + chr4_1:81058530-81058549 None:intergenic 35.0%
CATGTGCACTTATTTAACCA+TGG - chr4_1:81058635-81058654 Msa0576900:intron 35.0%
! ATTTGAACAACCGTTCGTTT+TGG - chr4_1:81058715-81058734 Msa0576900:intron 35.0%
! TTTGAACAACCGTTCGTTTT+GGG - chr4_1:81058716-81058735 Msa0576900:intron 35.0%
ACACATTGTCATGTATGAGT+CGG + chr4_1:81058760-81058779 None:intergenic 35.0%
TGACAATGTGTGAAATGGTA+TGG - chr4_1:81058769-81058788 Msa0576900:intron 35.0%
AAATGGGACAACTACAAGAT+AGG + chr4_1:81058841-81058860 None:intergenic 35.0%
ATTTGCTCACAACATCAACA+GGG + chr4_1:81059043-81059062 None:intergenic 35.0%
! AATTTGCTCACAACATCAAC+AGG + chr4_1:81059044-81059063 None:intergenic 35.0%
ATTGAGAAAAACTTGGCACA+CGG - chr4_1:81059061-81059080 Msa0576900:CDS 35.0%
! TTGGCACACGGAAATTTTAA+CGG - chr4_1:81059073-81059092 Msa0576900:intron 35.0%
!! ACACGGAAATTTTAACGGTT+GGG - chr4_1:81059078-81059097 Msa0576900:intron 35.0%
! AGCTCAGCAATTTGTTCATT+TGG + chr4_1:81059185-81059204 None:intergenic 35.0%
GAGCTTATTAAGCAATACAG+AGG - chr4_1:81059200-81059219 Msa0576900:intron 35.0%
CAACAGATAACACAAGCATT+TGG + chr4_1:81059278-81059297 None:intergenic 35.0%
AGTGGTCATTGGATTGAAAT+TGG - chr4_1:81059396-81059415 Msa0576900:intron 35.0%
ATCATTGGATTGAGATCACA+CGG + chr4_1:81059486-81059505 None:intergenic 35.0%
TCTCTGACTACATGAATGTA+TGG - chr4_1:81059514-81059533 Msa0576900:intron 35.0%
TTGGACATGACCAAAAAGTA+AGG - chr4_1:81059595-81059614 Msa0576900:intron 35.0%
!! CGCAAACATTCCTTACTTTT+TGG + chr4_1:81059608-81059627 None:intergenic 35.0%
AATACACCATAATCCTCATC+AGG + chr4_1:81059683-81059702 None:intergenic 35.0%
!!! GGCACTTGTTTGACTTGTTT+TGG - chr4_1:81057486-81057505 Msa0576900:three_prime_UTR 40.0%
TTCGTGTTGGGACAATATGT+AGG + chr4_1:81057543-81057562 None:intergenic 40.0%
ATGGAATGCAACTTCGTGTT+GGG + chr4_1:81057555-81057574 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG + chr4_1:81057614-81057633 None:intergenic 40.0%
TTGGACTCGAGTGAAAATGA+AGG - chr4_1:81057693-81057712 Msa0576900:three_prime_UTR 40.0%
! AAGGCTAGAAACCGATTTCA+TGG + chr4_1:81058297-81058316 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG - chr4_1:81058299-81058318 Msa0576900:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG - chr4_1:81058717-81058736 Msa0576900:intron 40.0%
! TGTCCGATTGAGACATGTTT+TGG + chr4_1:81058915-81058934 None:intergenic 40.0%
!! CACACGGAAATTTTAACGGT+TGG - chr4_1:81059077-81059096 Msa0576900:intron 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG + chr4_1:81059116-81059135 None:intergenic 40.0%
CTATCATGTTCAGAGTGTAG+AGG - chr4_1:81059247-81059266 Msa0576900:intron 40.0%
TCAGAGACGTCACTAATCAT+TGG + chr4_1:81059501-81059520 None:intergenic 40.0%
TGAGCTCACCAATTCAACTA+TGG + chr4_1:81059565-81059584 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG - chr4_1:81059566-81059585 Msa0576900:intron 40.0%
GTGAGCTCAATGGAATTGAT+TGG - chr4_1:81059576-81059595 Msa0576900:intron 40.0%
ATGGCACAATGAACAAGTCT+TGG + chr4_1:81059761-81059780 None:intergenic 40.0%
!!! GGCACTTGTTTGACTTGTTT+TGG - chr4_1:81057486-81057505 Msa0576900:three_prime_UTR 40.0%
TTCGTGTTGGGACAATATGT+AGG + chr4_1:81057543-81057562 None:intergenic 40.0%
ATGGAATGCAACTTCGTGTT+GGG + chr4_1:81057555-81057574 None:intergenic 40.0%
AATTAAGAGAGGGAGTAGGA+AGG + chr4_1:81057614-81057633 None:intergenic 40.0%
TTGGACTCGAGTGAAAATGA+AGG - chr4_1:81057693-81057712 Msa0576900:three_prime_UTR 40.0%
! AAGGCTAGAAACCGATTTCA+TGG + chr4_1:81058297-81058316 None:intergenic 40.0%
!! AAATCGGTTTCTAGCCTTTC+AGG - chr4_1:81058299-81058318 Msa0576900:CDS 40.0%
! TTGAACAACCGTTCGTTTTG+GGG - chr4_1:81058717-81058736 Msa0576900:intron 40.0%
! TGTCCGATTGAGACATGTTT+TGG + chr4_1:81058915-81058934 None:intergenic 40.0%
!! CACACGGAAATTTTAACGGT+TGG - chr4_1:81059077-81059096 Msa0576900:intron 40.0%
!!! CCTTCGTCTTTTTTCTTCTC+CGG + chr4_1:81059116-81059135 None:intergenic 40.0%
CTATCATGTTCAGAGTGTAG+AGG - chr4_1:81059247-81059266 Msa0576900:intron 40.0%
TCAGAGACGTCACTAATCAT+TGG + chr4_1:81059501-81059520 None:intergenic 40.0%
TGAGCTCACCAATTCAACTA+TGG + chr4_1:81059565-81059584 None:intergenic 40.0%
AGTTGAATTGGTGAGCTCAA+TGG - chr4_1:81059566-81059585 Msa0576900:intron 40.0%
GTGAGCTCAATGGAATTGAT+TGG - chr4_1:81059576-81059595 Msa0576900:intron 40.0%
ATGGCACAATGAACAAGTCT+TGG + chr4_1:81059761-81059780 None:intergenic 40.0%
GATGGAATGCAACTTCGTGT+TGG + chr4_1:81057556-81057575 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG + chr4_1:81057752-81057771 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG - chr4_1:81058326-81058345 Msa0576900:CDS 45.0%
ACAGTTGTCCCCAAAACGAA+CGG + chr4_1:81058728-81058747 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG - chr4_1:81058977-81058996 Msa0576900:intron 45.0%
TTCTATCGCCATGGACATGA+AGG - chr4_1:81058986-81059005 Msa0576900:intron 45.0%
CTTCTTGTCCTTCATGTCCA+TGG + chr4_1:81058997-81059016 None:intergenic 45.0%
!! GGAAATTTTAACGGTTGGGC+CGG - chr4_1:81059082-81059101 Msa0576900:intron 45.0%
CCGGAGAAGAAAAAAGACGA+AGG - chr4_1:81059113-81059132 Msa0576900:intron 45.0%
!! CATGTTATTAGGAGCGTCAG+TGG - chr4_1:81059378-81059397 Msa0576900:intron 45.0%
TAGATTGAGACGTCCTGATG+AGG - chr4_1:81059667-81059686 Msa0576900:five_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG - chr4_1:81059674-81059693 Msa0576900:five_prime_UTR 45.0%
GATGGAATGCAACTTCGTGT+TGG + chr4_1:81057556-81057575 None:intergenic 45.0%
ATGGTCGTGTACTCTTGCTT+AGG + chr4_1:81057752-81057771 None:intergenic 45.0%
CACTGGTCACTACACTACTA+AGG - chr4_1:81058326-81058345 Msa0576900:CDS 45.0%
ACAGTTGTCCCCAAAACGAA+CGG + chr4_1:81058728-81058747 None:intergenic 45.0%
!! AGGCATTGATTCTATCGCCA+TGG - chr4_1:81058977-81058996 Msa0576900:intron 45.0%
TTCTATCGCCATGGACATGA+AGG - chr4_1:81058986-81059005 Msa0576900:intron 45.0%
CTTCTTGTCCTTCATGTCCA+TGG + chr4_1:81058997-81059016 None:intergenic 45.0%
!! GGAAATTTTAACGGTTGGGC+CGG - chr4_1:81059082-81059101 Msa0576900:intron 45.0%
CCGGAGAAGAAAAAAGACGA+AGG - chr4_1:81059113-81059132 Msa0576900:intron 45.0%
!! CATGTTATTAGGAGCGTCAG+TGG - chr4_1:81059378-81059397 Msa0576900:intron 45.0%
TAGATTGAGACGTCCTGATG+AGG - chr4_1:81059667-81059686 Msa0576900:five_prime_UTR 45.0%
AGACGTCCTGATGAGGATTA+TGG - chr4_1:81059674-81059693 Msa0576900:five_prime_UTR 45.0%
!! ATATCAAAAATAAATAATAA+AGG - chr4_1:81058814-81058833 Msa0576900:intron 5.0%
!! ATATCAAAAATAAATAATAA+AGG - chr4_1:81058814-81058833 Msa0576900:intron 5.0%
GGAGAAGGAGCAGAGAAAGA+TGG + chr4_1:81057574-81057593 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG - chr4_1:81058309-81058328 Msa0576900:CDS 50.0%
GTGACCAGTGAGTACCTGAA+AGG + chr4_1:81058316-81058335 None:intergenic 50.0%
GAAGGACAAGAAGCTGACAG+TGG - chr4_1:81059004-81059023 Msa0576900:intron 50.0%
GACAAGAAGCTGACAGTGGT+GGG - chr4_1:81059008-81059027 Msa0576900:intron 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG + chr4_1:81059104-81059123 None:intergenic 50.0%
!! TTAGGAGCGTCAGTGGTCAT+TGG - chr4_1:81059385-81059404 Msa0576900:intron 50.0%
GGAGAAGGAGCAGAGAAAGA+TGG + chr4_1:81057574-81057593 None:intergenic 50.0%
! CTAGCCTTTCAGGTACTCAC+TGG - chr4_1:81058309-81058328 Msa0576900:CDS 50.0%
GTGACCAGTGAGTACCTGAA+AGG + chr4_1:81058316-81058335 None:intergenic 50.0%
GAAGGACAAGAAGCTGACAG+TGG - chr4_1:81059004-81059023 Msa0576900:intron 50.0%
GACAAGAAGCTGACAGTGGT+GGG - chr4_1:81059008-81059027 Msa0576900:intron 50.0%
! TTCTTCTCCGGCTCTTTTGC+CGG + chr4_1:81059104-81059123 None:intergenic 50.0%
!! TTAGGAGCGTCAGTGGTCAT+TGG - chr4_1:81059385-81059404 Msa0576900:intron 50.0%
GGACAAGAAGCTGACAGTGG+TGG - chr4_1:81059007-81059026 Msa0576900:intron 55.0%
GGACAAGAAGCTGACAGTGG+TGG - chr4_1:81059007-81059026 Msa0576900:intron 55.0%
GGTTGGGCCGGCAAAAGAGC+CGG - chr4_1:81059094-81059113 Msa0576900:intron 65.0%
GGTTGGGCCGGCAAAAGAGC+CGG - chr4_1:81059094-81059113 Msa0576900:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81057482 81059849 81057482 ID=Msa0576900;Name=Msa0576900
chr4_1 mRNA 81057482 81059849 81057482 ID=Msa0576900-mRNA-1;Parent=Msa0576900;Name=Msa0576900-mRNA-1;_AED=0.07;_eAED=0.07;_QI=227|1|1|1|1|1|3|551|133
chr4_1 exon 81057482 81058352 81057482 ID=Msa0576900-mRNA-1:exon:29144;Parent=Msa0576900-mRNA-1
chr4_1 exon 81059011 81059086 81059011 ID=Msa0576900-mRNA-1:exon:29143;Parent=Msa0576900-mRNA-1
chr4_1 exon 81059617 81059849 81059617 ID=Msa0576900-mRNA-1:exon:29142;Parent=Msa0576900-mRNA-1
chr4_1 five_prime_UTR 81059623 81059849 81059623 ID=Msa0576900-mRNA-1:five_prime_utr;Parent=Msa0576900-mRNA-1
chr4_1 CDS 81059617 81059622 81059617 ID=Msa0576900-mRNA-1:cds;Parent=Msa0576900-mRNA-1
chr4_1 CDS 81059011 81059086 81059011 ID=Msa0576900-mRNA-1:cds;Parent=Msa0576900-mRNA-1
chr4_1 CDS 81058033 81058352 81058033 ID=Msa0576900-mRNA-1:cds;Parent=Msa0576900-mRNA-1
chr4_1 three_prime_UTR 81057482 81058032 81057482 ID=Msa0576900-mRNA-1:three_prime_utr;Parent=Msa0576900-mRNA-1
Gene Sequence

>Msa0576900

ATGAAGAAAATAGTGGTGAAGTTGGACTTGCATGATGACAAAGCAAAGCAGAAAGCCATGAAATCGGTTTCTAGCCTTTCAGGCATTGATTCTATCGCCATGGACATGAAGGACAAGAAGCTGACAGTGGTGGGTAACATAGACCCTGTTGATGTTGTGAGCAAATTGAGAAAAACTTGGCACACGGAAATTTTAACGGTTGGGCCGGCAAAAGAGCCGGAGAAGAAAAAAGACGAAGGCAAAAAAGATGATAATAAGAAGAAAGAAGATGACAAGAAGAAAGATCCAAATGAACAAATTGCTGAGCTTATTAAGCAATACAGAGGATACAATCCCTACATGACTCAATACTATCATGTTCAGAGTGTAGAGGAGAACCCAAATGCTTGTGTTATCTGTTGA
Protein sequence

>Msa0576900

MKKIVVKLDLHDDKAKQKAMKSVSSLSGIDSIAMDMKDKKLTVVGNIDPVDVVSKLRKTWHTEILTVGPAKEPEKKKDEGKKDDNKKKEDDKKKDPNEQIAELIKQYRGYNPYMTQYYHVQSVEENPNACVIC*